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Publications (13)12.85 Total impact

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    ABSTRACT: The single nucleotide polymorphisms (SNPs) in the 5' upstream of bovine IL8 gene were investigated in 810 Chinese Holstein cows from 35 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. The Real-time PCR and Western blot were used to detect the mRNA and protein levels of genotype Chinese Holstein dairy cows. The results showed that one SNP -105G>A was detected, designating three genotypes (GG, GA and AA) with respective frequencies of 0.38, 0.46, and 0.16. The significant association of the SNP -105G>A with somatic cell score (SCS) was identified. Genotype GG had a significantly lower SCS than genotype GA or AA (P < 0.01), and the relative mRNA expression and protein level of GG was found to be the highest. These results suggest that the genotype GG may be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein dairy cows.
    Animal Biotechnology 04/2015; 26(2):143-147. · 0.88 Impact Factor
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    ABSTRACT: Mastitis is one of the most prevalent diseases in dairy cattle; CXCR1 plays a key role in mastitis resistance via IL8 signaling pathway, with the CXCR1 SNPs showing a different degree of mastitis resistance. To investigate the situation of CXCR1 polymorphisms in Chinese Holstein cattle and determine the relationship between the CXCR1 SNPs and mastitis resistance, the CXCR1 SNPs in 610 Chinese Holstein cattle of 30 families were investigated using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. The results showed that four SNPs, -1830A > G, -1768T > A, -344T > C, and 783C > A were detected at 5' upstream and coding region. The correlation analysis demonstrated that -1830AA, -1768TT, and -344TT correlated significantly with the lowest SCS for each site, respectively. Haplotype analysis revealed Haplo2 (ATTA) correlated significantly with the lowest SCS. These findings indicated a prospect genetic marker of mastitis resistance in dairy cattle.
    Animal Biotechnology 07/2011; 22(3):133-42. · 0.88 Impact Factor
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    ABSTRACT: To explore the relation of Interleukin-8 (IL8) gene polymorphism with immunity against mastitis in dairy cow, the polymorphism of IL8 gene was investigated in 610 Chinese Holstein cow from 30 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique, and milk yield, milk fat percentage, milk protein percentage, 305 day corrected milk yield, 305 day milk fat yield, 305 day milk protein yield and somatic cell score (SCS) were measured and analyzed, and the mRNA levels of IL8 genotypes in blood were detected by real-time PCR. The results showed that three genotypes, KK, KA and AA were detected with frequencies of 0.187, 0.451, and 0.362, respectively. The gene frequencies of K and A were 0.412 and 0.588, respectively. The significant association of the IL8 mutations with milk yield, 305 day milk protein yield, 305 day corrected milk yield and 305 day milk fat yield, SCS, and significant association with milk protein percentage were identified, while their association with milk fat percentage were not significant. KK had higher milk yield, 305 day milk protein yield, 305 day corrected milk yield and 305 day milk fat yield than AA or KA, and the least square mean of SCS of KK was significantly lower than that of AA or KA. AA had significant lower milk protein yield than KK or KA. The relative IL8 mRNA level of KK in blood was the highest. These findings demonstrated that IL8 genotype significantly correlated with mastitis resistance and the locus could be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein.
