A Amorosi

University of Catania, Catania, Sicily, Italy

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Publications (129)413.15 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A 34-year-old female was referred to us for a consultation of her dermatological lesions (pigmented lesions present in her oral cavity and on her right shoulder) in May 2007. These lesions had been present since childhood. Recently, the patient had developed seizures and a headache. An MRI of the brain showed the presence of two intracranial masses. The intracranial tumours were surgically removed whereas skin and mucosal lesions were biopsied. Histological findings of brain tumours were consistent with a diagnosis of “melanocytoma” while cutaneous lesions presented “benign dermal melanocytic infiltrations”. Whole brain irradiation was performed. After 3 months a new melanocytic skin lesion appeared on the scalp with histological picture similar to the other cutaneous ones. At the 5-year follow-up examination no recurrence of intracranial tumour or other skin or mucosal lesions were registered. According to the clinical and histological findings, we classify our case as a form of neurocutaneous melanosis in a young adult patient and we present it for the rarity of this syndrome, for the difficulty of the diagnosis, for the potential aggressive behaviour of intracranial lesions that necessitates a constant attentive follow-up and for the unusual feature of new developing skin lesion during the course of the disease.
    European Journal of Inflammation 01/2013; 11(3):871-876. · 5.23 Impact Factor
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    ABSTRACT: La leishmaniosi è una zoonosi mediterranea, causata da protozoi del genere Leishmania, trasmessa da insetti ematofagi. La letteratura della leishmaniosi riporta casi di localizzazione laringea primitiva e di infezione secondaria a riattivazione di leishmaniosi cutanea o muco-cutanea, in pazienti immunodepressi o trattati con corticosteroidi inalatori. Un uomo di 51 anni è giunto alla nostra osservazione riferendo disfonia ingravescente da quattro anni. La laringoscopia ha evidenziato una lesione vegetante sulla corda vocale di sinistra, che è stata ritenuta sospetta per precancerosi. L’esame istologico ha rivelato la presenza di displasia lieve. Il paziente è stato sottoposto a controlli laringoscopici mensili. Una seconda biopsia è stata eseguita dopo tre mesi, per il peggiorare della disfonia e l’evidenza di una nuova lesione leucoplasica. L’esame istologico ha identificato la presenza di Leishmania Infantum. Il paziente è stato inviato presso il reparto di Malattie Infettive, dove sono state identificate tre lesioni cutanee e splenomegalia, ed è stata formulata la diagnosi di leishmaniosi muco-cutanea. Il paziente è stato trattato con Amfotericina B e.v. ed ha raggiunto una remissione quasi completa. Nel nostro caso la displasia laringea ha comportato una precisa sorveglianza endoscopica della laringe, che ha consentito di identificare precocemente la seconda lesione. Questa lesione è stata considerata un’evoluzione della precancerosi e solo l’esame istologico ha identificato la localizzazione laringea della Leishmania Infantum. Questo è il primo caso di leishmaniosi insorgente su displasia e sebbene la localizzazione laringea non fosse primitiva, i sintomi disfonici hanno condotto all’identificazione della leishmaniosi ed al trattamento appropriato con guarigione del paziente.
    Gazzetta medica italiana 12/2012; 171(6):823-7.
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    ABSTRACT: We describe a case of brain abscesses in a cirrhotic and diabetic 57-year-old woman showing fever, aphasia, right hemiparesis and seizures. Neuroradiological investigation revealed unilateral cerebritis evolving in multiple abscesses. From blood and surgical drainage samples Listeria monocytogenes grew in pure culture. Despite decompressive craniotomy, the patient died two months after hospital admission.
    Le infezioni in medicina: rivista periodica di eziologia, epidemiologia, diagnostica, clinica e terapia delle patologie infettive 12/2012; 20(4):279-83.
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    ABSTRACT: Literature data indicate that mast cells (MCs) are involved in angiogenesis through the release of several pro-angiogenetic factors among which tryptase, a serine protease stored in MC granules, is one of the most active. However, no data are available concerning the role of MCs during keloids' angiogenesis. In this study, we evaluated the correlations of the number of MCs positive to tryptase (MCDPT) and microvascular density (MVD) within a series of 15 keloids and 10 normotrophic scars, by means of immunohistochemistry and image analysis methods. Data demonstrated a significant difference of MVD and MCDPT between keloids and normotrophic scars and a significant correlation between MVD and MCDPT in keloids. Our results suggest that tryptase-positive MCs might play a key role in keloids' angiogenesis. In this context, several tryptase inhibitors might be clinically evaluated as a possible new anti-angiogenetic approach to prevent keloid formation after surgery.
