Pei-Jin Zhou

Jiangsu University, Zhenjiang, Jiangsu Sheng, China

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Publications (40)83.55 Total impact

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    ABSTRACT: Two halophilic archaea, strains TBN53(T) and CSW2.24.4(T), were characterized to elucidate their taxonomic status. Strain TBN53(T) was isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China, whereas strain CSW2.24.4(T) was isolated from a saltern crystallizer in Victoria, Australia. Cells of the two strains were pleomorphic, stained Gram-negative and produced red-pigmented colonies. Strain TBN53(T) was able to grow at 25-55 °C (optimum 45 °C), with 1.4-5.1 M NaCl (optimum 2.6-3.9 M NaCl), with 0-1.0 M MgCl(2) (optimum 0-0.1 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0), whereas strain CSW2.24.4(T) was able to grow at 25-45 °C (optimum 37 °C), with 2.6-5.1 M NaCl (optimum 3.4 M NaCl), with 0.01-0.7 M MgCl(2) (optimum 0.05 M MgCl(2)) and at pH 5.5-9.5 (optimum pH 7.0-7.5). Cells of the two isolates lysed in distilled water. The minimum NaCl concentrations that prevented cell lysis were 8 % (w/v) for strain TBN53(T) and 12 % (w/v) for strain CSW2.24.4(T). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate, with two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Trace amounts of other unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strains TBN53(T) and CSW2.24.4(T) showed 94.1 % similarity to each other and were closely related to Halobellus clavatus TNN18(T) (95.0 and 94.7 % similarity, respectively). Levels of rpoB' gene sequence similarity between strains TBN53(T) and CSW2.24.4(T), and between these strains and Halobellus clavatus TNN18(T) were 88.5, 88.5 and 88.1 %, respectively. The DNA G+C contents of strains TBN53(T) and CSW2.24.4(T) were 69.2 and 67.0 mol%, respectively. The level of DNA-DNA relatedness between strain TBN53(T) and strain CSW2.24.4(T) was 25 %, and these two strains showed low levels of DNA-DNA relatedness with Halobellus clavatus TNN18(T) (30 and 29 % relatedness, respectively). Based on these phenotypic, chemotaxonomic and phylogenetic properties, two novel species of the genus Halobellus are proposed to accommodate these two strains, Halobellus limi sp. nov. (type strain TBN53(T) = CGMCC 1.10331(T) = JCM 16811(T)) and Halobellus salinus sp. nov. (type strain CSW2.24.4(T) = DSM 18730(T) = CGMCC 1.10710(T) = JCM 14359(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; 62(Pt 6):1307-13. · 2.11 Impact Factor
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    ABSTRACT: Two extremely halophilic archaeal strains GX3(T) and GX26(T) were isolated from the Gangxi marine solar saltern near the Weihai city of Shandong Province, China. Cells from the two strains were pleomorphic and stained Gram-negative, colonies were red-pigmented. Strains GX3(T) and GX26(T) were able to grow at 25-50 °C (optimum 37 °C), at 1.4-5.1M NaCl (optimum 3.1M), at pH 5.5-9.5 (optimum pH 7.0) and neither strain required Mg(2+) for growth. Cells lyse in distilled water and the minimal NaCl concentration to prevent cell-lysis was 8% (w/v). The major polar lipids of the two strains were PA (phosphatidic acid), PG (phosphatidylglycerol), PGP-Me (phosphatidylglycerol phosphate methyl ester) and three major glycolipids (GL1, GL2 & GL3) chromatographically identical to S-TGD-1 (sulfated galactosyl mannosy glucosyl diether), S-DGD-1 (sulfated mannosyl glucosyl diether), and DGD-1 (mannosyl glucosyl diether) respectively, an unidentified lipid (GL4) was also detected in strain GX26(T). Phylogenetic analysis based on 16S rRNA gene revealed that strain GX3(T) and strain GX26(T) formed a distinct clade with the closest relative, Haladaptatus paucihalophilus (89.9-92.4% and 90.4-92.7, respectively). The rpoB' gene similarities between strains GX3(T) and GX26(T), and between the two strains and the closest relative, Halorussus rarus TBN4(T) are 96.5%, 84.3% and 83.9%, respectively. The DNA G+C contents of strain GX3(T) and strain GX26(T) are 67.3 mol% and 67.2 mol%, respectively. The DNA-DNA hybridization value between strain GX3(T) and strain GX26(T) was 44%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain GX3(T) and strain GX26(T) represent two novel species in a new genus within the family Halobacteriaceae, Halorubellus salinus gen. nov., sp. nov. (type strain GX3(T)=CGMCC 1.10384(T)=JCM 17115(T)) and Halorubellus litoreus sp. nov. (type strain GX26(T)=CGMCC 1.10386(T)=JCM 17117(T)).
