Guanzhou Qiu

State Key Laboratory of Medical Genetics of China, Ch’ang-sha-shih, Hunan, China

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Publications (192)458.78 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Sulfate adenylyltransferase gene and 4Fe-4S ferredoxin gene are the key genes related to sulfur and iron oxidations during bioleaching system, respectively. In order to better understand the bioleaching and microorganism synergistic mechanism in chalcopyrite bioleaching by mixed culture of moderate thermophiles, expressions of the two energy metabolism genes and community dynamics of free and attached microorganisms were investigated. Specific primers were designed for real-time quantitative PCR to study the expression of these genes. Real-time PCR results showed that sulfate adenylyltransferase gene was more highly expressed in Sulfobacillus thermosulfidooxidans than that in Acidithiobacillus caldus, and expression of 4Fe-4S ferredoxin gene was higher in Ferroplasma thermophilum than that in S. thermosulfidooxidans and Leptospirillum ferriphilum. The results indicated that in the bioleaching system of chalcopyrite concentrate, sulfur and iron oxidations were mainly performed by S. thermosulfidooxidans and F. thermophilum, respectively. The community dynamics results revealed that S. thermosulfidooxidans took up the largest proportion during the whole period, followed by F. thermophilum, A. caldus, and L. ferriphilum. The CCA analysis showed that 4Fe-4S ferredoxin gene expression was mainly affected (positively correlated) by high pH and elevated concentration of ferrous ion, while no factor was observed to prominently influence the expression of sulfate adenylyltransferase gene.
    Current Microbiology 05/2015; 71(1). DOI:10.1007/s00284-015-0817-7
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    ABSTRACT: Needle-like CoO nanowires grown on carbon cloth have been successfully fabricated by a controllable hydrothermal method followed via annealing process. The as-fabricated nanostructure showed enhanced specific capacitances and excellent cycling stability in supercapacitors.
    RSC Advances 04/2015; 5(52). DOI:10.1039/C5RA01364J
  • Jun Wang, Hongbo Zhao, Wenqing Qin, Guanzhou Qiu
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    ABSTRACT: Double-shell cobalt monoxide (CoO) hollow particles were successfully synthesized by a facile and effective one-pot solution-based synthetic route. The inner architecture and outer structure of the double-shell CoO hollow particles could be readily created through controlling experimental parameters. A possible formation mechanism was proposed based on the experimental results. The current synthetic strategy has good prospects for the future production of other transition-metal oxides particles with hollow interior. Furthermore, double-shell cobalt oxide (Co3O4) hollow particles could also be obtained through calcinating corresponding CoO hollow particles. The magnetic measurements revealed double-shell CoO and Co3O4 hollow particles exhibit ferromagnetic and antiferromagnetic behaviour, respectively.
    Scientific Reports 03/2015; 5:8737. DOI:10.1038/srep08737
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    ABSTRACT: Hollow BiVO4 microspheres are prepared in the presence of ethylenediaminetetraacetic acid (EDTA) through a hydrothermal method, and used as a template for the fabrication of Ag-nanoparticle-decorated BiVO4 (BiVO4/Ag) composite microspheres. The morphology and structure are characterized by transmission electron microscopy and scanning electron microscopy. It is found that EDTA plays an important role in the formation of the hollow microspheres, and the possible formation mechanism is discussed. UV/visible spectrometry analysis shows that the BiVO4/Ag composite microspheres exhibit a distinct surface plasmon absorption band compared with pure BiVO4 microspheres. The photocatalytic activities of the BiVO4/Ag composite microspheres are examined by studying the degradation of rhodamine 6G (R6G) solutions under visible-light irradiation (>400 nm). The results show clearly that the BiVO4/Ag composite microspheres possess a much higher photocatalytic activity than pure BiVO4 microspheres and P25. In addition, Raman analyses show that the BiVO4/Ag composite microspheres exhibit an excellent surface-enhanced Raman scattering (SERS) performance.