    Updates in surgery. 11/2012;
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    ABSTRACT: BACKGROUND: The clinical evolution of laryngeal squamous cell carcinoma (SCC) is undetectable with the current staging criteria. To more completely understand the biology of laryngeal SCC, we assessed the expression of the proteins B-cell-specific Moloney murine leukemia virus integration site 1 (BMI1) and p16. METHODS: We assessed immunohistochemically the expression of BMI1 and p16 in 25 laryngeal SCCs at different stages. RESULTS: High BMI1 expression was detected in 11.7% of glottic tumors and in 50% of supraglottic tumors. No significant differences were observed in the patients' clinical data after they were stratified by the tumor expression of p16. The expression of nuclear BMI1 in the absence of p16 immunoreactivity correlated significantly with the pN status of the primary tumors. CONCLUSION: Nuclear BMI1 expression in the absence of p16 expression seems to characterize a subset of patients with a high risk of developing lymph node metastasis. © 2012 Wiley Periodicals, Inc. Head Neck, 2012.
    Head & Neck 06/2012; · 2.83 Impact Factor
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    ABSTRACT: Calcific aortic stenosis is a progressive disease characterized by massive fibrosis andmineralization of the valve leaflets. The aim of this study was to determine whether the onset of native calcific aortic stenosis is associated primarily with matrix remodelling events, and particularly with elastin degradation. The immunohistochemical expression profile of matrix degradating enzymes and tenascin-C was investigated in both healthy and native calcified aortic valves. Collagen and elastic tissue were studied by light microscopy and electron microscopy. Immunophenotypic analysis of inflammatory cells was carried out by using monoclonal antibodies to macrophages, T and B lymphocytes. Immunoreactivity for tenascin-C and matrix metalloproteinase-12 (MMP-12) was associated with areas of dense mineralization, which were characterized by fibrosis, fragmentation and calcification of elastic fibres a positive reaction was also found around small islands of calcification. MMP-11 was not detected in the diseased valves. Osteopontin and osteonectin were also found at sites of mineralization. All calcified valves examined showed inflammatory cell infiltration. Our results demonstrate the direct involvement of MMP-12 in native aortic valve stenosis. MMP-mediated degradation of elastic fibres might contribute actively to valve mineralization by inducing calcium deposition onto fragmented elastin.
    Histopathology 09/2011; 59(3):504-13. · 2.86 Impact Factor
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    ABSTRACT: Highlights ► Vaginal AA is potentially a sex hormone dependent tumor. ► GnRH-a plus raloxifene is an effective neoadjuvant and adjuvant therapy for AA.
    Gynecologic Oncology 07/2011; 123(1):172-3. · 3.93 Impact Factor
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    Leukemia: official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 01/2011; 25(4):707-11. · 10.16 Impact Factor
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    ABSTRACT: Selective cyclooxygenase-2 (COX-2) inhibitors and proton pump inhibitors may exert immune-mediated effects in human gastric mucosa. T-cell immune response plays a role in Helicobacter pylori-induced pathogenesis. This study evaluated effects of celecoxib and lansoprazole on T-helper (Th) 1 and Th2 immune response in human gastric mucosa. Dyspeptic patients with or without osteoarticular pain were given one of the following 4-week therapies: celecoxib 200 mg, celecoxib 200 mg plus lansoprazole 30 mg, and lansoprazole 30 mg daily. Expression of COX-2, T-bet, and pSTAT6 and production of prostaglandin E₂ (PGE₂), interferon (IFN)-γ, and interleukin (IL)-4 were determined in gastric biopsies before and after therapy. Histology was evaluated. Cyclooxygenase-2 expression and PGE₂ production was higher, and Th1 signaling pathway was predominant in H. pylori-infected vs. uninfected patients. T-bet expression and IFN-γ production increased, while STAT6 activation and IL-4 production decreased following therapy with celecoxib and celecoxib plus lansoprazole, respectively. Th1 and Th2 signaling pathways down-regulated after therapy with lansoprazole, and this was associated with an improvement of gastritis. Effect of therapy was not affected by H. pylori status. Celecoxib and lansoprazole modulate Th1/Th2 immune response in human gastric mucosa. The use of these drugs may interfere with long-term course of gastritis.
