C J Lopez-Bote

Complutense University of Madrid, Madrid, Madrid, Spain

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Publications (100)149.94 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Maternal malnutrition during pregnancy, both by deficiency and excess, induces changes in the intrauterine environment and the metabolic status of the offspring, playing a key role in the growth, status of fitness/obesity and appearance of metabolic disorders during postnatal life. There is increasing evidence that these effects may not be only limited to the first generation of descendants, the offspring directly exposed to metabolic challenges, but to subsequent generations. The present study evaluated, in a swine model of obesity/leptin resistance, the existence and extent of transgenerational developmental programming effects. Pre- and postnatal development, adiposity and metabolic features were assessed in the second generation of piglets descendant of sows exposed to either under- or overnutrition during pregnancy. The results indicated that these piglets showed early-postnatal increases in adiposity and disturbances in lipids profiles compatible with the early prodrome of metabolic syndrome, with liver tissue also evidencing pediatric liver disease. These features evidencing early-life metabolic disorders were more evident in the males that were descendant from overfed grandmothers and during the transition from milk to solid feeding. Thus, the present study gives evidence supporting transgenerational developmental programming and supports the necessity of the development of strategies for avoiding the current epidemics of childhood overweight and obesity.
    The Journal of endocrinology. 08/2014;
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    ABSTRACT: The two main genetic types in Iberian pig production show important phenotypic differences in growth, fattening and tissue composition since early developmental stages. The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. Contemporary families coming from pure Iberian pigs (IB) or from crossing with Duroc boars (DUxIB) were generated. Piglets (14 from each genetic type) were slaughtered at weaning (28 days) and longissimus dorsi was sampled for composition and gene expression studies. RNA was obtained and hybridized to Affymetrix Porcine Genechip expression arrays.
    BMC genomics. 05/2014; 15(1):413.
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    ABSTRACT: To determine the feasibility of MRI for imaging subcutaneous and visceral adiposity in longitudinal studies in obese swine models (Iberian pig). To establish the anatomical regions of interest (ROIs) and measurement points (MPs) adequate for their evaluation through analyses on the inter-individual variability and over-time reproducibility and through the assessment of their reliability and validity by comparison with in vivo and ex vivo zoometric data. Five male and five female pigs were used from four (live weight around 48kg and back-fat depth around 18mm) to eight months old (live weight 134kg and back-fat depth around 48mm). MRI was carried out with a Panorama 0.23T scanner (Philips Medical Systems, Best, Netherlands), using a body/spine XL coil. The ROIs of election for visualization of subcutaneous data are located from the cranial margin of left diaphragmatic crura to the lumbar vertebrae L3. Visceral adiposity may be equally evaluated from the vertebrae L1 to L3. MRI allows the evaluation of subcutaneous and visceral fatness in a single acquisition, which improves animal welfare and time- and cost-efficiency and provides an accurate, consistent and repeatable procedure for sequential studies of adiposity in obese swine.
    Diagnostic and interventional imaging. 04/2014;
  • D Amazan, G Cordero, C J López-Bote, C Lauridsen, A I Rey
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    ABSTRACT: This study evaluated the strategy of supplementing oral micellized natural vitamin E (d-α-tocopherol) to either piglets and/or sows on α-tocopherol concentrations in piglets serum and tissues after weaning. One first experiment tested the influence of the vitamin E supplementation source (natural form in water v. the synthetic form in feed) and dose administered to piglets and/or sows on serum α-tocopherol concentration, α-tocopherol stereoisomer accumulation, antioxidant capacity and immune response of weaned piglets. A second experiment studied the effect of sow source and dose vitamin E supplementation on some of these parameters in piglets. Oral supplementation to sows with natural vitamin E as a micellized form (d-α-tocopherol) at the lowest dose produced a similar concentration of α-tocopherol in serum at days 2, 14 and 28 postpartum to those supplemented with threefold higher dose of the synthetic form in feed. At day 39 of age, neither piglet supplementation source nor dose significantly affected α-tocopherol accumulation in the serum, muscle, subcutaneous fat or liver. Those piglets from sows supplemented with the micellized alcohol form had higher RRR-α-tocopherol stereoisomers (P<0.001) and lower (P<0.001) RRS- RSS- and RSR-α-tocopherol, at day 39 of age than those from sows supplemented with the synthetic form. A predominant importance of sow over piglet vitamin E supplementation was observed on stereoisomer distribution in piglets. Low doses of oral natural vitamin E supplementation to sows or piglets did not increase the oxidative stress of piglets when compared with the use of the synthetic form in feed. Immunoglobulin levels in piglet serum at day 39 were not affected by natural vitamin E supplementation at low doses in drinking water of piglets or sows when compared with the synthetic form in feed. IgA tended to be higher (P=0.145) at day 39 in piglets supplemented with natural vitamin E when compared with those supplemented with the synthetic form. Low doses of oral micellized natural vitamin E supplementation to sows is an interesting feeding strategy, when compared with the use of high doses of the synthetic form in feed, because it results in similar α-tocopherol concentrations, allows a predominant -R stereoisomer distribution in piglets and also maintains their oxidative status in vivo.
