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ABSTRACT: A Gram-staining-negative, non-endospore-forming, yellow pigmented strain (E89T) was isolated from the skin of the medical leech Hirudo verbana obtained from the leech farm located in Biebertal, Germany. 16S rRNA gene sequencing analysis showed that the isolate was grouped into the genus Flavobacterium. Strain E89T was most closely related to the type strains of Flavobacterium chilense (98.2%), Flavobacterium chungangense (98.1%), and 'Flavobacterium oncorhynchi' (98.1%). To all other Flavobacterium species 16S rRNA gene sequence similarities were ≤ 97.4%. A menaquinone from the type MK-6 was found to be the predominant respiratory quinone and the polar lipid profile consisted of the major compounds phosphatidylethanolamine, phosphatidylserine, two unidentified aminolipids, one unknown phospholipid and two unknown lipids. The fatty acid profiles was composed of iso C15:0, C15:0, summed feature 3 (C16:1 ω7c and/or iso C15:0 2-OH) found in major amounts and several hydroxylated fatty acids in smaller amounts, among them iso C16:0 3-OH, iso C17:0 3-OH, C16:0 3-OH and C15:0 3-OH. All these data support the allocation of the isolate into the genus Flavobacterium. Physiological/biochemical characterisation and DNA-DNA hybridizations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of the strain. For this reason, we propose for strain E89T (= DSM 25795T = LMG 26922T = CIP 110374T) a new species with the name Flavobacterium cutihirudinis sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
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ABSTRACT: Three pink-pigmented, aerobic, Gram-negative, rod-shaped and facultatively methylotrophic strains were isolated from the phyllosphere of Trifolium repens and Cerastium holosteoides. 16S rRNA gene sequence analysis support the affiliation of all strains to the genus Methylobacterium. The closest relatives of strains C34T and T5 were Methylobacterium gnaphalii 23eT (98.0 and 98.5%, respectively) and M. organophilum JCM 2833T (97.0 and 97.2%, respectively). Strain TA73T showed the highest sequence similarities to M. marchantiae JT1T and M. bullatum F3.2T (both 97.9%), followed by M. phyllosphaerae CBMB27T and M. brachiatum DSM 19569T (both 97.8%), M. cerastii C15T and M. radiotolerans JCM 2831T (both 97.7%). The major components in the fatty acids profile were C18:1 ω7c, C16:0 and one unknown fatty acid for strain TA73T and C18:1ω7c, C16:1ω7c/C15:0 iso 2-OH, C18:0 and C16:0 for strains C34T and T5. Physiological and biochemical analysis, including DNA-DNA hybridization, revealed clear differences between the investigated strains and their closest phylogenetic neighbours. But DNA-DNA hybridization studies also showed high similarities between strains C34T and T5 (59.6 - 100%). Therefore, the isolates represent two novel species within the genus Methylobacterium, for which the names M. trifolii (type strain TA73T = DSM 23632T = CCM 7786T) and M. thuringiense (type strain C34T = DSM 23674T = CCM 7787T) are proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
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ABSTRACT: A Gram-positive, non spore forming actinobacterium (01-Gi-040T) isolated from an indoor wall was studied for its taxonomic position. The isolate formed a very rudimentary substrate-mycelium that fragments into rod-shaped to coccoid cells. On the basis of the 16S rRNA gene sequence similarity studies, strain 01-Gi-040T was shown to belong to the genus Ornithinimicrobium closely related to Ornithinimicrobium kibberense DSM 17687T (97.1 %), Ornithinimicrobium humiphilum DSM 12362T (96.2 %) and Ornithinimicrobium pekingense GCMCC1.5362T (96.1%). A close relationship was also found with Arsenicicoccus bolidensis DSM 15362T (95.9%) and Arsenicicoccus piscis Kis4-19T (95.7%) and a moderate relationship to the type strains of the genus Serinicoccus (94.0 to 94.1%) The predominant menaquinone of strain 01-Gi-040T was MK-8(H4). The peptidoglycan contained ornithine as diagnostic diamino acid. The polar lipid profile consisted of the lipids phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, an unknown aminolipid and two unknown phosphoglycolipids. The major fatty acids iso-C15:0, iso-C16:0, and iso-C17:0 were consistent with the fatty acid patterns reported for Ornithinimicrobium species. The results of DNA-DNA hybridizations, physiological and biochemical tests allowed a phenotypic differentiation of strain 01-Gi-040T from the three known Ornithinimicrobium species. Strain 01-Gi-040T represents a novel Ornithinimicrobium species, for which we propose the name Ornithinimicrobium murale sp. nov., with the type strain 01-Gi-040T (= DSM 22056T = CCM 7610T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2012; · 2.11 Impact Factor
