Ichiro Kurokawa

Meiwa Hospital, Nishinomiya, Hyōgo, Japan

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Publications (126)309.2 Total impact

  • I Kurokawa, M Ohyama
    British Journal of Dermatology 04/2015; 172(4):851. DOI:10.1111/bjd.13654 · 4.10 Impact Factor
  • International journal of dermatology 06/2014; 53(9). DOI:10.1111/ijd.12413 · 1.23 Impact Factor
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    ABSTRACT: Seborrheic keratosis (SK) is classified into six types: hyperkeratotic; acanthotic; irritated; clonal; reticulated; and adenoid. However, the origins of the respective types of SK remain unclear. To clarify the histogenetic origins of SK, we performed immunohistochemical studies of keratin (K) and filaggrin expression, taking into account the histopathological classifications of SK. Hyperkeratotic SK mainly expressed K1, K10, and filaggrin. Acanthotic SK mainly expressed K14 with some K15. Irritated SK mainly expressed K14 and K17 in squamous eddies. Clonal SK, reticulated SK, and adenoid SK mainly expressed K14. The results show that hyperkeratotic SK differentiated towards squamoid terminal keratinization, whereas acanthotic, irritated, clonal, reticulated, and adenoid SK mainly differentiated towards basaloid undifferentiated cells. In addition, acanthotic SK differentiated towards the hair bulge, and irritated SK differentiated towards the follicular infrainfundibulum. Based on the patterns of keratin and filaggrin expression demonstrated by the histopathological types, SK demonstrated diverse differentiation towards epidermal keratinization, basaloid cells, the infrainfundibulum and hair follicle bulges, which suggests that SK is in an undifferentiated and hyperproliferative state with heterogeneous differentiation. The immunohistochemical method of investigating patterns of keratin expression is useful in the differential diagnosis of cutaneous epithelial tumors.
    International journal of dermatology 06/2013; 53(6). DOI:10.1111/j.1365-4632.2012.05828.x · 1.23 Impact Factor
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    ABSTRACT: Acne vulgaris is the most common disorder of the pilosebaceous unit leading to inflamed skin characterized by the formation of comedones, papules, pustules and scarring. There is increasing evidence that the abundance of Propionibacterium acnes (P. acnes) in the inflamed acne lesions triggers inflammation. Therefore, in addition to treatment with retinoids, the use of antimicrobial agents has been established as a treatment option for acne. This indicates that antimicrobial mechanisms to control the growth of P. acnes may have an important influence on the severity of inflammatory acne. One import antimicrobial innate defense system comprises the production of antimicrobial peptides (AMP), small molecules with a broad spectrum of antimicrobial activity as well as immunomodulatory properties. Although the role of AMP in acne is still emerging, there is increasing evidence that AMP may be of importance in acne. The aim of this viewpoint is to provide some hypotheses about the potential function of AMP in the pathogenesis of acne and to discuss potential AMP-based therapies for the treatment of acne.
    Experimental Dermatology 06/2013; 22(6):386-391. DOI:10.1111/exd.12159 · 4.12 Impact Factor
  • Naoki Oiso, Ichiro Kurokawa, Akira Kawada
    Journal of Dermatological Science 02/2013; 69(2):e50. DOI:10.1016/j.jdermsci.2012.11.454 · 3.34 Impact Factor
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    ABSTRACT: Abstract is missing (Letter).
    Acta Dermato-Venereologica 12/2012; 93(4). DOI:10.2340/00015555-1515 · 4.24 Impact Factor
  • Ichiro Kurokawa, Makoto Kondo, Shigehiro Akachi
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    ABSTRACT: We studied the suitability of a PCR method using samples of skin and whole blood and serological tests for the early diagnosis of Japan spotted fever (JSF) in its acute and convalescent stages and compared the advantages and disadvantages of these different diagnostic methods. In the acute stage, the percentage of positive results was 91.2 % for the PCR method using skin samples, 52.3 % for the PCR method using whole blood samples, and 40.4 % for the serological tests with IgM. In the convalescent stage, paired serum showed positive results (IgM, 98.5 %; IgC, 94.0 %). The PCR method using samples of skin (eschar) is the most sensitive, specific, and suitable method for promptly and accurately diagnosing JSF in the early stage. Therefore, this method is recommended for early definite diagnosis of JSF in the critical stage.
