Zuguo Liu

Xiamen University, Amoy, Fujian, China

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Publications (76)187.67 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Periostin is a non-structural matricellular protein. Little is known about periostin in human limbal stem cells (LSCs). This study was to explore the unique expression pattern and functional role of periostin in maintaining the properties of human LSCs. Fresh donor corneal tissues were used to make cryosections for evaluation of periostin expression on ex vivo tissues. Primary human limbal epithelial cells (HLECs) were generated from limbal explant culture. In vitro culture models for proliferation and epithelial regeneration were performed to explore functional role of periostin in LSCs. The mRNA expression was determined by reverse transcription and quantitative real-time PCR (RT-qPCR), and the protein production and localization were detected by immunofluorescent staining and Western blot analysis. Periostin protein was found to be exclusively immunolocalized in the basal layer of human limbal epithelium. Periostin localization was well matched with nuclear factor p63, but not with corneal epithelial differentiation marker Keratin 3. Periostin transcripts was also highly expressed in limbal than corneal epithelium. In primary HLECs, periostin expression at mRNA and protein levels was significantly higher in 50% and 70% confluent cultures at exponential growth stage than in 100% confluent cultures at slow growth or quiescent condition. This expression pattern was similar to other stem/progenitor cell markers (p63, integrin β1 and TCF4). Periostin expression at transcripts, protein and immunoreactivity levels increased significantly during epithelial regeneration in wound healing process, especially in 16-24 hours at wound edge, which was accompanied by similar upregulation and activation of p63, integrin β1 and TCF4. Our findings demonstrated that periostin is exclusively produced by limbal basal epithelium and co-localized with p63, where limbal stem cells reside. Periostin promotes HLEC proliferation and regeneration with accompanied activation of stem/progenitor cell markers p63, integrin β1 and TCF4, suggesting its novel role in maintaining the phenotype and functional properties of LSC.
    PLoS ONE 02/2015; 10(2):e0117139. DOI:10.1371/journal.pone.0117139 · 3.53 Impact Factor
  • Ophthalmology 01/2015; 122(1):e4. DOI:10.1016/j.ophtha.2014.07.013 · 6.17 Impact Factor
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    ABSTRACT: to test the effectiveness of breast feeding (BF), music therapy (MT), and combined breast feeding and music therapy (BF+MT) on pain relief in healthy-term neonates during heel lance.Designrandomised controlled trial.Settingin the postpartum unit of one university-affiliated hospital in China from August 2013 to February 2014.Participantsamong 288 healthy-term neonates recruited, 250 completed the trial. All neonates were undergoing heel lancing for metabolic screening, were breast fed, and had not been fed for the previous 30 minutes.Interventionsall participants were randomly assigned into four groups – BF, MT, BF+MT, and no intervention – with 72 neonates in each group. Neonates in the control group received routine care. Neonates in the other three intervention groups received corresponding interventions five minutes before the heel lancing and throughout the whole procedure.MeasurementsNeonatal Infant Pain Scale (NIPS), latency to first cry, and duration of first crying.Findingsmean changes in NIPS scores from baseline over time was dependent on the interventions given. Neonates in the BF and combined BF+MT groups had significantly longer latency to first cry, shorter duration of first crying, and lower pain mean score during and one minute after heel lance, compared to the other two groups. No significant difference in pain response was found between BF groups with or without music therapy. The MT group did not achieve a significantly reduced pain response in all outcome measures.ConclusionsBF could significantly reduce pain response in healthy-term neonates during heel lance. MT did not enhance the effect of pain relief of BF.Implications for practicehealthy-term neonates should be breast fed to alleviate pain during heel lance. There is no need for the additional input of classical music on breast feeding in clinic to relieve procedural pain. Nurses should encourage breast feeding to relieve pain during heel lance.
