Peter Kämpfer

Justus-Liebig-Universität Gießen, Gieben, Hesse, Germany

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Publications (400)1029.29 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A yellow pigmented bacterial nitrogen-fixing strain NXU-44T, isolated from the rhizosphere of switchgrass (Panicum virgatum) in Auburn, Alabama U.S.A. was studied for its taxonomic position. Cells of the isolate were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence with the sequences of the type strains of most closely related species showed that the strain belongs to the genus Flavobacterium showing the highest sequence similarities to the type strains of Flavobacterium ginsengidismutans (97.9%), Flavobacterium phragmitis (97.6%) and Flavobacterium anhuiense (97.5%). The 16S rRNA gene sequence similarities to all other Flavobacterium species were below 97.5%. The fatty acid profile of strain NXU-44T consisted of the major fatty acids C15:0 iso, C15:0 iso 2-OH/ C16:1 ω7c and C17:0 iso 3-OH. Major compounds in the polar lipid profile were phosphatidylethanolamine, phosphatidylserine, one aminolipid and two polar lipids. The quinone system was exclusively composed of menaquinone MK-6. The polyamine pattern contained the major compound sym-homospermidine and only minor amounts of other polyamines. The diagnostic diamino acid of the peptidoglycan was meso-diamopimelic acid. These data and the differentiating biochemical and chemotaxonomic properties showed, that the isolate NXU-44T represents a novel species of the genus Flavobacterium, for which we propose the name Flavobacterium nitrogenifigens sp. nov. (type strain NXU-44T = LMG 28694T = CIP 110894T).
    International Journal of Systematic and Evolutionary Microbiology 05/2015; DOI:10.1099/ijs.0.000330 · 2.80 Impact Factor
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    ABSTRACT: A gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1396T) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (Gossypium hirsutum, cultivar 'DES-119') grown at the Plant Breeding Unit facility at the E.V. Smith Research Center in Tallassee (Macon county), Alabama USA. 16S rRNA gene sequence analysis of strain JM-1396T showed high sequence similarity values to the type strains of Novosphingobium mathurense, Novosphingobium panipatense (both 98.6%), and Novosphingobium barchaimii (98.5%); sequence similarities to all other Novosphingobium species type strains were below 98.3%. DNA-DNA pairing experiments of the DNA of strain JM-1396T and N. mathurense SM117T, N. panipatense SM16T and N. barchaimii DSM 25411T showed low similarity values of 8% (reciprocal: 7%), 24% (reciprocal 26%), and 19% (reciprocal 25%), respectively. Ubiquinone Q-10 was detected as the dominant quinone; the fatty acids C18:1 7c (71.0%) and the typical 2-hydroxy fatty acid, C14:0 2-OH (11.7%), were detected as typical components. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatedylethanolamine, sphingoglycolipid and phosphatidylcholine. The polyamine pattern contained the major compound spermidine and only minor amounts of other polyamines. All these data revealed that strain JM-1396T represents a new species of the genus Novosphingobium. For this reason we propose the name Novosphingobium gossypii sp. nov. with the type strain JM-1396T (= LMG 28605T = CCM 8569T = CIP 110884T).
    International Journal of Systematic and Evolutionary Microbiology 05/2015; DOI:10.1099/ijs.0.000339 · 2.80 Impact Factor
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    ABSTRACT: A yellow pigmented bacterial isolate obtained from the healthy internal stem tissue of 1-month-old corn (Zea mays, cultivar 'Sweet Belle') grown at the Plant Breeding Unit facility at the E.V. Smith Research Center in Tallassee (Elmore county), Alabama USA (strain JM-791T), was taxonomically characterized. A polyphasic study, including 16S RNA gene sequence analysis, physiological characterisation, estimation of the ubiquinone and polar lipid patterns, and fatty acid composition revealed that strains JM-791T was similar on the basis of 16S rRNA gene sequence similarities to Sphingomonas paucimobilis (98.3%), Sphingomonas pseudosanguinis (97.5%) and Sphingomonas yabuuchiae (97.4%), but showed also pronounced differences, both genotypically and phenotypically. On the basis of these results, a novel Sphingomonas species is described, for which we propose the name Sphingomonas zeae sp. nov. with the type strain JM-791T (= LMG 28739T, = CCM 8596T).