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ABSTRACT: A beige-coloured, Gram-stain negative, aerobic, non-motile moderately thermotolerant, rod-shaped organism, strain CC-SPIO-10-1T, was isolated from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan, on Marine Agar 2216. Based on the 16S rRNA gene sequence analysis this organism was grouped into genus Stappia, showing 98.3% sequence similarity to Stappia indica and 98.2% gene sequence similarity to Stappia stellulata. Ubiquinone Q-10 was the major respiratory quinone and C18:1 ω7c and C18:1ω7c 11-methyl were detected as the major fatty acids. The hydroxylated fatty acid C18:0 3-OH was detected as well. Predominant polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, unidentified aminolipid AL1 and unidentified phospholipid PL1. Minor amounts of several unidentified lipids (PL2, L1-L7) are present as well. The polyamine pattern contains the major compounds spermidine and spermine. Strain CC-SPIO-10-1T could be differentiated from the type strains of S. stellulata and S. indica by a set of biochemical tests. On the basis of the 16S rRNA gene sequence analysis and of the chemotaxonomic and physiological data, strain CC-SPIO-10T represents a new species of the genus Stappia for which the name Stappia taiwanensis sp. nov. is proposed. The type strain is CC-SPIO-10 T (= CCUG 59208T = LMG 25538 T = CCM 7757T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2012; · 2.11 Impact Factor
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ABSTRACT: Two Gram-positive non endospore-forming rods, C5T and T8T, were isolated from the phyllosphere of Cerastium holosteoides and Trifolium repens, respectively, and were studied in detail for their taxonomic position. 16S rRNA gene sequence analysis allocated both isolates clearly to the genus Rhodococcus. Isolate C5T was most closely related to Rhodococcus fascians and Rhodococcus yunnanensis, showing 99.2% gene sequence similarity to both species. Strain T8T revealed the highest 16S rRNA gene sequence similarity to Rhodococcus corynebacterioides (98.8%) and Rhodococcus kroppenstedtii (98.6%). The quinone system of both strains is composed of dihydroginated menaquinones with 8 (major amount) as well as 9, 7 and 6 isoprenoid units (MK-8H2, MK-9H2 MK-7H2 MK-6H2).The polar lipid profiles of strains C5T and T8T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. Additionally, C5T contained one unknown glycolipid, and T8T three unknown aminolipids. The fatty acids profiles contained major amounts of C16:0, C18:1 w9c and 10-methyl C18:0, which supported the grouping of the two isolates to the genus Rhodococcus. Physiological/biochemical characterisation and DNA-DNA hybridizations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of both strains. For this reason, we propose for strain C5T (= LMG 26203T = CCM 7906T) a new species with the name Rhodococcus cerastii sp. nov., and for strain T8T (= LMG 26204T = CCM 7905T) a second new species with the name Rhodococcus trifolii sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-staining negative, rod-shaped, non-spore-forming bacterium isolated as a contaminant from a biopharmaceutical process (strain CCUG 53591T ) was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence similarity this strain was clearly allocated to the genus Herminiimonas. Herminiimonas saxobsidens was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (99.9%), followed by Herminiimonas glaciei (99.7%) and Herminiimonas arsenicoxidans (98.8%). Strain ND5, previously reported as H. glaciei, but