    Midwifery 11/2014; DOI:10.1016/j.midw.2014.11.001 · 1.71 Impact Factor
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    ABSTRACT: Purpose: Using anterior chamber optical coherence tomography to evaluate changes in angle anatomy in patients with primary angle-closure glaucoma (PACG) before and after trabeculectomy. Methods: This is a prospective observational study in 38 eyes of 38 patients with PACG, who underwent trabeculectomy. We used customized software to analyze optical coherence tomography images (Visante) of all participants before and after the surgical treatment. Multivariate linear regression analysis was performed for predictors of percentage change in mean angle parameters, including scleral spur angle, angle opening distance, angle recess area, and trabecular-iris surface area. The main outcome measures were changes in angle parameters and their relationship with age, sex, refraction, MD, visual outcomes, central corneal thickness, axial length of eye, number of glaucoma bottles, baseline intraocular pressure (IOP), and percentage changes in IOP ([DELTA]IOP). Results: There was a significant increase in all angle parameters at the follow-up examination compared with the baseline value (all P<0.001). After multivariate analysis, the only variable related to changes in all angle parameters was [DELTA]IOP. Conclusions: Trabeculectomy results in a significant increase in the angle width in PACG. The increase in angle parameters was significantly related to the IOP changes.
    Journal of Glaucoma 10/2014; DOI:10.1097/IJG.0000000000000153 · 2.43 Impact Factor
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    ABSTRACT: To map the corneal epithelial thickness in vivo with Fourier-domain optical coherence tomography in long-term soft contact lens (SCL) wearers.
    Optometry and vision science: official publication of the American Academy of Optometry 10/2014; DOI:10.1097/OPX.0000000000000410 · 1.53 Impact Factor
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    ABSTRACT: Gap junction intercellular communication (GJIC) plays a critical role in the maintenance of corneal endothelium homeostasis. We determined if benzalkonium chloride (BAK) alters GJIC activity in the rabbit corneal endothelium since it is commonly used as a drug preservative in ocular eyedrop preparations even though it can have cytotoxic effects.
    PLoS ONE 10/2014; 9(10):e109708. DOI:10.1371/journal.pone.0109708 · 3.53 Impact Factor
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    ABSTRACT: We recently demonstrated that SERPINA3K, a serine proteinase inhibitor, has antioxidant activity in the cornea. Here we investigated the antioxidant effects of SERPINA3K on the pterygial, which is partially caused by oxidative stress in pathogenesis. The head part of primary pterygial tissue was dissected and then cultured in keratinocyte serum-free defined medium (KSFM). The cultured pterygial epithelial cells (PECs) were treated with SERPINA3K. The cell proliferation and migration of PECs were measured and analyzed. Western blot and quantitative real-time polymerase chain reaction (PCR) assay were performed. It showed that SERPINA3K significantly suppressed the cell proliferation of PECs in a concentration-dependent manner, compared with cultured human conjunctival epithelial cells. SERPINA3K also inhibited the cell migration of PECs. Towards its underlying mechanism, SERPINA3K had antioxidant activities on the PECs by significantly inhibiting NADPH oxidase 4 (NOX4), which is an important enzyme of ROS generation, and by elevating the levels of key antioxidant factors of ROS: such as NAD(P)H dehydrogenase (quinone 1) (NQO1), NF-E2-related factor-2 (NRF2) and superoxide dismutases (SOD2). Meanwhile, SERPINA3K down-regulated the key effectors of Wnt signaling pathway: β-catenin, nonphospho-β-catenin, and low-density lipoprotein receptor-related protein 6 (LRP6). We provided novel evidence that SERPINA3K had inhibitory effects on pterygium and SERPINA3K played antioxidant role via regulating the ROS system and antioxidants.
    PLoS ONE 10/2014; 9(10):e108859. DOI:10.1371/journal.pone.0108859 · 3.53 Impact Factor
    This article is viewable in ResearchGate's enriched format
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    ABSTRACT: Abstract Purpose: To evaluate meibomian gland (MG) alterations in patients with primary chronic dacryocystitis (PCD) by in vivo confocal microscopy (IVCM), and to correlate the finding with clinical presentation. Methods: Twenty-eight eyes with the diagnosis of PCD and their contralateral unaffected eyes were studied and compared with 27 normal controls. All subjects completed an Ocular Surface Disease Index questionnaire (OSDI) and underwent slit-lamp biomicroscopy examination, tear break-up time (BUT) measurements, fluorescein staining, Schirmer test I, and an IVCM examination of the MG. IVCM parameters, including the MG acinar unit density (MGAUD), periglandular inflammatory cell density (ICD), MG acinar unit longest diameter (MGALD), and MG acinar unit shortest diameter (MGASD) and their correlation with clinical data were analyzed. Results: The mean MG expressibility scores, BUT values, and staining scores were significantly worse in eyes with PCD compared with the contralateral clinically unaffected eyes and controls (p < 0.05). A significant decrease in MGAUD was observed in PCD eyes compared with the controls and the contralateral clinically unaffected eyes. Conversely, the mean ICD and MGASD values were significantly higher in the PCD eyes. There were no significant differences in mean MGALD value between the PCD eyes and the contralateral clinically unaffected eyes. In addition, there were significant changes in the IVCM parameters in the contralateral unaffected eyes compared with the controls, including MGAUD, ICD, MGALD, and MGASD. All IVCM parameters showed a strong, significant correlation with MG dropout grades, MG expressibility, fluorescein staining scores, and OSDI values (all p < 0.05). Conclusions: Patients with unilateral PCD demonstrated significant changes in MG as compared with the contralateral clinically unaffected eyes and controls. The MG function should be closely observed in these patients.
