Peter Kämpfer

Justus-Liebig-Universität Gießen, Gieben, Hesse, Germany

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Publications (369)784.02 Total impact

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    ABSTRACT: A slightly yellow pigmented strain (JM-534T) isolated from the rhizoplane of a field-grown Zea mays plant was investigated using a polyphasic approach for its taxonomic allocation. Cells of the isolate were observed to be rod-shaped and to stain Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to Chryseobacterium lactis (98.9 %), Chryseobacterium joostei and Chryseobacterium indologenes (both 98.7 %), and Chryseobacterium viscerum (98.6 %). Sequence similarities to all other Chryseobacterium species were 98.5 % or below. The fatty acid analysis of the strain resulted in a Chryseobacterium typical pattern consisting mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA–DNA hybridizations with the type strains of C. lactis, C. joostei, C. viscerum and C. indologenes resulted in values below 70 %. Genomic fingerprinting showed that the isolate was very different to the type strains of these species. Differentiating biochemical and chemotaxonomic properties showed that the isolate JM-534T represents a novel species, for which the name Chryseobacterium rhizoplanae sp. nov. (type strain JM-534T = LMG 28481T = CCM 8544T = CIP 110828T) is proposed.
    Antonie van Leeuwenhoek 12/2014; · 2.07 Impact Factor
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    ABSTRACT: A Gram-positive staining, aerobic organism, isolated from the the Red Sea sponge Spheciospongia vagabunda was investigated for its taxonomic position. Based on 16S rRNA gene sequence analysis strain EG49T was most closely related to Actinokineospora cibodasensis and Actinokineospora baliensis (both 97.3%) and Actinokineospora diospyrosas and Actinokineospora auranticolor (both 97.0%). The 16S rRNA gene sequence similarity to all other Actinokineospora species was < 97.0 %. The quinone system of strain EG49T contains the menaqinone MK-9(H4), (47%), MK-9(H6), (27%) and MK-9(H2), (15%) in major amounts. Minor amounts of MK-7(H4), (2%), MK-9(H0), (1%), MK-9(H8) (3%) and MK-10(H4) (3%) were detected as well in addition to MK-8(H4), MK-8(H6), MK-10(H2) and MK-10(H6) (all <1%). The diagnostic diaminoacid of the peptidoglycan is meso-diaminopimelic acid. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, and hydroxyl-phosphatidylethanolamine are predominant. Phosphatidylinositol-mannoside, two unidentified phospholipids and two glycoglipids as well as one aminoglycolipid, one aminolipid and one unidentified lipid are found in addition. Fatty acid profile composed of mainly iso-branched fatty acids: iso-C16:0, iso-C14:0, iso-C15:0, and iso-C16:1 H. All these data clearly supported the allocation of the strain to the genus Actinokineospora. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain EG49T from the most closely related Actinokineospora species. Strain EG49T represents a new species of the genus Actinokineospora, for which we propose the name Actinokineospora spheciospongiae sp. nov., with strain EG49T (= DSM 45935T = CCM 8480T = LMG 27700T) as the type strain.
    International journal of systematic and evolutionary microbiology. 12/2014;
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    ABSTRACT: Five beige pigmented bacterial strains (176/10T, 178/10, 182/10, 185/7 and 193/8), showing 99.9% 16S rRNA gene sequence similarity to each other and isolated from white storks in Poland were investigated in a polyphasic taxonomic study. The cells of all these isolates were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequences of these organisms with the sequences of the type strains of most closely related Psychrobacter species showed the highest sequence similarities to Psychrobacter pulmonis and Psychrobacter faecalis (both 97.1%). The 16S rRNA gene sequence similarities to all other Psychrobacter species were below 96.3%. All 5 isolates showed an identical profile of physiological reactions and almost identical fatty acid profiles consisting of mainly C18:1 ω9c, but showing also 3-OH 12:0 as characteristic hydroxylated fatty acid. A quinone system with mainly ubiquinone Q-8 was detected and the polar lipid profile of the type strain 176/10T was mainly composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and diphosphatidylglycerol, beside some hitherto uncharacterized phospholipids and one aminolipid. Major polyamines were spermidine and putrescine. DNA-DNA hybridizations between the type strain 176/10T and the type strains of P. pulmonis and P. faecalis resulted in similarity values clearly below 70%. These results indicate, that the strains represent a novel species, for which the name Psychrobacter ciconiae sp. nov. (type strain 176/10T = CIP 110777T = LMG 28175T = CCM 8519T) is proposed.
