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ABSTRACT: The influence of the activation of capsaicin-sensitive nerves with capsaicin on the oxidative modification of blood plasma proteins and arterial blood pressure was studied in Wistar rats fed with 12.5% fructose in drinking water for 10 weeks. The obtained results indicate that fructose feeding induces an increase in the arterial blood pressure and the content of plasma blood protein carbonyl derivates. At the same time, in hypertensive rats, the stimulation of sensory nerves by capsaicin (1 mg/kg, i.p.) decreases the content of oxidized proteins in the plasma and normalizes the arterial blood pressure. It is suggested that capsaicin-sensitive nerves are involved in the regulation of oxidative destruction of proteins as well as in blood pressure control under metabolic disturbances produced by prolonged fructose feeding.
Eksperimental'naia i klinicheskaia farmakologiia 01/2012; 75(3):3-6.
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ABSTRACT: Oxidative modification of blood proteins in rats after lesioning of afferent nerves with capsaicin (150 mg/kg, s.c.) and changes in nitric oxide (NO) contents were studied in terms of levels of protein carbonyl derivatives. Deafferentation of capsaicin-sensitive nerves induced increases in the level of protein carbonyl derivatives. Increases in NO levels using L-arginine did not alter measures of the oxidative modification of proteins after lesioning of capsaicin-sensitive innervation. A similar lack of effect was seen after blockade of neuronal NO synthase (n-NOS) by the selective inhibitor 7-nitroindazole (7-NI). Increases in markers of oxidative destruction of proteins in capsaicin-treated animals were seen after blockade of NO synthesis by the nonselective inhibitor L-NAME. These results show that deafferentation of capsaicin-sensitive nerves induces oxidative modification of proteins and that this process involves NO.
Neuroscience and Behavioral Physiology 06/2011; 41(5):479-83.
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ABSTRACT: Regulation of activity of serine proteinase inhibitor a1-antitrypsin and nonspecific proteinase inhibitor alpha(2)-macroglobulin in the blood by nitric oxide was studied in intact rats and animals with damage to capsaicin-sensitive nerves. Nonselective nitric oxide synthase inhibitor L-NAME produced a dose-dependent increase in alpha(1)-antitrypsin activity in intact animals. Neuronal NO synthase inhibitor 7-nitroindazole increased alpha(2)-macroglobulin activity. Deafferentation with capsaicin was followed by a decrease in alpha(1)-antitrypsin activity. Both inhibitors of nitric oxide synthase increased activity of alpha(1)-antitrypsin in capsaicin-receiving rats. Nitric oxide precursor L-arginine had a normalizing effect on reduced activity of alpha(1)-antitrypsin after capsaicin deafferentation. Our results suggest that nitric oxide has a modulatory effect on activity of proteinase inhibitors and is involved in the effector influence of capsaicin-sensitive nerves on alpha(1)-antitrypsin activity.
Bulletin of Experimental Biology and Medicine 10/2008; 146(3):375-8. · 0.27 Impact Factor
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ABSTRACT: Effects of functional ablation of peptidergic sensory nerves with neurotoxic doses of capsaicin (150 mg/kg, s/c) as well as of their stimulation with small doses of capsaicin (5 mg/kg, i/p) on activity of proteinase inhibitors: alpha1-antitrypsin (alpha1-AT)-serine proteinase inhibitor and alpha2-macroglobulin (alpha2-MG)-nonspecific inhibitor were investigated in rat blood. The present results indicate alternative changes in activity of these proteinase inhibitors after damage of capsaicin-sensitive nerves: increasing decline in activity of alpha1-AT 1 and 3 or 14 days after administration of capsaicin and increase in activity of alpha2-MG land 3 day after the injection. The stimulation of afferent nerves with capsaicin did not change activity of the proteinase inhibitors 1 and 24 hours after the injection. It is suggested that the stable decrease in activity of alpha1-AT during a long period after the damage of capsaicin-sensitive nerves indicates an important role for these nerves in the regulating alpha1-AT that may exert a tonic effect on the activity alpha1-AT.
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 10/2006; 92(9):1078-84.
