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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Abdul Qadir Shah,
Jameel Ahmed Baig,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain
[show abstract]
[hide abstract]
ABSTRACT: This study was designed to compare the levels of chromium (Cr) and manganese (Mn) in scalp hair, blood, and urine of night
blindness in children age ranged (3–7) and (8–12) years of both genders, comparing them to sex- and age-matched controls.
A microwave-assisted wet acid digestion procedure, was developed as a sample pretreatment, for the determination of Cr and
Mn in biological samples of night blindness children. The proposed method was validated by using conventional wet digestion
and certified reference samples of hair, blood and urine. The digests of all biological samples were analyzed for Cr and Mn
by electrothermal atomic absorption spectrometry. The results indicated significantly higher levels of Cr, whilst low level
of Mn in the biological samples (blood and scalp hair) of male and female night blindness children, compared with control
subjects of both genders. These data present guidance to clinicians and other professional investigating deficiency of Mn
and excessive level of Cr in biological samples (scalp hair and blood) of night blindness children.
KeywordsNight blindness–Chromium–Manganese–Biological samples–Wet acid digestion methods–Children–Age groups (3–12)–Atomic absorption spectrophotometer
Biological Trace Element Research 04/2012; 143(1):103-115. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain,
Sirajuddin
[show abstract]
[hide abstract]
ABSTRACT: The causes of night blindness in children are multifactorial, and particular consideration has been given to childhood nutritional
deficiency, which is the most common problem found in underdeveloped countries. Such deficiency can result in physiological
and pathological processes that in turn influence hair composition. This study was designed to compare the levels of zinc
(Zn), copper (Cu), and iron (Fe) in scalp hair, blood, and urine of both genders of children with night blindness with age
range of 3–7 and 8–12 years, comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion procedure
was developed as a sample pretreatment, for the determination of zinc, copper, and iron in biological samples of children
with night blindness. The proposed method was validated by using conventional wet digestion and certified reference samples
of hair, blood, and urine. The digests of all biological samples were analyzed for Cu, Fe, and Zn by flame atomic absorption
spectrometry using an air/acetylene flame. The results indicated significantly lower levels of Fe, Cu, and Zn in the biological
samples (blood and scalp hair) of male and female children with night blindness, compared with control subjects of both genders.
These data present guidance to clinicians and other professionals investigating the deficiency of essential trace metals in
biological samples (scalp hair and blood) of children with night blindness.
KeywordsNight blindness–Zinc–Copper–Iron–Biological samples–Wet acid digestion methods–Children–Age groups (3–12)–Atomic absorption spectrophotometer
Biological Trace Element Research 04/2012; 142(3):323-334. · 1.92 Impact Factor
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Tasneem Gul Kazi, Ghulam Abbas Kandhro,
Hassan Imran Afridi,
Naveed Kazi,
Jameel Ahmed Baig,
Mohammad Balal Arain,
Abdul Qadir Shah,
Nasreen Syed,
Sham Kumar,
Nida Fatima Kolachi,
Sumaira Khan
[show abstract]
[hide abstract]
ABSTRACT: In many developing countries, men and women are at high risk of goiter and iron deficiency. The aim of the recent study is
to assess the interaction of (Cu), with iron (Fe), iodine/iodide (I), and thyroid hormones in goitrous patients. Sixty goitrous
male (GMPs) and 72 female patients (GFPs) were evaluated for the Cu, Fe, I, and thyroid hormones status in biological samples
(serum and urine), and compared to non-goitrous subjects of both genders (M = 106, F = 120). The biological samples were analyzed
for Cu and Fe concentration using atomic absorption spectrometer, while I was measured by the potentiometric method, prior
to microwave-assisted acid digestion (MD). Quality control for the method was established with certified samples. Significantly
higher mean values of Cu in serum, and urine samples of GMPs and GFPs, while lower value of Fe and I were observed as compared
to control subjects (p < 0.015), respectively. The mean values of free triiodothyronine (FT3) and free thyroxin (FT4) were found to be lower in
goitrous patients of both genders than in the age-matched healthy controls (p < 0.006 and 0.002), respectively, in contrast high mean values of thyroid-stimulating hormone (TSH) were detected in patients
(p < 0.009), as compared to non-goitrous subjects. It was observed that the deficiencies of Fe, I, and thyroid hormone in goitrous
patients could be influenced by efficiency of Cu.
