Peter Lindberg

Publications (9)30.69 Total impact

• Article: Temporal trends of polybrominated diphenyl ethers and hexabromocyclododecane in Swedish Peregrine Falcon (Falco peregrinus peregrinus) eggs.

  Anna-Karin Johansson, Ulla Sellström, Peter Lindberg, Anders Bignert, Cynthia A de Wit
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  ABSTRACT: A temporal trend study of brominated flame retardants in eggs from peregrine falcon (Falco peregrinus peregrinus), a terrestrial bird of prey, is presented. Eggs collected between 1974 and 2007 were analyzed for the major constituents of the Penta-, Octa- and Decabromodiphenyl ether technical products (BDE-47, -99, -100, -153, -183 and -209), and hexabromocyclododecane (HBCD). Concentrations of BDE-99, -100, -153, -183, -209 and HBCD increased from 1974 to 2000. After the early 2000s, BDE-99, -100, -153 and -183 concentrations decreased, whereas BDE-209 and HBCD concentrations continued to increase. No temporal trend was detected for BDE-47. Rates of increase also differed, with BDE-99 and -100 increasing 3-fold between the 1980s and mid-1990s, and BDE-153 and -183 increasing approximately 10-fold during the same period. The average yearly increase was 15% and 11% for BDE-209 and HBCD, respectively, based on log-linear regression trends. There is a change in BDE congener patterns over time, with a shift from the predominance of BDE-99 and -47 until the late 1980s, to BDE-153 becoming the predominant congener later on. BFR temporal trends in Swedish peregrine falcon eggs reflect European BFR usage patterns.
  Environment international 03/2011; 37(4):678-86. · 4.79 Impact Factor
• Article: Temporal trends of perfluorinated surfactants in Swedish peregrine falcon eggs (Falco peregrinus), 1974-2007.

  Katrin E Holmström, Anna-Karin Johansson, Anders Bignert, Peter Lindberg, Urs Berger
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  ABSTRACT: Perfluorinated alkyl substances (PFAS) are today known to be globally distributed environmental contaminants. In the present study, concentrations of PFAS were analyzed in Swedish peregrine falcon eggs (Falco peregrinus), collected between 1974 and 2007. Analytes included in the study were perfluorinated carboxylates (PFCAs; carbon chain lengths C6-C15), perfluorinated sulfonates (PFSAs; C4, C6, C8, and C10), and perfluorooctane sulfonamide (PFOSA). The predominant PFAS was perfluorooctane sulfonate, PFOS (83 ng/g wet weight (w wt) mean concentration in samples from 2006), followed by perfluorotridecanoate, PFTriA (7.2 ng/g w wt) and perfluoroundecanoate, PFUnA (4.2 ng/g w wt). PFCA concentrations increased exponentially over the studied time. In contrast, concentrations of PFOS and perfluorohexane sulfonate (PFHxS) increased initially but leveled off after the mid 1980s. This is different from previously observed temporal trends in marine organisms. The present study is the first to establish temporal trends for PFAS in terrestrial biota. The results indicate potential differences between marine and terrestrial biota regarding sources of PFAS exposure and response to emission changes. The toxicological implications of PFAS exposure for the falcons are not known, but according to recent findings impaired hatching success and sublethal toxicological effects from PFOS exposure in the Swedish peregrine falcon cannot be ruled out.
  Environmental Science and Technology 06/2010; 44(11):4083-8. · 5.23 Impact Factor
• Article: Polybrominated diphenyl ether congener patterns, hexabromocyclododecane, and brominated biphenyl 153 in eggs of peregrine falcons (Falco peregrinus) breeding in Sweden

  Anna-Karin Johansson, Ulla Sellström, Peter Lindberg, Anders Bignert, Cynthia A. de Wit
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  ABSTRACT: Previous analyses of 52 peregrine falcon (Falco peregrinus) eggs collected from two wild and one captive population in Sweden 1987 through 1999 were complemented by including additional polybrominated diphenyl ether (PBDE) congeners (BDE −35, −183, −184, −185, −196, −197, −;203, and −207). In addition, 31 eggs not previously analyzed for hexabromocyclododecane (HBCD) and BDE-209 were analyzed for these. Geometric mean concentrations of ΣPBDEs, HBCD, and the hexabrominated biphenyl (BB-153) were 3,100, 140, and 81 ng/g of lipid weight for the southern population; 2,500, 110, and 84 ng/g of lipid weight for the northern population; and 47, not detected, and 8 ng/g of lipid weight for the captive population. The BDE congener pattern was dominated by BDE-153, −99, and −100. The results were used to investigate whether a difference in PBDE congener pattern could be distinguished between the two wild populations of peregrine falcons due to different diets, as the southern population preys mainly on birds belonging to the terrestrial food chain while the northern population preys more on aquatic birds. A multivariate t-test showed a subtle but significant (p < 0.001) difference in PBDE congener pattern between the two populations. However, our hypothesis that higher-brominated congeners of PBDEs would be present to a greater extent in the terrestrial food chain was not supported by principal component analysis. The average brood size for individual females from the southern population decreased with increasing concentrations of ΣPBDE in the eggs (log-linear regressionp < 0.01).
  Environmental Toxicology and Chemistry 12/2008; 28(1):9 - 17. · 2.81 Impact Factor
• Article: Separation of oligonucleotides in N-methyl-formamide-based polymer matrices by capillary electrophoresis.