    Helicobacter 10/2010; 15(5):449-59. · 3.51 Impact Factor
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    ABSTRACT: Osteonecrosis of the jaw is a severe bone disorder traditionally associated with periodontal disease, local malignancy, chemotherapy, glucocorticoid therapy, or trauma. Recently a growing number of publications reported the occurrence of osteonecrosis of the jaw in patients undergoing treatment with bisphosphonates. The mechanism by which bisphosphonates might contribute to the development of osteonecrosis of the jaw is far from being fully elucidated. Suppression of bone turnover, infection, tissue hypoxia and cellular toxicity were proposed as possible mechanisms by which bisphosphonates may exert adverse effects on bone metabolism. Here, we studied 25 consecutive patients treated with bisphosphonates for osteoporosis or tumoral pathologies. We provide good evidence of hyperactive osteoclastic bone resorption and suggest a direct cytotoxic effect of bisphosphonates on bone tissue through induction of osteocyte cell death. We also demonstrate that bisphosphonates only have limited adverse effects on bone vascular network.
    Ultrastructural Pathology 08/2010; 34(4):207-13. · 0.98 Impact Factor
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    ABSTRACT: Recent studies suggest that in patients with carpal tunnel syndrome, pathological changes occur in the subsynovial connective tissue. Such changes are non-inflammatory synovial fibrosis and vascular proliferation. Thickening of the tendon sheet may cause an increase of canal pressure and damages to the median nerve in the wrist; however, the causes of such events still remain to be clarified. We examined synovial specimens from 26 patients operated on for idiopathic carpal tunnel syndrome. Analysis included histological, ultrastructural and immunohistochemical examination in order to establish a pathological underlying pattern. An explanation for the pathogenesis of the found changes suggested. Our data confirm the presence of a non-inflammatory fibrosis with irregular bundles of collagen. De novo blood vessel formation was also noted. Interestingly the neo-angiogenesis consists of anomalous vessels and may be triggered from various cell types secreting vascular endothelial growth factor (VEGF), including macrophage-like elements similar to endothelial progenitor cells. Therefore, we believe that in the future a non-surgical management of carpal tunnel syndrome might be conjecturable via anti-VEGF drugs.
    Clinical neuropathology 01/2009; 28(2):129-35. · 1.34 Impact Factor
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    ABSTRACT: Gastric biopsies obtained through endoscopy from patients uninfected by Helicobacter pylori were co-cultured with an H. pylori strain. According to tissue and H. pylori viability, interleukin 8 was increased in biopsy homogenate and supernatant after 12-36h culture. This simple method is suitable to investigate early phases of bacteria-host interaction.
    Journal of Microbiological Methods 07/2008; 75(2):346-9. · 2.16 Impact Factor
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    ABSTRACT: Myxomas are the most frequent primary cardiac neoplasms. They have an abundant extracellular matrix rich in proteoglycans. Interactions between cells and matrix are very important in the development of tumors, but data about myxomas in this setting are scarce because of the rarity of such neoplasms. The expression of tenascin-c and hyaluran receptors in cardiac myxoma has never been investigated. Moreover, it is now well recognized that cells of cardiac myxoma differentiate along endothelial lines. We have analyzed left atrial myxomas from 13 consecutive patients (six male and seven female, surgically treated), via immunohistochemical methods for the expression of molecules also implicated in angiogenesis in normal and pathological conditions, like tenascin-c and hyaluran receptors CD44s, CD44v5 and CD44v6. Our data suggest that tenascin-c and CD44s play a synergic and perhaps complementary role in development of cardiac myxomas. In particular, tenascin-c seems to promote aggregation of cells and differentiation in vascular structures, whereas CD44s receptors might be important for cellular motility. Cell proliferation rate in such tumors was very low (MIB-1 labeling index <1%) and uniform in all the areas of the neoplasms regardless of the presence of characteristic structures such as cords and rings of multinucleated cells or the expression of tenascin-c and CD44 receptors. This study shows that cardiac myxomas express in the extracellular matrix tenascin-c and on the cellular membranes of neoplastic cells the hyaluran receptor CD44s. Such molecules take part in the mechanism of development of the myxomas and might be in the future the target of nonsurgical treatments.