    Current Eye Research 09/2014; DOI:10.3109/02713683.2014.959608 · 1.66 Impact Factor
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    ABSTRACT: Increased tear osmolarity is one of the core mechanisms of dry eye and has been considered as an important diagnostic criterion, if not"gold standard", of dry eye. However, recent studies showed the limitations of tear osmolarity measurement not only in the diagnosis, but also in the therapeutic efficacy evaluation of dry eye. The clinical significance of tear osmolarity measurement has become a hot topic of argument. Herein, we review the publications on this topic and try to find the underlying causes of such argument.
    [Zhonghua yan ke za zhi] Chinese journal of ophthalmology 09/2014; 50(9):650-7.
  • Zuguo Liu, Jiaxu Hong
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    ABSTRACT: Translational medicine emphasizes the application of the results of basic research to clinical practice and the solution of clinical issues through basic research. It is very important for the development direction of the medical field. Over the past decade, the translational medicine research in ocular surface disease has drawn international attention and developed rapidly. This field in China has made great progress, but overall there are still some gaps with the international advanced level. We need to establish the concept and the platform of translational research, explore scientific and effective methods, and create a whole chain to enhance our ocular research. It also requires research institutions and government departments to give full support. Finally, we need to explore a suitable way for the development of Chinese translational medical research.
    [Zhonghua yan ke za zhi] Chinese journal of ophthalmology 09/2014; 50(9):646-9.
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    ABSTRACT: Dry eye is a multifactorial disease of the tears and the ocular surface. This study aimed to investigate the clinical efficacy of a non-steroidal anti-inflammatory drug, pranoprofen, in the treatment of dry eye.
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    ABSTRACT: A 56-year-old woman with a history of disposable soft contact lens wear was referred to our university eye center for a corneal ulcer. Based on the microbial culture, the initial diagnosis was bacterial keratitis, which was unresponsive to topical fortified antibiotics. The patient was then examined using in vivo confocal microscopy, which revealed Acanthamoeba infection. This case emphasizes the need to suspect Acanthamoeba infection in soft contact lens wearers who present with progressive ulcerative keratitis or progressively worsening corneal ulcers that are not responsive to the usual antimicrobial therapy. It is also important to consider the possibility of a coinfection with bacterial and Acanthamoeba species.Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/5168343391150859.
    Diagnostic Pathology 06/2014; 9(1):105. DOI:10.1186/1746-1596-9-105 · 2.41 Impact Factor
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    ABSTRACT: Benzalkonium chloride (BAC) is the most commonly used preservative in ophthalmic preparations.So far large bodies of clinical and experimental studies have shown that use of topical drugs containing BAC can induce a series of ocular surface diseases, such as apoptosis.However, recently, some clinical studies have shown that ocular toxicity in patients treated with eye drops containing BAC has not apparent correlated with BAC.Some scholars consider that the limitations of the research lead people to recognize the BAC toxicity exaggeratedly.Here we summarize numerous clinical and experimental studies of BAC in the past few years, and focus on reviewing recent researches of the toxic effect of BAC on ocular surface.
    [Zhonghua yan ke za zhi] Chinese journal of ophthalmology 04/2014; 50(4):303-6.