  Johan Sjödahl, Peter Lindberg, Johan Roeraade
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  ABSTRACT: N-Methylformamide (NMF)-based matrices for capillary electrophoretic separation of nucleic acids have been developed. The use of an organic solvent as liquid base for the separation matrices allowed a hydrophobic polymer, C16-derivatized 2-hydroxyethyl cellulose (HEC), to be employed as structural element in the sieving medium. With a matrix consisting of 5% w/v of this polymer dissolved in NMF containing 50 mM ammonium acetate, p(dA)12-18 and p(dA)40-60 oligonucleotides were baseline separated. The addition of ammonium acetate to the buffer and separation matrix resulted in enhanced separation efficiency. Furthermore, it was possible to tailor the sieving performance of the separation medium by the use of a binary mixture of C16-derivatized HEC and PVP. Differences in sieving behavior of the various matrices evaluated are discussed.
  Journal of Separation Science 02/2007; 30(1):104-9. · 2.73 Impact Factor
• Source

  Available from: Per E Andrén

  Article: Sample pretreatment on a microchip with an integrated electrospray emitter

  Peter Lindberg, Andreas P. Dahlin, Sara K. Bergström, Sara Thorslund, Per E. Andrén, Fredrik Nikolajeff, Jonas Bergquist Professor
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  ABSTRACT: This study presents a microbead-packed PDMS microchip with an integrated electrospray emitter for sample pretreatment prior to sheathless ESI-MS. We prove the concept of analytical functions integrated onto a cm-sized area of a single bulk material. The microchip consists of two PDMS substrates replicated from SU-8 fabricated silicon wafer masters, bonded together after oxidation by corona discharge treatment. The channel within the microchip contains a grid structure that was used to trap 5 µm hypercross-linked polystyrene beads. The beads acted as a medium for sample desalting and enrichment. Electrical contact for the sheathless ESI process was achieved by coating the integrated emitter with conductive graphite powder after applying a thin layer of PDMS as glue. The coating as well as the bond of the PDMS structures showed excellent durability. A continuous spray was obtained from the microchip for over 800 h in a long-term electrospray stability experiment. Desalting and enrichment of neuropeptides from a physiological salt solution was successful by loading the sample onto the packed beads, followed by a washing and an eluting step. The results were obtained and evaluated using a TOF MS. An LOD of approximately 20 fmol (loaded onto the beads) for angiotensin II was obtained from a sample of neuropeptides dissolved in physiological salt solution.
  Electrophoresis 04/2006; 27(11):2075 - 2082. · 3.30 Impact Factor
• Source

  Available from: uu.se

  Article: Electrokinetic‐driven microfluidic system in poly(dimethylsiloxane) for mass spectrometry detection integrating sample injection, capillary electrophoresis, and electrospray emitter on‐chip

  Sara Thorslund, Peter Lindberg, Per E. Andrén, Fredrik Nikolajeff, Jonas Bergquist Professor, Jonas Bergquist
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  ABSTRACT: A novel microsystem device in poly(dimethylsiloxane) (PDMS) for MS detection is presented. The microchip integrates sample injection, capillary electrophoretic separation, and electrospray emitter in a single substrate, and all modules are fabricated in the PDMS bulk material. The injection and separation flow is driven electrokinetically and the total amount of external equipment needed consists of a three-channel high-voltage power supply. The instant switching between sample injection and separation is performed through a series of low-cost relays, limiting the separation field strength to a maximum of 270 V/cm. We show that this set-up is sufficient to accomplish electrospray MS analysis and, to a moderate extent, microchip separation of standard peptides. A new method of instant in-channel oxidation makes it possible to overcome the problem of irreversibly bonded PDMS channels that have recovered their hydrophobic properties over time. The fast method turns the channel surfaces hydrophilic and less prone to nonspecific analyte adsorption, yielding better separation efficiencies and higher apparent peptide mobilities.
  Electrophoresis 11/2005; 26(24):4674 - 4683. · 3.30 Impact Factor
• Source

  Available from: uni.hu

  Article: Higher brominated diphenyl ethers and hexabromocyclododecane found in eggs of peregrine falcons (Falco peregrinus) breeding in Sweden.

