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Ruolei Xin,
Xiang He,
Hui Xing,
Feng Sun,
Mingjian Ni,
Yuanzhi Zhang,
Zhefeng Meng,
Yi Feng, Shengya Liu,
Jing Wei,
Yiming Shao
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ABSTRACT: To explore the temporal genetic variation of human immunodeficiency virus type 1 CRF07_BC and reconstruct its epidemic in Xinjiang, China, we studied 216 C2-V4 fragments of env genes sampled from 1996 to 2008. Phylogenetic analysis indicates that the viruses prevailing in Xinjiang form a large monophyletic cluster and may have originated from a common ancestor. The epidemic in Xinjiang was probably established around 1995 (95% confidence interval, 1994-1996). We noted an increased diversity of CRF07_BC over time, with a rapid evolutionary rate we estimated to be 8.3x10(-3) substitutions per site per year in the env gene. After 5-6 years of the epidemic (1997-2002), the transmission rate of CRF07_BC in Xinjiang slowed down, although CRF07_BC infection remained at a high prevalence.
Journal of General Virology 04/2009; 90(Pt 7):1757-61. · 3.36 Impact Factor
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ABSTRACT: To investigate more closely the determinants of transmission and escape in HIV-1 internal proteins, we analyzed the characterization of putative N-linked glycosylation sites (PNGSs) and the variable loop of CRF07_BC recombinant human immunodeficiency virus type 1 (HIV-1), isolated from intravenous drug users (IDUs). We studied the characterization of PNGSs and the variable loop in the C1-C5 and V1-V4 regions of the HIV-1 env gene in 12 intravenous drug users (IDUs) who were divided into two groups according to the length of infection time. In addition, two IDUs were longitudinally monitored from the time of seroconversion for 1.5 and 2.5 years. The longitudinal characterization within the individuals on PNGSs and the variable loop in the C1-C5 and V1-V4 region were also observed. Based on the above analysis, we found that PNGSs and the variable loop appeared to increase over time within IDU transmission of CRF07_BC recombinant HIV-1. We argue that limited PNGSs and the length of variable loops may be involved in selective transmission and escape.
AIDS Research and Human Retroviruses 04/2008; 24(3):521-7. · 2.25 Impact Factor
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ABSTRACT: The aim of this study was to investigate the genetic variation of HIV-1 CRF07_BC, the most prevalent circulating strain in intravenous drug users (IDUs) in China. We studied the diversity in the C2-V5 region of the HIV-1 env gene and in the p17-p24 region of the HIV-1 gag gene from the same samples in 12 IDUs who were divided into two groups according to the length of infection time. Two IDUs were longitudinally monitored from the time of seroconversion for 2-2.5 years. The viral divergence from the founder strain and the viral population diversity between sequential time points were analyzed in two men. The data show that the divergence of the env gene is higher than that of gag in general, while the diversity of the gag gene is sometimes higher than that of env during the course of HIV evolution. In addition, env and gag gene diversity increased over time. The observed patterns and associations may enhance our understanding of HIV-1 evolution.
Archives of Virology 02/2008; 153(7):1233-40. · 2.11 Impact Factor