KeywordsCopper-Iron-Iodine-Thyroid hormones-Goiter
Biological Trace Element Research 04/2012; 134(3):265-279. · 1.92 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: ObjectiveThere is accumulating facts that the metabolism of essential trace elements is altered in diabetic patients. The aim of present
study was to compare the status of essential trace elements, chromium (Cr), manganese (Mn), and zinc (Zn) in biological samples
(whole blood, urine and scalp hair) of insulin dependent diabetic mothers (age ranged 30–40) and their newly born infants
(n=76). An age matched 68 non-diabetic mothers and their infants, residing in the same locality, were selected as referents.
For a comparative study, the biological samples of non-diabetic and diabetic pregnant and non pregnant of same age group and
socio-economics status were also analysed.
MethodologyThe biological samples (scalp hair, blood and urine) were collected from study and referent groups. The Cr, Mn and Zn concentrations
in all three biological samples were determined by a flame/electrothermal atomic absorption spectrometer, prior to microwave
assisted acid digestion. The validity and accuracy of the methodology was checked by certified reference materials (CRMs)
and using conventional wet acid digestion method on same CRMs.
ResultsThe mean values of Cr, Mn and Zn in scalp hair and blood samples of diabetic mothers and their infants were significantly
lower as compared to the referent mothers-infants pairs (p<0.01), while urinary excretion of all these elements were high in diabetic mother–infant pair samples.
ConclusionThe deficiencies of essential trace elements, Cr, Mn and Zn in biological samples of diabetic women, may play role in the
pathogenesis of diabetes mellitus and impacts on their neonates.
Archives of Gynecology and Obstetrics 04/2012; 280(3):415-423. · 1.28 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain,
Sirajuddin
[show abstract]
[hide abstract]
ABSTRACT: The most common cause of blindness in developing countries is vitamin A deficiency. The World Health Organization estimates
13.8 million children to have some degree of visual loss related to vitamin A deficiency. The causes of night blindness in
children are multifactorial, and particular consideration has been given to childhood nutritional deficiency, which is the
most common problem found in underdeveloped countries. Such deficiency can result in physiological and pathological processes
that in turn influence biological samples composition. Vitamin and mineral deficiency prevents more than two billion people
from achieving their full intellectual and physical potential. This study was designed to compare the levels of Zn, Mg, Ca,
K, Na, As, Cd, and Pb in scalp hair, blood, and urine of night blindness children age ranged 3–7 and 8–12years of both genders,
comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion procedure was developed as a sample
pretreatment, for the determination of As, Ca, Cd, K, Pb, Mg, Na, and Zn in biological samples of night blindness children.
The proposed method was validated by using conventional wet digestion and certified reference samples of hair, blood, and
urine. The concentrations of trace and toxic elements were measured by atomic absorption spectrophotometer prior to microwave-assisted
acid digestion. The results of this study showed that the mean values of As, Cd, Na, and Pb were significantly higher in scalp
hair, blood, and urine samples of male and female night blindness children than in referents (p < 0.001), whereas the concentrations of Zn, Ca, K, and Mg were lower in the scalp hair and blood but higher in the urine
samples of night blindness children. These data present guidance to clinicians and other professional investigating deficiency
of essential mineral elements in biological samples (scalp hair and blood) of night blindness children.