    International journal of systematic and evolutionary microbiology. 12/2014;
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    ABSTRACT: A Gram-stain-positive bacterial isolate, strain 280/10T was isolated from the trachea of a white stork. The isolate was morphologically rod to coccoid-shaped, non-motile and showed no oxidase-activity. Highest 16S rRNA gene sequences similarity was found to the type strain of Corynebacterium ciconiae (97.3% similarity) as the next relative of strain 280/10T, all other 16S rRNA gene sequence similarities to Corynebacterium type strains were below 94.2% Strain 280/10T showed a quinone system consisting predominantly of menaquinone MK-8(H2) and minor amounts of MK-9(H2) with low amounts and MK-8. The diamino acid of the peptidoglycan was meso-diaminopimelic acid.The polar lipid profiles revealed the major compounds diphosphatidylglycerol, phoshatidylglycerol, phosphatidylinositol and an acidic glycolipid (aGL). Two phosphatidylinositol-mannosides and several unidentified lipids were present, as well. The strain contained corynemycolic acids. Only low amounts of cellular polyamines were detected. Spermidine and spermine were predominant in the polyamine pattern and putrescine was present in moderate amounts in 280/10T. In the fatty acid profiles predominantly C18:1ω9c, and C16:0 were detected. The strain was also distinguishable from the most closely related species C. ciconiae. As a conclusion, a novel species of the genus Corynebacterium is proposed with the name Corynebacterium trachiae sp. nov. with the type strain 280/10T (= CIP 110796T = LMG 28214T).
    International journal of systematic and evolutionary microbiology. 12/2014;
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    ABSTRACT: A cream-colored, Gram-negative, aerobic, non-motile, rod to irregular shaped bacterium, 119/4T, was isolated from a choana swab of a white stork nestling on sheep blood agar. The 16S rRNA gene sequence analysis and subsequent comparisons showed that it was placed into the family Rhodobacteraceae showing 94.9 % similarity to Gemmobacter tilapiae and 94.6 % similarity to Gemmobacter nectariphilus, but also similarly low sequence similarity to Rhodobacter viridis (94.8 %), Rhodobacter veldkampii (94.6 %) and Paenirhodobacter enshiensis (94.6%). Construction of the phylogenetic trees showed that strain 119/4T clustered close to the species of the genus Gemmobacter. The quinone system contained high amounts of ubiquinone Q-10 with traces of Q-8, Q-9 and Q-11 and the fatty acid profile consisted mainly of C18:1ω7c, C16:1 ω7c/ iso 15:0 2-OH, and 3-OH C10:0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phoshatidylglycerol and phosphatidylcholine. Major polyamines were putrescine and spermidine. On the basis of the 16S rRNA gene sequence analysis and of the chemotaxonomic and physiological data, strain 119/4T represents a new species of the genus Gemmobacter, for which the name Gemmobacter intermedius sp. nov., is proposed. The type strain is 119/4T (= CIP 110795T = LMG 28215T = CCM 8510T).
    International journal of systematic and evolutionary microbiology. 12/2014;
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    ABSTRACT: During a study assessing the diversity of Bacillus pumilus group it became apparent that the type strains of both Bacillus aerophilus and Bacillus stratosphaericus are not available from any established culture collection nor from the authors who originally described them. Therefore, type strains of these species cannot be included in any further scientific study. It is then proposed that the Judicial Commission of the International Committee of Systematics of Prokaryotes place the names Bacillus aerophilus and Bacillus stratosphaericus on the list of rejected names, if suitable replacements for type strains are not found or neotype strains are not proposed within two years following the publication of this Request for an Opinion.