showing 100% 16S rRNA gene-sequence similarity to strain CCUG 53591T was included in the comparative study. Similarities to all other Herminiimonas species were below 98.0%. Chemotaxonomic data (major ubiquinone: Q-8; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acids C17:0 cyclo, C19:0 cyclo ω8c, and C16:0, with C10:0 3-OH as hydroxylated fatty acid supported the affiliation of the isolate to Herminiimonas. DNA-DNA hybridization results (mean values) of strain CCUG 53591T with the type strains of H. saxobsidens CCUG 59860T (34%), H. glaciei DSM 21140T (57%), H. arsenicoxidans DSM 17148T (17%) and Herminiimonas fonticola S-94T (11%) clearly supported the separate taxonomic position of this strain. Strain ND5 showed DNA-DNA similarities of 78%, 56%, and 52% to strain CCUG 53591T, H. glaciei DSM 21140T and H. saxobsidens CCUG 59860T, respectively. A phenotypic differentiation of the isolate from the most closely related species was possible by different features. Hence strain CCUG 53591T represents a novel species, for which the name Herminiimonas contaminans sp. nov. is proposed, with the type strain CCUG 53591T (= CCM 7991T). Strain ND5 is a second strain of this species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-positive rod shaped bacterium (01-GI-001T) isolated from a wall colonized with moulds was studied for its taxonomic position. The 16S rRNA gene sequence analysis showed, that the isolate clearly grouped into the Microbacterium cluster. On the basis of pairwise comparisons of the 16S rRNA gene sequences, isolate 01-Gi-001T was most closely related to the type strains of Microbacterium hydrocarbonoxydans (98.8%), Microbacterium profundi (98.7%), Microbacterium phyllosphaerae (98.3%) and Microbacterium foliorum (98.1%). The diagnostic diamino acid of the peptidoglycan was ornithine. The detected major menaquinones were MK-13 and MK-12. The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, one unknown phospholipid and one unknown glycolipid. The fatty acid profile was mainly composed of anteiso-C15:0, iso-C16:0, and anteiso-C17:0 and was in congruence with the fatty acid patterns reported for other Microbacterium species. The results of physiological and biochemical tests and DNA-DNA hybridizations of strain 01-Gi-001T with the type strains of the most closely related species M. hydrocarbonoxydans, M. profundi, M. phyllosphaerae, and M. foliorum showed clear differences. For this reason, we propose for strain 01-Gi-001T (= DSM 22178T = CCM 7640T) a new species, with the name Microbacterium murale sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2011; · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, non-spore-forming rod (CC-LN1-12(T)) was isolated from coastal soil samples of Lutao Island (Green Island), Taiwan, and its taxonomic position was studied. 16S rRNA gene sequence analysis showed that isolate CC-LN1-12(T) was grouped into the Microbulbifer cluster, with the highest similarities to Microbulbifer okinawensis ABABA23(T) (97.9 %), Microbulbifer maritimus TF-17(T) (97.7 %) and Microbulbifer donghaiensis CN85(T) (97.7 %), similarities to all other species of the genus Microbulbifer were lower than 96.8 %. The polyamine pattern contained the major compounds spermidine and cadaverine. The fatty acid profile, comprising the major fatty acids iso-C(15 : 0), iso-C(17 : 1)ω9c, C(18 : 1)ω7c and iso-C(11 : 0) 3-OH as the major hydroxylated fatty acid, supported the affiliation of strain CC-LN1-12(T) to the genus Microbulbifer. DNA-DNA hybridizations between strain CC-LN1-12(T) and Microbulbifer okinawensis ABABA23(T), M. donghaiensis CN85(T) and M. maritimus JCM 12187(T) resulted in relatedness values of 21.5 % (14.3 %, reciprocal analysis), 35.9 % (48.5 %, reciprocal analysis) and 48.1 % (52.1 %, reciprocal analysis), respectively. From these data, as well as from physiological and biochemical tests, strain CC-LN1-12(T) could be clearly differentiated from the most closely related species of the genus Microbulbifer. It is concluded that strain CC-LN1-12(T) represents a novel species, for which the name Microbulbifer taiwanensis sp. nov. is proposed. The type strain is CC-LN1-12(T) ( = LMG 26125(T) = CCM 7856(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2011; 62(Pt 10):2485-9. · 2.11 Impact Factor