KeywordsNight blindness–Essential elements–Toxic elements–Biological samples–Wet acid digestion methods–Children–Age groups (3–12)–Atomic absorption spectrophotometer
Biological Trace Element Research 04/2012; 143(1):20-40. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi,
Sirajuddin, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain
[show abstract]
[hide abstract]
ABSTRACT: The causes of night blindness in children are multifactorial, and particular consideration has been given to childhood trace
metals toxicity, which is the most common problem found in underdeveloped countries. This study was designed to compare the
levels of cadmium (Cd), lead (Pb), and nickel (Ni) in scalp hair, blood, and urine of night blindness children age ranged
3–7 and 8–12years of both genders, comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion
procedure was developed as a sample pretreatment, for the determination of Cd, Pb, and Ni in biological samples of night blindness
children. The proposed method was validated by using conventional wet digestion and certified reference samples of hair, blood,
and urine. The digests of all biological samples were analyzed for Cd, Pb, and Ni by electrothermal atomic absorption spectrometry.
The results indicated significantly higher levels of Cd, Pb, and Ni in the biological samples (blood, scalp hair, and urine)
of male and female night blindness children, compared with control subjects of both genders. These data present guidance to
clinicians and other professional investigating toxicity of trace metals in biological samples of night blindness children.
KeywordsNight blindness–Cadmium–Lead–Nickel–Biological samples–Wet acid digestion methods–Children–Age groups (3–12)–Atomic absorption spectrophotometer
Biological Trace Element Research 04/2012; 142(3):350-361. · 1.92 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: In many developing countries, women are at high risk of goiter and iron deficiency anemia (IDA). Iron deficiency adversely
affects thyroid metabolism and may decrease the efficiency of thyroid hormones in areas of endemic goiter. The aim of the
present study was to compare the level of iron (Fe) in biological samples (serum and urine) and serum thyroid hormones, thyroid
stimulating hormone (TSH), free triiodothyronine (FT3), and free thyroxin (FT4) of goitrous female patients (GFPs; n = 69) with those of nongoitrous women as control subjects (n = 117), age range 21–45years. The biological samples were analyzed for Fe concentration using flame atomic absorption spectrophotometer,
prior to microwave-assisted wet acid digestion. The validity and accuracy of the method was checked by the certified sample
and with those obtained by conventional wet acid digestion method on the same CRM and real samples. The overall recoveries
of Fe in serum and urine were found in the range of 97.2–98.6% of certified values. The results of this study showed that
the mean values of Fe in serum and urine samples of GFPs were significantly reduced as compared to control subjects (p = 0.002 and p = 0.015, respectively). The mean values of FT3 and FT4 were found to be lower in GFPs than in the age-matched healthy control
women; in contrast, high mean values of TSH were detected in GFPs (p = 0.003). There was a positive correlation between serum Fe concentration and TSH (r = 0.85, p = 0.01), FT3 (r = 0.95, p = 0.003), and FT4 levels (r = 0.98, p = 0.007) in GFPs. It was observed that iron deficiency is prevalent in GFPs, so the need of Fe supplementation will be required
to improve the efficacy of thyroid metabolism in goitrous women.
Biological Trace Element Research 04/2012; 125(3):203-212. · 1.92 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: The determination of toxic metals (TMs) in the biological samples of human beings is an important clinical screening procedure.
The aim of this work is to determine total content of TMs, aluminum (Al), cadmium (Cd), nickel (Ni), and lead (Pb) in scalp
hair samples of chronic kidney male patients (CKPs) on maintenance hemodialysis, during the period of 2005–2007. The study
included 115 CKPs (all smokers) and 150 controls or referents [82 (nonsmokers) and 68 (smokers)]. Both controls and patients
(males) were of the same age group (ranged 25–55years), socioeconomic status, localities, and dietary habits. The scalp hair
samples were analyzed by electrothermal atomic absorption spectrometer, prior to microwave-induced acid digestion. The accuracy
of the total Al, Cd, Ni, and Pb measurements was tested by simultaneously analyzing certified reference material (human hair
NCS ZC81002). No significant differences were observed between the analytical results and the certified values (paired t test at p > 0.05). The levels of TMs in scalp hair samples of patients were found to be higher as compared to control nonsmoker and
smokers. Moreover, the study shows that levels of Al, Cd, Ni, and Pb in scalp hair samples may be useful to evaluate the impact
of cigarette smoking in kidney failure patients.
