Yanping Zhang

Publications (10)14.06 Total impact

• Article: Estrogen inhibits vaginal tropoelastin and TGF-β1 production.

  Nahida Chakhtoura, Yanping Zhang, Keith Candiotti, Carlos A Medina, Peter Takacs
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  ABSTRACT: INTRODUCTION AND HYPOTHESIS: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. METHODS: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. RESULTS: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. CONCLUSION: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.
  International Urogynecology Journal 06/2012; · 1.83 Impact Factor
• Article: Effects of PPAR-delta agonist and zinc on vaginal smooth muscle cells collagen and tropoelastin production.

  Peter Takacs, Yanping Zhang, Keith Candiotti, Sindy Jaramillo, Carlos A Medina
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  ABSTRACT: INTRODUCTION AND HYPOTHESIS: The objective was to measure the effects of peroxisome proliferator-activated receptor delta (PPAR-delta) agonist and zinc sulfate (ZS) on vaginal smooth muscle cell (SMC) proliferation, tropoelastin production, and collagen production. METHODS: SMC cultures were performed from vaginal wall biopsies and were incubated with ZS and PPAR-delta agonist GW501516 (GW). Cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Supernatants and cell lysates were collected. Tropoelastin production was measured by Fastin Elastin Assay and collagen was measured with Sircol Collagen Assay. RESULTS: SMC proliferation was similar with 20 μM ZS or 10 nM GW compared to control. Tropoelastin production was significantly increased by 20 μM ZS and by 10 nM GW. Cell culture surface deposited elastin production was significantly increased by 20 μM ZS and by the combination of 20 μM ZS with 10 nM GW, and collagen production was significantly increased by 10 nM GW and by the combination of 20 μM ZS with 10 nM GW. CONCLUSIONS: PPAR-delta agonist and ZS increases vaginal SMC tropoelastin and collagen production.
  International Urogynecology Journal 05/2012; · 1.83 Impact Factor
• Article: CYP2D6 poor metabolizer genotype and smoking predict severe postoperative pain in female patients on arrival to the recovery room.

  Zongqi Yang, Zhe Yang, Kristopher L Arheart, Richard Morris, Yanping Zhang, Yiliam Rodriguez, Chunyu Song, Melvin C Gitlin, Keith A Candiotti
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  ABSTRACT: Recent studies have shown that CYP2D6 acts at critical steps for endogenous morphine biosynthesis. The present study assessed the contribution of CYP2D6 genetic polymorphisms, smoking, and other factors on acute severe postoperative pain (linear analog pain scores ≥8). Two hundred thirty-six female patients were found to have adequate information in a previously developed female surgical patient database to be included in this current analysis. Multiple logistic regression analysis was used to assess the predictors for acute severe postoperative pain. DNA had been previously extracted from blood samples in all patients and was genotyped by the Amplichip to determine the specific CYP2D6 genotypes. It was noted that the incidence of acute severe postoperative pain (linear analog pain scores ≥8) was more frequent in patients with the CYP2D6 poor metabolizer (PM) genotype, 71%, compared with 28% in intermediate metabolizers (IMs), 26% in extensive metabolizers (EMs), and 27% in ultrarapid metabolizers (UMs). The overall association between metabolizer groups and severe postoperative pain was significant (P=0.023). PMs were significantly more likely to suffer from severe postoperative pain than IMs, EMs, and UMs (P=0.007, 0.002, and 0.050, respectively). There were no significant differences among IMs, EMs, and UMs. Additionally, it was noted that there was an increased frequency of acute severe postoperative pain in smokers vs nonsmokers (P=0.014). This study demonstrated that female patients possessing the PM genotype of CYP2D6 and patients who smoke had a higher incidence of acute severe postoperative pain.
  Pain Medicine 04/2012; 13(4):604-9. · 2.35 Impact Factor
• Article: TGF-beta 1 is a potential regulator of vaginal tropoelastin production.

  Peter Takacs, Yanping Zhang, Sujata Yavagal, Keith Candiotti, Nahida Chakhtoura, Carlos A Medina
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  ABSTRACT: Our aims were to correlate transforming growth factor (TGF)-β1 and elastin mRNA expression in the vagina of women and to measure the effects of TGF-β1 on vaginal smooth muscle cell (SMC) proliferation and tropoelastin production. Vaginal walls were sampled in women (n = 20). TGF-β1 and elastin mRNA expression was assessed by RT-PCR. SMC cultures were performed from vaginal wall biopsies. SMC were incubated with TGF-β1, and cell proliferation was assessed by MTT-assay. Tropoelastin production was measured by the Fastin Elastin Assay. There was a significant positive correlation between TGF-β1 and elastin mRNA (r = 0.784, P < 0.01). SMC proliferation was significantly increased by 10 ng/mL TGF-β1 [relative cell number, mean ± SD, 198% ± 32% of control (P = 0.01)]. Tropoelastin production was significantly increased by TGF-β1 [mean ± SD, 645% ± 180% of control (P = 0.01)]. There is a positive correlation between TGF-β1 and elastin mRNA expression in the vaginal wall. In vitro, TGF-β1 increases vaginal tropoelastin production in vaginal SMC.
  International Urogynecology Journal 11/2011; 23(3):357-63. · 1.83 Impact Factor
• Article: Cellular proliferation in female pelvic organ prolapse: a pilot study.

