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Tissue Antigens 04/2013; · 2.59 Impact Factor
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Tissue Antigens 04/2013; · 2.59 Impact Factor
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Tissue Antigens 03/2013; · 2.59 Impact Factor
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Transfusion Medicine 01/2013; · 1.14 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-C*07:208 allele was different from that of HLA-C*07:02:01:01 by a single-nucleotide substitution at position 475 G>C.
Tissue Antigens 06/2012; 80(3):276-8. · 2.59 Impact Factor
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Tissue Antigens 06/2012; 80(2):198-9. · 2.59 Impact Factor
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Tissue Antigens 03/2012; 80(1):68-70. · 2.59 Impact Factor
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ABSTRACT: Compared with HLA-A*31:01:02, HLA-A*31:56 shows one nucleotide difference at position 706 G>A and HLA-A*31:59 has a single nucleotide polymorphism at position 626 C>T.
Tissue Antigens 03/2012; 79(5):388-9. · 2.59 Impact Factor
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Tissue Antigens 12/2011; 79(4):311-2. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-DQB1*03:03:04 allele was different from that of HLA-DQB1*03:03:02 by a single nucleotide substitution at position 603 C>T.
Tissue Antigens 12/2011; 79(3):214-5. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-C*07:02:25 allele was different from that of HLA-C*07:02:01:01 by a single nucleotide substitution at position 78C>G.
Tissue Antigens 07/2011; 78(6):457-9. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-DRB1*12:27 allele was different from that of HLA-DRB1*12:02:01 by three-nucleotide substitution at position 165A>C, 171G>C, and 175C>T.
Tissue Antigens 07/2011; 78(6):465-6. · 2.59 Impact Factor
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ABSTRACT: The novel HLA-DQB1*06:43 allele differs from HLA-DQB1*06:01:01 in one nucleotide substitution at codon 73 in exon 2.
Tissue Antigens 06/2011; 78(6):461-2. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-B*13:41 allele was different from that of B*13:02:01 at position 538 C>T and 539 T>G, resulting in an amino acid from Leu to Trp at codon 156.
Tissue Antigens 06/2011; 78(5):399-400. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-B*15:25:03 allele was different from that of HLA-B*15:25:01 at position 819 G to A.
Tissue Antigens 04/2011; 78(3):219-20. · 2.59 Impact Factor
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Tissue Antigens 04/2011; 78(3):226-7. · 2.59 Impact Factor
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ABSTRACT: The Diego blood group system plays an important role in transfusion medicine. Genotyping of DI1 and DI2 alleles is helpful for the investigation into haemolytic disease of the newborn (HDN) and for the development of rare blood group databases. Here, we set up a polymerase chain reaction sequence-based typing (PCR-SBT) method for genotyping of Diego blood group alleles.
Specific primers for exon 19 of the solute carrier family 4, anion exchanger, member1 (SLC4A1) gene were designed, and our PCR-SBT method was established and optimized for Diego genotyping. A total of 1053 samples from the Chinese Han population and the family members of a rare proband with DI1/DI1 genotype were investigated by the PCR-SBT method. An allele-specific primer PCR (PCR-ASP) was used to verify the reliability of the PCR-SBT method.
The frequencies of DI1 and DI2 alleles in the Chinese Han population were 0.0247 and 0.9753, respectively. Six new single nucleotide polymorphisms (SNPs) were found in the sequenced regions of the SLC4A1 gene, and four novel SNPs located in the exon 19, in which one SNP could cause an amino acid alteration of Ala858Ser on erythrocyte anion exchanger protein 1. The genotypes for Diego blood group were identical among 41 selected samples with PCR-ASP and PCR-SBT.
The PCR-SBT method can be used in Diego genotyping as a substitute of serological technique when the antisera is lacking and was suitable for screening large numbers of donors in rare blood group databases.
Vox Sanguinis 04/2011; 100(3):317-21. · 2.86 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-B*40:128 has a single nucleotide difference at position 539 T>G compared with that of HLA-B*40:01:01, with an amino acid change from Leu to Arg at codon 156.
Tissue Antigens 03/2011; 77(3):260-1. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-A*11:78N allele was different from that of HLA-A*11:01:01 by two nucleotides deletion at positions 286 and 287, resulting in reading frameshift and has premature stop codon at position 73 in exon 2.
Tissue Antigens 03/2011; 77(3):257-8. · 2.59 Impact Factor
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ABSTRACT: Nucleotide sequence of HLA-A*02:230 allele was different from that of HLA-A*02:03:01 by a single nucleotide substitution at codon 139 (GCA > ACA), resulting in one amino acid change (Ala to Thr).
Tissue Antigens 10/2010; 77(2):150-1. · 2.59 Impact Factor