Mariana Carmen Chifiriuc

University of Bucharest, Bucureşti, Bucureşti, Romania

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Publications (174)203.51 Total impact

  • Lazar Veronica · Crina-Maria Saviuc · Mariana Carmen Chifiriuc ·
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    ABSTRACT: Oral microbiota of the mouth is the most diverse microbial community in the human body and plays a decisive role in the emergence and evolution of gingival pathology, contributing as well to the host general health condition, based on complex interactions established between the microbial community members and the host. A specific shift in the quantity and diversity of the microbial community developed on dental and mucosal surfaces, could lead to the occurrence of chronic inflammation mediated by the overproduction of pro-inflammatory cytokines. The mechanical treatment and current medication efficiency for the periodontal disease is limited in time due to the rapid plaque forming. Also, the antimicrobial treatment is limited by the sessile growth of the microorganisms, resulting in a poor biofilm penetration by biocides or antibiotics. In line with that, the attention of the scientific community shifted to ethnopharmacology as a complementary, or alternative therapeutic option for fighting infections with resistant bacteria. The vegetal and bee products are an important source of bioactive compounds, acting as harmless antimicrobials and periodontal inflammation suppressors. Vegetable bioproducts have been proven to exhibit multiple antipathogenic effects, such as microbicidal activity, virulence attenuation, and synergistic effects between the components found in the complex vegetal matrixes, or with conventional biocides, as well as immunomodulatory effects. The purpose of this review is to highlight the importance of vegetable products as a possible complementary treatment for periodontitis and their potential for the development of innovative therapeutic strategies.
    Current pharmaceutical design 11/2015; · 3.45 Impact Factor

  • Applied Surface Science 11/2015; DOI:10.1016/j.apsusc.2015.10.183 · 2.71 Impact Factor
  • Alina Maria Holban · Coralia Bleotu · Mariana Carmen Chifiriuc · Veronica Lazar ·
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    ABSTRACT: The objective of this study was to investigate the effects of P. aeruginosa PAO1 cellular and soluble culture fractions on human mesenchymal stem cells (MSCs) death signaling pathways and cytokine profile. The bone marrow isolated MSCs, incubated for different periods of time with one of the three P. aeruginosa PAO1 culture fractions, i.e. low density whole cultures, heat inactivated bacterial cultures sediments and sterile supernatants, were submitted to the following assays: i) fluorescence microscopy evaluation of cellular morphology and viability; ii) bax, caspase 9, relA and bcl-2 genes expression analysis by qRT-PCR; iii) quantification of the level of IL-1β, IL-6, IL-8 and IL-10 cytokines released in the MSCs supernatants determined by ELISA. Results were statistically analyzed using the GraphPad In Stat software. The PAO1 whole cultures exhibited the most relevant influences, impacting on MSCs morphology and viability, interfering with apoptotic pathways and significantly stimulating the production of IL-1β and IL-10, while decreasing the production of IL-6 and IL-8. The culture supernatants increased the production of IL-1β and reduced the secretion of all other tested cytokines, while heat-inactivated bacterial cells significantly stimulated both IL-1β and IL-10 production. These data could suggest that in vivo, the fate of P. aeruginosa infection depends on the proportion between different bacterial culture fractions (i.e. the number of viable bacterial cells, the number of dead cells and the amount of bacterial soluble products accumulated locally) that could be influenced by the initial infective dose, by the host defence mechanisms, and also by the administered antimicrobial treatment that may thus interfere with the evolution and magnitude of the induced lesions.
    Current Stem Cell Research & Therapy 10/2015; · 2.21 Impact Factor
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    ABSTRACT: The essential oil extracted by hydrodistillation from Romanian Artemisia annua aerial parts was characterized by GC/MS analysis, which allowed the identification of 94.64% of the total oil composition. The main components were camphor (17.74%), α-pinene (9.66%), germacrene D (7.55%), 1,8-cineole (7.24%), trans-β-caryophyllene (7.02%), and artemisia ketone (6.26%). The antimicrobial activity of this essential oil was evaluated by determining the following parameters: minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), minimal fungicidal concentration (MFC), and minimal biofilm eradication concentration (MBEC). Moreover, the soluble virulence factors were quantified with different biochemical substrates incorporated in the culture media. The reference and resistant, clinical strains proved to be susceptible to the A. annua oil, with MICs ranging from 0.51 to 16.33mg/ml. The tested essential oil also showed good antibiofilm activity, inhibiting both the initial stage of the microbial cell adhesion to the inert substratum and the preformed mature biofilm. When used at subinhibitory concentrations, the essential oil proved to inhibit the phenotypic expression of five soluble virulence factors (hemolysins, gelatinase, DNase, lipases, and lecithinases). Briefly, the present results showed that the A. annua essential oil contained antimicrobial compounds with selective activity on Gram-positive and Gram-negative bacterial strains as well as on yeast strains and which also interfere with the expression of cell-associated and soluble virulence factors.
    Chemistry & Biodiversity 10/2015; 12(10):1554-1564. DOI:10.1002/cbdv.201400340 · 1.52 Impact Factor

