Pei-Gen Xiao

Chinese Academy of Medical Sciences, Peping, Beijing, China

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Publications (116)74.31 Total impact

  • Chemistry & Biodiversity 09/2014; 11(9):1277-97. · 1.81 Impact Factor
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    ABSTRACT: Genus Kadsura Kaempf. ex Juss. includes important medicinal plants widely used in the south and southwest of China. The stems and roots are effective in activating blood and resolving stasis, promoting qi circulation to relieve pain, dispelling wind and eliminating dampness, in which lignans and triterpenoids are the major bioactive constituents. Here we summarized the chemical constituents isolated from genus Kadsura Kaempf. ex Juss., which would provide a primary and strategic platform for further exploiting the medicinal value and resources of genus Kadsura Kaempf. ex Juss.
    Chinese Herbal Medicines. 08/2014; 6(3):172–197.
  • Chemistry & Biodiversity 03/2014; 11(1):55-72. · 1.81 Impact Factor
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    ABSTRACT: The tribe Chelidonieae comprises 23 species of eight genera with an extensive distribution and a long medicinal usage history both in China and Western countries. A large number of chemical constituents have been isolated and identified from the species in tribe Chelidonieae, such as alkaloids, organic acids, and their derivatives, aromatics, triterpenoids, sterols, essential oils, and proteins, most of which possess a variety of bioactivities, especially for the antibacterial, anti-inflammation, antitumor, analgesia, anti-oxidation, and antiparasitic activity. Meanwhile, potential toxicities have been discovered in some constituents. Therefore, the species in tribe Chelidonieae have become a rich source for new drug discovery, biologic study, and mechanism research. This paper presents comprehensive information of the chemical constituents, pharmacological and toxicological research on the plants in tribe Chelidoieae, which is a reference for the plants in this tribe for further development.
    Chinese Herbal Medicines. 02/2014; 6(1):1–21.
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    ABSTRACT: 6-Hydroxydopamine (6-OHDA) is a neurotoxin that is commonly employed to induce lesions of the dopaminergic pathways to generating experimental models of Parkinson’s disease (PD) in rodents. Antioxidant and anti-inflammatory therapy approaches have been the focus of attention in the treatment of neurodegenerative. PD and Alzheimer’s diseases, and oxidative stress have been implicated in these diseases. In this study, we investigated the neuroprotective effects of minocycline and the signalling pathway that is possibly involved in a PC12 cell model of PD. The results indicated that 6-OHDA cytotoxicity was accompanied by an increment in lactate dehydrogenase (LDH) release, an increase in caspase-3 protein activity, an increase in ROS generation, MDA content and decrease in the SOD, CAT activities and cell viability. Moreover, treatment with 6-OHDA alone for 24 h resulted in ICAD degradation, increased nuclear translocation of NF-κB, and increased p53 expression. However, pretreatment with minocycline (5, 10, 20 µM) for 24 h significantly reduced LDH release, reduced caspase-3 protein production, reduced ROS production, MDA content and attenuated the decrease in SOD, CAT activities and cell viability. Additionally, minocycline (20 µM) markedly decreased the levels of cleaved ICAD protein, down-regulated p53 activity and inhibited the nuclear translocation of NF-κB. The neuroprotective effects of minocycline were attributable to its potent antioxidant activities, which prevented the nuclear translocation of NF-κB and the subsequent promotion of cell death. Therefore, the present study supports the notion that minocycline may be a promising neuroprotective agent for the treatment of Parkinson’s disease.
    Brain Research. 01/2014;
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    ABSTRACT: To study the relationship between ultra-performance liquid chromatography (UPLC) fingerprints of Rheum species and their anti-HIV 1 activities. Twenty two samples of 16 species belonging to genus Rheum from various sources were collected and analyzed in this study. Firstly they were assayed for the HIV-1 reverse transcriptase (RT) associated ribonuclease H (RNase H) activity. Secondly the fingerprints were established by an optimized UPLC method. Sample was analyzed by UPLC-TOF-MS/MS to identify major peaks. The possible relationship between UPLC fingerprints and anti-HIV 1 activities of Rheum species were deduced by mathematical statistics method. Samples of R. austral, R. austral, R. hotaoense exhibited good anti-HIV 1 activities with IC50 < or = 0.2 mg x L(-1). The correlation of anti-HIV 1 activities and fingerprints showed that three compounds were the main bioactive components, and their retention times were 4.74, 7.99, 21.18 min, respectively. Compounds in Rheum species with possible anti-HIV 1 activities were deduced by spectrum-effect relationship study. This study supported for study of medicinal plants in Rheum.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 08/2013; 38(15):2434-7.