    Systematic and Applied Microbiology 09/2011; 35(1):30-4. · 3.29 Impact Factor
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    ABSTRACT: Two halophilic archaea, strains RO1-4(T) and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4(T) and RO1-64 were able to grow at 25-55 °C (optimum 40-42 °C), at 2.1-5.1 M NaCl (optimum 3.9 M NaCl), at 0.05-0.7 M MgCl(2) (optimum 0.3 M MgCl(2)) and at pH 6.0-8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4(T) and RO1-64 were closely related to Halogeometricum borinquense PR3(T) (98.0 and 98.2 % similarity, respectively) and Halosarcina pallida BZ256(T) (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4(T) and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA-DNA hybridization value between strains RO1-4(T) and RO1-64 was 83.0 % and both strains showed low DNA-DNA relatedness with Halogeometricum borinquense PR3(T) (42.5 and 50.1 % relatedness, respectively) and Halosarcina pallida BZ256(T) (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4(T) and RO1-64 represent a novel species of the genus Halogeometricum, for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4(T) (=CGMCC 1.7736(T) =JCM 15770(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2009; 60(Pt 11):2613-7. · 2.11 Impact Factor
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    ABSTRACT: A halophilic archaeon, strain RO1-6(T), was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6(T) were pleomorphic and motile and stained Gram-negative. Strain RO1-6(T) grew well on complex medium and colonies were red-pigmented. It was able to grow at 20-50°C (optimum 37°C), in 2.1-5.1M NaCl (optimum 3.9 M NaCl), in 0.05-0.70 M MgCl₂ (optimum 0.30 M MgCl₂) and at pH 6.5-8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S₂-DGD. The 16S rRNA gene sequence of strain RO1-6(T) showed similarities of 96.9 and 96.4 % to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6(T) was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6(T) is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6(T) (=CGMCC 1.8711(T) =JCM 16054(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2009; 60(Pt 10):2462-3466. · 2.11 Impact Factor
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    ABSTRACT: Two extremely halophilic archaea, strains RO5-2(T) and RO5-14, were isolated from Rudong marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile and stained Gram-negative. Colonies were red-pigmented. Strains RO5-2(T) and RO5-14 were able to grow at 20-50 degrees C (optimum 37 degrees C), at 2.6-4.8 M NaCl (optimum 3.4-3.9 M NaCl), at 0.03-0.7 M MgCl(2) (optimum 0.5 M MgCl(2)) and at pH 5.5-8.0 (optimum pH 6.5-7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene sequences of strains RO5-2(T) and RO5-14 showed 93.4-93.8 % similarity to the closest cultivated relative, Halosarcina pallida. The DNA G+C content of strains RO5-2(T) and RO5-14 was 61.0 mol% and 59.9 mol%, respectively. The DNA-DNA relatedness between strains RO5-2(T) and RO5-14 was 86.0 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains RO5-2(T) and RO5-14 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halopelagius inordinatus gen. nov., sp. nov. is proposed. The type strain is RO5-2(T) (=CGMCC 1.7739(T) =JCM 15773(T)).
    International journal of systematic and evolutionary microbiology 10/2009; 60(Pt 9):2089-93. · 2.11 Impact Factor
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    ABSTRACT: An extremely halophilic archaeon, strain RO5-8T, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8T stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30-50 degrees C (optimum 40 degrees C), at 2.6-4.3 M NaCl (optimum 3.1 M NaCl), at 0.03-0.5 M MgCl2 (optimum 0.03 M MgCl2) and at pH 5.5-7.5 (optimum pH 6.0-6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12% (w/v). The major polar lipids of strain RO5-8T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8T was closely related to three strains of Haloplanus natans with similarities of 97.3-97.6%. The DNA G+C content of strain RO5-8T was 62.1 mol%. The DNA-DNA hybridization value between strain RO5-8T and Haloplanus natans JCM 14081T was 51.6%. It was concluded that strain RO5-8T represents a novel species of the genus Haloplanus, for which the name Haloplanus vescus sp. nov. is proposed. The type strain is RO5-8T (=CGMCC 1.8712T =JCM 16055T).
