T D Vlasov

Publications (30)1 Total impact

• Article: DISTRIBUTION OF NEUROGLOBIN IN RAT FOREBRAIN STRUCTURES AFTER TRANSITORY ISCHAEMIA

  I.P. Grigorev, E.G. Gilerovich, O.V. Kirik, T.D. Vlasov, D.E. Korzhevskii
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  ABSTRACT: The distribution of the heme-containing protein, neuroglobin, in the rat forebrain was examined using immunohistochemistry in normal rats and in rats 48 h after transitory ischaemia induced by endovascular occlusion of the middle cerebral artery for 30 min. In rats with transitory ischaemia, an uneven distribution of neuroglobin was observed in the forebrain: maximal neuroglobin immunoreactivity was found in the cortex, caudate nucleus, choroid plexus, and ependymal lining, whereas the least neuroglobin immunoreactivity occurred in neural pathways. Unilateral brain ischaemia results in a profound decrease in neuroglobin immunoreactivity in the lesioned hemisphere. No increased expression of neuroglobin was detected in the penumbra or at points located distal from the lesioned site. Key words: brain, neuroglobin, immunohistochemistry, ischaemia.
  Journal of Asymmetry. 04/2013; 7(1):15-21.
• Article: [Structural organization of striatal microgliocytes following focal ischemia].

  D E Korzhevskiĭ, O V Kirik, E G Sukhorukova, T D Vlasov
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  ABSTRACT: Microgliocytes are currently known as structurally labile cells, that, by changing the length of their processes, control the synaptic organization of the brain. The aim of this study was to investigate structural organization of microglia of the striatum and of the forebrain periventricular area in rat following transitory focal ischemia. In the absence of brain infarction, ischemic lesion was found to activate microglia, which contributed to rearrangement of striatal neuropil. As microgliocytes became activated, they changed their shape from characteristic dendritic one to oval and rounded, which are typical to amoeboid microglia. Microgliocytes were shown to be capable of proliferation.
  Morfologiia (Saint Petersburg, Russia) 01/2012; 141(2):28-32.
• Article: Simulation of unilateral ischemic injury to the striatal neurons inflicted by short-term occlusion of the middle cerebral artery.

  D E Korzhevskii, O V Kirik, A E Baisa, T D Vlasov
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  ABSTRACT: The reproducibility of brain injury was evaluated by simulating ischemia in rats by 30-min occlusion of the middle cerebral artery. The selected ischemia-reperfusion protocol was characterized by high reproduction of the striatal neuron injury, which fact suggests this model for studies of nerve tissue reactions to injury and for evaluation of the efficiency of neuroprotective drugs.
  Bulletin of Experimental Biology and Medicine 03/2009; 147(2):255-6. · 0.27 Impact Factor
• Article: Induction of nestin synthesis in rat brain cells by ischemic damage.

  D E Korzhevskii, M V Lentsman, A V Gilyarov, O V Kirik, T D Vlasov
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  ABSTRACT: Nestin, an intermediate filament protein, is known to be expressed in proliferating and provisional cells in the forming mammalian brain, disappearing on differentiation. The aim of the present work was to identify the morphological types and locations of cells regaining the ability to synthesize nestin after transient total brain ischemia in rats. Transient ischemia was found to be followed by the induction of nestin synthesis in astrocytes in the damaged area; these cells acquired structural features not characteristic of the adult brain, and these persisted in the long term. Nestin synthesis was also induced in proliferation-capable undifferentiated cells in the subventricular zone. The acquisition by astrocytes of some of the phenotypic features of immature glial cells, however, does not provide grounds for the notion that they were transformed into neural stem cells.
  Neuroscience and Behavioral Physiology 03/2008; 38(2):139-43.
• Article: [Ischemic damage-induced nestin synthesis in the rat brain cells].

  D E Korzhevskiĭ, M V Lentsman, A V Giliarov, O V Kirik, T D Vlasov
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  ABSTRACT: Nestin, an intermediate filament protein, is known to be expressed in the proliferating and provisional cells of the developing mammalian brain and is lost during differentiation. The aim of the present study was to determine the morphological type and localization areas of the rat brain cells exhibiting the ability to synthesize nestin after a short-term global ischemia of the brain. Induction of nestin synthesis after a short-term ischemia was found within the injured brain areas in astrocytes, which exhibited structural features atypical for these cells in mature brain and maintained them for a long time, and in the morphologically undifferentiated cells of subventricular zone, which were able to proliferate. However, acquisition by astrocytes of some phenotypic properties of immature glial cell does not by itself support the view that they may transform into neural stem cells.
  Morfologiia (Saint Petersburg, Russia) 02/2007; 131(1):23-6.
• Article: [The role of oxygen free radicals in the mechanisms of local and distant ischemic myocardial preconditioning].

