C J López-Bote

Complutense University of Madrid, Madrid, Madrid, Spain

Are you C J López-Bote?

Claim your profile

Publications (114)190.4 Total impact

  • Grasas y Aceites 06/2015; 66(2):e076. DOI:10.3989/gya.1073142 · 1.08 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Vitamin A is a key regulator of gene expression, influencing adipogenesis and lipid metabolism in animal tissues. This experiment was conducted to assess the effect of dietary vitamin A level and administration time on productive traits, intramuscular fat (IMF) content in ham muscles, tissue fatty acid composition, and expression of a panel of adipogenic and lipogenic candidate genes in Iberian pigs. Sixty piglets of 16.3 kg (SD = 2.5 kg) live weight (LW) were either fed a vitamin A-enriched diet (10,000 IU vitamin A/kg; CONTROL, = 20) or a diet without supplemented vitamin A, applied from 16.3 kg (SD = 2.5 kg; early restriction group, ER, = 20) or from an average weight of 35.8 kg (SD = 3.1 kg; late restriction group, LR, = 20). Two slaughters were performed when pigs reached the averaged weights of 101.4 (SD = 4.1 kg) and 157.9 kg LW (SD = 7 kg) and samples from liver, heart, and backfat were obtained in both sacrifice times. In addition, ham subcutaneous fat and (SM) and (BF) muscles were sampled at the last sacrifice. Dietary vitamin A level produced no effect on carcass traits in any of the harvests, while a small effect was observed on fatty acid composition in backfat at 101.4 kg LW. However, at 157.9 kg LW, the ER and LR groups showed higher MUFA content and lower SFA content in backfat, ham fat, and IMF ( < 0.01). In IMF, a decrease in n-6/n-3 PUFA ratio was observed in the restricted groups ( < 0.005). Intramuscular fat content in SM muscle was greater ( < 0.05) in the ER group than in the CONTROL and LR groups, while no difference was detected in BF muscle. Little effect of dietary vitamin A was observed in liver. Regarding changes in gene expression, , , and genes were upregulated ( < 0.0001, < 0.0001, and < 0.05, respectively) in the ER group in hepatic tissue, whereas and genes were upregulated ( < 0.05) in the same group in adipose tissue. On the other hand, was downregulated ( < 0.05) in the ER group in adipose tissue. Results found in this experiment show that long-term restriction of dietary vitamin A has a positive effect on nutritional and sensorial parameters of ham meat. Moreover, gene expression results were consistent with the vitamin A transcriptional regulation of adipogenesis and lipogenesis and with the changes observed in meat and fat composition.
    Journal of Animal Science 06/2015; DOI:10.2527/jas.2014-8573 · 1.92 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Analysis of fatty acid (FA) positional distribution within the triglyceride (TG) and selected physical properties of dry-cured ham subcutaneous fat (SF) were carried out. The slip point (SP) of the SF was related to the concentration and positional distribution of FA. When C16:0 was in Sn-2, the SP depended on the FA present in Sn-1,3. Hardness was related to the FA concentration in external positions of TG. A significant direct linear correlation between hardness against C18:0, SFA and average chain length (ACL) and inverse against C18:1, C18:2 and PUFA and unsaturation index (UI) in external positions was found. Adhesiveness was related to SFA, C16:0, C18:0, C18:1, MUFA, UI and ACL exclusively in Sn-2 position. A different role of FA in Sn-2 and Sn-1,3 positions on SP and texture properties of fat was found.
    Meat Science 05/2015; 103. DOI:10.1016/j.meatsci.2015.01.008 · 2.23 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate whether dietary vitamin A level is associated with differences in adipocyte differentiation or lipid accumulation in Iberian pigs at early growing (35.8kg live weight) and at finishing (158kg live weight). Iberian pigs of 16.3kg live weight were allocated to two feeding groups, one group received 10,000IU of vitamin A/kg diet (control); the other group received a diet with 0IU of vitamin A (var) for the whole experimental period. The dietary vitamin A level had no effect on growth performance and carcass traits. The early suppression of vitamin A increased the preadipocyte number in Longissimus thoracis (LT) muscle in the early growth period (P<0.001) and the neutral lipid content and composition (higher MUFA and lower SFA content) at the end of the finishing period (P<0.05). Vitamin A restriction in young pigs increases their lipogenic potential without affecting carcass traits. Copyright © 2015 Elsevier Ltd. All rights reserved.
