Peter Kämpfer

Helmholtz Centre for Infection Research, Brunswyck, Lower Saxony, Germany

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Publications (358)763.34 Total impact

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    ABSTRACT: A Gram-positive staining, aerobic endospore forming bacterium (P-207T) was isolated from a rhizosphere soil sample in Auburn, Alabama, U.S.A.. On the basis of 16S rRNA gene sequence similarity comparisons, strain P-207T was grouped in the vicinity of representatives of the genera Virgibacillus, Ornithinibacillus, Cerasibacillus, Lentibacillus, and Oceanobacillus, but could not clearly be assigned to one of these genera. The highest similarity was found to the sequence of Virgibacillus carmonensis AJ316302 (94.4%), however, the 16S rRNA gene sequence similarity to the type strain of the type species of Virgibacillus, Virgibacillus pantothenticus was only 92.9%. The quinone system of strain P-207T consists of menaquinone MK-7, predominantly. The polar lipid profile exhibited the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and moderate to minor amounts of several unidentified phospholipids, glycolipids, phosphoglycolipids, an aminophospholipid and an aminolipid. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid and the polyamine pattern contained predominantly spermidine and spermine. The major fatty acids, which were C15:0 anteiso, C17:0 anteiso, C16:0 iso, and C15:0 iso. The G+C content of the genomic DNA was 34 mol%. Because of the low sequence similarity of strain P-207T to all representatives of Virgibacillus, Ornithinibacillus, Cerasibacillus, Lentibacillus, and Oceanobacillus, which was always < 95% and their unique lipid patterns, we propose that strain P-207T represents a novel species in a novel genus for which the name Pseudogracilibacillus auburnensis gen. nov. sp. nov. is proposed. The type strain is P-207T (= CCM 8509T = LMG 28212T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-positive, aerobic organisms, isolated from the healthy stem of a Zea mays plant was studied for its taxonomic position. Based on 16S rRNA gene sequence analysis strain JM-1068T was grouped into the genus Nocardioides, most closely related to Nocardioides alkalitolerans (97.1 %). The 16S rRNA gene sequence similarity to all other Nocardioides species was ≤ 96.1 %. The quinone system of strain JM-1068T contains the major menaquinone MK-8(H4). The diagnostic diaminoacid of the peptidoglycan is LL-diaminopimelic acid. In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids are predominant. The polyamine pattern contains predominantly spermidine and spermine. Fatty acid profile composed mainly of iso-branched and unsaturated fatty acids. This supported the allocation of the strain to the genus Nocardioides. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain JM-1068T from N. alkalitolerans and the most closely related species. JM-1068T represents a new species of the genus Nocardioides, for which we propose the name Nocardioides zeae sp. nov., with JM-1068T (= CIP 110696T = LMG 28079T) as the type strain.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2014; · 2.11 Impact Factor
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    ABSTRACT: ABSTRACT A novel myxobacterium MCy1366T (Ar1733) was isolated in 1981 from a soil sample collected from a region near Tokyo, Japan. It displayed general myxobacterial features like Gram negative staining, rod shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting body-like aggregates and myxospore-like structures. The strain was mesophilic, aerobic and showed chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like cephalosporin C, kanamycin, gentamycin, hygromycin B, polymyxin and bacitracin and the key fatty acids of whole cell hydrolysates were iso-C15:0, iso-C17:0, and iso-C17:0 2-OH. The genomic G+C content of the novel strain is 65.6 mol%. The 