  N N Petrishchev, E V Shliakhto, V A Tsyrlin, T D Vlasov, A V Syrenskiĭ, M M Galagudza
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  ABSTRACT: The phenomenon of ischemic preconditioning consists in increase in myocardial tolerance to ischemia after short periods of ischemia and reperfusion of the myocardium (local preconditioning) or a distant organ (distant preconditioning). This study is dedicated to the role of oxygen free radicals (OFR) in the mechanisms of preconditioning; pharmacological inhibition of OFR with N-2-mercaptopropionylglycin, a synthetic anti-oxidant, was used. Mouse experiments demonstrated a reduction in the infarction-limiting effect of local preconditioning as a result of OFR inhibiting. This confirms the hypothesis according to which OFR play an important role in the mechanisms of launching and mediating the protective effect of local preconditioning. On the contrary, application of the same dose of N2-mercaptopropionylglycin does not lead to a significant weakening of the infarction-limiting effect of distant preconditioning caused by 20 min of ischemia and 15 min of reperfusion of the small intestine. Thus, it is unlikely that OFR participate in the realization of the effect of distant preconditioning caused by small intestinal ischemia. The authors discuss hypothetic molecular mechanisms of distant myocardial preconditioning.
  Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 02/2006;
• Article: Effects of helium-neon laser irradiation and local anesthetics on potassium channels in pond snail neurons.

  Yu D Ignatov, A I Vislobokov, T D Vlasov, M E Kolpakova, K N Mel'nikov, I N Petrishchev
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  ABSTRACT: Intracellular dialysis and membrane voltage clamping were used to show that He-Ne laser irradiation of a pond snail neuron at a dose of 0.7 x 10(-4) J (power density 1.5 x 10(2) W/m2) increases the amplitude of the potential-dependent slow potassium current, while a dose of 0.7 x 10(-3) J decreases this current. Bupivacaine suppresses the potassium current. Combined application of laser irradiation at a dose of 0.7 x 10(-3) J increased the blocking effect of 10 microM bupivacaine on the slow potassium current, while an irradiation dose of 0.7 x 10(-4) J weakened the effect of bupivacaine.
  Neuroscience and Behavioral Physiology 11/2005; 35(8):871-5.
• Article: Effects of Helium-Neon Laser Irradiation and Local Anesthetics on Potassium Channels in Pond Snail Neurons

  Yu. D. Ignatov, A. I. Vislobokov, T. D. Vlasov, M. E. Kolpakova, K. N. Mel’nikov, I. N. Petrishchev
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  ABSTRACT: Intracellular dialysis and membrane voltage clamping were used to show that He-Ne laser irradiation of a pond snail neuron at a dose of 0.7⋅10−4 J (power density 1.5⋅102 W/m2) increases the amplitude of the potential-dependent slow potassium current, while a dose of 0.7⋅10−3 J decreases this current. Bupivacaine suppresses the potassium current. Combined application of laser irradiation at a dose of 0.7⋅10−3 J increased the blocking effect of 10 M bupivacaine on the slow potassium current, while an irradiation dose of 0.7⋅10−4 J weakened the effect of bupivacaine.
  Neuroscience and Behavioral Physiology 09/2005; 35(8):871-875.
• Article: Ischemic preconditioning of the rat brain as a method of endothelial protection from ischemic/repercussion injury.

  T D Vlasov, D E Korzhevskii, E A Polyakova
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  ABSTRACT: The studies reported here addressed the endothelium-protecting action of local and remote ischemic preconditioning of the brain in rats. Cerebral ischemia lasting 30 min was reproduced by thermocoagulation of the vertebral arteries with simultaneous clamping of the carotid arteries, the procedure being followed by reperfusion via the carotid arteries for 120 min (controls). The early and late phases of ischemic preconditioning and remote preconditioning were reproduced. Brain blood flow was recorded using high-frequency Doppler ultrasonography. The early and late phases of local ischemic preconditioning and the late phase of remote ischemic preconditioning were found to have endothelium-protecting actions apparent as improvements in the recovery of brain blood flow in the post-ischemic period in preconditioned rats, with lower levels of endothelial desquamation and cerebral edema. Blockade of nitric oxide synthesis eliminated the protective effects of both phases of preconditioning.
  Neuroscience and Behavioral Physiology 08/2005; 35(6):567-72.
• Article: [Influence of He-Ne laser irradiation and local anesthetics on potassium channels in the snail neurons].