    Meat Science 05/2015; 108. DOI:10.1016/j.meatsci.2015.04.017 · 2.23 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study evaluates the effect of vitamin E supplementation source (micellized natural vs. the synthetic form) and dosage (40, 80, or 120 mg/kg) on α-tocopherol concentration in plasma and muscle, antioxidant capacity, and breast meat quality in turkeys. Three hundred female turkeys were randomly selected at an average live weight 63.2 g ± 0.5 and distributed into 7 groups. One group (control) was fed a standard diet without vitamin E supplementation and the other 6 were given mixed diets supplemented with the natural (d-α-tocopherol) or synthetic (dl-α-tocopheryl acetate) form of vitamin E in 3 dosages (40, 80, or 120 mg/kg). Following 11 wk feeding, results showed that performance parameters were not modified either by source or dosage of vitamin E supplementation to the turkeys. Plasma and muscle α-tocopherol at d 9 of refrigerated storage were higher when turkeys were supplemented with the natural form at higher doses. Losses in the concentration of α-tocopherol in meat between the beginning and the end of the 9 d refrigerated storage were greater in the groups supplemented with the synthetic form of vitamin E compared to those receiving the natural supplementation. The relationship between plasma α-tocopherol and the Trolox equivalent antioxidant capacity followed a different trend depending on the vitamin E source. Intramuscular fat was not significantly affected by the vitamin E source supplementation; however the slope of the linear regression equation was lower for the natural form than for the synthetic form. Turkeys given the natural form had higher C18:1n-9 but lower C15:1, C17:1, C20:5n-3, and C22:6n-3 in breast muscle. Meat samples from turkeys supplemented with natural vitamin E had higher deoxymyoglobin at d 3, 6, and 9 and lower metmyoglobin at d 9 of refrigerated storage than those receiving the synthetic form. Dietary supplementation with medium doses (80 mg/kg) micellized d-α-tocopherol is an interesting feeding strategy for ensuring antioxidant status and improving meat quality. © 2015 Poultry Science Association Inc.
    Poultry Science 03/2015; 94(6). DOI:10.3382/ps/pev091 · 1.67 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Vitamin supplementation is a widely extended practice in swine nutrition. Certain vitamins such as vitamins A and E are related to meat quality and have been reported as antagonists. Thus, their tissue levels are of interest for swine producers and consumers. This experiment was undertaken to study the effect of dietary vitamin A supplementation or withdrawal duration and timing on the evolution of vitamin A deposition in tissues, α-tocopherol accumulation and gene expression in heavy pigs. Eighty weaned Iberian piglets (16.3 ± 2.5 kg) were either fed a vitamin A-enriched diet (10,000 IU vitamin A/kg) (CONTROL) or given a diet without added vitamin A applied from the beginning of the trial at 16.3 kg (early restriction group, ER) or from an average weight of 35.8 kg (late restriction group, LR). Pigs fed ER and LR had lower ADG and worse feed efficiency than those from the CONTROL group at 101.4 kg (P = 0.001 and P = 0.034, respectively). However, final weight, average daily gain, average daily intake and feed conversion efficiency were not statistically affected by dietary treatment during the starter (<35.8 kg), fattening period (101.4–157.9 kg) or overall (16.3–157.9 kg). Retinol concentration in tissues reflected the dietary vitamin A level. Retinol and retinyl palmitate accumulation in hepatic and fat depots of control animals were more marked during the growing than during the finishing period. Retinol depots decreased in restricted groups and showed different sensitivity for mobilization between tissues, with faster retinol mobilization from the liver. The ER group had a higher hepatic α-tocopherol increase than the LR group (P < 0.0001). However, in fat the increase in α-tocopherol levels were more marked in the LR than in the ER group (P < 0.0001). ADH1C gene expression was higher (P = 0.0237) in CONTROL than in ER at 101 kg and LRAT gene expression showed a dose-dependent decrease in the ER group at 101 and 158 kg LW (P < 0.0001). There were no differences in RBP4, ALDH1A1, MTTP and TTP gene expression as affected by dietary treatment. Growth time influenced gene expression, with ADH1C and RBP4 genes being mainly expressed at 101 kg LW compared to pigs at 36 kg or 158 kg LW (P < 0.05). Relative expression of MTTP and TTP was also affected by time and showed an opposite pattern to that observed for vitamin A-related genes. The results suggest that removing vitamin A from the diet for long or short periods in heavy pigs has the potential to reduce feed costs, increasing tissue α-tocopherol levels without affecting slaughter weight or feed efficiency.