    International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 8):1824-7. · 2.11 Impact Factor
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    ABSTRACT: Two extremely halophilic archaea, strains RO1-28(T) and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg(2+) and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0-8.5 and a temperature range of 25-55 degrees C, with optimal pH of 7.0 and optimal temperature of 37-40 degrees C. Based on 16S rRNA gene sequence analysis, strains RO1-28(T) and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0-95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897(T). Both strains RO1-28(T) and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA-DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA-DNA relatedness (32 % and 33 %) with Haladaptatus paucihalophilus JCM 13897(T). It was concluded that strains RO1-28(T) and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28(T) (=CGMCC 1.7737(T) =JCM 15771(T)).
    International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 5):1085-9. · 2.11 Impact Factor
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    ABSTRACT: Two extremely halophilic archaea, strains RO2-11(T) and HO2-1, were isolated from two Chinese marine solar salterns, Rudong solar saltern and Haimen solar saltern, respectively. Cells of the two strains were polymorphic and Gram-stain-negative; colonies were red-pigmented. The two strains grew at NaCl concentrations of 2.6-4.3 M (optimum 3.9 M) and required at least 0.1 M Mg2+ for growth. They were able to grow over a pH range of 6.0-8.0 and a temperature range of 20-50 degrees C, with optimal pH of 7.5 and optimal temperature of 37 degrees C. The major polar lipids of strain RO2-11(T) and strain HO2-1 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three glycolipids, two of them chromatographically identical to S-DGD-1 and DGD-1, the third unidentified. The 16S rRNA gene sequence similarity of strain RO2-11(T) and strain HO2-1 was 99.3 % and highest sequence similarity with the closest relative (Haloferax larsenii) was 91.4 %. Based on the data obtained, the two isolates could not be classified in any recognized genus of the family Halobacteriaceae. Strain RO2-11(T) and strain HO2-1 are thus considered to represent a novel species of a new genus within the family Halobacteriaceae, for which the name Halogranum rubrum gen. nov., sp. nov. is proposed. The type strain is RO2-11(T) (=CGMCC 1.7738(T) =JCM 15772(T)).
    International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 6):1366-71. · 2.11 Impact Factor
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    ABSTRACT: A novel cold-living, Gram-stain-negative, yellow-pigmented and obligately aerobic bacterium designated strain 0563(T) was isolated from the China No. 1 glacier. Phylogenetic analysis showed that strain 0563(T) was a member of the genus Flavobacterium, sharing the highest 16S rRNA gene sequence similarities with Flavobacterium limicola JCM 11473(T) (98.5 %) and Flavobacterium psychrolimnae NBRC 102679(T) (98.2 %). The genomic DNA G+C content was 34.8 mol%. Strain 0563(T) displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH, summed feature 3 (comprising C(16 : 1)omega7c and/or C(16 : 1)omega6c), C(15 : 1)omega6c, iso-C(15 : 1) G and iso-C(16 : 0) 3-OH as the major fatty acids. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, the novel species Flavobacterium tiangeerense sp. nov. is proposed; the type strain is 0563(T) (=CGMCC 1.6847(T) =JCM 15087(T)).
    International journal of systematic and evolutionary microbiology 08/2009; 59(Pt 11):2773-7. · 2.11 Impact Factor
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    ABSTRACT: A novel aerobic, psychrotolerant, yellow-to-orange bacterium (strain 0423(T)) was isolated from the China No. 1 glacier. Strain 0423(T) displayed phenotypic and chemotaxonomic features of the genus Planomicrobium, containing anteiso-C(15 : 0) and iso-C(14 : 0) as the major fatty acids. The temperature range for growth was 4-28 degrees C, with optimum growth at 20-21 degrees C. The genomic DNA G+C content was 49 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0423(T) was related to members of the genus Planomicrobium, sharing the highest sequence similarities with the type strains of Planomicrobium chinense, P. mcmeekinii, P. okeanokoites and P. koreense. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species, Planomicrobium glaciei sp. nov., is proposed. The type strain is 0423(T) (=CGMCC 1.6846(T) =JCM 15088(T)).
