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ABSTRACT: This study was designed to examine the effects of vitamin E on the levels of Zn, Mn, Cu, Fe, and carbonic anhydrase in rats
with bleomycin-induced pulmonary fibrosis. Twenty-one male Wistar albino rats were randomly divided into three groups: bleomycin
alone, bleomycin+vitamin E, and saline alone (control group). The bleomycin group was given 7.5 mg/kg body weight (single
dose) bleomycin hydrochloride intratracheally. The bleomycin+vitamin E group was also instilled with bleomycin hydrochloride
but received injections of α-tocopherol twice a week. The control group was treated with saline alone. Animals were sacrified
14 d after intratracheal instillation of bleomycin. Tissue Zn, Mn, Cu, Fe, and carbonic anhydrase activities were measured
in the lung and liver. Lung Cu, Fe, and carbonic anhydrase activity increase in both experimental groups. Zn and Mn levels
decreased, except for the Mn level in the bleomycin group. Liver Zn, Mn, and Cu levels decreased in both experimental groups
compared to the control group, whereas Fe and carbonic anhydrase activity increased in comparison to the control group. However,
the liver tissue Fe level decreased compared to the control group. In the histopathologic assesment of lung sections in the
bleomycin+vitamin E group, partial fibrotic lesions were observed, but the histopathologic changes were much less severe compared
to the bleomycin-treated group.
Biological Trace Element Research 04/2012; 116(3):289-300. · 1.92 Impact Factor
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ABSTRACT: The combined effects of vitamin E and selenium were studied in native Anatolian horses subject to strenuous exercise. The concentrations of copper, zinc, iron, calcium, potassium, and magnesium were determined in serum by atomic absorption spectrometry in two study groups (n = 25 each), one of which served as untreated controls. After exercising the horses by running 1,500 m in about 7 min, only the copper level and the copper/zinc ratio significantly increased (p < 0.05), but the concentrations of calcium, potassium, iron, and magnesium remained unchanged. In horses treated with vitamin E and selenium, the calcium and potassium levels decreased to levels lower than those of untreated controls before and after exercise. The iron levels were not changed by exercise or treatment alone but increased when the horses had been supplemented and exercised. The copper level and the copper/zinc ration increased as a result of exercise in both treated and untreated horses. These changes suggest that supplementation with vitamin E and selenium had an important effect on the serum concentrations of calcium, potassium, copper, iron, and the copper/zinc ratio.
Biological trace element research 10/2008; 125(3):223-8. · 1.92 Impact Factor
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ABSTRACT: The aim of this study was to determine the serum concentrations of selenium, vitamin E, and total- and lipid-bound sialic acid (LBSA) in lambs with white muscle disease (WMD) before and after treatment with a commercial preparation containing selenite and vitamin E. Fifteen lambs with WMD and ten control animals were used as research materials. Blood samples were collected from both groups before- and 1 month after treatment for Se analysis by fluorimetry, whereas vitamin E and sialic acid were measured by HPLC and spectrophotometry, respectively. Compared to controls, in the diseased animals, there was a significant increase of serum total sialic acid (TSA) and LBSA, together with significant decreases of serum Se and vitamin E concentrations (p < 0.001). One month after treatment, a reversal of trend was observed with decreases of TSA and LBSA and increases of Se and vitamin E concentrations. The TSA and LBSA levels, however, remained significantly higher than those of the controls, p < 0.05 and 0.001, respectively. The Se and vitamin E concentrations of the treated animals were the same as those of controls. This is the first study on total and LBSA concentrations in lambs with WMD, showing that these markers can be used in the prognosis of the disease.
