-
[show abstract]
[hide abstract]
ABSTRACT: A thorough phenotypic and genotypic analysis of 150 strains belonging to the Mycobacterium terrae complex resulted in the identification of a number of previously unreported sequevars (sqvs.) within the species known to belong to the complex. In the species Mycobacterium arupense were detected 3 sqvs. in the 16S rRNA gene, 6 sqvs. in the hsp65 gene and 15 sqvs. in the rpoB gene; in Mycobacterium senuense 2 sqvs. were present in each of the three genetic regions; in Mycobacterium kumamotonense 4, 2 and 10 sqvs. respectively and in M. terrae 3, 4 and 6 sqvs. respectively were found. The extraneousness of Mycobacterium triviale with the M. terrae complex was confirmed. The limited utility of biochemical tests and of mycolic acids analyses for the differentiation of the members of M. terrae complex was confirmed as well. The survey allowed furthermore to recognize three previously undescribed species characterized by unique sequences in 16S rRNA, hsp65 and rpoB genes. Mycobacterium engbaekii sp. nov., a species proposed 40 years ago but never validly published, is characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it is related to Mycobacterium hiberniae. The type strains of this species, of which eight strains were investigated, is ATCC27353=DSM45694 A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense sp. nov. which has intermediate growth rate and is unpigmented, the nitrate reductase activity is typically strong. Closely related to M. arupense in the 16S rRNA gene, M. heraklionense is clearly differentiable from the latter species in the other genetic regions. The type strain is NCTC13432=LMG2473= CECT7509. Mycobacterium longobardum sp. nov., represented in the study from seven strains, is characterized by a unique phylogenetic location within the M. terrae complex, evidently divergent from any other species. The type strain is DSM45394=CCUG58460.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2012; · 2.11 Impact Factor
-
Enrico Tortoli,
Erik C Böttger,
Anna Fabio,
Enevold Falsen,
Zoe Gitti,
Antonella Grottola,
Hans-Peter Klenk,
Roberta Mannino, Alessandro Mariottini,
Massimino Messinò,
Monica Pecorari,
Fabio Rumpianesi
[show abstract]
[hide abstract]
ABSTRACT: Four strains isolated in the last 15 years were revealed to be identical in their 16S rRNA gene sequences to MCRO19, the sequence of which was deposited in GenBank in 1995. In a polyphasic analysis including phenotypic and genotypic features, the five strains (including MCRO19), which had been isolated in four European countries, turned out to represent a unique taxonomic entity. They are scotochromogenic slow growers and are genetically related to the group that included Mycobacterium simiae and 15 other species. The novel species Mycobacterium europaeum sp. nov. is proposed to accommodate these five strains. Strain FI-95228(T) ( = DSM 45397(T) = CCUG 58464(T)) was chosen as the type strain. In addition, a thorough revision of the phenotypic and genotypic characters of the species related to M. simiae was conducted which leads us to suggest the denomination of the 'Mycobacterium simiae complex' for this group.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 61(Pt 7):1606-11. · 2.11 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Microorganisms of the genus Methylobacterium are facultative methylotrophic, gram-negative rods that are ubiquitous in nature and rarely cause human disease, mostly in subjects with preexisting causes of immune depression. Methylobacterium fujisawaense, first proposed as a new species in 1988, has never been reported as a bacterial agent of human infections so far. Here we describe a case of M. fujisawaense infection in a relapsed acute leukaemia undergoing unrelated allogeneic hematopoietic stem cell transplantation. Molecular identification of an M. fujisawaense strain was obtained from multiple mycobacterial blood cultures.
Case Reports in Medicine 01/2010; 2010:313514.
-
[show abstract]
[hide abstract]
ABSTRACT: The treatment of multidrug-resistant tuberculosis (TB) requires the use, for long periods, of drugs liable to cause significant side effects. In the case of misdiagnosis of multidrug-resistant TB, the patient is exposed to toxic substances without any benefit. In low-income countries, where the microbiological diagnosis of TB relies on microscopy only, the misdiagnosis of multidrug-resistant TB is very frequent in patients persistently smear-positive despite anti-TB treatment, with the possibility of an infection due to non-tuberculous mycobacteria (NTM) being neglected. The isolation of a mycobacterium from the sputum of a Somali patient apparently confirmed the previous diagnosis of cavitary pulmonary disease. Preliminary investigations led, at first, to the strain being identified as multidrug-resistant Mycobacterium tuberculosis, with findings fully in agreement with the patient's history, which was characterized by repeated interruptions of anti-TB treatment. Thorough phenotypic and genotypic analyses led subsequently to the recognition that the strain was a previously unreported non-tuberculous mycobacterium. The patient, who was unresponsive to the anti-TB treatment, dramatically improved once a drug combination active against NTM was used. A major objective of this article is to alert the medical community to the risk, present also in settings in which sophisticated diagnostic techniques are used, that a cavitary infection due to NTM, and consequently not responding to the anti-TB standard regimen, will be mistaken for multidrug-resistant TB.