    International journal of systematic and evolutionary microbiology 07/2009; 59(Pt 6):1387-90. · 2.11 Impact Factor
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    ABSTRACT: A novel psychrophilic, Gram-positive, yellow-pigmented and aerobic bacterium, strain 0543(T), was isolated from the China No. 1 glacier. Strain 0543(T) was able to grow at 4-23 degrees C, with optimum growth at 18-19 degrees C. The major fatty acids were anteiso-C(15 : 0) (58.36 %), iso-C(16 : 0) (21.13 %), iso-C(14 : 0) (10.25 %) and anteiso-C(17 : 0) (7.16 %). The genomic DNA G+C content was 63.5 mol% and the major menaquinone was MK-10. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 0543(T) represented a lineage within the family Microbacteriaceae, with the highest similarity of 97.4 % with Salinibacterium amurskyense KMM 3673(T). On the basis of polyphasic, chemotaxonomic, physiological and biochemical evidence from this study, the novel species Salinibacterium xinjiangense sp. nov. is proposed; the type strain is 0543(T)(=CGMCC 1.5381(T) =JCM 13926(T)).
    International journal of systematic and evolutionary microbiology 01/2009; 58(Pt 12):2739-42. · 2.11 Impact Factor
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    ABSTRACT: All members of the genera Haloarcula and Halomicrobium whose names have been validly published were surveyed for 16S rRNA gene polymorphism, and the transcription of the genes from two species was investigated during growth at different NaCl concentrations. The species of Haloarcula and Halomicrobium harbour at least two different 16S rRNA gene copies, and 18 new sequences of 16S rRNA genes were obtained. The type I and type II 16S rRNA genes of Haloarcula are divergent at 4.8-5.6% of their nucleotide positions. The type III and type IV 16S rRNA genes from Halomicrobium mukohataei JCM 9738(T) are 9.0% divergent, which represents the highest intraspecific divergent 16S rRNA genes so far seen. Phylogenetic analysis based on 16S rRNA genes indicated that all type I 16S rRNA genes were clustered, and the same was true for the type II 16S rRNA genes of Haloarcula species. The two clusters, respectively generated from type I and type II 16S rRNA genes, were sharply separated and their divergences (4.8-5.6%) are in the range of various divergence usually found between genera in the order Halobacteriales (about 5-10%). Results from reverse transcription-PCR showed that the type I and type II copies of Har. amylolytica BD-3(T) and type III and type IV copies of Hmc. mukohataei JCM 9738(T) were all transcribed to 16S rRNA molecules under different salt concentrations (15-28% NaCl).
    Extremophiles 11/2008; 13(1):31-7. · 2.20 Impact Factor
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    ABSTRACT: A Gram-positive, psychrophilic, rod-shaped bacterium, designated strain N-05(T), was isolated from soil samples collected off King George Island, west Antarctica (6 degrees 13' 31'' S 5 degrees 57' 08'' W). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N-05(T) was related to members of the genus Sporosarcina and had highest 16S rRNA gene sequence similarity with the type strain of Sporosarcina macmurdoensis (98.0%). The temperature range for growth of strain N-05(T) was 0-23 degrees C, with optimum growth occurring at 17-18 degrees C and approximately pH 6.0-8.0. Strain N-05(T) had MK-7 as the major menaquinone and anteiso-C(15:0) and C(16:1)omega7c alcohol as major fatty acids. The genomic DNA G+C content was 39.2 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain N-05(T) is considered to represent a novel species of the genus Sporosarcina, for which the name Sporosarcina antarctica is proposed. The type strain is N-05(T) (=CGMCC 1.6503(T)=JCM 14646(T)).
    International journal of systematic and evolutionary microbiology 10/2008; 58(Pt 9):2114-7. · 2.11 Impact Factor
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    ABSTRACT: A novel psychrotolerant, Gram-negative, aerobic bacterium, designated strain 537T, was isolated from sea-ice samples from the Arctic. Strain 537T was able to grow at 4-26 degrees C, with optimum growth occurring at 20-21 degrees C. Strain 537T had Q-8 as the major respiratory quinone and contained iso-C15:0 2-OH and/or C16:1 omega7c (22.95 %), C15:1 (17.64 %) and C17:1 omega8c (13.74 %) as the predominant cellular fatty acids. The genomic DNA G+C content was 38.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 537T formed a coherent cluster within the genus Colwellia. The highest level of 16S rRNA gene sequence similarity (97.5 %) exhibited by strain 537T was obtained with respect to the type strain of Colwellia aestuarii. On the basis of phenotypic, chemotaxonomic and phylogenetic properties and DNA-DNA relatedness data, strain 537T represents a novel species of the genus Colwellia, for which the name Colwellia polaris sp. nov. is proposed. The type strain is 537T (=CGMCC 1.6132T =JCM 13952T).