    animal 03/2014; 8(3):410-9. · 1.65 Impact Factor
  • J Segura, C J Lopez-Bote
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    ABSTRACT: A new procedure to extract intramuscular fat (IMF) was developed to minimize sample amount, solvent use and time of analysis. Lyophilised samples (200mg) were accurately weighed in a safe-lock micro test tube, homogenized in 1.5mL dichloromethane-methanol (8:2) and mixed in a mixer mill (MM400, Retsch technology). The final biphasic system was separated by centrifugation (8min, 10,000rpm). The extraction was repeated three times. Solvent was evaporated under nitrogen stream and lipid content was gravimetrically determined. Results from 64 determinations were compared to those obtained with other referred method and showed a linear response over the whole range of IMF content (1.6-6.9g/100g sample). Moreover, the analysis with different methodology of six replica from the same sample showed lowest variability (standard deviation intra-method) for the new methodology proposed over a wide range of IMF content. A cost and time efficient lipid extraction procedure was developed without loss of precision and accuracy and with a fatty acid profile comparable to other protocols.
    Food Chemistry 02/2014; 145C:821-825. · 3.33 Impact Factor
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    ABSTRACT: A trial was carried out to compare the effects of different dietary sources of monounsaturated fatty acids (MUFA), on the fatty acid (FA) composition, lipid metabolism and gene transcription in different tissues of Iberian pigs. Twenty-seven Iberian male pigs of 28 kg Live Weight (LW) were divided in two groups and fed with one of two isocaloric diets: a standard diet with carbohydrates as energy source (CH) and a diet enriched with high oleic sunflower oil (HO). Ham adipose tissue was sampled by biopsy at 44 and 70 kg LW. At 110 kg LW pigs were slaughtered and backfat, loin and liver tissues were sampled. Animals of the HO group showed higher MUFA content and lower saturated fatty acids (SFA) in all the analysed tissues (P<0.001). These main effects were established early during the treatment and increased only slightly along time. Small diet effects were also detected on polyunsaturated fatty acids (PUFA), which showed differences according to sampling time, tissue and lipid fraction. Effects of diet on gene expression were explored with a combined approach analyzing adipose tissue transcriptome and quantifying the expression of a panel of key genes implicated in lipogenesis and lipid metabolism processes, in backfat, muscle and liver. Backfat transcriptome showed small effects of diet on gene expression, in number and magnitude. According to the posterior probabilities (PP) of the probe-specific expression differences between dietary groups (PP<0.01), 37 genes were considered differentially expressed (DE). Gene ontology allowed relating them with several biological functions including lipid metabolic processes. Quantitative PCR confirmed several DE genes in adipose tissue (RXRG, LEP and ME1, P<0.0001, P<0.05 and P<0.0001, respectively), but no DE gene was found in loin or liver tissues. Joint results agree with a metabolic adjustment of adipose tissue fatty acids levels by the subtle effect of the diet on the regulation of several lipid metabolism pathways, mainly FA oxidation and prostanoid synthesis, with LEP, RXRG and PTGS2 genes playing mayor roles.
