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Photomedicine and laser surgery 01/2010; 28(1):63-68. · 1.76 Impact Factor
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ABSTRACT: This study investigated and correlated the kinetic expression of vascular endothelial growth factor (VEGF)-A(165) messenger ribonucleic acid (mRNA) with the associated use or not of an infrared laser and a visible red laser during the wound healing in rats.
There is a lack of scientific evidence demonstrating the influence of low-level laser therapy (LLLT) on the expression of VEGF mRNA in vivo.
Forty-five Wistar rats were randomly allocated to one of three groups: I (n = 5, nonoperated animals), II (n = 25, operated animals), and III (n = 25, animals operated and subjected to laser irradiation). A surgical wound was performed using a scalpel in the right side of the tongue of operated animals. In group III, two sessions of laser irradiation were performed, one right after the surgical procedure (infrared laser, 780 nm, 70 mW, 35 J/cm(2)) and the other 48 h later (visible red laser, 660 nm, 40 mW, 5 J/cm(2)). Five animals each were sacrificed 1, 3, 5, and 7 days postoperatively in groups II and III, and samples of tongue tissue were obtained. The animals of group I were sacrificed on day 7. Total RNA was extracted using guanidine-isothiocyanate-phenol-chloroform method. The results of horizontal electrophoresis after reverse transcription polymerase chain reaction permitted the ratio of VEGF-A(165) mRNA and glyceraldehyde 3-phosphate dehydrogenase mRNA expression for groups I, II, and III to be assessed (two-way analysis of variance and Tukey test, p < 0.05).
The expression of VEGF-A(165) mRNA in group II (0.770 +/- 0.098) was statistically greater than that observed in groups I (0.523 +/- 0.164) and III (0.504 +/- 0.069) in the first day after surgery (p < 0.05). Significant differences between the groups were not observed in other time periods.
LLLT influenced the expression of VEGF-A(165) mRNA during wound healing after a surgical procedure on the tongue of Wistar rats.
Photomedicine and laser surgery 09/2009; 28(1):63-8. · 1.76 Impact Factor
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ABSTRACT: Vascular endothelial growth factor (VEGF) is a macromolecule of importance in inflammation that has been implicated in periodontitis. The aims of this study were to investigate VEGF expression during the progression of periodontal disease and to evaluate the effect of a preferential cyclooxygenase (COX)-2 inhibitor meloxicam on VEGF expression and alveolar bone loss in experimentally induced periodontitis.
A total of 120 Wistar rats were randomly separated into groups 1 (control) and 2 (meloxicam, 3 mg/kg/day, intraperitoneally, for 3, 7, 14, or 30 days). Silk ligatures were placed at the gingival margin level of the lower right first molar of all rats. VEGF expression was assessed by reverse transcription-polymerase chain reaction (RT-PCR), Western blot (WB), and immunohistochemical (IHC) analyses. The hemiarcades were processed for histopathologic analysis. RT-PCR and WB results were submitted to analysis of variance, the Tukey test, and Pearson correlation analysis (P <0.05).
A reduction in alveolar bone resorption was observed in the meloxicam-treated group compared to the control group at all periods studied. There was a positive correlation between COX-2 mRNA and VEGF mRNA in the gingival tissues and periodontal disease (R = 0.80; P = 0.026). Meloxicam significantly reduced the increased mRNA VEGF expression in diseased tissues after 14 days of treatment (P = 0.023). Some alterations in VEGF receptor 1 mRNA expression were observed, but these were not statistically significant. VEGF protein expression in WB experiments was significantly higher in diseased sites compared to healthy sites (P <0.05). After 14 days of treatment with meloxicam, an important decrease in VEGF protein expression was detected in diseased tissues (P = 0.08). Qualitative IHC analysis revealed that VEGF protein expression was higher in diseased tissues and decreased in tissues from rats treated with meloxicam.
The present data suggest an important role for VEGF in the progression of periodontal disease. Systemic therapy with meloxicam can modify the progression of experimentally induced periodontitis in rats by reducing VEGF expression and alveolar bone loss.
