Jarno Salonen

Harvard University, Cambridge, Massachusetts, United States

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Publications (121)530.06 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Limestones and their modifications from Nordkalk Corporation (Finland) flotation fines (FF) and filter sand (FS) as potential adsorbents for AMD treatment and wastewater purification from Cu, Fe, Zn and Ni ions were studied. Limestones were capable of binding significant amounts of Cu and Fe from synthetic AMD solutions and wastewater, while unmodified limestones were not good for Zn and Ni removal. Two methods of surface area modification were suggested. The first one with 2 M solution of NaCl and the second one with wastewater from Norilsk Nickel Harjavalta. The structure of materials and their surface area were characterized by SEM, EDX, MIR spectroscopy and nitrogen adsorption methods. Optimal amount of adsorbents for different model and real solutions was found. Adsorption kinetics showed that the adsorption equilibrium was reached within approximately 8 h. The kinetic data fits to a pseudo second order model with correlation coefficients greater than 0.999. The adsorption capacity was the highest at solution pH range of 6–7. Langmuir, Toth and Sips models were used to fit the adsorption isotherms. Based on the parameters calculated from models, the adsorption capacity decreased in the order of Cu > Fe > Zn > Ni for FF and Fe > Cu > Zn > Ni for FS. The research showed that the proposed modified limestones can be successfully used for AMD neutralization and removal of Cu(II), Fe(III), Zn(II) and Ni(II).
    Ecological Engineering 08/2015; 81:30-40. · 3.04 Impact Factor
  • Matti Murtomaa, Janne Peltonen, Jarno Salonen
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    ABSTRACT: A new one-step technique to measure the effect of ambient humidity on powder resistivity has been previously presented. In this article, we provide more experimental data obtained with five different powders. One-step measurements and traditional multi-step measurements were performed. Also, additional measurements were performed using standard resistivity cell. Results were compared and it could be concluded that the new technique provided meaningful results although significant hysteresis was observed during humidification and drying cycles. Finally, charging of the powder was also measured and it was noticed that it decreases with decreasing resistivity and increasing humidity.
    Journal of Electrostatics 05/2015; DOI:10.1016/j.elstat.2015.05.016 · 1.27 Impact Factor
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    ABSTRACT: Combination therapy via nanoparticulate systems has already been proposed as a synergistic approach for cancer treatment. Herein, undecylenic acid modified thermally hydrocarbonized porous silicon nanoparticles (UnTHCPSi NPs) loaded with sorafenib and surface-biofunctionalized with anti-CD326 antibody (Ab) were developed for cancer chemo-immunotherapy in MCF-7 and MDA-MB-231 breast cancer cells. The cytocompatibility study showed no significant toxicity for the bare and antibody-conjugated UnTHCPSi (Un-Ab) NPs at concentrations lower than 200 μg·mL−1. Compared to the bare UnTHCPSi, Un-Ab NPs loaded with sorafenib reduced the premature drug release in plasma, increasing the probability of proper drug targeting. In addition, high cellular interaction and subsequent internalization of the Un-Ab NPs into the cells expressing CD326 antigen demonstrated the possibility of improving antigen-mediated endocytosis via CD326 targeting. While an in vitro antitumor study revealed a higher inhibitory effect of the sorafenib-loaded Un-Ab NPs compared to the drug-loaded UnTHCPSi NPs in the CD326 positive MCF-7 cells, there was no difference in the anti-proliferation impact of both the abovementioned NPs in the CD326 negative MDA-MB-231 cells, suggesting CD326 as an appropriate receptor for Ab-mediated drug delivery. It was also shown that the anti-CD326 Ab can act as an immunotherapeutic agent by inducing antibody dependent cellular cytotoxicity and enhancing the interaction of effector immune and cancer cells for subsequent phagocytosis and cytokine secretion. Hence, the developed nanovectors can be applied for simultaneous tumor-selective drug targeting and immunotherapy.