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    ABSTRACT: Here we report a newly synthesized vectorizing nanosystem, based on hydrodispersible magnetite nanoparticles (HMNPs) with an average size less than 10 nm, obtained by precipitation of Fe(II) and Fe(III) in basic solution of p-aminobenzoic acid (PABA), characterized by high-resolution transmission electron microscopy (HR-TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), differential thermal analysis coupled with thermogravimetric analysis (DTA-TGA) and bioevaluated for cytotoxicity and antibiotic delivery in active forms. The obtained data demonstrate that HMNPs can be used as an efficient drug delivery system, for clinically relevant antimicrobial drugs. HMNPs antimicrobial activity depended on the loaded drug structure and the tested microbial strain, being more efficient against Pseudomonas aeruginosa, comparing with the Escherichia coli strain. The novel HMNPs demonstrated an acceptable biocompatibility level, being thus a very good candidate for biomedical applications, such as drug delivery or targeting.
    Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 07/2015; 56(2):365-70. · 0.66 Impact Factor
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    ABSTRACT: The purpose of this study was to investigate the response of HeLa cells to the interaction with inactivated Staphylococcus aureus cells and live challenge with herpes simplex virus (HSV).The results of this study are indicating that the interaction between the HeLa cells and S. aureus inactivated whole cells could modulate the host cell apoptosis and cytokine production, and therefore, influence the progression of HSV infection. The pre-treatment of HeLa cells with heat inactivated bacterial whole cells protects them from the occurrence of HSV mediated cytopathic effect, while the post viral infection treatment with bacterial cells prevents the high activation of bax÷bcl-2 apoptotic pathway, a process that could change the fate of the infectious process triggered by the virus, and eventually reduce its multiplication rate. The pre-treatment of HeLa monolayer with inactivated bacterial cells 24 hours before the viral infection is increasing the expression level of TNF-a, IL-6 and IL-8 pro-inflammatory cytokines genes, also suggesting that bacterial antigens could contribute to the decrease of viral multiplication rate.
    Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 07/2015; 56(2):433-7. · 0.66 Impact Factor

  • 12th International Conference on Nanosciences & Nanotechnologies (NN15) 7-10 July 2015, Thessaloniki, Greece, Thessaloniki, Greece; 07/2015

