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Anabel Romero, Victoria González,
Anna Esteve,
Elisa Martró,
Lurdes Matas,
Cristina Tural,
Tomàs Pumarola,
Aurora Casanova,
Elena Ferrer,
Estrella Caballero, [......],
Carlos Alonso Villaverde,
Miguel Ángel Benítez,
Isabel Garcia,
Olga Díaz,
Jasmina Becerra,
Rosa Ros,
Roser Sala,
Isabel Rodrigo,
José M Miró,
Jordi Casabona
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ABSTRACT: BACKGROUND: Quantification and description of patients recently infected by HIV can provide an accurate estimate of the dynamics of HIV transmission. Between 2006 and 2008 in Catalonia, we estimated the prevalence of recent HIV infection among newly diagnosed cases, described the epidemiological characteristics of the infection according to whether it was recent, long-standing or advanced, and identified factors associated with recent infection. METHODS: A Test for Recent Infection (TRI) was performed in serum samples from patients newly diagnosed with HIV. Two different TRI were used: the Vironostika-LS assay (January 2006-May 2007) and the BED-CEIA CEIA (June 2007 onwards). Samples were obtained within the first 6 months of diagnosis. Patients whose samples tested positive in the TRI were considered recently infected. RESULTS: Of 1125 newly diagnosed patients, 79.9% were men (median age, 35.4 years), 38.7% were born outside Spain, 48.9% were men who have sex with men (MSM) and 10.6% presented other sexually transmitted infections. The overall percentage of recent infection was 23.0%, which increased significantly, from 18.1% in 2006 to 26.2% in 2008. This percentage was higher for patients from South America (27.6%). Factors associated with recent infection were acquiring infection through sexual contact between MSM [odds ratio (OR) 2.0; 95% confidence interval (95% CI) 1.1-3.9], compared with acquiring infection through heterosexual relations and being under 30 years of age (OR 5.9; 95% CI 1.9-17.4), compared with being over 50 years of age. CONCLUSION: The highest percentage of recent infection was identified in MSM, suggesting either a higher incidence or a greater frequency of HIV testing. Information regarding testing patterns is necessary to correctly interpret data from recently infected individuals. Systems to monitor the HIV epidemic should include both parameters.
The European Journal of Public Health 12/2011; · 2.73 Impact Factor
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ABSTRACT: To estimate the prevalence of direct and indirect syringe sharing among intravenous drug users (IDUs) attending a harm reduction center in Catalonia (Spain) and to identify factors associated with risk behaviors.
A cross-sectional study was conducted between 2008 and 2009 in harm reduction centers. Behavioral data were collected using anonymous questionnaires administered by trained interviewers.
Of the 748 respondents, 31.5% had shared syringes at least once in the previous 6 months and 55.2% reported sharing injection paraphernalia (spoons, water, filters). A higher risk of syringe sharing was found among IDUs who injected daily (OR=1.5), injected cocaine (OR=1.6), had less than half their supply of syringes from a free source (OR=2.5), had an IDU sexual partner (OR=1.8) or who reported indirect sharing (OR=4.1). A higher risk of indirect sharing was found in respondents who had an illegal source of income (OR=1.5), injected daily (OR=1, 5), injected cocaine (OR=1.4), reported sharing syringes (OR=3.9), or who reported a previous overdose (OR=1.5).
Despite the widespread use of harm reduction programs in Catalonia, a significant proportion of IDUs continue to practise injection-related risk behaviors. Further reductions in risk behaviors could be achieved by improving access to all sterile injecting equipment, especially among cocaine injectors and IDUs who inject frequently, and by including IDU sexual partners within the current network of harm reduction centers.
