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ABSTRACT: To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric cancer.
A peptide screen was performed by biopanning the PhD-12 phage display library, clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer tissues. Tumor-targeted binding of selected peptides was confirmed by bound phage counts, enzyme-linked immunosorbent assay, competitive inhibition, fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer tissues.
Approximately 92.8% of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normal- appearing gastric mucosa. After the third round of positive screening, the peptide sequence AADNAKTKSFPV (AAD) appeared in 25% (12/48) of the analyzed phages. For the control peptide, these values were 6.8 ± 2.3, 5.1 ± 1.7, 3.5 ± 2.1, 4.6 ± 1.9 and 1.1 ± 0.5, respectively. The values for AAD peptide were statistically significant (P < 0.01) for gastric cancer as compared with other histological classifications and control peptide.
A novel peptide is discovered to have a specific binding activity to gastric cancer, and can be used to distinguish neoplastic from normal gastric mucosa, demonstrating the potential for early cancer detection on endoscopy.
World Journal of Gastroenterology 05/2012; 18(17):2053-60. · 2.47 Impact Factor
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ABSTRACT: Cancer is a disease that does great harms to the health of human beings. FT-IR spectroscopy could identify variability at the molecular level in biological specimens. It is a rapid and noninvasive method, which could be used intraoperatively to modify surgical procedures. The aim of this paper is to identify and separate cancer from colitis in endoscopic colon biopsies through the use of FT-IR spectroscopy. A total of 88 endoscopic colon samples, including 41 cases of colitis and 47 cases of colon cancer, were obtained. Specimens were placed on an ATR accessory linked to FT-IR spectrometer with a MCT detector for greater stability and sensitivity. Later, specimens were sent for the histological examination as the reference in the spectral analysis. 41 colitis and 47 cancer specimens were compared. Spectra preprocessed with smoothing and normalization were used for discrimination analysis. PCA was processed to simplify the spectrum data set. Naive Bayes classifier model was constructed for diagnostic classification. Leave-one-out cross-validation method was utilized to assess the discrimination results. The sensitivity of FT-IR detection for cancer achieves 97.6%. The results showed that colon cancer could be distinguished from colitis with high accuracy using FT-IR spectroscopy and chemometrics.
TheScientificWorldJOURNAL 01/2012; 2012:936149. · 1.66 Impact Factor
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ABSTRACT: The homeobox gene, CDX2, plays a major role in development, especially in the gut, and also functions as a tumor suppressor in the adult colon. In the present study, we investigated the effects of CDX2 expression on the proliferation, migration, and apoptosis of the human colon cancer cell line, Lovo. Lovo cells exogenously expressing CDX2 exhibited no significant differences in the percentage of cells in G1- and S-phase or in apoptosis, as determined by flow cytometry. MTT assay also confirmed that CDX2 expression had no effect on proliferation in these cells. Interestingly, conditioned medium collected from CDX2-overexpressing Lovo cells showed a significant decrease in secretion of MMP-2 and the invasive potential of these cells was significantly inhibited. Collectively, these data suggest that CDX2 may play a critical role in the migration and metastasis of colon carcinoma and over-expression of CDX2 in colon cancer cells markedly inhibits invasion. Based on these results, exogenous expression of CDX2 might be a promising option in the treatment of colon carcinoma.
Pathology & Oncology Research 04/2011; 17(3):743-51. · 1.37 Impact Factor
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ABSTRACT: To evaluate the inhibitory effect of lymphocytes activated by the superantigen of toxic shock syndrome toxin-1 (TSST-1), which is regulated synergistically by 5 copies of hypoxia-responsive element (5HRE) and promoter of carcino-embryonic antigen (CEAp), against the carcino-embryonic antigen (CEA)-positive human colon carcinoma cell line LoVo under hypoxia condition in vitro.
The eukaryotic expressive vector was constructed with the transmembrane superantigen gene of 5HRE-CEAp-regulated TSST-1-linker-CD80TM, and was transfected into CEA-positive human colon carcinoma cell line LoVo and CEA-negative human cervical carcinoma cell line HeLa by Lipofectamine 2000. Stably transfected cell lines were selected by G418. RT-PCR was employed to examine the expression of TC mRNA. Peripheral blood lymphocytes(PBL) of healthy people were extracted and then activated by the lysates of tumor cells stably transfected with TC. Concurrently, PBL were cultured together with stably transfected tumor cells in order to kill these cells. Then the proliferative effect of TSST-1 on PBL, and the killing effect of PBL against the stably transfected tumor cells were detected by MTT.
