Miroslawa Z Barciszewska

Publications (22)50.72 Total impact

• Source

  Available from: psychepharmaceuticals.com

  Article: Promising human brain tumors therapy with interference RNA intervention (iRNAi).

  Katarzyna Rolle, Stanislaw Nowak, Eliza Wyszko, Monika Nowak, Ryszard Zukiel, Rafal Piestrzeniewicz, Iwona Gawronska, Miroslawa Z Barciszewska, Jan Barciszewski
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  ABSTRACT: Glioblastoma multiforme (GBM) is the most common type of malignant gliomas, characterized by genetic instability, intratumoral histopathological variability and unpredictable clinical behavior. Disappointing results in the treatment of gliomas with surgery, radiation and chemotherapy have fuelled a search for new treatment modalities. Malignant gliomas express preferentially a number of surface markers that may be exploited as therapeutic targets, such as tenascin-C (TN-C), an extracellular matrix glycoprotein that contributes to tumor cell adhesion, invasion, migration and proliferation. In this paper we describe a novel strategy for human brain tumors therapy based on RNA interference (RNAi) and its application after surgery (intervention with RNAi) to inhibit TN-C synthesis. We present data of 46 patients suffering from brain tumors resected and treated with dsRNA with the sequence homology of tenascin-C mRNA (ATN-RNA). The specific effect of ATN-RNA on TN-C downregulation was proved with antibodies against TN-C in glioblastoma multiforme cultured cells. A significant improvement in overall survival (OS) without loosing the quality of life (QOL) of patients was observed. MRI and CT studies showed tumor growth delay or lack of tumor recurrence. This novel therapy based on RNA interference shows a hopeful therapeutical potential. To our knowledge the intervention with RNAi (iRNAi) method is the first protocol of RNAi application in human brain tumor treatment.
  Cancer biology & therapy 03/2010; 9(5):396-406. · 2.64 Impact Factor
• Article: Global DNA methylation changes in blood of patients with essential hypertension.

  Iwona Smolarek, Eliza Wyszko, Anna M Barciszewska, Stanislaw Nowak, Iwona Gawronska, Anna Jablecka, Miroslawa Z Barciszewska
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  ABSTRACT: Hypertension is a common disease of the cardiovascular system and one of the main causes of mortality in the world. Its etiopathogenesis and molecular mechanisms are unknown. Epigenetic changes may play a role in its development. Therefore the level of 5-methylcytosine (5mC), a well-known epigenetic marker, was analyzed in DNA from the blood of essential hypertension patients. TLC chromatographic analysis of the DNA nucleotide composition was used to determine 5mC levels in blood DNA samples from 60 patients suffering from essential hypertension (30 with stage 1 and 30 with stage 2 hypertension) and 30 control subjects. The mean levels of 5mC were 1.80 + or - 0.69 in the healthy subjects, 1.14 + or - 0.48 in all the patients with essential hypertension, 1.29 + or - 0.50 in those with stage 1, and 0.99 + or - 0.42 in those with stage 2 of hypertension. Statistically significant differences in 5mC amount in DNA were observed between the control group and the whole patient group, the control group and each subgroup of patients, and the groups of patients with stage 1 and stage 2 of hypertension. The level of 5mC in the DNA of the essential hypertension patients was independent of clinical and biochemical factors. The level of 5mC in the DNA of patients suffering from essential hypertension is lower than in healthy people and depends of the progression of hypertension.
  Medical science monitor: international medical journal of experimental and clinical research 02/2010; 16(3):CR149-155. · 1.70 Impact Factor
• Chapter: Interference RNA Intervention in Brain Tumors

