Jae-Hong Kim

Korea University, Sŏul, Seoul, South Korea

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Publications (98)284.32 Total impact

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    A-Jin Lee, Kyung-Jin Cho, Jae-Hong Kim
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    ABSTRACT: Endotoxic responses to bacterial lipopolysaccharide (LPS) are triggered by Toll-like receptor 4 (TLR4) and involve the production of inflammatory mediators, including interleukin-6 (IL-6), by macrophages. The detailed mechanism of IL-6 production by macrophages in response to LPS has remained unclear, however. We now show that LPS induces IL-6 synthesis in mouse peritoneal macrophages via the leukotriene B4 receptor BLT2. Our results suggest that TLR4-MyD88 signaling functions upstream of BLT2 and that the generation of reactive oxygen species (ROS) by NADPH oxidase 1 (Nox1) and consequent activation of the transcription factor nuclear factor (NF)-κB function downstream of BLT2 in this response. These results suggest that a TLR4-MyD88-BLT2-Nox1-ROS-NF-κB pathway contributes to the synthesis of IL-6 in LPS-stimulated mouse macrophages.
    04/2015; 47(4):e156. DOI:10.1038/emm.2015.8
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    ABSTRACT: Objective: This study addresses common errors that may occur during digital impression procedures using the CEREC AC and in-office CAD/CAM systems. Error types and frequencies resulting from digital impressions of the oral cavity were investigated and their origins identified to facilitate the acquisition of more accurate digital impressions. Method and Materials: A total of 1,251 digital impression cases, including 163 onlays and 1,088 inlays, were constructed as restorations using the CEREC AC system. Two evaluators determined five categories of digital errors as a basis for evaluation over two sessions. The five categories were as follows: inappropriate scanner positioning (ISP), improper handling of the scanner (IHS), irregular powder arrangement (IPA), improper cavity preparation (ICP), and insufficient scanned data (ISD). Results: The most frequently encountered errors were non-linear powder application (IPA), inappropriate oral cavity scanner placement (ISP), and insufficient data (ISD). ISP showed that inlays had a slightly higher frequency of errors, but this observation was not statistically significant (P > .05). Conclusion: Most errors are caused by incorrect operation of intraoral scanners or uneven application of powder in acquiring digital impressions. To provide optimal digital impression results, careful chairside technique procedures and operation of intraoral scanners are required.
    Quintessence international (Berlin, Germany: 1985) 02/2015; DOI:10.3290/j.qi.a33685 · 0.73 Impact Factor
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    ABSTRACT: The clinical applicability of CAD/CAM-fabricated zirconia copings is tested using working models made from four different highstrength Type IV gypsum materials. Each of the four materials was used to fabricate 15 zirconia copings. Precision of fit was measured with a digital electron microscope using the silicone replica technique. The mean and standard deviation of each reference point were analyzed using the one-way analysis of the variance (ANOVA) and Tukey’s honest significant difference (HSD) tests (α=0.05). The overall marginal and internal fits of the zirconia copings were as follows: GS (GS: Grey Stone) group: 91.43 μm, LS (LS: Light green Stone) Group: 87.89 μm, RS (RS: Red Stone) Group: 88.75 μm, BS (BS: Beige Stone) Group: 82.78 μm. There were no significant differences between the mean adaptations of the gypsum varieties (p>0.05). This confirmed that the type of gypsum material used does not determine the precision of fit of a prosthesis.