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    ABSTRACT: Prostaglandin (PG) analogs, including latanoprost, travoprost, and bimatoprost, are currently the most commonly used topical ocular hypotensive medications. The purpose of this study was to investigate the corneal alterations in rabbits following exposure to commercial solution of latanoprost, travoprost and bimatoprost. A total of 64 New Zealand albino rabbits were used and four groups of treatments were constituted. Commercial latanoprost, travoprost, bimatoprost or 0.02% benzalkonium chloride (BAK) was applied once daily to one eye each of rabbits for 30 days. The contralateral untreated eyes used as controls. Schirmer test, tear break-up time (BUT), rose Bengal and fluorescein staining were performed on days 5, 10, 20, and 30. Central corneal changes were analyzed by in vivo confocal microscopy, and the corneal barrier function was evaluated by measurement of corneal transepithelial electrical resistance on day 5. Whole mount corneas were analyzed by using fluorescence confocal microscopy for the presence of tight-junction (ZO-1, occludin) and adherens-junction (E-cadherin, β-catenin) proteins, actin cytoskeleton, proliferative marker Ki67 and cell apoptosis in the epithelium. Topical application of commercial PG analogs resulted in significant corneal epithelial and stromal defects while no significant changes in aqueous tear production, BUT, rose bengal and fluorescein staining scores on day 5. Commercial PG analogs induced dislocation of ZO-1 and occludin from their normal locus, disorganization of cortical actin cytoskeleton at the superficial layer, and disruption of epithelial barrier function. The eyes treated with 0.02% BAK and latanoprost exhibited significantly reduced Schirmer scores, BUT, and increased fluorescein staining scores on days 10 and 30, respectively. Topical application of commercial PG analogs can quickly impair the corneal epithelium and stroma without tear deficiency. Commercial PG analogs break down the barrier integrity of corneal epithelium, concomitant with the disruption of cell junction and actin cytoskeleton between superficial cells in the corneal epithelium in vivo.
    PLoS ONE 03/2014; 9(3):e89205. DOI:10.1371/journal.pone.0089205 · 3.53 Impact Factor
    This article is viewable in ResearchGate's enriched format
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    ABSTRACT: There are several animal models illustrating dry eye pathophysiology. Current study would like to establish an ex vivo tissue culture model for characterizing dry eye. Human conjunctival explants were cultured under airlift or submerged conditions for up to 2 weeks, and only airlifted conjunctival cultures underwent increased epithelial stratification. Starting on day 4, the suprabasal cells displayed decreased K19 expression whereas K10 keratin became evident in airlift group. Pax6 nuclear expression attenuated already at 2 days, while its perinuclear and cytoplasmic expression gradually increased. MUC5AC and MUC19 expression dramatically decreased whereas the full thickness MUC4 and MUC16 expression pattern disappeared soon after initiating the airlift condition. Real time PCR showed K16, K10 and MUC16 gene up-regulated while K19, MUC5AC, MUC19 and MUC4 down-regulated on day 8 and day 14. On day 2 was the appearance of apoptotic epithelial and stromal cells appeared. The Wnt signaling pathway was transiently activated from day 2 to day 10. The inflammatory mediators IL-1β, TNF-α, and MMP-9 were detected in the conditioned media after 6 to 8 days. In conclusion, airlifted conjunctival tissue cultures demonstrated Wnt signaling pathway activation, coupled with squamous metaplasia, mucin pattern alteration, apoptosis and upregulation of proinflammatory cytokine expression. These changes mimic the pathohistological alterations described in dry eye. This correspondence suggests that insight into the pathophysiology of dry eye may be aided through the use of airlifted conjunctival tissue cultures.
    PLoS ONE 01/2014; 9(1):e87368. DOI:10.1371/journal.pone.0087368 · 3.53 Impact Factor
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    ABSTRACT: To determine the vision-related quality of life (VR-QOL) in patients with infectious keratitis using the 25-item National Eye Institute Visual Function Questionnaire (NEI VFQ-25). Sixty-five patients with infectious keratitis (IK) were enrolled in the study. The NEI VFQ-25 scores and clinical and demographic data, including age, gender, pathogen, best corrected visual acuity (BCVA), and duration of the disease, were collected from the subjects. The subscale and composite scores were calculated and analyzed. Correlations between the VFQ-25 scores and the clinical and demographic features were also explored. The mean age of enrolled subjects was 48.4 years (SD, 16.2), with 44 males (67.7%). The microbial pathogens were viruses (n = 48, 73.8%), fungi (n = 13, 20.0%), and bacteria (n = 4, 6.2%). The mean scores of each VFQ-25 subscale ranged from 31.9 (SD, 28.6) for role difficulties to 92.7 (SD, 13.1) for color vision; the mean composite score was 58.1 (SD, 19.2). Significant differences in scores were observed only in the subscale of dependency among educational levels and in the mental health subscale and the composite among the three pathogen groups. Multivariate regression analysis revealed that VFQ-25 composite score correlated significantly with the BCVA of the worse-seeing eye, duration of the disease, history of operation (for IK treatment), and gender. Infectious keratitis has extensive impacts on patients and VR-QOL. The BCVA of worse-seeing eye, duration, history of operation for IK treatment, and gender contributed independently to VR-QOL. Early treatment should be encouraged to obtain better visual prognosis and VR-QOL for patients with IK.