16S rRNA gene sequence showed highest similarity (97.60%) to "Stigmatella koreensis" strain KYC-1019 (not validly described taxon, GenBank accession no. EF112185). The phylogenetic analysis based on 16S rRNA gene sequences and MALDI-TOF data revealed a novel branch in the family Myxococcaceae. DNA-DNA hybridization showed only 28% similarity between the novel strain and the closest species, Corallococcus exiguus DSM 14696T (97% 16S rRNA gene sequence similarity). A recent isolate from a Switzerland soil sample and designated reference strain, MCy10622 displayed 99.9% 16S rRNA gene similarity and showed almost the same characteristics with MCy1366T. Since some morphological features like fruiting body like aggregates were barely reproducible in the type strain, the newly isolated strain MCy10622 was also intensively studied. On the basis of a comprehensive taxonomic study, we propose a novel genus and species, Aggregicoccus edonensis gen. nov., sp. nov., for strain MCy1366T and MCy10622. The type strain for the genus Aggregicoccus is MCy1366T (=DSM 27872T =NCCB 100468T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2014; · 2.11 Impact Factor
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    ABSTRACT: A Gram-staining-positive, aerobic, non-endospore forming organism, isolated as a seed endophyte (colonizing the internal healthy tissue of plant seed) of sweet corn (Zea mays) was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence similarity comparisons, strain CSE-5610T was grouped into the genus Cohnella, most closely related to Cohnella ginsengisoli GR21-5T (98.1 %) and Cohnella plantaginis YN-83T (97.5%). The 16S rRNA gene sequence similarity to other Cohnella species was < 96.6 %. DNA-DNA hybridization of strain CSE-5610T with Cohnella ginsengisoli DSM 18997T and Cohnella plantaginis DSM 25424T were 58% (reciprocal: 24%) and 30 (reciprocal: 27%), respectively. The fatty acid profile from whole cell hydrolysates supported the allocation of the strain to the genus Cohnella. Iso- and anteiso-branched fatty acids were found as major compounds. meso-Diaminopimelic acid was identified as cell wall diaminoacid. The quinone system consisted predominantly of menaquinone MK-7. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, a phospholipid and minor amounts of two polar lipids. In the polyamine pattern spermidine was the major polyamine. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain CSE-5610T from the two closely related species. Hence, CSE-5610T represents a new species of the genus Cohnella, for which we propose the name Cohnella rhizosphaerae sp. nov., with CSE-5610T (= LMG 28080T = CIP 110695T) as the type strain.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A yellow pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070T), was isolated as a presumptive endophyte from internal stem tissue of healthy corn stem. Phylogenetic analyses based on the 16S rRNA Gene sequence placed strain JM-1070T in the monophyletic cluster of the genus Mucilaginibacter with closest affiliation to the type strains of Mucilaginibacter composti (98%) and Mucilaginibacter calamicampi (97.2%). The 16S rRNA gene sequence similarity to all other Mucilaginibacter type strains was between 93.4 to 96.0%. DNA-DNA hybridizations of strain JM-1070T and the type strains of M. composti LMG 23497T and M. calamicampi CCUG 63418T showed low similarity values of 13% (reciprocal: 18%), and 52% (reciprocal 54.4%). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids were C15:0 iso (32.2%), C15:0 iso 2-OH/ C16:1 ω7c (measured as summed feature 3) (31.8%), and C17:0 iso 3-OH (15.1%) Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly sym-homospermidine. Characterization by 16S rRNA gene sequence, physiological parameters, polyamine, ubiquinone, polar lipid, and fatty acid composition revealed that strain JM-1070T represents a new species of the genus Mucilaginibacter. For this reason we propose the name Mucilaginibacter auburnensis sp. nov. with the type strain JM-1070T (= CIP 110694T = LMG 28078T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: A gram-negative, rod-shaped, oxidase positive, non-spore-forming, non-motile bacterium (strain 280T), isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus Paenochrobactrum group with a 16S rRNA gene sequence similarity of 98.8% to the both species, Paenochrobactrum gallinarii and Paenochrobactrum glaciei, respectively. This allocation was confirmed by the fatty acid data (major fatty acids: C18:1ω7c and C19:0 cyclo ω8c), polyamine pattern with the major compound putrescine and relatively high amounts of spermidine. Also the polar lipid profile with phosphatidylethanolamine, phosphatiylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholin and the genus-specific 'stretched aminolipid'; was well in line with the description of the genus Paenochrobactrum. The quinone system consists predominantly of ubiquinone Q-10 with traces of Q9 and Q11. DNA-DNA hybridization of strain 280T with Paenochrobactrum gallinarii Sa25T and Paenochrobactrum glaciei KMM 3858T showed similarity values of 38.8% (reciprocal 20.2%) and 30.2% (reciprocal 29.8%), respectively. These results in combination with differentiating physiological and biochemical data clearly showed that strain 280T merits species status. We propose the name Paenochrobactrum pullorum sp. nov. for this strain with the type strain 280T (= LMG 28095T = CIP 110700T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
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    ABSTRACT: Two yellow pigmented bacterial strains (100T and C26T), showing 98.4% 16S rRNA gene sequence similarity to each other and isolated from chicken in Germany and as a contaminant from an agar plate of a rhizosphere sample were studied in a polyphasic taxonomic approach. Cells of both isolates were rod shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequences of both organisms with the sequences of the type strains of most closely related Chryseobacterium species showed the highest sequence similarities of strains 100T and C26T to Chryseobacterium joostei (97.5%, 98.2%), Chryseobacterium viscerum (96.6%, 97.8%), Chryseobacterium gleum (97.1%, 97.7%), Chryseobacterium arthrosphaerae (97.3%, 97.7%), Chryseobacterium indologenes (97.2%, 97.7%), Chryseobacterium tructae (96.6%, 97.6%), Chryseobacterium jejuense (97.0%, 97.6%), Chryseobacterium oncorhynchi (96.3%, 97.5%), 16S rRNA gene sequence similarities to all other Chryseobacterium species were below 97.5%. The fatty acid profiles of both strains consisted of the major fatty acids C15:0 iso, C15:0 iso 2-OH/ C16:1 ω7c, C17:1 iso ω9c, and C17:0 iso 3-OH, but showed also slight differences (absence or presence of C16:0 3-OH, C15:1 isoF). DNA-DNA hybridizations between the two strains and to the type strains of C. joostei, C. indologenes, C. jejuense, C. tructae and C. viscerum resulted in similarity values clearly below 70%. These DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 100T and C26T represent novel species, for which the names Chryseobacterium gallinarum sp. nov. (type strain 100T = DSM 27622T = CCM 8493T), and Chyrseobacterium contaminans sp. nov. (type strain C26T = DSM 27621T = CCM 8492T) are proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
  • Julia Derichs, Peter Kämpfer, André Lipski