Biological Trace Element Research 04/2012; 127(1):16-27. · 1.92 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: In the present study, the association of arsenic, cadmium, lead, and nickel between scalp hair samples and mortality from
myocardial infarction (MI) patients at first, second, and third heart attack was studied. The biological samples of 130 MI
patients (77 male and 53 female) age ranged (45–60years), were collected and 61 healthy persons of same age group (33 male
and 28 female) was selected as control subjects. The toxic elements (TEs) in biological samples were assessed by the electrothermal
atomic absorption spectrophotometry prior to microwave-assisted acid digestion. The validity of methodology was checked by
the certified human hair reference material (BCR 397). It was observed during the study that 78% of 32 patients of the third
MI, age >50years were expired. In these subjects, the level of As, Cd, Ni, and Pb were increased by 10.6%, 19.5%, 15.7%,
and 9.8% in the scalp hair as compared to those who tolerated third MI attack (p = 0.12). The high level of toxic metals may play a role in the development of heart disease in the subjects of this study.
KeywordsToxic elements-Myocardial infarction-Scalp hair-Atomic absorption spectrophotometer
Biological Trace Element Research 04/2012; 134(1):1-12. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Gul Kazi,
Mohammad Khan Jamali,
Raja Adil Sarfaraz,
Mohammad Balal Arain, Ghulam Abbas Kandhro,
Abdul Qadir Shah,
Jamil Ahmed Baig,
Nusrat Jalbani,
Rehana Ansari
[show abstract]
[hide abstract]
ABSTRACT: There is accumulative evidence that the metabolism of iron and copper is altered in viral hepatic diseases, and these nutrients
might have specific roles in their pathogenesis and progress. The aim of present study was to compare the level of copper
(Cu) and iron (Fe) in biological samples (serum, urine, and scalp hair) of female patients suffering from different viral
hepatitis (A, B, C, D, and E; n = 253) of age range 31–45years. For comparative study, 95 healthy females of the same age group residing in the same city
were selected. The elements in the biological samples were analyzed by flame atomic absorption spectrophotometry, prior to
microwave-assisted acid digestion. The validity and accuracy of the methodology was checked by using certified reference materials
(CRMs) and with those values obtained by conventional wet acid digestion method on same CRMs. The results of this study showed
that the mean values of Cu and Fe were higher in sera and scalp hair samples of hepatitis patients than age-matched control
subjects, while the difference was significant (p < 0.001), in the cases of viral hepatitis B and viral hepatitis C as compared to viral hepatitis A, D, and E. The urinary
levels of these elements were found higher in the hepatitis patients than in the age-matched healthy controls (p < 0.05). These results are consistent with literature-reported data, confirming that hepatic iron and copper overload can
directly cause lipid peroxidation and eventually hepatic damage.
Biological Trace Element Research 04/2012; 129(1):78-87. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Abdul Qadir Shah,
Jameel Ahmed Baig,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain
[show abstract]
[hide abstract]
ABSTRACT: This study was designed to compare the levels of chromium (Cr) and manganese (Mn) in scalp hair, blood, and urine of night blindness in children age ranged (3-7) and (8-12) years of both genders, comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion procedure, was developed as a sample pretreatment, for the determination of Cr and Mn in biological samples of night blindness children. The proposed method was validated by using conventional wet digestion and certified reference samples of hair, blood and urine. The digests of all biological samples were analyzed for Cr and Mn by electrothermal atomic absorption spectrometry. The results indicated significantly higher levels of Cr, whilst low level of Mn in the biological samples (blood and scalp hair) of male and female night blindness children, compared with control subjects of both genders. These data present guidance to clinicians and other professional investigating deficiency of Mn and excessive level of Cr in biological samples (scalp hair and blood) of night blindness children.