  Peter Lindberg, Ulla Sellström, Lisbeth Häggberg, Cynthia A de Wit
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  ABSTRACT: Several brominated flame retardants (BFRs) were analyzed in peregrine falcon eggs collected in 1987-1999, including the constituents of the technical polybrominated diphenyl ether (PBDE) products Penta (BDE-47, -99, -100, -153, -154), Octa (BDE-183), and Deca (BDE-209), hexabrominated biphenyl (BB-153), and hexabromocyclododecane (HBCD). The eggs represented females from three different breeding populations, northern Sweden, southwestern Sweden, and a captive breeding population. All BFRs analyzed for were found, including BDE-183 and -209, and concentrations were much higher in wild falcons (geometric mean sigmaPBDE, BB-153, and HBCD for northern/southern populations of 2200/2700, 82/77, and 150/250 ng/g lw, respectively) than in captive falcons (39, 8 ng/g lw, and not detected, respectively). This is the first time, to our knowledge, that BDE-183 and -209 have been quantified in high trophic level wildlife.
  Environmental Science and Technology 02/2004; 38(1):93-6. · 5.23 Impact Factor
• Article: Multiplex high-throughput solid-phase minisequencing by capillary electrophoresis and liquid core waveguide fluorescence detection.

  Mario Curcio, Per Stålhandske, Peter Lindberg, Johan Roeraade
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  ABSTRACT: Minisequencing, solid-phase single-nucleotide primer extension reaction, is a robust method for performing multiplex single-nucleotide polymorphism (SNP) analysis. We have combined this technology with capillary gel electrophoresis in a multicapillary format, using liquid core waveguide (LCW) fluorescence detection. Polymerase chain reaction (PCR) amplification of multiple DNA targets is performed with one primer for each target biotinylated. Separation of the complementary strands, minisequencing and washing steps are carried out using streptavidin-coated magnetic beads. Dideoxynucleotides analogues labelled with different fluorophores are used for the extension of the minisequencing primers. The extended oligonucleotides, the length of which defines the position on the target and the color the identity of the polymorphism, are then separated in a gel-filled array of capillaries, coated on the outside with a layer of a fluoropolymer to provide the liquid core waveguide characteristics. The technology has a potential for extremely high throughputs when a combination of multiplex PCR-minisequencing is used together with a large array of capillaries, four-color detection and high-speed separation.
  Electrophoresis 06/2002; 23(10):1467-72. · 3.30 Impact Factor
• Chapter: DNA Sequencing at Elevated Temperature by Capillary Electrophoresis

  Peter Lindberg, Johan Roeraade
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  ABSTRACT: The human genome initiative (1-3) has spurred the development of analytical instrumentation for high-throughput DNA sequencing. Originally carried out only in poly(acrylamide) slab gels, DNA sequencing is currently to an increasing extent performed in the capillary electrophoresis (CE) format, utilizing narrow bore columns filled with a polymer sieving matrix (4). The advantages of the latter technique are the analysis speed, the possibility of online detection and quantitation, as well as the ease of automation, whereas the advantage of the former technique is the parallel capacity of the gel slab. In order to obtain parallel capacity also in the CE format, capillary array electrophoresis (CAE) systems have been developed in recent years (5-8), for use in high throughput sequencing applications. Another less obvious feature of the slab-gel systems is that the DNA separation is carried out at temperatures above ambient, due to the heat generated by the relatively large currents (compared to CE) that are present in these systems during electrophoresis (9). Elevated temperature has positive effects on the base calling accuracy, the analysis time, and the amount of DNA sequence data that can be obtained from an electrophoretic separation (10). In CE, the generally low currents generated and the efficient heat dissipation from the column prevents any appreciable temperature rise during the analysis, so an external heating source must be employed. A number of CE setups that have been designed to operate at elevated temperatures are described in this chapter, along with some fundamental information on DNA sequencing, on the principles of CE, as well as on the DNA structure and the formation of hairpin loops. The effects of elevated temperatures on the polymer separation matrix and on the migration of DNA fragments are discussed as well.
  12/2000: pages 289-308;