Monoclonal LoVo and HeLa cells stably transfected with TC were successfully obtained. Expression of TC mRNA in monoclonal LoVo cells under hypoxia condition was significantly higher than those under normoxia condition as confirmed by RT-PCR. Monoclonal HeLa cells did not express TC under either hypoxia condition or normoxia condition. As is shown by MTT assay, TSST-1 expressed by the monoclonal LoVo cells could effectively activate PBL to proliferate under hypoxia condition, resulting in dose-dependent inhibition on the proliferation of LoVo cells that expressed TSST (P<0.05). But HeLa cells and wild LoVo cells could not activate PBL to proliferate. PBL could not inhibit proliferation of HeLa cells and wild LoVo cells, either.
The superantigen of TSST-1 regulated dually by 5HRE and CEAp could activate human PBL to kill CEA-positive tumor cells specifically under hypoxia condition.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 06/2010; 26(6):525-9.
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ABSTRACT: The aim of the present research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared spectra) of the cultured carcinoma cell and nucleus with FTIR spectroscopy, and investigating the special spectral features of the carcinoma cell and nucleus compared with the carcinoma tissues. In this paper, the gallbladder carcinoma tissues confirmed by histology were measured using a Nicolet Magna 5700-II FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gallbladder carcinoma cell (GBC-SD) and nucleus were centrifuged to provide a small pellet of cell and nucleus for FTIR analysis. The cell and nucleus pellet was then placed on the OMNIC sampler. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cell and nucleus (GBC-SD) and that of the corresponding gallbladder tissues. Several infrared spectral features of the carcinoma cell and nucleus were obtained. All the results suggest that the spectral features of the carcinoma cell and nucleus can be well reflected by that of the carcinoma tissue, though the later is more complicated, which might originate from the intrinsic complexity of the tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
Guang pu xue yu guang pu fen xi = Guang pu 07/2009; 29(7):1750-3. · 0.84 Impact Factor
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ABSTRACT: In the present paper, probabilistic neural network method was applied to the classification of gastric endoscope samples based on FTIR spectroscopy for higher discrimination correctness than the conventional linear discriminant analysis algorithm. The probabilistic neural network (PNN) is a kind of radial basis network suitable for discriminant analysis. There are several advantages of PNN method: less time is needed to train the model, higher correctness could be achieved, global optimal solution could be obtained and so on. In this paper, PNN method was utilized to classify gastric endoscopic biopsies into healthy, gastritis, and malignancy. Firstly, principal component analysis was carried out for the pretreated sample spectra. Principal components analysis is a quantitatively rigorous method for achieving the simplification. The method generates a new set of variables, called principal components. Each principal component is a linear combination of the original variables. All the principal components are orthogonal to each other, so there is no redundant information. The principal components as a whole form an orthogonal basis for the space of the data. And then, the scores of principal components were selected as input to train the PNN model. Finally, PNN model was established. In this experiment, a total of 118 gastric endoscopic biopsies, including 35 cases of cancer, 64 cases of gastritis, and 19 healthy tissue samples, were obtained at the First Hospital of Xi'an Jiaotong University, China. Fifty nine samples were selected to establish the PNN classification model. The rest of the samples were used as the test set to valid the discriminant analysis model. The total discrimination correctness of normal, inflammation and gastric cancer achieved 81.4%.
Guang pu xue yu guang pu fen xi = Guang pu 06/2009; 29(6):1553-7. · 0.84 Impact Factor
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ABSTRACT: Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line.
The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF2 window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue.
Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue.
The diagnosis of carcinoma tissue by FT-IR spectroscopy has a sufficient cellular basis.