    Animal Feed Science and Technology 01/2015; 202. DOI:10.1016/j.anifeedsci.2015.01.014 · 2.09 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A procedure to quantify intramuscular fat was developed using common inexpensive laboratory equipment. Three homogenization methods of lyophilized muscle samples (Ball-mill, Grinder and Mortar) and two extraction methods (Ball-mill or Vortex) were used in turkey meat and pork. Two-hundred mg of lyophilized and homogenized samples were accurately weighed and mixed with 1.5mL of dichloromethane-methanol (8:2) and shaken either in a Mixer Mill (MM400, Retsch Technology) or in a Vortex. The final mixture was separated by centrifugation. Solvent was evaporated under a nitrogen stream and lipid content was gravimetrically determined. Besides, it was checked that the fatty acid profile was not altered by the protocol used. Moreover, the analysis of 4 replicas from the same sample showed different variation coefficients (16-29%) for the new procedures proposed over a wide range of IMF content. The combination of Grinder and Vortex methodologies can be proposed as a simple and inexpensive alternative to previous ones. Copyright © 2015. Published by Elsevier Ltd.
    Meat Science 01/2015; 103C:24-27. DOI:10.1016/j.meatsci.2014.12.011 · 2.23 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectifs Déterminer la faisabilité de l’IRM pour la réalisation d’imageries de la graisse sous-cutanée et viscérale au cours d’études longitudinales sur des modèles porcins d’obésité (porc ibérique) in vivo. Établir les régions d’intérêt (RDI) anatomiques et les points de mesure (PM) adéquats afin de les évaluer par des analyses portant sur la variabilité inter-individuelle et leur reproductibilité au cours du temps et par l’évaluation de leur fiabilité et de leur validité par rapport à des données zoométriques in vivo et ex vivo. Matériels et méthodes Cinq porcs mâles et cinq porcs femelles âgés de quatre à huit mois ont été utilisés. L’IRM a été effectuée avec un appareil Panorama de 0,23 T (Philips Medical Systems, Best, Pays-Bas), en utilisant une antenne corps/rachis XL (body/spine XL coil). Résultats Les RDI de choix pour la visualisation des données sous-cutanées sont situées entre le bord cranial du pilier gauche du diaphragme et la vertèbre lombaire L3. L’adiposité viscérale peut également être évaluée des vertèbres L1 à L3. Conclusions L’IRM permet l’évaluation de la graisse sous-cutané et viscérale lors d’une seule acquisition, ce qui améliore le bien-être animal ainsi que les rapports temps–efficacité et coût–efficacité, et permet de réaliser une procédure précise, cohérente et répétable pour des études ultérieures de l’adiposité chez des porcs obèses.
    09/2014; DOI:10.1016/j.jradio.2013.12.023
  • [Show abstract] [Hide abstract]
    ABSTRACT: Maternal malnutrition during pregnancy, both by deficiency and excess, induces changes in the intrauterine environment and the metabolic status of the offspring, playing a key role in the growth, status of fitness/obesity and appearance of metabolic disorders during postnatal life. There is increasing evidence that these effects may not be only limited to the first generation of descendants, the offspring directly exposed to metabolic challenges, but to subsequent generations. The present study evaluated, in a swine model of obesity/leptin resistance, the existence and extent of transgenerational developmental programming effects. Pre- and postnatal development, adiposity and metabolic features were assessed in the second generation of piglets descendant of sows exposed to either under- or overnutrition during pregnancy. The results indicated that these piglets showed early-postnatal increases in adiposity and disturbances in lipids profiles compatible with the early prodrome of metabolic syndrome, with liver tissue also evidencing pediatric liver disease. These features evidencing early-life metabolic disorders were more evident in the males that were descendant from overfed grandmothers and during the transition from milk to solid feeding. Thus, the present study gives evidence supporting transgenerational developmental programming and supports the necessity of the development of strategies for avoiding the current epidemics of childhood overweight and obesity.