Gaceta Sanitaria 11/2011; 26(1):37-44. · 1.33 Impact Factor
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Anabel Romero,
Omar Sued,
Teresa Puig,
Anna Esteve,
Tomàs Pumarola,
Jordi Casabona, Victoria González,
Lurdes Matas,
Cristina Tural,
Isabel Rodrigo, [......],
Amat Ortí,
Rosa Navarro,
Josep M Euras,
Josep Vilaró,
M Carme Villà,
Santiago Montull,
Conrad Vilanova,
Ferran Pujol,
Olga Díaz,
Jose M Miró
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ABSTRACT: The objectives of this study were to assess the prevalence of transmitted HIV-1 drug resistances (TDR) and HIV-1 subtypes in recently infected patients in Catalonia between 2003 and 2005 and to describe the characteristics of these patients according to the presence or absence of TDR and HIV-1 subtype.
After application of the Serological Testing Algorithm for Recent HIV Seroconversion (STARHS), residual aliquots of serum samples from recently infected antiretroviral-naïve individuals were genotyped. FASTA sequences were analyzed using the HIVDB Program. The World Health Organization 2009 List of Mutations for Surveillance of Transmitted HIV-1 Drug Resistant HIV Strains was used to estimate the prevalence of TDR.
Of 182 recently infected patients, 14 (7.7%) presented TDR. Seven (3.8%) had genotypic evidence of TDR against non-nucleoside reverse transcriptase inhibitors, 6 (3.3%) against nucleoside reverse transcriptase inhibitors, 3 (1.6%) against protease inhibitors (PIs), and only 2 individuals (1.1%) presented TDR against more than one class of drugs. Thirty-five (19.2%) patients were infected with a non-B HIV-1 subtype.
This is the first study to estimate the prevalence of TDR in recently infected patients in Catalonia. The results are similar to those of studies performed in other Spanish regions. Correct monitoring of these parameters requires systematic epidemiologic surveillance of transmitted resistance.
Enfermedades Infecciosas y Microbiología Clínica 05/2011; 29(7):482-9. · 1.49 Impact Factor
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Enfermedades Infecciosas y Microbiología Clínica 04/2011; 29(7):553-5. · 1.49 Impact Factor
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ABSTRACT: To describe trends in HIV prevalence, in risk behaviors associated with HIV transmission, and in knowledge and attitudes related to antiretroviral therapy (ART) among men who have sex with men (MSM) recruited in Catalonia between 1995 and 2006.
Biannual cross-sectional surveys were performed. An opportunistic sample of MSM was recruited in saunas, sex shops, bars and a cruising site in a public park. In addition, an anonymous self-administered questionnaire was sent by mail to all members of a gay and lesbian association (Coordinadora Gai-Lesbiana). Oral fluid samples were collected to determine HIV prevalence. Linear trends in proportions were assessed by the chi(2) test, stratified by age.
The overall prevalence of HIV infection increased from 8.5% (1995) to 14.1% (2006) among men aged less than 30 years old (p=0.162) and from 18.2% (1995) to 21.2% (2006) among those aged 30 years old or more (p=0.07). Consistent condom use in anal intercourse with occasional partners decreased from 72.9% to 58.7% in men aged less than 30 years old (p <0.05) and from 77.2% to 65.6% in those aged 30 years old or more (p<0.001). The proportion of men who reported they chose riskier behaviors because of ART increased from 9.2% to 19.4% in men aged less than 30 years old and from 8.3% to 16.7% in those aged 30 years old or more (p<0.05).
An increasing trend in the prevalence of HIV and associated risk behaviors was found in Catalonia among MSM. Therefore, preventive programs targeting this population should be intensified and should include new risk reduction strategies, as well as other educational messages about ART.
Gaceta Sanitaria 12/2009; 24(1):40-6. · 1.33 Impact Factor
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ABSTRACT: The objectives of this study were to estimate the prevalence of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) among injection drug users (IDUs) and identify the sociodemographic and behavioral factors in this population associated with these infections.
Cross-sectional study in IDUs recruited in Catalonia in 2006.
Ever-sharing syringes was associated with both HIV and HCV infection. Indirect sharing of injecting equipment and injecting cocaine as the main drug were factors associated with HCV infection, and the fact of having injected in prison was associated with HIV infection.
Identification of sociodemographic and behavioral factors associated with these infections can be of help when designing specific preventive interventions for IDUs.