Biological trace element research 10/2011; 143(1):103-15. · 1.92 Impact Factor
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Nida Fatima Kolachi,
Tasneem Gul Kazi,
Hassan Imran Afridi,
Naveed Kazi, Ghulam Abbas Kandhro,
Abdul Qadir Shah,
Jameel Ahmed Baig,
Sham Kumar Wadhwa,
Sumaira Khan,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain
[show abstract]
[hide abstract]
ABSTRACT: The aim of the present study was to compare the level of copper (Cu), iron (Fe) and zinc (Zn) in biological samples (serum, blood, urine, and scalp hair) of patients suffering from different viral hepatitis (A, B, C, D, and E; n = 521) of both gender age ranged 31-45 years. For comparative study, 255 age-matched control subjects, of both genders residing in the same city were selected as referents. The elements in the biological samples were analyzed by flame atomic absorption spectrophotometry, prior to microwave-assisted acid digestion. The validity and accuracy of the methodology was checked by using certified reference materials (CRMs) and with those values obtained by conventional wet acid digestion method on same CRMs. The results of this study showed that the mean values of Cu and Fe were higher in blood, sera, and scalp hair samples of hepatitis patients, while Zn level was found to be lower than age-matched control subjects. The urinary levels of these elements were found to be higher in the hepatitis patients than in the age-matched healthy controls (p < 0.05). These results are consistent with literature-reported data, confirming that the deficiency of zinc and hepatic iron and copper overload can directly cause lipid peroxidation and eventually hepatic damage.
Biological trace element research 10/2011; 143(1):116-30. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain,
Sirajuddin
[show abstract]
[hide abstract]
ABSTRACT: The most common cause of blindness in developing countries is vitamin A deficiency. The World Health Organization estimates 13.8 million children to have some degree of visual loss related to vitamin A deficiency. The causes of night blindness in children are multifactorial, and particular consideration has been given to childhood nutritional deficiency, which is the most common problem found in underdeveloped countries. Such deficiency can result in physiological and pathological processes that in turn influence biological samples composition. Vitamin and mineral deficiency prevents more than two billion people from achieving their full intellectual and physical potential. This study was designed to compare the levels of Zn, Mg, Ca, K, Na, As, Cd, and Pb in scalp hair, blood, and urine of night blindness children age ranged 3-7 and 8-12 years of both genders, comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion procedure was developed as a sample pretreatment, for the determination of As, Ca, Cd, K, Pb, Mg, Na, and Zn in biological samples of night blindness children. The proposed method was validated by using conventional wet digestion and certified reference samples of hair, blood, and urine. The concentrations of trace and toxic elements were measured by atomic absorption spectrophotometer prior to microwave-assisted acid digestion. The results of this study showed that the mean values of As, Cd, Na, and Pb were significantly higher in scalp hair, blood, and urine samples of male and female night blindness children than in referents (p < 0.001), whereas the concentrations of Zn, Ca, K, and Mg were lower in the scalp hair and blood but higher in the urine samples of night blindness children. These data present guidance to clinicians and other professional investigating deficiency of essential mineral elements in biological samples (scalp hair and blood) of night blindness children.