    International Journal of Systematic and Evolutionary Microbiology 05/2015; DOI:10.1099/ijs.0.000298 · 2.80 Impact Factor
  • Stefanie P. Glaeser, Peter Kämpfer
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    ABSTRACT: To obtain a higher resolution of the phylogenetic relationships of species within a genus or genera within a family, multilocus sequence analysis (MLSA) is currently a widely used method. In MLSA studies, partial sequences of genes coding for proteins with conserved functions ('housekeeping genes') are used to generate phylogenetic trees and subsequently deduce phylogenies. However, MLSA is not only suggested as a phylogenetic tool to support and clarify the resolution of bacterial species with a higher resolution, as in 16S rRNA gene-based studies, but has also been discussed as a replacement for DNA-DNA hybridization (DDH) in species delineation. Nevertheless, despite the fact that MLSA has become an accepted and widely used method in prokaryotic taxonomy, no common generally accepted recommendations have been devised to date for either the whole area of microbial taxonomy or for taxa-specific applications of individual MLSA schemes. The different ways MLSA is performed can vary greatly for the selection of genes, their number, and the calculation method used when comparing the sequences obtained. Here, we provide an overview of the historical development of MLSA and critically review its current application in prokaryotic taxonomy by highlighting the advantages and disadvantages of the method's numerous variations. This provides a perspective for its future use in forthcoming genome-based genotypic taxonomic analyses. Copyright © 2015 Elsevier GmbH. All rights reserved.
    Systematic and Applied Microbiology 04/2015; 38(4). DOI:10.1016/j.syapm.2015.03.007 · 3.31 Impact Factor
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    ABSTRACT: An orange pigmented gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13T was isolated from the brood pouch of a diseased seahorse male of the species Hippocampus barbouri from the animal facility of the University of Giessen, Giessen, Hesse, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13T into the monophyletic cluster of the genus Mesonia within the Flavobacteriaceae. However, the strain shared only 92.2-93.8% sequence similarity to type strains of Mesonia species, with highest sequence similarity to the type strain of Mesonia aquimarina. The fatty acids profile showed a Mesonia-typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C15:0 (40.9%) followed by iso-C17:0 3-OH (14.8%). In the polyamine pattern sym-homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contain exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate-group, an amino-group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13T was 30 mol% Based on genotypic, chemotaxonomic and physiological characterizations we proposed a new species of the genus Mesonia, Mesonia hippocampi with strain 96_Hippo_TS_3/13T (= DSM 29615T = CIP-110839T) as type strain.
    International Journal of Systematic and Evolutionary Microbiology 04/2015; DOI:10.1099/ijs.0.000245 · 2.80 Impact Factor
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    ABSTRACT: A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase- negative, non-spore-forming, non-motile bacterium (131000547T) was isolated from the lungs of a cat with pneumonia. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the genus Streptobacillus with 97.6% sequence similarity to Streptobacillus moniliformis and 94.6% sequence similarity to Streptobacillus hongkongensis, respectively. The clear differentiation of strain 131000547T from Streptobacillus moniliformis and S. hongkongensis was also supported by gyrB, groEL, and recA nucleotide and amino acid sequence analysis, respectively. DNA-DNA hybridization demonstrated ≤ 19.9% (reciprocal 28.7%) DNA-DNA relatedness between strain 131000547T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain 131000547T to the family Leptotrichiaceae. Strain 131000547T has a unique profile of enzyme activities allowing a differentiation from the most closely related species. Within the genus Streptobacillus, isolate 131000547T could also unambiguously be separated from Streptobacillus moniliformis and Streptobacillus hongkongensis by both, MALDI-TOF MS and Fourier transform-infrared spectroscopy. On the basis of these data we propose the novel species Streptobacillus felis sp. nov. with the type strain 131000547T (= DSM 29248T = CCUG 66203T = CCM 8542T). Emended descriptions of the genus Streptobacillus and the species Streptobacillus moniliformis are also given.
