Kazuhiro Shiraishi

Publications (7)41.09 Total impact

• Source

  Available from: pnas.org

  Article: A neurosphere-derived factor, cystatin C, supports differentiation of ES cells into neural stem cells.

  Takeo Kato, Toshio Heike, Katsuya Okawa, Munetada Haruyama, Kazuhiro Shiraishi, Momoko Yoshimoto, Masako Nagato, Minoru Shibata, Tomohiro Kumada, Yasunari Yamanaka, Haruo Hattori, Tatsutoshi Nakahata
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  ABSTRACT: Although embryonic stem (ES) cells are capable of unlimited proliferation and pluripotent differentiation, effective preparation of neural stem cells from ES cells are not achieved. Here, we have directly generated under the coculture with dissociated primary neurosphere cells in serum-free medium and the same effect was observed when ES cells were cultured with conditioned medium of primary neurosphere culture (CMPNC). ES-neural stem cells (NSCs) could proliferate for more than seven times and differentiate into neurons, astrocytes, and oligodendrocytes in vitro and in vivo. The responsible molecule in CMPNC was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, which turned out to be cystatin C. Purified cystatin C in place of the CMPNC could generate ES-NSCs efficiently with self-renewal and multidifferentiation potentials. These results reveal the validity of cystatin C for generating NSCs from ES cells.
  Proceedings of the National Academy of Sciences 05/2006; 103(15):6019-24. · 9.68 Impact Factor
• Article: [Postoperative complete atrioventricular block induced by carbamazepine in a patient with congenital heart disease].

  Tomohiro Kumada, Haruo Hattori, Hiraku Doi, Kazuhiro Shiraishi, Yasunari Yamanaka, Takeo Kato, Tatsutoshi Nakahata
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  ABSTRACT: We report here a boy with epilepsy and congenital heart defect, complicated postoperatively by complete atrioventricular (A-V) block caused by an adverse effect of carbamazepine (CBZ). He had been taking CBZ for 7 years to treat complex partial seizures. He also had endocardial cushion defect and first-degree A-V block, and underwent cardiac surgery at the age of 17 years. The postoperative course was unremarkable except transient complete left bundle branch block occuring one day after the surgery. Oral CBZ (400 mg per day) was continued. Five days after the surgery, bradycardia (20 beats per minute) suddenly developed, and electrocardiography (ECG) showed complete A-V block. Pervenous pacing was begun, and the heart rate gradually recovered. CBZ was discontinued on the suspicion that it caused the arrhythmia, although its serum level was estimated to be within the therapeutic range (4 to 5 microg/ml). He underwent pervenous pacing for 12 days. He was discharged 27 days after the surgery, when ECG returned to first-degree A-V block. In this case, the cardiac conduction system was affected by an adverse effect of CBZ, in combination with the preoperative first-degree A-V block and the effects of cardiac surgery, resulting in complete A-V block. Although reports of similar cases are scarce, caution should be made in prescribing CBZ to patients who either have cardiac conduction abnormalities or undergo cardiac surgery.
  No to hattatsu. Brain and development 06/2005; 37(3):257-61.
• Article: Clinical course and prognosis of 27 patients with childhood onset multiple sclerosis in Japan.

  Kazuhiro Shiraishi, Yoshihisa Higuchi, Kyoko Ozawa, Qi Hao, Takahiko Saida
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  ABSTRACT: To investigate whether outcomes of childhood onset multiple sclerosis (MS) have changed or not, the clinical courses of childhood onset MS in 27 patients at our hospital and those reported by Fukuyama in 1991 were compared. The ratios of our patients with poor prognosis in walking and vision were decreased. Widespread use of high-dose corticosteroid therapy and interferon therapy may be one cause of the low frequency of severe sequelae in our study.
  Brain and Development 05/2005; 27(3):224-7. · 2.12 Impact Factor
• Article: Dystonia in a 13-year-old boy with secondary progressive multiple sclerosis.