  Iu D Ignatov, A I Vislobokov, T D Vlasov, M E Kolpakova, K N Mel'nikov, I N Petrishchev
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  ABSTRACT: Using clamp method it had been shown that He-Ne laser irradiation of the snail neurons increases the amplitude of voltage-gated slow potassium currents in dose of 0.7 x 10(-4) (fluence 1.5 x 10(2) Wt/m2) and decreases it in dose 0.7 x 10(-3). Bupivacaine and lidocaine suppressed potassium currents. Laser irradiation in dose 0.7 x 10(-3) enhanced the inhibitory effect of bupivacaine (10 mcM) and in dose 0.7 x 10(-4) it decreased the inhibitory effect of bupivacaine. The results of the study suggest mechanisms of the He-Ne laser irradiation effect in combination with pharmacological substances on ion channels of electrically excitable cells.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2004; 90(9):1113-20.
• Article: [Myocardial ischemic postconditioning: a brief ischemia causes conversion of resistent reperfusion-induced ventricular fibrillation into the normal rhythm].

  N N Petrishchev, T D Vlasov, M M Galagudza, D I Kurapeev, S M Minasian
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  ABSTRACT: Brief episodes of myocardial ischemia-reperfusion were shown to be protective against reperfusion injury when used during early reperfusion after a prolonged ischemic episode. This phenomenon has been termed myocardial ischemic postconditioning. In this study, an effect of ischemic postconditioning on persistent reperfusion-induced ventricular fibrillation was studied in the rat isolated heart. 2 minutes of global ischemia on the 15th minute of reperfusion after 30 minutes of regional ischemia effectively abolished the persistent ventricular fibrillation. In non-postconditioned hearts, the ventricular fibrillation continued to the end of reperfusion. The ischemic postconditioning seems to exert a strong antiarrhythmic effect protecting the heart against persistent reperfusion-induced ventricular tachyarrhythmias.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2004; 90(9):1138-44.
• Article: [Ischemic adaptation of the rat brain as a method for protection of endothelium from ischemic reperfusion injury].

  T D Vlasov, D E Korzhevskiĭ, E A Poliakova
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  ABSTRACT: This study represents results of investigation carried out to determine the endothelium-protective effect of early and late phases of brain ischemic preconditioning as well as local and remote adaptation. The experiments were performed on adult male rats. Prolonged 30-min four vessels brain ischemia followed by 120-min reperfusion on carotid arteries, was performed (control group). Early and late local ischemic preconditioning was due to both 5-min ischemia and 30-min and 48 h reperfusion respectively on carotid arteries. Remote ischemic preconditioning was caused by 30-min ischemia and also by 15-min and 48 h reperfusion, respectively (early and late phases of adaptation) on femoral artery before prolonged brain ischemia described above. To estimate the role of nitric oxide in ischemic adaptation, mechanisms involved both nonselective blocker of NO-synthesis (N omega-nitro-L-arginine) in the time of early adaptation phase and the relatively selective iNOS inhibitor S-methylisothiourea sulfate, given before sustained brain ischemia, on the late preconditioning. Registration of brain blood flow was made by ultrasonic high-frequency Doppler device. Degree of brain edema was studied and evaluation of desquamated endothelial cells in blood was carried out. Early and late phases of local ischemic preconditioning were found to improve the brain blood flow and level of circulatory endothelial cells as well as to reduce degree of edema. The endothelium-protective effect of remote ischemic preconditioning has been proved in this study only on the late phase. Nitric oxygen was found to be important endothelium-protective factor in ischemic preconditioning.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 02/2004; 90(1):40-8.
• Article: [Effect of the He-Ne laser irradiation on resistance of the isolated heart to the ischemic and reperfusion injury].

  M E Kolpakova, T D Vlasov, N N Petrishchev, A I Vislobokov
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  ABSTRACT: The aim of this work was to investigate the myocardial protection against ischemia/reperfusion using low level laser irradiation (LLLI). It has been shown that pulse pressure was higher in the period of post-ischemic reperfusion as compared with the control group. It provided a better restoration of myocardial contractility as well as increasing of coronary flow in the reperfusion period. The amount of ventricular rhythm disorder episodes decreased. These effects of laser application were registered in conditions of coronary flow reduction less than 50%. One of the suggested mechanisms of laser effect is an ATP-sensitive channel activation.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 01/2004; 89(12):1496-502.
• Article: Frequency-dependent effects of low-intensity ultrasound on activity of isolated heart.