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ABSTRACT: On the basis of cultivation-dependent (isolation on mineral salt medium supplemented with 0.5% methanol) and -independent (DGGE analysis) methods, we investigated the influence of the host plant species Trifolium repens and Cerastium holosteoides, three geographic locations and the land-use types meadow, mown pasture and pasture on the abundance and community composition of selected phyllosphere bacteria with emphasis on Methylobacterium species. Methylobacterium abundance was significantly higher on leaves of T. repens (mean value 2.0×10(7) CFU PPFM per g leaf) than on leaves of C. holosteoides (mean value 2.0×10(6) CFU per g leaf). Leaves from the sampling site Schorfheide-Chorin showed slightly lower Methylobacterium numbers than leaves of the other sampling sites. Land-use and sampling period had no consistent influence on Methylobacterium community size. Methylobacterium community composition was very similar over both sampling periods, all three sampling sites, all land-use types and both plant species. Moreover, no relationship between geographic and genetic distance was observed. Community composition of selected Proteobacteria was influenced by plant species, geographic location and land-use. Often, differences in community composition could be observed between meadows, mown pastures and pastures but not between different kinds of meadows (cutted once versus three times) and mown pastures (fertilized versus non-fertilized). The results also indicate, that whether there are differences between land-use types or not strongly depends on the investigated host plant species and ecosystem. Besides Methylobacterium, representatives of Methylophilus were detected. The results indicate that Methylobacterium species are generally abundant and stable members of the phyllosphere community whereas other genera occur more occasionally, and that Methylobacterium clearly dominates the methylotrophic phyllosphere community.
Systematic and Applied Microbiology 12/2011; 34(8):621-30. · 3.37 Impact Factor
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ABSTRACT: The taxonomy of strain CCUG 55240(T), a Gram-staining-positive, aerobic, endospore-forming bacterium that was isolated from a paper mill, was investigated using a polyphasic approach. In phylogenetic analysis based on 16S rRNA gene sequences, the novel strain was grouped with established members of the genus Paenibacillus and appeared most closely related to the type strains of Paenibacillus chinjuensis (93.7 % sequence similarity), P. elgii (93.7 %) and P. chitinolyticus (93.6 %). The levels of 16S rRNA gene sequence similarity with other species of the genus Paenibacillus, including the type species of the genus, Paenibacillus polymyxa, were all <93.5 %. The fatty acid profile of strain CCUG 55240(T), which showed a predominance of iso- and anteiso-branched fatty acids, supported the allocation of the strain to the genus Paenibacillus. Unusually high amounts of some iso-branched fatty acids, especially iso-C(15:0) and iso-C(16:0), allowed differentiation of strain CCUG 55240(T) from the most closely related species of the genus Paenibacillus. The diagnostic diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid, an unknown aminophosphoglycolipid and an unknown phospholipid. Spermidine was the major polyamine. The results of some physiological and biochemical tests also allowed the phenotypic differentiation of strain CCUG 55240(T) from the most closely related recognized species. On the basis of the phylogenetic, phenotypic and molecular evidence, strain CCUG 55240(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus chartarius sp. nov. is proposed. The type strain of the novel species is CCUG 55240(T) ( = CCM 7759(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 62(Pt 6):1342-7. · 2.11 Impact Factor