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    ABSTRACT: The genus Fritillaria is a botanical source for various pharmaceutically active components, which have been commonly used in traditional Chinese medicine for thousands of years. Increasing interest in Fritillaria medicinal resources has led to additional discoveries of steroidal alkaloids, saponins, terpenoids, glycosides and many other compounds in various Fritillaria species, and to investigations on their chemotaxonomy, molecular phylogeny and pharmacology. In continuation of studies on Fritillaria pharmacophylogeny, the phytochemistry, chemotaxonomy, molecular biology and phylogeny of Fritillaria and their relevance to drug efficacy is reviewed. Literature searching is used to characterize the global scientific effort in the flexible technologies being applied. The interrelationship within Chinese Bei Mu species and between Chinese species, and species distributed outside of China, is clarified by the molecular phylogenetic inferences based on nuclear and chloroplast DNA sequences. The incongruence between chemotaxonomy and molecular phylogeny is revealed and discussed. It is essential to study more species for both the sustainable utilization of Fritillaria medicinal resources and for finding novel compounds with potential clinical utility. Systems biology and omics technologies will play an increasingly important role in future pharmaceutical research involving the bioactive compounds of Fritillaria.
    Chinese journal of natural medicines. 07/2013; 11(4):330-344.
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    ABSTRACT: A rapid, sensitive, and accurate HPLC-DAD method was developed and validated for simultaneous determination of one phenolic glycoside and seven monoterpene glycosides, including 1-O-β-d-(4-hydroxybenzoyl)glucose (1), pyridylpaeoniflorin (2), (8R)-piperitone-4-en-9-O-β-d-glucopyranoside (3), oxypaeoniflorin (4), 6'-O-β-glucopyranosylalbiflorin (5), albiflorin (6), β-gentiobiosylpaeoniflorin (7), and paeoniflorin (8), in 44 batches of peony seeds from nine Paeonia species collected from different areas. Using the optimised method, separations were conducted with a YMC-pack ODS-A column with water/formic acid and methanol as the mobile phase. All eight analytes demonstrated good linearity (r(2)>0.9993). The recoveries, measured at three concentration levels, varied from 98.20% to 103.81%. Six compounds including 1 and 4-8 occur ubiquitously in all the seeds of nine Paeonia species, and compounds 2 and 3 showed undetectable levels or very low content in several samples. The seed samples were classified into several groups, which coincide with the taxonomy of Paeonia at the section level. Peony seed might be a useful resource in developing new herbal or food products.
    Food Chemistry 06/2013; 138(4):2108-14. · 3.33 Impact Factor
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    ABSTRACT: Cimicifugeae is one of the rich sources for various active components and the health promoting and therapeutic values of the components have been corroborated by long-term use in folk medicine and traditional Chinese medicine. Increasing interest in Cimicifugeae pharmaceutical resources has led to the further discoveries of triterpenoid saponins, phenolic compounds, chromones, and many other compounds in various species of Cimicifugeae, and to the investigations on their chemotaxonomy, molecular phylogeny, and bioactivities. Based on our pharmacophylogenetic studies, the progress in phytochemistry, chemotaxonomy, molecular biology, and phylogeny of Cimicifugeae had been summarized since 2007, especially Cimicifuga L. ex Wernisch. and Actaea L., and their relevance to therapeutic efficacy. An exhaustive literature survey is used to characterize the global scientific effort in the phytochemical and biological studies of Cimicifugeae. More triterpenoid saponins have been found in various species, among which the cimigenol type (type A) is predominant. The versatile bioactivities of saponins and extracts, as well as those of phenolics and other ingredients, were summarized and discussed. The morphology-based five-genus classification of Cimicifugeae is not supported by molecular phylogeny. Molecular phylogeny based on nuclear and chloroplast DNA sequences tends to merge Cimicifuga Wernisch., Souliea Franch., and Actaea L. into a single genus. It is indispensable to integrate the emerging technologies into Cimicifugeae research for both the sustainable utilization of Cimicifugeae pharmaceutical resources and finding novel compounds with potential clinical utility and less adverse effects. Systems biology and omics technologies would play an increasingly important role in booming pharmaceutical research involving bioactive compounds of Cimicifugeae.