  • Argimiro Daza, Ana I. Rey, Celia Lopez-Carrasco, Clemente J. Lopez-Bote
    [Show abstract] [Hide abstract]
    ABSTRACT: The effect of gender (entire males vs females) on growth performance, carcass traits and meat and fat quality of Avilena-Negra Iberica calves, fattened under free range conditions and supplemented with concentrate, were investigated. The average daily gain, leg width and thorax depth were higher in males than in females. Carcass conformation score, fore-quarter weight and percentage in carcass were higher in males than in females, whereas dressing percentege, loins and flank percentages in carcass and carcass fatness degree were higher in females than in males. Instrumental colour variables of muscle were not affected by gender at days 1, 3, 7 and 9 of refrigerated storage. In muscle, the L*, a*, b*, chroma, oxymyoglobine and oxymyoglobine/metmyoglobine ratio values observed 9 days after slaughter were lower than those at days 1 and 3 after slaughter. Intramuscular fat percentage of Longissimus thoracis muscle was higher in females than in males. Gender had no effect on alpha-tocopherol content in intramuscular fat (IMF) from Longissimus thoracis muscle. The omental and IMF of females had lower C18:2 n-6, Sigma n-6 and Sigma PUFA proportions than those from the males. In IMF C16:1, C18:1 n-9 and Sigma MUFA proportions were greater in females than in males. The IMF percentage in Longissimus thoracis affected significantly to its fatty acid composition.
    08/2014; 12(3):683. DOI:10.5424/sjar/2014123-4693
  • [Show abstract] [Hide abstract]
    ABSTRACT: In total, 540 crossbred pigs with an initial body weight of 28.5kg were used to investigate the effects of the net energy (NE) content (2.29, 2.33, 2.37, 2.41 and 2.45 Mcal/kg) of the diet on growth performance and carcass and meat quality traits of gilts, boars and immunocastrated males (IMC). An increase in dietary NE increased NE intake and decreased feed conversion ratio linearly. The IMC pigs showed greater feed intake and average daily gain than gilts and boars. Backfat depth increased and chilled and trimmed ham yield decreased, as the dietary NE increased. Backfat depth was greater for gilts and IMC than for boars. Also, gilts had greater carcass and loin yields than boars and IMC. Diets with the greater NE content were more appropriate for the production of heavy pigs. However, the economic interest of this practice needs further assessment.
    Meat Science 08/2014; 98(4):773-780. DOI:10.1016/j.meatsci.2014.07.025 · 2.23 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: We aimed to detefinine whether the response of linoleic acid (C18:2 n-6) tissue concentration to dietary C18:2 n-6 administration, in the finishing period, is done in a different way in Iberian pig compared with a lean genotype. Therefore, 48 pigs, 24 of a lean genotype (Large White x Great York) and 24 of a fat genotype (Iberian x Duroc) were offered three commercial diets, for each genetic type, in order to provide three levels of C18:2 n-6, but maintaining constant the concentration of saturated and linolenic fatty acids (FA) in each genotype. Samples from adipose tissue (subcutaneous backfat and intramuscular fat (IMF) from Longissimus dorsi muscle) were taken. Six pigs of each genotype (two per each C18:2 n-6 level) were slaughtered at the start of the trial to estimate initial fatty acids composition. The C18:2 n-6 proportions at slaughter were higher in the subcutaneous backfat outer layer than in subcutaneous backfat inner layer for both genetic types. In both backfat layers, as the dietary C18:2 n-6 increased, the C18:2 n-6 proportions also increased in both genotypes. In IMF, the concentration of C18:2 n-6 was also altered in lean genotype according to dietary treatment, but no response was observed in Iberian pigs. Linoleic acid concentrations was higher in lean pig genotypes than in the Iberian pig, both in subcutaneous and IMF throughout the whole range of dietary linoleic acid concentration used in this experiment. These results indicate the resistance of Iberian pig to modify the proportion of linoleic in their tissues, particularly in the IMF.
