Anita Olejek

Medical University of Silesia in Katowice, Catowice, Silesian Voivodeship, Poland

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Publications (75)62.53 Total impact

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    ABSTRACT: Vulvar cancer accounts for about 3-5% of all female genital carcinomas. TGF-P protein is a member of a superfamily of cytokines that regulate cell functions. A correlation between this protein and many neoplastic processes was reported. In our study we analyzed TGF-β expression in vulvar tumor among patients with diagnosed squamous cell carcinoma (with and without inguinal nodes metastases). Paraffin embedded blocks obtained from vulvar tissues and inguinal nodes (from 31 patients with vulvar carcinoma FIGO ll-IV) were prepared. Next, the hematoxylin and eosin staining was performed. Monoclonal antibody NCL-TGF-beta was used for immunohistochemical tests. Higher expression of TGF-beta in cancer cells corresponds to more advanced cancer stages (FIGO). A positive correlation between TGF-beta and metastases, as well as a number of inguinal nodes metastases was observed. The ratio between the number of stained cells in vulvar tumor and of inflammatory cells proved to be higher in FIGO stage III than IV Possibly TGF-beta increase in vulvar tumor contributes to the breakdown of immunological processes limiting cancer progression. Higher TGF-beta expression leads to metastasis in regional lymphatic nodes. TGF-beta overproduction is observed in vulvar neoplastic processes. In early stages of carcinogenesis TGF-beta inhibits cancer cell proliferation, but in more advanced stages it accelerates cancer progression by inhibiting the immunological response.
    Ginekologia polska 11/2014; 85(11):847-51. DOI:10.17772/gp/1912
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    ABSTRACT: The study aimed at investigating the impact of late prematurity (LPT) on neonatal outcome in twins and neonatal morbidity and mortality within LPT with regard to the completed weeks of gestation. The study was conducted in six tertiary obstetric departments from different provinces of Poland (Warsaw, Lublin, Poznan, Wroclaw, Bytom). It included 465 twin deliveries in the above centers in 2012. A comparative analysis of maternal factors, the course of pregnancy and delivery and neonatal outcome between LPT (34 + 0-36 + 6 weeks of gestation) and term groups (completed 37 weeks) was performed. The neonatal outcome included short-term morbidities. The analysis of neonatal complication rates according to completed gestational weeks was carried out. Out of 465 twin deliveries 213 (44.8%) were LPT and 156 (33.55%) were term. There were no neonatal deaths among LPT and term twins. One-third of LPT newborns suffered from respiratory disorders or required antibiotics, 40% had jaundice requiring phototherapy, and 30% were admitted to NICU. The analysis of neonatal morbidity with regard to each gestational week at delivery showed that most analyzed complications occurred less frequently with the advancing gestational age, especially respiratory disorders and NICU admissions. The only two factors with significant influence on neonatal morbidity rate were neonatal birth weight (OR = 0.43, 95% CI = 0.2-0.9, p = .02) and gestational age at delivery (OR = 0.62, 95% CI = 0.5-0.8, p < .01). LPT have a higher risk of neonatal morbidity than term twins. Gestational age and neonatal birth weight seem to play a crucial role in neonatal outcome in twins.
    Twin Research and Human Genetics 08/2014; 17(5):1-7. DOI:10.1017/thg.2014.48
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    Iwona Gabriel, Anita Olejek
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    ABSTRACT: Women using hormonal therapy for breast cancer are often encountered in clinical practice of breast surgeons, oncologists and gynecologists. Some of them during the course of therapy develop abnormal uterine bleeding or have ultrasound abnormalities detected. In Poland, most of them are still diagnosed using blind curettage, which does not bring a definitive diagnosis or requires repeating the diagnostic procedure. This produces unnecessary fear of malignancy in patients and increases economic costs (double curettage, unnecessary hysterectomy and its social consequences). Therefore, we studied the usefulness of hysteroscopy with targeted biopsies for further management of women treated with tamoxifen. Our goal is to provide evidence that women before entering, during and after tamoxifen treatment have hysteroscopy for endometrial assessment.