    Journal of Animal Science 02/2014; · 2.09 Impact Factor
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    ABSTRACT: Maternal energy restriction during pregnancy predisposes to metabolic alterations in the offspring. The present study was designed to evaluate phenotypic and metabolic consequences following maternal undernutrition in an obese pig model and to define the potential role of hypothalamic gene expression in programming effects. Iberian sows were fed a control or a 50 % restricted diet for the last two-thirds of gestation. Newborns were assessed for body and organ weights, hormonal and metabolic status, and hypothalamic expression of genes implicated in energy homeostasis, glucocorticoid function and methylation. Weight and adiposity were measured in adult littermates. Newborns of the restricted sows were lighter (P <0·01), but brain growth was spared. The plasma concentration of TAG was lower in the restricted newborns than in the control newborns of both the sexes (P <0·01), while the concentration of cortisol was higher in females born to the restricted sows (P <0·04), reflecting a situation of metabolic stress by nutrient insufficiency. A lower hypothalamic expression of anorexigenic peptides (LEPR and POMC, P <0·01 and P <0·04, respectively) was observed in females born to the restricted sows, but no effect was observed in the males. The expression of HSD11B1 gene was down-regulated in the restricted animals (P <0·05), suggesting an adaptive mechanism for reducing the harmful effects of elevated concentrations of cortisol. At 4 and 7 months of age, the restricted females were heavier and fatter than the controls (P< 0·01). Maternal feed restriction induces asymmetrical growth retardation and metabolic alterations in the offspring. Differences in gene expression at birth and higher growth and adiposity in adulthood suggest a female-specific programming effect for a positive energy balance, possibly due to overexposure to endogenous stress-induced glucocorticoids.
    The British journal of nutrition 02/2014; 111(4):735-46. · 3.45 Impact Factor
  • A Daza, M A Latorre, A Olivares, C J López-Bote
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    ABSTRACT: A total of 48 Duroc×(Landrace×Large White) gilts of 33.2 kg BW were used to investigate the influence of immunocastration and diet on growth performance and carcass, meat and fat quality. Four treatments were arranged factorially (2×2) with two sexes (immunocastrated gilts: IG v. entire gilts: EG) and two dietary treatments (a commercial feedstuff as control v. granulated barley as a single major ingredient) provided during the finishing period (from 103 to 126 kg BW). There were four replicates of three pigs per treatment. At the end of the trial, the IGs grew faster (P<0.05) and ate more feed (P<0.05) than the EGs. Carcasses from the IGs had thicker backfat depth than those from the EGs (P<0.01) and carried out a lower percentage of rejected carcasses (P<0.05) at slaughterhouse owing to lack of fat. Meat from the IGs tended to have higher intramuscular fat (IMF) content in the Longissimus thoracis (LT) muscle than that from the EGs (P=0.09). In addition, immunocastration increased the total saturated fatty-acid proportion in subcutaneous fat and IMF (P<0.001) and decreased the total monounsaturated fatty acid (MUFA) and total polyunsaturated fatty acid (PUFA) percentages in subcutaneous backfat (P<0.05 and P<0.001, respectively) and in IMF (P<0.01 and P=0.06, respectively). The use of a diet based on granulated barley during the finishing period had no effect on growth performance but tended to increase IMF content in the LT muscle (P<0.06), and increased MUFA (P<0.05) and decreased PUFA (P<0.01) proportions in omental fat. It is concluded that immunocastration of gilts intended for dry-cured ham industry improved some aspects of growth performance and carcass and meat quality, whereas granulated barley had scarce effect on productive traits and fatty-acid profile but tended to improve IMF content.