  N N Petrishchev, T D Vlasov, M M Galagudza, Yu N Makov, C M Minasyan
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  ABSTRACT: The experiments with low-intensity ultrasound stimulation of isolated rat heart revealed frequency-dependent effects of ultrasound in a frequency range of 45-298 kHz on cardiac activity.
  Bulletin of Experimental Biology and Medicine 10/2003; 136(3):239-41. · 0.27 Impact Factor
• Article: Effect of low-frequency low-intensity ultrasound on contractile function of isolated heart.

  N N Petrishchev, T D Vlasov, M M Galagudza, Yu N Makov
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  ABSTRACT: Stimulation of the isolated heart with low-intensity low-frequency ultrasound produced a positive inotropic effect (at certain ultrasound intensity). This effect manifested in increased systolic and pulse intraventricular pressures and persisted for tens of minutes after the end of sonication.
  Bulletin of Experimental Biology and Medicine 05/2002; 133(4):327-9. · 0.27 Impact Factor
• Article: [Effect of angiotensin converting enzyme inhibitor spirapril on dimensions of experimental myocardial infarction, development of ischemic tachyarrhythmias, and ischemic adaptation of the heart].

  V A Tsyrlin, E V Shliakhto, E M Nifontov, T D Vlasov, A V Syrenskiĭ, D L Sonin, M M Galagudza
  Kardiologiia 02/2002; 42(1):91-5. · 0.20 Impact Factor
• Article: [Myocardial ischemic preconditioning: pathophysiological mechanisms and prospects of clinical application (a literature review)].

  N N Petrishchev, E V Shliakhto, T D Vlasov, M M Galagudza
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  ABSTRACT: Ischemic preconditioning (IPC, i.e. increase in the organ resistance to a prolonged ischemia which occurs after a brief ischemic challenge) seems to be one of the most powerful endogenous cardioprotective mechanisms known to date. Current data regarding molecular mechanisms of early (classic) IPC as well as the second window of protection are reviewed in the context of the concept of sequential three-staged development of protective effect. Based on original and published data, possible mechanisms of remote IPC are considered. The review comprises current ideas of existence of the IPC clinical correlates and its use in clinic.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 06/2001; 87(5):688-705.
• Article: [Endothelium disfunction in patients with myocardial infarction at the younger age].

  E V Shliakhto, E A Bazhenova, O A Berkovich, E V Volkova, T D Vlasov, I A Tolstova, M Z Alugashvili
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  ABSTRACT: The endothelium was studied in males who had sustained myocardial infarction (MI) at young age (under 45 years). The parameters of endothelium-dependent dilatation of the brachial artery were estimated during a reactive hyperemia test and the blood count of circulating (desquamated) endotheliocytes was measured. The values obtained in patients groups randomized by different parameters were compared with each other and with control values. The patients who had experienced in youth were found to have endothelial dysfunction, as appeared as both a larger number of circulating blood endotheliocytes and an altered vascular response during the reactive hyperemia test. It was found that endothelial function was not clearly related to the risk factors of atherosclerosis though the association was more marked when coronary heart disease is concurrent with arterial hypertension and hypercholesterolemia.
  Vestnik Rossiĭskoĭ akademii meditsinskikh nauk / Rossiĭskaia akademiia meditsinskikh nauk 02/2001;
• Article: [Adaptation of the rat small intestine to ischemia].

  T D Vlasov, D A Smirnov, G M Nutfullina
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  ABSTRACT: Pre-conditioned rats underwent one cycle of intestinal ischemia/reperfusion with subsequent assessment of the heart remote ischemic pre-conditioning (IPC). A local IPC exerted an obvious protective action whereas a remote IPC was ineffective. The local IPC depended partially on the nitric oxide (NO) synthesis. L-arginine administration reduced the intestinal injury. The findings suggest that the ischemic pre-conditioning of intestine depends partially on the NO synthesis and that the remote IPC in intestine does not exist.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 02/2001; 87(1):118-24.
• Article: [Diagnostic value of evaluation of desquamated endothelial cells in blood].

  N N Petrishchev, O A Berkovich, T D Vlasov, E V Volkova, E E Zueva, E V Mozgovaia
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  ABSTRACT: Circulating endotheliocytes as indicator of endothelial dysfunction were evaluated in the blood by Hladovec's method (1978) in patients with coronary disease, coronary stroke, insulin-dependent diabetes mellitus, and gestosis. The content of circulating (desquamated) endotheliocytes was increased in all patients, which reflected endothelial damage. The most pronounced changes were detected in patients with gestosis. Endotheliocyte content correlated with the concentration of Willebrandt's factor in the blood, which recommends using endotheliocyte count as a marker of endothelial damage.
  Klinicheskaia laboratornaia diagnostika 02/2001;