Hepatobiliary & pancreatic diseases international: HBPD INT 03/2009; 8(1):75-8. · 1.08 Impact Factor
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ABSTRACT: The aim of this research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared) spectra of the cultured carcinoma cells with FTIR spectroscopy and investigating the special spectral features of the carcinoma cells compared with the carcinoma tissues. In the present paper, the gastric carcinoma tissues confirmed by histology were measured using a Nicolet Magna750-II FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gastric carcinoma cells (SGC7901) were centrifuged to provide a small pellet of cells for FTIR analysis. The cell pellet was then placed on a specially designed salt plate made of BaF2. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cells (SGC7901) and that of the corresponding gastric tissues. Several infrared spectral features of the carcinoma cells were obtained: the different bands between cells and tissues locate in the range of 3 000-3 600 cm(-1) and 1 640 cm(-1) which are the range of the hydroxy stretching and blending bands of H2O. There are more H2O out of carcinoma cells in carcinoma tissues, so the strong bands of H2O cover the distinctive bands of carcinoma cells in carcinoma tissues. Although the carcinoma tissue is more complicated, which might originate from the intrinsic complexity of the tissue, the results suggest that the spectral features of the carcinoma cells can be well reflected by that of the carcinoma tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
Guang pu xue yu guang pu fen xi = Guang pu 02/2008; 28(1):51-4. · 0.84 Impact Factor
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ABSTRACT: The patient with malignant tumor always show immunologic function drawback and ingravescent with tumor development, especially in the aspect of cell-mediated immunity. This study was undertaken to define the relationship between the immune function of local cells and cancer development by investigating the distribution of natural killer (NK) cells and T-lymphocyte subsets in peripheral blood, the cancer tissue and the tissue surrounding gallbladder carcinoma.
The numbers of CD4+ and CD8+ T-lymphocytes and NK cells were measured by flow cytometry in samples taken from gallbladder cancer tissue, the surrounding tissues and peripheral blood of 38 patients, and compared with the numbers in the peripheral blood and gallbladder tissue of 30 patients with cholecystitis as controls.
The numbers of CD4+ and CD8+ T-cells and NK cells in gallbladder cancer tissues were significantly higher than those in the surrounding tissue and gallbladder with gallstone. However, the ratio of CD4+/CD8+ was lower in the cancer tissue than that in the surrounding tissue and tissue from gallbladders with gallstones. The distribution of CD4+ and CD8+ T-cells and NK cells in mucous membrane of cholecystitis gallbladder and that in the tissue surrounding gallbladder cancer were significantly different.
Disproportionate and imbalanced distribution of NK cells and subsets of T-lymphocytes occurs in the mucous membrane proper of gallbladder cancer and surrounding tissue. Although gallbladder cancer tissue has higher expressions of CD4+, CD8+ and NK cells, the immune function is low or in an inhibited state. In gallbladder cancer immunization therapy, local cellular immunological function should be enhanced and the protective barrier improved.
Hepatobiliary & pancreatic diseases international: HBPD INT 03/2007; 6(1):81-6. · 1.08 Impact Factor
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ABSTRACT: To determine if Fourier-transform infrared (FT-IR) spectroscopy of endoscopic biopsies could accurately diagnose gastritis and malignancy.
A total of 123 gastroscopic samples, including 11 cases of cancerous tissues, 63 cases of chronic atrophic gastritis tissues, 47 cases of chronic superficial gastritis tissues and 2 cases of normal tissues, were obtained from the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance (ATR) accessory was linked to a WQD-500 FT-IR spectrometer for spectral measurement followed by submission of the samples for pathologic analysis. The spectral characteristics for different types of gastroscopic tissues were summarized and correlated with the corresponding pathologic results.
Distinct differences were observed in the FT-IR spectra of normal, atrophic gastritis, superficial gastritis and malignant gastric tissues. The sensitivity of FT-IR for detection of gastric cancer, chronic atrophic gastritis and superficial gastritis was 90.9%, 82.5%, 91.5%, and specificity was 97.3%, 91.7%, 89.5% respectively.
FT-IR spectroscopy can distinguish gastric inflammation from malignancy.
World Journal of Gastroenterology 08/2005; 11(25):3842-5. · 2.47 Impact Factor
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ABSTRACT: Fourier transform infrared (FT-IR) spectroscopy is an effective tool for investigation of chemical changes at the molecular level. We previously demonstrated that FT-IR spectroscopy can reliably distinguish multiple types of carcinoma from healthy tissue. Because various stomach diseases are common, it is important to explore a noninvasive and rapid method to detect malignancy and gastritis in endoscopic biopsies. Our aim was to classify endoscopic biopsies into healthy, gastritis, and malignancy through the use of FT-IR spectroscopy.
A total of 103 endoscopic samples, including 19 cases of cancer, 35 cases of chronic atrophic gastritis, 29 cases of chronic superficial gastritis, and 20 healthy tissue samples, were obtained at the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance accessory was linked to a WQD-500 FT-IR spectrometer for biopsy measurement. The spectral characteristics for different types of tissues were correlated with the corresponding pathology results. The gastric biopsies were classified by FT-IR spectroscopy and a discriminant analysis method.