    Chinese Herbal Medicines. 05/2013; 5(2):81–95.
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    ABSTRACT: Dragon's blood is a rare and precious traditional medicine used by different cultures since ancient times. However, studies on enhancing the rapid accumulation of dragon's blood in Dracaena cambodiana and determining its formation mechanism are unavailable. In this study, the activities of two fungi, namely, BJDC01 and BJDC05, and their effect on promoting the accumulation of five main compositions of dragon's blood in D. cambodiana were investigated for the first time. Results of field tests conducted for ten months indicated that the contents of Loureirin D, 4,4'-dihydroxy-2'-dimethoxychalcone, Loureirin A and Loureirin B in two fungal-inoculated materials were 1.67 to 2.85 times greater than those of natural samples, and thus were significantly higher than those of the control groups. The content of 4,4'-dihydroxy-2'6'- dimethoxydihydrochalcone in each fungal-inoculated sample was close to that of the natural sample, and was more than twice of each of the control group. By combining the results of morphological characterizations, both BJDC01 and BJDC05 can stimulate the accumulation of the compositions of dragon's blood. This stimulation may be considered as a defense response of D. cambodiana tree against the invasion of foreign fungi. Thus, this study provides a potential way of producing dragon's blood via the inoculation of two fungal elicitors.
    Fitoterapia 03/2013; · 2.23 Impact Factor
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    ABSTRACT: The rhizome of Anemarrhena asphodeloides is used as food and traditional Chinese medicine for its hypoglycemic effect. The aim of this study was to investigate the isolation, purification and hypoglycemic activity of Anemaran as the active component. The influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. The optimal conditions for NA and AA were extraction temperature 90ºC and 100ºC, duration 1h and 1.5 h, extraction time 3 and 3, and the solid-liquor ratio 1:20 and 1:15, respectively. Neutral and acid Anemaran (NA and AA) were isolated from the rhizome of Anemarrhena asphodeloides. Five fractions of NA-1, NA-2, NA-3, AA-1 and AA-2 were obtained after crude neutral and acid Anemaran purified through DEAE- 52 cellulose anion-exchange column. The characterizations of Anemaran and its different fractions were both analyzed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron micrographs (SEM). Structural properties of different fractions were examined by FT-IR. Strong characteristic absorption peaks were observed at around 1744 cm(-1)and 1650 cm(-1) caused by the C=O group of uronic acids, and the band between 1440 cm(-1) and 1395 cm(-1) associated with the stretching vibration of C-O of galacturonic acid. Neither the crude neutral, nor the acid anemaran significantly inhibited the growth of HepG2 cells in-vitro, which indicated the low cytotoxicity of the anemaran. Furthermore, both neutral and acid anemaran showed hypoglycemic effect. The hypoglycemic effect of neutral anemaran was much higher than that of acid anemaran.
    Iranian journal of pharmaceutical research (IJPR) 01/2013; 12(4):777-88. · 0.54 Impact Factor
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    ABSTRACT: A rapid, sensitive, and accurate HPLC-DAD method was developed and validated for simultaneous determination of 10 stilbenes, which include suffruticosol A, suffruticosol B, suffruticosol C, resveratrol (E)-form, cis--viniferin, trans--viniferin, cis-suffruticosol D, cis-gnetin H, trans-suffruticosol D and gnetin H, in 44 plant samples from 9 different PAEONIA species. In this optimized method, separations were carried out on an YMC-pack ODS-A column with water and methanol as mobile phase. The flow rate was set at 1.0 mL/min and the detector wavelength was set at 230 nm. The results showed that all the 10 analytes showed good linearity (r2 > 0.9994). The recoveries, measured at three concentration levels, varied from 97.19% to 102.89%. This method was also validated with respect to precision, repeatability, stability, and successfully applied to simultaneous determination of 10 stilbenes in 44 batches of peony seeds from 9 PAEONIA species collected from different areas.