    Molecular Biology Reports 11/2010; 38(6):4083-8. · 2.51 Impact Factor
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    ABSTRACT: The level of genetic differentiation, gene flow and the relationship between geographical distance and genetic differentiation among six sheep populations of Mongolian group in China (Tong sheep, small-tailed Han sheep, Hu sheep, Tan sheep, Ujumuqin sheep and Bayinbuluk sheep) were analyzed using seven microsatellites. The trees were constructed from diversity coefficient (DC) distances among the six sheep populations. The overall heterozygote deficit across all the populations (F it) was between 0.167 (OarAE101) and 0.044 (MAF33). The over-all significant deficit of heterozygote, because of inbreeding within breeds, (F is) was between 0.089 (OarFCB304) and 0.005 (MAF33). The coefficient of genetic differentiation (F st) was between 0.100 (OarAE101) and 0.022 (OarFCB48). It indicated that 3.9% of the total genetic variation could be explained by breed differences and the remaining 96.1% by differences among individuals for each population. This illustrated that most variations existed within breeds and genetic differentiation level were very low among sheep breeds of the Mongolian Group in China. The average number of effective migrants exchanged per generation (Nem) ranged from 2.7369 (Tan sheep and Bayinbuluk sheep) to 44.3928 (Tong sheep and Hu sheep), and the mean value was 11.25213. Significantly positive relationships between the level of genetic differentiation and geographical distance and genetic distances were detected. It is concluded that genetic differentiation of sheep breeds of Mongolian group in China is mainly the result of natural selection (different living conditions).
    Frontiers of Agriculture in China 08/2008; 2(3):338-342.
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    ABSTRACT: A total of 321 individuals from six cattle populations of four species in a bovine subfamily in China were studied using 12 pairs of microsatellite markers. The genetic diversities within and between populations were calculated. The phylogenetic trees were constructed by (delta mu)(2) and DA distances, and the divergence times between populations were estimated by (delta mu)(2). Altogether, 144 microsatellite alleles were detected including 24 private alleles and nine shared alleles. Chinese Holstein had the largest number of private alleles (10), whereas, Bohai black and Buffalo had the smallest number of private alleles (2). Chinese Holstein showed the highest genetic variability. Its observed number of alleles (Na), mean effective number of alleles (MNA), and mean heterozygosity (He) were 7.7500, 4.9722, and 0.7719, respectively, whereas, the Buffalo and Yak showed low genetic variability. In the phylogenetic trees, Luxi and Holstein grouped first, followed by Bohai and Minnan. Yak branched next and buffalo emerged as the most divergent population from other cattle populations. Luxi and Bohai were estimated to have diverged 0.039-0.105 million years ago (MYA), however, buffalo and Holstein diverged 0.501-1.337 MYA. The divergence time of Yak versus Minnan, Holstein and buffalo was 0.136-0.363, 0.273-0.729, and 0.326-0.600 MYA, respectively.
    Journal of Genetics and Genomics 02/2008; 35(1):25-32. · 2.08 Impact Factor
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    ABSTRACT: This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin (Tf), alkaline phosphatase (Alp), leucine aminopeptidase (Lap), arylesterase (Ary-Es), hemoglobin-beta (Hb-beta), X-protein (X-p), carbonic anhydrase (CA), catalase (Cat), malate dehydrogenase (MDH), and lysine (Ly), whereas, albumin (Al) and postalbumin (Po) loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the "Mongolia group", and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.
    Journal of Genetics and Genomics 01/2008; 34(12):1097-105. · 2.08 Impact Factor
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    ABSTRACT: The genomes of six populations were screened using microsatellites as molecular markers, including Ujmuqin sheep, small-tailed Han sheep, Tan sheep, Hu sheep, Tong sheep and Yangtse River Delta (YRD) white goat. A total of seven microsatellite markers were used and genetic diversity and genetic distance were also determined. The results showed that there were 224 alleles in six populations, all seven loci showed polymorphism in all populations. The average heterozygosity of all populations was 0.949 9, and the mean polymorphism information content (PIC) of all six populations was 0.842 5–0.929 4. The six sheep (goat) popualtions were lowly differentiated with all loci, and the coefficient of phaenotype differentiation (Fst) was 2.6%, which was consistent with the coefficient of gene differentiation (Gst). The global heterozygote deficit across of all populations (Fit) amounted to 0.5%. The overall significant deficit of heterozygotes because of inbreeding within breeds (Fis) amounted to −2.2%. Two Unweighted Pair-group Method using Arithmetic Averages (UPGMA) dendrograms were constructed on the basis of Nei’s standard genetic distance (DS) and Nei’s genetic distance (DA) respectively. Hu sheep and Tong sheep were grouped at first, Ujmuqin sheep and small-tailed Han sheep clustered and then clustered with Tan sheep. Finally, Yangtse River Delta white goat joined in with all above. From this study, Ujmuqin sheep belongs to “Mongolia sheep” group, which corresponds with the historical records exactly. Ujmuqin sheep and small-tailed Han sheep, Tan sheep, Hu sheep and Tong sheep all vest in the “Mongolia sheep” group.