  Katarzyna Rolle, Stanislaw Nowak, Eliza Wyszko, Monika Nowak, Ryszard Zukiel, Rafal Piestrzeniewicz, Iwona Gawronska, Miroslawa Z. Barciszewska, Jan Barciszewski
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  ABSTRACT: Malignant gliomas preferentially express a number of surface markers that may be exploited as therapeutic targets, such as tenascin-C, an extracellular matrix glycoprotein which contributes to tumor cell adhesion, invasion, migration, and proliferation. Glioblastoma multiforme (GBM) is the most common form of malignant glioma, characterized by genetic instability, intratumoral histopathological variability, and unpredictable clinical behavior. Disappointing results in the treatment of gliomas with surgery, radiation, and chemotherapy have fueled the search for new treatment modalities. In this paper, we present data from RNA interference experimental therapy of patients suffering from brain tumors. After surgical resection, they were treated with dsRNA (ATN-RNA) complementary to the part of the sequence of tenascin-C mRNA. MRI and CT follow-up studies showed a tumor growth delay or lack of its recurrence, due to inhibition of TN-C synthesis. A significant improvement in overall survival (OS) without losing the quality of life (QOL) of the patients was observed. This novel therapy intervention based on RNA interference (iRNAi) shows a big therapeutical potential and, to our knowledge, is the first application of RNAi in human disease treatment.
  08/2009: pages 221-253;
• Chapter: Diagnosis of Brain Tumors Through Global Specific DNA Methylation Analysis

  Anna-Maria Barciszewska, Stanislaw Nowak, Iwona Gawronska, Miroslawa Z. Barciszewska
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  ABSTRACT: We describe a new, simple and reliable method for diagnosis of a neoplastic disease. It is based on the cellulose thin layer chromatography (TLC) quantitative determination of 5-methylcytosine (m5C) in DNA from tumor tissue. Currently, there is a wealth of data showing that oxidative stress effecting through reactive oxygen species (ROS) plays a critical role in the etiology and progression of a number of human diseases. Oxidative damage to DNA, lipids, and proteins is deleterious for the cell. 5-methylcytosine, along with other DNA constituents, is a target for ROS, which results in the appearance of a variety of modified nucleic acid bases. Therefore, m5C moiety constitutes the mutational hotspot; it occurs either within a structural gene or its regulatory regions. Here, we show the results of the analysis of DNA extracted from brain tumor and other tissues. DNA was isolated and sheared by enzymatic digestion into nucleotides which, after labeling with [γ-32p]ATP, were separated on cellulose TLC. A chromatogram was evaluated using phosphoimager and an amount of m5dC was calculated as a ratio (R) of m5dC to m5dC+dC+dT spots intensities. The R values decrease as the malignancy of brain tumors increase. It is the important factor for differentiating low and high grade gliomas. It seems that DNA methylation pattern might be a useful tool for the diagnosis of brain tumors or as a marker for the early detection of the relapse of the disease.
  08/2009: pages 141-155;
• Article: Highly active low magnesium hammerhead ribozyme.

  Agnieszka Fedoruk-Wyszomirska, Maciej Szymański, Eliza Wyszko, Mirosława Z Barciszewska, Jan Barciszewski
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  ABSTRACT: Hammerhead (HH) ribozymes can be used for highly specific inhibition of gene expression through the degradation of target mRNA. In vitro experiments with minimal HH domains demonstrated that the efficiency of catalysis is highly dependent on concentration of magnesium ions. Optimal ion requirements for HH-catalysed RNA cleavage are far from these found in the cell. Recently, it has been proposed that the efficiency of HH ribozymes can be increased at low magnesium concentration through stabilization of a catalytically active conformation by tertiary interactions between helices I and II. We designed a ribozyme stabilized by GAAA tetraloop and its receptor motifs and demonstrated that it can efficiently catalyse target RNA hydrolysis at submillimolar Mg(2+) concentrations in vitro as well as in cultured cells. Both unmodified and locked nucleic acid-modified extended ribozymes proved superior to the minimal core ribozyme and DNAzyme against the same target sequence.
  Journal of biochemistry 02/2009; 145(4):451-9. · 1.95 Impact Factor
• Article: Isoenergetic microarray mapping reveals differences in structure between tRNAiMet and tRNAmMet from Lupinus luteus.

  Elzbieta Kierzek, Miroslawa Z Barciszewska, Jan Barciszewski
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  ABSTRACT: Isoenergetic microarray mapping is shown to be an ideal method for probing subtle structural differences between initiator tRNA(i)(Met) and elongator tRNA(m)(Met) from Lupinus luteus. The differences in structure of both tRNAs cause significant dissimilarities in binding to microarrays probes.
  Nucleic Acids Symposium Series 02/2008;
• Article: Analysis of 5-methylcytosine in DNA of breast and colon cancer tissues.