  Kazuhiro Shiraishi, Yoshihisa Higuchi, Kyoko Ozawa
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  ABSTRACT: We report a patient who developed relapsing-remitting multiple sclerosis (MS) at 8 years old, and then had a progressive clinical course and dystonia. Dystonia of the patient is probably due to a lesion of the basal ganglia. Abnormal posture or movement disorder is very rarely found in MS, and progressive clinical course is also rare in childhood. The patient is worthy of attention because of his childhood onset, progressive clinical course and dystonia.
  Brain and Development 01/2005; 26(8):539-41. · 2.12 Impact Factor
• Article: Myelination of a fetus with Pelizaeus-Merzbacher disease: immunopathological study.

  Kazuhiro Shiraishi, Masayuki Itoh, Kenji Sano, Sachio Takashima, Takeo Kubota
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  ABSTRACT: We report an autopsied case of a 21-gestational-week fetus with duplication of the proteolipid protein (PLP) gene (PLP1). An immunohistochemical study, which can detect the specific expression of PLP, myelin basic protein, myelin-associated glycoprotein, and platelet-derived growth factor receptor alpha subunit in brain tissues, showed that the myelination was almost the same as that of age-matched controls. This result suggests that the development and migration of the oligodendrocyte is normal in Pelizaeus-Merzbacher disease until midgestation. To our knowledge, this is the first report of the myelination of a fetus with duplication of the PLP1 gene.
  Annals of Neurology 09/2003; 54(2):259-62. · 11.09 Impact Factor
• Article: Cyclosporin A treatment for membranoproliferative glomerulonephritis type II.

  Takahiro Kiyomasu, Minoru Shibata, Hideo Kurosu, Kazuhiro Shiraishi, Hisako Hashimoto, Tadashi Hayashidera, Yuichi Akiyama, Nobuaki Takeda
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  ABSTRACT: A nephrotic patient with membranoproliferative glomerulonephritis type II (MPGN II) was treated with cyclosporin A (CSA) and alternate-day low-dose prednisolone. This patient developed the nephrotic syndrome twice. The second episode of the nephrotic syndrome was steroid resistant, and therefore this patient was treated with a CSA regimen. During treatments with alternate-day low-dose prednisolone and CSA, this patient recovered from the nephrotic syndrome. We conclude that CSA therapy may be effective for patients with the steroid-resistant nephrotic syndrome caused by MPGN II.
  Nephron 08/2002; 91(3):509-11. · 13.26 Impact Factor
• Article: Loss of Borealin/DasraB leads to defective cell proliferation, p53 accumulation and early embryonic lethality.

  Yasunari Yamanaka, Toshio Heike, Tomohiro Kumada, Minoru Shibata, Yuki Takaoka, Ayumi Kitano, Kazuhiro Shiraishi, Takeo Kato, Masako Nagato, Katsuya Okawa, Kenryo Furushima, Kazuki Nakao, Yukio Nakamura, Makoto Mark Taketo, Shinichi Aizawa, Tatsutoshi Nakahata
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  ABSTRACT: Borealin/DasraB is a member of the chromosomal passenger protein complex (CPC) required for proper segregation of chromosomes during mitosis. In Drosophila melanogaster, inactivation of Borealin/DasraB results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. However, the in vivo role of mammalian Borealin/DasraB remains unclear. Here, we analyzed the expression of Borealin/DasraB and found that borealin is widely expressed in embryonic tissues and later restricted to adult tissues which relies on rapid cell proliferation. To determine the role of borealin during mouse development, we generated borealin-null mice through targeted disruption. While heterozygous mice developed normally, disruption of both borealin alleles resulted in early embryonic lethality by 5.5 dpc (days postcoitus) due to mitotic defects and apoptosis in blastocyst cells that showed microtubule disorganization and no CPC enrichment. At 5.5 dpc, borealin-null embryos exhibited excessive apoptosis and elevated expression of p53. However, loss of p53 did not abrogate or delay embryonic lethality, revealing that Borealin/DasraB inactivation triggered impaired mitosis and apoptosis though p53-independent mechanisms. Our data show that Borealin/DasraB is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53.
  Mechanisms of development 125(5-6):441-50. · 2.83 Impact Factor