    Dental Materials Journal 02/2015; 34(1). DOI:10.4012/dmj.2014-141 · 0.94 Impact Factor
  • Geun-Soo Park, Jae-Hong Kim
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    ABSTRACT: Inflammation and local inflammatory mediators are inextricably linked to tumor progression through complex pathways in the tumor microenvironment. Lipopolysaccharide (LPS) exposure to tumor cells has been suggested to promote tumor invasiveness and metastasis. However, the detailed signaling mechanism involved has not been elucidated. In this study, we showed that LPS upregulated the expression of leukotriene B4 receptor-2 (BLT2) and the synthesis of BLT2 ligands in MDA-MB-231 and MDA-MB-435 breast cancer cells, thereby promoting invasiveness. BLT2 depletion with siRNA clearly attenuated LPS-induced invasiveness. In addition, we demonstrated that myeloid differentiation primary response gene 88 (MyD88) lies upstream of BLT2 in LPS-potentiated invasiveness and that this 'MyD88-BLT2' cascade mediates activation of NF-κB and the synthesis of IL-6 and IL-8, which are critical for the invasiveness and aggression of breast cancer cells. LPS-driven metastasis of MDA-MB-231 cells was also markedly suppressed by the inhibition of BLT2. Together, our results demonstrate, for the first time, that LPS potentiates the invasiveness and metastasis of breast cancer cells via a 'MyD88-BLT2'-linked signaling cascade.
    Oncotarget 01/2015; · 6.63 Impact Factor
  • Sa-Hak Kim, Jae-Hong Kim
    12/2014; 14(4):455-460. DOI:10.17135/jdhs.2014.14.4.455
  • Ki-Baek Kim, Seok-Hwan Kim, Jae-Hong Kim
    09/2014; 14(3):405-410. DOI:10.17135/jdhs.2014.14.3.405
  • Jae-Hong Kim, Won-Soo Kim, Ki-Baek Kim
    09/2014; 14(3):349-355. DOI:10.17135/jdhs.2014.14.3.349
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    ABSTRACT: One of the most important factors in determining the clinical outcomes of fixed dental prostheses is the gap between the fixed dental prosthesis and the abutment. However, reports that investigated these gaps in the context of fixed dental prostheses fabricated with new technologies are few.
    Journal of Prosthetic Dentistry 09/2014; 112(6). DOI:10.1016/j.prosdent.2014.07.002 · 1.42 Impact Factor
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    ABSTRACT: The purpose of this study was to investigate, by measuring the gap, the possible clinical use of three-unit fixed dental prostheses (FDPs) manufactured using stereolithography. A total of 20 epoxy models were built with a same case (abutment teeth 14, 16). The 40 specimens were produced using the stereolithography (SLA) and wax-up (LW). The 960 gaps of the 40 specimens produced were measured by a silicone replica. The Wilcoxon rank sum test was then used to compare and analyze the data obtained from the two groups (α=0.05). The total gap, as measured from the SLA and LW groups, was 98.6 and 66.6 µm, respectively. The results indicate that the gap in the SLA group is statistically significantly greater than that in the LW group (p<0.05). Further assessment and improvement of the SLA method for the fabrication of FDPs is evidently still required.
    Dental Materials Journal 08/2014; 33(4):504-9. DOI:10.4012/dmj.2014-017 · 0.94 Impact Factor
  • Hyunju Kim, Jung-A Choi, Jae-Hong Kim
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    ABSTRACT: Inflammation and inflammatory mediators are inextricably linked with epithelial-mesenchymal transition (EMT) through complex pathways in the tumor microenvironment. However, the mechanism by which inflammatory mediators, such as the lipid inflammatory mediators, eicosanoids, contribute to EMT is largely unknown. In the present study, we observed that BLT2, a leukotriene B4 (LTB4) receptor-2, is markedly up-regulated by oncogenic Ras and promotes EMT in response to transforming growth factor-β (TGF-β) in mammary epithelial cells. Blockade of BLT2 by the BLT2 inhibitor LY255283 or by siRNA reduced EMT induced by Ras in the presence of TGF-β. In addition, stimulation of BLT2 by the addition of a BLT2 ligand, such as LTB4, restored EMT in the presence of TGF-β in human immortalized mammary epithelial MCF-10A cells. We further searched BLT2 downstream components and identified reactive oxygen species (ROS) and nuclear factor (NF)-κB as critical components that contribute to EMT. Taken together, these results demonstrate for the first time that a BLT2-linked inflammatory pathway contributes to EMT. This provides valuable insight into the mechanism of EMT in mammary epithelial cells. In addition, considering the implications of EMT with the stemness of cancer cells, our finding may contribute to a better understanding of tumor progression.