  Anna-Maria Barciszewska, Dawid Murawa, Iwona Gawronska, Pawel Murawa, Stanislaw Nowak, Miroslawa Z Barciszewska
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  ABSTRACT: 5-methylcytosine (m(5)C) can be used as a sensitive marker of progress of the tumor formation induced by the oxidative damage reactions. We have analyzed the amount of m(5)C in DNA of patients with breast and colon cancers. Two dimensional thin layer chromatography (TLC) has been used to monitor 5-methylcytosine level in DNA extracted from cancer tissues. The level of methylation of cytosine at C-5 position in DNA from breast cancer patients correlates well with the malignancy of tumors. Interestingly higher amount of m(5)C in DNA for the breast cancer patients treated with different chemotherapeutics was observed. It suggests an activation of DNA methyltransferase as well as a genomic suppression of the DNA repair genes expression. These differences clearly reflect the health condition of patients and support the global analysis of m(5)C in DNA as a good marker for diagnosis of neoplasia in clinical practice.
  International Union of Biochemistry and Molecular Biology Life 01/2008; 59(12):765-70. · 3.51 Impact Factor
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  Available from: psychepharmaceuticals.com

  Article: Suppression of human brain tumor with interference RNA specific for tenascin-C.

  Ryszard Zukiel, Stanislaw Nowak, Eliza Wyszko, Katarzyna Rolle, Iwona Gawronska, Miroslawa Z Barciszewska, Jan Barciszewski
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  ABSTRACT: Glioblastoma multiforme (GBM) accounts for approximately 12-15% of intracranial neoplasms. The GBM remains refractory to therapy because of tumor heterogeneity, local invasion, and non-uniform vascular permeability to drugs. Patients with GBM have the median survival of approximately 8-10 months, and for those cases where tumor recurs, the average time of tumor progression after therapy is only eight weeks. A combination of different treatment modes as surgery and chemo- or/and radiotherapy extend survival only for a short time, if any. Recently, tenascin-C (TN-C) as a dominant epitope in glioblastoma has been discovered. It is transiently expressed during organogenesis, absent or much reduced in most fully developed organs, but reappears under pathological conditions such as infection, inflammation, or tumorigenesis. It was found that the intensity of TN-C staining correlates with the tumor grade and that the strongest staining indicates poor prognosis.
  Cancer biology & therapy 09/2006; 5(8):1002-7. · 2.64 Impact Factor
• Article: Evaluation of the dynamic structure of DsrA RNA from E. coli and its functional consequences.

  Katarzyna Rolle, Marek Zywicki, Eliza Wyszko, Miroslawa Z Barciszewska, Jan Barciszewski
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  ABSTRACT: DsrA RNA is an 87-nucleotide regulatory non-protein-coding RNA of Escherichia coli for which two secondary structure models (I and II) have been proposed. We have compared these models by the energy calculations, which revealed that the currently accepted model II should be rejected on the basis of thermodynamics. Here we provide new results of nuclease footprinting analysis and the application of RNA technologies that have not previously been used for DsrA RNA structural studies, such as hydrolysis with RNase H, DNAzyme, hydroxyl radicals and lead. These approaches together with bioinformatics calculations provided strong arguments for a new model III. This model clearly shows that the long U-rich region between hairpins 1 and 2 is double-stranded. These findings shed new light on DsrA RNA-Hfq interactions.
  Journal of Biochemistry 04/2006; 139(3):431-8. · 2.37 Impact Factor
• Article: A new frontier for molecular medicine: noncoding RNAs.

  Maciej Szymanski, Miroslawa Z Barciszewska, Volker A Erdmann, Jan Barciszewski
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  ABSTRACT: It is now becoming evident that the variety of noncoding RNA (ncRNA) molecules play important roles in many cellular processes and they are not just mere intermediates in transfer of genetic information from DNA to proteins. Recent data, from the analyses of transcriptional activity of human genome, suggest that it may contain roughly equal numbers of protein- and RNA-encoding transcription units. Many of the ncRNAs described in humans as well as in other mammals have been linked, through specific chromosomal localization or expression patterns, with certain diseases including complex congenital syndromes, neurobehavioral and developmental disorders and cancer. These findings clearly indicate that an expression of genes of which end-products are RNA molecules is crucial for development, differentiation and normal functioning of the cells. The ncRNAs expression patterns can therefore be used as molecular markers for specific diagnostic methods.
  Biochimica et Biophysica Acta 10/2005; 1756(1):65-75. · 4.66 Impact Factor
• Article: Lead toxicity through the leadzyme.