    Cardiovascular pathology: the official journal of the Society for Cardiovascular Pathology 05/2008; 18(3):173-7. · 1.63 Impact Factor
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    ABSTRACT: Previous studies suggest the expression of UbcH10 gene, that codes for a protein belonging to the ubiquitin-conjugating enzyme family, as a valid indicator of the proliferative and aggressive status of tumors of different origin. Therefore, to look for possible tools to be used as diagnostic markers in astrocytic neoplasias, we investigated UbcH10 expression in normal brain, gliosis and low-grade and high-grade astrocytic tumors by immunohistochemistry. UbcH10 expression was observed in low-grade astrocytoma and in glioblastoma. Our data indicate a clear correlation between UbcH10 expression and the histological grade of the astrocytic tumors. Moreover, the analysis of UbcH10 expression allows the differentiation between gliotic and malignant tissues. Finally, since proteasome inhibitors have recently been considered as possible drugs in the chemotherapy of various tumors, our results would suggest new perspectives for the treatment of brain malignancies based on the suppression of the UbcH10 function.
    Clinical neuropathology 01/2008; 27(4):219-23. · 1.34 Impact Factor
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    ABSTRACT: Ultrastructural data about large cell variant ovarian small cell carcinoma (LCV-SCC) are scarce and contradictory and the role of transmission electronmicroscopy (TEM) is not clear in the assessment of such tumors. The authors present a case of LCV-SCC without hypercalcemia in a 30-year-old woman. The diagnosis was confirmed by histopathological and immunohistochemical studies. Cytopathological examination of peritoneal washing showed a population of large neoplastic cells. TEM demonstrated that the neoplasia comprised two types of cells: one type showed many coarse secretory granules without dense core, and the other type was without granules and showed dilated endoplasmic reticulum and sometimes indented nuclei. The present case indicates that different underlying ultrastructural patterns, not yet well known, exist in connection with the pathological and clinical behaviour of LCV-SCC. TEM might play a role in the identification of subtypes of LCV-SCC with different prognostic and therapeutic impact.
    Ultrastructural Pathology 01/2008; 32(5):206-10. · 0.98 Impact Factor
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    ABSTRACT: Animal experiments using pharmacological agents acting on the dopaminergic system, such as apomorphine, have been used as suitable models of schizophrenia, based on the dopaminergic hypothesis of this disorder. To determine whether dopaminergic hyperactivity may produce neuropathological changes, young Mongolian gerbils were treated with apomorphine (0.45 mg/kg) and the hippocampal CA1 region was subsequently studied by transmission and scanning electron microscopy. Acute subcutaneous administration of apomorphine induced pronounced degenerative changes in hippocampal neurons, such as swollen dendrites and axons in the neuropil and swelling of synaptic endings with a decrease in the number of synaptic vesicles. In conclusion, we think that this animal model may provide important indications about a possible dopaminergic hyperactivation mechanism, that could produce pathological changes in the hippocampus similar to those encountered in psychotic patients.
    Schizophrenia Research 10/2007; 95(1-3):223-7. · 4.59 Impact Factor
  • International Journal of Surgical Pathology 11/2006; 14(4):332-3. · 0.76 Impact Factor
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    ABSTRACT: Gli studi precedenti del nostro gruppo suggeriscono che l’espressione del gene UbcH10, che codifica per la proteina che appartiene alla famiglia degli enzimi ubiquitina-coniugasi, è un indicatore valido della proliferazione e dell’aggressività dei carcinomi di varia origine. Di conseguenza, per cercare nuove metodiche da usare come indicatori diagnostici per le neoplasie astrocitarie, abbiamo studiato l’espressione UbcH10 nel cervello normale, nella gliosi e nei tumori astrocitari di basso ed alto grado mediante tecniche di immunoistochimica. Materiali e metodi. Abbiamo studiato: tumori da 13 pazienti operati; 3 cervelli normali da autopsia; gliosi da 4 cervelli dei pazienti operati per epilessia. L’espressione dell’UbcH10 è stata determinata mediante tecniche di colorazione immunoistochimica. L’indice di proliferazione è stato valutato usando l’anticorpo MIB-1. Un’analisi semiquantitativa è stata eseguita mediante una valutazione della percentuale dei nuclei colorati e dell’intensità di colorazione. Risultati. L’espressione UbcH10 è stata osservata negli astrocitomi di basso grado e nei glioblastomi. Il punteggio totale nei Glioblastomi era il doppio rispetto agli astrocitomi. Il cervello normale ed il cervello con gliosi non hanno mostrato colorazioni per la proteina UbcH10. Discussione. I nostri dati suggeriscono che la sovraespressione dell’enzima UbcH10 può giocare un ruolo nel meccanismo di genesi dei tumori astrocitici e si può correlare con il grado della neoplasia. Inoltre, l’analisi dell’espressione UbcH10 permette la differenziazione fra i tessuti gliotic e maligni. Per concludere, poiché gli inibitori dei proteosomi, recentemente, sono stati considerati come farmaci da poter utilizzare nella chemioterapia di vari tumori, i nostri risultati indicherebbero nuovo prospettive per il trattamento delle neoplasie maligne cerebrali sfruttando la soppressione della funzione delle UbcH10 mediante questi farmaci.