    International journal of systematic and evolutionary microbiology. 12/2014;
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    ABSTRACT: Two Gram-negative, rod-shaped strains, T2.1T and W5.1.1T, isolated from the larvae of the mosquito Anopheles arabiensis were investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strains T2.1T and W5.1.1T were shown to belong to the genus Thorsellia, both with 97.8% similarity to the type strain of T. anophelis, and with 98.1% similarity to each other. Chemotaxonomic data supported the allocation of the strains to the genus Thorsellia: their major fatty acids are C18:1ω7c, C16:0, and C14:0; they harbor a ubiquinone Q-8 quinone system and a polyamine pattern with the major compound 1,3-diaminopropane. Qualitative and quantitative differences in their polar lipid profile distinguish the two strains T2.1T and W5.1.1T from each other and from T. anophelis. Average nucleotide identity (ANI), DNA-DNA hybridization, Multi Locus sequence analysis (MLSA) as well as physiological and biochemical tests allowed to distinguish both genotypically and phenotypically T2.1T and W5.1.1T from each other and from the type strain of T. anophelis. Thus, we propose that these isolates represent two novel species of the genus Thorsellia, named Thorsellia kenyensis sp. nov. and Thorsellia kandunguensis sp. nov., respectively. The type strains are T2.1T (=CCM 8545 = LMG 28483T) and W5.1.1T (= LMG 28213T =CIP 110794T), respectively. Furthermore, phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the genus Thorsellia formed a separate branch, distinct from Enterobacteriaceae, Pasteurellaceae, and Orbaceae. As a consequence, a new family Thorselliaceae fam. nov. is proposed.
    International journal of systematic and evolutionary microbiology. 11/2014;
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    ABSTRACT: A yellow pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1T), was isolated from the rhizosphere of a field-grown Zea mays plant in Auburn, Alabama, U.S.A. The 16S rRNA gene sequence analysis of strain JM-1T showed high sequence similarity to the type strains of Novosphingobium capsulatum (98.9%), Novosphingobium aromaticivorans (97.4%), Novosphingobium subterraneum (97.3%), and Novosphingobium taihuense (97.1%), sequence similarities to all other Novosphingobium species type strains were below 97.0%. DNA-DNA hybridizations of strain JM-1T and N. capsulatum DSM 30196T, N. aromaticivorans SMCC F199T and N. subterraneum SMC B0478T showed low similarity values of 33% (reciprocal: 21%), 14% (reciprocal 16%), and 36% (reciprocal 38%), respectively. As the major respiratory quinones, ubiquinone Q-10 was detected and the pre-dominant fatty acid C18:1 ω7c (71.0%), and the typical 2-hydroxy fatty acids, C14:0 2-OH (11.7%) was detected. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatedylethanolamine, sphingoglycolipid and phosphatidylcholine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, ubiquinone, polar lipid, and fatty acid composition revealed that strain JM-1T represents a new species of the genus Novosphingobium. For this reason we propose the name Novosphingobium rhizosphaerae sp. nov. with the type strain JM-1T (= LMG 28479T = CCM 8547T).
    International journal of systematic and evolutionary microbiology. 10/2014;
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    ABSTRACT: Two Gram-negative, rod-shaped, non-spore-forming bacteria, isolated from metal working fluids, were investigated for their taxonomic position. On the basis of 16S rRNA gene sequence phylogeny, both strains (MPA 1113T, and MPA 1105T) formed a distinct cluster with 97.6% sequence similarity among each other in the vicinity of members of the genera Methylobacterium, Camelimonas, Chelatococcus, Bosea, Salinarimonas and Microvirga to which they showed were low sequence similarity (below 94%, respectively). Predominant compound in the polyamine pattern of the two strains was spermidine and in the quinone system was ubiquinone Q-10. The polar lipid profiles were composed of major compounds phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, major or moderate amounts of diphosphatidylglycerol, two unidentified glycolipids and three unidentified aminolipids. Several minor lipids were detected, as well. Major fatty acids were either C19:0 cyclo ω8c or C18:1 ω7c. The results of fatty acid analysis and physiological and biochemical tests allowed both genotypic and phenotypic differentiation of both isolates from each other and chemotaxonomic traits from the most closely related genera. In summary, low 16S rRNA gene sequence similarities and markedly differences in the polar lipid profiles as well as in the polyamine patterns suggests a novel genus for which the name Pseudochelatococcus gen. nov. is proposed with the species P. lubricantis sp. nov. with the type strain MPA 1113T (= CCM 8528T = LMG 28286T) and P. contaminans sp. nov. the type strain MPA 1105T (= CCM 8527T = LMG 28285T).
