Evelina L Zdorovenko

Publications (28)61.72 Total impact

• Article: Immunochemical Characterization of the Capsular Polysaccharide of Azospirillum irakense KBC1.

  Yulia P Fedonenko, Gennady L Burygin, Irina A Popova, Elena N Sigida, Alina K Surkina, Evelina L Zdorovenko, Svetlana A Konnova
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  ABSTRACT: The repeating unit structure of Azospirillum irakense KBC1 capsular polysaccharide (CPS) was established and was found to be identical to that of the O polysaccharide of A. irakense KBC1 lipopolysaccharide (LPS). The antigenic heterogeneity of the LPS and the CPS was shown to be related to differences in the macromolecular organization of these glycopolymers. After an immune response activation, R-form CPS molecules were found to be predominant.
  Current Microbiology 03/2013; · 1.82 Impact Factor
• Article: Structures of cell-wall phosphate-containing glycopolymers of Bifidobacterium longum BIM B-476-D.

  Olga A Valueva, Alexander S Shashkov, Evelina L Zdorovenko, Alexander O Chizhov, Elena Kiseleva, Galina Novik, Yuriy A Knirel
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  ABSTRACT: Glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of Bifidobacterium longum BIM B-476-D by stepwise extraction with 10% CCl3CO2H. The following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% HOAc, dephosphorylation with 48% HF, 2D NMR spectroscopy, and high-resolution ESI MS: The ribitol teichoic acid also contains minor d-alanine, whose position was not determined.
  Carbohydrate research 03/2013; 373C:22-27. · 2.03 Impact Factor
• Article: Structure of repeating units of a polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense SR80.

  Elena N Sigida, Yuliya P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Alexander S Shashkov, Vladimir V Ignatov, Yuriy A Knirel
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  ABSTRACT: A high-molecular mass polysaccharide fraction was obtained by mild acid hydrolysis of the lipopolysaccharide of diazotrophic rhizobacterium Azospirillum brasilense SR80 followed by GPC on Sephadex G-50 Superfine. Studies by composition and methylation analyses, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy demonstrated the presence of two structurally distinct repeating units having the following structures:It seems likely, although not proved, that these are repeats of two distinct polysaccharides rather than they build blocks within the same polysaccharide chain. The former structure is new, whereas the latter is closely related to the O-polysaccharide structure of A. brasilense Jm6B2 established earlier, which differs in partial (∼60%) 3-O-methylation of d-rhamnose only [Boiko, A. S.; Dmitrenok, A. S.; Fedonenko, Yu. P.; Zdorovenko, E. L.; Konnova, S. A.; Knirel, Y. A.; Ignatov, V. V. Carbohydr. Res.2012, 355, 92-95].
  Carbohydrate research 01/2013; 371C:40-44. · 2.03 Impact Factor
• Article: Structure of the O-polysaccharide of Azorhizobium caulinodans HAMBI 216; identification of 3-C-methyl-D-rhamnose as a component of bacterial polysaccharides.

  Evelina L Zdorovenko, Olga A Valueva, Vadim V Kachala, Alexander S Shashkov, Yuriy A Knirel, Iwona Komaniecka, Adam Choma
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  ABSTRACT: The O-polysaccharide was obtained from the lipopolysaccharide of the stem-nodulating nitrogen-fixing bacterium Azorhizobium caulinodans HAMBI 216 and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy. The polysaccharide was found to have a linear pentasaccharide repeating unit containing D-rhamnose and its rarely occurring 2-O-methyl (Rha2OMe) and 3-C-methyl (Rha3CMe) derivatives and having the following structure: →3)-α-D-Rhap2OMe-(1→2)-β-D-Rhap3CMe-(1→3)-α-D-Rhap-(1→2)-β-D-Rhap3CMe-(1→3)-α-D-Rhap-(1→ .
  Carbohydrate research 06/2012; 358:106-9. · 2.03 Impact Factor
• Article: Structural studies of the O-polysaccharide of Pragia fontium 97U124 containing 2-acetamido-2,4,6-trideoxy-4-(D-glyceroyl)amino-D-glucose.