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    ABSTRACT: A Gram-negative, aerobic, rod-shaped, non-motile, non-spore forming bacterial strain, designated J22T, was isolated from a fresh pasta filled with porkmeat (tortellini) that was stored at 6°C. Based on the 16S rRNA gene sequence analysis strain J22T falls within the radiation of the Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest species are Pedobacter panaciterrae LMG 23400T (98.7%), Pedobacter africanus DSM 12126T (98.5%), and Pedobacter heparinus DSM 2366T (98.3%). The only isoprenoid quinone of strain J22T was menaquinone 7 (MK-7). The major fatty acids were iso-C15:0 (34.8%), C16:1 cis9 (24.9%), C16:0 (7.3%), C14:0 (4.8%) and iso-C17:0 3-OH (4.4%). These chemotaxonomic characteristics supported the assignment of the isolate to the genus Pedobacter. DNA-DNA hybridization experiments showed that the DNA-DNA similarity between strain J22T and its phylogenetic closest neighbor Pedobacter panaciterrae LMG 23400T was less than 36%. This indicated that the isolate was distinct from this species. Biochemical tests allowed phenotypic differentiation of strain J22T from the next related Pedobacter species. As a consequence, strain J22T should be classified as a representative of a novel species in the genus Pedobacter, for which the name Pedobacter nutrimenti sp. nov. is proposed. The type strain is J22T (= DSM 27372T = CCUG 64422T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
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    ABSTRACT: Four yellow pigmented strains (91A-561(T), 91A-576, 91A-593(T), and JM-1085(T)) isolated from plant materials, showed 97.2-98.7 % 16S rRNA gene sequence similarities among each other and were studied in a polyphasic approach for their taxonomic allocation. Cells of all four isolates were rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the four bacteria had highest sequence similarities to Chryseobacterium formosense (97.2-98.7 %), Chryseobacterium gwangjuense (97.1-97.8 %), and Chryseobacterium defluvii (94.6-98.0 %). Sequence similarities to all other Chryseobacterium species were below 97.5 %. Fatty acid analysis of the four strains showed Chryseobacterium typical profiles consisting of major fatty acids C15:0 iso, C15:0 iso 2-OH/C16:1 ω7c, C17:1 iso ω9c, and C17:0 iso 3-OH, but showed also slight differences. DNA-DNA hybridizations with type strains of C. gwangjuense, C. formosense, and C. defluvii resulted in values below 70 %. Isolates 91A-561(T) and 91A-576 showed DNA-DNA hybridization values >80 % indicating that they belonged to the same species; but nucleic acid fingerprinting showed that the two isolates represent two different strains. DNA-DNA hybridization results and the differentiating biochemical and chemotaxonomic properties showed, that both strains 91A-561(T) and 91A-576 represent a novel species, for which the name Chryseobacterium geocarposphaerae sp. nov. (type strain 91A-561(T)=LMG 27811(T)=CCM 8488(T)) is proposed. Strains 91A-593(T) and JM-1085(T) represent two additional new species for which we propose the names Chyrseobacterium zeae sp. nov. (type strain JM-1085(T)=LMG 27809(T), =CCM 8491(T)) and Chryseobacterium arachidis sp. nov. (type strain 91A-593(T)=LMG 27813(T), =CCM 8489(T)), respectively.
    Antonie van Leeuwenhoek 12/2013; · 2.07 Impact Factor
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    ABSTRACT: A Gram-positive non endospore-forming rod, Ca11T was isolated from a bioreactor with extensive phosphorus removal and was studied in detail for its taxonomic allocation. 16S rRNA gene sequence analysis revealed closest sequence similarity of the strain to type strains of 'Corynebacterium' hoagii and Rhodococcus equi (98.9%), Rhodococcus koreensis and Rhodococcus wratislaviensis (both 98.4%), Rhodococcus opacus and Rhodococcus canchipurensi (both 98.0%) followed by Rhodococcus kunmingensis and Rhodococcus imtechensi (97.7%). Phylogenetic trees showed a distinct clustering of strain Ca11T with the type strains of C. hoagii, R. equi, and R. kunmingensis separated from all other Rhodococcus species. The quinone systems of strain Ca11T was composed of dihydrogenated menaquinones with 8 (major amount) as well as 7 and 6 isoprenoid units [MK-8(H2), MK-7(H2), MK-6(H2)]. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamin, phosphatidylinositol, phosphatidylinositol mannoside, one unknown phospholipid and a glycolipid. The fatty acid profile was similar to that reported for R. equi and contained major amounts of C16:0, C18:1 ω9c and 10-methyl C18:0, supporting the grouping of the strain to the genus Rhodococcus. Physiological, biochemical characterisation and DNA-DNA hybridizations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of the isolate. On the basis of these results, a novel species is proposed for strain Ca11T (= DSM 45893T = LMG 27563T), with the name Rhodococcus defluvii sp. nov. In addition, a polyphasic taxonomic analysis of 'Corynebacterium' hoagii DSM 20925T and Rhodococcus equi DSM 20669T indicated that the two strains belong to the same species for which the name Rhodococcus hoagii has priority according to the rules of the bacteriological code.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2013; · 2.11 Impact Factor
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2013; 63(Pt 11):4370. · 2.11 Impact Factor
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    ABSTRACT: Four isolates of Gram-negative facultatively anaerobic bacteria, three of them producing NDM-1 carbapenemase, were isolated from hospitalized patients and outpatients attending two military hospitals in Rawalpindi, Pakistan, and studied for their taxonomic position. Initially the strains were phenotypically identified as Citrobacter species. Comparative analysis of 16S rRNA gene sequences then showed that the four strains shared >97%, but in no case >98.3%, 16S rRNA gene sequence similarities to members of the genera Citrobacter, Kluyvera, Pantoea, Enterobacter and Raoultella, but always formed a separate cluster in respective phylogenetic trees. Based on multilocus sequence analysis (MLSA) including partial recN, rpoA, thdF and rpoB gene sequence and respective amino acid sequence analysis it turned out that the strains also here always formed separate clusters. Based on further comparative analyses including DNA–DNA hybridizations, genomic fingerprint analysis using rep- and RAPD-PCRs and physiological tests, it is proposed to classify these four strains into the novel genus Pseudocitrobacter gen. nov. with a new species Pseudocitrobacter faecalis sp. nov. with strain 25 CITT (= CCM 8479T = LMG 27751T) and Pseudocitrobacter anthropi sp. nov. with strain C138T (= CCM 8478T = LMG 27750T), as the type strains, respectively.
    Systematic and Applied Microbiology 10/2013; · 3.29 Impact Factor
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    ABSTRACT: Four isolates of Gram-negative facultatively anaerobic bacteria, three of them producing NDM-1 carbapenemase, were isolated from hospitalized patients and outpatients attending two military hospitals in Rawalpindi, Pakistan, and studied for their taxonomic position. Initially the strains were phenotypically identified as Citrobacter species. Comparative analysis of 16S rRNA gene sequences then showed that the four strains shared >97%, but in no case >98.3%, 16S rRNA gene sequence similarities to members of the genera Citrobacter, Kluyvera, Pantoea, Enterobacter and Raoultella, but always formed a separate cluster in respective phylogenetic trees. Based on multilocus sequence analysis (MLSA) including partial recN, rpoA, thdF and rpoB gene sequence and respective amino acid sequence analysis it turned out that the strains also here always formed separate clusters. Based on further comparative analyses including DNA-DNA hybridizations, genomic fingerprint analysis using rep- and RAPD-PCRs and physiological tests, it is proposed to classify these four strains into the novel genus Pseudocitrobacter gen. nov. with a new species Pseudocitrobacter faecalis sp. nov. with strain 25 CIT(T) (=CCM 8479(T)=LMG 27751(T)) and Pseudocitrobacter anthropi sp. nov. with strain C138(T) (=CCM 8478(T)=LMG 27750(T)), as the type strains, respectively.