    Videosurgery and Other Miniinvasive Techniques / Wideochirurgia i Inne Techniki Malo Inwazyjne 06/2014; 9(2):258-61. DOI:10.5114/wiitm.2013.40106
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    ABSTRACT: The aim of the study was to determine a relation between the level of total serum bile acids in pregnant women and the condition of their newborns on the basis of select parameters of acid-base balance in the cord blood. The study group consisted of 64 pregnant women with Intrahepatic Cholestasis of Pregnancy and 78 healthy pregnant women constituted the control group. Colorimetric enzymatic measurements of serum total bile acids were performed in both groups. In the mild cholestasis group, mean concentration of bile acids amounted to 20.6 +/- 1.0 micromol/l and was statistically significantly lower as compared to women with severe cholestasis (58.9 +/- 9.5 micromol/l). In the control group, mean concentration of bile acids was 4.1 +/- 0.3 micromol/l and was statistically significantly lower as compared to groups with cholestasis. Caesarean section was performed in 60.9% of women with cholestasis as compared to 19.2% of controls. Also, neonatal condition in the first minute of life was worse in the group with cholestasis as compared to the infants in the control group. The body weight of newborns of mothers with cholestasis was statistically significantly lower than in infants born to controls. Lowered neonatal pH (below 7.20) was noted in 2.6% of the newborns from the control group and was statistically significantly less frequent as compared to infants from the group with cholestasis. Periodic measurements of total bile acid concentrations in serum of pregnant women with cholestasis is of high value in establishing the diagnosis and determining severity of the disease in the mother as well as potential hazard for the fetus. Simultaneous monitoring of the changes total bile acids concentrations in the blood serum and the well-being allows to reduce the rates of neonatal distresses after birth and cesarean sections.
    Ginekologia polska 02/2014; 85(2):101-4. DOI:10.17772/gp/1698
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    ABSTRACT: Diabetes mellitus, as a risk factor for endometrial cancer (EC), causes an increase in insulin and IGF-1 concentrations in the blood serum. The increase in insulin and IGF-1 are considered mitogenic factors contributory to cancer development. Studies suggest that metformin has preventive activity, decreasing mortality and the risk of neoplasms. Since estrogen (ER), progesterone (PR) and IGF-1 (IGF-1R) receptor expression and beta-catenin and PAX-2 mutations are significant in the development of endometrial cancer, it was decided to study these factors in patients with endometrial cancer and type 2 diabetes mellitus (DM2), and to establish the effects of metformin on their expression. The expression of ER, PR, IGF-1R, beta-catenin and PAX-2 have been immunohistochemically investigated in 86 type I endometrial cancer specimens. Patients were grouped according to the presence of DM2 and the type of hypoglycemic treatment administered. Comparing EC patients with DM2 and normal glycemic status, we found increased IGF-1R expression in women with DM2. A decrease in ER expression was noted in women with EC and DM2 receiving metformin as compared to women treated with insulin (p = 0.004). There was no statistically significant difference in PR, IGF-1R, beta-catenin and PAX-2 expression among women receiving metformin and other hypoglycemic treatment. Although epidemiological studies suggest the beneficial role of metformin in many human cancers, there are still few studies confirming its favorable effect on endometrial cancer. Decreased ER expression in patients receiving metformin needs further research to allow evaluation of its clinical significance.
    Diabetology and Metabolic Syndrome 12/2013; 5(1):76. DOI:10.1186/1758-5996-5-76
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    ABSTRACT: Abstract Objective: The aim of the study was a randomized comparison of the outcomes of intrauterine myelomeningocele repairs (IUMR) in type II Chiari malformation (II CM) fetuses with clinical data of newborns and infants operated on postnatally. Methods: The study group (SG) comprised of 46 pregnant women whose type II CM children underwent IUMR, while 47 pregnant women whose type II CM children were operated on postnatally constituted the control group (CG). A total of 24 SG and 20 CG patients reached the endpoint of the study. Results: High incidence of PROM (24 (52.2%), CI 3.74 (1.69-8.26) (p<0.001) was noted in the group of prenatal surgeries as compared to controls. The need for VP shunt implantation was statistically significantly lower (p<0.008) in the group of children after IUMR as compared to controls (5 (27.8%) and 16 (80%), respectively, CI 0.35 (0.16-0.75). None of the postnatally treated CG children can walk without adaptive equipment. In contrast, two children from the study group (2 (11.1%) CI 1.86 (1.00-3.48) p<0.048) are able to walk independently. Conclusions: Prenatal MMC closure significantly lowers further adverse evolution of the type II Chiari malformation. Further studies are needed, especially on preventive measures for preterm labor and IPPROM in the postoperative course of IUMR.