Journal of Periodontology 07/2008; 79(6):1062-9. · 2.60 Impact Factor
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Leonardo V L Gregorio,
Fernando P M Giglio, Vivien T Sakai,
Karin C S Modena,
Bella L Colombini,
Adriana M Calvo,
Carla R Sipert,
Thiago J Dionísio,
José R P Lauris,
Flávio A C Faria,
Alceu S Trindade Junior,
Carlos F Santos
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ABSTRACT: This study compared the clinical efficacy of 4% articaine (A200) and 0.5% bupivacaine (B200), both with 1:200,000 epinephrine, for lower third molar removal.
Fifty patients underwent removal of symmetrically positioned lower third molars, in 2 separate appointments, under local anesthesia either with A200 or B200, in a double-blind, randomized, and crossover manner. Time to onset, duration of postoperative analgesia, duration of anesthetic action on soft tissues, intraoperative bleeding, and hemodynamic parameters were evaluated.
A statistically significant difference between the time to onset of A200 (1.66 +/- 0.13 minutes) and B200 (2.51 +/- 0.21 minutes) was found (P < .05). There was no statistically significant difference in the duration of analgesia, whether the patient was subjected to osteotomy or not, regardless of the local anesthetic used (3 to 4 hours; P > .05). However, when patients received B200 they experienced a statistically significant longer period of anesthesia on the soft tissues as compared with when they had received A200 (around 5 hours and 4 hours, respectively, P < .05). The surgeon's rating of intraoperative bleeding was considered very close to minimal for both anesthetics. In the surgeries with osteotomy, the comparison between A200 and B200 showed statistically significant differences in the diastolic (64 mm Hg and 68 mm Hg, respectively, P = .001) and mean arterial pressure (86 mm Hg and 89 mm Hg, respectively, P = .031) when data from all the surgical phases were pooled. Additionally, the mouth opening at the suture removal was statistically different for A200 and B200 solutions (91.90% +/- 3.00% and 88.57% +/- 2.38% of the preoperative measure, respectively) when surgeries required bone removal (P < .05).
In comparison with 0.5% bupivacaine, 4% articaine (both with 1:200,000 epinephrine) provided a shorter time to onset and comparable hemostasis and postoperative pain control with a shorter duration of soft tissue anesthesia in lower third molar removal.
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 07/2008; 106(1):19-28. · 1.50 Impact Factor
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ABSTRACT: resUmo Introdução: O objetivo deste estudo é comparar os índices ceos e ceos modificado em levantamento epide-miológico de saúde bucal, em bebês e pré-escolares, segundo a distribuição de cárie dentária no município de Bauru, São Paulo, Brasil. Métodos: Para o cálculo do ceos modificado, foram incluídas lesões de mancha branca. A amostra consistiu de 691 crianças, nas quais foram avaliadas as superfícies dentárias utilizando-se ambos os índices. As crianças foram divididas em 5 grupos de acordo com a idade: G1 (12 – 24 meses), G2 (25 – 36 meses), G3 (37 – 48 meses), G4 (49 – 60 meses) e G5 (61 – 72 meses). Os dados foram ana-lisados pelos testes Mann-Whitney, ANOVA e Tukey (p<0,05). As médias do ceos e do ceos modificado foram 1,56 e 1,79, respectivamente (p<0,001). Resultados: Os resultados mostraram diferença significativa entre os grupos em relação ao ceos e ceos modificado (p<0,05), exceto entre G4 e G5. Conclusão: Portanto, é recomendável a utilização do índice ceos modificado em levantamentos epidemiológicos de cárie dentária incluindo mancha branca. Estes achados confirmam a validez do índice ceos modificado, sugerindo a ne-cessidade de estudos adicionais para testarem a aplicabilidade em populações maiores e diferentes. desCritores: Cárie dentária – Saúde bucal – Saúde infantil – Epidemiologia abstraCt
Revista de Odontologia da Universidade Cidade de São Paulo. 05/2008; 20(2):128-133.