    06/2014; 12(2):419. DOI:10.5424/sjar/2014122-4361
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The two main genetic types in Iberian pig production show important phenotypic differences in growth, fattening and tissue composition since early developmental stages. The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. Contemporary families coming from pure Iberian pigs (IB) or from crossing with Duroc boars (DUxIB) were generated. Piglets (14 from each genetic type) were slaughtered at weaning (28 days) and longissimus dorsi was sampled for composition and gene expression studies. RNA was obtained and hybridized to Affymetrix Porcine Genechip expression arrays.
    BMC Genomics 05/2014; 15(1):413. DOI:10.1186/1471-2164-15-413 · 4.04 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To determine the feasibility of MRI for imaging subcutaneous and visceral adiposity in longitudinal studies in obese swine models (Iberian pig). To establish the anatomical regions of interest (ROIs) and measurement points (MPs) adequate for their evaluation through analyses on the inter-individual variability and over-time reproducibility and through the assessment of their reliability and validity by comparison with in vivo and ex vivo zoometric data. Five male and five female pigs were used from four (live weight around 48kg and back-fat depth around 18mm) to eight months old (live weight 134kg and back-fat depth around 48mm). MRI was carried out with a Panorama 0.23T scanner (Philips Medical Systems, Best, Netherlands), using a body/spine XL coil. The ROIs of election for visualization of subcutaneous data are located from the cranial margin of left diaphragmatic crura to the lumbar vertebrae L3. Visceral adiposity may be equally evaluated from the vertebrae L1 to L3. MRI allows the evaluation of subcutaneous and visceral fatness in a single acquisition, which improves animal welfare and time- and cost-efficiency and provides an accurate, consistent and repeatable procedure for sequential studies of adiposity in obese swine.
    04/2014; 95(9). DOI:10.1016/j.diii.2014.03.002
  • Source
    D Amazan, G Cordero, C J López-Bote, C Lauridsen, A I Rey
    [Show abstract] [Hide abstract]
    ABSTRACT: This study evaluated the strategy of supplementing oral micellized natural vitamin E (d-α-tocopherol) to either piglets and/or sows on α-tocopherol concentrations in piglets serum and tissues after weaning. One first experiment tested the influence of the vitamin E supplementation source (natural form in water v. the synthetic form in feed) and dose administered to piglets and/or sows on serum α-tocopherol concentration, α-tocopherol stereoisomer accumulation, antioxidant capacity and immune response of weaned piglets. A second experiment studied the effect of sow source and dose vitamin E supplementation on some of these parameters in piglets. Oral supplementation to sows with natural vitamin E as a micellized form (d-α-tocopherol) at the lowest dose produced a similar concentration of α-tocopherol in serum at days 2, 14 and 28 postpartum to those supplemented with threefold higher dose of the synthetic form in feed. At day 39 of age, neither piglet supplementation source nor dose significantly affected α-tocopherol accumulation in the serum, muscle, subcutaneous fat or liver. Those piglets from sows supplemented with the micellized alcohol form had higher RRR-α-tocopherol stereoisomers (P<0.001) and lower (P<0.001) RRS- RSS- and RSR-α-tocopherol, at day 39 of age than those from sows supplemented with the synthetic form. A predominant importance of sow over piglet vitamin E supplementation was observed on stereoisomer distribution in piglets. Low doses of oral natural vitamin E supplementation to sows or piglets did not increase the oxidative stress of piglets when compared with the use of the synthetic form in feed. Immunoglobulin levels in piglet serum at day 39 were not affected by natural vitamin E supplementation at low doses in drinking water of piglets or sows when compared with the synthetic form in feed. IgA tended to be higher (P=0.145) at day 39 in piglets supplemented with natural vitamin E when compared with those supplemented with the synthetic form. Low doses of oral micellized natural vitamin E supplementation to sows is an interesting feeding strategy, when compared with the use of high doses of the synthetic form in feed, because it results in similar α-tocopherol concentrations, allows a predominant -R stereoisomer distribution in piglets and also maintains their oxidative status in vivo.