There were significant differences in the FT-IR spectra of four types of gastric biopsies. The discriminant analysis results demonstrated that the sensitivity of FT-IR detection for healthy, superficial gastritis, atrophic gastritis, and gastric cancer was 90%, 90%, 66%, 74%, respectively, which could help satisfy clinical diagnostic requirements.
FT-IR spectroscopy can distinguish disease processes in gastric endoscopic biopsies.
Clinical Chemistry 03/2005; 51(2):346-50. · 7.91 Impact Factor
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ABSTRACT: With the wide use of B-ultrasonography in recent years, the polypoid lesion of the gallbladder (PLG) has been one of the most common diseases detected in biliary surgery. This study was to investigate the diagnostic method and operative indications of PLG.
The clinical and pathological data of 194 patients with PLG who had received operation at our hospital from January 1994 to September 2002 were analyzed retrospectively. Categorized data were analyzed by the chi-square test.
All the patients received preoperative B-ultrasonography. 185 of the 194 PLG patients were diagnosed as having cholecystic polyp, and 9 adenomas. Among the 42 patients who received CT, 6 showed early gallbladder cancer. Pathologically, cholesterol polyps were mostly multiple lesions (64.7%) with a mean diameter of 3.86+/-2.2 mm in 136 patients. Of 16 patients with adenomas, 10 had a tumor diameter of more than 10 mm (62.5%). In 11 patients with gallbladder carcinoma, 7 were accompanied with gallbladder stone (63.6%). In addition, inflammatory polyps and adenomyomas were found in 25 and 6 patients respectively.
B-ultrasonography is the most effective diagnostic method for detecting PLG. When large or irregular lesions are found, CT should be performed in order to avoid missing of gallbladder carcinoma. Operative indications for PLG include: a maximal tumor diameter of more than 10 mm; an over 50-year-old patient with a widebase and a single polyp lesion; a wide-base lesion or a lesion showing a tendency to enlargement; co-existing gallbladder stone or cholecystitis; a patient without other diseases but obvious clinical features and failure of general management; big or long pedicels or polyps at the neck of the gallbladder for preventing the empty of the gallbladder and a history of biliary colic; and PLG with irregularly thickened local gallbladder wall.
Hepatobiliary & pancreatic diseases international: HBPD INT 12/2004; 3(4):591-4. · 1.08 Impact Factor
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ABSTRACT: A total of 184 specimens from gastroscopy were studied using Fourier transform infrared (FTIR) spectroscopy. The spectra of small-sized gastric endoscope samples with high quality were measured using the modified FTIR spectrometer with a MCT detector. The results show that the spectra of chronic superficial gastritis are similar to those of the normal block tissue in our previous study. But there are significant differences in band location and relative intensity between the spectra of chronic superficial gastritis and those of normal gastric tissues. Similarly, the differences exist in the spectra of chronic atrophic gastritis and those of malignant gastric tissues. In addition, the subtraction technique can provide more information to identify well the gastric samples of chronic atrophic gastritis, superficial gastritis and gastric cancer from endoscope the detection. These results indicate that FTIR spectroscopy exhibits potential to diagnose the gastric samples from gastroscopy.
Guang pu xue yu guang pu fen xi = Guang pu 09/2004; 24(8):933-5. · 0.84 Impact Factor
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ABSTRACT: Some reports have confirmed that occurrence and development of tumor are related with lots of oncogene, anti-oncogene and cytokine. This study was to detect the expression of TNFmRNA, tumor necrosis factor (TNF) and TNF receptor (TNFR) in the gallbladder mucosa which developed from hyperplasia, dysplasia to carcinoma, and to further discuss the pathogenecity of gallbladder carcinoma.
The expression of TNFmRNA, TNF and TNFR was detected by in situ hybridization and immunohistochemistry on the surgical specimens of hyperplasia, dysplasia and carcinoma of the gallbladder.