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Z S Tolochko
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ABSTRACT: Electrical stimulation of the trigeminal caudal nucleus in rats evoked increases in the permeability of eye microvessels. The microvascular effect did not appear when the nucleus was stimulated after administration of capsaicin 10 days before surgery (total dose 150 mg/kg, s.c., on two sequential days, given as 20, 30, 50, and 50 mg/kg). It is suggested that capsaicin-sensitive neurons in the trigeminal ganglion mediate the microvascular effect of stimulation of the trigeminal caudal nucleus.
Neuroscience and Behavioral Physiology 07/2003; 33(5):435-8.
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ABSTRACT: Serpin alpha-1-proteinase inhibitor have been studied in human subjects and in mice of different lines as acute phase reactant and during tumor development. In humans, there was no difference of serpin activity between men and women. Increased activity was noted in men with acute trauma (acute phase reaction). Comparatively to male, in female mice of different lines decreased activity of serum alpha-1-proteinase inhibitor, was shown. There was no increase of alpha-1-proteinase inhibitor activity during inflammation induced by zymosan administration in mice. alpha-1-proteinase inhibitor belongs to acute phase reactants in humans but not in mice; for mice alpha-2-macroglobulin is a more typical acute phase reactant as compared to alpha-1-proteinase inhibitor. Murine tumor development (hepatoma HA-1, lymphosarcoma LS, Lewis lung adenocarcinoma) was followed by a decreased activity of serum alpha-1-proteinase inhibitor both in successfully treated and untreated groups. According to data of literature, similar dated were obtained in humans with tumors. It was suggested that changes of expressiln of alpha-1-proteinase inhibitor by tumors and its secretion were involved in decreased activity of alpha-1-proteinase inhibitor.
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 05/2003; 89(4):420-6.
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Z S Tolochko
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ABSTRACT: Electrical stimulation of the trigeminal caudate nucleus increased permeability of the eye blood microvessels in rats. Ten days after capsaicin pre-treatment (total dose 150 mg/kg, s/c for 2 days: 20, 30, 50, 50 mg/kg) the electrical stimulation did not evoke any changes of the microvascular permeability in the eye. The data suggest that the effect of the stimulation is mediated through capsaicin-sensitive neurones of the trigeminal ganglion.
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 02/2002; 88(1):8-12.
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Z S Tolochko
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ABSTRACT: An increase in vascular permeability as measured by the Evans Blue dye leakage into forepaws, was observed 1 hour following the destruction of mamillo-tegmental tracts in rats. Ten days after capsaicin pre-treatment the destruction did not induce any changes of the microvascular permeability in the skin forepaws. The findings suggest that the descending projections of hypothalamic mamillary nuclei might regulate the mechanisms of the skin microvessel permeability, the action being mediated through capsaicinsensitive neurons of the spinal ganglion.
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 02/2001; 87(1):137-41.
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ABSTRACT: Electrocoagulation in the region of mamillary complex of the hypothalamus induced disturbances in vascular permeability in
the rat limb skin. This effect was prevented by pretreatment of the animals with the neurotoxin capsaicin, which indicates
participation in the effect of the peptidergic capsaicin-sensitive neurons. The data are discussed in relation to possible
mechanisms of central modulation of efferent function of the primary sensory neurons.
Bulletin of Experimental Biology and Medicine 11/1997; 124(6):1241-1243. · 0.27 Impact Factor
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ABSTRACT: Contralateral increased exudation of plasma proteins, as measured by Evans Blue leakage into the fore paws, was produced by unilateral electrocoagulation of the mamillary nuclei in rats. Ten days after adult capsaicin pretreatment (increasing doses/4 days total 200 mg/kg, s.c.) the unilateral destruction of these nuclei did not evoke any changes of skin blood vessel permeability in both contralateral and unilateral paws. The findings show that the central nervous system may modulate the capsaicin-sensitive peptidergic neuron action on blood vessel permeability.