    Journal of Infection and Chemotherapy 12/2012; DOI:10.1007/s10156-012-0529-x · 1.38 Impact Factor
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    ABSTRACT: We performed immunohistochemical studies of epithelial keratins in intraductal carcinoma in situ (IDCIS) in mammary Paget's disease (MPD). K7, K8 and K18 were expressed in IDCIS in MPD. However, K19 was not expressed in IDCIS in MPD. Interestingly, K17 was expressed in some tumor cells in IDCIS. K17, a hyperproliferative keratin, may suggest ductal invasion and poor prognosis in MPD.
    Case Reports in Oncology 05/2012; 5(2):400-3. DOI:10.1159/000341663
  • Clinical and Experimental Dermatology 03/2012; 37(2):194-5. DOI:10.1111/j.1365-2230.2011.04204.x · 1.23 Impact Factor
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    Naoki Oiso, Ichiro Kurokawa, Akira Kawada
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    ABSTRACT: The nail unit is constructed by distinctly regulated components. The nail isthmus is a lately proposed region as a transitional zone between the most distal part of the nail bed and the hyponychium. It is difficult to recognize the nail isthmus in the normal nail, but it is easy to identify the region in nail disorders such as pterygium inversum unguis and ectopic nail. We describe structure and putative function of the nail isthmus via histopathologic features of pterygium inversum unguis and ectopic nail.
    Dermatology Research and Practice 01/2012; 2012:925023. DOI:10.1155/2012/925023
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    ABSTRACT: Malignant melanoma (MM) is an aggressive cutaneous malignancy associated with poor prognosis; many putatively therapeutic agents have been administered, but with mostly unsuccessful results. Propionibacterium acnes (P. acnes) is an aerotolerant anaerobic gram-positive bacteria that causes acne and inflammation. After being engulfed and processed by phagocytes, P. acnes induces a strong Th1-type cytokine immune response by producing cytokines such as IL-12, IFN-γ and TNF-α. The characteristic Th2-mediated allergic response can be counteracted by Th1 cytokines induced by P. acnes injection. This inflammatory response induced by P. acnes has been suggested to have antitumor activity, but its effect on MM has not been fully evaluated. We analyzed the anti-tumor activity of P. acnes vaccination in a mouse model of MM. Intratumoral administration of P. acnes successfully protected the host against melanoma progression in vivo by inducing both cutaneous and systemic Th1 type cytokine expression, including TNF-α and IFN-γ, which are associated with subcutaneous granuloma formation. P. acnes-treated tumor lesions were infiltrated with TNF-α and IFN-γ positive T cells. In the spleen, TNF-α as well as IFN-γ producing CD8+T cells were increased, and interestingly, the number of monocytes was also increased following P. acnes administration. These observations suggest that P. acnes vaccination induces both systemic and local antitumor responses. In conclusion, this study shows that P. acnes vaccination may be a potent therapeutic alternative in MM.