Biological Trace Element Research 01/2008; 121(1):39-43. · 1.92 Impact Factor
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ABSTRACT: ZET Bu çalışma, Babesia ovis ile doğal olarak enfekte olan koyunlarda serum folik asit düzeylerinin araştırılması ve tedavi sonrasında nasıl etkilendiklerinin saptanması amacıyla yapıldı. Klinik ve parazitolojik olarak babesiosis tanısı konulan 30 adet koyun hasta grubunu, 10 adet sağlıklı koyun da kontrol grubunu oluşturdu. Hasta koyunlar, antiprotozoon ilaç (tek dozda diminazene aceturate'ın % 7 lik solüsyonu, i.m.) verilerek tedavi edildi. Tedavinin 7. gününde tekrar bu gruptan kan örnekleri toplandı. Yapılan analizlerde serum folik asit düzeylerinin hasta grupta en düşük olduğu (p<0.05), tedavi ile birlikte bu düzeylerin hastaya göre önemli oranda arttığı (p<0.05), ancak yine de kontrol grubuna göre önemli oranda azalmış olduğu tespit edildi. Sonuç olarak B. ovis enfeksiyonun serum folik asit düzeylerinin önemli oranda düşmesine yol açtığı, tedaviyle birlikte bir miktar arttığı görüldü. Anahtar Kelimeler Babesia ovis, Koyun, Folik asit The Serum Folic acid concentrations in Sheep with Naturally Infected with Babesia ovis SUMMARY The aim of this study was to determine the serum concentrations of Folic acid in sheep naturally infected with Babesia ovis before and after treatment. Thirty diseased sheep and ten control animals were included in the present study. Compared to the control animals the serum folic acid concentrations were lower, after treatment with antiprotozootic agent, the folic acid levels increased, but, these increasing levels were still lower than control group.
YYÜ Vet Fak Derg. 01/2008;
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ABSTRACT: This study was designed to examine the effects of vitamin E on the levels of Zn, Mn, Cu, Fe, and carbonic anhydrase in rats with bleomycininduced pulmonary fibrosis. Twenty-one male Wistar albino rats were randomly divided into three groups: bleomycin alone, bleomycin+vitamin E, and saline alone (control group). The bleomycin group was given 7.5 mg/kg body weight (single dose) bleomycin hydrochloride intratracheally. The bleomycin+vitamin E group was also instilled with bleomycin hydrochloride but received injections of alpha-tocopherol twice a week. The control group was treated with saline alone. Animals were sacrified 14 d after intratracheal instillation of bleomycin. Tissue Zn, Mn, Cu, Fe, and carbonic anhydrase activities were measured in the lung and liver. Lung Cu, Fe, and carbonic anhydrase activity increase in both experimental groups. Zn and Mn levels decreased, except for the Mn level in the bleomycin group. Liver Zn, Mn, and Cu levels decreased in both experimental groups compared to the control group, whereas Fe and carbonic anhydrase activity increased in comparison to the control group. However, the liver tissue Fe level decreased compared to the control group. In the histopathologic assesment of lung sections in the bleomycin+vitamin E group, partial fibrotic lesions were observed, but the histopathologic changes were much less severe compared to the bleomycin-treated group.
Biological Trace Element Research 07/2007; 116(3):289-300. · 1.92 Impact Factor
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ABSTRACT: The study was undertaken to investigate the influence of alpha-tocopherol on zinc, copper, iron, calcium, magnesium, and potassium concentrations in serum of rats with bleomycin-induced pulmonary fibrosis. Fourteen Wistar albino rats were randomly divided into two groups of seven animals each. The first group was treated intratracheally with bleomycin hydrochloride (BM group); the second group was also instilled with BM but received injections of alpha-tocopherol twice a week (BM+E group). The third group was treated in the same manner with saline solution only, acting as controls (C). The zinc concentrations of the BM and BM+E groups were significantly decreased compared to the controls (p<0.05). The iron concentration of the controls was significantly higher than the other two groups. The magnesium concentration in the controls and the BM+E group was significantly higher than that of the BM group. The serum copper, calcium, and potassium concentrations were not found to be statistically different among the three groups. Distinct histopathologic changes were found in the BM group compared to the untreated rats. Less severe fibrotic lesions were also observed in the BM+E group. The results of this study show that lungs of rats treated with bleomycin were seriously damaged and that vitamin E seemed to counteract some of the damage, as indicated by differences in the serum concentrations of major elements.
Biological Trace Element Research 02/2006; 114(1-3):175-84. · 1.92 Impact Factor