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ABSTRACT: Dental caries is a transmissible infectious disease in which mutans streptococci are generally considered to be the main etiological agents. Although the transmissibility of dental caries is relatively well established in the literature, little is known whether information regarding this issue is correctly provided to the population. The present study aimed at evaluating, by means of a questionnaire, the knowledge and usual attitude of 640 parents and caretakers regarding the transmissibility of caries disease. Most interviewed adults did not know the concept of dental caries being an infectious and transmissible disease, and reported the habit of blowing and tasting food, sharing utensils and kissing the children on their mouth. 372 (58.1%) adults reported that their children had already been seen by a dentist, 264 (41.3%) answered that their children had never gone to a dentist, and 4 (0.6%) did not know. When the adults were asked whether their children had already had dental caries, 107 (16.7%) answered yes, 489 (76.4%) answered no, and 44 (6.9%) did not know. Taken together, these data reinforce the need to provide the population with some important information regarding the transmission of dental caries in order to facilitate a more comprehensive approach towards the prevention of the disease.
Journal of applied oral science: revista FOB 05/2008; 16(2):150-4. · 0.39 Impact Factor
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ABSTRACT: Traumatic dental injuries are relatively frequent accidents that typically involve teeth in the maxillary anterior segment. The emergency treatment and the clinical decisions must be efficiently made at the time of injury, and there is a need for long-term follow-up because of the high incidence of complications. The aim of this article was to present the emergency and rehabilitation treatments of a multiple dentoalveolar trauma in the permanent dentition involving different extensions of enamel-dentin crown fracture, pulp exposure, and the avulsion of a canine. The treatment outcomes are reported up to the 4-year follow-up, and the clinical approaches and their rationale are discussed.
Journal of Endodontics 04/2008; 34(3):336-9. · 2.88 Impact Factor
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ABSTRACT: ental caries is a transmissible infectious disease in which mutans streptococci are generally considered to be the main etiological agents. Although the transmissibility of dental caries is relatively well established in the literature, little is known whether information regarding this issue is correctly provided to the population. The present study aimed at evaluating, by means of a questionnaire, the knowledge and usual attitude of 640 parents and caretakers regarding the transmissibility of caries disease. Most interviewed adults did not know the concept of dental caries being an infectious and transmissible disease, and reported the habit of blowing and tasting food, sharing utensils and kissing the children on their mouth. 372 (58.1%) adults reported that their children had already been seen by a dentist, 264 (41.3%) answered that their children had never gone to a dentist, and 4 (0.6%) did not know. When the adults were asked whether their children had already had dental caries, 107 (16.7%) answered yes, 489 (76.4%) answered no, and 44 (6.9%) did not know. Taken together, these data reinforce the need to provide the population with some important information regarding the transmission of dental caries in order to facilitate a more comprehensive approach towards the prevention of the disease.
Journal of Applied Oral Science 03/2008; 16(2):150-154. · 0.57 Impact Factor
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ABSTRACT: F urcal perforations may occur during access opening of the pulp chamber or cavity preparation. The perfora-tion can cause an inflammatory reaction and severely compromise the tooth's prognosis. Such a perforation results in loss of the root's integrity and further destruction of the adjacent periodontal tissues. 1 Recently, mineral trioxide aggregate (MTA) has been used for several dental purposes. 2-11 MTA is a mineral pow- der that consists of hydrophilic particles, whose principal components are tricalcium silicate, tricalcium aluminate, tricalcium oxide, and other mineral oxides. It has a pH of 12.5, and sets in the presence of moisture in approximately 4 hours. 2,3,9 Studies have shown that MTA apparently is equal or superior to other materials concerning dye and bacterial contamination. 2-6 Its benefits include good sealing ability, 2,3 biocompatibility, 2-6 low cytotoxicity, 2,3,7 and promotion of odontoblasts to form a hard barrier. 2,3 MTA is suggested for root-end filling, 2,3 pulp capping, 7 repair of furcal perforations, 10-15 apical filling of teeth with open apices, and apexification therapy. 3,8 MTA has been pro-posed as a potential medicament for pulpotomy, and tests in dogs' teeth produced favorable pulp responses. 8,13 When compared with other materials used for these purposes, similar or better biocompatibility has been reported. 7,8,16,17 ABSTRACT
Journal of dentistry for children 03/2008; 75(2):188-191.
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Journal of dentistry for children 03/2008; 75(2):188-191.