    International Journal of Systematic and Evolutionary Microbiology 04/2015; DOI:10.1099/ijs.0.000238 · 2.80 Impact Factor
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    ABSTRACT: A yellow pigmented bacterial strain 91A-612T, isolated from the geocarposphere (soil around the peanut) of very immature peanuts (Arachis hypogaea) in Alabama, U.S.A. was studied for its taxonomic position. Cells of the isolate were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence with the sequences of the type strains of most closely related species showed that the strain belongs to the genus Chryseobacterium showing the highest sequence similarities to the type strains of Chryseobacterium molle (98.4%), Chryseobacterium pallidum (98.3%), and Chryseobacterium hominis (97.8%). The 16S rRNA gene sequence similarities to all other Chryseobacterium species were below 97.0%. The fatty acid profile of strain 91A-612T consisted of the major fatty acids C15:0 iso, C15:0 iso 2-OH/ C16:1 ω7c and C17:0 iso 3-OH. Major compounds in the polar lipid profile were phosphatidylethanolamine, and several unidentified lipids including two lipids not containing a sugar moiety, an amino group or a phosphate group (L3, L8), and an aminolipid (AL1). The quinone system was mainly composed of MK-6. The polyamine pattern contained sym-homospermidine as the major compound and moderate amounts of spermidine and spermine. DNA-DNA hybridizations between type strains of C. molle, C. pallidum and C. hominis resulted in similarity values clearly below 70%. These data and the differentiating biochemical and chemotaxonomic properties showed, that the isolate 91A-612T represents a novel species of the genus Chryseobacterium, for which we propose the name Chryseobacterium arachidiradicis sp. nov. (type strain 91A-612T = LMG 27814T = CCM 8490T= CIP 110647T).
    International Journal of Systematic and Evolutionary Microbiology 04/2015; DOI:10.1099/ijs.0.000237 · 2.80 Impact Factor
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    ABSTRACT: A slightly yellow pigmented bacterial strain (JM-87T), isolated from the stem of healthy 10-day-old sweet corn (Zea mays) was studied for its taxonomic allocation. The isolate revealed rod shaped cells, which showed a Gram-negative staining behavior. A comparison of the 16S rRNA gene sequence of the isolate showed 99.1, 97.8, and 97.4% similarity to the 16S rRNA gene sequences of the type strains of Elizabethkingia anophelis, Elizabethkingia meningoseptica, and Elizabethkingia miricola, respectively. The fatty acid profile of strain JM-87T consisted mainly of the major fatty acids C15:0 iso, C17:0 iso 3-OH, and C15:0 iso 2-OH / C16:1 ω7c/t. The quinone system of strain JM-87T contained exclusively menaquinone MK-6. The major polyamine was sym-homospermidine. The polar lipid profile consisted of the major lipid phosphatidylethanolamine and several unidentified aminolipids and other unidentified lipids. DNA-DNA hybridization experiments with E. meningoseptica CCUG 214T (=ATCC 13253T), E. miricola KCTC 12492T (=GTC 862T) and E. anophelis R26T resulted in similarities of 17% (reciprocal 16%), 30% (reciprocal 19%), and 51% (reciprocal 54%), respectively. The DNA-DNA hybridization results in addition to some differentiating biochemical properties clearly indicate, that strain JM-87T is a member of a novel species, for which the name Elizabethkingia endophytica sp. nov. is proposed. The type strain is JM-87T (= CIP 110885T = LMG 28604T = CCM 8570T).
    International Journal of Systematic and Evolutionary Microbiology 04/2015; DOI:10.1099/ijs.0.000236 · 2.80 Impact Factor
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    ABSTRACT: An intense yellow pigmented strain (SUR2(T)) isolated from dehydrated activated sludge was studied in detail to clarify its taxonomic assignment. Cells of the isolate showed a rod-shaped morphology and stained Gram-negative. Comparative 16S rRNA gene sequence analysis revealed highest similarities to the type strains of Chryseobacterium polytrichastri YG4-6(T) (98.6 %), Chryseobacterium aahli T68F(T) (97.9 %), Chryseobacterium daeguense K105(T) and Chryseobacterium gregarium DSM 79109(T) (both 97.4 %). 16S rRNA gene-sequence similarities to all other Chryseobacterium species were below 97.3 %. The fatty acid analysis of strain SUR2(T) revealed a Chryseobacterium typical profile composed mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA-DNA hybridizations with the type strains of C. polytrichastri, C. aahli, C. daeguense and C. gregarium resulted in values below 70 %. Differentiating biochemical and chemotaxonomic properties showed differences to the most closely related species and suggest that the isolate SUR2(T) represents a novel species, for which the name Chryseobacterium limigenitum sp. nov. (type strain SUR2(T) = ZIM B1019(T) = CCM 8594(T) = LMG 28734(T)) is proposed.