The percentage of positive cells expressed TNFmRNA in the gallbladder mucosa was 0%, 20%, and 90%, respectively in hyperplasia, dysplasia and carcinoma (P<0.05). The percentage of positive mononuclear cells (MNCs) expressed TNFmRNA in hyperplasia, dysplasia and carcinoma of gallbladder tissues was 15%, 85%, and 90%, respectively (P<0.05). The number of positive MNC high-power field (Hpf) was 4.85+/-1.50, 6.00+/-2.71, and 9.33+/-3.07, respectively (P<0.05). The number of carcinoma cells and MNCs expressed by TNFmRNA per high-power field was increased with the increase of tumor stage. The number of carcinoma cells/Hpf in stages I-III and IV-V was 9.13+/-4.39 and 7.13+/-2.53 (P<0.05), and that of MNC/Hpf was 14.80+/-4.02 and 11.10+/-2.23 (P<0.01). The number of carcinoma cells and MNCs expressed by TNFmRNA per Hpf was increased with the increase of tumor size. In tumors of more than 2 cm or less than 2 cm in diameter, the number of positive carcinoma cells/Hpf was 14.00+/-4.20 and 8.83+/-4.96, respectively (P<0.05), but that of MNC/Hpf was 10.50+/-2.54 and 7.00+/-2.83 (P<0.05). The pattern of TNF protein expression was similar to that of TNFmRNA, whereas TNF protein expression was more frequent and extensive than TNFmRNA expression. TNFR was expressed in endothelial cells and MNCs of carcinoma, and was negative in mucosal epithelial cells and tumor cells. A positive linear correlation in TNFmRNA expression was observed between tumor cells and MNCs (r=0.687, P<0.01), a correlation in TNFmRNA and TNF protein expression of tumor cells (r=0.847, P<0.001), and a correlation in TNFmRNA and TNF protein expression of MNC in tumor tissue (r=0.643, P<0.01).
TNFmRNA and TNF protein expression is increased during the development of gallbladder mucosa from hyperplasia, dysplasia to carcinoma, and is increased with tumor stage. This finding suggests that TNF is involved in the pathogenesis of gallbladder carcinoma induced by gallstones and the TNF expression in cancer cells may serve as a marker for tumor stage.
Hepatobiliary & pancreatic diseases international: HBPD INT 08/2004; 3(3):448-52. · 1.08 Impact Factor
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ABSTRACT: To investigate the relationship between severe acute pancreatitis (SAP) and organ failure.
Clinical data of 74 cases of SAP from Jan. 1993 to Dec. 2002 were retrospectively reviewed, and the relationship between organ failure and age, gender, etiology, extent of necrosis, infection of necrosis and mortality was analyzed.
A total of 47 patients (63.5%) showed organ failure, 20 patients (27.0%) multiple organ failure, whereas 27 patients (36.5%) with dysfunction of a single organ system. Pulmonary failure was the most common organ dysfunction (23.0%) among single organ failures. There were no significant differences in age, gender and gallstone pancreatitis among patients with or without organ failure (P>0.05). The incidence of organ failure in infected necrosis was not higher compared with sterile necrosis, and patients with increased amount of necrosis did not have an increased prevalence of organ failure (P>0.05). Patients with organ failure had a higher mortality rate compared with those without organ failure (P<0.05). The death of SAP was associated with multiple organ failure (P<0.005), pulmonary failure (P<0.005), cardiovascular dysfunction (P<0.05) and gastrointestinal dysfunction (P<0.05).
Organ failure is common in patients with SAP, and patients with multiple organ failure and pulmonary failure have a higher mortality rate. Prevention and active treatment of organ failure can improve the outcome of patients with SAP.
World Journal of Gastroenterology 11/2003; 9(11):2570-3. · 2.47 Impact Factor
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ABSTRACT: To study the risk factors for death of patients with severe acute pancreatitis (SAP) within 24 hours after admission.
Clinical and laboratory data of 74 patients with SAP were analyzed retrospectively. The 27 possible risk factors for death within 24 hours after admission were investigated using logistic regression (SPSS software package 10.0), and the equation of logistic regression was set up.
Among the 27 possible risk factors, arterial pH, APACHE II scores, early shock, multiple organ failure were associated with mortality. Single logistic regression analysis of the 27 parameters showed that early shock, pleural effusion, arterial pH, complications and APACHE II scores were associated with death of SAP patients, but using multiple logistic regression analysis showed that only acidosis (pH<7.35) was associated with death.
To maintain the function of organs and correct dysequilibrium of water-electrolyte and acid-base in early treatment of SAP is essential to lower the mortality.
Hepatobiliary & pancreatic diseases international: HBPD INT 08/2003; 2(3):453-7. · 1.08 Impact Factor