Enfermedades Infecciosas y Microbiología Clínica 09/2009; 28(4):236-8. · 1.49 Impact Factor
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ABSTRACT: The increasing use of molecular methods strongly motivates clinical laboratories to introduce automated nucleic acid extractors. We compared the easyMAG (bioMérieux) with a manual extraction method for hepatitis C virus (HCV) load quantification (RealTime HCV; Abbott). Both methods were comparable, and, therefore, the easyMAG is suitable to be implemented in our laboratory for the management of HCV-infected patients.
Journal of clinical microbiology 02/2009; 47(3):811-3. · 4.16 Impact Factor
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A Romero, V González,
M Granell,
L Matas,
A Esteve,
E Martró,
I Rodrigo,
T Pumarola,
J M Miró,
A Casanova,
E Ferrer,
C Tural,
J del Romero,
C Rodríguez,
E Caballero,
E Ribera,
J Casabona
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ABSTRACT: Identification of recent HIV infections provides a description of the current pattern of HIV transmission and, consequently, can help to design better preventive interventions. Our study shows the first implementation in Spain of the Serologic Testing Algorithm for Recent HIV Seroconversion (STARHS) strategy. We assess the viability of introducing STARHS in our setting and describe the frequency and epidemiological characteristics of recent infections (RIs).
Between 2003 and 2005, HIV-positive blood samples drawn for diagnostic purposes were collected from 28 Spanish laboratories to be tested using STARHS. Samples from patients with a previous HIV diagnosis, age <18 years, <200 CD4 cells/microL or clinical AIDS criteria were excluded from the analysis.
A total of 660 (19.2%) samples were classified as RI. Most people identified with RI were male (79.8%) with a median age of 33.1 years, and 62.5% occurred among men who have sex with men (MSM). Immigrants made up 26.5% of individuals identified with RIs, with 48.7% coming from South America. Among the individuals with RI, at least 16.5% had reported another sexually transmitted infection (STI) during the year before the HIV diagnosis.
The study shows that the implementation of STARHS in our setting is feasible and has highlighted important features of the local HIV epidemic, such as the ongoing spread of HIV among MSM, the potential role of STIs in RIs and the vulnerability of immigrants as a new target population.
Sexually transmitted infections 12/2008; 85(2):106-10. · 2.18 Impact Factor
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ABSTRACT: We report the evaluation of a new real-time PCR assay for hepatitis C virus (HCV) genotyping. The assay design is such that genotype 1 isolates are typed by amplification targeting the nonstructural 5b (NS5b) subgenomic region. Non-genotype 1 isolates are typed by type-specific amplicon detection in the 5' noncoding region (5'NC) (method 1; HCV genotyping analyte-specific reagent assay). This method was compared with 5'NC reverse hybridization (method 2; InnoLiPA HCV II) and 5'NC sequencing (method 3; Trugene HCV 5'NC). Two hundred ninety-five sera were tested by method 1; 223 of them were also typed by method 2 and 89 by method 3. Sequencing and phylogenetic analysis of an NS5b fragment were used to resolve discrepant results. Suspected multiple-genotype infections were confirmed by PCR cloning and pyrosequencing. Even though a 2% rate of indeterminates was obtained with method 1, concordance at the genotype level with results with methods 2 and 3 was high. Among eight discordant results, five mixed infections were confirmed. Genotype 1 subtyping efficiencies were 100%, 77%, and 74% for methods 1, 2, and 3, respectively; there were 11/101 discordants between methods 1 and 2 (method 1 was predominantly correct) and 2/34 between methods 2 and 3. Regarding genotype 2, subtyping efficiencies were 100%, 45%, and 92% by methods 1, 2, and 3, respectively; NS5b sequencing of discordants (16/17) revealed a putative new subtype within genotype 2 and that most subtype calls were not correct. Although only sequencing-based methods provide the possibility of identifying new variants, the real-time PCR method is rapid, straightforward, and simple to interpret, thus providing a good single-step alternative to more-time-consuming assays.