    Optometry and vision science: official publication of the American Academy of Optometry 01/2014; DOI:10.1097/OPX.0000000000000156 · 1.53 Impact Factor
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    ABSTRACT: To investigate the effects and mechanism of amniotic extraction on corneal healing after photorefractive keratectomy (PRK). Experimental Study. Thirty-six New Zealand rabbit corneas were performed with PRK models (-10 diopters, 6.5 mm diameter). According to random number table, all eyes were divided into three groups, including treated with amniotic extraction, 0.1% dexamethasone and excipient respectively after operation. Clinical and histopathologic examinations were taken by slit-lamp microscope and light microscope. Corneal epithelium reparation was observed by fluorescent staining. Corneal stroma cell apoptosis was evaluated by terminal deoxyribonucleotidy transferase-mediated deoxynuridine triphophate nick end labeling (TUNEL) assay. Myofibroblast generation was evaluated by immunofluorescence checking the expression of alpha-smooth muscle actin (α-SMA). The number of TUNEL and α-SMA positive cells was analyzed to explore the effects on corneal haze. The haze grading was compared between groups using Kruskal-Wallis H test. Mean values for each experiment were compared between groups using a one-way analysis of variance and LSD-t test.Spearman rank analysis was used to evaluate the correlation between the haze grading and the expression of TUNEL positive cells and α-SMA. The corneas of seventy-two eyes reepithelialized in 6 days after operation. The average epithelium repair time of the AE group was (4.12 ± 0.62) d, the dexamethasone group was (5.25 ± 0.78) d, and the excipient group was (4.96 ± 0.73) d. The progression of reepithelialization was significantly faster in the AE group than the other two groups (F = 14.144, P < 0.01). The haze appeared in the first week after the PRK in all three groups, increased after 3-4 weeks, and relieved after 8 weeks. The degree of haze was significantly lower in the AE group than the other two groups in the first week (Vs. dexamethasone group, H = 3.995, P < 0.05; vs. excipient group, H = 12.77, P < 0.01), in the 4th week (Vs. dexamethasone group, H = 4.468, P < 0.05;vs. excipient group, H = 9.003, P < 0.01), and 8th week (Vs dexamethasone group, H = 4.397, P < 0.05;vs. excipient group, H = 5.744, P < 0.05) after PRK. The TUNEL-positive cells appeared in the central anterior stroma at the first week after surgery. And the number of TUNEL-positive cells in the AE group was (2.2500 ± 0.3750) cells/HP, the dexamethasone group was (4.5000 ± 0.7500) cells/HP, and the excipient group was (7.1250 ± 0.9063) cells/HP. The number of TUNEL-positive cells in AE group was less than those in the other two groups (Vs. dexamethasone group, t = 4.26, P < 0.01; vs. excipient group, t = 8.13, P < 0.01). The TUNEL-positive cells were only found in the excipient group (2.8750 ± 0.6563)/HP in 4th week after operation.It was significantly different between the dexamethasone group and the excipient group (t = 9.01, P < 0.01). There were no significant TUNEL-positive cells in 8th weeks in all three groups.α-SMA-positive cells started to appear apparently at the first week after surgery in the dexamethasone and excipient groups, and the peaks appeared at the 4th week after treatments, and there were still a lot of α-SMA-positive cells in corneal stroma at the 8th week after operation in both groups.On the contrary, there were no significant α-SMA-positive cells in the AE group all the time after surgery. The statistical significant difference can be found between the AE group vs. the dexamethasone and excipient groups in the first week (t = 28.62, 36.55;P < 0.01), in the 4th week (t = 30.40, 35.96; P < 0.01), and in the 8th week (t = 34.02, 38.32; P < 0.01).Spearman rank analysis demonstrated that the formation of haze was proportional to the expression of TUNEL positive cells (r = 0.881, P < 0.01) and α-SMA (r = 0.710, P < 0.01). Amniotic extraction can reduce the formation of haze, which was more effective than 0.1% dexamethasone.It might release certain factors which were transported into corneal matrix, then affected the healing of epithelial cell by interacting with the corneal cell factors, reducing the cell apoptosis, corneal wound healing response and rebuilding the corneal matrix with less myofibroblast, collagen and scar and finally reduce the formation of haze.
