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ABSTRACT: Recent evidence suggests that ADP-ribosylation factor-like tumor suppressor gene 1(ARLTS1) may act as a tumor suppressor gene. However, its role in tumor chemotherapy remains unclear. The aim of this study is to investigate the effects of ARLTS1 gene in regulation of chemosensitivity in ovarian cystadenocarcinoma cell line SKOV3.
We stably expressed wild-type (wt) ARLTS1 and empty vector (neo) in SKOV3 cells. Chemosensitivity test was carried out with four chemotherapeutic agents. Cell proliferation, cycle kinetics and apoptosis were evaluated by MTT assay and flow cytometry. Apoptosis-related proteins caspase-3 and bcl-2 were determined by Western blot analysis.
The proliferation of wtARLTS1 clones was more dramatically inhibited by all the cytotoxic agents than parental cells (P < 0.05). Increased sensitivity to chemotherapy by two to threefolds was detected in wtARLTS1 cells. The rate of apoptosis in wtARLTS1 was 60.2% treated with DDP (10× peak plasma concentration, PPC), which was dramatically higher than that of neo and parental cells (P is 0.017 and 0.020, respectively). Expression of caspase-3 and bcl-2 in parental cells declined modestly when treated with DDP, while in wtARLTS1 clones the expression of caspase-3 and bcl-2 levels declined more dramatically and become undetectable at lower DDP doses (P = 0.023 and <0.001, respectively).
Our findings suggested that ARLTS1 may facilitate chemosensitivity in ovarian cancer cells by acting synergistic with chemotherapeutic agents to induce the apoptosis signaling pathway and regulate apoptosis-related proteins.
Archives of Gynecology 12/2010; 284(5):1241-6. · 0.91 Impact Factor
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ABSTRACT: To compare the efficiency and accuracy of electroencephalographic nonlinear index (ENI) and bispectral index (BIS) in monitoring depth of anesthesia during general anesthesia.
Thirty patients undergoing elective abdominal surgery were recruited. The ability of ENI and BIS to distinguish between the anesthetic states (awake, loss of eyelash reflex, loss of response, steady anesthesia, first reaction and extubation) was analyzed with prediction probability (Pk). Correlation between ENI and BIS was tested by nonparametric Spearman rank correlation analysis, with data paired from awake through to extubation. The clinical performances of ENI and BIS were evaluated with success rate, responsiveness, and recovery time.
ENI and BIS were better than mean arterial pressure (MAP) and heart rate (HR) for distinguishing between the states of anesthesia. ENI correlated strongly with BIS from the stage of awake through to extubation (r = 0.93). No differences were found between ENI and BIS in success rate and responsiveness; but the recovery time was shorter with ENI than with BIS.
ENI and BIS provide similar information reflecting the states of anesthesia, which allows accurate distinguishment between different states of anesthesia. The clinical performance of ENI is better than that of BIS.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 01/2010; 41(1):140-4.
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ABSTRACT: To evaluate the impact of ARLTS1 gene mutations among Chinese Han-nationality patients with early-onset epithelial ovarian carcinoma (OC) in Southern China.
Tumor and blood samples collected from early-onset, late-onset epithelial ovarian carcinoma patients and control group were collected. After purification of DNA, the ARLTS1 gene fragments were amplified with PCR. The DHPLC was applied to analyze the PCR products. The mutation products were confirmed with DNA sequencing analysis.
ARLTS1 Cys148Arg revealed a significant association with an increased risk in early-onset OC group compared with both late-onset cases and controls. ARLTS1 Ser99Ser revealed no impact on both early-onset OC group and late-onset cases.
A significantly increased risk for young ovarian cancer patients with ARLTS1 Cys148Arg variant were observed, which indicate that ARLTS1 may play a important role in early-onset of OC.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 11/2009; 40(6):978-82.
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ABSTRACT: ARLTS1 has been identified in chromosome 13q14 as a tumor suppressor gene of the adenosine diphosphate-ribosylation factor family with pro-apoptotic characteristics. The ARLTS1 mutation Trp149Stop and Cys148Arg have been shown to be associated with familial cancers, but limited information is available regarding the impact of ARLTS1 variants on familial ovarian cancer (OC). The aim of this study was to evaluate the ARLTS1 genetic variants associated with familial OC risk in China. We genotyped 85 OC patients with family ovarian/breast history, 80 sporadic OC patients, and 120 controls from general population by denaturing high-performance liquid chromatography screening analysis followed by direct sequencing of the conspicuous polymerase chain reaction products. ARLTS1 Cys148Arg revealed a significant association with an increased risk of familial OC compared with both sporadic cases and controls in a dose-dependent manner (P = 0.0031 and 0.012, respectively). In the clinical-pathological study, our results support previous data in demonstrating that familial OC was associated with younger age at diagnosis (49.7 years vs 53.3 years; P = 0.014), higher proportion of tumors of advanced stages (81.2% vs 67.5%; P = 0.033), and higher rates of serous adenocarcinomas (76.4% vs 53.8%; P = 0.028) compared with sporadic OC cases. To investigate the association between genetic variants of ARLTS1 and the clinical-pathological characteristics of familial OC, we identified a significantly higher proportion of serous adenocarcinoma (55/67, 82.1%) and higher rates of advanced stage tumors (88.1% vs 55.6%; P = 0.004) in ARLTS1 Cys148Arg carriers. We showed a significantly increased risk of familial OC for ARLTS1 Cys148Arg variant, which indicate that ARLTS1 may play a role in familial OC.