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ABSTRACT: Epidermolysis bullosa (EB) consists of a group of genetic hereditary disorders in which patients frequently present fragile skin and mucosa that form blisters following minor trauma. More than 20 subtypes of EB have been recognized in the literature. Specific genetic mutations are well characterized for most the different EB subtypes and variants. The most common oral manifestations of EB are painful blisters affecting all the oral surfaces. Dental treatment for patients with EB consists of palliative therapy for its oral manifestations along with typical restorative and periodontal procedures. The aim of this article is to describe two dental clinical treatments of recessive dystrophic EB cases and their specific clinical manifestations. The psychological intervention required during the dental treatment of these patients is also presented.
Journal of applied oral science: revista FOB 03/2008; 16(1):81-5. · 0.39 Impact Factor
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Carlos F Santos,
Karin C S Modena,
Fernando P M Giglio, Vivien T Sakai,
Adriana M Calvo,
Bella L Colombini,
Carla R Sipert,
Thiago J Dionísio,
Flávio A C Faria,
Alceu S Trindade,
José R P Lauris
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ABSTRACT: This study compared the use of 4% articaine in association with 1:100,000 (10 mug/mL; A100) or 1:200,000 (5 mug/mL; A200) epinephrine in lower third molar removal.
Fifty healthy volunteers underwent removal of symmetrically positioned lower third molars, in 2 separate appointments, under local anesthesia with either A100 or A200, in a double-blind, randomized, and crossed manner. Latency, duration of postoperative analgesia, duration of anesthetic action on soft tissues, intraoperative bleeding, and hemodynamic parameters were evaluated.
A100 and A200 presented very similar latency (1.64 +/- 0.08 and 1.58 +/- 0.08 minutes, respectively; P > .05). Identical volumes of both anesthetic solutions were used: 2.7 mL = 108 mg of articaine plus 27 mug (A100) or 13.5 mug (A200) of epinephrine. The 2 solutions provided similar duration of postoperative analgesia regardless of bone removal (around 200 minutes; P > .05). The 2 solutions also had a similar duration of anesthetic action on soft tissues (around 250 minutes; P > .05). The surgeon's rating of intraoperative bleeding was considered very close to minimal. Transient changes in hemodynamic parameters were observed, but these were neither clinically significant nor attributable to the type of anesthetic used (P > .05).
An epinephrine concentration of 1:100,000 or 1:200,000 in 4% articaine solution does not affect the clinical efficacy of this local anesthetic. It is possible to successfully use the 4% articaine formulation with a lower concentration of epinephrine (1:200,000 or 5 mug/mL) for lower third molar extraction with or without bone removal.
Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons 12/2007; 65(12):2445-52. · 1.58 Impact Factor
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ABSTRACT: This paper describes the case of a 7-day-old girl who was referred to a paediatric dentistry clinic because of the presence of a pedunculated mass protruding from the front of her mouth.
The mass was attached to the maxillary alveolus to the right of the midline, and was clinically diagnosed as a congenital epulis. The baby had no airway obstruction and was able to feed well. A conservative treatment was proposed, with monthly follow-up appointments to monitor the lesion.
After 8 months, the lesion had completely regressed, meaning that the girl did not have to be exposed to unnecessary surgical procedures in her first few days of life. The eruption of the upper anterior teeth was not affected.
International Journal of Paediatric Dentistry 08/2007; 17(4):309-12. · 1.01 Impact Factor
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ABSTRACT: RESUMO A utilização de coroas de aço corresponde ao procedimento protético mais efi ciente para a realização de grandes reconstruções coronárias em dentes decíduos e permanentes jovens. Esta revisão de literatura tem como objetivos descrever essa técnica protética restauradora e fornecer subsídios ao odontopediatra e ao clínico geral para corretas indicações clínicas do material, baseados em resultados clínicos e laboratoriais publicados nos últimos anos. Palavras-chave: coroa de aço inoxidável, cárie dentária e crianças ABSTRACT Stainless Steel Crowns: Technique and Cost Effectiveness. Use of stainless steel crowns corresponds to the most effi cient prosthetic technique to accomplish large coronal reconstructions in decidu-ous and young permanent teeth. The aims of the current literature review are to describe this restorative technique and to offer sub-sidies for correct clinic indications of the material to pediatric and
Revista Salusvita. 07/2007; 26(3):135-152.