    animal 01/2014; · 1.65 Impact Factor
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    ABSTRACT: Objectifs Déterminer la faisabilité de l’IRM pour la réalisation d’imageries de la graisse sous-cutanée et viscérale au cours d’études longitudinales sur des modèles porcins d’obésité (porc ibérique) in vivo. Établir les régions d’intérêt (RDI) anatomiques et les points de mesure (PM) adéquats afin de les évaluer par des analyses portant sur la variabilité inter-individuelle et leur reproductibilité au cours du temps et par l’évaluation de leur fiabilité et de leur validité par rapport à des données zoométriques in vivo et ex vivo. Matériels et méthodes Cinq porcs mâles et cinq porcs femelles âgés de quatre à huit mois ont été utilisés. L’IRM a été effectuée avec un appareil Panorama de 0,23 T (Philips Medical Systems, Best, Pays-Bas), en utilisant une antenne corps/rachis XL (body/spine XL coil). Résultats Les RDI de choix pour la visualisation des données sous-cutanées sont situées entre le bord cranial du pilier gauche du diaphragme et la vertèbre lombaire L3. L’adiposité viscérale peut également être évaluée des vertèbres L1 à L3. Conclusions L’IRM permet l’évaluation de la graisse sous-cutané et viscérale lors d’une seule acquisition, ce qui améliore le bien-être animal ainsi que les rapports temps–efficacité et coût–efficacité, et permet de réaliser une procédure précise, cohérente et répétable pour des études ultérieures de l’adiposité chez des porcs obèses.
    Journal de Radiologie Diagnostique et Interventionnelle. 01/2014;
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    A I Rey, C J López-Bote
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    ABSTRACT: The use of stereoisomers of α-tocopherol to correctly classify Iberian pig fat samples according to their feeding system was investigated. Samples were obtained over two different seasons in controlled farms from the four categories of pigs described in the Industry Quality Policy (FREE-RANGE: pigs fed exclusively under free-range conditions; FREE-FEED: pigs fed free-range and supplemented with feed; FEED-OUT: pigs fed outdoors with access to grass and a mixed diet; and FEED: pigs fed exclusively a mixed diet). A higher presence of RRR-stereoisomer indicated a greater consumption of the natural form of tocopherol provided by acorns or grass, whereas a higher proportion of S forms were related to a higher mixed diet intake. Validation results showed 90% success in fat sample classification. Analysis of the RRR-stereoisomer together with γ- and α-tocopherol determination can be considered as a potent tool for distinguishing fat from pigs fed under free-range conditions or exclusively with acorns and grass from those receiving a supplemented diet at any time of their fattening phase.
    Food Chemistry 01/2014; 142:342-8. · 3.33 Impact Factor
  • J.G. Vicente, B. Isabel, G. Cordero, C.J. Lopez-Bote
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    ABSTRACT: The purpose of this study was to compare the influence of adding 35 g/kg of different sources of fat (lard vs. sunflower oil) to diets for sows on the composition of fatty acids (FA) in the tissues of the progeny at weaning and one week after weaning (28 and 35 days of age respectively). Sows (n = 20) were fed with the experimental diets from day 35 of gestation and during lactation. When lard (L) was included in the sows’ diet, increased concentrations of C18:1n-9 were found, whereas C18:2n-6 decreased in both colostrum and milk (P<0.01). Milk from sows fed L showed higher (P<0.001) C16:0 and C18:1n-7 concentration in colostrum than those that were fed sunflower oil (SFO). On the first week after weaning, time effect was observed on intramuscular fat content in the pigs (P<0.012). Type of fat of the sow diet affected (P<0.05) C14:0, C16:1n-7, C17:1, C18:0, C18:1n-9, C18:1n-7, C18:2n-6, C20:1n-9 and C20:3n-9 concentration in the subcutaneous fat of the offspring. The intramuscular concentration of monounsaturated FA in pigs changed with time (P<0.001). In addition, an interaction between sow diet and sampling time in the pigs was observed; the decrease in the concentration of intramuscular FA was more pronounced in pigs from sows fed SFO than in pigs from sows fed the L. Moreover the L3HOAD (EC 1.1.135) activity was higher in pigs from sows fed SFO than in those from sows fed L (P<0.001). The administration of polyunsaturated FA to gestating and lactating sows increased FA beta-oxidation in pigs after weaning which could help the mobilization of body reserves in this critical period.