Biological trace element research 10/2011; 143(1):20-40. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi,
Sirajuddin, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah
[show abstract]
[hide abstract]
ABSTRACT: It has been speculated that trace elements may play a role in the pathogenesis of heart diseases In the present study, we aimed to access the levels of chromium (Cr) and manganese (Mn) in biological samples (whole blood, urine, and scalp hair) of myocardial infarction (MI) patients of both gender age ranged (45-60 years) at first, second, and third heart attack (n = 130), hospitalized in cardiac ward of National Hospital of Hyderabad city (Pakistan). For comparison, healthy age-matched referent subjects (n = 61), of both gender were also selected. The Cr and Mn in biological samples were measured by electrothermal atomic absorption spectrometry, prior to microwave-assisted acid digestion. The validity of the methodology was checked by the biological certified reference materials. During this study, 78% of 32 registered patients of third MI attack (aged >50 years) were died. In these subjects the concentration of Cr and Mn were decreased by 24.7% and 19.8% in scalp hair, while in blood samples 17.9% and 12.4%, respectively, as compared to those who tolerated third MI attack (p = 0.063). Although these data do not prove a causal relationship, these results are consistent with the hypothesis that heart disease may cause deficiencies of certain essential trace elements. The excretion levels of Cr and Mn in urine samples of first MCI were higher than controls at p values (0.029 and 0.011), respectively, whereas the excretion rates of both elements were further enhance after second myocardial infarction attack. The Cr and Mn concentration was inversely associated with the risk of myocardial infarction attacks in both genders. These results add to an increasing body of evidence that, Cr and Mn are importance for cardiovascular health.
Biological trace element research 09/2011; 142(3):259-73. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi,
Sirajuddin, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain
[show abstract]
[hide abstract]
ABSTRACT: The causes of night blindness in children are multifactorial, and particular consideration has been given to childhood trace metals toxicity, which is the most common problem found in underdeveloped countries. This study was designed to compare the levels of cadmium (Cd), lead (Pb), and nickel (Ni) in scalp hair, blood, and urine of night blindness children age ranged 3-7 and 8-12 years of both genders, comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion procedure was developed as a sample pretreatment, for the determination of Cd, Pb, and Ni in biological samples of night blindness children. The proposed method was validated by using conventional wet digestion and certified reference samples of hair, blood, and urine. The digests of all biological samples were analyzed for Cd, Pb, and Ni by electrothermal atomic absorption spectrometry. The results indicated significantly higher levels of Cd, Pb, and Ni in the biological samples (blood, scalp hair, and urine) of male and female night blindness children, compared with control subjects of both genders. These data present guidance to clinicians and other professional investigating toxicity of trace metals in biological samples of night blindness children.
Biological trace element research 09/2011; 142(3):350-61. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sumaira Khan,
Nida Fatima Kolachi,
Sham Kumar Wadhwa,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain
[show abstract]
[hide abstract]
ABSTRACT: Psoriasis is a noncontiguous common and chronic skin disorder. The aim of the present study was to compare the level of trace elements cadmium (Cd), chromium (Cr), Nickel (Ni), and zinc (Zn) in biological samples (whole blood, urine, and scalp hair) of psoriasis patients of both gender age ranged (25-55 years) at mild, moderate severe, and more severe stage (n = 418) living in the vicinity of cement factory. For comparison purposes, healthy age-matched referent subjects, residents of industrial and non-industrial area, of both gender were also selected (n = 241). The concentrations of trace and toxic elements were measured by atomic absorption spectrophotometer prior to microwave-assisted acid digestion. The validity and accuracy of methodology was checked by using certified reference materials (CRMs) and conventional wet acid digestion method on same CRMs and real samples. The results of this study showed that the mean values of Cd, Cr, Ni, and Pb were significantly higher in scalp hair, blood, and urine samples of mild and severe psoriasis patients as compared to referents (p < 0.001), while the concentration of Zn was lower in the scalp hair and blood, but higher in the urine samples of psoriasis patients. The deficiency of Zn in psoriasis patients may be undoubtedly caused by the toxic element exposures via cement factory.
