M Grando

Publications (3)9.89 Total impact

• Source

  Available from: Francesco Emanuelli

  Article: The 1-deoxy-D: -xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine.

  Juri Battilana, Laura Costantini, Francesco Emanuelli, Federica Sevini, Cinzia Segala, Sergio Moser, Riccardo Velasco, Giuseppe Versini, M Stella Grando
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  ABSTRACT: Muscat flavor is a relevant trait both in winemaking and in fresh grape consumption. From a chemical point of view, it is strongly related to the accumulation of monoterpenes in berries. However, knowledge of the genetic mechanisms underlying its regulation is still limited. The objective of this study was to dissect the genetic determinism of aroma in grapevine by applying the analysis of quantitative trait loci (QTL) and the candidate gene (CG) approach. Two F(1) segregating progenies were evaluated through high-resolution gas chromatography-mass spectrometry (HRGC-MS) for the amounts of individual monoterpenes over 3 and 2 years. In the Italia x Big Perlon cross 34 CGs, chosen according to gene ontology (GO) terms, were placed on a complete map and tested for linkage with QTLs for linalool, nerol and geraniol levels. Two CGs mapped within a QTL for linalool content on LG 10. A third one co-localized with a major QTL for the level of the three monoterpenes on LG 5; this gene encodes 1-deoxy-D: -xylulose 5-phosphate synthase (DXS), which is the first enzyme in the plastidial pathway of terpene biosynthesis. Depending on these findings, we report the first in silico analysis of grapevine DXS genes based on the whole genome sequence. Further research on the functional significance of these associations might help to understand the genetic control of Muscat flavor.
  Theoretical and Applied Genetics 12/2008; 118(4):653-69. · 3.30 Impact Factor
• Source

  Available from: Michela Troggio

  Article: A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses, based on 283 SSR and 501 SNP-based markers.

  Silvia Vezzulli, Michela Troggio, Giuseppina Coppola, Angelica Jermakow, Dustin Cartwright, Andrey Zharkikh, Marco Stefanini, M Stella Grando, Roberto Viola, Anne-Françoise Adam-Blondon, Mark Thomas, Patrice This, Riccardo Velasco
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  ABSTRACT: We have developed an integrated map from five elite cultivars of Vitis vinifera L.; Syrah, Pinot Noir, Grenache, Cabernet Sauvignon and Riesling which are parents of three segregating populations. A new source of markers, SNPs, identified in ESTs and unique BAC-end sequences was added to the available IGGP reference set of SSRs. The complete integrated map comprises 1,134 markers (350 AFLP, 332 BESs, 169 ESTs, 283 SSRs) spanning 1,443 cM over 19 linkage groups and shows a mean distance between neighbouring loci of 1.27 cM. Marker order was mainly conserved between the integrated map and the highly dense SyrahxPinot Noir consensus map except for few inversions. Moreover, the marker order has been validated through the assembled genome sequence of Pinot Noir. We have also assessed the transferability of SNP-based markers among five V. vinifera varieties, enabling marker validation across different genotypes. This integrated map can serve as a fundamental tool for molecular breeding in V. vinifera and related species and provide a basis for studies of genome organization and evolution in grapevines.
  Theoretical and Applied Genetics 06/2008; 117(4):499-511. · 3.30 Impact Factor
• Source

  Available from: Michela Troggio

  Article: A grapevine (Vitis vinifera L.) genetic map integrating the position of 139 expressed genes.

  Marzia Salmaso, Giulia Malacarne, Michela Troggio, Giorgia Faes, Marco Stefanini, M Stella Grando, Riccardo Velasco
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  ABSTRACT: Grapevine molecular maps based on microsatellites, AFLP and RAPD markers are now available. SSRs are essential to allow cross-talks between maps, thus upgrading any growing grapevine maps. In this work, single nucleotide polymorphisms (SNPs) were developed from coding sequences and from unique BAC-end sequences, and nested in a SSR framework map of grapevine. Genes participating to flavonoids metabolism and defence, and signal transduction pathways related genes were also considered. Primer pairs for 351 loci were developed from ESTs present on public databases and screened for polymorphism in the "Merzling" (a complex genotype Freiburg 993-60 derived from multiple crosses also involving wild Vitis species) x Vitis vinifera (cv. Teroldego) cross population. In total 138 SNPs, 108 SSR markers and a phenotypic trait (berry colour) were mapped in 19 major linkage groups of the consensus map. In specific cases, ESTs with putatively related functions mapped near QTLs previously identified for resistance and berry ripening. Genes related to anthocyanin metabolism mapped in different linkage groups. A myb gene, which has been correlated with anthocyanin biosynthesis, cosegregated with berry colour on linkage group 2. The possibility of associating candidate genes to known position of QTL is discussed for this plant.
  Theoretical and Applied Genetics 04/2008; 116(8):1129-43. · 3.30 Impact Factor