    International journal of systematic and evolutionary microbiology. 10/2014;
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    ABSTRACT: A Gram-negative, rod-shaped and motile bacterial isolate, designated strain NS9T, isolated from air of the Sainsbury Centre For Visual Arts in Norwich, England was subject to a polyphasic taxonomic study including phylogenetic analyses based on partial 16S rRNA, gyrB and lepA gene sequences and phenotypic characterisation. The 16S rRNA gene sequence of NS9T identified Massilia haematophila CCUG 38318T, Massilia niastensis 5516S-1T (both 97.7 % similarity), Massilia aerilata 5516S-11T and Massilia tieshanensis TS3T (both 97.4 % similarity) as the next related species. In partial gyrB and lepA sequences NS9T shared highest similarities with M. haematophila CCUG 38318T (94.5 %) and M. aerilata 5516-11T (94.3 %), respectively. These sequence data are demonstrating affiliation of NS9T to the genus Massilia. The detection of the predominant ubiquinone Q-8, a polar lipid profile consisting of the major compounds diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and a polyamine pattern containing 2-hydroxyputrescine and putrescine were in agreement with assignment of strain NS9T to the genus Massilia. Major fatty acids are summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C16:0, C18:1ω7c and C10:0 3-OH. Dissimilarities in partial lepA and gyrB gene sequences as well as results from DNA-DNA hybridizations demonstrate that strain NS9T is a representative of a so far not described species of the genus Massilia which is also distinguished from its close relatives based on physiological and biochemical traits. Hence, we are here describing a novel species for which we are proposing the name Massilia norwichensis sp. nov. with the type strain NS9T= CCUG 65457T = LMG 28164T.
    International journal of systematic and evolutionary microbiology. 10/2014;
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    ABSTRACT: A Gram-positive, rod-shaped endospore-forming Bacillus isolate Bi.FFUP1 (T) recovered in Portugal from a health product was subjected to a polyphasic study and compared with the type strains of Bacillus pumilus, Bacillus safensis, Bacillus altitudinis and Bacillus xiamenensis, the phenotypically and genotypically most closely related species. Acid production from D-cellobiose, D-glucose and D-mannose and absence of acid production from D-arabinose, erythritol, inositol, maltose, mannitol, D-raffinose, rhamnose, sorbitol, starch and L-tryptophan discriminated this new isolate from the type strains of the most closely related species. Additionally, a significant different protein and carbohydrate signature was evidenced by spectroscopic techniques, MALDI-TOF/MS and FTIR-ATR. Using a chemometric approach, the score plot generated by principal component analysis clearly delineated the isolate as a separate cluster. The quinone system for strain Bi.FFUP1 (T) was MK-7 and major polar lipids were diphosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. Strain Bi.FFUP1 (T) showed >99% 16S rRNA gene sequence similarity with B. safensis FO-036b(T), B. pumilus (7061(T) and SAFR-032), B. altitudinis 41KF2b(T) and B. xiamenensis HYC-10(T). Differences in strain Bi.FFUP1 (T) gyrB and rpoB sequences in comparison with the most closely related species and DNA-DNA hybridization experiments with Bi.FFUP1 (T) and B. pumilus ATCC 7061(T), B. safensis FO-036b(T), B. altitudinis 41KF2b(T) and B. xiamenensis HYC-10(T) gave relatedness values of 39.6% (reciprocal 38.0%), 49.9% (reciprocal 42.9%), 61.9% (reciprocal 52.2%) and 61.7% (reciprocal 49.2%), respectively, supported the delineation of strain Bi.FFUP1 (T) as a representative of a novel species of the genus Bacillus, for which the name Bacillus invictus sp. nov. is proposed, with strain Bi.FFUP1 (T) (DSM 26896(T) and CCUG 64113(T)) as the type strain.