  Olga A Valueva, Evelina L Zdorovenko, Lyudmyla D Varbanets, Vladimir V Shubchinskiy, Alexander S Shashkov, Yuriy A Knirel
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  ABSTRACT: The O-polysaccharide was obtained by acid hydrolysis of the lipopolysaccharide of Pragia fontium 97U124 and studied by sugar analysis and 1D and 2D NMR spectroscopy. A new bacillosamine derivative, 2-acetamido-2,4,6-trideoxy-4-(D-glyceroyl)amino-D-glucose (D-QuiNAc4NAcyl), was identified as a polysaccharide constituent. The following structure of the O-polysaccharide was established →3)-α-L-FucpNAc-(1→3)-α-L-FucpNAc-(1→3)-β-D-QuipNAc4NAcyl-(1→ This structure is closely related to that of the D-QuiNAc4NAc-containing O-polysaccharide of Pseudomonas aurantiaca IMV 31 established earlier (Knirel, Y. A.; Zdorovenko, G. M.; Veremeychenko, S. N.; Lipkind, G. M.; Shashkov, A. S.; Zakharova, I. Y.; Kochetkov, N. K. Bioorg. Khim.1988, 14, 352-358).
  Carbohydrate research 04/2012; 355:96-9. · 2.03 Impact Factor
• Article: Structural analysis of the O-polysaccharide of the lipopolysaccharide from Azospirillum brasilense Jm6B2 containing 3-O-methyl-D-rhamnose (D-acofriose).

  Alevtina S Boyko, Andrey S Dmitrenok, Yuliya P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Yuriy A Knirel, Vladimir V Ignatov
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  ABSTRACT: Two types of neutral O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide isolated by phenol-water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense Jm6B2. The following structure of the major O-polysaccharide was established by composition and methylation (ethylation) analyses, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy: [structure: see text] where a non-stoichiometric (~60%) 3-O-methylation of D-rhamnose is indicated by italics.
  Carbohydrate research 04/2012; 355:92-5. · 2.03 Impact Factor
• Article: Structure of the O-antigen of Budvicia aquatica 20186, a new bacterial polysaccharide that contains 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-D-xylo-hexose (yersiniose A).

  Evelina L Zdorovenko, Olga A Valueva, Liudmyla D Varbanets, Alexander S Shashkov, Yuriy A Knirel
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  ABSTRACT: The following structure of the O-specific polysaccharide (O-antigen) of Budvicia aquatica 20186 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-α-L-Rhap-(1→3)-α-D-Galp-(1→2)-α-Yerp-(1→3)-β-D-GalpNAc-(1→ where Yer stands for 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-D-xylo-hexose (yersiniose A).
  Carbohydrate research 02/2012; 352:219-22. · 2.03 Impact Factor
• Article: An improved rapid method for the preparation of D-rhamnose.

  Olga N Smol'kina, Natalya S Shishonkova, Yulia P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Vladimir V Ignatov
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  ABSTRACT: A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was crystallized. The sugar activity was demonstrated immunochemically. The advantages of the method are that it expedites and simplifies the extraction of D-rhamnose and increases its yield.
  Carbohydrate research 10/2011; 347(1):161-3. · 2.03 Impact Factor
• Article: Structure of the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582: identification of 4-deoxy-D-xylo-hexose as a component of bacterial polysaccharides.

  Olga A Valueva, Dzianis Rakhuba, Alexander S Shashkov, Evelina L Zdorovenko, Elena Kiseleva, Galina Novik, Yuriy A Knirel
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  ABSTRACT: A novel constituent of bacterial polysaccharides, 4-deoxy-D-xylo-hexose (D-4dxylHex), was found in the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582. D-4dxylHex was isolated in the free state by paper chromatography after full acid hydrolysis of the polysaccharide and identified by GLC-mass spectrometry, 1H and 13C NMR spectroscopy, and specific rotation. It occurs as a lateral substituent in ∼40% of the oligosaccharide repeating units, making the polysaccharide devoid of strict regularity. The structure of the polysaccharide was established by sugar analysis, Smith degradation, and two-dimensional 1H and 13C NMR spectroscopy. In addition, a minor polysaccharide was isolated from the same lipopolysaccharide and found to contain 4-O-methylrhamnose.
  Journal of Natural Products 09/2011; 74(10):2161-7. · 3.13 Impact Factor
• Article: Structural and functional peculiarities of the lipopolysaccharide of Azospirillum brasilense SR55, isolated from the roots of Triticum durum.

  Alevtina S Boyko, Svetlana A Konnova, Yulia P Fedonenko, Evelina L Zdorovenko, Olga N Smol'kina, Vadim V Kachala, Vladimir V Ignatov
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  ABSTRACT: Azospirillum brasilense SR55, isolated from the rhizosphere of Triticum durum, was classified as serogroup II on the basis of serological tests. Such serogroup affiliation is uncharacteristic of wheat-associated Azospirillum species. The lipid A of A. brasilense SR55 lipopolysaccharide contained 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, hexadecanoic and octadecenoic fatty acids. The structure of the lipopolysaccharide's O polysaccharide was established, with the branched octasaccharide repeating unit being represented by l-rhamnose, l-3-O-Me-rhamnose, d-galactose and d-glucuronic acid. The SR55 lipopolysaccharide induced deformations of wheat root hairs. The lipopolysaccharide was not involved in bacterial cell aggregation, but its use to pretreat wheat roots was conducive to cell adsorption. This study shows that Azospirillum bacteria can utilise their own lipopolysaccharide as a carbon source, which may give them an advantage in competitive natural environments.
  Microbiological Research 02/2011; 166(7):585-93. · 2.31 Impact Factor
• Article: Structure of the O-polysaccharide of Pragia fontium 27480 containing 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid.