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ABSTRACT: A yellowish pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain CC-TBT-3(T)), was isolated on marine agar 2216 from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. 16S rRNA gene sequence analysis of strain CC-TBT-3(T) showed a relatively low similarity (<95.5 %) to representatives of the genera Novosphingobium, Sphingosinicella and Sphingomonas of the Sphingomonadaceae, with the most related strain being the type strain of Novosphingobium soli. In addition to the relatively low 16S rRNA gene sequence similarity to members of established species, the isolate also showed some unique chemotaxonomic features, including the presence of some glycolipids with unusual chromatographic behaviour. The major components of the polar lipid profile were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and three unidentified glycolipids. The major respiratory quinone was ubiquinone Q-10. The polyamine pattern was characterized by the triamine sym-homospermidine as a major component. Although the predominant fatty acids were C(18:1)ω7c and summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH), the isolate did not show the typical hydroxyl fatty acids, such as C(14:0) 2-OH, C(15:0) 2-OH and C(16:0) 2-OH, found in members of the genera Novosphingobium, Sphingomonas and Sphingosinicella, but showed instead high amounts of C(18:1) 2-OH (12.0 %). The DNA G+C content of strain CC-TBT-3(T) was 63.4 mol%. 16S rRNA gene sequence, chemotaxonomic and physiological analyses revealed that strain CC-TBT-3(T) represents a novel species in a new genus in the family Sphingomonadaceae for which the name Sphingomicrobium lutaoense gen. nov., sp. nov. is proposed; the type strain is of the type species S. lutoaense, CC-TBT-3(T) ( = DSM 24194(T) = CCM 7794(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 62(Pt 6):1326-30. · 2.11 Impact Factor
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ABSTRACT: A gram-negative, rod-shaped, non-spore-forming bacterium, isolated from placental tissue of a cow, was investigated for its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain UK34/07-5(T) was shown to belong to the class Alphaproteobacteria, closely related to the type strain of Camelimonas lactis (96.0 % sequence similarity). The polyamine pattern showed the major compound spermidine and moderate amounts of putrescine. The major quinone was ubiquinone Q-10. The polar lipid profile was composed of the major compounds phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine and moderate amounts of diphosphatidylglycerol, three unidentified aminolipids and an unidentified phospholipid. The profile of major fatty acids, consisting of C(19 : 0) cyclo ω8c and C(18 : 1)ω7c, with C(18 : 0) 3-OH as the hydroxylated fatty acid, was very similar to that of C. lactis M 2040(T). The results of DNA-DNA hybridization and physiological and biochemical tests allowed both genotypic and phenotypic differentiation of the isolate from C. lactis. The relatively low 16S rRNA gene sequence similarity of 96.0 % to C. lactis M 2040(T) and marked differences in the polar lipid profiles as well as the results of physiological tests and the DNA-DNA hybridization data support the creation of a novel species, for which the name Camelimonas abortus sp. nov. is proposed, with the type strain UK34/07-5(T) ( = CIP 110303(T) = CCUG 61094(T) = DSM 24741(T) = CCM 7941(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 62(Pt 5):1117-20. · 2.11 Impact Factor
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ABSTRACT: A Gram-reaction-positive, rod-shaped bacterium, designated strain CC-VM-Y(T), was isolated from the faeces of the pill millipede Arthrosphaera magna Attems from India and was subsequently studied to determine its taxonomic position. Based on16S rRNA gene sequence similarities, the isolate clearly grouped with members of the genus Microbacterium. On the basis of pairwise comparisons of the 16S rRNA gene sequences, strain CC-VM-Y(T) was most closely related to Microbacterium insulae DS-66(T) (98 %), Microbacterium hydrocarbonoxydans DSM 160809(T) (97.8 %) and Microbacterium hominis NBRC 15708(T) (97.9 %). The peptidoglycan contained the amino acids ornithine (Orn), alanine (Ala), glycine (Gly), homoserine (Hsr) and glutamic acid (Glu) in an approximate molar ratio of 1.0 : 0.8 : 2.2 : 0.8 : 0.3. In