    International journal of systematic and evolutionary microbiology 08/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-positive staining, aerobic endospore forming bacterium (P-207T) was isolated from a rhizosphere soil sample in Auburn, Alabama, U.S.A.. On the basis of 16S rRNA gene sequence similarity comparisons, strain P-207T was grouped in the vicinity of representatives of the genera Virgibacillus, Ornithinibacillus, Cerasibacillus, Lentibacillus, and Oceanobacillus, but could not clearly be assigned to one of these genera. The highest similarity was found to the sequence of Virgibacillus carmonensis AJ316302 (94.4%), however, the 16S rRNA gene sequence similarity to the type strain of the type species of Virgibacillus, Virgibacillus pantothenticus was only 92.9%. The quinone system of strain P-207T consists of menaquinone MK-7, predominantly. The polar lipid profile exhibited the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and moderate to minor amounts of several unidentified phospholipids, glycolipids, phosphoglycolipids, an aminophospholipid and an aminolipid. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid and the polyamine pattern contained predominantly spermidine and spermine. The major fatty acids, which were C15:0 anteiso, C17:0 anteiso, C16:0 iso, and C15:0 iso. The G+C content of the genomic DNA was 34 mol%. Because of the low sequence similarity of strain P-207T to all representatives of Virgibacillus, Ornithinibacillus, Cerasibacillus, Lentibacillus, and Oceanobacillus, which was always < 95% and their unique lipid patterns, we propose that strain P-207T represents a novel species in a novel genus for which the name Pseudogracilibacillus auburnensis gen. nov. sp. nov. is proposed. The type strain is P-207T (= CCM 8509T = LMG 28212T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-positive, aerobic organisms, isolated from the healthy stem of a Zea mays plant was studied for its taxonomic position. Based on 16S rRNA gene sequence analysis strain JM-1068T was grouped into the genus Nocardioides, most closely related to Nocardioides alkalitolerans (97.1 %). The 16S rRNA gene sequence similarity to all other Nocardioides species was ≤ 96.1 %. The quinone system of strain JM-1068T contains the major menaquinone MK-8(H4). The diagnostic diaminoacid of the peptidoglycan is LL-diaminopimelic acid. In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids are predominant. The polyamine pattern contains predominantly spermidine and spermine. Fatty acid profile composed mainly of iso-branched and unsaturated fatty acids. This supported the allocation of the strain to the genus Nocardioides. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain JM-1068T from N. alkalitolerans and the most closely related species. JM-1068T represents a new species of the genus Nocardioides, for which we propose the name Nocardioides zeae sp. nov., with JM-1068T (= CIP 110696T = LMG 28079T) as the type strain.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2014; · 2.11 Impact Factor
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    ABSTRACT: ABSTRACT A novel myxobacterium MCy1366T (Ar1733) was isolated in 1981 from a soil sample collected from a region near Tokyo, Japan. It displayed general myxobacterial features like Gram negative staining, rod shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting body-like aggregates and myxospore-like structures. The strain was mesophilic, aerobic and showed chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like cephalosporin C, kanamycin, gentamycin, hygromycin B, polymyxin and bacitracin and the key fatty acids of whole cell hydrolysates were iso-C15:0, iso-C17:0, and iso-C17:0 2-OH. The genomic G+C content of the novel strain is 65.6 mol%. The 16S rRNA gene sequence showed highest similarity (97.60%) to "Stigmatella koreensis" strain KYC-1019 (not validly described taxon, GenBank accession no. EF112185). The phylogenetic analysis based on 16S rRNA gene sequences and MALDI-TOF data revealed a novel branch in the family Myxococcaceae. DNA-DNA hybridization showed only 28% similarity between the novel strain and the closest species, Corallococcus exiguus DSM 14696T (97% 16S rRNA gene sequence similarity). A recent isolate from a Switzerland soil sample and designated reference strain, MCy10622 displayed 99.9% 16S rRNA gene similarity and showed almost the same characteristics with MCy1366T. Since some morphological features like fruiting body like aggregates were barely reproducible in the type strain, the newly isolated strain MCy10622 was also intensively studied. On the basis of a comprehensive taxonomic study, we propose a novel genus and species, Aggregicoccus edonensis