Brain Research 04/1997; 752(1-2):324-6. · 2.73 Impact Factor
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ABSTRACT: In early and late stages after different types of deafferentation of rat cornea extents of leukocyte infiltration and pathological changes are variable. Change of thickness of anterior epithelium and substantia propria, stratification of substantia propria fibres as well as epitheliocyte death and erosion occur only at late time intervals of deafferentation (after 1 d) when a large number of leukocytes appear in cornea. One day after the deafferentation performed following preliminary leukopoiesis suppression by irradiation, single leukocytes are encountered only in substantia propria of cornea while anterior epithelium thickness does not differ from that of intact animals, and the extent of pathological changes in substantia propria is reduced. These data demonstrate polymorphonuclear leukocyte participation in the development of neurogenic dystrophia in cornea.
Morfologiia (Saint Petersburg, Russia) 02/1997; 112(5):39-42.
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ABSTRACT: Electrical stimulation of the hypothalamic mamillary nuclei increased the permeability of the skin blood vessels in rats. Pretreatment of the animals with capsaicin prevented the effect. The mamillary nuclei seem to take part in the central mechanisms of efferent function of the capsaicin-sensitive neurons.
Fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 01/1997; 82(12):1-5.
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ABSTRACT: The results of the present study, in the rat and cat, indicate that not only a lesion of peripheral nerve or capsaicin pretreatment but also pharmacological deafferentation with local anaesthetic or disruption of the connections between primary sensory neurons and the central nervous system are effective in producing dystrophic changes in tissues. These effects of deafferentation do not seem to depend on the sympathetic or parasympathetic efferents. Dystrophic changes are connected with microcirculation disturbances: slow down of local blood flow, elevation of the vascular permeability, oedema and leucocyte infiltration. The findings indicate that capsaicin-sensitive primary sensory neurons are the afferent part of some reflex arrangement which participates in the regulation of microcirculation and the maintenance of trophic processes in peripheral tissues. The efferent part of this arrangement is unknown.
Neuroscience 02/1991; 42(2):555-60. · 3.38 Impact Factor
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ABSTRACT: The destruction of rat hypothalamic mamillary nuclei causes impaired permeability of eye microvessels receiving afferent innervation from the trigeminal ganglion. This effect was eliminated by pre-treatment of the animals with capsaicin (200 mg/kg), which exhausted the content of substance P and calcitonin-gene-specific peptide, from the primary capsaicin-sensitive neurons of trigeminal and spinal ganglia. The findings show that the peripheral effect caused by the destruction of mamillary nuclei is mediated through capsaicin-sensitive neurons of the trigeminal ganglion.
Patologicheskaia fiziologiia i eksperimental'naia terapiia
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ABSTRACT: Systemic administration of capsaicin (a neurotoxin of afferent nerves) in a dose of 150 mg/kg led to a decrease in the activity of alpha 1-antitrypsin (alpha 1-AT) in the blood of male Wistar rats. This effect is related to impairment of the local effector function of capsaicin-sensitive nerves in the liver, which is the main source of alpha 1-AT in the blood. Analogous results were obtained upon cutting of the hepatic branch of the vagus nerve involving capsaicin-sensitive afferent fibers of the liver.
Eksperimental'naia i klinicheskaia farmakologiia 67(2):67-9.
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ABSTRACT: Stimulation of the mamillary nuclei of the rat hypothalamus induced increases in the permeability of forelimb skin microvessels. This effect was not seen in rats stimulated after administration of capsaicin at a dose (150 mg/kg) sufficient to deplete vasoactive neuropeptides from the peripheral nerve terminals of capsaicin-sensitive neurons. These data indicate a role for the mamillary nuclei in central mechanisms modulating the effector functions of primary capsaicin-sensitive neurons.
Neuroscience and Behavioral Physiology 28(2):201-5.
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ABSTRACT: The results of the present study, in the rat and cat, indicate that not only a lesion of peripheral nerve or capsaicin pretreatment but also pharmacological deafferentation with local anaesthetic or disruption of the connections between primary sensory neurons and the central nervous system are effective in producing dystrophic changes in tissues. These effects of deafferentation do not seem to depend on the sympathetic or parasympathetic efferents. Dystrophic changes are connected with microcirculation disturbances: slow down of local blood flow, elevation of the vascular permeability, oedema and leucocyte infiltration.The findings indicate that capsaicin-sensitive primary sensory neurons are the afferent part of some reflex arrangement which participates in the regulation of microcirculation and the maintenance of trophic processes in peripheral tissues. The efferent part of this arrangement is unknown.
Neuroscience.