International Journal of Gynecological Cancer 06/2009; 19(4):585-90. · 1.65 Impact Factor
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ABSTRACT: Removal of a tracheal or bronchial foreign body is a common emergent surgical procedure in children. The anesthetic management can be challenging. EMLA Cream (EC) has been widely used to provide topical anesthesia. In the present study, we evaluate the efficacy and safety of EC coated on the rigid bronchoscope for tracheobronchial foreign body removal in children undergoing intravenous anesthesia with spontaneous ventilation.
The authors conducted a randomized, double-blind, placebo-controlled clinical trial.
Thirty patients were randomized to receive either EC or placebo (lubricant ointment) coated on the rigid bronchoscope. Intravenous anesthesia and spontaneous ventilation were performed in all patients. Heart rate, blood pressure, pulse oxygen saturation (SpO(2)) and frequency and degree of breath holding were recorded. After surgery, the bronchoscopist rated overall surgical manipulation as excellent, fair, and poor. The durations of postoperative care were also recorded.
Episodes of oxygen desaturation (SpO(2) < 90%) occurred in 3/15 (20%) patients in the EC group and in 9/15 (60%) patients in the control group (P < .05). Occurrences and degrees of breath holding were less in the EC group than that in the control group (P < .05). Ranks of surgical manipulation were excellent in 80% of patients in the EC group versus 13% of patients in the control group (P < .05). The durations of postoperative care were shorter in the EC group than that in the control group (P < .05).
EC coated on the rigid bronchoscope combined with intravenous anesthesia could provide more efficacious and safer anesthesia for tracheobronchial foreign body removal in children under spontaneous ventilation.
The Laryngoscope 01/2009; 119(1):158-61. · 1.75 Impact Factor
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ABSTRACT: To investigate the effect of ARLTS1 (ADP-ribosylation factor-like tumor suppressor gene 1) on the growth and apoptosis of ovarian cystadenocarcinoma cell line SKOV3.
The recombinant plasmid pCMV-Tag 3B-ARLTS1 was constructed and transfected into SKOV3 cells by liposome protocol. The expression of ARLTS1 mRNA and its protein were examined by RT-PCR and Western blotting. Effects of ARLTS1 gene on growth and apoptosis of the transfected cells were evaluated by MTT assay and flow cytometry (FCM). The alterations of caspase-3 and bcl-2 protein levels were examined by Western blotting.
The stable transfection of pCMV-Tag 3B-ARLTS1 in SKOV3 cells were obtained and verified by RT-PCR and Western blotting methods. After transfected with ARLTS1, more SKOV3 cells were observed in S phase by FCM compared with those in the other two groups (P is 0.035 and 0.011, respectively). The 36.7% apoptotic index of ARLTS1 transfected cell was significantly higher than that of the other two groups (P < 0.001). The growth of ARLTS1 transfected cells was dramatically inhibited compared with cells transfected with Vector (P < 0.05). Western blotting indicated a significantly decrease in caspase-3 and bcl-2 protein levels in cells transfected with ARLTS1 compared with SKOV3 cells (P is 0.021 and 0.013, respectively).
ARLTS1 transfection can inhibit proliferation and induce apoptosis of SKOV3 cells in vitro and down-regulate the expression of caspase-3 and bcl-2. ARLTS1 gene may be a candidate tumor suppressor gene in ovarian cancer.
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 01/2009; 40(1):6-10.
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ABSTRACT: The purpose of this study was to investigate the expression patterns and prognostic impact of estrogen receptor (ER), progesterone receptor (PR) and their combinations in young patients with ovarian carcinomas (OC) in China.
We collected data on 86 patients diagnosed of OC younger than or equal to 40 years of age with a long clinical follow-up. Immunohistochemical staining was performed to assess ER and PR expression in paraffin-embedded ovarian cancers. Samples in which more than 10% of cells showed stained nuclei were interpreted as positive for the particular receptor. Survival rates (Kaplan-Meier method) were compared by the log rank test. A multivariate analysis (Cox proportional hazards) was used to determine the independent effect of each variable on survival.
ER were found positive in 64.0% of all cases but most highly expressed in serous types (P=0.047). PR-positive expression accounted for 57.0% of all cases and elevated in early FIGO stages and well-differentiated diseases (P=0.004 and 0.012, respectively). The expression of PR, but not ER, was associated with favorable survival in Kaplan-Meier survival analyses (P<0.001). The ER+PR+ combination, which was associated with low FIGO stages (P=0.012) and low tumor grade (P=0.046), showed a superior course of patients' overall survival compared with all other combinations (5-year survival rate 95.0% vs. 73.4%, log rank P=0.001). PR status (hazard ratio 1.93, P<0.001) together with FIGO stage (hazard ratio 2.19, P<0.001) were proved to be independent prognostic factors by multivariate analysis.
This study indicates that the expression of PR, especially the ER+PR+ phenotype predicts a favorable clinical outcome for young patients of OC.
Gynecologic Oncology 01/2009; 113(1):99-104. · 3.89 Impact Factor