    PLoS ONE 12/2011; 6(12):e29020. DOI:10.1371/journal.pone.0029020 · 3.53 Impact Factor
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    ABSTRACT: Atopic dermatitis (AD) is a recurrent inflammatory skin disease characterized by dominant T-helper (Th) 2 cytokine response. Bacillus Calmette-Guérin (BCG) has been used for preventing tuberculosis, and is regarded as a strong Th1 cytokine inducer. Antigen (Ag) 85B is a secretory protein present in Mycobacterium species that induces Th1 cytokine production. We investigated the effects of combined vaccination of heat-killed BCG (hkBCG) and Mycobacterium kansasii Ag85B in an AD mouse model. For the AD model, keratin 14 promoter-derived caspase-1 overexpressing mice (KCASP1Tg) were used. The mice received a combination therapy of hkBCG at age 3 weeks and Ag85B twice weekly for 11 weeks from the 4th week; Ag85B monotherapy from the 4th week; hkBCG monotherapy at the 3rd week; or control saline. Areas of skin lesions, cytokine mRNA expression and serum interleukin (IL)-18 and immunoglobulin (Ig) E levels were analysed. Inducible Foxp3+  regulatory T cells (iTreg), IL-10-producing T cells (Tr1), and interferon (IFN)-γ/IL-4/IL-17-producing T cells were evaluated in the spleen. Saline-treated mice and hkBCG monotherapy mice spontaneously developed severe dermatitis. However, combined therapy with hkBCG and Ag85B significantly suppressed the development of skin lesions and mast cell infiltrations. Elevations of the serum IgE and IL-18 levels were significantly suppressed with combined therapy. Mice treated with hkBCG and Ag85B had a normal number of iTreg in the spleen, and decreased number of both IL-4- and IL-17-producing CD4+ T cells. The effect of Ag85B monotherapy was limited. Combined vaccination with hkBCG and Ag85B decreases AD skin lesions by inducing regulatory T cells, suggesting that this vaccination is a potent and novel therapeutic strategy for AD.
    British Journal of Dermatology 12/2011; 166(5):953-63. DOI:10.1111/j.1365-2133.2011.10763.x · 4.10 Impact Factor
  • The Journal of Dermatology 11/2011; 38(11):1089-92. DOI:10.1111/j.1346-8138.2010.01169.x · 2.35 Impact Factor
  • The Journal of Dermatology 10/2011; 39(8):744-5. DOI:10.1111/j.1346-8138.2011.01389.x · 2.35 Impact Factor
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    ABSTRACT: The nail unit has a unique structure. It has been recently proposed that the nail isthmus as a transitional zone between the most distal part of the nail bed and the hyponychium. A 7-year-old Japanese boy presented with an ectopic nail, an additional and independent miniature nail on the digital pulp of the right fifth finger. We studied the expression of a series of keratin in longitudinal specimens and showed the histopathological manifestation in the nail isthmus. This region in the ectopic nail is subdivided into 2 parts: a proximal and narrow part anchored to the nail plate and a distal and wide part with a semihard keratinized structure.
    The American Journal of dermatopathology 08/2011; 33(8):841-4. DOI:10.1097/DAD.0b013e3181f96bce · 1.43 Impact Factor
  • Clinical and Experimental Dermatology 08/2011; 37(2):193-4. DOI:10.1111/j.1365-2230.2011.04151.x · 1.23 Impact Factor
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    ABSTRACT: Keratins are a highly diverse family of cytoskeletal proteins and important markers of epithelial cell differentiation. In this review, applying the new keratin nomenclature recently introduced, we summarize and discuss the distribution and significance of keratin patterns in cutaneous epithelial tumors in relation to the epithelial structures of normal human skin. The available literature data show that the analysis of keratin profiles broadens our understanding of the differentiation, nature and histogenetic origin of the various, highly singular epithelial tumors arising in the skin. Moreover, keratins may aid in histological diagnosis and, in certain instances, may be helpful for the recognition of tumor malignancy and aggressiveness. Furthermore, we briefly address the topic of keratin-related skin disorders.