  Olga A Valueva, Evelina L Zdorovenko, Vadim V Kachala, Liudmyla D Varbanets, Nikolay P Arbatsky, Vladimir V Shubchynskyy, Alexander S Shashkov, Yuriy A Knirel
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  ABSTRACT: The following structure of the O-polysaccharide of Pragia fontium 27480 was elucidated by sugar analysis, including determination of the absolute configurations of the monosaccharides, and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-β-d-ManpNAc3NAcA-(1→2)-α-l-Rhap-(1→3)-β-l-Rhap-(1→4)-α-d-GlcpNAc-(1→ where ManNAc3NAcA stands for 2,3-diacetamido-2,3-dideoxymannuronic acid.
  Carbohydrate research 01/2011; 346(1):146-9. · 2.03 Impact Factor
• Article: Structure of the O-polysaccharide of the lipopolysaccharide of Pragia fontium 97U116.

  Evelina L Zdorovenko, Olga A Valueva, Liudmyla D Varbanets, Vladimir V Shubchinskiy, Alexander S Shashkov, Yuriy A Knirel
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  ABSTRACT: The O-polysaccharide of Pragia fontium 97U116 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide-repeating unit was established: -->2)-alpha-d-Galf-(1-->3)-alpha-l-Rhap2Ac(I)-(1-->4)-alpha-d-GlcpNAc(I)-(1-->2)-alpha-l-Rhap(II)-(1-->3)-beta-d-GlcpNAc(II)-(1-->
  Carbohydrate research 08/2010; 345(12):1812-5. · 2.03 Impact Factor
• Article: Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacteriumbifidum BIM B-465.

  Evelina L Zdorovenko, Vadim V Kachala, Anastasia V Sidarenka, Anastasia V Izhik, Elena P Kisileva, Alexander S Shashkov, Galina I Novik, Yuriy A Knirel
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  ABSTRACT: The composition and structure of cell wall polysaccharides of a biotechnologically promising probiotic strain of bifidobacteria Bifidobacteriumbifidum BIM B-465 were established by monosaccharide and methylation analyses along with 2D (1)H-(1)H homonuclear and (1)H-(13)C heteronuclear correlation NMR spectroscopy. The major polysaccharide represents a branched glucogalactan consisting of heptasaccharide repeating units having the following structure: -->6)-alpha-D-Glcp-(1-->3)-beta-D-Galf-(1-->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->3)-alpha-D-Galp-(1-->3)-alpha-D-Glcp-(1--> [structure: see the text]. The second isolated polysaccharide is a branched glucan with the main chain of (1-->6)-linked alpha-d-glucopyranose residues, approximately 60% of which are 2-substituted with a single alpha-d-glucopyranosyl group. This polysaccharide is a characteristic of various microorganisms, including some lactobacteria studied earlier.
  Carbohydrate research 09/2009; 344(17):2417-20. · 2.03 Impact Factor
• Article: Structure of the O-polysaccharides of the lipopolysaccharides of Mesorhizobium loti HAMBI 1148 and Mesorhizobium amorphae ATCC 19655 containing two O-methylated monosaccharides.

  Evelina L Zdorovenko, Olga A Valueva, Vadim V Kachala, Alexander S Shashkov, Nina A Kocharova, Yuriy A Knirel, Jolanta Kutkowska, Anna Turska-Szewczuk, Teresa Urbanik-Sypniewska, Adam Choma, Ryszard Russa
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  ABSTRACT: The O-polysaccharide of Mesorhizobium loti HAMBI 1148 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation, and (1)H and (13)C NMR spectroscopies, including 2D (1)H/(1)H COSY, TOCSY, ROESY, and H-detected (1)H/(13)C HSQC experiments. The O-polysaccharide was found to have a branched hexasaccharide-repeating unit of the following structure: [Formula: see text] where 2-acetamido-2-deoxy-4-O-methyl-D-glucose (D-GlcNAc4Me) and methyl group on 2-substituted D-rhamnose (Me) shown in italics are present in approximately 80% and approximately 40% repeating units, respectively. Similar studies of the O-polysaccharide from Mesorhizobium amorphae ATCC 19655 by sugar analysis and NMR spectroscopy revealed essentially the same structure but a higher content of 3-O-methyl-D-rhamnose ( approximately 70%).
  Carbohydrate research 09/2009; 344(18):2519-27. · 2.03 Impact Factor
• Article: Isolation and structure elucidation of two different polysaccharides from the lipopolysaccharide of Rahnella aquatilis 33071T.