    Systematic and Applied Microbiology 10/2013; · 3.29 Impact Factor
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    ABSTRACT: A Gram-positive, non-spore-forming bacterium (RV113T) was isolated from the marine sponge Aplysina aerophoba. The 16S rRNA gene sequence analysis and similarity studies showed that strain RV113T belongs to the genus Rubrobacter, and is most closely related to Rubrobacter bracariensis VF70612 S1T (96.9%) and more distantly related (< 93%) to all other Rubrobacter species. The peptidoglycan diaminoacid was lysine. Strain RV113T exhibited a quinone system with the predominant compound menaquinone MK-8. The polar lipid profile of RV113T consisted of the major compounds phosphatidylglycerol and two unidentified phosphoglycolipids. The major fatty acid was anteiso C17:0ω9. These chemotaxonomic traits are in excellent agreement with those of other Rubrobacter species. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain RV113T from all other Rubrobacter species. RV113T merits species status, and we propose the name Rubrobacter aplysinae sp. nov. with the type strain RV113T (= DSM 27440T = CECT 8425T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2013; · 2.11 Impact Factor
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    ABSTRACT: In 2000 an Actinomycetes strain was isolated from a soil sample, which showed a strong antibacterial activity in its culture extracts. The antibiotic activity corresponds to a lipopeptide complex which was named Friulimycin basing on the fact that the producing microorganism was isolated from a soil sample from the region Friaul in Italy. Taxonomic investigations showed that the producer strain belongs to the genus Actinoplanes, for which the name Actinoplanes friuliensis was proposed. During further taxonomic studies another antibiotic producing Actinoplanes isolate (FH 2241T) was characterized, for which the name Actinoplanes nipponensis was proposed in a patent. This organism was shown to be related to Actinoplanes friuliensis. Actinoplanes nipponensis was never described in detail and the name was never validly published and here we present a complete description of Actinoplanes nipponensis sp. nov. and an emended description Actinoplanes friuliensis with the type strains (HAG 010964T = ATCC 31145T = DSM 43867T) and (FH 2241T = DSM 45797T = CCUG 63250T), respectively.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2013; · 2.11 Impact Factor
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    ABSTRACT: Two black-pigmented anaerobic bacterial, designated LMM 40T and LMM 41, were isolated from the bovine postpartum endometrium of two Holstein cows. The 16S rRNA gene sequences of the two strains were identical and showed highest similarity to the 16S rRNA gene sequence of Porphyromonas crevioricanis (90.2%) but only 85.1% 16S rRNA gene sequence similarity to the type species of the genus Porphyromonas, Porphyromonas asaccharolytica. The major fatty acid profiles of the two strains were similar to those of Porphyromonas species, containing iso-C15:0 as the major component and moderate amounts of anteiso-C15:0, iso-C13:0, C15:0 and C16:0. Hydroxylated fatty acids, such as iso-C14:0 3-OH, iso-C16:0 3-OH and iso-C17:0 3-OH, were also detected. The quinone profiles were dominated by the menaquinones MK-8 and MK-9 while spermidine was the major polyamine. The polar lipid profiles contained major amounts of phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminophospholipid and two unidentified lipids and minor amounts of phosphatidylglycerol, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified glycolipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The genomic DNA G+C content of LMM 40T and LMM 41 is 40.7mol% and 41.3mol%, respectively. Based on a polyphasic approach, including phylogenetic analysis, physiological and biochemical tests as well as metabolic fingerprinting, it is proposed that the two strains are members of a novel genus, for which the name Falsiporphyromonas endometrii gen. nov., sp. nov. is proposed. Type strain is LMM 40T (= DSM 27210T = CCUG 64267T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2013; · 2.11 Impact Factor