    ChemPlusChem 02/2015; 80(5). DOI:10.1002/cplu.201402434
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    ABSTRACT: Thermodynamic calculations and electrochemical measurements were employed to establish a mathematic model for predicting the optimum redox potential in chalcopyrite leaching at 318 K. The proposed model revealed that the optimum redox potential mainly depended on the temperature as well as the concentrations of Cu2+ and Fe2+. Chemical leaching (ferric leaching) of chalcopyrite indicated that the proposed model was accurate, from which the optimum redox potential of bioleaching of chalcopyrite can also be accurately predicted. Based on the model, the redox potential should be maintained at an optimum potential range (E-L-E-H) to obtain a high leaching efficiency. Moreover, the addition of Cu2+ and Fe2+ accelerated the dissolution of chalcopyrite remarkably at the initial stage of bioleaching, but the further dissolution was inhibited owing to the jarosite formed during the bioleaching process. Therefore, Cu2+ and Fe2+ should be added periodically to prevent the rapid formation of jarosite and enhance the bioleaching efficiency. (C) 2014 Elsevier B.V. All rights reserved.
    Hydrometallurgy 01/2015; 151:141-150. DOI:10.1016/j.hydromet.2014.11.009
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    ABSTRACT: A bioleaching consortium including F. thermophilum improved copper extraction.•F. thermophilum introduction complicated free and attached population dynamics.•Cooperation between organisms weakened the inhibitory effects of stress conditions.•Synergetic acts of F. thermophilum increased cell growth and metabolic capacities.
    Biochemical Engineering Journal 01/2015; 93. DOI:10.1016/j.bej.2014.10.004
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    ABSTRACT: In this work, a new stirred reactor was designed and then a comparative study of bioleaching using stirred reactor and shake flask was carried out to investigate the bioleaching performance of the new stirred reactor. Results showed that variations of redox potentials and acid consumptions during were similar in the two systems, and about 15% more copper extraction can be obtained by stirred reactor. Bacterial concentration increased more sharply in the initial stage of bioleaching in stirred reactor. Analytical results showed that a worse passivation caused by larger amount of jarosite and elemental sulfur on the minerals surface leaded the lower copper extraction in shake flask. Electrochemical analysis revealed that the conductivity of bioleaching residues of shake flasks is lower than that of bioleaching residues of stirred reactor. Stirred reactor possesses significant advantages over shake flask in bioleaching of chalcopyrite concentrates can be caused mainly by better mass transfer effect, better air inflation effect and stronger shear force. Therefore, the stirred reactor can be used for both enlarge cultivation of bacteria and tank bioleaching of ores. Electrochemical measurements revealed that chalcopyrite dissolution was preferred to be a continuous reduction-oxidation pathway, in which chalcopyrite was initially reduced and then oxidized, and the initial reduction reaction was the rate-limiting step.
    International journal of electrochemical science 01/2015; 10:848-858.
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    ABSTRACT: The availability of sensitive, reproducible and stable substrate is critically important for surface enhanced Raman scattering (SERS)-based application, but it still remains a challenge up to now. In this work, urchin-like LaVO4 microspheres prepared by a hydrothermal method were used as a template to fabricate SERS substrate by deposition of Au nanoparticle onto the surfaces of LaVO4 microspheres. The coverage of Au nanoparticles on the surfaces of LaVO4 microspheres can be easily controlled by varying the amount of Au precursor. SERS measurement showed that the coverage of Au nanoparticles on the surfaces of LaVO4 microspheres had a great effect on SERS activity. The SERS signals collected from 80 microspheres indicated that as-prepared SERS substrate exhibited a good reproducibility. Detection of melamine molecules with a low concentration (1.0×10(-9)M) was used as an example to show the possible application of such substrate. In addition, the effect of iron ion (Fe(3+)) on detection melamine from the mixture of melamine and benzoic acid was also investigated. It was found that the interference of benzoic acid in detecting melamine from the mixture can be removed by adding Fe(3+). Copyright © 2014 Elsevier Inc. All rights reserved.
    Journal of Colloid and Interface Science 12/2014; 443C:80-87. DOI:10.1016/j.jcis.2014.12.011
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    ABSTRACT: This paper presents a study on the relation between bacterial adhesion force and bioleaching rate of chalcopyrite, which sheds light on the influence of interfacial interaction on bioleaching behavior. In our research, Acidithiobacillus ferrooxidans (A. ferrooxidans) were adapted to grow with FeSO4·7H2O, element sulfur or chalcopyrite. Then, surface properties of Acidithiobacillus ferrooxidans and chalcopyrite were analyzed by contact angle, zeta potential and Fourier transform infrared spectroscopy (FTIR). Adhesion force between bacteria and chalcopyrite was measured by atomic force microscopy (AFM). Attachment and bioleaching behaviors were also monitored. The results showed that A. ferrooxidans adapted with chalcopyrite exhibited the strongest adhesion force to chalcopyrite and the highest bioleaching rate. Culture adapted with sulfur bacteria took second place and FeSO4·7H2O-adapted bacteria were the lowest. Bioleaching rate and bacterial attachment capacity were positively related to bacterial adhesion force, which is affected by the nature of energy source. According to this work, the attachment of bacteria to chalcopyrite surface is one of the most important aspects that influence the bioleaching process of chalcopyrite. Copyright © 2014 Elsevier B.V. All rights reserved.