    International journal of systematic and evolutionary microbiology 09/2008; 58(Pt 8):1931-4. · 2.11 Impact Factor
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    ABSTRACT: A novel psychrotolerant, Gram-negative, motile bacterium, designated strain 328(T), was isolated from sea-ice samples collected off the Canadian Basin of the Arctic Ocean (7 degrees 23' 14'' N 14 degrees 06' 55'' W). Strain 328(T) was able to grow at 0-37 degrees C, with optimum growth at 25-27 degrees C. It possessed phosphatidylethanolamine and phosphatidylglycerol as major phospholipids and C(10 : 0) 3-OH (31.78 %), C(18 : 1)omega7c (27.50 %) and iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (19.22 %) as predominant cellular fatty acids. The DNA G+C content of strain 328(T) was 45.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 328(T) was a member of the genus Marinomonas (92.7-96.0 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 328(T) was considered to represent a novel species of the genus Marinomonas. The name Marinomonas arctica sp. nov. is proposed, with strain 328(T) (=CGMCC 1.6498(T)=JCM 14976(T)) as the type strain.
    International journal of systematic and evolutionary microbiology 08/2008; 58(Pt 7):1715-8. · 2.11 Impact Factor
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    ABSTRACT: Strain 20041(T) was isolated from sea-ice of the Canadian Basin (7 degrees 23' 14'' N 14 degrees 06' 55'' W). Phylogenetic analysis based on 16S rRNA gene homology showed that strain 20041(T) was related to members of the genus Marinobacter and had highest 16S rRNA gene sequence similarity with the type strain of Marinobacter maritimus. Cells were Gram-negative, rod-shaped, psychrophilic and motile. The temperature range for growth was 0-22 degrees C, with optimum growth occurring at 16-18 degrees C and at approximately pH 6.0-9.0. Strain 20041(T) had ubiquinone-9 as the major respiratory quinone and iso-C(15 : 0) 2OH and/or C(16 : 1)omega7c, C(16 : 0), C(18 : 1)omega9c and C(12 : 0) 3OH as major fatty acids. The genomic DNA G+C content was 55.4 mol%. On the basis of the phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain 20041(T) is considered to represent a novel species, for which the name Marinobacter psychrophilus sp. nov. is proposed. The type strain is 20041(T) (=CGMCC 1.6499(T)=JCM 14643(T)).
    International journal of systematic and evolutionary microbiology 06/2008; 58(Pt 6):1463-6. · 2.11 Impact Factor
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    ABSTRACT: A Gram-negative, psychrophilic, motile, rod-shaped bacterium, designated strain 20188(T), was isolated from marine samples collected from the Arctic (7 degrees 00' 24'' N 16 degrees 59' 37'' W), and was identified taxonomically by means of a polyphasic study. On the basis of 16S rRNA gene sequence similarity, strain 20188(T) was closely related to members of the genera Phaeobacter. 16S rRNA gene sequence similarities between strain 20188(T) and the type strains of Phaeobacter inhibens, Phaeobacter gallaeciensis and Phaeobacter daeponensis were 97.0, 96.8 and 96.2 %, respectively. The temperature range for growth was 0-25 degrees C, with optimum growth occurring at 19-20 degrees C and at approximately pH 6.0-9.0. Strain 20188(T) had ubiquinone-10 as the major respiratory quinone and C(18 : 1)omega7c and 11-methyl C(18 : 1)omega7c as major fatty acids. The genomic DNA G+C content was 59.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain 20188(T) is considered to represent a novel species of the genus Phaeobacter, for which the name Phaeobacter arcticus sp. nov. is proposed. The type strain is 20188(T) (=CGMCC 1.6500(T)=JCM 14644(T)).
    International journal of systematic and evolutionary microbiology 06/2008; 58(Pt 6):1384-7. · 2.11 Impact Factor
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    ABSTRACT: An extremely halophilic archaeon, strain Fa-1(T), was isolated from a marine solar saltern in Fujian, China. Strain Fa-1(T) required Mg2+ and at least 2.0 M NaCl for growth. It was able to grow at pH 6.5-9.0 (optimally at pH 7.0-7.5) and at 20-55 degrees C (optimally at 37-42 degrees C). The major polar lipids of strain Fa-1(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a sulfated diglycosyl diether. On the basis of a 16S rRNA gene sequence analysis, strain Fa-1(T) was closely related to nine species of the genus Halorubrum, showing sequence similarities of 97.4-98.4 %. The G+C content of the DNA of strain Fa-1(T) is 64.9 mol% (Tm). DNA-DNA hybridization values between strain Fa-1(T) and the most closely related members of the genus Halorubrum were below 51 %. On the basis of the data from this study, strain Fa-1(T) represents a novel species of the genus Halorubrum, for which the name Halorubrum litoreum sp. nov. is proposed. The type strain is Fa-1(T) (=CGMCC 1.5336(T) =JCM 13561(T)).