    XI Congresso Nazionale Associazione Italiana di Neuro Oncologia, Bergamo; 10/2006
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    ABSTRACT: To evaluate the prevalence of Giardia lamblia (G. lamblia) infection in patients with irritable bowel syndrome (IBS) and dyspepsia and to establish which is the most accurate test to diagnose the infection in this setting. One hundred and thirty-seven patients who consecutively attended the Outpatient Gastroenterology Clinic for the first time between January 2002 and December 2003 due to symptoms of IBS and/or dyspepsia were recruited. All patients underwent clinical evaluation, first-step haematology and chemistry tests, serologic assays for celiac disease, lactose-H(2) breath test, abdominal ultrasonography, and esophagogastroduodenoscopy. Helicobacter pylori status was evaluated. In patients with symptoms of IBS older than 45 years, colonoscopy was also performed. In all patients, duodenal biopsies and stool samples were examined for trophozoites and cysts of G. lamblia by several methods. G. lamblia was identified in 9 patients. The following diagnoses were also made: IBS (100/137, 73%), functional dyspepsia (62/137, 45%), organic dyspepsia (33/137, 24%), and lactose intolerance (75/137, 55%). A significant association was found between giardiasis and H pylori infection (c2=6.632, OR=12.4, CI=1.5-68.1). There were no symptoms that reliably allowed the recognition of giardiasis. Direct search of the parasite in duodenal biopsy and stool sample examinations gave concordant results in all cases while histological examination of duodenal biopsies displayed a low sensitivity (e.g., 22.2%). In this consecutive series, diagnosis of G. lamblia infection accounted for 6.5% of patients with IBS and dyspepsia. Duodenal biopsies for diagnosis of giardiasis may be unnecessary if stool sample examination is performed.
    World Journal of Gastroenterology 04/2006; 12(12):1941-4. · 2.55 Impact Factor
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    ABSTRACT: Eccrine porocarcinomas (EP) are skin appendage tumors originating from the acrosyringium. Pagetoid form is rare and exceptionally it can involve eyelid. We report a 70-year-old patient presenting a lesion sited on the left cheek region involving the internal canthus, the dorsum of nose and the half inferior eyelid in full thickness. A wide surgical excision was performed and after 2 years of follow up no recurrences have been seen.
    Acta chirurgiae plasticae 02/2006; 48(4):115-8.

Publication Stats

2k Citations
413.15 Total Impact Points

Institutions

  • 2012
    • University of Catania
      Catania, Sicily, Italy
  • 2000–2010
    • Universita' degli Studi "Magna Græcia" di Catanzaro
      • Department of Health Sciences
      Catanzaro, Calabria, Italy
    • National Institutes of Health
      • Laboratory of Human Carcinogenesis
      Bethesda, MD, United States
  • 2006
    • Università della Calabria
      Rende, Calabria, Italy
  • 1993–2002
    • University of Florence
      • • Dipartimento di Scienze Biomediche, Sperimentali e Cliniche
      • • Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino
      Florence, Tuscany, Italy
  • 1998
    • NCI-Frederick
      Maryland, United States
  • 1997
    • Istituto Toscano Tumori
      Florens, Tuscany, Italy
    • University Medical Center Hamburg - Eppendorf
      Hamburg, Hamburg, Germany
  • 1995
    • Leidos Biomedical Research
      Maryland, United States
  • 1992
    • Università di Pisa
      • Department of Clinical and Experimental Medicine
      Pisa, Tuscany, Italy