Biological trace element research 09/2011; 142(3):284-301. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Abdul Qadir Shah,
Sham Kumar Wadhwa,
Sumaira Khan,
Nida Fatima Kolachi,
Faheem Shah,
Mohammad Khan Jamali,
Mohammad Balal Arain,
Sirajuddin
[show abstract]
[hide abstract]
ABSTRACT: The causes of night blindness in children are multifactorial, and particular consideration has been given to childhood nutritional deficiency, which is the most common problem found in underdeveloped countries. Such deficiency can result in physiological and pathological processes that in turn influence hair composition. This study was designed to compare the levels of zinc (Zn), copper (Cu), and iron (Fe) in scalp hair, blood, and urine of both genders of children with night blindness with age range of 3-7 and 8-12 years, comparing them to sex- and age-matched controls. A microwave-assisted wet acid digestion procedure was developed as a sample pretreatment, for the determination of zinc, copper, and iron in biological samples of children with night blindness. The proposed method was validated by using conventional wet digestion and certified reference samples of hair, blood, and urine. The digests of all biological samples were analyzed for Cu, Fe, and Zn by flame atomic absorption spectrometry using an air/acetylene flame. The results indicated significantly lower levels of Fe, Cu, and Zn in the biological samples (blood and scalp hair) of male and female children with night blindness, compared with control subjects of both genders. These data present guidance to clinicians and other professionals investigating the deficiency of essential trace metals in biological samples (scalp hair and blood) of children with night blindness.
Biological trace element research 09/2011; 142(3):323-34. · 1.92 Impact Factor
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Hassan Imran Afridi,
Tasneem Gul Kazi,
Naveed Kazi, Ghulam Abbas Kandhro,
Jameel Ahmed Baig,
Mohammad Khan Jamali,
Mohammad Balal Arain,
Abdul Qadir Shah,
Faheem Shah,
Sumaira Khan,
Nida Fatima Kolachi
[show abstract]
[hide abstract]
ABSTRACT: The exposure of toxic elements may directly or indirectly associate with different pathogenesis of heart diseases. In the present study, the association of arsenic (As), cadmium (Cd), cobalt (Co), lead (Pb), and nickel (Ni) in biological samples (whole blood and urine) and mortality from myocardial infarction (MI) patients at first, second, and third heart attacks was carried out. Both biological samples of 130 MI patients (77 male and 53 female), with ages ranging from 45 to 60 years, and 61 healthy persons (33 male and 28 female) of the same age group were collected. The elements in biological samples were assessed by electrothermal atomic absorption spectrophotometer, prior to microwave-assisted acid digestion. The validity of methodology was checked by the biological certified reference materials. During this study, 78% of 32 patients aged above 50 years, registered after third MI attack, died. In these subjects, the levels of As, Cd, Co, Ni, and Pb in blood samples were higher in MI patients as compared with referents (p < 0.05), while increased by 11.7%, 12.2%, 5.55%, and 7.2%, respectively, in the blood samples of those patients who tolerated the third MI attack (p = 0.12). The high level of understudied toxic elements may play a role in the mortality of MI patients.
Biological trace element research 06/2011; 141(1-3):26-40. · 1.92 Impact Factor
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ABSTRACT: The investigated data indicated that inorganic arsenic in drinking water is associated with increased mortality from different types of cancers. In the present study, biological samples (blood and scalp hair) of male subjects having lung and bladder cancers and non-cancerous subjects belonging to arsenic (As)-exposed area of southern parts of Pakistan were analysed for As contents. The As levels in drinking water of understudy area showed that sections of understudy population are exposed to arsenic concentrations, which was 3-15-fold higher than the permissible level (<10 μg/L). For comparative purposes the biological samples of matched male cancer patient, as referent patients belonging to big city (Hyderabad) who had used municipal treated water with low arsenic levels <10 μg/L, were also collected. The exposed cancer patients have 2-3-fold higher level of As in both biological samples compared to non-exposed case-matched cancerous male subjects. This study is compelling evidence in support of positive associations between arsenic-contaminated water, food and cigarette with different types of risks of cancer.
Human & Experimental Toxicology 05/2011; 30(12):2013-22. · 1.31 Impact Factor
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Biological trace element research 03/2011; 139(3):368. · 1.92 Impact Factor