    Colloids and surfaces B: Biointerfaces 11/2014; 126. DOI:10.1016/j.colsurfb.2014.11.036
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    ABSTRACT: The dissolution process of chalcopyrite in the presence of mesophilic microorganisms was investigated by electrochemical measurements. X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) analysis, accompanied by leaching experiments. Results proved that the presence of Acidithiobacillus ferrooxidans enhanced the initial reduction of chalcopyrite to intermediate species (Cu2S), thus promoting the dissolution of chalcopyrite in the initial stage of bioleaching. However, chalcopyrite tended to be directly oxidized to polysulfide (Sn2−) and CuS in the later stage of bioleaching when redox potential was higher than 0.5 V (vs. Ag/AgCl), the formed polysulfide and jarosite can be responsible for the passivation of chalcopyrite in the later stage of bioleaching by A.ferrooxidans. On the contrary, Chalcopyrite was mainly directly oxidized to polysulfide and CuS in the presence of sterile 9K medium or Acidithiobacillus thiooxidans, and the initial reduction reaction was still the rate-limiting step, thus resulting in low copper extraction rate.
    Minerals Engineering 11/2014; 71. DOI:10.1016/j.mineng.2014.10.025
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    ABSTRACT: Although there were lots of methods focused on DNA and RNA extraction, only few studies focused on simultaneous extraction of high quality DNA and RNA from environmental samples, especially for acidic habitat samples. In this study, a widely used DNA extraction method (Zhou, J. Z., M. A. Bruns, and J. M. Tiedje. 1996) was optimized for extraction of crude nucleic acid (including DNA and RNA) simultaneously, and salt based step-by-step precipitation method was also optimized for separation of DNA and RNA. The results showed that nucleic acid extraction buffer (pH 7.0) containing piperazine-1,4-bis(2-ethanesulfonic acid)(PIPES) salt could extract crude nucleic acid from acidic habitat samples effectively and obtained nucleic acid could be separated to pure DNA and RNA by the optimized step-by-step precipitation method using saturated LiCl solution. The results also showed that obtained DNA and RNA with high quality and could be used for the downstream molecular analysis. This study developed a useful method for simultaneous extraction and separation of high quality DNA and RNA from acidic habitat samples, which has a great potential as a standard method to obtain comparable data for molecular biological analysis of biohydrometallurgy and extreme environmental samples.
    Analytical methods 11/2014; 7(3). DOI:10.1039/C4AY01608D
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    ABSTRACT: The semiconductor nanostructures decorated with noble metals have attracted increasing attention due to their interesting physical and chemical properties. In this work, urchin-like monoclinic (m-) LaVO4 microspheres were prepared by a hydrothermal method and used as a template to fabricate Ag nanoparticle-decorated m-LaVO4 composites. The morphology and structure were characterized by transmission electron microscope, high-resolution transmission electron microscope, scanning electron microscope, and energy-dispersive X-ray. It was found that Ag nanoparticles with narrow size distribution were uniformly loaded on urchin-like m-LaVO4 microspheres, and the resulted composite microspheres showed distinct surface plasmon absorption band compared to pure m-LaVO4 microspheres. Photocatalytic activities of as-prepared samples were examined by studying the degradation of methyl orange solutions under visible-light irradiation (> 400 nm). Results clearly showed that urchin-like m-LaVO4/Ag microspheres possess much higher photocatalytic activity than pure m-LaVO4 microspheres and P25.