    Animal Feed Science and Technology 04/2013; 181(s 1–4):45–53. · 1.61 Impact Factor
  • A I Rey, J Segura, E Arandilla, C J López-Bote
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    ABSTRACT: This study tested the effect of micellized vitamin E (D-α-tocopherol; 1,400 IU/d) administered 12 and 1 h orally before training for 1 (ST-VitE) or 8 d (LT-VitE) compared to an un-supplemented control (CONTROL) on plasma α-tocopherol, thiobarbithuric acid-reactive substances (TBARS), total glutathione (GSHt), and trolox equivalent antioxidant capacity (TEAC) in 10 race horses. Different sampling times [immediately before training (BEF) and after intense training (END) or 8 h after recovery (+8 h)] were investigated. Plasma α-tocopherol concentration was greater in the ST-VitE group than the CONTROL group at +8 h (P < 0.05). Natural vitamin E supplementation increased plasma α-tocopherol (P < 0.001) in the LT-VitE group by approximately 1.6-fold at BEF, END, and +8 h. In all groups, TBARS tended to be slightly greater (P = 0.087) immediately after training when compared with values BEF or + 8 h and the lowest TBARS values were observed at +8 h in LT-VitE. Vitamin E supplementation did not affect the GSHt concentrations at BEF, END, or +8 h. The TEAC values were modified by the vitamin E administration (P = 0.010). The greatest TEAC was found in the LT-VitE group at all sampling times and similar concentrations were reached in the ST-VitE group at +8 h. The CONTROL group was not able to maintain TEAC after training (P < 0.001), indicating that consumption of antioxidants (mainly vitamin E) and consequently oxidative stress because of the antioxidant system being overwhelmed by a reduced antioxidant supply. In conclusion, micellized natural vitamin E at 1,400 IU/d for 8 d efficiently increased plasma α-tocopherol concentration of race horses undergoing intense training conditions and maintained the general oxidative status.
    Journal of Animal Science 01/2013; · 2.09 Impact Factor
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    ABSTRACT: The present study aimed to determine, in a swine model of leptin resistance, the effects of type and timing of maternal malnutrition on growth patterns, adiposity and metabolic features of the progeny when exposed to an obesogenic diet during their juvenile development and possible concomitant effects of the offspring sex. Thus, four groups were considered. A CONTROL group involved pigs born from sows fed with a diet fulfilling their daily maintenance requirements for pregnancy. The treated groups involved the progeny of females fed with the same diet but fulfilling either 160% or 50% of pregnancy requirements during the entire gestation (OVERFED and UNDERFED, respectively) or 100% of requirements until Day 35 of pregnancy and 50% of such amount from Day 36 onwards (LATE-UNDERFED). OVERFED and UNDERFED offspring were more prone to higher corpulence and fat deposition from early postnatal stages, during breast-feeding; adiposity increased significantly when exposed to obesogenic diets, especially in females. The effects of sex were even more remarkable in LATE-UNDERFED offspring, which had similar corpulence to CONTROL piglets; however, females showed a clear predisposition to obesity. Furthermore, the three groups of pigs with maternal malnutrition showed evidences of metabolic syndrome and, in the case of individuals born from OVERFED sows, even of insulin resistance and the prodrome of type-2 diabetes. These findings support the main role of early nutritional programming in the current rise of obesity and associated diseases in ethnics with leptin resistance.
    PLoS ONE 01/2013; 8(10):e78424. · 3.73 Impact Factor
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    ABSTRACT: The objective of this study was to determine, in a female swine model of leptin resistance (Iberian pig), the effect of an obesogenic diet, with high saturated fat content, during the juvenile period, on the appearance of early obesity and its possible effects on metabolic syndrome-related parameters and reproductive features (puberty attainment). Thus, from 130 to 245 days-old, seven Iberian gilts had ad libitum access to food enriched with saturated fat whilst six females acted as controls and had ad libitum access to a commercial maintenance diet. Results showed that a high fat intake-level during the juvenile period induces early obesity with lower body weight and size but a higher body fat-content. Such obesity was related with impairments of glucose regulation predisposing for insulin resistance, but also with an earlier onset of puberty. However, there were no signs of hypertriglyceridemia and hypertension; the gilts diminish their intake level and modify their metabolic features by increasing insulin secretion. In conclusion, Iberian gilts freely eating saturated fat diets during the juvenile period have the prodrome of metabolic syndrome but, during their juvenile period, are still able to develop an adaptive response to the diet.