  • 12th International Conference on Nanosciences & Nanotechnologies – NN15, Thessaloniki, Greece; 07/2015
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    ABSTRACT: The continuously spreading use of implantable medical devices (IMDs) in all fields of medicine increased the risk of microbial colonization leading to difficult to treat infections. The objective of this study was to obtain and characterize by physico-chemical and biological methods, new biopolymeric composites with anti-adherence properties based on polycaprolactone (PCL) dopped with chitosan (CS) and essential oils (EOs) as antimicrobial agents, for further use in the manufacture or coating of IMDs. The EOs were extracted from cinnamon (CIN), coriander (COR) and tea tree (TEA), and were characterized by GC-MS. Biopolymeric composite films (PCL/CS, PCL/CS/EOs) were obtained by solvent evaporation in two variants and further characterized by DSC and FTIR techniques. The in vitro antimicrobial activity of EOs was determined by the minimum inhibitory concentration (MIC) assay in liquid medium, towards Gram-positive and Gram-negative bacterial strains. The anti-adherence activity of films was analyzed against Staphylococcus aureus ATCC 29213 by viable cell counts assay. The cytotoxicity of the obtained films was assessed using the HT29 cell line. The results of this study suggest that CIN EO strongly inhibited the growth of all tested bacterial species, followed by TEA and COR EOs. From all tested composites, only PCL/CS/CIN 1 exhibited a significant anti-adherence effect.
    Romanian Biotechnological Letters 07/2015; 20(3):10521-10535. · 0.40 Impact Factor
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    ABSTRACT: This study reports the synthesis of silver nanoparticles (Ag NPs) by the sodium borohydride reduction method and their embedding in a silica shell functionalized with several antibiotics (ampicillin, penicillin G and isoniazide). The characterization of the novel materials was made by UV-visible absorption spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, dynamic light scattering and thermal analysis. TEM micrographs revealed the formation of spherical nanoparticles with size ranging between 20 and 50 nm. The Ag NPs embedded in the silica network exhibited a good antimicrobial efficiency, comparable or even superior to that of antibiotic-containing formulations. This is the first report regarding the synergic antimicrobial effect of Ag NPs embedded in silica and ampicillin, both against planktonic cells and biofilms formed by P. aeruginosa and S. aureus, two of the most fearful resistant bugs. These results are demonstrating the great potential of these nanocomposites to be used in developing novel antimicrobial agents or improving the existent ones, by increasing their efficiency, extending their spectrum of activity and decreasing the probability to select resistance, by simultaneously targeting multiple targets in the microbial cells.
    Journal of Inorganic and Organometallic Polymers and Materials 07/2015; 25(4). DOI:10.1007/s10904-015-0176-7 · 1.16 Impact Factor
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  • A. M. Holban · M. C. Chifiriuc · A. M.Grumezescu · I. Florin · E. Andronescu · V. Lazar ·
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    ABSTRACT: The purpose of this study was to develop a biocompatible nanomaterial based on magnetite and plant-derived compounds, such as eugenol and limonene in order to alter biofilm development and decrease resistance and persistence of the opportunistic pathogen Pseudomonas aeruginosa. This study used five clinical and one reference P. aeruginosa strains. Functionalized magnetite nanoparticles (Fe3O4@) have been synthesized by precipitation method, characterized by IR microscopy, SEM and TEM and functionalized with eugenol and limonene. The minimum inhibitory concentrations and biofilm formation were assessed by microdilution method followed by viable count. The impact of the obtained nanosystems on P. aeruginosa resistance and persisters formation was assessed for both planktonic cultures and biofilm embedded bacteria after treatment with the antibiotics gentamicin and norfloxacin. The biocompatibility of these nanostructures was assessed in vitro by fluorescence microscopy and MTT assay, using human cultured endothelial cells. Our data demonstrated that attachment and biofilm formation is significantly altered in all tested P. aeruginosa strains in a dose and strain dependent manner. Results revealed that functionalized magnetite nanostructures alter the percentage of persisters development after the antibiotics treatment with at least two fold for Fe3O4@ limonene and three fold for of Fe3O4@ eugenol, as compared with the situation in which bacteria are grown in the absence of the prepared nanosystems. These nanomaterials proved a good cytocompatibility in vitro. These results contribute to the development of modern therapies based on bioactive nanoparticles to fight resistant infections through modulating biofilm formation and persistence of the highly resistant pathogen P. aeruginosa.
    40th FEBS Congress, The Biochemical Basis of Life, Pages 154; 06/2015
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    ABSTRACT: The goal of this study was to obtain at low temperature a functional nano-composite with characteristics similar to the natural bone by using a cost effective method. The structure and morphology of collagen coated zinc doped hydroxyapatite bio-composites (Zn:HApCBc) were examined by X-Ray diffraction (XRD) and Scanning Electron Microscopy (SEM). XRD analysis revealed that the unique hexagonal Ca10(PO4)6(OH)2 in P63m space group was observed in the obtained nanocomposites Zn:HAp-CBc. The cytotoxicity of the Zn:HAp-CBc was studied on HeLa cell lines. Cell cycle distribution after treatment was examined by flow cytometry analysis. Our preliminary in vitro studies revealed that the obtained composites based on Zn doped HAp embedded in collagen matrix have excellent biocompatibility and support their further characterization by in vivo approaches and development as a biomaterial used in bone regeneration.