    Materials Characterization 10/2014; 98. DOI:10.1016/j.matchar.2014.10.021
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    ABSTRACT: The layered structure of the raw pyrophyllite (pattern a) is collapsed thoroughly when calcined over 1100 °C, accompanied by the formation of amorphous SiO2 and poorly ordered mullite (pattern b). The amorphous SiO2 in the activated pyrophyllite is soluble in caustic soda solution, but mullite is insoluble (pattern c). Therefore, silicon can be removed from pyrophyllite by TCA process followed by alkali leaching. The main reactions include: Thermochemical activation of silicon of pyrophyllite:Al2O3·4SiO2 → 3Al2O3·2SiO2 + SiO2(amorphous) Dissolving of amorphous SiO2 of activated pyrophyllite:SiO2(amorphous) + NaOH + aq → Na2SiO3 + H2O + aq Precipitation reaction during alkali leaching:Na2SiO3 + NaAl(OH) 4+ aq → NaOH + 0.95Na2O·Al2O3·3.25SiO2·4.79H2O↓ + H2O + aq
    Applied Clay Science 09/2014; 99:282–288. DOI:10.1016/j.clay.2014.07.011
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    ABSTRACT: Nickel foam supported Zn-Co hydroxide nanoflakes were fabricated by a facile solvothermal method. Benefited from the unique structure of Zn-Co hydroxide nanoflakes on a nickel foam substrate, the as prepared materials exhibited an excellent specific capacitance of 901 F g(-1) at 5 A g(-1) and remarkable cycling stability as electrode materials in supercapacitors.
    Chemical Communications 08/2014; 50(76). DOI:10.1039/c4cc05057f
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    ABSTRACT: A moderately thermophilic culture was used to bioleach chalcopyrite. It showed a good performance in copper extraction. In order to further improve efficiency of chalcopyrite bioleaching, effects of pH control and redox potential (ORP) control on chalcopyrite dissolution were investigated. The community dynamics of planktonic and attached populations were also monitored during bioleaching of chalcopyrite at different conditions. The copper extraction was improved by controlling pH or ORP, especially in the final stage of the bioleaching. The maximal growth rate of microorganisms was up to 0.94 generations/day when the pH was controlled in the range of 1.40-1.85. The ORP controlled at 420 ± 20 mV caused a reduced jarosite formation. Community dynamics analyses show that the pH control and the ORP control had significant effects on community dynamics of planktonic and attached moderate thermophiles. The species contained in the culture showed different succession trends compared with each other, not only in the leachate but also on the mineral surfaces. The pH control was not favorable for the attachment of microorganisms. It can also be found that succession of attached cells is significantly different from the community dynamics for their planktonic counterparts.
    Hydrometallurgy 08/2014; 147. DOI:10.1016/j.hydromet.2014.04.013
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    ABSTRACT: Synthetic rutile was prepared by treating Panzhihua titanium slag with H3PO4 followed by leaching. To this end, the effects of oxidation temperature and H3PO4 dosage on the phase transformation of titanium slag were studied by XRD, while the microstructure and composition of the treated slag were characterized by SEM and EDAX. It was determined that roasting with H3PO4 promoted the transformation of titanium slag into rutile. Furthermore, hydrochloric acid leaching of treated titanium slag was investigated in terms of the effect of HCl concentration, and leaching temperature, time and liquid-to-solid mass ratio on leaching. The results show that Fe, Mg, Ca and Al can be effectively removed by leaching with 20 wt.% HCl at a liquids-to-solids ratio of 5:1 at 110 °C for 2 h. It was found that a NaOH leaching step is necessary to remove Si and P to improve the purity of synthetic rutile. Using this process, synthetic rutile containing 95.34% TiO2 and 0.37% (MgO + CaO) was obtained.
    Hydrometallurgy 08/2014; 147. DOI:10.1016/j.hydromet.2014.05.009
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    ABSTRACT: Metal resistance of acidophilic bacteria is very significant during bioleaching of copper ores since high concentration of metal is harmful to the growth of microorganisms. The resistance levels of six Acidithiobacillus ferrooxidans strains to 0.15 M copper and 0.2 M zinc were investigated, and eight metal resistance-related genes (afe-0022, afe-0326, afe-0329, afe-1143, afe-0602, afe-0603, afe-0604, and afe-1788) were sequenced and analyzed. The transcriptional expression levels of eight possible metal tolerance genes in six A. ferrooxidans strains exposed to 0.15 M Cu(2+) and 0.2 M Zn(2+) were determined by real-time quantitative PCR (RT-qPCR), respectively. The copper resistance levels of six A. ferrooxidans strains declined followed by DY26, DX5, DY15, GD-B, GD-0, and YTW. The zinc tolerance levels of six A. ferrooxidans strains exposed to 0.2 M Zn(2+) from high to low were YTW > GD-B > DY26 > GD-0 > DX5 > DY15. Seven metal tolerance-related genes all presented in the genome of six strains, except afe-0604. The metal resistance-related genes showed different transcriptional expression patterns in six A. ferrooxidans strains. The expression of gene afe-0326 and afe-0022 in six A. ferrooxidans strains in response to 0.15 M Cu(2+) showed the same trend with the resistance levels. The expression levels of genes afe-0602, afe-0603, afe-0604, and afe-1788 in six strains response to 0.2 M Zn(2+) did not show a clear correlation between the zinc tolerance levels of six strains. According to the results of RT-qPCR and bioinformatics analysis, the proteins encoded by afe-0022, afe-0326, afe-0329, and afe-1143 were related to Cu(2+) transport of A. ferrooxidans strains.