  Evelina L Zdorovenko, Ludmila D Varbanets, George V Zatonsky, Galina M Zdorovenko, Alexander S Shashkov, Yuriy A Knirel
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  ABSTRACT: Two different polysaccharides were obtained by mild acid degradation of the lipopolysaccharide of Rahnella aquatilis 33071(T). These were studied by sugar and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structures were established for the polysaccharides: -->4)-alpha-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->3)beta-D-Galp-(1-->3)-alpha-D-Manp-(1-->2)-alpha-D-Manp-(1--> beta-D-Glcp-(1-->3)-alpha-D-galp-(1-->4)-alpha-D-GlcpA-(1-->2). The former structure is new, whereas the latter has been reported earlier as the structure of the O-specific polysaccharide of R. aquatilis 95 U003 (Zdorovenko, E. L.; Varbanets, L. D.; Zatonsky, G. V.; Kachala, V. V.; Zdorovenko, G. M.; Shashkov, A. S.; Knirel, Y. A. Carbohydr. Res.2008, 343, 2494-2497).
  Carbohydrate research 05/2009; 344(10):1259-62. · 2.03 Impact Factor
• Article: Structure of the O-specific polysaccharide of the lipopolysaccharide of Rahnella aquatilis 95 U003.

  Evelina L Zdorovenko, Lyudmyla D Varbanets, George V Zatonsky, Vadim V Kachala, Galina M Zdorovenko, Alexander S Shashkov, Yuriy A Knirel
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  ABSTRACT: The O-polysaccharide of Rahnella aquatilis 95 U003 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [carbohydrate structure: see text]
  Carbohydrate Research 10/2008; 343(14):2494-7. · 2.33 Impact Factor
• Article: Structural analysis of the O-antigen of the lipopolysaccharide from Azospirillum lipoferum SR65.

  Yuliya P Fedonenko, Evelina L Zdorovenko, Svetlana A Konnova, Vadim V Kachala, Vladimir V Ignatov
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  ABSTRACT: A neutral O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide isolated by phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum lipoferum SR65. The following structure of the O-polysaccharide was established by composition and methylation analyses, Smith degradation, and (1)H and (13)C NMR spectroscopy, including a 2D ROESY experiment: formula see text.
  Carbohydrate research 07/2008; 343(16):2841-4. · 2.03 Impact Factor
• Article: Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17.

  Yuliya P Fedonenko, Olga N Konnova, Evelina L Zdorovenko, Svetlana A Konnova, George V Zatonsky, Alexander S Shashkov, Vladimir V Ignatov, Yuriy A Knirel
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  ABSTRACT: A mixture of two structurally distinct neutral O-polysaccharides was obtained by mild acid degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense S17. The following structures of the O-polysaccharides were established by composition and methylation analyses, Smith degradation, and 1H and 13C NMR spectroscopy, including a 2D NOESY experiment: [Formula: see text] where L-Rha2Me stands for 2-O-methyl-L-rhamnose and SHb for the (S)-3-hydroxybutanoyl group. The occurrence of two distinct polysaccharides is reported for the first time in Azospirillum spp.
  Carbohydrate Research 04/2008; 343(4):810-6. · 2.33 Impact Factor
• Article: Structures of two putative O-specific polysaccharides from the Rahnella aquatilis 3-95 lipopolysaccharide.

  Evelina L Zdorovenko, Lyudmyla D Varbanets, George V Zatonsky, Andrey N Ostapchuk
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  ABSTRACT: Two polysaccharide preparations (OPSI and OPSII) were obtained by mild acid degradation of the lipopolysaccharide of Rahnella aquatilis 3-95. Studies by chemical methods and 1H and 13C NMR spectroscopy showed that OPSI is a linear alpha-D-mannan having a trisaccharide repeat and OPSII is a approximately 2:1 mixture of the same mannan and an alpha-d-glucan:
  Carbohydrate Research 02/2006; 341(1):164-8. · 2.33 Impact Factor
• Article: Structure of the O-polysaccharide from the Azospirillum lipoferum Sp59b lipopolysaccharide.

  Yuliya P Fedonenko, Olga N Konnova, George V Zatonsky, Svetlana A Konnova, Nina A Kocharova, Evelina L Zdorovenko, Vladimir V Ignatov
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  ABSTRACT: A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant-growth-promoting bacterium Azospirillum lipoferum Sp59b. On the basis of sugar and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy, including a NOESY experiment, the following structure of the branched hexasaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text].
  Carbohydrate Research 06/2005; 340(6):1259-63. · 2.33 Impact Factor