    International journal of systematic and evolutionary microbiology 11/2007; 57(Pt 10):2204-6. · 2.11 Impact Factor
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    ABSTRACT: An extremely haloalkaliphilic archaeon, strain AD2(T), was isolated from Aiding salt lake in Xin-Jiang, China. Strain AD2(T) required at least 12 % NaCl for growth. MgCl(2) was not required. The isolate was able to grow over a pH range of 8.0-10.0 and temperature range of 20-55 degrees C, with optimal growth at pH 8.7-9.2 and 44-47 degrees C. The major polar lipids of strain AD2(T) were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; glycolipids were not detected. Analysis of its 16S rRNA gene sequence indicated that strain AD2(T) was phylogenetically related to members of the genus Natronorubrum, with sequence similarities to the type strains of Natronorubrum bangense, Natronorubrum tibetense and Natronorubrum aibiense of 97.1, 95.9 and 96.1 %, respectively. The G+C content of its DNA was 60.9 mol% (T(m)). Levels of DNA-DNA relatedness between strain AD2(T) and the type strains of Nrr. bangense, Nrr. tibetense and Nrr. aibiense were 49, 38 and 41 %, respectively. It was concluded that strain AD2(T) represents a novel species of the genus Natronorubrum, for which the name Natronorubrum sulfidifaciens sp. nov. is proposed. The type strain is AD2(T) (=CGMCC 1.6307(T)=JCM 14089(T)).
    International journal of systematic and evolutionary microbiology 04/2007; 57(Pt 4):738-40. · 2.11 Impact Factor
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    ABSTRACT: A novel psychrotolerant, Gram-positive, yellow-pigmented, aerobic bacterium, strain 0549(T), was isolated from the China No. 1 glacier. The cells of the isolate were catalase-positive, motile, irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain 0549(T) was able to grow at 4-27 degrees C, with optimum growth occurring at 20-22 degrees C. The major fatty acids were anteiso-C(15 : 0), anteiso-C(15 : 1), iso-C(16 : 0) and anteiso-C(17 : 0). The genomic DNA G+C content was 67 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 0549(T) belonged to the genus Cryobacterium and exhibited 96.5 % similarity to Cryobacterium psychrophilum JCM 1463(T). On the basis of the phenotypic, chemotaxonomic and phylogenetic properties and the DNA-DNA relatedness data, strain 0549(T) represents a novel species of the genus Cryobacterium, for which the name Cryobacterium psychrotolerans is proposed. The type strain is 0549(T) (=CGMCC 1.5382(T)=JCM 13925(T)).
    International journal of systematic and evolutionary microbiology 04/2007; 57(Pt 4):866-9. · 2.11 Impact Factor

Publication Stats

511 Citations
83.55 Total Impact Points

Institutions

  • 2007–2011
    • Jiangsu University
      • School of Food and Biological Engineering
      Zhenjiang, Jiangsu Sheng, China
    • China Agricultural University
      • State Key Laboratory for Agrobiotechnology
      Beijing, Beijing Shi, China
    • Xinjiang Normal University
      Hsin-chien, Jiangxi Sheng, China
  • 2004–2011
    • Chinese Academy of Sciences
      • • State Key Laboratory of Microbial Resources
      • • Institute of Microbiology
      Peping, Beijing, China
  • 2003–2009
    • Northeast Institute of Geography and Agroecology
      • • Institute of Microbiology
      • • State Key Laboratory of Microbial Resources
      Beijing, Beijing Shi, China
  • 2008
    • Ocean University of China
      Tsingtao, Shandong Sheng, China
    • Polar Research Institute of China
      Shanghai, Shanghai Shi, China
  • 2005
    • IT University of Copenhagen
      København, Capital Region, Denmark
    • Zhejiang University
      • College of Life Sciences
      Hangzhou, Zhejiang Sheng, China