    Nano Research 05/2015; 8(5):1505-1521. DOI:10.1007/s12274-014-0635-4 · 6.96 Impact Factor
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    ABSTRACT: Biocompatible, multifunctional, stimuli-responsive and high drug loading capacity are key factors for the new generation of drug delivery platforms. However, it is extremely challenging to create such a platform that inherits all these advanced properties in a single carrier. Herein, porous silicon nanoparticles (PSi NPs) and giant liposomes were assembled on a microfluidic chip as an advanced nano-in-micro platform (PSi NPs@giant liposomes), which can co-load and co-deliver hydrophilic and hydrophobic drugs combined with synthesized DNA nanostructures, short gold nanorods and magnetic nanoparticles. The PSi NPs@giant liposomes with photothermal and magnetic responsiveness showed good biocompatibility, high loading capacity and controllable release. The hydrophilic thermal oxidized PSi NPs encapsulate hydrophobic therapeutics within the hydrophilic core of the giant liposomes, endowing high therapeutics loading capacity with tuneable ratio and controllable release. We demonstrate that the DAO-E A DNA nanostructures have synergism with drugs and importantly they contribute to the significant enhancement of cell death to doxorubicin-resistant MCF-7/DOX cells, overcoming the multidrug resistance in the cancer cells. Therefore, the PSi NPs@giant liposomes nano-in-micro platform hold great potential for a cocktail delivery of drugs and DNA nanostructures for effective cancer therapy, controllable drug release with tuneable therapeutics ratio, and both photothermal and magnetic dual-responsiveness.
    Advanced Functional Materials 04/2015; DOI:10.1002/adfm.201500594 · 10.44 Impact Factor
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    ABSTRACT: A hybrid nanocomposite comprised by porous silicon nanoparticles and a stimuli responsive polymeric material, polyethylene glycol-block-poly(L-histidine), is spontaneously formed by nanoprecipitation in a flow-focusing microfluidic chip. The nanocomposite presents a novel hybrid compound micelle structure with a great robustness for therapeutic applications. Therefore, the nanocomposite is developed and tested as a “smart” multistage drug delivery system (MDDS) in response to some of the current problems that cancer treatment presents. Based on the stimuli-responsive behavior of the nanocomposite, a chemotherapeutic agent is successfully loaded into the nanosystem and released upon changes in the pH-values. The nanocomposite demonstrates enhanced stability in plasma, narrow size distribution, improved surface smoothness, and high cytocompatibility. Furthermore, the nanocomposite presents reduced nanoparticle internalization by phagocytic macrophage cells and pH-dependent cell growth inhibition capacity. Overall, the developed hybrid nanocomposite shows very promising features for its further development as a “smart” pH-responsive MDDS.
    Advanced Functional Materials 03/2015; 25(10):1488–1497. DOI:10.1002/adfm.201404122 · 10.44 Impact Factor
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    ABSTRACT: A hybrid nanocomposite comprised by porous silicon nanoparticles and a stimuli responsive polymeric material, polyethylene glycol-block-poly(L-histidine), is spontaneously formed by nanoprecipitation in a flow-focusing microfluidic chip. The nanocomposite presents a novel hybrid compound micelle structure with a great robustness for therapeutic applications. Therefore, the nanocomposite is developed and tested as a “smart” multistage drug delivery system (MDDS) in response to some of the current problems that cancer treatment presents. Based on the stimuli-responsive behavior of the nanocomposite, a chemotherapeutic agent is successfully loaded into the nanosystem and released upon changes in the pH-values. The nanocomposite demonstrates enhanced stability in plasma, narrow size distribution, improved surface smoothness, and high cytocompatibility. Furthermore, the nanocomposite presents reduced nanoparticle internalization by phagocytic macrophage cells and pH-dependent cell growth inhibition capacity. Overall, the developed hybrid nanocomposite shows very promising features for its further development as a “smart” pH-responsive MDDS.