    The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 10/2013; 27(14). DOI:10.3109/14767058.2013.858689
  • 05/2013; 11(1):9-23. DOI:10.15557/CGO.2013.0001
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    ABSTRACT: Marfan syndrome is an autosomal dominant disorder of connective tissue with up to 25% of cases related to a spontaneous mutation. It has been associated with perinatal loss, preterm labor and, potentially a rupture of the maternal aortic arch. We present a case of a woman diagnosed with Marfan syndrome after a miscarriage of her first pregnancy. At the time of diagnosis she had mild aortic bulb dilation and insufficiency of the mitral and tricuspid valves. She underwent cardiosurgical correction, after which she had two uneventful pregnancies. This case suggests that preconceptional correction of valve defects in women with Marfan syndrome may decrease the risk of cardiac decompensation during future pregnancies. Additionally close clinical follow up and the appropriate use of beta-adrenergic blockade may decrease the risk of aortic rupture, a significant risk factor for mortality in pregnant women.
    Ginekologia polska 04/2013; 84(4):314-7. DOI:10.17772/gp/1583
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    ABSTRACT: Vaginal cancer is a rare neoplasm and in about 65% to 90% of cases it is a metastatic one. It is diagnosed in women aged from 60 to 70 years, but it can also appear in younger patients. Squamous cell carcinoma is the most frequently found histopathological type. Risk factors for the development of vaginal cancer are thought to be similar to those of cervical cancer. We present a case of a 52-year old woman with vaginal cancer treated with brachytherapy. Due to neoplasm recurrence, additional examinations were performed in the course of one year. The patient was qualified for total pelvic exenteration. A leakage from the abdominal wound was observed post operatively. It was successfully noninvasively treated. In 3 months time she was hospitalized because of the superficial abdominal abscess which was located next to the left ureterostomy. The patient undergoes regular gynecological, surgical and urological control examinations. So far the results do not show any recurrence of the neoplastic process. Total pelvic exenteration should be considered in selected groups of patients.
    Ginekologia polska 02/2013; 84(2):142-6. DOI:10.17772/gp/1555
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    ABSTRACT: We present case report of 51-year-old female patient admitted to surgery ward because of presence of pyogenic discharge in perianal region since 7 months. Eighteen months earlierthe patient underwent stress urinary incontinence procedure with use of TVT synthetic implant. Diagnosis of wast, bilateral, composite vagino-perianal fistule was made. After carrying out four operations with two stage sling removal final postfistule wound healing was obtained.
    Wiadomości lekarskie (Warsaw, Poland: 1960) 01/2013; 66(2 Pt 2):206-9.
  • Menopausal Review 01/2013; 2:155-158. DOI:10.5114/pm.2013.35075
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    ABSTRACT: The present study has focused on the identification of the differences between expression patterns of kinin-dependent genes in endometrial cancer with the use of real-time quantitative reverse transcription polymerase chain reaction and oligonucleotide microarray. The study group consisted of 50 endometrium samples collected from women with endometrial cancer. Gene expression of kinin receptors BR1 and BR2 was evaluated with real-time quantitative reverse transcription polymerase chain reaction. The analysis of the expression profile of genes related to the kinin mitogenic signal transduction pathway was performed using HG-U133A oligonucleotide microarrays. The transcriptional activity of the B1 receptor for kinins increased in patients with grade 1 (G1) and grade 2 (G2) endometrial cancer when compared to the control group, whereas it decreased in patients with grade 3 (G3) endometrial cancer. The expression of the B2 receptor showed a growing trend reaching the peak in the G2, whereas G3 was characterized by a decrease in the gene transcriptional activity. Significant differential gene expression was recorded for GNB1, PRKAR1A, KRAS, MAP2K2, GNG5, MAPK1, ADCY9, GNG11, JUN, PRKCA, PRKACB, FOS, PLCB4, ADCY8, and GNG12. The expression changes in kinin-dependent genes might cause disturbance in the underlying biological processes, which could be important for the pathogenesis of endometrial cancer. This will eventually help to improve treatment strategies for patients with endometrial cancer in the future.
