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ABSTRACT: This study evaluated profiles of immunoglobulin (Ig; IgA, IgG, IgG1, and IgG2a) response in experimental brucellosis induced with Brucella canis in BALB/c mice during an 8-week infection period. Six- to 8-week-old BALB/c mice (n = 36) were experimentally infected with 1 × 10(9) CFU of B. canis via the intraperitoneal route. Serial serum samples were collected from the mice at 0, 3, 7, 14, 21, 28, 35, 42, 49, and 56 days after inoculation. The sera were tested by the rapid slide agglutination test (RSAT) and 2-mercaptoethanol-RSAT and indirect enzyme-linked immunosorbent assay. Sera tested positive for B. canis by the RSAT and 2-mercaptoethanol-RSAT beginning from 7 days after inoculation until the end of the experiment. The IgA response was detected at 14 days after infection and reached peak levels at 21 days after infection. The IgG antibody responses were detected at 7 days after infection and reached the peak value at 35 days after infection. Data of our study demonstrated IgG2a-dominant responses over IgG1 during the course of infection (p > 0.05).
Vector borne and zoonotic diseases (Larchmont, N.Y.) 11/2010; 10(9):927-30. · 2.61 Impact Factor
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Journal of the American Veterinary Medical Association 02/2007; 230(1):27. · 1.79 Impact Factor
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ABSTRACT: Vertical transmission of Brucella abortus in Sprague-Dawley (SD) rats was verified with microbiologic, serologic, and polymerase chain reaction (PCR) methods. The 38 initially Brucella-free SD rats, weighing 200 to 250 g, were injected subcutaneously with 50 microL of a suspension containing 1 x 10(9) colony-forming units (cfu) of B. abortus biotype 1 Korean isolate. The rats were allowed to mate with uninfected SD rats. The isolate was detected by culture and by AMOS (abortus, melitensis, ovis, suis) PCR in testis tissue of infected male rats and splenic tissue of infected female rats. By 7 d after inoculation, the results of both the rose bengal test (RBT) and the plate agglutination test (PAT) were positive for antibody against B. abortus; the reciprocal antibody titre ranged from 200 to 400 in the 1-mo-old offspring and 800 in their dams. The infected rats directly transmitted Brucella to their breeding partners and offspring. Fetuses of infected dams were found to be infected at 20 d of gestation. These data are discussed in relation to a model for epizootic and zoonotic cases possibly involving wild animals. Additional rigorous experiments are warranted to explore the value of this model in developing measures to prevent congenital brucellosis.
Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire 11/2005; 69(4):305-8. · 0.94 Impact Factor
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ABSTRACT: To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.
Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire 11/2003; 67(4):278-82. · 0.94 Impact Factor
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Journal of the American Veterinary Medical Association 03/2003; 222(3):284. · 1.79 Impact Factor
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ABSTRACT: The purpose of this study was to evaluate the effects of pH on the growth of canine Malassezia pachydermatis isolates in vitro. Yeast growth was monitored by measuring the optical density with a spectrophotometer. The growth of American Type Culture Collection and field strains of M. pachydermatis was optimal between the pH values of 4.0 and 8.0, and inhibited at the ranges of 1.0 to 3.0 and 9.0 to 10.0. An analysis of covariance showed no significant differences among the growth curves at pH levels 5.0 to 8.0. Although specific contrast tests showed that the growth slope at pH 4.0 was significantly different from that at pH 5.0 to 8.0, only small, random differences were found when the growth slope at pH 4.0 was compared to the individual slopes at pH 5.0, 6.0, 7.0, and 8.0. The findings of this study suggest that topical acidifying products could be beneficial therapeutic options for cutaneous yeast infections in dogs.
Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire 02/2003; 67(1):56-9. · 0.94 Impact Factor
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ABSTRACT: To examine the fate of Strongyloides venezuelensis. Mongolian gerbils (Meriones unguicalatus) were orally infected with 1,000 L3 larvae per animal. Altogether, 50 gerbils divided into 5 groups of 10 each were monitored for a period of 570 days to document the kinetics of faecal egg output, adults worm population, morphological development, fecundity, and hematological changes including peripheral blood eosinophilia. This study chronicled a life long parasitism of S. venezuelensis in the gerbil host, and showed that S. venezuelensis infection was quite stable throughout the course of infection and the worms maintained their normal development as evidenced by their body dimension. A progressive loss of body condition of the infected gerbils was observed as the level of infection advanced. However, no detectable pathological changes were observed in the gastrointestinal tract. The present findings indicate that an immunocompetent host, such as the Mongolian gerbil, can serve as a life long carrier model of S. venezuelensis if the worms are not expelled within 570 days after infection.
The Korean Journal of Parasitology 01/2003; 40(4):181-6. · 1.04 Impact Factor
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ABSTRACT: This study determined the extent and duration of cutaneous acidification caused by a single application of four acidifying sprays, vinegar, and water. Multivariate repeated measures analysis of variance revealed a significant difference between the six sprays (F = 15.3; P < or = 0.001). Linear contrast tests showed that the effects of the acidifying sprays were significantly different from vinegar and water (F = 6.0; P < or = 0.001), and vinegar was significantly different from water (F = 13.8; P < or = 0.001). The acidifying sprays decreased cutaneous pH to < 6.0 for a mean range of 50 to 65 hours, while vinegar did so for a mean of 12 hours.
Journal of the American Animal Hospital Association 39(1):29-33. · 0.96 Impact Factor