    Advanced Functional Materials 03/2015; 25(10):1612. DOI:10.1002/adfm.201570073 · 10.44 Impact Factor
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    ABSTRACT: Nanomaterials provide a unique platform for the development of theranostic systems that combine diagnostic imaging modalities with a therapeutic payload in a single probe. In this work, dual-labeled iRGD-modified multifunctional porous silicon nanoparticles (PSi NPs) were prepared from dibenzocyclooctyl (DBCO) modified PSi NPs by strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrophobic antiangiogenic drug, sorafenib, was loaded into the modified PSi NPs to enhance the drug dissolution rate and improve cancer therapy. Radiolabeling of the developed system with (111)In enabled the monitoring of the in vivo biodistribution of the nanocarrier by single photon emission computed tomography (SPECT) in an ectopic PC3-MM2 mouse xenograft model. Fluorescent labeling with Alexa Fluor 488 was used to determine the long-term biodistribution of the nanocarrier by immunofluorescence at the tissue level ex vivo. Modification of the PSi NPs with an iRGD peptide enhanced the tumor uptake of the NPs when administered intravenously. After intratumoral delivery the NPs were retained in the tumor, resulting in efficient tumor growth suppression with particle-loaded sorafenib compared to the free drug. The presented multifunctional PSi NPs highlight the utility of constructing a theranostic nanosystems for simultaneous investigations of the in vivo behavior of the nanocarriers and their drug delivery efficiency, facilitating the selection of the most promising materials for further NP development. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Biomaterials 02/2015; 48:108-118. DOI:10.1016/j.biomaterials.2015.01.008 · 8.31 Impact Factor
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    ABSTRACT: Dual-drug delivery of antiangiogenic drug and chemotherapeutic drug can enhance the therapeutic effect for cancer therapy. Conjugation of methotrexate (MTX) to PSi nanoparticles (MTX-PSi) with positively charged surfaces can improve the cellular uptake of MTX and inhibit the proliferation of cancer cells. Herein, MTX-PSi conjugates sustained the release of MTX up to 96h, and the released fragments including MTX were confirmed by mass spectrometry. The intracellular distribution of the MTX-PSi nanoparticles was confirmed by transmission electronic microscopy. Compared to pure MTX, the MTX-PSi achieved similar inhibition of cell proliferation in folate receptor (FR) over-expressing U87 MG cancer cells, and a higher effect in low FR-expressing EA.hy926 cells. Nuclear fragmentation analysis demonstrated programed cell apoptosis of the MTX-PSi in the high/low FR-expressing cancer cells, whereas PSi alone at the same dose had a minor effect on cell apoptosis. Finally, the porous structure of MTX-PSi enabled a successful concomitant loading of another anti-angiogenic hydrophobic drug, sorafenib, and considerably enhanced the dissolution rate of sorafenib. Overall, the MTX-PSi nanoparticle system can be used as a platform for combination chemotherapy by enhancing the dissolution rate of the hydrophobic drug and sustaining the release of the conjugated chemotherapeutic drug. Copyright © 2015. Published by Elsevier Ltd.
    Acta Biomaterialia 01/2015; 16. DOI:10.1016/j.actbio.2015.01.021 · 5.68 Impact Factor
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    ABSTRACT: Porous silicon (PSi) nanoparticles' tunable properties are facilitating their use at highly challenging medical tasks such as peptide delivery. Due to many different mechanisms that are affecting the interaction between the peptide and the particle, the drug incorporation into the mesoporous delivery system is not straightforward. We have studied the adsorption and loading of incretin hormone glucagon like peptide 1 (GLP-1) on PSi nanoparticles. The results show that the highest loading degree can be achieved in pH values near the isoelectric point of peptide and the phenomenon is independent on the surface's zeta potential. In order to study the interaction between the peptide and the nanoparticle, we studied the adsorption with lower concentrations and noticed that also non-coulombic forces have a big role on adsorption of GLP-1. Adsorption is effective and pH-independent especially on low peptide concentrations and onto more hydrophobic nanoparticles. Reversibility of adsorption was studied as a function of buffer pH.. When the loading is compared to the total mass of the formulation, the loading degree is 29 % and during desorption experiments 25 % is released in four hours and can be considered as a reversible loading degree. Thus the peptides adsorbed first seem to create irreversibly adsorbed layer that facilitates reversible adsorption of following peptides.
    Langmuir 01/2015; DOI:10.1021/la5047047 · 4.38 Impact Factor
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    ABSTRACT: The main goal of the current work was to investigate the possible use of flexographic printing for the conversion of nanosuspensions into solid dosage forms. Aqueous nanosuspensions of indomethacin (IND) and itraconazole (ITR) with Poloxamer 407 as the stabilizer agent were prepared by wet ball-milling. The nanosuspensions were flexographically printed on three different substrates, including two commercially available edible substrates. The printed formulations were characterized with X-ray diffractometry (XRD) and scanning electron microscopy (SEM). In addition, dissolution studies for the printed IND and ITR formulations were conducted. The mean particle size of milled nanosuspensions of IND and ITR was 422.6±7.7nm and 698.1±14.0nm, respectively. The SEM imaging showed even distribution of nanosuspensions on the substrates after printing without any evident agglomeration. The printed formulations contained drug at least partially in crystalline form. The drug dissolution rate from the prepared formulations was improved compared to the pure drug. The drug release from the preparations on edible substrates was slightly slower due to the incorporation of the drug particles into the substrate matrix. In conclusion, the results indicated that flexographic printing can be considered as a promising fabrication method of solid nanoparticulate systems with enhanced dissolution behavior. Copyright © 2015. Published by Elsevier B.V.
