Junhai Huang

East China University of Science and Technology, Shanghai, Shanghai Shi, China

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Publications (6)26.29 Total impact

  • Junhai Huang, Yufang Xu, Xuhong Qian
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    ABSTRACT: 8-Aminoquinoline (8-AQ) and 2-aminopyridine (AmPyr) both as ionophoric agents were robustly used in development of a cation sensor. To evaluate the effect of complexation with cations, flexible 2-aminopyridine and rigid 8-AQ moiety were directly introduced into rhodamine chromophore and two candidate fluorescent sensors were constructed successfully. with a rigid 8-AQ fragment exhibited turn-on fluorescence and color responses to Fe(3+) ion over other metal ions related to biology in aqueous solution. The sensor with a flexible 2-aminopyridine did not give positive responses to cations although with a similar binding cavity as on . Furthermore, the 2 : 1 recognition mode of with Fe(3+) was proved according to the 1D and 2D COSY H-H NMR experiments. The live cell imaging experiments demonstrated that could be successfully applied as a bioimaging agent for monitoring Fe(3+) in living cells.
    Dalton Transactions 01/2014; · 3.81 Impact Factor
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    ABSTRACT: A new probe based on the Gabriel mechanism was designed and first used for hydrazine detection with high selectivity against other amines in aqueous solution. Importantly, the probe could be used for gas-state discrimination of hydrazine with different concentrations. Additionally, probe could also be applied for the imaging of hydrazine in living cells.
    Chemical Communications 12/2013; · 6.38 Impact Factor
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    Junhai Huang, Yufang Xu, Xuhong Qian
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    ABSTRACT: 4,5-Diamino-1,8-naphthalimide (DNP)-based chemosensor was designed and synthesized. Probe specifically recognized Cu(2+) ions in neutral aqueous solution. The capture of Cu(2+) by the receptor resulted in deprotonation of the secondary amine conjugated to the 1,8-naphthalimide chromophore, so that the electron-donation ability of the "N" atom would be greatly enhanced; thus probe showed a 50 nm red-shift in absorption (from 464 nm to 514 nm) and a large colorimetric response, it also exhibited an on-off fluorescent response. Specifically, is a pH-independent sensor in the range of 6.0 to 12.0 and can be used as a probe for Cu(2+) in strong basic conditions. These results opened up new possibilities for construction of red-shift colorimetric and fluorescent sensors.
    Organic & Biomolecular Chemistry 05/2009; 7(7):1299-303. · 3.57 Impact Factor
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    Junhai Huang, Yufang Xu, Xuhong Qian
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    ABSTRACT: A Cu2+-specific colorimetric sensor of a 1,8-naphthalimide chromophore integrated with a 2-aminodiphenylamine (2-adpa)-based receptor was designed and synthesized. Sensor had high selectivity and sensitivity for Cu2+ in neutral aqueous buffer solution. The analytical detection limit (ADL) was 3.0 x 10(-7) M. Based on the Cu2+-induced deprotonation of the diarylamino "NH" moiety, the coordination mode was proposed with 1:1 stoichiometry between and Cu2+. These results opened up possibilities for the construction of novel colorimetric sensors with a red-shift absorption in a D-pi-A system.
    Dalton Transactions 04/2009; · 3.81 Impact Factor
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    Junhai Huang, Yufang Xu, Xuhong Qian
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    ABSTRACT: A rhodamine-based sensor 1 was designed and synthesized by incorporation the rhodamine fluorophore and ionophore NS(2) with high affinity to Hg(2+). Sensor 1 exhibits a high selectivity and an excellent sensitivity and is a dual-responsive colorimetric and fluorescent Hg(2+)-specific sensor in aqueous buffer solution. In addition, the 1:1 binding mode was proposed based on the (1)H NMR and ES(+)MS studies.
    The Journal of Organic Chemistry 03/2009; 74(5):2167-70. · 4.56 Impact Factor
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    ABSTRACT: Recently novel synthetic jasmonate derivatives were shown to have very powerful stimulating effects on the biosynthesis of taxuyunnanine C (Tc) by Taxus chinensis cells (Biotech Bioeng, 86:595; ibid. 86: 809, 2004). To provide an insight into the elicitation mechanism of the newly synthesized elicitors, by taking 2-hydroxyethyl jasmonate (HEJ, at 100 microM) as a typical example, in this work the defense signals were detected and their influences on the expression of important genes in taxoid biosynthetic pathway were examined in cell cultures of T. chinensis. The oxidative burst (induced H2O2 production) was confirmed, and the induction of lipoxygenase (LOX) activity and intracellular jasmonate acid (JA) synthesis was found. The gene transcription of geranylgeranyl diphosphate synthase (GGPPs) and taxa-4(5),11(12)-diene synthase (TS) was up-regulated by HEJ elicitation compared to control. Inhibition of JA biosynthesis by a putative LOX inhibitor, ibuprofen (IBU), effectively depressed the HEJ-induced up-regulation of GGPPs and TS gene transcription levels. In contrast, the inhibition of H2O2 production by membrane NADPH oxidase inhibitor, diphenylene iodonium (DPI), did not affect the transcriptions of those genes. For the HEJ-induced Tc production, it was suppressed with addition of DPI or IBU. The results suggest that both H2O2 and JA signals were involved in HEJ-induced Tc biosynthesis, and JA mediated the induction of GGPPs and TS genes expression, but H2O2 was not essential to activate them. Finally, a signal transduction cascade from defense signal response to activated transcription of taxoid biosynthetic genes and enhanced Tc production is proposed.
    Biotechnology and Bioengineering 09/2006; 94(6):1064-71. · 4.16 Impact Factor