  Peter Takacs, Cristina Saiz, Sindy Jaramillo, Yanping Zhang, Sujata Yavagal, Keith Candiotti, Carlos A Medina
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  ABSTRACT: To assess cell proliferation in pelvic organ prolapse (POP). Tissue samples of the anterior vaginal wall and uterosacral ligaments (USLs) were obtained from eight women with combined anterior vaginal wall and uterine prolapse and from eight women without POP in a standardized fashion. Immunohistochemistry against Ki-67 was used to assess cell proliferation in vaginal and USL biopsies. There were no significant differences in age, parity, menopausal status or hormone replacement therapy between the two groups. The POP-Q stage of uterine and anterior vaginal wall prolapse was significantly higher in the group of women with prolapse compared to the group without prolapse [median (range) 3 (3-4) vs. 0 (0), <0.01]. There was no significant difference between Ki-67 expressions in women with or without prolapse. There were no significant differences in cell proliferation between samples from women with or without POP.
  Archives of Gynecology 06/2011; 283(6):1329-32. · 0.91 Impact Factor
• Article: The effect of biological and synthetic meshes on vaginal smooth muscle cell proliferation.

  Marc Gualtieri, Yanping Zhang, Keith Candiotti, Sujata Yavagal, Carlos A Medina, Peter Takacs
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  ABSTRACT: To compare the effects of polypropylene mesh or porcine dermal acellular collagen matrix mesh with and without estradiol supplementation on vaginal smooth muscle cells (VaSMC) proliferation. Under in vitro culture conditions, VaSMC proliferation was significantly higher in the porcine dermal acellular collagen matrix mesh exposed cells compared to the type I polypropylene mesh exposed cells (relative cell number, mean ± SD, 0.27 ± 0.03 vs. 0.21 ± 0.01, P = 0.03). Under estradiol supplementation cell proliferation in the porcine mesh exposed cells remained significantly higher compared to the polypropylene mesh exposed cells (relative cell number, mean ± SD, 0.27 ± 0.04 vs. 0.15 ± 0.03, P < 0.01). The decreased rate of erosion found with the utilization of biological absorbable mesh in vaginal reconstructive surgery may partially be explained by the significantly increased VaSMC proliferation with porcine dermal acellular collagen mesh compared to polypropylene mesh.
  Neurourology and Urodynamics 02/2011; 30(3):435-7. · 2.96 Impact Factor
• Article: Levormeloxifene inhibits vaginal tropoelastin and transforming growth factor beta 1 production.

  Peter Takacs, Sujata Yavagal, Yanping Zhang, Keith Candiotti, Caros A Medina
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  ABSTRACT: To measure the effects of levormeloxifene on vaginal smooth muscle cell (SMC) proliferation, tropoelastin and transforming growth factor (TGF)-β1 production. Primary SMC cultures were performed from vaginal wall biopsies. SMC were incubated with levormeloxifene (0.1 µM, 1 µM), in 96-well plates and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumbromide (MTT) assay at 24 hours. Tropoelastin production was measured by the Fastin Assay kit and TGF-β1 levels were assessed by ELISA. SMC proliferation was significantly increased by levormeloxifene [relative cell number, mean ± SE, levormeloxifene 0.1 µM 130 ± 13% of control (P=NS), 1 µM 151 ± 19% of control (P<0.05)]. Tropoelastin production was significantly decreased by levormeloxifene [mean ± SE, levormeloxifene 0.1 µM 75 ± 4% of control (P=NS), 1 µM 64 ± 2% of control (P<0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, levormeloxifene 0.1 µM 79 ± 11% of control (P=NS), 1 µM 72 ± 14% of control (P<0.05)]. Levormeloxifene increases vaginal SMC proliferation, inhibits tropoelastin and TGF- β1 production.
  Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi. 01/2011; 47(1):11-9.
• Article: The effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation.