    International Journal of Pharmaceutics 01/2015; DOI:10.1016/j.ijpharm.2015.01.027 · 3.79 Impact Factor
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    ABSTRACT: Porous silicon (PSi) nanomaterials combine a high drug loading capacity and tunable surface chemistry with various surface modifications to meet the requirements for biomedical applications. In this work, alkyne-terminated thermally hydrocarbonized porous silicon (THCPSi) nanoparticles were fabricated and postmodified using five bioactive molecules (targeting peptides and antifouling polymers) via a single-step click chemistry to modulate the bioactivity of the THCPSi nanoparticles, such as enhancing the cellular uptake and reducing the plasma protein association. The size of the nanoparticles after modification was increased from 176 to 180–220 nm. Dextran 40 kDa modified THCPSi nanoparticles showed the highest stability in aqueous buffer. Both peptide- and polymer-functionalized THCPSi nanoparticles showed an extensive cellular uptake which was dependent on the functionalized moieties presented on the surface of the nanoparticles. The plasma protein adsorption study showed that the surface modification with different peptides or polymers induced different protein association profiles. Dextran 40 kDa functionalized THCPSi nanoparticles presented the least protein association. Overall, these results demonstrate that the “click” conjugation of the biomolecules onto the alkyne-terminated THCPSi nanoparticles is a versatile and simple approach to modulate the surface chemistry, which has high potential for biomedical applications.
    ACS Applied Materials & Interfaces 01/2015; 7(3):2006-2015. · 5.90 Impact Factor
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    ABSTRACT: An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach. The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility. Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control. The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix. To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry. Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites. Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy. Copyright © 2014 Elsevier Ltd. All rights reserved.
    Biomaterials 01/2015; 39(42):249–259. DOI:10.1016/j.biomaterials.2014.10.079 · 8.31 Impact Factor
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    ABSTRACT: A roll-to-roll compatible fabrication process of porous silicon (pSi) based sensing elements for a real-time humidity monitoring is described. The sensing elements, consisting of printed interdigitated silver electrodes and a spray-coated pSi layer, were fabricated on a coated paper substrate by a two-step process. Capacitive and resistive responses of the sensing elements were examined under different concentrations of humidity. More than a three orders of magnitude reproducible decrease in resistance was measured when the relative humidity (RH) was increased from 0% to 90%. A relatively fast recovery without the need of any refreshing methods was observed with a change in RH. Humidity background signal and hysteresis arising from the paper substrate were dependent on the thickness of sensing pSi layer. Hysteresis in most optimal sensing element setup (a thick pSi layer) was still noticeable but not detrimental for the sensing. In addition to electrical characterization of sensing elements, thermal degradation and moisture adsorption properties of the paper substrate were examined in connection to the fabrication process of the silver electrodes and the moisture sensitivity of the paper. The results pave the way towards the development of low-cost humidity sensors which could be utilized, for example, in smart packaging applications or in smart cities to monitor the environment.
    Journal of Sensors 01/2015; 2015(Article ID 927396):10 pages. DOI:10.1155/2015/927396
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    ABSTRACT: Porous silicon nanoparticles (pSiNPs) are a promising nanocarrier system for drug delivery owing to their biocompatibility, biodegradability, and non-inflammatory nature. Here, we investigate the fabrication and characterization of thermally hydrocarbonized pSiNPs (THCpSiNPs) and chitosan-coated THCpSiNPs for therapeutic oligonucleotide delivery. Chitosan coating after oligonucleotide loading significantly improves sustained oligonucleotide release and suppresses burst release effects. Moreover, cellular uptake, endocytosis, and cytotoxicity of oligonucleotide-loaded THCpSiNPs have been evaluated in vitro. Standard cell viability assays demonstrate that cells incubated with the NPs at a concentration of 0.1 mg/mL are 95% viable. In addition, chitosan coating significantly enhances the uptake of oligonucleotide-loaded THCpSiNPs across the cell membrane. Moreover, histopathological analysis of liver, kidney, spleen, and skin tissue collected from mice receiving NPs further demonstrates the biocompatible and non-inflammatory properties of the NPs as a gene delivery vehicle for intravenous and subcutaneous administration in vivo. Taken together, these results suggest that THCpSiNPs provide a versatile platform that could be used as efficient vehicles for the intracellular delivery of oligonucleotides for gene therapy.