gen. nov., sp. nov., for strain MCy1366T and MCy10622. The type strain for the genus Aggregicoccus is MCy1366T (=DSM 27872T =NCCB 100468T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-staining-positive, aerobic, non-endospore forming organism, isolated as a seed endophyte (colonizing the internal healthy tissue of plant seed) of sweet corn (Zea mays) was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence similarity comparisons, strain CSE-5610T was grouped into the genus Cohnella, most closely related to Cohnella ginsengisoli GR21-5T (98.1 %) and Cohnella plantaginis YN-83T (97.5%). The 16S rRNA gene sequence similarity to other Cohnella species was < 96.6 %. DNA-DNA hybridization of strain CSE-5610T with Cohnella ginsengisoli DSM 18997T and Cohnella plantaginis DSM 25424T were 58% (reciprocal: 24%) and 30 (reciprocal: 27%), respectively. The fatty acid profile from whole cell hydrolysates supported the allocation of the strain to the genus Cohnella. Iso- and anteiso-branched fatty acids were found as major compounds. meso-Diaminopimelic acid was identified as cell wall diaminoacid. The quinone system consisted predominantly of menaquinone MK-7. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, a phospholipid and minor amounts of two polar lipids. In the polyamine pattern spermidine was the major polyamine. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain CSE-5610T from the two closely related species. Hence, CSE-5610T represents a new species of the genus Cohnella, for which we propose the name Cohnella rhizosphaerae sp. nov., with CSE-5610T (= LMG 28080T = CIP 110695T) as the type strain.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A yellow pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070T), was isolated as a presumptive endophyte from internal stem tissue of healthy corn stem. Phylogenetic analyses based on the 16S rRNA Gene sequence placed strain JM-1070T in the monophyletic cluster of the genus Mucilaginibacter with closest affiliation to the type strains of Mucilaginibacter composti (98%) and Mucilaginibacter calamicampi (97.2%). The 16S rRNA gene sequence similarity to all other Mucilaginibacter type strains was between 93.4 to 96.0%. DNA-DNA hybridizations of strain JM-1070T and the type strains of M. composti LMG 23497T and M. calamicampi CCUG 63418T showed low similarity values of 13% (reciprocal: 18%), and 52% (reciprocal 54.4%). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids were C15:0 iso (32.2%), C15:0 iso 2-OH/ C16:1 ω7c (measured as summed feature 3) (31.8%), and C17:0 iso 3-OH (15.1%) Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly sym-homospermidine. Characterization by 16S rRNA gene sequence, physiological parameters, polyamine, ubiquinone, polar lipid, and fatty acid composition revealed that strain JM-1070T represents a new species of the genus Mucilaginibacter. For this reason we propose the name Mucilaginibacter auburnensis sp. nov. with the type strain JM-1070T (= CIP 110694T = LMG 28078T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A gram-negative, rod-shaped, oxidase positive, non-spore-forming, non-motile bacterium (strain 280T), isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus Paenochrobactrum group with a 16S rRNA gene sequence similarity of 98.8% to the both species, Paenochrobactrum gallinarii and Paenochrobactrum glaciei, respectively. This allocation was confirmed by the fatty acid data (major fatty acids: C18:1ω7c and C19:0 cyclo ω8c), polyamine pattern with the major compound putrescine and relatively high amounts of spermidine. Also the polar lipid profile with phosphatidylethanolamine, phosphatiylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholin and the genus-specific 'stretched aminolipid'; was well in line with the description of the genus Paenochrobactrum. The quinone system consists predominantly of ubiquinone Q-10 with traces of Q9 and Q11. DNA-DNA hybridization of strain 280T with Paenochrobactrum gallinarii Sa25T and Paenochrobactrum glaciei KMM 3858T showed similarity values of 38.8% (reciprocal 20.2%) and 30.2% (reciprocal 29.8%), respectively. These results in combination with differentiating physiological and biochemical data clearly showed that strain 280T merits species status. We propose the name Paenochrobactrum pullorum sp. nov. for this strain with the type strain 280T (= LMG 28095T = CIP 110700T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: Two yellow pigmented bacterial strains (100T and C26T), showing 98.4% 16S rRNA gene sequence similarity to each other and isolated from chicken in Germany and as a contaminant from an agar plate of a rhizosphere sample were studied in a polyphasic taxonomic approach. Cells of both