    Digest Journal of Nanomaterials and Biostructures 06/2015; Vol. 10(No.2):p. 681-691. · 0.95 Impact Factor
  • L Floroian · C Samoila · M Badea · D Munteanu · C Ristoscu · F Sima · I Negut · M C Chifiriuc · I N Mihailescu ·
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    ABSTRACT: A solution is proposed to surpass the inconvenience caused by the corrosion of stainless steel implants in human body fluids by protection with thin films of bioactive glasses or with composite polymer-bioactive glass nanostructures. Our option was to apply thin film deposition by matrix-assisted pulsed laser evaporation (MAPLE) which, to the difference to other laser or plasma techniques insures the protection of a more delicate material (a polymer in our case) against degradation or irreversible damage. The coatings composition, modification and corrosion resistance were investigated by FTIR and electrochemical techniques, under conditions which simulate their biological interaction with the human body. Mechanical testing demonstrates the adhesion, durability and resistance to fracture of the coatings. The coatings biocompatibility was assessed by in vitro studies and by flow cytometry. Our results support the unrestricted usage of coated stainless steel as a cheap alternative for human implants manufacture. They will be more accessible for lower prices in comparison with the majority present day fabrication of implants using Ti or Ti alloys.
    Journal of Materials Science Materials in Medicine 06/2015; 26(6):5527. DOI:10.1007/s10856-015-5527-y · 2.59 Impact Factor
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    ABSTRACT: the molecular interactions between the opportunistic pathogen Pseudomonas aeruginosa and the model organism Drosophila melanogaster (fruit fly), highlighting the impact of this interaction on the transcription rate of several bacterial Quorum sensing (QS) genes. Materials and methods. For the experimental infection, three distinct biological replicates of wild-type (Oregon) and respectively mutant gammaCop14a/gammaCop14a homozygous young D. melanogaster males were used. The flies were feed with 230 μl of sucrose suspension containing 9 X 109 CFU of P. aeruginosa ATCC 27853. We used sterile sucrose instead of bacterial suspension for feeding an equal number of control replicates for both considered male categories. The flies were maintained in standard conditions for approximately 60h prior to sample collection. The expression of the main genes involved in QS modulation in P. aeruginosa (lasI, lasR, rhlI, rhlR) was analyzed by qRT-PCR using cDNA obtained from the biological replicates of Oregon infected, Oregon control, gammaCop14a infected and respectively gammaCop14a control males. For each cDNA we performed two technical replicates with specific TaqMan probes. RplU gene was used as the endogen normalizer. Statistical analysis was performed using Student’s t-test ran on GraphPad Prism v5 software. Results. Our previous data revealed that ingested P. aeruginosa significantly modulates the composition of cultivable microbiota in both D. melanogaster male categories, suggesting the occurrence of QS mediated interactions. In the current study, when control flies were subjected to qRT-PCR analyses, we did not obtain any amplification signal, an expected result since they do not normally have a significant P. aeruginosa bacterial load. Considering the infected flies, we amplified P. aeruginosa QS related genes and investigated if we can discriminate between the expression levels in different backgrounds. We found that neither with complete Ct data nor when outliers were eliminated the genes lasI, lasR and rhl-R did not show a significant variation when comparing Oregon and mutant males. Only when data without outliers were employed, we were able to calculate significant transcripts level differences of rhl-I gene between wild-type and gammaCop mutant individuals (P = 0.0083). These data indicate a role for the specific host immune response and of genetic background of flies in modulating the virulence regulator rhl-I of P. aeruginosa. Conclusions. This study could be considered as a starting point for deciphering the implication of D. melanogaster innate immune system in the bacterial QS modulation and brings new insights in the elucidation of molecular mechanisms of host-pathogen communication. This work was supported by the grant IDEAS, no. 154/2011.
    ECCMID 2015, Copnehagen; 04/2015
  • O. Banu · I. Gheorghe · I. Czobor · L. Marutescu · A.I. Cotar · M. Mihai · A.M. Holban · V. Lazar · M.C. Chifiriuc ·
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    ABSTRACT: OBJECTIVES: Methicillin resistance is conferred by the acquisition of the mecA gene, which is carried by a mobile genetic element called the staphylococcal cassette chromosome mec (SCCmec). There are five major types of SCCmec elements (I-V). The present study was aimed to identify the types of SCCmec among clinical S. aureus isolates from different clinical specimens. METHODS: This study was conducted on a total of 83 S. aureus clinical strains isolated during 2014, out of which 40 from chronic wounds (venous and arterial ulcers, pressure sores, thoracic chronic ulcers secondary to breast cancer, nonhealing surgical wounds and abscesses) and 43 from clinical specimens (blood cultures, nasal exsudates, surgical wound secretions, catheters) taken from patients hospitalized for cardiovascular surgery harboring infections with different localizations. Singleplex and multiplex PCR was performed on genomic DNA from MSSA and MRSA isolates in order to identify the Staphylococcal Cassette Chromosome mec (SCCmec) central elements-ccr and mec complex and to establish the