    Endocrine, metabolic & immune disorders drug targets. 10/2012;
  • R Barea, B Isabel, R Nieto, C López-Bote, J F Aguilera
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    ABSTRACT: The lipid content and fatty acid (FA) profile in pig tissues are strongly influenced by genotype and nutrient supply, with implications in meat quality. The de novo lipid synthesis and pattern of FA unsaturation could be an important cause of variation in the overall efficiency of energy utilization among breeds. To test the effects of pig genotype and CP supply on the evolution of back-fat tissue FA profile throughout the growing and finishing stages, 32 Iberian (IB) and Landrace × Large White (LR × LW) barrows were offered one of two diets differing in CP content (13% or 17% as fed). A pair-fed procedure (0.8 × ad libitum intake of IB pigs) was used. Subcutaneous fat samples were taken at the dorso-lumbar region at ∼38, 50, 65, 90 and 115 kg BW. Higher proportions of total monounsaturated FA (MUFA; P < 0.01) and lower proportions of total saturated FA (SFA; P < 0.01 to 0.05) were found in the outer back-fat layer of pigs both at 50 and 115 kg BW. Pig genotype affected the FA composition of both subcutaneous back-fat layers. The proportions of C18:0 and SFA in fat tissue were higher in IB than in LR × LW pigs from 38 to 65 kg BW, especially in the outer layer. In addition, MUFA contents were higher in IB pigs at 115 kg BW in both layers (+5% on average; P < 0.01). Increased proportions of C18:2 n-6 and polyunsaturated FA (PUFA) were found in LR × LW pigs, irrespective of the stage of growth and back-fat layer (P⩽0.02). At 50 kg BW, pigs receiving the high-protein diet presented the highest C18:2 n-6, C18:3 n-3, C20:5 n-3 and PUFA contents. A significant genotype × CP content interaction was observed for C18:3 n-3 because of the increased concentration of this FA in LR × LW pigs when offered the 17% CP diet (P < 0.05). Higher C16:0 and SFA contents (+5%; P = 0.03) were found in pigs offered the 13% CP diet and slaughtered at 115 kg BW. There was a genotype × CP interaction for MUFA concentration because of the higher MUFA content observed in IB pigs offered the highest protein content diet (P = 0.03). Our results suggest that genetic variation in de novo lipid synthesis and pattern of FA unsaturation might contribute to explain differences in back-fat FA profile of IB and LR × LW pigs under identical nutritional management. They could be also relevant to explain the low efficiency of nutrient and energy utilization in the IB pig.
    animal 10/2012; · 1.65 Impact Factor
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    A Daza, M A Latorre, C J López-Bote
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    ABSTRACT: A total of 48 Duroc × (Large White × Landrace) gilts of 46.8 kg BW (86 ± 3 days of age) were used to investigate the effect of diet during the growing and finishing periods on growth performance and carcass, meat and fat quality. The control diet consisted of a commercial feedstuff and the granulated barley diet had that cereal as the single major ingredient. There were three treatments: (i) control diet provided from 45.6 to 127.8 kg BW (C group), (ii) control diet from 47.0 to 91.8 kg BW and granulated barley from 91.8 to 129.7 kg BW (C + GB group) and (iii) granulated barley from 47.9 to 93.1 kg BW and control diet from 93.1 to 135.1 kg BW (GB + C group). Each treatment was replicated eight times, with two pigs per replicate. The C group grew faster (P < 0.001) and had a better feed conversion ratio (P < 0.001) than the GB + C group, with C + GB being intermediate. Carcasses from C + GB gilts had higher backfat depth than those from C gilts, with GB + C being intermediate (P < 0.05). Also, the main joints (ham + shoulder + loin) had a higher (P < 0.01) yield in carcass in the GB + C group than in the C group, with C + GB being intermediate. The intramuscular fat (IMF) content was higher (P < 0.001) in loin from C + GB and GB + C gilts than in C gilts. The IMF of loin from C + GB gilts had higher (P < 0.05) C18:1n-9 and total monounsaturated fatty acid (FA) proportions than that from C gilts, whereas the C18:2n-6 and total polyunsaturated FA percentages were lower (P < 0.05) in C + GB gilts than in the remaining gilts. The total saturated FA percentage was lower (P < 0.05) in loin from GB + C than in that from C gilts. Hams from C + GB and GB + C gilts had higher (P < 0.05) C18:1n-9 and total monounsaturated FA proportions and lower C18:2n-6 and total polyunsaturated FA contents than those from C gilts. We can conclude that granulated barley provided during the growing or the finishing period improved some carcass and meat characteristics of heavy gilts desirable for dry-cured ham production.