addition, substantial amounts of threo-3-hydroxy glutamic acid (Hyg) were detected, which is characteristic of peptidoglycan type B2β. The acyl type of the peptidoglycan was glycolyl. The menaquinones of strain CC-VM-Y(T) were MK-13 (72 %), MK-12 (25 %) and MK-11 (3 %). The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid. The fatty acid profile comprised anteiso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0) as the major fatty acids, which was congruent with fatty acid profiles of other members of the genus Microbacterium. The results of physiological and biochemical tests as well as DNA-DNA hybridizations with the most closely related species, M. insulae, M. hydrocarbonoxydans and M. hominis, revealed clear phenotypic and genotypic differences between strain CC-VM-Y(T) and other members of the genus Microbacterium. Based on these results, strain CC-VM-Y(T) ( = DSM 22421(T) = CCM 7681(T)) represents a new species of the genus Microbacterium, for which the name Microbacterium arthrosphaerae sp. nov. is proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 61(Pt 6):1334-7. · 2.11 Impact Factor
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ABSTRACT: A pink-pigmented, facultatively methylotrophic bacterium, designated strain JT1(T), was isolated from a thallus of the liverwort Marchantia polymorpha L. and was analysed by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis placed the strain in a clade with Methylobacterium adhaesivum AR27(T), Methylobacterium fujisawaense DSM 5686(T), Methylobacterium radiotolerans JCM 2831(T) and Methylobacterium jeotgali S2R03-9(T), with which it showed sequence similarities of 97.8, 97.7, 97.2 and 97.4 %, respectively. However, levels of DNA-DNA relatedness between strain JT1(T) and these and the type strains of other closely related species were lower than 70 %. Cells of JT1(T) stained Gram-negative and were motile, rod-shaped and characterized by numerous fimbriae-like appendages on the outer surface of their wall (density up to 200 µm(-2)). Major fatty acids were C(18 : 1)ω7c and C(16 : 0). Based on the morphological, physiological and biochemical data presented, strain JT1(T) is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium marchantiae sp. nov. is proposed. The type strain is JT1(T) ( = DSM 21328(T) = CCUG 56108(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 61(Pt 4):870-6. · 2.11 Impact Factor
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ABSTRACT: A gram-negative, rod-shaped, non-spore-forming bacterium (strain CCUG 43427A(T)) was isolated from a patient suffering from endophthalmitis and its taxonomic position was studied. 16S rRNA gene sequence analysis indicated that this strain was a member of the genus Massilia. Strain CCUG 43427A(T) was most closely related to the type strains of Massilia timonae (97.4 % 16S rRNA gene sequence similarity) and Massilia aurea (97.2 %); levels of similarity to the type strains of all other recognized Massilia species were below 97.0 %. Chemotaxonomic data [Q-8 as major ubiquinone; phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as major polar lipids; and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), C(16 : 0), C(18 : 1)ω7c, C(12 : 0) and C(10 : 0) 3-OH as major fatty acids] supported the affiliation of the isolate to the genus Massilia. Levels of DNA-DNA relatedness of strain CCUG 43427A(T) with M. timonae CCUG 45783(T) and M. aurea AP13(T) were 60.6 % (reciprocal, 55.8 %) and 58.1 % (reciprocal, 34.0 %), respectively. Strain CCUG 43427A(T) could be differentiated from its closest phylogenetic neighbours based on a range of phenotypic characteristics. Strain CCUG 43427A(T) is therefore considered to represent a novel species of the genus Massilia, for which the name Massilia oculi sp. nov. is proposed. The type strain is CCUG 43427A(T) ( = CCM 7900(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2011; 62(Pt 2):364-9. · 2.11 Impact Factor