Clinical Microbiology and Infection 10/2009; 16(8):1130-4. · 4.54 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Five independent strains, isolated from clinical samples but probably not responsible for disease, revealed phenotypic and genotypic features that appeared to exclude their belonging to any of the recognized Mycobacterium species. The strains, which are non-pigmented rapid growers, presented a cell-wall lipid pattern resembling those of Mycobacterium brumae and Mycobacterium fallax. Sequencing of the 16S rRNA, hsp65 and rpoB genes and the 16S-23S rRNA gene internal transcribed spacer (ITS) revealed that the strains are clearly distinct from every other Mycobacterium species. While the 16S rRNA and rpoB genes were characterized by a single sequevar, two sequevars were detected in hsp65 and three in the ITS. The divergence shown in the latter region was striking, in which only two short regions (less than 150 bp in all) were comparable with other mycobacteria, apart from Mycobacterium monacense and Mycobacterium gilvum. The PCR restriction analysis pattern of the novel strains also differs from any reported to date. The name Mycobacterium insubricum sp. nov. is proposed for the novel species; the type strain is FI-06250(T) (=DSM 45132(T) =CIP 109609(T)).
International journal of systematic and evolutionary microbiology 07/2009; 59(Pt 6):1518-23. · 2.27 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Mycobacterium arosiense is a newly described species. After noticing it was misidentified as Mycobacterium intracellulare by the commercial identification system GenoType CM (Hein, Nehren, Germany), we detected 4 such strains among 33 that were previously misidentified as M. intracellulare. Three more strains were found among unidentified mycobacteria not tested previously with GenoType. The first case of pulmonary disease due to M. arosiense is reported here, and the novel species, of which so far only one strain had been investigated, is further characterized.
Journal of clinical microbiology 05/2009; 47(6):1947-9. · 4.16 Impact Factor
-
Emerging Infectious Diseases 07/2008; 14(6):984-5. · 6.79 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
The new microbiologica: official journal of the Italian Society for Medical, Odontoiatric, and Clinical Microbiology (SIMMOC) 02/2008; 31(1):151-3. · 1.00 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The characterization of 2 clinical isolates of Mycobacterium sherrisii provides further phenotypic and genotypic information beyond that reported in the article that originally described this species. One of our strains was responsible for pulmonary disease in a middle-aged non-HIV patient; thus, confirming the potential pathogenicity of this species previously reported only in an HIV-positive patient.
Diagnostic Microbiology and Infectious Disease 03/2007; 57(2):221-3. · 2.53 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Four bacterial strains were isolated from independent clinical specimens in different countries and their genotypic and phenotypic characters support their classification in a novel species within the genus Mycobacterium. One strain was clearly responsible for a severe, post-traumatic wound infection in a healthy boy. The novel species, for which the name Mycobacterium monacense sp. nov. is proposed, is yellow-pigmented, non-photochromogenic and grows in less than a week on solid medium. Based on phenotypic investigations alone, distinction of these four strains from known scotochromogenic rapidly growing strains is problematic. However, the novel strains differ from any other mycobacterium in each of the molecular species markers investigated: the 16S rRNA gene, the 16S-23S rRNA gene internal transcribed spacer and the hsp65 gene. Of the strains investigated, two different sequevars were detected for the hsp65 region. Phylogenetic analysis revealed that these four strains were most closely related to Mycobacterium doricum. The type strain of Mycobacterium monacense sp. nov. is B9-21-178T (=DSM 44395T=CIP 109237T).
International journal of systematic and evolutionary microbiology 12/2006; 56(Pt 11):2575-8. · 2.27 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with meropenem, clarithromycin and amikacin.
