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Zheng Zhang,
Xiaoyan Lv,
Yidong Wang, Ye Chen,
Rong Zheng,
Huan Sun,
Guohui Bian,
Yan Xiao,
Qingwei Li,
Qiutan Yang,
Jianzhong Ai,
Jingjing Duan,
Ruizhi Tan,
Yuhang Liu,
Yang Yang,
Yuquan Wei,
Qin Zhou
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ABSTRACT: Embryos transfer is one of the most critical steps for generating genetically modified mice. Because of the limitations of the current transfer equipment and techniques, such as discharge of freshly transferred embryos and the necessity of sophisticated transfer skills, the success ratio of implantation may not well satisfy the requirements for mass production of high quality animals in the field of genetically modified mice. In this study, we describe a modified embryo transfer pipette with a syringe-like tip that can easily be applied to mouse embryo transfer. Using this improved method, we show that the procedures for mouse embryo implantation are simplified and easier to perform; moreover, the viability rate of mouse embryos is 20% higher than that achieved with conventional methods. Our modified tool and improved transfer technique are effective, time-saving and less invasive, resulting in increased success of embryo transfer.
Journal of Reproduction and Development 12/2008; 55(1):94-7. · 1.46 Impact Factor
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Yi-Dong Wang,
Guo-Hui Bian,
Xiao-Yan Lv,
Rong Zheng,
Huan Sun,
Zheng Zhang, Ye Chen,
Qin-Wei Li,
Yan Xiao,
Qiu-Tan Yang,
Jian-Zhong Ai,
Yu-Quan Wei,
Qin Zhou
[show abstract]
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ABSTRACT: Although previous studies have implicated a role for TC1 (C8orf4) in cancer cell proliferation, the molecular mechanism of its action is still largely unclear. In this study, we showed, for the first time, that the mRNA levels of TC1 were upregulated by mitogens (FBS/thrombin) and at least partially, through the ERK1/2 signaling pathway. Interestingly, the over-expression of TC1 promoted the G(1)- to S-phase transition of the cell cycle, which was delayed by the deficiency of ERK1/2 signaling in fibroblast cells. Furthermore, the luciferase reporter assay indicated that the over-expression of TC1 significantly increased Cyclin D1 promoter-driven luciferase activity. Taken together, our findings revealed that TC1 was involved in the mitogen-activated ERK1/2 signaling pathway and positively regulated G(1)- to S-phase transition of the cell cycle. Our results may provide a novel mechanism of the role of TC1 in the regulation of cell proliferation.
BMB reports 11/2008; 41(10):733-8. · 1.72 Impact Factor
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Ye Chen,
Zheng Zhang,
Xiao-Yan Lv,
Yi-Dong Wang,
Zhong-Guo Hu,
Huan Sun,
Rui-Zhi Tan,
Yu-Hang Liu,
Guo-Hui Bian,
Yan Xiao,
Qin-Wei Li,
Qiu-Tan Yang,
Jian-Zhong Ai,
Lu Feng,
Yang Yang,
Yu-Quan Wei,
Qin Zhou
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ABSTRACT: Pkd2l2 is a novel member of the polycystic kidney disease (PKD) gene family in mammals. Prominently expressed in testis, this gene is still poorly understood. In this study, reverse transcription polymerase chain reaction (RT-PCR) results showed a time-dependent expression pattern of Pkd2l2 in postnatal mouse testis. Immunohistochemical analysis revealed that Pkd2l2 encoded a protein, polycystin-L2, which was predominantly detectable in the plasma membrane of spermatocytes and round spermatids, as well as in the head and tail of elongating spermatids within seminiferous tubules in mouse testis tissue sections of postnatal day 14 and adult mice. A green fluorescent fusion protein of Pkd2l2 resided in the plasma membrane of HEK 293 and MDCK cells, suggesting that it functions as a plasma membrane protein. Overexpression of Pkd2l2 increased the intracellular calcium concentration of MDCK cells, as detected by flow cytometry. Collectively, these data indicated that Pkd2l2 may be involved in the mid-late stage of spermatogenesis through modulation of the intracellular calcium concentration.
Biological & Pharmaceutical Bulletin 09/2008; 31(8):1496-500. · 1.66 Impact Factor
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ABSTRACT: Studies have shown that polycystin-1, encoded by PKD1, the major ADPKD, may have a central role in regulating both apoptosis and proliferation, which could prevent the malignant transformation of affected cells. However, as a putative tumor suppressor, direct studies on the possibility that polycystin-1 may play a role in cancer cells' biological properties have not yet been reported. We have demonstrated that the apoptosis of cancer cells was induced by overexpression of polycystin-1. After transfection with polycystin-1, three cancer cell lines, HepG2, A549, and SW480, showed significantly increased apoptosis compared with the respective control groups. This was accompanied by cell cycle arrest at G(0)/G(1) phase, whereas cell proliferation was not significantly affected. Overexpression of polycystin-1 induces apoptosis in cancer cells, at least partially, through Wnt and a caspase-dependent pathway.
Cell Biology International 05/2008; 32(4):427-35. · 1.48 Impact Factor
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Zhong-Guo Hu, Ye Chen,
Qin Zhou,
Xiao-Yan Lv,
Zheng Zhang,
Yi-Dong Wang,
Yan Xiao,
Hong Guo,
Yu-Hang Liu,
Rui-Zhi Tan,
Qin-Wei Li,
Guo-Hui Bian,
Yu-Quan Wei
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ABSTRACT: SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.
Biochemical and Biophysical Research Communications 02/2008; 365(2):214-20. · 2.48 Impact Factor
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Ke Zhang,
Chun Ye,
Qin Zhou,
Rong Zheng,
Xiaoyan Lv, Ye Chen,
Zhongguo Hu,
Hong Guo,
Zheng Zhang,
Yidong Wang,
Ruizhi Tan,
Yuhang Liu
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ABSTRACT: The approximately 14 kb mRNA of the polycystic kidney disease gene PKD1 encodes a large ( approximately 460 kDa) protein, termed polycystin-1 (PC-1), that is responsible for autosomal dominant polycystic kidney disease (ADPKD). The unique organization of its multiple adhesive domains (16 Ig-like domains/PKD domains) suggests that it may play an important role in cell-cell/cell-matrix interactions. Here we demonstrated that PKD1 promoted cell-cell and cell-matrix interactions in cancer cells, indicating that PC-1 is involved in the cell adhesion process. Furthermore in this study, we showed that PKD1 inhibited cancer cells migration and invasion. And we also showed that PC-1 regulated these processes in a process that may be at least partially through the Wnt pathway. Collectively, our data suggest that PKD1 may act as a novel member of the tumor suppressor family of genes.
Cell Biochemistry and Function 04/2007; 25(6):767-74. · 1.77 Impact Factor