    Journal of Biological Chemistry 07/2014; 289(32). DOI:10.1074/jbc.M114.556126 · 4.60 Impact Factor
  • Jae-Hong Kim, Woong-Chul Kim, Ji-Hwan Kim
    06/2014; 36(2):75-81. DOI:10.14347/kadt.2014.36.2.75
  • Jae-Hong Kim, Jae-Kwan Jung, Ki-Baek Kim
    05/2014; 15(5):3007-3013. DOI:10.5762/KAIS.2014.15.5.3007
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    ABSTRACT: PURPOSEThe objective of this study was to evaluate the clinical acceptability of all-ceramic crowns fabricated by the digital veneering method vis-à-vis the traditional method.MATERIALS AND METHODSZirconia specimens manufactures by two different manufacturing method, conventional vs digital veneering, with three different thickness (0.3 mm, 0.5 mm, 0.7 mm) were prepared for analysis. Color measurement was performed using a spectrophotometer for the prepared specimens. The differences in shade in relation to the build-up method were calculated by quantifying ΔE* (mean color difference), with the use of color difference equations representing the distance from the measured values L*, a*, and b*, to the three-dimensional space of two colors. Two-way analysis of variance (ANOVA) combined with a Tukey multiple-range test was used to analyze the data (α=0.05).RESULTSIn comparing means and standard deviations of L*, a*, and b* color values there was no significant difference by the manufacturing method and zirconia core thickness according to a two-way ANOVA. The color differences between two manufacturing methods were in a clinically acceptable range less than or equal to 3.7 in all the specimens.CONCLUSIONBased on the results of this study, a carefully consideration is necessary while selecting upper porcelain materials, even if it is performed on a small scale. However, because the color reproducibility of the digital veneering system was within the clinically acceptable range when comparing with conventional layering system, it was possible to estimate the possibility of successful aesthetic prostheses in the latest technology.
    The journal of advanced prosthodontics 04/2014; 6(2):71-8. DOI:10.4047/jap.2014.6.2.71 · 0.63 Impact Factor
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    Seog-Soon Choi, Jae-Hong Kim, Ji-Hwan Kim
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    ABSTRACT: Purpose: The purpose of this study was to analyze and compare the relative accuracy of digitized stone models of lower full arch, using two different scanning system. Methods: Replica stone models(N=20) were produced from lower arch acrylic model. Twenty digital models were made with the white light and blue LED(, Korea) scanner. Two-dimensional distance between the landmarks were measured on the Delcam (Delcam plc, UK). Independent samples t-test was applied for comparison of the groups. All statistical analyses were performed using the SPSS software package(Statistical Package for Social Sciences for Windows, version 12.0). Results: The absolute disagreement between measurements made directly on the two different scanner-based dental digital models was 0.02~0.04mm, and was not statistically significant(P>0.05). Conclusion: The precision of the blue LED optical scanner was comparable with the digitization device, and relative accuracy was similar. However, there still is room for improvement and further standardization of dental CAD technologies.
