Małgorzata Lewandowska-Szumieł

Publications (8)18.35 Total impact

• Article: Bone tissue engineering - a field for new medicinal products?

  Małgorzata Lewandowska-Szumiel, Joanna Wójtowicz
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  ABSTRACT: It was only in December 2008 that the European Union regulated the approval procedure for tissue engineered products (TEPs). Due to this regulation, TEP is classified as an advanced therapy medicinal product and as such may be recognized as a tool in pharmaceutical biotechnology. This paper gives a short review of the concept, the experimental evaluation and the clinical potency of tissue engineering (TE), with a particular focus on bone tissue engineered products. After a period of great enthusiasm about TE at the end of the 20th century a slight disappointment followed in the early 2000s. The review refers also to the continuously growing scientific interest, accompanied by the still modest representation of TEPs on the medical market. Some remarks are given on a bench-to-clinic road, including criticism concerning data originating from animal experiments. An attempt is made to foresee the still promising future of bone tissue engineered products (BTEPs) in practical use.
  Current pharmaceutical biotechnology 09/2011; 12(11):1850-9. · 3.40 Impact Factor
• Article: Candidate bone-tissue-engineered product based on human-bone-derived cells and polyurethane scaffold.

  Piotr Woźniak, Monika Bil, Joanna Ryszkowska, Piotr Wychowański, Edyta Wróbel, Anna Ratajska, Grazyna Hoser, Jacek Przybylski, Krzysztof J Kurzydłowski, Małgorzata Lewandowska-Szumieł
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  ABSTRACT: Biodegradable polyurethanes (PURs) have recently been investigated as candidate materials for bone regenerative medicine. There are promising reports documenting the biocompatibility of selected PURs in vivo and the tolerance of certain cells toward PURs in vitro - potentially to be used as scaffolds for tissue-engineered products (TEPs). The aim of the present study was to take a step forward and create a TEP using human osteogenic cells and a polyurethane scaffold, and to evaluate the quality of the obtained TEP in vivo. Human-bone-derived cells (HBDCs) were seeded and cultured on polyurethane scaffolds in a bioreactor for 14 days. The TEP examination in vitro was based on the evaluation of cell number, cell phenotype and cell distribution within the scaffold. TEPs and control samples (scaffolds without cells) were implanted subcutaneously into SCID mice for 4 and 13 weeks. Explants harvested from the animals were examined using histological and immunohistochemical methods. They were also tested in mechanical trials. It was found that dynamic conditions for cell seeding and culture enable homogeneous distribution, maintaining the proliferative potential and osteogenic phenotype of the HBDCs cultured on polyurethane scaffolds. It was also found that HBDCs implanted as a component of TEP survived and kept their ability to produce the specific human bone extracellular matrix, which resulted in higher mechanical properties of the harvested explants when preseeded with HBDCs. The whole system, including the investigated PUR scaffold and the method of human cell seeding and culture, is recommended as a candidate bone TEP.
  Acta biomaterialia 11/2009; 6(7):2484-93. · 3.98 Impact Factor
• Article: Optimization of the structure of polyurethanes for bone tissue engineering applications.

  Monika Bil, Joanna Ryszkowska, Piotr Woźniak, Krzysztof J Kurzydłowski, Małgorzata Lewandowska-Szumieł
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  ABSTRACT: Polyurethanes containing 22-70 wt.% hard segments were developed and evaluated for bone tissue engineering applications. Aliphatic poly(ester-urethanes) were synthesised from poly(epsilon-caprolactone) diol with different molecular masses (M= approximately 530, 1250 and 2000 Da), cycloaliphatic diisocyanate 4,4'-methylenebis(cyclohexyl isocyanate) and ethylene glycol as a chain extender. Changes in macromolecule order with increasing hard segment content were observed via modulated differential scanning calorimetry. Depending on the hard segment content, a gradual variation in polyurethane surface properties was revealed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and static contact angle measurements. As the hard segments content increased the polyurethane surface exhibited more phase separation, a higher content of urethane moieties and higher hydrophilicity. The biocompatibility results indicated that proliferation of human bone-derived cells (HBDC) cultured in vitro improved with increasing hard segment content while the osteogenic potential of HBDC decreased with increasing hard segment content.
  Acta biomaterialia 09/2009; 6(7):2501-10. · 3.98 Impact Factor
• Article: Degradation of Engineering Materials – Implications to Regenerative Medicine