    Journal of Liquid Chromatography &amp Related Technologies 01/2013; 36(12). · 0.57 Impact Factor
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    ABSTRACT: In China, many species of genus Salvia L. (Lamiaceae) are important medicinal plants with a long history. Due to their marked ethnopharmacological uses in folk medicine and a large number of active constituents with therapeutic potential found recently, they have become a rich source for new drug discovery, mechanism research, and biological experiment. All medicinal plants of Salvia L. could be divided into three groups (Groups 1, 2, and 3). The plants in Group 1 were used as “Danshen” and are rich in diterpenoids and caffeic acid derivatives. Few introduced plants in Group 2 from America or Europe have been used to clear body-heat and toxins. Abietane diterpenes of medicinal species in Group 3 (the species are from Subg. Allagospadonopsis Briq., Sect. Plethiosphace, or Sect. Notiosphace) are absent, and the contents of caffeic acid derivatives are considerably lower than those in plants of Group 1.
    Chinese Herbal Medicines. 01/2013; 5(3):164–181.
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    ABSTRACT: Objective To document the knowledge and the use of indigenous medicinal plants by traditional healers in Baotou, Inner Mongolia, China.Methods Data were collected from 112 randomly-selected interviewees using semistructured interviews in wild herbal plant collected from 2007 to 2010. The data from the interviewees were analyzed with two quantitative tools. With the informant consensus factor, the information homology was evaluated and with the fidelity level the most important species from the categories were found.ResultsOne hundred and fifty-two species belonging to 112 genera in 48 families with medicinal values were recorded. The reported medicinal plants species were used to treat 63 kinds of diseases. And the medicinal plants in this district possessed significant potentials for their pharmacological activities in the context of ethnopharmacological knowledge, especially in the treatments of gastrointestinal, dermatological, and cardiovascular diseases.Conclusion In this work, 152 medicinal plants with their ethnopharmacological information are reported. This study demonstrates that many species play an important role in healing practices among inhabitants from Baotou. More ethnopharmacological information of Mongolian medicinal plants should be gathered and documented in further studies, which is a fundamental step toward developing efficacious natural drugs for various diseases.
    Chinese Herbal Medicines. 11/2012; 4(4):301–313.
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    ABSTRACT: The dried cane of Kadsura interior (Kadsurae Caulis) is recorded in Chinese Pharmacopoeia 2010 as Dian Jixueteng for the treatment of rheumatism, irregular menstruation, and deficiency of Qi and blood. In this paper, morphological characteristics, chemical constituents, and pharmacological activities in the cane of K. interior were summarized. Moreover, some suggestions about application and quality control of Kadsurae Caulis were proposed in order to provide a theoretical basis for further scientific use.
    Chinese Herbal Medicines. 08/2012; 4(3):177–182.
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    ABSTRACT: Taxus is the source plant of anti-cancer drug paclitaxel and its biosynthetic precursor, analogs and derivatives, which has been studying for decades. There are many endemic Taxus species in China, which have been studied in the field of multiple disciplines. Based on the recent studies of the researchers, this review comments on the study of Taxus biology and chemistry. The bibliometric method is used to quantify the global scientific production of Taxus-related research, and identify patterns and tendencies of Taxus-related articles. Gaps are present in knowledge about the genomics, epigenomics, transcriptomics, proteomics, metabolomics and bioinformatics of Taxus and their endophytic fungi. Systems biology and various omics technologies will play an increasingly important role in the coming decades.
    Yao xue xue bao = Acta pharmaceutica Sinica 07/2012; 47(7):827-35.
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    Da-Cheng Hao, Ling Yang, Pei-Gen Xiao, Ming Liu
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    ABSTRACT: Plant microRNAs (miRNAs) have an impact in the regulation of several biological processes such as development, growth and metabolism by negatively controlling gene expression at the post-transcriptional level. However, the role of these small molecules in the medicinal gymnosperm species Taxus remained elusive. To elucidate the role of miRNAs in Taxus we used a deep sequencing approach to analyze the small RNA and degradome sequence tags of Taxus mairei leaves. For miRNAs, the sequencing library generated 14.9 million short sequences, resulting in 13.1 million clean reads. The library contains predominantly small RNAs with 21 nucleotide length, followed by 19-nt and 20-nt small RNAs. Around 29% of total small RNAs are matched to the T. mairei transcriptome. By sequence alignment, we identified 871 mature miRNAs, 15 miRNA* and 869 miRNA precursors representing known plant miRNA families. There are 547 unique small RNA matching the miRNA precursors. We predict 37 candidate novel miRNAs from the unannotated small RNAs that could be mapped to the reference transcriptome. The expression of the selected candidates was for the first time quantified by real-time reverse transcription polymerase chain reaction. The novel miRNA m0034 turns out to be from the intron sequence of the paclitaxel biosynthetic gene taxadiene synthase. The 21 potential targets of nine novel miRNAs are also predicted. Additionally, 56 targets for known miRNA families and 15 targets for novel candidate miRNA families were identified by high-throughput degradome-sequencing approach. It is found that two paclitaxel biosynthetic genes, taxane 13α hydroxylase and taxane 2α-O-benzoyltransferase, are the cleavage targets of miR164 and miR171, respectively. This study represents the first transcriptome-based analysis of miRNAs and degradome in gymnosperms.