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ABSTRACT: A Gram-positive, non-spore-forming actinobacterium (Sj 10(T)) was isolated on tryptone soy agar from the air of a duck barn after filter sampling. Based on 16S rRNA gene sequence similarity studies, strain Sj 10(T) was shown to belong to the genus Leucobacter and was closely related to Leucobacter chromiireducens subsp. chromiireducens L-1(T) (97.8 %), Leucobacter tardus DSM 19811(T) (97.3 %) and Leucobacter luti RF6(T) (97.3 %). The peptidoglycan of strain Sj 10(T) contained 2,4-diaminobutyric acid in combination with a lower amount of lysine as diagnostic diamino acids. In addition, threonine, glycine, alanine and glutamic acid were found. Menaquinone MK-11 was the major respiratory quinone; MK-12 and MK-10 were detected in minor amounts. The polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol and one unknown component each of a phospholipid, glycolipid and aminoglycolipid. Strain Sj 10(T) contained the major fatty acids anteiso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0), like other members of the genus Leucobacter. Results of DNA-DNA hybridization, physiological and biochemical tests enabled strain Sj 10(T) to be differentiated genotypically and phenotypically from the most closely related Leucobacter species. Strain Sj 10(T) represents a novel species of the genus Leucobacter, for which the name Leucobacter aerolatus sp. nov. is proposed, with Sj 10(T) (=DSM 22806(T) =CCM 7705(T)) as the type strain.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2010; 60(Pt 12):2838-42. · 2.11 Impact Factor
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ABSTRACT: A Gram-positive-staining, non-endospore-forming actinobacterium, designated C7(T), was isolated from the leaf surface of Trifolium repens. On the basis of 16S rRNA gene sequence analysis, strain C7(T) was shown to belong to the genus Williamsia and was most closely related to Williamsia maris SJS0289/JS1(T) (98.0 % 16S rRNA gene sequence similarity), Williamsia deligens IMMIB RIV-956(T) (96.4 %) and Williamsia serinedens IMMIB SR-4(T) (95.7 %). The quinone system consisted predominantly of the menaquinones MK-9(H(2)), MK-8(H(2)) and MK-7(H(2)). The major components in the polar lipid profile were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C(16 : 1)ω7c, C(16 : 0), C(18 : 0), C(18 : 1)ω9c and tuberculostearic acid, supported the affiliation of strain C7(T) with the genus Williamsia. Physiological and biochemical analysis revealed clear differences between strain C7(T) and its closest phylogenetic neighbours. Therefore, strain C7(T) represents a novel species, for which the name Williamsia phyllosphaerae sp. nov. is proposed. The type strain is C7(T) ( = CCUG 60465(T) = CCM 7855(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2010; 61(Pt 11):2702-5. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-positive, endospore-forming rod, designated CCUG 53201(T), was isolated from a human blood sample of a 75-year-old woman. 16S rRNA gene sequence-based phylogenetic analysis showed that strain CCUG 53201(T) clustered with the type strains of species of the genus Ornithinibacillus. Strain CCUG 53201(T) was most closely related to Ornithinibacillus bavariensis WSBC 24001(T) and Ornithinibacillus californiensis DSM 16628(T) (97.9 and 98.7 % 16S rRNA gene sequence similarity, respectively). Strain CCUG 53201(T) contained a peptidoglycan of type A4β l-Orn-d-Asp. The quinone system was composed of the menaquinone MK-7 and small amounts of MK-6. The polar lipid profile of strain CCUG 53201(T) consisted of major amounts of diphosphatidylglycerol and an unidentified phospholipid, moderate amounts of phosphatidylglycerol and another two unidentified phospholipids and minor amounts of several other components. The fatty acid profile comprised mainly anteiso- and iso-branched fatty acids and was in accordance with those of members of the genus Ornithinibacillus. The polyamine pattern exhibited the major compounds spermidine and spermine. The results of physiological and biochemical tests and DNA-DNA hybridization allowed the phenotypic and genotypic differentiation of strain CCUG 53201(T) from its closest phylogenetic neighbours. We propose a novel species with the name Ornithinibacillus contaminans sp. nov., with type strain CCUG 53201(T) (=DSM 22953(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2010; 60(Pt 12):2930-4. · 2.11 Impact Factor