    Nano Research 01/2015; DOI:10.1007/s12274-015-0715-0 · 6.96 Impact Factor
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    ABSTRACT: Two limestones and two solid wastes were tested as adsorbents for chloride and sulphate ion removal from synthetic and real alkaline process mining water. The composition and surface structure of the adsorbents were analysed by ED-XRF, XRD, SEM and MIR spectroscopy and the nitrogen adsorption method. The optimal adsorption time and amount of adsorbent were determined by batch analysis with an anion concentration of 20 mg/L. In synthetic solution adsorbents removed 99% of SO42− and 96% of Cl− and in real process water the removal rate was 85% and 74% for SO42− and Cl−, respectively. It was found that equilibrium could successfully be fitted to the Langmuir, Toth and BiLangmuir adsorption isotherms.
    Chemical Engineering Journal 01/2015; 259:364–371. DOI:10.1016/j.cej.2014.07.091 · 4.06 Impact Factor
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    ABSTRACT: Abstract Context: Electrospraying was used in drug particle production. Objective: The aim of the research was to evaluate the possibilities to produce drug particles with desired pharmaceutical properties by electrospraying. In particular, the effect of drying pressure on particle properties was studied. Materials and methods: A poorly water soluble model drug (budesonide) was dissolved in chloroform, and the solution was atomized by electrospraying. Following this, the charged droplets were neutralized and dried in a drying chamber. The pressure in the drying chamber was varied. The dried particles were collected and analyzed. Results: The pressure reduction had a slight impact on particle size distribution. The particles produced in reduced pressure turned out to be notably more porous than the particles produced in atmospheric pressure. The pressure reduction also affects the degree of crystallinity of the product. The dissolution of the particles produced in reduced pressures was faster to a certain extent than that of the particles produced in atmospheric pressure. Discussion and conclusions: A setup for electrospraying materials in a reduced pressure was presented. The pressure reduction had a notable impact on particle morphology. The possibilities to tailor the particle properties during electrospraying were studied.
    Drug Development and Industrial Pharmacy 01/2015; 41(1):116-123. DOI:10.3109/03639045.2013.850708 · 2.01 Impact Factor
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    ABSTRACT: The aim of the research was to verify that electrospraying of piroxicam yielded a new polymorphic form of this drug. In the experiments, piroxicam was dissolved in chloroform and the solution was atomised electrostatically. Subsequently, the charged droplets were neutralised and dried. The solid drug particles were collected and analysed by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, high performance liquid chromatography, and infrared and Raman spectroscopy. The X-ray diffractogram measured for the electrosprayed piroxicam particles did not match with any of the known piroxicam crystal structures (Cambridge Crystallographic Data Centre). The variable temperature X-ray diffraction showed that the structure recrystallised completely into piroxicam polymorphic formI during heating. No degradation products or solvate removal were detected by high performance liquid chromatography and thermal analysis. The infrared and Raman spectra of the electrosprayed piroxicam were compared to those of formI, and some notable differences in the peak positions, shapes and intensities were detected. The results indicate that electrospraying leads to piroxicam crystallisation in a currently unknown polymorphic form. Copyright © 2014. Published by Elsevier B.V.
    European Journal of Pharmaceutics and Biopharmaceutics 01/2015; 81(1):182–189. DOI:10.1016/j.ejpb.2014.11.027 · 4.25 Impact Factor
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    ABSTRACT: Given the established role of Chlamydia spp. as causative agents of both acute and chronic diseases, search for new antimicrobial agents against these intracellular bacteria is required to promote human health. Isoflavones are naturally occurring phytoestrogens, antioxidants and efflux pump inhibitors, but their therapeutic use is limited by poor water-solubility and intense first-pass metabolism. Here, we report on effects of isoflavones against C. pneumoniae and C. trachomatis and describe buccal permeability and initial formulation development for biochanin A. Biochanin A was the most potent Chlamydia growth inhibitor among the studied isoflavones, with an IC50 = 12 µM on C. pneumoniae inclusion counts and 6.5 µM on infectious progeny production, both determined by immunofluorescent staining of infected epithelial cell cultures. Encouraged by the permeation of biochanin A across porcine buccal mucosa without detectable metabolism, oromucosal film formulations were designed and prepared by a solvent casting method. The film formulations showed improved dissolution rate of biochanin A compared to powder or a physical mixture, presumably due to the solubilizing effect of hydrophilic additives and presence of biochanin A in amorphous state. In summary, biochanin A is a potent inhibitor of Chlamydia spp., and the in vitro dissolution results support the use of a buccal formulation to potentially improve its bioavailability in antichlamydial or other pharmaceutical applications.