    Antonie van Leeuwenhoek 03/2015; 107(6). DOI:10.1007/s10482-015-0434-2 · 2.14 Impact Factor
  • PLoS ONE 03/2015; 10(3):e0119791. DOI:10.1371/journal.pone.0119791 · 3.53 Impact Factor
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    ABSTRACT: Two Gram-negative non endospore-forming rod-shaped bacteria, CC-MHSW-5T and A1392, were isolated from water of coastal hot springs located in Taiwan and China, respectively, and investigated for their taxonomic position. Both strains shared identical 16S rRNA gene sequences, a DNA DNA hybridization values > 80% and similar genomic DNA G+C values (64.3 and 64.6 mol%), but showed different genomic fingerprint pattern generated by a BOX-PCR and three RAPD-PCRs. The strains shared highest 16S rRNA gene sequence similarities to type strains of Chelativorans multitrophicus (96.7 and 96.1%), Thermovum composti (96.2 and 96.1%) and Chelativorans oligotrophicus (96.1 and 95.8%). Phylogenetic trees (based on 16S rRNA and recA gene sequence comparisons) showed a distinct clustering of both strains with the type strains of Chelativorans species and T. composti Nis3T. The quinone systems of CC-MHSW-5T and Nis3T, contained ubiquinone Q-10 as the major quinone. The major polyamines in both strains were sym-homospermidine and spermidine. Putrescine and for CC-MHSW-5T also spermidine were found in minor concentrations. Their polar lipid profile consisted of phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol. The fatty acid profile contained major amounts C18:1ω7c and C19:0 cycloω8c. On the basis of these results, a novel species is proposed based on two strains with strain CC-MHSW-5T (= CCM 8543T= LMG 28482T = DSM 29391T = CIP 110825T) as type strain, with the name Chelativorans intermedius sp. nov. Because of the relationship both in genotypic and phenotypic characters it is proposed to reclassify T. composti to Chelativorans composti comb. nov.
    International Journal of Systematic and Evolutionary Microbiology 02/2015; DOI:10.1099/ijs.0.000155 · 2.80 Impact Factor
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    ABSTRACT: A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the trachea of a white stork in Poland, designated strain 136/3T, was subjected to a comprehensive taxonomic investigation. A comparative analysis of the 16S rRNA gene sequence showed highest similarities to Corynebacterium mustelae, Corynebacterium pseudotuberculosis, Corynebacterium vitaeruminis and Corynebacterium ulcerans (96.0-96.3%). The quinone system consisted of major amounts of MK-8(H2), minor amounts of MK-9(H2) and traces of MK-8 and MK-9. The polar lipid profile of strain 136/3T contained phosphatidylinositol (PI) and phosphatidylinositol-mannoside (PIM) as major lipids and phosphatidylglycerol (PG) and an acidic glycolipid (aGL) in moderate amounts. In addition small amounts of diphosphatidylglycerol (DPG), a phosholipid (PL), an aminolipid (AL1) and two lipids of unknown group affiliation (L1 and L2) were found. The polyamine pattern was composed of the major components spermidine and spermine. 1,3-diaminopropane, putrescine, cadaverine, sym-homospermidine, and tyramine were found in minor or trace amounts. The diaminoacid of the peptidoglycan was meso-diaminopimelic acid. In the fatty acid profile straight chain, saturated and mono-unsaturated fatty acids predominated (C18:1ω9c, C16:1ω7c, C16:0). Corynemycolic acids were detected. Physiological traits as well as unique traits in the polar lipid profile and the fatty acid pattern distinguished strain 136/3T from the next related species. All these data point to the fact, that strain 136/3T is representing a novel species of the genus Corynebacterium for which we propose the name Corynebacterium pelargi sp. nov.. The type strain is 136/3T (= CIP 110778T = CCM 8517T = LMG 28174T).