Journal of Medical Microbiology 02/2006; 55(Pt 1):119-21. · 2.50 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: We report on four cases of infection by the recently described species Mycobacterium parascrofulaceum. In two cases the mycobacterium was isolated from AIDS patients, while in the others it was responsible for pulmonary disease in elderly men. Our findings suggest that M. parascrofulaceum is an opportunistic pathogen, like many other nontuberculous mycobacterial species.
Journal of Clinical Microbiology 09/2005; 43(8):4286-7. · 4.15 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Eight mycobacterial strains isolated during an 11 year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. Phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were Mycobacterium triplex and Mycobacterium lentiflavum. However, the lipids of the cell wall of the test isolates differed from those of the latter species by TLC and presented unique profiles by both GC and HPLC. Genotypic analysis showed that they had unique 16S rRNA gene and internal transcribed spacer (ITS) sequences, and could be differentiated from all other mycobacterial strains by PCR restriction analysis of hsp65. The strains presented high resistance to antimycobacterial drugs. The name Mycobacterium florentinum sp. nov. is proposed for this taxon, with strain FI-93171(T) (=DSM 44852(T) = CIP 108409(T)) as the type strain.
International journal of systematic and evolutionary microbiology 06/2005; 55(Pt 3):1101-6. · 2.27 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A lymph node excision was performed on a 45-year-old woman with left cervical swelling. The disorder which developed after the patient had undergone oral surgery for a severe periodontal disease failed to respond to antimicrobial chemotherapy. A mycobacterial strain subsequently identified by high-performance liquid chromatography analysis of cell wall mycolic acids as Mycobacterium lentiflavum grew from the excised specimen. This case and previously published reports highlight the relevance of M. lentiflavum as an emerging causative agent of mycobacterial cervical lymphadenitis.
Journal of Clinical Microbiology 09/2004; 42(8):3894-7. · 4.15 Impact Factor
-
Enrico Tortoli,
Laura Rindi,
Maria J Garcia,
Patrizia Chiaradonna,
Rosanna Dei,
Carlo Garzelli,
Reiner M Kroppenstedt,
Nicoletta Lari,
Romano Mattei, Alessandro Mariottini,
Gianna Mazzarelli,
Martha I Murcia,
Anna Nanetti,
Paola Piccoli,
Claudio Scarparo
[show abstract]
[hide abstract]
ABSTRACT: The possibility that the strains included within the Mycobacterium avium complex (MAC), but not belonging either to M. avium or to Mycobacterium intracellulare, may be members of undescribed taxa, has already been questioned by several taxonomists. A very homogeneous cluster of 12 strains characterized by identical nucleotide sequences both in the 16S rDNA and in the 16S-23S internal transcribed spacer was investigated. Similar strains, previously reported in the literature, had been assigned either to the species M. intracellulare on the basis of the 16S rDNA similarity or to the group of MAC intermediates. However, several phenotypical and epidemiological characteristics seem to distinguish these strains from all other MAC organisms. The unique mycolic acid pattern obtained by HPLC is striking as it is characterized by two clusters of peaks, instead of the three presented by all other MAC organisms. All of the strains have been isolated from humans and all but one came from the respiratory tract of elderly people. The clinical significance of these strains, ascertained for seven patients, seems to suggest an unusually high virulence. The characteristics of all the strains reported in the literature, genotypically identical to the ones described here, seem to confirm our data, without reports of isolations from animals or the environment or, among humans, from AIDS patients. Therefore, an elevation of the MAC variant was proposed and characterized here, with the name Mycobacterium chimaera sp. nov.; this increases the number of species included in the M. avium complex. The type strain is FI-01069T (=CIP 107892T=DSM 44623T).
International journal of systematic and evolutionary microbiology 08/2004; 54(Pt 4):1277-85. · 2.27 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: INNO-LiPA MYCOBACTERIA (Innogenetics, Ghent, Belgium) is a reverse hybridization DNA probe assay that has been recently improved by increasing the number of identifiable mycobacterial species to 16. Our assessment, performed with 197 mycobacteria belonging to 81 taxa, revealed 100% specificity and sensitivity for 20 out of 23 probes. The probes specific for Mycobacterium fortuitum complex, for the Mycobacterium avium-intracellulare-scrofulaceum group, and for Mycobacterium intracellulare type 2 cross-reacted with several mycobacteria rarely isolated from clinical specimens. The overall sensitivity was 100%, and the overall specificity was 94.4%.
Journal of Clinical Microbiology 10/2003; 41(9):4418-20. · 4.15 Impact Factor