    PLoS ONE 12/2014; 9(12):e115115. DOI:10.1371/journal.pone.0115115 · 3.53 Impact Factor
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    ABSTRACT: This study reports the use of variants of coherent anti-Stokes Raman scattering (CARS) microscopy as a novel method for improved physicochemical characterization of drug loaded silica particles. Ordered mesoporous silica is a biomaterial that can be loaded to carry a number of biochemicals including poorly water-soluble drugs by allowing the incorporation of drug into nanometer-sized pores. In this work, we qualitatively characterized the loading of two poorly water-soluble model drugs, itraconazole and griseofulvin, in MCM-41 silica microparticles using the novel approach of CARS microscopy, which has advantages over other analytical approaches used to date and is nondestructive, rapid, label-free, confocal and has chemical and physical specificity. We investigated the effect of two solvent-based loading methods, namely immersion and rotary evaporation, as well as microparticle size, on the three dimensional (3D) distribution of the two loaded drugs. Additionally, we used hyperspectral CARS microscopy to confirm the amorphous nature of the loaded drugs. Z-stacked CARS microscopy suggested the drug, but not loading method or particle size range, affected the 3D drug distribution. Hyperspectral CARS confirmed that the drug loaded in the MCM-41 silica microparticles was in an amorphous form. Our results show that CARS microscopy and hyperspectral CARS microscopy can be used to provide further insights into the structural nature of loaded mesoporous silica microparticles as biomaterials.
    Acta Biomaterialia 11/2014; 10(11):4870–4877. DOI:10.1016/j.actbio.2014.07.021 · 5.68 Impact Factor
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    ABSTRACT: The wound healing stands as very complex and dynamic process, aiming the re-establishment of the damaged tissue's integrity and functionality. Thus, there is the emerging need for developing biopolymer-based composites capable of actively promote cellular proliferation and reconstitute the extracellular matrix. The aims of the present work were to prepare and characterize biopolymer-functionalized porous silicon (PSi) microparticles, resulting in the development of drug delivery microsystems for future applications in wound healing. Thermally hydrocarbonized PSi (THCPSi) microparticles were coated with both chitosan (CHI) and a mixture of chondroitin sulfate/hyaluronic acid (CS/HA), and subsequently loaded with two antibacterial model drugs, vancomycin and resveratrol. The biopolymer coating, drug loading degree and drug release behaviour of the modified PSi microparticles were evaluated in vitro. The results showed that both the biopolymer coating and drug loading of the THCPSi microparticles were successfully achieved. In addition, a sustained release was observed for both the drugs tested. The viability and proliferation profiles of a fibroblast cell line exposed to the modified THCPSi microparticles and the subsequent reactive oxygen species (ROS) production were also evaluated. The cytotoxicity and proliferation results demonstrated less toxicity for the biopolymer-coated THCPSi microparticles at different concentrations and time points comparatively to the uncoated counterparts. The ROS production by the fibroblasts exposed to both uncoated and biopolymer-coated PSi microparticles showed that the modified PSi microparticles did not induce significant ROS production at the concentrations tested. Overall, the biopolymer-based PSi microparticles developed in this study are promising platforms for wound healing applications.
    European Journal of Pharmaceutics and Biopharmaceutics 10/2014; 88(3):635–642. DOI:10.1016/j.ejpb.2014.09.010 · 4.25 Impact Factor

Publication Stats

2k Citations
530.06 Total Impact Points


  • 2015
    • Harvard University
      • Department of Physics
      Cambridge, Massachusetts, United States
  • 2004–2015
    • University of Turku
      • • Department of Physics and Astronomy
      • • Department of Physiatrics
      Turku, Province of Western Finland, Finland
  • 2014
    • Hospital Regional Universitario de Málaga
      Málaga, Andalusia, Spain
  • 2011
    • University of Helsinki
      • Division of Pharmaceutical Technology
      Helsinki, Province of Southern Finland, Finland