Journal of clinical microbiology 02/2008; 46(1):192-7. · 4.16 Impact Factor
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ABSTRACT: A study was performed to diagnose tuberculosis by smear, culture, and nucleic acid amplification. The study was comprised of two independent arms. Each arm used a different specimen processing method; in one arm, all specimens were processed with N-acetyl-l-cysteine-sodium hydroxide, and in the other arm, all specimens were processed with C(18)-carboxypropylbetaine and lytic enzymes. In each arm, all processed sediments were split for analysis by auramine smear, by culture using the MB/BacT liquid culture system and solid media, and by nucleic acid amplification using the COBAS AMPLICOR MTB test. In the N-acetyl-l-cysteine-sodium hydroxide arm, 1,468 specimens were analyzed: 65 were smear positive; 88 and 42 were culture positive for Mycobacterium tuberculosis and nontuberculous mycobacteria, respectively; and 103 were PCR positive. Relative to cultures positive for M. tuberculosis, the sensitivity and specificity of the smear were 68.2% and 99.6%, respectively, and those of PCR were 75.0% and 97.3%, respectively. In the C(18)-carboxypropylbetaine study arm, 1,423 specimens were analyzed: 44 were smear positive; 82 and 31 were culture positive for M. tuberculosis and nontuberculous mycobacteria, respectively; and 91 were PCR positive. The sensitivity and specificity of the smear were 48.8% and 99.7%, respectively, and those of PCR were 78.0% and 98.0%, respectively. When the two arms were compared, C(18)-carboxypropylbetaine specimen processing significantly increased the number of smear-negative and culture-positive specimens and significantly increased the PCR sensitivity among this same group of specimens while at the same time significantly reducing the inhibition rate.
Journal of Clinical Microbiology 01/2006; 43(12):6091-7. · 4.15 Impact Factor
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ABSTRACT: A study was designed to compare an avidity index method to the serologic testing algorithm for recent human immunodeficiency virus (HIV) seroconversion (STARHS) for the detection of recent HIV infection. One hundred sixty HIV-positive sera were tested. Both techniques performed similarly in identifying recent infections, although STARHS tended to misclassify more individuals that had long-standing infection as being recently infected.
Journal of Clinical Microbiology 01/2006; 43(12):6197-9. · 4.15 Impact Factor
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Eduardo Padilla, Victoria González,
Jose María Manterola,
Andrés Pérez,
María Dolores Quesada,
Sergio Gordillo,
Cristina Vilaplana,
María Angeles Pallarés,
Sonia Molinos,
María Dolores Sánchez,
Vicente Ausina
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ABSTRACT: The performance of two DNA line probe assays, a new version of INNO-LiPA Mycobacteria (Innogenetics, Ghent, Belgium) and the GenoType Mycobacterium (Hain Diagnostika, Nehren, Germany), were evaluated for identification of mycobacterial species isolated from liquid cultures. Both tests are based on a PCR technique and designed for simultaneous identification of different mycobacterial species by reverse hybridization and line probe technology. The INNO-LiPA Mycobacteria v2 targeting the 16S-23S rRNA gene spacer region was developed for the simultaneous identification of 16 different mycobacterial species. The GenoType Mycobacterium, which targets the 23S rRNA gene, allows simultaneous identification of 13 mycobacterial species. Both tests were evaluated on 110 mycobacterial strains belonging to 22 different mycobacterial species (20 reference strains, 83 clinical strains, and 4 Mycobacterium kansasii strains isolated from tap water) that were previously inoculated into MB/BacT bottles. The sensitivity of both methods, defined as the number of positive results obtained with the Mycobacterium genus probe together with an interpretable result on the number of samples tested was 110 of 110 (100%) for INNO-LiPA and 102 of 110 (92.7%) for GenoType. For samples with interpretable results, INNO-LiPA was able to correctly identify 109 of 110 samples (99.1%), whereas the GenoType correctly identified 100 of 102 samples (98.0%). Both tests were easy to perform, rapid, and reliable when applied to mycobacterial identification directly from MB/BacT bottles.
Journal of Clinical Microbiology 08/2004; 42(7):3083-8. · 4.15 Impact Factor