    animal 03/2014; 8(3):410-9. DOI:10.1017/S1751731113002401 · 1.78 Impact Factor
  • J Segura, C J Lopez-Bote
    [Show abstract] [Hide abstract]
    ABSTRACT: A new procedure to extract intramuscular fat (IMF) was developed to minimize sample amount, solvent use and time of analysis. Lyophilised samples (200mg) were accurately weighed in a safe-lock micro test tube, homogenized in 1.5mL dichloromethane-methanol (8:2) and mixed in a mixer mill (MM400, Retsch technology). The final biphasic system was separated by centrifugation (8min, 10,000rpm). The extraction was repeated three times. Solvent was evaporated under nitrogen stream and lipid content was gravimetrically determined. Results from 64 determinations were compared to those obtained with other referred method and showed a linear response over the whole range of IMF content (1.6-6.9g/100g sample). Moreover, the analysis with different methodology of six replica from the same sample showed lowest variability (standard deviation intra-method) for the new methodology proposed over a wide range of IMF content. A cost and time efficient lipid extraction procedure was developed without loss of precision and accuracy and with a fatty acid profile comparable to other protocols.
    Food Chemistry 02/2014; 145C:821-825. DOI:10.1016/j.foodchem.2013.08.131 · 3.26 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Maternal energy restriction during pregnancy predisposes to metabolic alterations in the offspring. The present study was designed to evaluate phenotypic and metabolic consequences following maternal undernutrition in an obese pig model and to define the potential role of hypothalamic gene expression in programming effects. Iberian sows were fed a control or a 50 % restricted diet for the last two-thirds of gestation. Newborns were assessed for body and organ weights, hormonal and metabolic status, and hypothalamic expression of genes implicated in energy homeostasis, glucocorticoid function and methylation. Weight and adiposity were measured in adult littermates. Newborns of the restricted sows were lighter (P <0·01), but brain growth was spared. The plasma concentration of TAG was lower in the restricted newborns than in the control newborns of both the sexes (P <0·01), while the concentration of cortisol was higher in females born to the restricted sows (P <0·04), reflecting a situation of metabolic stress by nutrient insufficiency. A lower hypothalamic expression of anorexigenic peptides (LEPR and POMC, P <0·01 and P <0·04, respectively) was observed in females born to the restricted sows, but no effect was observed in the males. The expression of HSD11B1 gene was down-regulated in the restricted animals (P <0·05), suggesting an adaptive mechanism for reducing the harmful effects of elevated concentrations of cortisol. At 4 and 7 months of age, the restricted females were heavier and fatter than the controls (P< 0·01). Maternal feed restriction induces asymmetrical growth retardation and metabolic alterations in the offspring. Differences in gene expression at birth and higher growth and adiposity in adulthood suggest a female-specific programming effect for a positive energy balance, possibly due to overexposure to endogenous stress-induced glucocorticoids.
    The British journal of nutrition 02/2014; 111(4):735-46. DOI:10.1017/S0007114513002948 · 3.34 Impact Factor
  • A Daza, M A Latorre, A Olivares, C J López-Bote
    [Show abstract] [Hide abstract]
    ABSTRACT: A total of 48 Duroc×(Landrace×Large White) gilts of 33.2 kg BW were used to investigate the influence of immunocastration and diet on growth performance and carcass, meat and fat quality. Four treatments were arranged factorially (2×2) with two sexes (immunocastrated gilts: IG v. entire gilts: EG) and two dietary treatments (a commercial feedstuff as control v. granulated barley as a single major ingredient) provided during the finishing period (from 103 to 126 kg BW). There were four replicates of three pigs per treatment. At the end of the trial, the IGs grew faster (P<0.05) and ate more feed (P<0.05) than the EGs. Carcasses from the IGs had thicker backfat depth than those from the EGs (P<0.01) and carried out a lower percentage of rejected carcasses (P<0.05) at slaughterhouse owing to lack of fat. Meat from the IGs tended to have higher intramuscular fat (IMF) content in the Longissimus thoracis (LT) muscle than that from the EGs (P=0.09). In addition, immunocastration increased the total saturated fatty-acid proportion in subcutaneous fat and IMF (P<0.001) and decreased the total monounsaturated fatty acid (MUFA) and total polyunsaturated fatty acid (PUFA) percentages in subcutaneous backfat (P<0.05 and P<0.001, respectively) and in IMF (P<0.01 and P=0.06, respectively). The use of a diet based on granulated barley during the finishing period had no effect on growth performance but tended to increase IMF content in the LT muscle (P<0.06), and increased MUFA (P<0.05) and decreased PUFA (P<0.01) proportions in omental fat. It is concluded that immunocastration of gilts intended for dry-cured ham industry improved some aspects of growth performance and carcass and meat quality, whereas granulated barley had scarce effect on productive traits and fatty-acid profile but tended to improve IMF content.