  Krzysztof J. Kurzydłowski, Małgorzata Lewandowska, Wojciech Święszkowski, Małgorzata Lewandowska-Szumieł
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  ABSTRACT: Materials in general, to some degree are susceptible to environmental degradation. The degradation of biomaterials is one of the most relevant issues in the field of regenerative medicine. In industrial practice, the degradation is always a negative phenomenon. In bioengineering, the degradation may be undesirable (e.g. corrosion of metallic implants, wear of artificial joint implant) or desirable (biodegradable devices and tissue engineering). In both cases, the knowledge of the kinetics of degradation is crucial for safe use of biocomponents. The methods for predicting remaining life commonly used in industrial practice will be presented in the context of biomaterials. Non destructive techniques for monitoring degradation will be discussed and some ideas about their application to bio-environments proposed.
  Macromolecular Symposia 07/2007; 253(1):1 - 9.
• Article: Chemical Surface Modifications of Titanium Implants

  Małgorzata Lewandowska, Monika Włodkowska, Radosław Olkowski, Agata Roguska, Beata Polak, Marcin Pisarek, Małgorzata Lewandowska-Szumieł, Krzysztof J. Kurzydłowski
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  ABSTRACT: In the present work, various surface modifications have been applied to titanium surface. The aim of the modifications was to improve cell adhesion and to determine their influence on the properties of titanium surface. The unmodified and modified surfaces were observed using SEM. Subtle changes in modified surface layer of titanium samples were examined using the Auger Electron Microanalysis and Photoelectron Spectroscopy. The properties of surfaces were evaluated by contact angle and roughness measurements. The results revealed large differences in morphology of Ti modified with different procedures whereas only minor differences in the chemistry of the surfaces were detected. Preliminary quantitative measurements (cell number, viability and differentiation) of the MG 63 osteoblast-like cells in the direct contact with the surface of the investigated materials show that both the not pre-treated titanium surface and the surfaces modified by the methods used in the this work are all well tolerated by the living cells. Within the experimental scatter all the surfaces provided good substrate for proliferation and growth of the cells.
  Macromolecular Symposia 07/2007; 253(1):115 - 121.
• Article: Polyester scaffolds with bimodal pore size distribution for tissue engineering.

  Stanislaw Sosnowski, Piotr Woźniak, Małgorzata Lewandowska-Szumieł
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  ABSTRACT: This paper presents a method for the preparation of porous poly(L-lactide)/poly[(L-lactide)-co-glycolide] scaffolds for tissue engineering. Scaffolds were prepared by a mold pressing-salt leaching technique from structured microparticles. The total porosity was in the range 70-85%. The pore size distribution was bimodal. Large pores, susceptible for osteoblasts growth and proliferation had the dimensions 50-400 microm. Small pores, dedicated to the diffusion of nutrients or/and metabolites of bone forming cells, as well as the products of hydrolysis of polyesters from the walls of the scaffold, had sizes in the range 2 nm-5 microm. The scaffolds had good mechanical strength (compressive modulus equal to 41 MPa and a strength of 1.64 MPa for 74% porosity). Scaffolds were tested in vitro with human osteoblast-like cells (MG-63). It was found that the viability of cells seeded within the scaffolds obtained using the mold pressing-salt leaching technique from structured microparticles was better when compared to cells cultured in scaffolds obtained by traditional methods. After 34 d of culture, cells within the tested scaffolds were organized in a tissue-like structure. Photos of section of macro- and mesoporous PLLA/PLGA scaffold containing 50 wt.-% of PLGA microspheres after 34 d of culture. Dark spots mark MG-63 cells, white areas belong to the scaffold. The specimen was stained with haematoxylin/eosin. Bar = 100 microm.
  Macromolecular Bioscience 07/2006; 6(6):425-34. · 3.89 Impact Factor
• Source

  Available from: A.U. Daniels

  Article: Experimental model for observation of micromotion in cell culture.