SCCmec type (type I, II, III, IVa-d, V). RESULTS The incidence of MRSA was significantly higher in Staphylococcus aureus strains isolated from patients with cardiovascular surgery (90% vs 30% in case of those isolated from chronic wounds). The MRSA phenotype was confirmed by the presence of mecA gene only in case of 11% of the MRSA strains isolated from chronic wounds and in 50% of those isolated from patients with cardiovascular surgery. The inducible (MLSBi) and constitutive macrolide (MLSBc) resistance was also more frequently encountered among the S. aureus strains from the first group (30% versus 0% for MLSBi and 5% versus <1% for MLSBc). The mecIVa was the most frequent type of the S. aureus strains isolated from infections occured in patients with cardiovascular surgery (44.18%), irrespective to the localization of the infectious process and the analyzed clinical sample, followed by the far less frequent type III (4.65%) and type V (2.32%). In case of MRSA strains isolated from chronic wounds, only the types III and V were encountered. However, a significant number of the MRSA strains from the both groups were nontypeable. CONCLUSION: These results confirm the high prevalence of SCCmec IVa, usually community-acquired, among the MRSA strains isolated from patients with cardiovascular surgery associated infections and the prevalence od SCCmec types III and V in MRSA strains colonizing hospitalized patients with chronic wounds.
    ECCMID, Copenhagen; 04/2015
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    ABSTRACT: The majority of chronic infections are associated with mono- or polymicrobial biofilms, having a significant impact on the patients' quality of life and survival rates. Although the use of medical devices revolutionized health care services and significantly improved patient outcomes, it also led to complications associated with biofilms and to the emergence of multidrug resistant bacteria. Immunocompromised patients, institutionalized or hospitalized individuals, elderly people are at greater risk due to life-threatening septic complications, but immunocompetent individuals with predisposing genetic or acquired diseases can also be affected, almost any body part being able to shelter persistent biofilms. Moreover, chronic biofilm-related infections can lead to the occurrence of systemic diseases, as in the case of chronic periodontitis, linked to atherosclerosis, cardiovascular disease and diabetes. The more researchers discover, new unknown issues add up to the complexity of biofilm infections, in which microbial species establish relationships of cooperation and competition, and elaborate phenotypic differentiation into functional, adapted communities. Their interaction with the host's immune system or with therapeutic agents contributes to the complex puzzle that still misses a lot of pieces. In this comprehensive review we aimed to highlight the microbial composition, developmental stages, architecture and properties of medical biofilms, as well as the diagnostic tools used in the management of biofilm related infections. Also, we present recently acquired knowledge on the etiopathogenesis, diagnosis and treatment of four chronic diseases associated with biofilm development in tissues (chronic periodontitis, chronic lung infection in cystic fibrosis, chronic wounds) and artificial substrata (medical devices-related infections).
    Current topics in medicinal chemistry 04/2015; 15(999). DOI:10.2174/1568026615666150414123800 · 3.40 Impact Factor
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    ABSTRACT: At present, the use of dental implants is a very common practice as tooth loss is a frequent problem and can occur as a result of disease or trauma. An implant is usually made of biocompatible materials that do not cause rejection reactions and allow the implant union with the respective bone. To achieve this goal, the implant surface may have different structures and coatings, generally used to increase the adherence of the implant to the bone and to decrease the risk of the periimplantar inflammatory reactions. This review gives some insights of the metal based materials used for dental implants, their limits, improvement strategies as well as the pathophysiology, diagnosis, treatment and prevention of periimplantary diseases.
    Current topics in medicinal chemistry 04/2015; 15(999). DOI:10.2174/1568026615666150414144033 · 3.40 Impact Factor
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    ABSTRACT: The spectacular progress of research in the nanotechnology field led to the achievement of important knowledge of materials at the atomic and molecular scale and the extent of the use of nanoparticles in the design of medical products, ecological processes, cosmetics and other biotechnological processes. Nanotechnology has become increasingly employed for medical applications and is particularly of great interest especially for developing new strategies to inhibit the activity of different microorganisms. The purpose of this review was to present the antimicrobial activity of metal cations in micro- and nanoparticulate forms and the dependence of this biological activity on shape, size and physico-chemical conditions.
    Current topics in medicinal chemistry 04/2015; 15(999). DOI:10.2174/1568026615666150414125015 · 3.40 Impact Factor

Publication Stats

784 Citations
203.51 Total Impact Points


  • 2008-2015
    • University of Bucharest
      • • Faculty of Biology
      • • Department of Botany and Microbiology
      Bucureşti, Bucureşti, Romania
    • Institutul National de Cercetari Economice (INCE)
      Bucureşti, Hunedoara, Romania
  • 2009-2012
    • Institutul Clinic Fundeni
      Bucureşti, Bucureşti, Romania
  • 2007-2011
    • Cantacuzino Institute
      Bucureşti, Bucureşti, Romania
  • 2010
    • Carol Davila University of Medicine and Pharmacy
      • Faculty of Pharmacy
      Bucureşti, Bucureşti, Romania