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Journal of dentistry for children 05/2007; 74:200-202.
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ABSTRACT: This study aimed at evaluating the prevalence of putative periodontal pathogens (Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella nigrescens, Treponema denticola) in saliva of children with mixed dentition at two different time points, correlating these findings with a clinical parameter of gingival health.
Polymerase chain reaction (PCR) detection was used to determine the prevalence of these bacteria in saliva of 64 children in 2003 and 60 children in 2004. Gingival health was assessed by gingival index.
Sixty-two (96.9%) and 50 (83.3%) children presented low gingival inflammation, whereas 2 (3.1%) and 10 (16.7%) had moderate scores in 2003 and 2004, respectively. Majority of the children (81.3% in 2003 and 73.3% in 2004) had detectable levels of at least one of the bacteria. The prevalence found was of 4.7% and 1.7% for A. actinomycetemcomitans, 6.3% and 8.3% for P. gingivalis, 23.4% and 48.3% for P. nigrescens (P < 0.05), and 71.9% and 50% for T. denticola (P < 0.05) in 2003 and 2004, respectively. No significant relationship between gingival index and presence of these bacteria and combination of different species was found.
A high percentage of children harboured at least one of the putative periodontal pathogens in saliva, but presented periodontally healthy conditions.
International Journal of Paediatric Dentistry 05/2007; 17(3):192-9. · 1.01 Impact Factor
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ABSTRACT: This work aimed at performing a retrospective and comparative investigation of pharmacological therapeutic approach for pain and inflammation control for cleft lip and/or palate repair.
Medical charts from 2000 patients who underwent surgical procedures at the Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo (HRAC-USP), Brazil, were assessed to obtain information regarding type of cleft, surgical procedure, and analgesic and anti-inflammatory drugs prescribed. The first 1000 consecutive surgeries performed in 1992 and 2002 were assessed.
Different analgesic and anti-inflammatory agents-nonsteroidal anti-inflammatory drugs (NSAIDS), steroids, and opioids-were given to patients perioperatively and postoperatively. NSAIDS were given to almost all patients (97.03% in 1992 and 99.88% in 2002, P > .05). Steroid administration increased in 2002 (8.66% versus 17.71%, P < .05). Opioids were administered only in 2002 (50.31%, P < .05).
NSAIDS, steroids, and opioids were used for pain and inflammation control in cleft lip and palate repair at HRAC-USP. A change in the pattern of analgesic and anti-inflammatory drug use was observed when comparing 1992 and 2002. More potent compounds, such as opioids, were used in 2002 in a significant percentage of all the surgical procedures.
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 11/2006; 102(4):e16-20. · 1.50 Impact Factor
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ABSTRACT: Comparison of the clinical efficacy of 4% articaine in relation to 2% mepivacaine, both with 1:100,000 epinephrine, in the prevention of postoperative pain after lower third molar removal.
Twenty patients underwent removal of bilateral lower third molars under local anesthesia (articaine or mepivacaine) in 2 separate appointments, in a double-blind, randomized, and crossed manner. Objective and subjective parameters were recorded for paired comparison of postoperative courses.
Duration of analgesia provided by articaine and mepivacaine was 198.00 +/- 25.86, and 125.40 +/- 13.96 min, respectively (P = .02), whereas the duration of anesthesia was 273.80 +/- 15.94 and 216.85 +/- 20.15 min, respectively (P = .06). Both solutions exerted no important effects upon arterial pressure, heart rate, or oxygen saturation (P > .05).
Articaine provides a longer period of analgesic effect and a tendency for a longer period of anesthesia as compared to mepivacaine. The presence of a vasoconstrictor agent in local anesthetic solutions does not seem to influence hemodynamic parameters during lower third molar removal in healthy subjects.
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 08/2006; 102(2):169-74. · 1.50 Impact Factor
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International Journal of Paediatric Dentistry 07/2006; 17:309-312. · 1.01 Impact Factor
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