isolates were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequences of both organisms with the sequences of the type strains of most closely related Chryseobacterium species showed the highest sequence similarities of strains 100T and C26T to Chryseobacterium joostei (97.5%, 98.2%), Chryseobacterium viscerum (96.6%, 97.8%), Chryseobacterium gleum (97.1%, 97.7%), Chryseobacterium arthrosphaerae (97.3%, 97.7%), Chryseobacterium indologenes (97.2%, 97.7%), Chryseobacterium tructae (96.6%, 97.6%), Chryseobacterium jejuense (97.0%, 97.6%), Chryseobacterium oncorhynchi (96.3%, 97.5%), 16S rRNA gene sequence similarities to all other Chryseobacterium species were below 97.5%. The fatty acid profiles of both strains consisted of the major fatty acids C15:0 iso, C15:0 iso 2-OH/ C16:1 ω7c, C17:1 iso ω9c, and C17:0 iso 3-OH, but showed also slight differences (absence or presence of C16:0 3-OH, C15:1 isoF). DNA-DNA hybridizations between the two strains and to the type strains of C. joostei, C. indologenes, C. jejuense, C. tructae and C. viscerum resulted in similarity values clearly below 70%. These DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 100T and C26T represent novel species, for which the names Chryseobacterium gallinarum sp. nov. (type strain 100T = DSM 27622T = CCM 8493T), and Chyrseobacterium contaminans sp. nov. (type strain C26T = DSM 27621T = CCM 8492T) are proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
  • Julia Derichs, Peter Kämpfer, André Lipski
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    ABSTRACT: A Gram-negative, aerobic, rod-shaped, non-motile, non-spore forming bacterial strain, designated J22T, was isolated from a fresh pasta filled with porkmeat (tortellini) that was stored at 6°C. Based on the 16S rRNA gene sequence analysis strain J22T falls within the radiation of the Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest species are Pedobacter panaciterrae LMG 23400T (98.7%), Pedobacter africanus DSM 12126T (98.5%), and Pedobacter heparinus DSM 2366T (98.3%). The only isoprenoid quinone of strain J22T was menaquinone 7 (MK-7). The major fatty acids were iso-C15:0 (34.8%), C16:1 cis9 (24.9%), C16:0 (7.3%), C14:0 (4.8%) and iso-C17:0 3-OH (4.4%). These chemotaxonomic characteristics supported the assignment of the isolate to the genus Pedobacter. DNA-DNA hybridization experiments showed that the DNA-DNA similarity between strain J22T and its phylogenetic closest neighbor Pedobacter panaciterrae LMG 23400T was less than 36%. This indicated that the isolate was distinct from this species. Biochemical tests allowed phenotypic differentiation of strain J22T from the next related Pedobacter species. As a consequence, strain J22T should be classified as a representative of a novel species in the genus Pedobacter, for which the name Pedobacter nutrimenti sp. nov. is proposed. The type strain is J22T (= DSM 27372T = CCUG 64422T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
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    ABSTRACT: Four yellow pigmented strains (91A-561(T), 91A-576, 91A-593(T), and JM-1085(T)) isolated from plant materials, showed 97.2-98.7 % 16S rRNA gene sequence similarities among each other and were studied in a polyphasic approach for their taxonomic allocation. Cells of all four isolates were rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the four bacteria had highest sequence similarities to Chryseobacterium formosense (97.2-98.7 %), Chryseobacterium gwangjuense (97.1-97.8 %), and Chryseobacterium defluvii (94.6-98.0 %). Sequence similarities to all other Chryseobacterium species were below 97.5 %. Fatty acid analysis of the four strains showed Chryseobacterium typical profiles consisting of major fatty acids C15:0 iso, C15:0 iso 2-OH/C16:1 ω7c, C17:1 iso ω9c, and C17:0 iso 3-OH, but showed also slight differences. DNA-DNA hybridizations with type strains of C. gwangjuense, C. formosense, and C. defluvii resulted in values below 70 %. Isolates 91A-561(T) and 91A-576 showed DNA-DNA hybridization values >80 % indicating that they belonged to the same species; but nucleic acid fingerprinting showed that the two isolates represent two different strains. DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 91A-561(T) and 91A-576 represent a novel species, for which the name Chryseobacterium geocarposphaerae sp. nov. (type strain 91A-561(T)=LMG 27811(T)=CCM 8488(T)) is proposed. Strains 91A-593(T) and JM-1085(T) represent two additional new species for which we propose the names Chyrseobacterium zeae sp. nov. (type strain JM-1085(T)=LMG 27809(T), =CCM 8491(T)) and Chryseobacterium arachidis sp. nov. (type strain 91A-593(T)=LMG 27813(T), =CCM 8489(T)), respectively.