    03/2014; 36(1). DOI:10.14347/kadt.2014.36.1.17
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    ABSTRACT: This study aimed to evaluate the accuracy and precision of polyurethane (PUT) dental arch models fabricated using a three-dimensional (3D) subtractive rapid prototyping (RP) method with an intraoral scanning technique by comparing linear measurements obtained from PUT models and conventional plaster models. Ten plaster models were duplicated using a selected standard master model and conventional impression, and 10 PUT models were duplicated using the 3D subtractive RP technique with an oral scanner. Six linear measurements were evaluated in terms of x, y, and z-axes using a non-contact white light scanner. Accuracy was assessed using mean differences between two measurements, and precision was examined using four quantitative methods and the Bland-Altman graphical method. Repeatability was evaluated in terms of intra-examiner variability, and reproducibility was assessed in terms of inter-examiner and inter-method variability. The mean difference between plaster models and PUT models ranged from 0.07 mm to 0.33 mm. Relative measurement errors ranged from 2.2% to 7.6% and intraclass correlation coefficients ranged from 0.93 to 0.96, when comparing plaster models and PUT models. The Bland-Altman plot showed good agreement. The accuracy and precision of PUT dental models for evaluating the performance of oral scanner and subtractive RP technology was acceptable. Because of the recent improvements in block material and computerized numeric control milling machines, the subtractive RP method may be a good choice for dental arch models.
    Korean Journal of Orthodontics 03/2014; 44(2):69-76. DOI:10.4041/kjod.2014.44.2.69 · 0.37 Impact Factor
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    ABSTRACT: Purpose: The aim of this study was to compare with color reproducibility of the coating method and dipping method using a colour liquid. Methods: Two groups of square-shaped specimens ( in diameter, 1.5mm thickness) were prepared for analysis(n=15/group). The CM(Coating Method) group was fabricated as full zirconia specimens with the coating coloring method and Group DM(Dipping Method) was prepared as specimens of 0.7 mm-thick zirconia plate using the dipping coloring method and veneered with 0.8mm-thick porcelain. Color parameters were measured with ShadeEye spectrophotometer and color differences were calculated using the equation . Non-parametric statistical methods, the Mann-Whitney test, and the Kruskal-Wallis test were used to analyze the data. Type one error rate was set at 0.05. Results: Color difference values larger than 7 were observed between specimens with the coating method and the dipping method, which were detectable difference in a clinical setting. Conclusion: Cautious application of the coating coloring method in a clinical setting and further researches to standardize the coating method are needed.
    09/2013; 35(3). DOI:10.14347/kadt.2013.35.3.185
  • Seok-Hwan Kim, Jae-Hong Kim
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    ABSTRACT: Purpose: The purpose of this study was to investigate the image and the awareness of dental laboratory technicians in health allied college students. Methods: The subject of this research are 431 health-related major students who are selected by convenience sampling from Seoul, Gyeonggi-do, Chungcheong-do, Jeolla-do and Gyeongsang-do. After a survey was conducted, the answer sheets from 401 respondents were analyzed except for 30 incomplete ones. The collected data is statistically analyzed by SPSS(Statistical Packages for Social Science 12.0. SPSS Inc., Chicago, IL, USA). Results: The findings of the study were as follows: As a result of general awareness about dental laboratory technicians, the health allied college students knew 83.0% about dental laboratory technician as a job. Respondents showed high recognition about dental laboratory technician as a job(), which showed that the health allied college students took a favorable view of dental laboratory in general. There were significant difference in dental laboratory technician image with age, gender(P
    09/2013; 35(3). DOI:10.14347/kadt.2013.35.3.221
  • Jin-Wook Lee, Jae-Hong Kim
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    ABSTRACT: The majority of prostate cancer-related deaths are associated with advanced and metastatic malignancies. Although anoikis resistance has been recognized as one of the hallmarks of metastatic prostate malignancies, the molecular events that cause anoikis resistance are poorly understood. In this study, we found that the detachment of PC-3 prostate cancer cells caused a time-dependent increase in the expression level of the leukotriene B4 receptor-2 (BLT2) and that BLT2 played a critical role in establishing anoikis resistance in these cells. Blocking BLT2 with the pharmacological inhibitor LY255283 or with RNAi knockdown clearly abolished anoikis resistance and resulted in severe apoptotic death. Additionally, we demonstrated that the activation of NADPH oxidase (NOX) and subsequent generation of reactive oxygen species (ROS) were downstream of BLT2 signaling and led to the activation of NF-κB, thus establishing anoikis resistance during cell detachment. Furthermore, we observed that the ectopic expression of BLT2 in normal prostate PWR-1E cells rendered the cells resistant to anoikis and apparently diminished apoptotic cell death following detachment. Taken together, our results suggest that BLT2-NOX-ROS-NF-κB cascade induction during detachment confers a novel mechanism of anoikis resistance in prostate cancer cells and potentially contributes to prostate cancer progression.
