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ABSTRACT: To reconstruct segmental mandibulectomy using calcium phosphate ceramics and collagen membrane with a delayed bone marrow grafting in experimental animals.
Defects of segmental mandibulectomy were filled with calcium phosphate granules and wrapped with a collagen membrane in 4 dogs and left empty as a control in 2 dogs. Two months later, a bone marrow graft was injected into the center of the implants. Animals were humanely killed after a 16-week delay.
Six adult beagles were included in this study.
Segmental mandibulectomy.
Bone ingrowth and material resorption in the reconstructed segment.
Successful osseous colonization bridged the whole length of the defects. The good new bone formation at the center and the periosteum-like formation at the periphery suggest the osteoinductive role of the bone marrow graft and the healing scaffold role of the membrane.
This model succeeded in regenerating a large segmental defect in the mandible. An investigation with a postimplantation radiation delivery schedule is required with the use of this model, which should be considered as a preclinical study for a bone tissue engineering approach in patients with cancer-related bone defects.
Archives of otolaryngology--head & neck surgery 10/2010; 136(10):971-8. · 1.92 Impact Factor
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ABSTRACT: Oral carcinomas are frequently treated with a nonsegmental mandibulectomy plus radiotherapy. Improving the quality of life of these patients depends on the possibilities for dental rehabilitation. The aim of this study was to increase the alveolar ridge height. Twelve white New Zealand rabbits underwent surgery to produce a 11 x 9 x 7 mm(3) horizontal bicortical full-thickness defect at the alveolar ridge of the left mandible. Six were implanted with a composite associating resorbable collagen membrane filled with micro-macroporous biphasic calcium phosphate granules. After a daily radiation delivery schedule for 4 weeks, a total autologous bone marrow graft was injected percutaneously into the center of the implant. All animals were sacrificed at 16 weeks. Successful osseous colonization was observed in all implants. Significant ridge augmentation was observed (p = 0.0349) in the implanted group compared with the control group. This study contributed to producing an experimental model for oncological mandible defects in rabbits.
Journal of Biomedical Materials Research Part A 12/2009; 93(4):1519-26. · 2.63 Impact Factor
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ABSTRACT: The purpose of this study was to compare bone reconstruction using either mesenchymal stem cells (MSCs) or total bone marrow (TBM) in association with biphasic calcium phosphate (BCP) granules after irradiation in a rat model. Three weeks after an external irradiation of the hind limbs of rats, four bone defects were created per animal. The defects were filled with either BCP alone, or with a mixture of BCP and TBM, or with a mixture of BCP and MSCs (adipose-derived or bone marrow-derived MSCs). Three weeks after implantations, new-bone formation was assessed. Histological examination showed osteoconductive and osteointegrative properties of BCP in irradiated tissue. The BCP-TBM mixture significantly improved bone ingrowth (p<0.05). The BCP-MSCs mixtures did not provide new-bone formation over and above that induced by BCP alone. This gives grounds for suspecting that there is a link between this result and the cellular and vascular weakness observed in irradiated bone. The BCP-TBM mixture may have induced an increased vascularization of irradiated bone. This could be due to the presence of all components in TBM that were lacking in the BCP-MSCs mixtures. BCP associated with TBM appears to be the most efficient material for bone substitution in irradiated areas.
Biomaterials 12/2008; 30(5):763-9. · 7.40 Impact Factor
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ABSTRACT: Some biomaterials are able to induce ectopic bone formation in muscles of large animals. The osteoinductive potential of macro- micro-porous biphasic calcium phosphate (MBCP) ceramic granules with fibrin glue was evaluated by intramuscular implantation for 6 months in six adult female sheep. The MBCP granules were 1-2 mm in size and were composed of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) in a 60/40 ratio. The fibrin glue was composed of fibrinogen, thrombin and other biological factors. After 6 months of implantation in the dorsal muscles of sheep, the explants were rigid. Histology, back-scattered electron microscopy and micro-computed tomography of the implants indicated that approximately 12% of mineralized bone had formed in between the MBCP granules. The ectopic bone appeared well-mineralized with mature osteocytes and Haversian structures. In addition, the number and thickness of bone trabeculae formed in between the MBCP particles were similar to those measured in trabecular bone in sheep. The overall results therefore confirmed the formation of well-mineralized ectopic bone tissue after intramuscular implantation of MBCP/fibrin glue composites. These bone substitutes exhibiting osteoinductive properties could be used for the reconstruction of large bone defects.
Journal of Materials Science Materials in Medicine 03/2008; 19(2):667-75. · 2.32 Impact Factor
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ABSTRACT: Calcium phosphate (Ca-P) ceramics are currently used in various types of orthopaedic and maxillofacial applications because of their osteoconductive properties. Fibrin glue is also used in surgery due to its haemostatic, chemotactic and mitogenic properties and also as scaffolds for cell culture and transplantation. In order to adapt to surgical sites, bioceramics are shaped in blocks or granules and preferably in porous forms. Combining these bioceramics with fibrin glue provides a mouldable and self-hardening composite biomaterial. The aim of this work is to study the osteogenic properties of this composite material using two different animal models. The formation of newly formed bone (osteoinduction) and bone healing capacity (osteconduction) have been study in the paravertebral muscles of sheep and in critical sized defects in the femoral condyle of rabbits, respectively. The different implantations sites were filled with composite material associating Ca-P granules and fibrin glue. Ca-P granules of 1-2 mm were composed with 60% of hydroxyapatite and 40% of beta tricalcium phosphate in weight. The fibrin glue was composed of fibrinogen, thrombin and other biological factors. After both intramuscular or intraosseous implantations for 24 weeks and 3, 6, 12 and 24 weeks, samples were analyzed using histology and histomorphometry and mechanical test. In all cases, the newly formed bone was observed in close contact and around the ceramic granules. Depending on method of quantification, 6.7% (with BSEM) or 17% (with micro CT) of bone had formed in the sheep muscles and around 40% in the critical sized bone rabbit defect after 24 weeks. The Ca-P/fibrin material could be used for filling bone cavities in various clinical indications.
Journal of Materials Science Materials in Medicine 03/2007; 18(2):225-35. · 2.32 Impact Factor