    International Journal of Systematic and Evolutionary Microbiology 02/2015; DOI:10.1099/ijs.0.000115 · 2.80 Impact Factor
  • Peter Kämpfer, Stefanie P Glaeser
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    ABSTRACT: Serratia glossinae DSM 22080T was compared with Serratia fonticola ATCC 29844T to clarify the taxonomic relationship of both species. 16S rRNA gene sequence comparisons demonstrated that these species share 99.6% sequence similarity. Investigation of substrate utilization profiles displayed no striking differences to the type strains of both species. DNA-DNA hybridizations between both strains showed a 100% (99.9%) similarity. Therefore, the reclassification of Serratia glossinae as Serratia fonticola is proposed, based upon the estimated phylogenetic position derived from 16S rRNA gene sequence data, biochemical data, and DNA-DNA hybridization results.
    International Journal of Systematic and Evolutionary Microbiology 02/2015; DOI:10.1099/ijs.0.000112 · 2.80 Impact Factor
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    ABSTRACT: A novel, Gram-staining-negative, rod-shaped, aerobic and motile bacterium, designated strain CC-SKC2T, was isolated from the root tumor of a green bell pepper (Capsicum annuum var. grossum) plant in Taiwan. Cells were positive for oxidase and catalase activities and exhibited growth at 25–37 °C, pH 4.0–9.0 and tolerated NaCl concentrations up to 4.0 % (w/v). Strain CC-SKC2T is able to trigger nodulation in soybean (Glycine max Merr.), but not in Capsicum annuum var. grossum, red bean (Vigna angularis), sesbania (Sesbania roxburghii Merr.) or alfalfa (Medicago varia Martin.). The novel strain shared highest 16S rRNA gene sequence similarity to Rhizobium rhizoryzae KCTC 23652T and Rhizobium straminoryzae CC-LY845T (both 97.5 %) followed by Rhizobium lemnae L6-16T (97.3 %), Rhizobium pseudoryzae KCTC 23294T (97.1 %), and Rhizobium paknamense NBRC 109338T (97.0 %), whereas other Rhizobium species shared T with R. rhizoryzae KCTC 23652T, R. pseudoryzae KCTC 23294T and R. paknamense NBRC 109338T were 11.4, 17.2 and 17.0 %, respectively (reciprocal values were 11.1, 28.3 and 24.0 %, respectively). Phylogenetic analysis based on 16S rRNA, atpD and recA genes revealed a distinct taxonomic position attained by strain CC-SKC2T with respect to other Rhizobium species. The major fatty acids in strain CC-SKC2T were C16:0, C19:0 cyclo ω8c, C14:0 3-OH and/or C16:1 iso I and C18:1ω7c and/or C18:1ω6c. The polyamine pattern showed predominance of spermidine and moderate amounts of sym-homospermidine. The predominant quinone system was ubiquinone (Q-10) and the DNA G+C content was 60.5 mol%. On the basis of polyphasic taxonomic evidence presented here, strain CC-SKC2T is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium capsici sp. nov. is proposed. The type strain is CC-SKC2T (=BCRC 80699T = JCM 19535T).