    Journal of Biological Chemistry 08/2013; DOI:10.1074/jbc.M113.481283 · 4.60 Impact Factor
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    ABSTRACT: BLT2, a low-affinity receptor for leukotriene B4 (LTB4), is a member of the G protein-coupled receptor (GPCR) family and is involved in many signal transduction pathways associated with various cellular phenotypes, including chemotactic motility. However, the regulatory mechanism for BLT2 has not yet been demonstrated. To understand the regulatory mechanism of BLT2, we screened and identified the proteins that bind to BLT2. Using a yeast two-hybrid assay with the BLT2 C-terminal domain as bait, we found that RanBPM, a previously proposed scaffold protein, interacts with BLT2. We demonstrated the specific interaction between BLT2 and RanBPM by GST pull-down assay and co-immunoprecipitation assay. To elucidate the biological function of the RanBPM-BLT2 interaction, we evaluated the effects of RanBPM overexpression or knockdown. We found that BLT2-mediated motility were severely attenuated by RanBPM overexpression and knock-down of endogenous RanBPM by shRNA strongly promoted BLT2-mediated motility, suggesting a negative regulatory function of RanBPM toward BLT2. Furthermore, we observed that the addition of BLT2 ligands caused the dissociation of BLT2 and RanBPM, thus releasing the negative regulatory effect of RanBPM. Finally, we propose that Akt-induced BLT2 phosphorylation at residue Thr355, which occurs after the addition of BLT2 ligands, is a potential mechanism by which BLT2 dissociates from RanBPM, resulting in stimulation of BLT2 signaling. Taken together, our results suggest that RanBPM acts as a negative regulator of BLT2 signaling to attenuate BLT2-mediated cell motility.
    Journal of Biological Chemistry 08/2013; 288(37). DOI:10.1074/jbc.M113.470260 · 4.60 Impact Factor
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    Ki-Baek Kim, Joo-Hwan Kim, Jae-Hong Kim
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    ABSTRACT: The purpose of this study is to research how to effectively export the dental prosthetic restoration and the service to the overseas. The study showed the present status of dental prosthetic restoration service in South Korea and some suggested ways to improve the export of the dental prosthetic restoration service to the overseas. The size of the medical service market has been gradually increased because of the globalization and the development of the information-oriented tendency. The medical industry is being perceived as a highly valuable service industry that emphasizes the importance of quality. Through the efforts of the nations attempting to develop the market and create new employment, the medical field is becoming a prospective field of trade. In order to develop the medical industry, the strategies; firstly, medical institutions need to explore overseas markets and strengthen globalization; secondly, medical service should be upgraded through specialization; and finally, specialized management system has to be established and medical management system has to be innovated.
    07/2013; 13(7). DOI:10.5392/JKCA.2013.13.07.333

Publication Stats

2k Citations
284.32 Total Impact Points

Institutions

  • 2002–2015
    • Korea University
      • • Dental Laboratory of Science and Engineering
      • • Department of Mechanical Engineering
      Sŏul, Seoul, South Korea
  • 2008
    • Chonbuk National University Hospital
      Sŏul, Seoul, South Korea
  • 2005
    • University of Seoul
      Sŏul, Seoul, South Korea
    • Gwangju Institute of Science and Technology
      • Department of Life Sciences
      Kwangju, Gwangju, South Korea
  • 2004
    • Korea Institute of Radiological & Medical Sciences
      Sŏul, Seoul, South Korea
  • 2000
    • Korea Basic Science Institute KBSI
      Sŏul, Seoul, South Korea