    International Journal of Gynecological Cancer 06/2012; 22(6):937-44. DOI:10.1097/IGC.0b013e318259d8da
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    ABSTRACT: Endometrial cancer is one of the most frequently diagnosed malignant neoplasms among women. In Poland, it is in the fourth place in terms of incidence. The highest morbidity concerns women aged 50-70 years, however it may also appear in women in their reproductive period. Endometrial cancer concerns about 3% of premenopausal women. We present a case of a 25-year-old patient who underwent endometrial curettage because of irregular menstrual bleeding for the last 5 months. Histopathology revealed endometrial cancer. We attempted to apply a conservative treatment. During the next 6 months the patient was treated with lynestrenol. After one month of hormonal therapy endometrial curettage was repeated. In histopathology endometrial tissues corresponding to the hormonal treatment were found. After 6 months of treatment hysteroscopy with endometrial biopsy followed by endometrial curettage, were performed. Hormonal treatment resulted in disease regression. About 5 months after successful treatment the patient conceived spontaneously. One year after she gave birth to her first child, she conceived spontaneously once more. Both children were born vaginally. In selected cases of atypical hyperplasia and early endometrial cancer in young women the attempt of hormonal treatment is acceptable.
    Ginekologia polska 03/2012; 83(3):224-8.
  • Menopausal Review 01/2012; 4:275-280. DOI:10.5114/pm.2012.30229
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    ABSTRACT: Cyclopia is a deformation of the facial skeleton with one eye orbit formed in the place where both eyes should be present. As a result of hypoplasia there is absence (hypothesized loss) of central nervous system structures. Teratogenic factors leading to the occurrence of this anomaly may include irregular cholesterol biosynthesis, viruses, alcohol intake and maternal diabetes. Many authors suggest genetic etiology of this illness. The following work presents a case of a female patient whose fetus was diagnosed with multiple defects, among others with cyclopia. After pharmacological induction of labor, a male fetus with vital signs was born but died after two hours. As far as cyclopia is concerned, special attention should be paid to proper diagnosis of this pathology at an earliest possible stage of fetal life. Early ultrasound diagnostics of this anomaly must be emphasized most strongly leading to the conclusion that patients suspected of fetal facial skeleton defects should be referred to medical centers which are qualified in prenatal examinations.
    Ginekologia polska 03/2011; 82(3):221-5.
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    ABSTRACT: Lichen sclerosus (LS) is a chronic skin and mucosa inflammatory disease. It affects mainly the female anogenital area especially in postmenopausal period. The main symptoms include pruritus, burning, pain, sometimes urinary problems, or difficulties in defecation. Usually, porcelain-white plaques are seen in the skin and mucosa. The etiology and pathogenesis of LS are still uncertain. There are some research studies on possible genetic predisposition, yet autoimmune, hormonal, or infectious factors are not excluded. The typical treatment of LS is mainly pharmacological, although the alternative treatment method used in LS is photodynamic therapy (PDT), which is noninvasive technique based on selective destruction of lesions. Our study is focused on molecule markers of vascularisation (CD34), nervous cell function (myelin basic protein [MBP]), keratinocyte function (CD44), and proliferation index (Ki67) in cases treated with photodynamic method. A group of 100 patients treated in our department was included in the study. All 100 women had LS on the basis of clinical and histological criteria. All the subjects underwent PDT. In all cases, skin biopsies were taken before and after treatment, and samples were analyzed with CD34, CD44, MBP, and Ki67 antibodies using immunohistochemical staining. The study shows the high efficacy of PDT in LS treatment including beneficial changes to CD34, CD44, and MBP immunostained molecules. The Ki67 proliferation index did not change significantly. A significant increase of CD34 (microvessel density), MBP, and CD44 expression was confirmed in the histological images and in the partial or full remission of clinical objective and subjective symptoms. The PDT is a very effective therapeutic method in LS treatment.