    [Zhonghua yan ke za zhi] Chinese journal of ophthalmology 01/2014; 50(1):42-50.
  • Ophthalmology 12/2013; 120(12):e86. DOI:10.1016/j.ophtha.2013.08.019 · 5.56 Impact Factor
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    ABSTRACT: Twist2 has been shown to promote human tumor invasion as in breast cancer and cervical cancer. However, whether Twist2 promotes human ovarian cancer progression remains to be elucidated. Here, we investigate the role of Twist2 in ovarian cancer invasion and metastasis as well as the underlying molecular mechanisms. Twist2 expression was detected by Immunohistochemistry (IHC) on tissue microarray of human ovarian cancers with scoring procedure according to the staining intensity and pattern. Twist2 gene was stably introduced into SKOV-3 ovarian cancer cells to examine the changes of cellular morphology, motility, invasiveness, and EMT molecular markers. Twist2 expression is significantly increased in ovarian cancers along with the FIGO disease stage, indicating that Twist2 may be associated with ovarian cancer metastasis. Overexpression of Twist2 induced the EMT phenotype including downregulation of E-cadherin, and upregulation of N-cadherin and β-catenin in human ovarian cancer cells, suggesting that Twist2 might promote β-catenin release from the E-cadherin/β-catenin complex through inhibition of E-cadherin. Thus, β-catenin degradation was inhibited due to inhibition of APC, and the Wnt/β-catenin pathway was then activated by nuclear β-catenin accumulation, which may activate transcription of downstream target genes to promote tumor invasion and metastasis. Collectively, these data indicated that β-catenin is involved in Twist2-induced EMT in ovarian cancer. Our data indicates that upregulation of Twist2 is correlated with the FIGO stage in human ovarian cancers. In this report, we demonstrated that nuclear β-catenin is accumulated in Twist2-induced EMT cells to facilitates ovarian cancer invasion and metastasis.
    PLoS ONE 11/2013; 8(11):e78200. DOI:10.1371/journal.pone.0078200 · 3.53 Impact Factor
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    ABSTRACT: To establish normal noninvasive tear film breakup time (NI-BUT) values in the Chinese population and investigate age-related changes in NI-BUT using a newly developed Keratograph. Forty normal volunteers with a mean age of 32.8 ± 16.7 years were recruited for this study. Clinical and demographic data, including age, gender, fluorescein tear film breakup time (FBUT), and Schirmer I test values were collected from the subjects. Noninvasive tear film breakup time was measured using a new method based on a corneal topographer equipped with a modified scan software. The correlations between the NI-BUT, age, and gender were determined. In total, a significant difference between the NI-BUT and the FBUT was found (4.9 ± 2.4 seconds vs. 9.0 ± 3.0 seconds; p < 0.001). No statistically significant difference in the NI-BUT was observed between the male and female subjects (5.5 ± 2.0 seconds vs. 4.5 ± 2.5 seconds; p = 0.137). In addition, no significant correlation was detected between the NI-BUT and age (0.143, p = 0.321). The NI-BUT values found in this study are much lower than those of previous reports. Our results show no significant differences in tear film stability with age. The tear physiology of the Chinese population may not be the same as in Western populations.
    Optometry and vision science: official publication of the American Academy of Optometry 11/2013; DOI:10.1097/OPX.0000000000000126 · 1.53 Impact Factor

Publication Stats

418 Citations
187.67 Total Impact Points

Institutions

  • 2008–2014
    • Xiamen University
      Amoy, Fujian, China
  • 2013
    • Fudan University
      • Department of Ophthalmology
      Shanghai, Shanghai Shi, China
  • 2006–2009
    • Sun Yat-Sen University
      • State Key Laboratory of Oncology
      Shengcheng, Guangdong, China
  • 2002–2005
    • Zhongshan University
      中山, Guangdong, China
    • Sun Yat-Sen University of Medical Sciences
      Shengcheng, Guangdong, China