  Peter Takacs, Yanping Zhang, Sindy Jaramillo, Tarek Bardawil, Keith Candiotti, Carlos A Medina
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  ABSTRACT: To measure the effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation. Primary smooth muscle cell (SMC) cultures were performed from vaginal biopsies which were then incubated with estradiol (0.1 microM, 1 microM, 10 microM), progesterone (5 nM, 50 nM, 0.5 microM), or polypropylene mesh to assess cell proliferation. In vitro vaginal SMC proliferation was significantly increased by estradiol but not by progesterone or by polypropylene mesh (relative cell number, mean +/- SD, control vs. estrogen 0.24 +/- 0.02 vs. 0.28 +/- 0.02, P=0.01; control vs. progesterone 0.24 +/- 0.02 vs. 0.23 +/- 0.02, P=NS and control vs. mesh, 0.24 +/- 0.02 vs. 0.24 +/- 0.01, P=NS). In addition, estradiol increased cell proliferation in a dose responsive fashion (estradiol dose: 0.1 microM, 1 microM, 10 microM) compared to control (P=0.01). Vaginal SMC proliferation is significantly increased by estrogen.
  Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi. 01/2010; 46(1):9-15.
• Article: The impact of CYP2D6 genetic polymorphisms on postoperative morphine consumption.

  Keith A Candiotti, Zongqi Yang, Yiliam Rodriguez, Andres Crescimone, Greys C Sanchez, Peter Takacs, Carlos Medina, Yanping Zhang, Huanliang Liu, Melvin C Gitlin
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  ABSTRACT: Endogenous morphine-like compounds have been identified in humans and are released in response to stress. Human monocytes and granulocytes express the micro opiate receptor, micro3, which is morphine selective but opiate peptide insensitive. Recent studies have shown that CYP2D6 acts at critical steps for endogenous morphine biosynthesis. We theorized that ultrarapid (UM) CYP2D6 metabolizers may have an enhancement of their endogenous pain modulating mechanisms. After institutional review board approval, a previously developed surgical patient database was evaluated for information concerning CYP2D6 genotypes and morphine consumption. One hundred forty-two patients were found to have adequate information to be included in this current analysis. The study group was divided, based on morphine consumption, into two subgroups: low morphine consumers (LMC) (< or =10 mg/4 h, N = 80) and high morphine consumers (HMC) (>10 mg/4 h, N = 62). DNA was extracted from blood in all patients and was genotyped by the Amplichip (Roche, Pleasanton, CA) to determine the specific CYP2D6 genotypes. CYP2D6 UM were found to occur more frequently in the LMC group than in the HMC group (8/80 vs 0/62, P = 0.0091). No significant differences were noted for the poor, intermediate, or extensive metabolizers. Our current results suggest that CYP2D6 UM appear to require less morphine in the acute postoperative period compared with other CYP2D6 metabolizer groups. One possible mechanism for this observation is that CYP2D6 UM may have higher efficiency in synthesizing endogenous morphine compared with other metabolizers, thus increasing endogenous pain modulation and reducing the need for exogenous morphine.
  Pain Medicine 07/2009; 10(5):799-805. · 2.35 Impact Factor
• Article: The Effects of PPARδ Agonist and Zinc on Ovariectomized Rats' Vagina.

  Peter Takacs, Sindy Jaramillo, Yanping Zhang, Ram Datar, Anthony Williams, Joseph Olczyk, Keith Candiotti, Carlos A Medina
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  ABSTRACT: This study aimed to measure the effects of peroxisome proliferator-activated receptor-δ (PPARδ) agonist GW501516 (GW) and zinc sulfate (ZS) on ovariectomized rats' vaginal histomorphology and collagen expression. Two weeks after ovariectomy, rats received daily treatment with vaginal suppositories containing placebo, ZS, GW, ZS with GW, or estradiol for 2 weeks. Macroscopic measurements were taken and the midsection of the vagina was used for histology. Immunofluorescence was performed with antibodies against collagen I, III, and anti-actin or collagen I and V and anti-actin. Gene expression analysis of 3 collagen genes was performed by qRT-PCR. Macroscopic measurements revealed that the genital hiatus was narrower in the ZS and ZS with GW groups, and the vagina was significantly longer in the animals treated with GW, ZS with GW, and estradiol compared to the placebo group. Microscopic measurements of the vaginal layers showed that the lamina propria and the vaginal muscularis were significantly thicker in the ZS and ZS with GW group compared to the placebo.The ratio of vaginal Col1a1/Col3a1 mRNA expression was significantly up-regulated by ZS with GW compared to placebo, whereas the ratio of vaginal Col1a1/Col5a1 expression was significantly up-regulated by ZS, GW, and ZS with GW. The ratio of vaginal collagen I/III protein expression was significantly up-regulated by ZS and ZS with GW, whereas the ratio of vaginal collagen I/V expression was significantly up-regulated by estradiol, ZS, and ZS with GW compared to control. Vaginal suppositories containing zinc and PPARδ agonist significantly altered the vagina of ovariectomized rats.
  Journal of Pelvic Medicine and Surgery 19(3):126-31.