    Antonie van Leeuwenhoek 12/2013; · 2.07 Impact Factor

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Institutions

  • 1995–2014
    • Justus-Liebig-Universität Gießen
      • • Institute of Applied Microbiology
      • • Institut für Pharmakologie und Toxikologie
      Gieben, Hesse, Germany
  • 2013
    • Helmholtz Centre for Infection Research
      Brunswyck, Lower Saxony, Germany
  • 2005–2013
    • University of Bonn
      • • Institute of Nutrition and Food Science (IEL)
      • • Institute for Hygiene and Public Health
      Bonn, North Rhine-Westphalia, Germany
  • 2011
    • Universität Kassel
      • Institute of Biology
      Kassel, Hesse, Germany
  • 2010–2011
    • Bundesanstalt für Arbeitsschutz und Arbeitsmedizin
      Dortmund, North Rhine-Westphalia, Germany
    • University of Concepción
      Ciudad de Concepcion, Biobío, Chile
  • 2002–2011
    • University of Veterinary Medicine in Vienna
      • Institute of Bacteriology, Mycology and Hygiene
      Wien, Vienna, Austria
  • 2009
    • Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
      Jena, Thuringia, Germany
  • 2005–2009
    • National Chung Hsing University
      • Department of Soil and Environmental Sciences
      Taichung, Taiwan, Taiwan
  • 2008
    • Glasgow Caledonian University
      • Division of Biomedical Sciences
      Glasgow, SCT, United Kingdom
    • Eawag: Das Wasserforschungs-Institut des ETH-Bereichs
      Duebendorf, Zurich, Switzerland
    • Universität Osnabrück
      Osnabrück, Lower Saxony, Germany
  • 2007
    • Royal Perth Hospital
      Perth City, Western Australia, Australia
    • Johannes Gutenberg-Universität Mainz
      • Institute of Microbiology and Wine Research
      Mainz, Rhineland-Palatinate, Germany
  • 2003–2007
    • Ghent University
      • • Department of Clinical Biology, Microbiology and Immunology
      • • Laboratory of Microbiology
      Gent, VLG, Belgium
  • 2000–2007
    • Universität Stuttgart
      • Institute of Organic Chemistry
      Stuttgart, Baden-Württemberg, Germany
  • 2006
    • University of Vienna
      Wien, Vienna, Austria
  • 2004–2005
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2000–2003
    • University of Helsinki
      • Faculty of Veterinary Medicine
      Helsinki, Southern Finland Province, Finland
  • 1995–2002
    • Universität Ulm
      Ulm, Baden-Württemberg, Germany
    • RWTH Aachen University
      • Institut für Hygiene und Umweltmedizin
      Aachen, North Rhine-Westphalia, Germany
  • 1999
    • Technische Universität München
      • Chair of Technical Microbiology
      München, Bavaria, Germany
  • 1998
    • Centers for Disease Control and Prevention
      • National Center for Emerging and Zoonotic Infectious Diseases
      Druid Hills, GA, United States
  • 1988–1996
    • Technische Universität Berlin
      Berlín, Berlin, Germany
  • 1994
    • Bundesanstalt für Geowissenschaften und Rohstoffe
      Hanover, Lower Saxony, Germany
  • 1991
    • Hochschuelerschaft an der Technischen Universitaet Graz
      Berlin, New Hampshire, United States