Charles Mutai

Publications (6)9.47 Total impact

• Source

  Available from: Pascaline Jeruto

  Article: Effects of Triterpenoids on Herpes Simplex Virus Type1 (Hsv-1) In Vitro

  Charles Mutai, Lucia Keter, Lilian Ngeny, Pascaline Jeruto
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  ABSTRACT: Citation: Mutai C, Keter L, Ngeny L, jeruto P (2012) Effects of Triterpenoids on Herpes Simplex Virus Type1 (Hsv-1) In Vitro. Med Aromat Plants 1:106.
  med. Aromat pts. 06/2012; 1(5-1:106).
• Source

  Available from: ajol.info

  Article: In vitro effects of Warburgia ugandensis, Psiadia punctulata and Chasmanthera dependens on Leishmania major promastigotes.

  Edward K Githinji, Lucy W Irungu, Willy K Tonui, Geoffrey M Rukunga, Charles Mutai, Charles N Muthaura, Reuben Lugalia, Geoffrey Gikandi, Caroline W Wainaina, Johnston M Ingonga, Antony Wanjoya
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  ABSTRACT: Plant extracts from Warburgia ugandensis Sprague (Family: Canellaceae), Psiadia punctulata Vatke (Family: Compositae) and Chasmanthera dependens Hoschst (Family: Menispermaceae) were tested for activity on Leishmania major promastigotes (Strain IDU/KE/83 = NLB-144) and infected macrophages in vitro. Plants were collected from Baringo district, dried, extracted, weighed and tested for antileishmanial activity. Serial dilutions of the crude extracts were assayed for their activity against Leishmania major in cell free cultures and in infected macrophages in vitro. Inhibitory concentrations and levels of cytotoxicity were determined. Warburgia ugandensis, Psiadia punctulata and Chasmanthera dependens had an IC(50) of 1.114 mg/ml, 2.216 mg/ml and 4.648 mg/ml, respectively. The cytotoxicity of the drugs on BALB/c peritoneal macrophage cells was insignificant as compared to the highly toxic drug of choice Pentostam(®). The supernatants from control and Leishmania infected macrophages were analyzed for their nitrite contents by Griess reaction and nitrite absorbance measured at 540 nm. Warburgia ugandensis (stem bark water extract), Chasmanthera dependens (stem bark water extract) and Psiadia punctulata (stem bark methanol extract) produced 112.3%, 94% and 88.5% more nitric oxide than the untreated infected macrophages respectively. Plant crude extracts had significant (p<0.05) anti-leishmanial and immunomodulative effects but insignificant cytotoxic effects at 1mg/ml concentration. All experiments were performed in triplicate. Statistical analysis of the differences between mean values obtained from the experimental group compared to the controls was done by students't test. ANOVA was used to determine the differences between the various treatment groups. The analysis program Probit was used to determine IC(50)s.
  African Journal of Traditional, Complementary and Alternative Medicines 01/2010; 7(3):264-75. · 0.71 Impact Factor
• Article: Antimicrobial activity of Acacia mellifera extracts and lupane triterpenes.

  Charles Mutai, Christine Bii, Constantinos Vagias, Dennis Abatis, Vassilios Roussis
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  ABSTRACT: Acacia mellifera (Vahl) Benth (Leguminosae) is a subtropical medicinal plant that is widely used in traditional African medicines against various diseases such as pneumonia and malaria. The present study was performed to evaluate the antimicrobial effects of extracts from the stem bark of Acacia mellifera. The extracts were examined for antibacterial and antifungal activity using the disc-diffusion method against bacterial strains: Streptococcus pneumoniae (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 35218), Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) and fungal strains Candida albicans (ATCC 90028), Candida krusei (ATCC 6255), Cryptococcus neoformans (ATCC 6603) and clinical isolates of Microsporum gypseum and Trichophyton mentagrophytes. Some of these extracts were found to be active against some bacterial and fungal strains and were further fractionated to give 12 pure compounds. The methanolic and methanol:dichloromethane (1:1) extracts exhibited antibacterial and antifungal activity. The two were active against Staphylococcus aureus (ATCC 25923), Microsporum gypseum, and Trichophyton mentagrophytes. Activity guided fractionation led to isolation of two active compounds: 3-(Z)-cis coumaroylbetulin and 30-hydroxylup-20 (29)-en-3beta-ol which were against Staphylococcus aureus (ATCC 25923), Microsporum gypseum, Trichophyton mentagrophytes and Pseudomonas aeruginosa (ATCC 27853). These results may partly explain and support the use of Acacia mellifera stem barks for the treatment of infectious diseases in traditional Kenya medicine.
  Journal of ethnopharmacology 06/2009; 123(1):143-8. · 2.32 Impact Factor
• Source