    animal 09/2012; 6(9):1543-53. · 1.65 Impact Factor
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    ABSTRACT: The effects of undernutrition during pregnancy on prenatal and postnatal development of the offspring were evaluated in sows with obesity/leptin resistance. Females were fed, from day 35 of pregnancy onwards, a diet fulfilling either 100% (group control, n=10) or 50% of the nutritional requirements (group underfed, n=10). In the control group, maternal body weight increased during pregnancy (P<0.05) while it decreased or remained steady in the underfed group. At days 75 and 100 of gestation, plasma triglycerides were lower but urea levels were higher in restricted than in control sows (P<0.05 for both). Assessment of the offspring indicated that the trunk diameter was always smaller in the restricted group (P<0.01 at day 50, P<0.005 at days 75 and 100 and P<0.0001 at birth) while head measurements were similar through pregnancy, although smaller in the restricted than in the control group at birth (P<0.05). Newborns from restricted sows were also lighter than offspring from control females (P<0.01) and had higher incidence of growth retardation (P<0.01). Afterwards, during lactation, early postnatal growth in restricted piglets was modulated by gender. At weaning, males from restricted sows were still lighter than their control counterparts (P<0.05), while females from control and underfed sows were similar. Thus, the current study indicates a gender-related differential effect in the growth patterns of the piglets, with females from restricted sows evidencing catch-up growth to neutralise prenatal retardation and reaching similar development than control counterparts.
    Reproduction 06/2012; 144(2):269-78. · 3.56 Impact Factor
  • D. Amazan, A.I. Rey, E. Fernández, C.J. López-Bote
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    ABSTRACT: The influence of vitamin E supplementation (d-α-tocopherol) in drinking water administered concomitantly with the synthetic form in feed to sows during the lactation period and/or piglets post-weaned on tocopherol, and its transfer from sows to milk and piglet serum, as well as its antioxidant power and immune response, were investigated. Those piglets supplemented with natural α-tocopherol in drinking water and born from sows supplemented with the same form of vitamin E (SS-SP) had the highest serum α-tocopherol concentration at five days post-weaning, whereas the lowest serum α-tocopherol concentration was found for the group fed α-tocopheryl acetate and born from sows fed the same type of vitamin E (CS-CP). Intermediate values were found in those piglets provided with α-tocopheryl acetate born from sows supplemented with natural vitamin E in drinking water (SS-CP) and those provided with natural α-tocopherol in water born from sows supplemented with α-tocopheryl acetate (CS-SP) at five days post-weaning. From weaning to day 5, piglet serum α-tocopherol concentration decreased by 65% for CS-CP and SS-CP groups and 47% for groups CS-SP and SS-SP. The CS-SP and SS-CP groups had similar α-tocopherol concentrations in serum to the SS-SP group at 20 days post-weaning. The FRAP was significantly affected by the natural vitamin E supplementation of piglets (P = 0.037) which is in accordance with the differences observed in vitamin E concentration. The GSSH level in the SS-SP group was by approximately 20% and 25% lower (P = 0.0023) with respect to the CS-CP group at 5 and 20 days post-weaning, respectively. The immunoglobulin levels in piglet serum were not significantly affected by natural vitamin E supplementation in drinking water. The treatment effects observed were mainly due to vitamin E supplementation of piglets.
    Livestock Science. 05/2012; 145(s 1–3):55–62.