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    ABSTRACT: Two strains, 8-4-E12T and 8-4-E13T were isolated from a bio-waste composting reactor. Based on the 16S rRNA gene sequences, both strains belong to the genus Bacillus. Strain 8-4-E12T was most closely related to the type strains of B. shackletonii, B. acidicola, B. sporothermodurans and B. oleronius (96.4, 96.3, 96.0 and 95.6 % 16S rRNA gene similarity, respectively), whereas strain 8-4-E13T was most closely related to B. humi (96.5 % sequence similarity). Strains 8-4-E12T and 8-4-E13T shared a 16S rRNA gene sequence similarity of 94 %. The fatty acid profile of strain 8-4-E12T was dominated by saturated iso- and anteiso-branched fatty acids (iso-C15:0, anteiso-C15:0, anteiso-C17:0, iso-C16:0), and also contained considerable amounts of C16:0. The fatty acid profile of strain 8-4-E13T showed a predominance of iso-C15:0 (65 %), with lower amounts of other saturated branched-chain fatty acids along with an unsaturated alcohol. Both strains contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids. Additionally, strain 8-4-E12T contained an unknown lipid and strain 8-4-E13T two unknown (amino-) phospholipids. The diagnostic diamino acid found in the cell wall peptidoglycan of 8-4-E12T and 8-4-E13T was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The results of physiological and biochemical tests also allowed a phenotypic differentiation of both strains from each other and from related Bacillus species. On the basis of their phylogenetic, phenotypic and chemotaxonomic properties, the strains 8-4-E12T and 8-4-E13T represent novel species of the genus Bacillus, for which the names Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov. are proposed. The type strains of the two novel species are the strains 8-4-E12T (= DSM 23947T = LMG 27601) and 8-4-E13T (= DSM 23948T = LMG 27602), respectively.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2013; · 2.11 Impact Factor
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    ABSTRACT: Two groups of Gram-positive, aerobic bacterial strains were isolated from cow raw milk, from a milking machine and from bulk tank milk. Based on their 16S rRNA gene sequences these isolates formed two distinct groups within the genus Corynebacterium. The sequence similarities of the isolates to the type strains of the genus Corynebacterium were below 98.4%. The presence of menaquinones MK-8(H2) and MK-9(H2), the predominant fatty acid 18:1 cis9, a polar lipid pattern with several phospholipids but without aminolipids was in accord with the characteristics of this genus. The results of DNA-DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of the strains from all known Corynebacterium species. Therefore, the isolates were assigned to two new species of this genus for which the names Corynebacterium frankenforstense sp. nov (type strain ST18 T = DSM 45800T = CCUG 63371T), and Corynebacterium lactis sp. nov. (type strain RW2-5 T = DSM 45799T = CCUG 63372T) are proposed, respectively.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2013; · 2.11 Impact Factor
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    ABSTRACT: Strain BCT-7112(T) was isolated in 1966 in Japan from a survey designed to obtain naturally occurring microorganisms as pure cultures in the laboratory for use as probiotics in animal nutrition. This strain, which was primarily identified as Bacillus cereus var toyoi, has been in use for more than 30 years as the active ingredient of the preparation TOYOCERIN(®), an additive for use in animal nutrition (e.g. swine, poultry, cattle, rabbits and aquaculture). Despite the fact that the strain was initially classified as B. cereus, it showed significant genomic differences from the type strains of the B. cereus group that were large enough (ANI values below 92%) to allow it to be considered as a different species within the group. The polyphasic taxonomic study presented here provides sufficient discriminative parameters to classify BCT-7112(T) as a new species for which the name Bacillus toyonensis sp. nov. is proposed, with BCT-7112(T) (=CECT 876(T); =NCIMB 14858(T)) being designated as the type strain. In addition, a pairwise comparison between the available genomes of the whole B. cereus group by means of average nucleotide identity (ANI) calculations indicated that besides the eight classified species (including B. toyonensis), additional genomospecies could be detected, and most of them also had ANI values below 94%. ANI values were on the borderline of a species definition only in the cases of representatives of B. cereus versus B. thuringiensis, and B. mycoides and B. weihenstephanensis.