  Available from: Dimitri Moreau

  Article: Lupane triterpenoids from Acacia mellifera with cytotoxic activity.

  Charles Mutai, Dennis Abatis, Constantinos Vagias, Dimitri Moreau, Christos Roussakis, Vassilios Roussis
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  ABSTRACT: Three new pentacyclic triterpenoids: (20R)-3-oxolupan-30-al (1), (20S)-3-oxolupan-30-al (2) and (20R)-28-hydroxylupen-30-al-3-one (3), along with (20S)-3beta-hydroxylupan-30-al (4), the latter previously described as a constituent of an epimeric mixture, were isolated from Acacia mellifera. In addition, the known metabolites 30-hydroxylup-20-(29)-en-3-one (5), 30-hydroxylup-20-(29)-en-3beta-ol (6), atranorin, methyl 2,4-dihydroxy-3,6 dimethyl benzoate, sitosterol-3beta-O-glucoside and linoleic acid were found in the analyzed plant species for the first time. The structures of the new metabolites were elucidated by extensive spectroscopic analyses and their relative stereochemistry was determined by NOESY experiments. The new metabolite 3 exhibited significant cytotoxic activity against the NSCLC-N6 cell line, derived from a human non-small-cell bronchopulmonary carcinoma.
  Molecules 02/2007; 12(5):1035-44. · 2.39 Impact Factor
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  Available from: ajol.info

  Article: In vivo screening of antimalarial activity of Acacia mellifera (Benth) (Leguminosae) on Plasmodium berghei in mice.

  Charles Mutai, Geoffrey Rukunga, Constatinos Vagias, Vassilios Roussis
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  ABSTRACT: The activities of total extracts and lupane triterpenes, isolated from the stem bark of Acacia mellifera, were evaluated against Plasmodium berghei strain ANKA in a female Swiss mouse model. Five isolated compounds and the crude extracts were evaluated for antimalarial activity and Quinine hydrochloride was used as a positive control. Only betulin and the methanolic extract produced considerable antimalarial activity in mice infected with P. berghei parasites. This study demonstrated the presence of bioactive agents in Acacia mellifera.
  African Journal of Traditional, Complementary and Alternative Medicines 01/2007; 5(1):46-50. · 0.71 Impact Factor
• Article: Cytotoxic lupane-type triterpenoids from Acacia mellifera.

  Charles Mutai, Dennis Abatis, Constantinos Vagias, Dimitri Moreau, Christos Roussakis, Vassilios Roussis
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  ABSTRACT: One new and eight previously described lupane-type metabolites were isolated for the first time from Acacia mellifera (Leguminosae). Based on spectral analyses, the structure of the new compound was elucidated as 28-hydroxy-3-oxo-lup-20-(29)-en-30-al (1), while the known compounds were identified as 3-oxo-lup-20-(29)-en-30-al (2), 3-hydroxy-lup-20-(29)-en-30-al (3), 28-hydroxy-lup-20-(29)-en-3-one (4), lupenone (5), lupeol (6), betulin (7), betulinic acid (8), and betulonic acid (9). Metabolites 2, 3, and 4 are reported for the first time in the Leguminosae family. The cytotoxicity of the isolated metabolites was evaluated on the NSCLC-N6 cell line, derived from a human non-small-cell bronchopulmonary carcinoma. Compounds 1 and 3 exhibited significant levels of activity.
  Phytochemistry 05/2004; 65(8):1159-64. · 3.35 Impact Factor