    International Journal of Gynecological Cancer 07/2010; 20(5):879-87. DOI:10.1111/IGC.0b013e3181d94f05
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    ABSTRACT: Heat shock proteins (Hsp) are highly expressed in many malignant human tumors, including tumors of the genital tract. One of the Hsps strongly associated with the process of cancerogenesis is Hsp27. The presence of Hsp27 and anti-Hsp27 in the sera of patients with ovarian carcinoma is still a new research field, and papers contain contradictory results. The aim of this work was to study the concentrations of anti-Hsp27 immunoglobulin G antibodies in the sera of women with ovarian cancer at different clinical stages and with different histopathological types of this cancer. Serum samples from 158 patients with ovarian carcinoma and 80 healthy women were investigated. The concentrations of anti-Hsp27 antibodies were determined by the enzyme-linked immunosorbent assay technique. The mean concentration of anti-Hsp27 antibodies in the patients with ovarian carcinoma was significantly higher than that in the control group. Analysis in relation to the stage of clinical progression showed that the less advanced the cancerogenesis process, the higher the concentration of the anti-Hsp27 antibodies is. The mean concentrations of the anti-Hsp27 antibodies in the patients with ovarian carcinoma were not significantly different in relation to the histological type of the cancer. The use of chemotherapy as a primary anticancer treatment in ovarian carcinoma did not cause a significant decrease in the concentration of anti-Hsp27 antibodies. An immunological response to Hsp27 is increased in women with ovarian carcinoma. Although the diagnostic concentrations of anti-Hsp27 antibodies have not been precisely defined yet, we believe that this may be a helpful diagnostic parameter particularly to detect early stages of clinical advancement of the disease.
    International Journal of Gynecological Cancer 12/2009; 19(9):1516-20. DOI:10.1111/IGC.0b013e3181bf425b
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    ABSTRACT: Vulvovaginitis is the most common cause of gynecological complaints in young girls. Factors which cause vulvovaginitis include, among other things, low level of sexual hormones (hypoestrogenism), the anatomical proximity of the rectum and delicate vulvar skin and vaginal mucosa. Usually vulvovaginitis in young girls is caused by non-specific factors. The aim of the study was to present the most frequent causes of vulvovaginitis in young girls.
    Ginekologia polska 12/2009; 80(12):931-4.
  • Ginekologia polska 11/2009; 80(11):870-6.
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    ABSTRACT: A germinal matrix is an area of the fetal brain where neuroblasts proliferate till the 20th week of gestation after which their migration takes place. Germinal matrix hemorrhage (GMH) is a frequent cause of fetal ventriculomegaly or hydrocephalus, yet the pathophysiological conditions of GMH remain unclear. A case of fetal ventriculomegaly resulting from GMH has been reported in the article. A prenatal ultrasonographic examination (US) carried out in the 30th week of gestation revealed enlarged ventricles. Utero magnetic resonance imaging (MRI) performed in the 34th week of gestation demonstrated asymetric ventriculomegaly and the changes suggesting the occurance of GMH and ependymal intraventricular hemorrhage. Fetal ventriculomegaly and hydrocephalus are commonly detected by ultrasonographic examination, but it is often difficult to determine the causes of the diseases by US. In these cases MRI allows to establish the final diagnosis.
    Ginekologia polska 10/2009; 80(10):782-5.

Publication Stats

175 Citations
62.53 Total Impact Points

Institutions

  • 2001–2014
    • Medical University of Silesia in Katowice
      • Department of Obstetrics and Gynecology
      Catowice, Silesian Voivodeship, Poland
  • 2012–2013
    • Pomorski Uniwersytet Medyczny
      Stettin, West Pomeranian Voivodeship, Poland
  • 2005–2013
    • Silesian University of Technology
      Gleiwitz, Silesian Voivodeship, Poland