  Małgorzata Lewandowska-Szumieł, Krzysztof Sikorski, Andrzej Szummer, Janusz Komender, Marcin Kowalski, A U Daniels
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  ABSTRACT: It is known that the micromotion between implant and bone inhibits direct bone growth either on or into implant surfaces in vivo. Nevertheless, biocompatibility tests in vitro of biomaterials for bone/implant interfaces are mainly performed under static conditions. This work describes a dynamic, in vitro experimental simulation of the effect of mutual, small-scale implant surface-tissue displacement on adhered cells. Disks of simulated tissue (PVP hydrogel) were subjected to cyclic micromotion ranging from 0 at the center to 1000 microm at the periphery at approximately 13 Hz, relative to biomaterial surfaces or tissue culture polystyrene controls populated with human osteoblasts in standard tissue culture plate wells. The effect of the interfacial micromotion on the number of cells remaining attached was quantitated by XTT assay. The activity level of the remaining cells was determined by an alkaline phosphatase assay, and cell stress was evaluated by nitrogen assay. Significantly more cells (ANOVA) became detached from similarly prepared surfaces of titanium, hydroxyapatite, and alumina compared to the polystyrene control, and detachment from alumina was greater than for the other two materials. The activity of the remaining attached cells was lower as compared to the static (no micromotion) control but not significantly different among the biomaterials. All nitrogen assays were negative, suggesting minimal cell stress occurred. The method is proposed as a useful and discriminating in vitro tool for biocompatibility studies focused on cell adhesion to biomaterials under conditions related to those which exist at the implant/bone interface in vivo, and it allows subsequent studies of the still-viable cells by other methods.
  Journal of Biomedical Materials Research Part B Applied Biomaterials 03/2005; 72(2):379-87. · 2.15 Impact Factor
• Article: Osteogenic cell contact with biomaterials influences phenotype expression.

  Dorota Kudelska-Mazur, Małgorzata Lewandowska-Szumieł, Michał Mazur, Janusz Komender
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  ABSTRACT: Relationship between (1) osteoblast adhesion and spreading, and (2) phenotype expression was investigated. Cellular adhesion and spreading were estimated after short time (24 h), whereas proliferation and other osteoblast functions--after 7 days. Primary human osteogenic cells were seeded on the samples of titanium (T), surgical steel (S) and tissue culture polystyrene (PS), and incubated at 37 degrees C. After 24 h a number of samples were stained with crystal violet and Hoechst; the average single cell area (spreading) and adhering cell number was measured on each sample. The remaining cultures were supplemented with dexamethasone (10 nM) and beta-glycerophosphate (5 mM), and incubation was continued for 7 days. The cells on each sample were counted and the following tests were performed: XTT mitochondrial activity assay, total protein content, alkaline phosphatase activity (ALP), Sirius Red test for collagen, osteocalcin and calcium concentration. After 24 h significantly greater cell spreading (p < 0.05) and number (p < 0.05) were on T than on S. After 7 days significantly higher on T than on S were: ALP activity (p < 0.000001), collagen (p < 0.0015) and calcium concentration (p < 0.03). XTT results were bigger on S than on T. In control - XTT results were higher than on the metals; collagen and ALP were lower than on T, and calcium level was significantly lower than on T and S (p < 0.025). After 7 days there were no differences in cell number between T and S. Cell number (24 h) correlated with ALP activity (7 days) on steel (coefficient of correlation, CC = 0.866) and titanium (CC = 0.742). The spreading correlated on steel and on titanium with calcium concentration (CC = 0.645 on S, CC = 0.696 on T) and collagen level (CC = -0.638 on S, CC = -0.69 on T). CONCLUSIONS: Better conditions for osteoblast phenotype expression on T after 7 days of culture coincided with greater adhesion and spreading of cells after 24 h on T, as compared with S. The initial contact of cells with underlying surface may influence osteoblast functions and possibly, bone regeneration and implant osteointegration in vivo. Early cell spreading may be an indicator of further expression of osteoblast phenotype and may be important for application of osteogenic cells in reconstructive surgery.
  Cell and Tissue Banking 02/2005; 6(1):55-64. · 0.96 Impact Factor