    Current Microbiology 07/2014; 69(6). DOI:10.1007/s00284-014-0652-2
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    ABSTRACT: The genus Sulfobacillus is a cohort of mildly thermophilic or thermotolerant acidophiles within the phylum Firmicutes and requires extremely acidic environments and hypersalinity for optimal growth. However, our understanding of them is still preliminary partly because few genome sequences are available. Here, the draft genome of Sulfobacillus thermosulfidooxidans strain ST was deciphered to obtain a comprehensive insight into the genetic content and to understand the cellular mechanisms necessary for its survival. Furthermore, the expressions of key genes related with iron and sulfur oxidation were verified by semi-quantitative RT-PCR analysis. The draft genome sequence of Sulfobacillus thermosulfidooxidans strain ST, which encodes 3225 predicted coding genes on a total length of 3,333,554 bp and a 48.35% G+C, revealed the high degree of heterogeneity with other Sulfobacillus species. The presence of numerous transposases, genomic islands and complete CRISPR/Cas defence systems testifies to its dynamic evolution consistent with the genome heterogeneity. As expected, S. thermosulfidooxidans encodes a suit of conserved enzymes required for the oxidation of inorganic sulfur compounds (ISCs). The model of sulfur oxidation in S. thermosulfidooxidans was proposed, which showed some different characteristics from the sulfur oxidation of Gram-negative A. ferrooxidans. Sulfur oxygenase reductase and heterodisulfide reductase were suggested to play important roles in the sulfur oxidation. Although the iron oxidation ability was observed, some key proteins cannot be identified in S. thermosulfidooxidans. Unexpectedly, a predicted sulfocyanin is proposed to transfer electrons in the iron oxidation. Furthermore, its carbon metabolism is rather flexible, can perform the transformation of pentose through the oxidative and non-oxidative pentose phosphate pathways and has the ability to take up small organic compounds. It encodes a multitude of heavy metal resistance systems to adapt the heavy metal-containing environments.
    PLoS ONE 06/2014; 9(6):e99417. DOI:10.1371/journal.pone.0099417
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    ABSTRACT: A facile hydrothermal route followed by annealing was applied for the preparation of monoclinic yttrium oxysulfate hollow spheres with other rare earth ions (Yb3+ and Er3+) doped. The formation of hollow spheres may involve the Ostwald ripening. The hybrid materials were demonstrated as an example for upconversion application. The compatibility of host crystal structure enables the easy doping of a second rare-earth metal element without significantly changing the host lattice. The luminescent properties were affected by the ratio and concentration of dopant rare earth metal ions due to the featured energy transfer and symmetry of crystal field. The type of luminescent center and the crystallinity of samples were also proved to have remarkable influences on the optical properties of as-prepared products.
    Nano Research 06/2014; 7(8):1093-1102. DOI:10.1007/s12274-014-0472-5

Publication Stats

2k Citations
458.78 Total Impact Points


  • 2008–2015
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China
  • 2002–2015
    • Central South University
      • • School of Minerals Processing and Bioengineering
      • • Department of Bioengineering
      Ch’ang-sha-shih, Hunan, China
  • 2014
    • South Central College
      Central, Louisiana, United States
  • 2011
    • University of Oklahoma
      • Institute for Environmental Genomics
      Norman, OK, United States
  • 2006
    • Peking University
      Peping, Beijing, China
  • 2003
    • University of Utah
      • Department of Metallurgical Engineering
      Salt Lake City, Utah, United States
  • 1997–1998
    • Central South University of Forestry and Technology
      Ch’ang-sha-shih, Hunan, China