    Frontiers of Agriculture in China 09/2007; 1(4):472-477.
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    ABSTRACT: Variations of four sheep populations in China were examined by multiloci electrophoresis, and similar data are quoted to analyze the degree of genetic differentiation of native sheep populations in East and South Asia. Among 15 populations, the average heterozygosity is 0.2746, and the effective number of alleles is 1.559. Mongolian sheep possess the highest genetic diversity, and diversity decreases sequentially in the Chinese, Vietnamese, Bangladeshi, and Nepalese populations. Coefficients of genetic differentiation are 0.0126-0.3083, with an average of 0.148, demonstrating that the major genetic variation (85%) exists within populations. Genetic identity and genetic distance all show relatively low genetic differentiation. No relationship was found between geographic distance and genetic distance. Gene flow is common among the mass of populations, which leads to the inconsistency between geographic distance and genetic distance. The 15 native sheep populations in East and South Asia can be divided into two groups, one group including part of the Chinese and Mongolian populations and another including the Yunnan population of China and part of the Nepalese and Bangladeshi populations. Other populations did not separate into groups, merging instead into the two main groups.
    Biochemical Genetics 05/2007; 45(3-4):263-79. · 0.94 Impact Factor
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    ABSTRACT: Levels of genetic differentiation, gene flow, and genetic structure of three indigenous cattle populations (Luxi, Bohai, and Minnan) and two reference cattle populations (Chinese Holstein and Qinhai yak) in China were estimated using the information from 12 microsatellites, and 141 microsatellite alleles were identified. The mean number of alleles per locus ranged from 2.9005 in yak to 4.9722 in Holstein. The observed heterozygosity ranged from 0.5325 (yak) to 0.7719 (Holstein); 29 private alleles were detected. The global heterozygote deficit across all populations amounted to 58.5% (p < 0.001). The overall significant (p < 0.001) deficit of heterozygotes because of inbreeding within breeds amounted to 43.2%. The five cattle populations were highly differentiated (F (st) = 26.9%, p < 0.001) at all loci. The heterozygote deficit within the population was highest in Luxi cattle and lowest in yak. The average number of effective migrants exchanged per generation was highest (1.149) between Luxi and Holstein, and lowest (0.509) between Luxi and yak. With the application of prior population information, cluster analysis achieved posterior probabilities from 91% to 98% of correctly assigning individuals to populations. Combining the information of cluster analysis, gene flow, and Structure analysis, the five cattle populations belong to three genetic clusters, a taurine (Luxi and Chinese Holstein), a zebu (Bohai and Minnan), and a yak cluster. This indicates that Bohai black is closer to Bos indicus than Luxi cattle. The evolution and development of three indigenous cattle populations are discussed.
    Biochemical Genetics 04/2007; 45(3-4):195-209. · 0.94 Impact Factor
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    ABSTRACT: The genomes of seven native goat populations were screened by using microsatellites as molecular markers; the populations were Yichang white, Matou, Xiangdong black, Fuqing, Daiyun, Huanghuai and Yangtse River Delta white goats. A total of 23 microsatellite markers were used and genetic diversities and genetic distances were also determined. The results showed that only 21 loci showed polymorphism in all populations. BM0203 was a homozygotic locus in every population, but more than one allele was found among all populations. Alleles of BM6444 were homozygotic in Xiangdong Black and Yichang White goats, but more than one allele was detected in other populations. Average heterozygosity of all populations was 0.819 0, and the mean polymorphism information content (PIC) of all seven populations was 0.630 5–0.691 9. An unweighted pair group method with arithmetic mean (UPGMA) dendrogram was constructed on Nei’s standard genetic distance. Matou and Xiangdong black were grouped at first, then Fuqing, Daiyun, Yangtse River Delta white and Huanghuai goats joined them respectively. Finally, Yichang white goats clustered with all of the above.