    Systematic and Applied Microbiology 06/2013; · 3.29 Impact Factor
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    ABSTRACT: A gram-negative, rod-shaped, oxidase positive, non-spore-forming, non-motile bacterium (B1315T) was isolated from the placenta of a sheep with abortion. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the Brucella-Ochrobactrum-Paenochrobactrum-Pseudochrobactrum group with 94.5-94.8%, 94.3-96.1%, 95.0-95.1%, and 95.9-96.1% sequence similarities to type strains of the four genera, respectively. Phylogenetic trees indicate a close relationship to the type strains of Ochrobactrum gallinifaecis and Ochrobactrum oryzae (95.9. and 96.1% sequence similarity, respectively). Chemotaxonomic data confirmed the allocation of strain B1315T to the family Brucellaceae (quinone system: ubiquinone Q-10 and major fatty acids: C18:1ω7c and C19:0 cyclo ω;8c). The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and the unidentified but unique phospholipid PL7. The polyamine pattern of B1315T contained the major polyamines spermidine and putrescine. On the basis of the 16S rRNA gene and recA sequence phylogeny and chemotaxonomic data isolate B1315T is clearly different from the genera Brucella, Ochrobactrum, Paenochrobactrum and Pseudochrobactrum. On the basis of these data we propose the novel genus Falsochrobactrum gen. nov. with the type species Falsochrobactrum ovis sp. nov. with the type strain B1315T (= CCM 8460T = LMG 27356T) The taxonomic allocation of O. gallinifaecis, which grouped inconsistently together with B1315T on the basis of 16S rRNA gene sequence data, but shows the chemotaxonomic features of Ochrobactrum, remains to be clarified.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor

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6k Citations
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Institutions

  • 2013
    • Helmholtz Centre for Infection Research
      Brunswyck, Lower Saxony, Germany
  • 2011–2013
    • University of Bonn
      • Institute of Nutrition and Food Science (IEL)
      Bonn, North Rhine-Westphalia, Germany
    • Universität Kassel
      • Institute of Biology
      Kassel, Hesse, Germany
  • 1995–2013
    • Justus-Liebig-Universität Gießen
      • • Institut für Angewandte Mikrobiologie
      • • Institut für Pharmakologie und Toxikologie
      Gießen, Hesse, Germany
  • 2010–2011
    • Bundesanstalt für Arbeitsschutz und Arbeitsmedizin
      Dortmund, North Rhine-Westphalia, Germany
    • University of Concepción
      Ciudad de Concepcion, Biobío, Chile
  • 2002–2011
    • University of Veterinary Medicine in Vienna
      • Institute of Bacteriology, Mycology and Hygiene
      Wien, Vienna, Austria
  • 2009
    • Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
      Jena, Thuringia, Germany
  • 2005–2009
    • National Chung Hsing University
      • Department of Soil and Environmental Sciences
      Taichung, Taiwan, Taiwan
  • 2008
    • Glasgow Caledonian University
      • Division of Biomedical Sciences
      Glasgow, SCT, United Kingdom
    • Eawag: Das Wasserforschungs-Institut des ETH-Bereichs
      Duebendorf, Zurich, Switzerland
    • Universität Osnabrück
      Osnabrück, Lower Saxony, Germany
  • 2007
    • Royal Perth Hospital
      Perth City, Western Australia, Australia
    • Johannes Gutenberg-Universität Mainz
      • Institute of Microbiology and Wine Research
      Mainz, Rhineland-Palatinate, Germany
  • 2003–2007
    • Ghent University
      • • Department of Clinical Biology, Microbiology and Immunology
      • • Laboratory of Microbiology
      Gent, VLG, Belgium
  • 2000–2007
    • Universität Stuttgart
      • Institute of Organic Chemistry
      Stuttgart, Baden-Württemberg, Germany
  • 2006
    • University of Vienna
      Wien, Vienna, Austria
  • 2004–2005
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2000–2003
    • University of Helsinki
      • Faculty of Veterinary Medicine
      Helsinki, Southern Finland Province, Finland
  • 1995–2002
    • Universität Ulm
      Ulm, Baden-Württemberg, Germany
    • RWTH Aachen University
      • Institut für Hygiene und Umweltmedizin
      Aachen, North Rhine-Westphalia, Germany
  • 1999
    • Technische Universität München
      • Chair of Technical Microbiology
      München, Bavaria, Germany
  • 1998
    • Centers for Disease Control and Prevention
      • National Center for Emerging and Zoonotic Infectious Diseases
      Druid Hills, GA, United States
  • 1988–1996
    • Technische Universität Berlin
      Berlín, Berlin, Germany
  • 1994
    • Bundesanstalt für Geowissenschaften und Rohstoffe
      Hanover, Lower Saxony, Germany
  • 1991
    • Hochschuelerschaft an der Technischen Universitaet Graz
      Berlin, New Hampshire, United States