    Antonie van Leeuwenhoek 01/2015; 107(3). DOI:10.1007/s10482-014-0371-5 · 2.14 Impact Factor
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    ABSTRACT: A Gram-negative, rod-shaped and motile bacterial isolate, designated strain NS9T, isolated from air of the Sainsbury Centre For Visual Arts in Norwich, England was subject to a polyphasic taxonomic study including phylogenetic analyses based on partial 16S rRNA, gyrB and lepA gene sequences and phenotypic characterisation. The 16S rRNA gene sequence of NS9T identified Massilia haematophila CCUG 38318T, Massilia niastensis 5516S-1T (both 97.7 % similarity), Massilia aerilata 5516S-11T and Massilia tieshanensis TS3T (both 97.4 % similarity) as the next related species. In partial gyrB and lepA sequences NS9T shared highest similarities with M. haematophila CCUG 38318T (94.5 %) and M. aerilata 5516-11T (94.3 %), respectively. These sequence data are demonstrating affiliation of NS9T to the genus Massilia. The detection of the predominant ubiquinone Q-8, a polar lipid profile consisting of the major compounds diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and a polyamine pattern containing 2-hydroxyputrescine and putrescine were in agreement with assignment of strain NS9T to the genus Massilia. Major fatty acids are summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C16:0, C18:1ω7c and C10:0 3-OH. Dissimilarities in partial lepA and gyrB gene sequences as well as results from DNA-DNA hybridizations demonstrate that strain NS9T is a representative of a so far not described species of the genus Massilia which is also distinguished from its close relatives based on physiological and biochemical traits. Hence, we are here describing a novel species for which we are proposing the name Massilia norwichensis sp. nov. with the type strain NS9T= CCUG 65457T = LMG 28164T.
    International Journal of Systematic and Evolutionary Microbiology 01/2015; DOI:10.1099/ijs.0.068296-0 · 2.80 Impact Factor
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    ABSTRACT: A slightly yellow pigmented strain (JM-534T) isolated from the rhizoplane of a field-grown Zea mays plant was investigated using a polyphasic approach for its taxonomic allocation. Cells of the isolate were observed to be rod-shaped and to stain Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to Chryseobacterium lactis (98.9 %), Chryseobacterium joostei and Chryseobacterium indologenes (both 98.7 %), and Chryseobacterium viscerum (98.6 %). Sequence similarities to all other Chryseobacterium species were 98.5 % or below. The fatty acid analysis of the strain resulted in a Chryseobacterium typical pattern consisting mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA–DNA hybridizations with the type strains of C. lactis, C. joostei, C. viscerum and C. indologenes resulted in values below 70 %. Genomic fingerprinting showed that the isolate was very different to the type strains of these species. Differentiating biochemical and chemotaxonomic properties showed that the isolate JM-534T represents a novel species, for which the name Chryseobacterium rhizoplanae sp. nov. (type strain JM-534T = LMG 28481T = CCM 8544T = CIP 110828T) is proposed.
    Antonie van Leeuwenhoek 12/2014; 107(2). DOI:10.1007/s10482-014-0349-3 · 2.14 Impact Factor
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    ABSTRACT: A Gram-positive staining, aerobic organism, isolated from the the Red Sea sponge Spheciospongia vagabunda was investigated for its taxonomic position. Based on 16S rRNA gene sequence analysis strain EG49T was most closely related to Actinokineospora cibodasensis and Actinokineospora baliensis (both 97.3%) and Actinokineospora diospyrosas and Actinokineospora auranticolor (both 97.0%). The 16S rRNA gene sequence similarity to all other Actinokineospora species was < 97.0 %. The quinone system of strain EG49T contains the menaqinone MK-9(H4), (47%), MK-9(H6), (27%) and MK-9(H2), (15%) in major amounts. Minor amounts of MK-7(H4), (2%), MK-9(H0), (1%), MK-9(H8) (3%) and MK-10(H4) (3%) were detected as well in addition to MK-8(H4), MK-8(H6), MK-10(H2) and MK-10(H6) (all <1%). The diagnostic diaminoacid of the peptidoglycan is meso-diaminopimelic acid. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, and hydroxyl-phosphatidylethanolamine are predominant. Phosphatidylinositol-mannoside, two unidentified phospholipids and two glycoglipids as well as one aminoglycolipid, one aminolipid and one unidentified lipid are found in addition. Fatty acid profile composed of mainly iso-branched fatty acids: iso-C16:0, iso-C14:0, iso-C15:0, and iso-C16:1 H. All these data clearly supported the allocation of the strain to the genus Actinokineospora. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain EG49T from the most closely related Actinokineospora species. Strain EG49T represents a new species of the genus Actinokineospora, for which we propose the name Actinokineospora spheciospongiae sp. nov., with strain EG49T (= DSM 45935T = CCM 8480T = LMG 27700T) as the type strain.