    animal 01/2014; 8(3):1-10. DOI:10.1017/S1751731113002292 · 1.78 Impact Factor
  • Source
    A.I. Rey, C J López-Bote
    [Show abstract] [Hide abstract]
    ABSTRACT: The use of stereoisomers of α-tocopherol to correctly classify Iberian pig fat samples according to their feeding system was investigated. Samples were obtained over two different seasons in controlled farms from the four categories of pigs described in the Industry Quality Policy (FREE-RANGE: pigs fed exclusively under free-range conditions; FREE-FEED: pigs fed free-range and supplemented with feed; FEED-OUT: pigs fed outdoors with access to grass and a mixed diet; and FEED: pigs fed exclusively a mixed diet). A higher presence of RRR-stereoisomer indicated a greater consumption of the natural form of tocopherol provided by acorns or grass, whereas a higher proportion of S forms were related to a higher mixed diet intake. Validation results showed 90% success in fat sample classification. Analysis of the RRR-stereoisomer together with γ- and α-tocopherol determination can be considered as a potent tool for distinguishing fat from pigs fed under free-range conditions or exclusively with acorns and grass from those receiving a supplemented diet at any time of their fattening phase.
    Food Chemistry 01/2014; 142:342-8. DOI:10.1016/j.foodchem.2013.07.055 · 3.26 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study aimed to determine, in a swine model of leptin resistance, the effects of type and timing of maternal malnutrition on growth patterns, adiposity and metabolic features of the progeny when exposed to an obesogenic diet during their juvenile development and possible concomitant effects of the offspring sex. Thus, four groups were considered. A CONTROL group involved pigs born from sows fed with a diet fulfilling their daily maintenance requirements for pregnancy. The treated groups involved the progeny of females fed with the same diet but fulfilling either 160% or 50% of pregnancy requirements during the entire gestation (OVERFED and UNDERFED, respectively) or 100% of requirements until Day 35 of pregnancy and 50% of such amount from Day 36 onwards (LATE-UNDERFED). OVERFED and UNDERFED offspring were more prone to higher corpulence and fat deposition from early postnatal stages, during breast-feeding; adiposity increased significantly when exposed to obesogenic diets, especially in females. The effects of sex were even more remarkable in LATE-UNDERFED offspring, which had similar corpulence to CONTROL piglets; however, females showed a clear predisposition to obesity. Furthermore, the three groups of pigs with maternal malnutrition showed evidences of metabolic syndrome and, in the case of individuals born from OVERFED sows, even of insulin resistance and the prodrome of type-2 diabetes. These findings support the main role of early nutritional programming in the current rise of obesity and associated diseases in ethnics with leptin resistance.
    PLoS ONE 10/2013; 8(10):e78424. DOI:10.1371/journal.pone.0078424 · 3.53 Impact Factor

Publication Stats

2k Citations
190.40 Total Impact Points

Institutions

  • 1997–2015
    • Complutense University of Madrid
      • • Department of Animal Production
      • • Facultad de Veterinaria
      • • Departamento de Bioquímica y Biología Molecular IV
      Madrid, Madrid, Spain
  • 2005
    • Universidad Politécnica de Madrid
      • Departamento de Producción Animal
      Madrid, Madrid, Spain
  • 1998
    • Michigan State University
      • Department of Food Science and Human Nutrition
      East Lansing, MI, United States
  • 1988–1994
    • Universidad de Extremadura
      • Faculty of Veterinary
      Ara Pacis Augustalis, Extremadura, Spain