  Miroslawa Z Barciszewska, Maciej Szymanski, Eliza Wyszko, Jakub Pas, Leszek Rychlewski, Jan Barciszewski
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  ABSTRACT: Lead is one of the most dangerous toxic agents for all living organisms. In humans, elevated levels of lead have been linked to a number of disorders for which various molecular mechanisms have been proposed. However, none of them has been fully understood. It has also been known for several years that at micromolar concentrations lead can bind a unique RNA motif and catalyze a site-specific hydrolysis of the polyribonucleotide chain. This motif, called leadzyme, may be one of the major targets for lead within the cell, and it can cleave various cellular RNAs. A search of GenBank revealed the sequences that can potentially fold into the structure containing the leadzyme motif and that they are rather common in eukaryotic genomes. We found that the domain occurs with a high frequency in human mRNA sequences. Thus, the leadzyme nucleolytic properties should be considered as a possible mechanism for destruction of RNA within a cell. In particular, targeting of the RNA scaffold of ribosomes or spliceosomes may explain lead-mediated toxicity leading to cell death.
  Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 04/2005; 589(2):103-10. · 2.85 Impact Factor
• Article: A simple epigenetic method for the diagnosis and classification of brain tumors.

  Ryszard Zukiel, Stanislaw Nowak, Anna-Maria Barciszewska, Iwona Gawronska, Gerard Keith, Miroslawa Z Barciszewska
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  ABSTRACT: The new, simple, and reliable method for the diagnosis of brain tumors is described. It is based on a TLC quantitative determination of 5-methylcytosine (m(5)C) in relation to its damage products of DNA from tumor tissue. Currently, there is evidence that oxidative stress through reactive oxygen species (ROS) plays an important role in the etiology and progression of several human diseases. Oxidative damage of DNA, lipids, and proteins is deleterious for the cell. m(5)C, along with other basic components of DNA, is the target for ROS, which results in the appearance of new modified nucleic acid bases. If so, m(5)C residue constitutes a mutational hotspot position, whether it occurs within a nucleotide sequence of a structural gene or a regulatory region. Here, we show the results of the analysis of 82 DNA samples taken from brain tumor tissues. DNA was isolated and hydrolyzed into nucleotides, which, after labeling with [gamma-(32)P]ATP, were separated on TLC. Chromatograms were evaluated using PhosphorImager and the amounts of 5-methyldeoxycytosine (m(5)dC) were calculated as a ratio (R) of m(5)dC to m(5)dC + deoxycytosine + deoxythymidine spot intensities. The R value could not only be a good diagnostic marker for brain tumors but also a factor differentiating low-grade and high-grade gliomas. Therefore, DNA methylation pattern might be a useful tool to give a primary diagnosis of a brain tumor or as a marker for the early detection of the relapse of the disease. This method has several advantages over those existing nowadays.
  Molecular Cancer Research 04/2004; 2(3):196-202. · 4.29 Impact Factor
• Article: A critical role of water in the specific cleavage of the anticodon loop of some eukaryotic methionine initiator tRNAs.

  Marcus Perbandt, Miroslawa Z Barciszewska, Christian Betzel, Volker A Erdmann, Jan Barciszewski
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  ABSTRACT: We have noticed that during a long storage and handling, the plant methionine initiator tRNA is spontaneously hydrolyzed within the anticodon loop at the C34-A35 phosphodiester bond. A literature search indicated that there is also the case for human initiator tRNA(Met) but not for yeast tRNA(i)Met or E. coli tRNA(f)Met. All these tRNAs have an identical nucleotide sequence of the anticodon stems and loops with only one difference at position 33 within the loop. It means that cytosine 33 (C33) makes the anticodon loop of plant and human tRNA(i)Met susceptible to the specific cleavage reaction. Using crystallographic data of tRNA(f)Met of E. coli with U33, we modeled the anticodon loop of this tRNA with C33. We found that C33 within the anticodon loop creates a pocket that can accomodate a hydrogen bonded water molecule that acts as a general base and catalyzes a hydrolysis of C-A bond. We conclude that a single nucleotide change in the primary structure of tRNA(i)Met made changes in hydration pattern and readjustment in hydrogen bonding which lead to a cleavage of the phosphodiester bond.
  Molecular Biology Reports 04/2003; 30(1):27-31. · 2.93 Impact Factor
• Article: 5S rRNA is a leadzyme. A molecular basis for lead toxicity.