    International Journal of Systematic and Evolutionary Microbiology 12/2014; 65(Pt 3). DOI:10.1099/ijs.0.000031 · 2.80 Impact Factor
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    ABSTRACT: Five beige pigmented bacterial strains (176/10T, 178/10, 182/10, 185/7 and 193/8), showing 99.9% 16S rRNA gene sequence similarity to each other and isolated from white storks in Poland were investigated in a polyphasic taxonomic study. The cells of all these isolates were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequences of these organisms with the sequences of the type strains of most closely related Psychrobacter species showed the highest sequence similarities to Psychrobacter pulmonis and Psychrobacter faecalis (both 97.1%). The 16S rRNA gene sequence similarities to all other Psychrobacter species were below 96.3%. All 5 isolates showed an identical profile of physiological reactions and almost identical fatty acid profiles consisting of mainly C18:1 ω9c, but showing also 3-OH 12:0 as characteristic hydroxylated fatty acid. A quinone system with mainly ubiquinone Q-8 was detected and the polar lipid profile of the type strain 176/10T was mainly composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and diphosphatidylglycerol, beside some hitherto uncharacterized phospholipids and one aminolipid. Major polyamines were spermidine and putrescine. DNA-DNA hybridizations between the type strain 176/10T and the type strains of P. pulmonis and P. faecalis resulted in similarity values clearly below 70%. These results indicate, that the strains represent a novel species, for which the name Psychrobacter ciconiae sp. nov. (type strain 176/10T = CIP 110777T = LMG 28175T = CCM 8519T) is proposed.
    International Journal of Systematic and Evolutionary Microbiology 12/2014; 65(Pt 3). DOI:10.1099/ijs.0.000013 · 2.80 Impact Factor

Publication Stats

8k Citations
1,029.29 Total Impact Points

Institutions

  • 1995–2015
    • Justus-Liebig-Universität Gießen
      • Institute of Applied Microbiology
      Gieben, Hesse, Germany
    • RWTH Aachen University
      • Institute of Hygiene and Environmental Medicine
      Aachen, North Rhine-Westphalia, Germany
  • 2013
    • Helmholtz Centre for Infection Research
      Brunswyck, Lower Saxony, Germany
  • 2005–2013
    • University of Bonn
      • • Institute of Nutrition and Food Science (IEL)
      • • Institute for Hygiene and Public Health
      Bonn, North Rhine-Westphalia, Germany
  • 2011
    • Universität Kassel
      • Institute of Biology
      Kassel, Hesse, Germany
  • 2010–2011
    • Bundesanstalt für Arbeitsschutz und Arbeitsmedizin
      Dortmund, North Rhine-Westphalia, Germany
  • 2002–2011
    • University of Veterinary Medicine in Vienna
      • Institute of Bacteriology, Mycology and Hygiene
      Wien, Vienna, Austria
  • 2009
    • Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
      Jena, Thuringia, Germany
  • 2008–2009
    • National Chung Hsing University
      • Department of Soil and Environmental Sciences
      Taichung, Taiwan, Taiwan
    • University of Bristol
      Bristol, England, United Kingdom
  • 2004–2009
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2007
    • Royal Perth Hospital
      Perth City, Western Australia, Australia
  • 2000–2007
    • Universität Stuttgart
      • Institute of Organic Chemistry
      Stuttgart, Baden-Württemberg, Germany
  • 2002–2006
    • University of Vienna
      Wien, Vienna, Austria
  • 2003
    • University of Helsinki
      Helsinki, Uusimaa, Finland
  • 1995–2002
    • Universität Ulm
      Ulm, Baden-Württemberg, Germany
  • 1998
    • Centers for Disease Control and Prevention
      • National Center for Emerging and Zoonotic Infectious Diseases
      Druid Hills, GA, United States
  • 1988–1996
    • Technische Universität Berlin
      Berlín, Berlin, Germany
  • 1992
    • Freie Universität Berlin
      Berlín, Berlin, Germany
  • 1991
    • Hochschuelerschaft an der Technischen Universitaet Graz
      Berlin, New Hampshire, United States