    Experimental Dermatology 03/2011; 20(3):217-28. DOI:10.1111/j.1600-0625.2009.01006.x · 4.12 Impact Factor
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    ABSTRACT: Atopic dermatitis (AD) is a chronic disease characterized by a polarized Th2 immune response. Propionibacterium acnes (P. acnes) has been shown to elicit strong Th1 immune responses. We hypothesized that the host immune response to P. acnes will prevent the development of AD. To demonstrate this hypothesis, we investigated the effect of P. acnes vaccination on AD that occurs in keratin 14/driven caspase-1 transgenic mouse. Vaccination with low dose of P. acnes successfully prevented clinical manifestations in the skin of AD mice associated with systemic and cutaneous increased expression of Th1-type cytokines but without suppression of Th2 cytokines. Interestingly, the numbers of IFN-γ(+) T cells, FoxP3(+) CD4(+) CD25(+) T cells (nTreg) and IL-10(+) T cells (Tr1) were significantly increased in the spleen. P. acnes vaccination has effects to alter the cytokine milieu and may be useful for the improvement of atopic symptom.
    Experimental Dermatology 02/2011; 20(2):157-8. DOI:10.1111/j.1600-0625.2010.01180.x · 4.12 Impact Factor
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    ABSTRACT: An 81-year-old female experiencing high fever, fatigue, and loss of appetite was admitted to our hospital and diagnosed with acute cholecystitis. Her condition did not improve and an eschar and erythema subsequently appeared. We then diagnosed Japanese spotted fever (JSF). She recovered immediately after the administration of minocycline. This case differed from other cases because the patient had a remarkably acute hepatic failure. Considering that the present case might be associated with other factors, we performed a repeat polymerase chain reaction (PCR) test on the patient's blood that had been collected on admission and stored. Epstein-Barr virus (EBV) was detected in her blood by PCR. We consider this case might be associated with EBV.
    International journal of dermatology 12/2010; 49(12):1403-5. DOI:10.1111/j.1365-4632.2010.04584.x · 1.23 Impact Factor
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    ABSTRACT: Allergen-specific immunotherapy (SIT) is currently used for several allergic disorders and IL-10-producing regulatory T cells (Tr1) induced by SIT suppress allergic reactions. We investigated the relation between IL-10 production and acquiring allergy. A prospective study was undertaken to evaluate the effect of SIT on IL-10 production in T cells and other cell fractions in children with pollinosis. In addition, blood samples were collected from non-allergic healthy controls and patients with pollinosis to compare the levels of IL-10 production. PBMC were cultured with pollen peptides or control allergens, and the IL-10 production from monocyte and CD4 T cell was analyzed. Monocytes and CD4 T cells from SIT group of patients produced high levels of IL-10, suggesting that the induction of IL-10 is essential for inducing T cell tolerance. IL-10 production from monocytes and T cells was significantly increased in non-allergic controls compared to patients with pollinosis. This high IL-10 production was observed even when PBMC were stimulated with antigens other than pollen peptides. IL-10 is critical for induction of specific T cell tolerance, and increased production of IL-10 by monocytes and T cells during inflammatory responses or after SIT may influence effector cells in allergy. Present data implicates that the low productivity of IL-10 by monocytes and T cells is closely related with sensitivity to multiple allergens, and resistance to allergic diseases. Augmentation of constitutive IL-10 production from immune system is a potential therapeutic approach for allergic disorders.
    Allergology International 11/2010; 60(1):45-51. DOI:10.2332/allergolint.10-OA-0198

Publication Stats

1k Citations
309.20 Total Impact Points


  • 2012–2014
    • Meiwa Hospital
      Nishinomiya, Hyōgo, Japan
  • 2013
    • Christian-Albrechts-Universität zu Kiel
      Kiel, Schleswig-Holstein, Germany
  • 2006–2012
    • Mie University
      • Department of Dermatology
      Tu, Mie, Japan
  • 2009
    • National Institute of Allergy and Infectious Disease
      Muncie, Indiana, United States
  • 2000–2009
    • Hyogo Prefectural Amagasaki Hospital
      Amagasaki, Hyōgo, Japan
  • 1991–2007
    • Kansai Medical University
      • Department of Dermatology
      Moriguchi, Ōsaka, Japan