Duangkhae Srikun

Publications of Duangkhae Srikun

• A two-photon fluorescent probe for ratiometric imaging of hydrogen peroxide in live tissue.

  Authors: Chul Chung, Duangkhae Srikun, Chang Su Lim, Christopher J Chang, Bong Rae Cho

  Chemical communications (Cambridge, England). 09/2011; 47(34):9618-20.

  We report a two-photon fluorescent probe (PN1) that can be excited by 750 nm femto-second pulses, shows high photostability and negligible toxicity, and can visualize H(2)O(2) distribution in liveWe report a two-photon fluorescent probe (PN1) that can be excited by 750 nm femto-second pulses, shows high photostability and negligible toxicity, and can visualize H(2)O(2) distribution in live cells and tissue by two-photon microscopy.

• Organelle-targetable fluorescent probes for imaging hydrogen peroxide in living cells via SNAP-Tag protein labeling.

  Authors: Duangkhae Srikun, Aaron E Albers, Christine I Nam, Anthony T Iavarone, Christopher J Chang

  Journal of the American Chemical Society. 03/2010; 132(12):4455-65.

  Hydrogen peroxide (H(2)O(2)) is a potent small-molecule oxidant that can exert a diverse array of physiological and/or pathological effects within living systems depending on the timing and locationHydrogen peroxide (H(2)O(2)) is a potent small-molecule oxidant that can exert a diverse array of physiological and/or pathological effects within living systems depending on the timing and location of its production, accumulation, trafficking, and consumption. To help study the chemistry and biology of this reactive oxygen species (ROS) in its native cellular context, we now present a new method for monitoring local, subcellular changes in H(2)O(2) levels by fluorescence imaging. Specifically, we have exploited the versatility of the SNAP-tag technology for site-specific protein labeling with small molecules on the surface or interior of living cells with the use of boronate-capped dyes to selectively visualize H(2)O(2). The resulting SNAP-Peroxy-Green (SNAP-PG) probes consist of appropriately derivatized boronates bioconjugated to SNAP-tag fusion proteins. Spectroscopic measurements of the SNAP-PG constructs confirm their ability to detect H(2)O(2) with specificity over other biologically relevant ROS. Moreover, these hybrid small-molecule/protein reporters can be used in live mammalian cells expressing SNAP-tag fusion proteins directed to the plasma membrane, nucleus, mitochondria, and endoplasmic reticulum. Imaging experiments using scanning confocal microscopy establish organelle-specific localization of the SNAP-tag probes and their fluorescence turn-on in response to changes in local H(2)O(2) levels. This work provides a general molecular imaging platform for assaying H(2)O(2) chemistry in living cells with subcellular resolution.

• Mitochondrial-targeted fluorescent probes for reactive oxygen species.

  Authors: Bryan C Dickinson, Duangkhae Srikun, Christopher J Chang

  Current opinion in chemical biology. 11/2009;

  As the primary consumers of oxygen within all aerobic organisms, mitochondria are a major source of cellular reactive oxygen species (ROS) derived from the in vivo chemistry of oxygen metabolism.As the primary consumers of oxygen within all aerobic organisms, mitochondria are a major source of cellular reactive oxygen species (ROS) derived from the in vivo chemistry of oxygen metabolism. Mitochondrial ROS have been traditionally implicated in aging and in a variety of pathologies, including cancer, neurodegeneration, and diabetes, but recent studies also link controlled mitochondrial ROS fluxes to cell regulation and signaling events. Progress in the development of mitochondrial-targeted fluorescent small-molecule indicators that detect specific ROS with high selectivity offers a promising approach for interrogating mitochondrial ROS production, trafficking, and downstream biological effects.

• An ICT-based approach to ratiometric fluorescence imaging of hydrogen peroxide produced in living cells.

  Authors: Duangkhae Srikun, Evan W Miller, Dylan W Domaille, Christopher J Chang

  Journal of the American Chemical Society. 05/2008; 130(14):4596-7.