  Miroslawa Z Barciszewska, Eliza Wyszko, Rolf Bald, Volker A Erdmann, Jan Barciszewski
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  ABSTRACT: This paper reports that the D-loop sequence of cellular mammalian ribosomal 5S RNAs is a natural leadzyme that specifically binds and cleaves in trans other RNA molecules in the presence of lead. The D-loops of these 5S rRNAs are similar in sequence to the active site of the leadzyme derived from tRNA(Phe), which cleaves a single bond in cis. We have devised a 12 nt model substrate based on the leadzyme sequence cleaved in trans by a 12 nt RNA molecule containing of the D-loop sequence. The model reaction occurs only at the appropriate concentration of lead and enzyme/substrate stoichiometry. The native 5S rRNA carries the same cleavage activity, although with different optimal lead concentration and stoichiometry. On the other hand, the isolated D-loop does not serve as a substrate when incubated with an RNA molecule with the potential to base pair with it and form the same internal loop (the bubble) present in the leadzyme-substrate complex. We show that the leadzyme cuts C-G, but not G-G or U-G linkages. The 5S rRNA leadzyme appears to have the shortest asymmetric pentanucleotide purine-rich loop flanked by two short double stranded RNAs. The leadzyme activity of native 5S rRNA may be an important aspect of lead toxicity in living cells. Because the leadzyme motif has been found in natural RNA species, its activity can be expressed in vivo even at a very low lead concentrations, of lead leading to the inactivation of other cellular RNAs. This might be one of the ways in which lead poisoning manifests itself at the molecular level. Lead toxicity is based not only on its binding to calcium and zinc binding proteins (such as Zn-fingers) and random hydrolysis of nucleic acids, but also, and most importantly, on the induction of the hydrolytic properties of RNA (RNA catalysis).
  Journal of Biochemistry 04/2003; 133(3):309-15. · 2.37 Impact Factor
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  Available from: 144.206.159.178

  Article: Native transfer RNA catalyzes Diels-Alder reaction.

  Michal Mielcarek, Miroslawa Z Barciszewska, Piotr Sałanski, Maciej Stobiecki, Janusz Jurczak, Jan Barciszewski
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  ABSTRACT: In this paper we show that transfer ribonucleic acids (tRNAs) catalyze the Diels-Alder cycloaddition reaction. A new DNA oxidative damage product, 6-furfuryladenine (kinetin) or its riboside (diene), was transformed with dimethyl acetylenedicarboxylate or maleic anhydride (dienophile). The reaction proceeds in the presence of tRNA at high pressure but not at ambient condition. If so tRNA in prebiotic conditions (RNA world) had at least two functions: catalytic and a carrier of genetic information. It means that tRNA at high pressure shows catalytic properties and is a true Diels-Alderase.
  Biochemical and Biophysical Research Communications 06/2002; 294(1):145-8. · 2.48 Impact Factor
• Article: 5S Ribosomal RNA Database.