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    ABSTRACT: The objective of the present study was to determine the suitability of a swine breed with leptin resistance and predisposition to obesity (the Iberian pig) as model for studies on metabolic syndrome and type 2 diabetes. Thus, six Iberian sows had ad libitum access to food enriched with saturated fat (SFAD group; food consumption was estimated to be 4.5 kg/animal/day) whilst four females acted as controls and were fed with 2 kg/animal/day of a commercial maintenance diet. After three months of differential feeding, SFAD animals developed central obesity, dyslipidemia, insulin resistance and impaired glucose tolerance, and elevated blood pressure; the five parameters associated with the metabolic syndrome. Thus, the current study characterizes the Iberian pig as a robust, amenable, and reliable translational model for studies on nutrition-associated diseases.
    The Scientific World Journal 01/2012; 2012:510149. · 1.73 Impact Factor
  • J. Fuente, D. Tejon, A. Rey, J. Thos, C. J. Lopez-Bote
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    ABSTRACT: In the present work the carcass composition and fatty acid composition of the fat deposits of young Colmenarcña and Rubia del Molar male lambs fed with natural milk and with commercial milk replacer were studied. The daily gain during the late phase of feeding (15-30 days) was higher in the animals that were reared artificially than in those fed by their mothers (310 versus 245 g/day, p < 0.001). Carcass characteristics and dissected shoulder tissues were not markedly affected by dietary treatment. Lambs receiving sheep milk had higher concentration of C15: 0, C17: 0 and total saturated fatty acids than groups receiving milk replacer (p = 0.001). A higher rumen, reticulum, omasum, abomasum and small intestine development was also found in lambs fed by their mothers compared with those fed milk replacers (p < 0.05). The results from this experiment suggest that lambs artificially reared have a lower rumen functionality and might be slaughtered at higher weights without a concomitant loss of meat quality.ZusammenfassungEinfluß des Tränksystems auf Wachstum, Körperzusammensetzung und Entwicklung des Verdauungskanals bei LämmernJe 10 neugeborene Lämmer der Rassen Colmenareña und Rubia del Molar konnten über 30 Tage entweder an ihrer Mutter saugen oder sie wurden mit einem Milchaustauscher ernährt. Die Anfangsgewichte betrugen im Mittel 3,4 kg, die Endgewichte 10,5 kg/Lamm. Im Versuchsabschnitt vom 15.–30. Tag betrugen die Tageszunahmen der an der Mutter saugenden Lämmer 245 g und die der mit Milchaustauscher ernährten Tiere 310 g (p < 0.001). Die Körperzusammensetzung war durch die Tränke nicht signifikant beeinflußt. Die mit Muttermilch ernährten Lämmer wiesen höhere Konzentrationen an C15:0, C17:0 und insgesamt an gesättigten Fettsäuren auf als bei Einsatz von Milchaustauschern. Die saugenden Lämmer wiesen ein höheres Gewicht des vollen Magen-Darmtraktes auf (1599 bzw. 1265 g). Auch die Leergewichte von Pansen, Reticulum, Omasum, Abomasum und Dünndarm waren bei den an der Mutter saugenden Lämmern signifikant höher als bei den mit Milchaustauschern ernährten Tieren. Infolge der geringen Pansenentwicklung wird geschlußfolgert, daß die mit Milchaustauschern ernährten Lämmer ohne Beeinflussung der Fleischqualität mit einem höheren Gewicht geschlachtet werden können.
    J Anim Physiol a Anim Nutr 02/2011; 78(1‐5):75 - 83. · 1.25 Impact Factor

Publication Stats

1k Citations
149.94 Total Impact Points

Institutions

  • 1997–2014
    • Complutense University of Madrid
      • • Department of Animal Production
      • • Facultad de Veterinaria
      • • Departamento de Bioquímica y Biología Molecular IV
      Madrid, Madrid, Spain
  • 2005–2012
    • Universidad Politécnica de Madrid
      • Departamento de Producción Animal
      Madrid, Madrid, Spain
  • 2010
    • Hospital Universitario Miguel Servet
      Caesaraugusta, Aragon, Spain
  • 2001
    • University College Cork
      • School of Food and Nutritional Sciences
      Cork, M, Ireland
  • 1998
    • Michigan State University
      • Department of Food Science and Human Nutrition
      East Lansing, MI, United States
  • 1988–1998
    • Universidad de Extremadura
      • Facultad de Veterinaria
      Cáceres, Extremadura, Spain