    Frontiers of Agriculture in China 01/2007; 1(3):324-328.
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    ABSTRACT: Genetic coadaptability of wild Japanese quail, wild Common quail and Domestic quail populations in China was studied using 7 microsatellite DNA markers and Monte Carlo method to test genetic disequilibrium. The molecular effects of genetic coadaptability were analyzed through a new statistical model of neutral site. The results showed that genetic coadaptability dominated the genetic disequilibrium of the three quail populations, and totally 16.67%, 9.66% and 10.05% of non-allelic combinations were in the genetic disequilibrium in wild Japanese quail, wild Common quail and Domestic quail populations, respectively. Genetic coadaptability existed at almost all the tested sites. In the molecular point of view, genetic coadaptability plays an important role of keeping lots of polymorphisms in natural populations. Therefore, it is another key factor to the genetic disequilibrium in the population except for linkage. The results enrich the conceptions and connotations of genetic disequilibrium, and help us know more about genetic coadaptability and its effects, and lay a foundation of evaluation and protection of wild quail genetic resources in China.
    Science in China Series C Life Sciences 05/2006; 49(2):172-81. · 1.61 Impact Factor
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    ABSTRACT: Using the method of random sampling in typical colonies of the central area of the habitat and several electrophoresis techniques, the variations of 12 structural loci encoding blood protein of 60 Small-tailed Han sheep were examined and compared with those of four other sheep populations from coastal areas in East Asia to explore their phylogenetic relationships. Average heterozygosity of the five populations was: Kharkhorin sheep 0.3447, Ulaanbaatar sheep 0.3285, Small-tailed Han sheep 0.3157, Hu sheep 0.3884, and Cham Tribe sheep 0.2300. The earlier researchers' conclusion through documentary research, indicating that Small-tailed Han sheep and Hu sheep both evolved from Mongolian sheep, was further verified by the results of this study. Hu sheep, Small-tailed Han sheep, and Cham Tribe sheep were decreasingly affected by the bloodline of Mongolian sheep. A 1partial founder population, i.e., Mongolian sheep forming current Small-tailed Han sheep, possibly made a contribution to the bloodline of Cham Tribe sheep.
    Biochemical Genetics 07/2005; 43(5-6):251-60. · 0.94 Impact Factor
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    ABSTRACT: Based on the previous research, according to the Hierarchy clustering, the sheep populations in East and South Asia were divided into 3 groups: Mongolia group, South-Asia group and European group. In this study, the extraction of sheep was demonstrated by two discriminants based on fuzzy discriminants analysis. The discriminants were verified with sheep populations presented 34 alleles in 10 loci. The result showed the second discriminant might be the best known discriminant, which is derived from the theroy of genetic approach degree formula: 1 2 () = 1-() = 1-() () ~~ ~ K j j ~ ~ j , , | P P | I X K I X I X ξ µ µ = − Where () Pj I ~ µ is the membership function of allele No, j is the group No I, () Pj x ~ µ is the allele No, j is to distinguished population X,viz., the gene frequencies of allele j in group I, in population X, respectively; K is the number of allele; (X I ~ ,) is the linear genetic fuzzy degree. The extraction of an unknown population was checked using the second discriminant, the result proved that the genetic approach degree was reliable for discriminant of extraction in East and South Asia, which also might reflect the course of extraction breeding of Tong Sheep, Hu Sheep and Yunnan Sheep in China, and of course, it might be extended to other genetic markers.