  Maciej Szymanski, Miroslawa Z Barciszewska, Volker A Erdmann, Jan Barciszewski
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  ABSTRACT: Ribosomal 5S RNA (5S rRNA) is an integral component of the large ribosomal subunit in all known organisms with the exception only of mitochondrial ribosomes of fungi and animals. It is thought to enhance protein synthesis by stabilization of a ribosome structure. This paper presents the updated database of 5S rRNA and their genes (5S rDNA). Its short characteristics are presented in the Introduction. The database contains 2280 primary structures of 5S rRNA and 5S rRNA genes. These include 536 eubacterial, 61 archaebacterial, 1611 eukaryotic and 72 organelle sequences. The database is available on line through the World Wide Web at http://biobases.ibch.poznan.pl/5SData/.
  Nucleic Acids Research 02/2002; 30(1):176-8. · 8.03 Impact Factor
• Article: Some unusual nucleic acid bases are products of hydroxyl radical oxidation of DNA and RNA

  Jan Barciszewski, Miroslawa Z. Barciszewska, Gunhild Siboska, Suresh I.S. Rattan, Brian F.C. Clark
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  ABSTRACT: There are over 100 modified bases and their derivatives found in RNA and DNA. For some of them, data concerning their properties, synthesis and roles in cellular metabolism are available, but for others the knowledge of their functions and biosynthetic pathways is rather limited. We have analysed the chemical structure of modified nucleosides of DNA and RNA considering mainly their putative synthetic routes. On this basis we suggest, that in addition to enzymatic biosynthetic pathways well established for some odd bases, many rare nucleosides can be recognised as products of random chemical reactions. We identify them as primary or secondary products of the reaction of nucleic acids with hydroxyl radicals, the most active oxidising agent in the cell.
  Molecular Biology Reports 11/1999; 26(4):231-238. · 2.93 Impact Factor
• Article: S rRNA Data Bank

  Maciej Szymanski, Thomas Specht, Miroslawa Z Barciszewska, Jan Barciszewski, Volker A Erdmann
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  ABSTRACT: In this paper we present the updated version of the compilation of 5S rRNA and 5S rDNA nucleotide sequences. It contains 1622 primary structures of 5S rRNAs and 5S rRNA genes from 888 species. These include 58 archaeal, 427 eubacterial, 34 plastid, nine mitochondrial and 1094 eukaryotic DNA or RNA nucleotide sequences. The sequence entries are divided according to the taxonomic position of the organisms. All individual sequences deposited in the 5S rRNA Database can be retrieved using the WWWbased, taxonomic browser at http://rose.man.poznan. pl/5SData/5SRNA.html or http://www.chemie.fu-berlin. de/fb_chemie/agerdmann/5S_rRNA.html . The files with complete sets of data as well as sequence alignments are available via anonymous ftp. INTRODUCTION This data bank was prepared in August and September 1997. It is just 30 years since the first nucleotide sequence of 5S rRNA was determined (1) and 35 years since this RNA species was been identified for the first time as a component of the larg...
  01/1998;
• Article: The primary structure ofHarpalus rufipes 5S ribosomal RNA

  Miroslawa Z. Barciszewska, Arkadiusz Gawroński, Maciej Szymański, Volker A. Erdmann
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  ABSTRACT: The nucleotide sequence of 5S ribosomal RNA from the beetleHarpalus rufipes was determined and compared with primary structures of other insect 5S rRNAs. Sequence differences between two beetle 5S rRNAs may represent phylogenetic markers specific for two groups of Coleoptera — Adephaga and Polyphaga. Analysis of all insect sequences using parsimony allowed us to infer a phylogenetic tree of insects, which is consistent with morphological and paleobiological data.
  Molecular Biology Reports 09/1995; 21(3):165-167. · 2.93 Impact Factor
• Article: A new model for the tertiary structure of 5S ribonucleic acid in plants

  Miroslawa Z. Barciszewska, Volker A. Erdmann, Jan Barciszewski
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  ABSTRACT: Chemical, enzymatic and physicochemical methods of a structural analysis of 5S rRNAs in lupine, wheat germ, and other plants led us to propose a new three-dimensional model of these molecules The main features of the model are tertiary interactions between the β- and γ-domains of the molecule, specifically nucleotides (34)CCCA(37) in loop C and nucleotides (85)GGGU(88) in loop D. In addition we propose tertiary base-pairing in A100-U53 between loops B and E. We have confirmed this model by NMR spectroscopy and by chemical modification with diethylpyrocarbonate. Our results are consistent with the proposed model and are also applicable to all eukaryotic 5S rRNAs. Our model is clearly differentiated from others by intramolecular tertiary hydrogen bonds between the two domains.
  Plant Molecular Biology Reporter 01/1994; 12(2):116-131. · 2.45 Impact Factor