    Physiologia Plantarum 06/2012; 9999(9999). · 3.66 Impact Factor
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    ABSTRACT: Abundant nsSNPs have been found in genes coding for human solute carrier (SLC) transporters, but there is little known about the relationship between the genotype and phenotype of nsSNPs in these membrane proteins. It is unknown which prediction method is better suited for the prediction of nonneutral nsSNPs of SLC transporters. We have identified 2,958 validated nsSNPs in human SLC family members 1-47 from the Ensembl genome database and the NCBI SNP database. Using three different algorithms, 37-45 % of nsSNPs in SLC genes were predicted to have functional impacts on transporter function. Predictions largely agreed with the available experimental annotations. Overall, 76.5, 74.4, and 73.5 % of nonneutral nsSNPs were predicted correctly as damaging by SNAP, SIFT, and PolyPhen, respectively, while 67.4, 66.3, and 76.7 % of neutral nsSNPs were predicted correctly as nondamaging by the three methods, respectively. This study identified many amino acids that were likely to be functionally critical but have not yet been studied experimentally. There was a significant concordance between the predicted results of different methods. Evolutionarily nonneutral (destabilizing) amino acid substitutions are predicted to be the basis for the pathogenic alteration of SLC transporter activity that is associated with disease susceptibility and altered drug/xenobiotic response.
    European Journal of Drug Metabolism and Pharmacokinetics 05/2012; · 1.31 Impact Factor
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    ABSTRACT: Three new compounds including one flavonol glycoside, irisdichotin A (1), and two flavanonol glycosides, irisdichotins B (2) and C (3), were isolated from the rhizomes of Iris dichotoma Pall. Their structures were elucidated on the basis of spectroscopic methods.
    Journal of Asian natural products research 08/2011; 13(8):744-8. · 0.61 Impact Factor
  • Chemistry & Biodiversity 06/2011; 8(6):976-1010. · 1.81 Impact Factor

Publication Stats

317 Citations
74.31 Total Impact Points

Institutions

  • 2003–2014
    • Chinese Academy of Medical Sciences
      Peping, Beijing, China
    • Peking Union Medical College Hospital
      Peping, Beijing, China
  • 2012–2013
    • Dalian Jiaotong University
      Lü-ta-shih, Liaoning, China
  • 2010
    • Institute of Medicinal Plant Development
      Peping, Beijing, China
  • 2007–2009
    • China Academy of Chinese Medical Sciences
      • Institute of Chinese Material Medica
      Beijing, Beijing Shi, China
    • Nanjing University of Traditional Chinese Medicine
      • Department of Chinese Pharmacy
      Peping, Beijing, China
    • Tianjin Institute of Pharmaceutical Research
      T’ien-ching-shih, Tianjin Shi, China
  • 2005–2007
    • Beijing University of Chinese Medicine and Pharmacology
      • • School of Chinese Pharmacy
      • • School of Chinese Pharmacology
      Peping, Beijing, China
    • Livzon Pharmaceutical Group Co., Ltd.
      Peping, Beijing, China
  • 2003–2007
    • Tianjin University
      • School of Pharmaceutical Science and Technology
      Tianjin, Tianjin Shi, China
  • 2006
    • The Hong Kong Polytechnic University
      • Department of Applied Biology and Chemical Technology
      Hong Kong, Hong Kong
  • 2003–2006
    • The University of Hong Kong
      • Department of Biochemistry
      Hong Kong, Hong Kong
  • 2003–2004
    • Shanghai Jiao Tong University
      • School of Pharmacy
      Shanghai, Shanghai Shi, China