Kazimierz Wrobel

Universidad de Guanajuato, Ciudad Guanajuato, Guanajuato, Mexico

Are you Kazimierz Wrobel?

Claim your profile

Publications (78)203.95 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Three α-ketoaldehydes, potentially present in high fructose agave syrups (HFASs) as intermediates of the Maillard reaction, were determined. A previously reported HPLC-FLD procedure based on pre-column derivatisation with 4-methoxy-o-phenylenediamine was adopted, yielding the method quantification limits 0.11mg/kg, 0.10mg/kg, 0.09mg/kg for glyoxal, methylglyoxal (MGo) and diacetyl, respectively. The obtained results revealed high concentrations of methylglyoxal in HFASs (average 102±91mg/kg, range 15.6-315mg/kg) as compared to commercial Mexican bee honeys or corn syrups. Hydrogen peroxide was generated in all HFASs upon dilution, yet to less extent than in bee honeys. HFASs presented bacteriostatic activity against Bacillus subtilis and Escherichia coli; catalase addition had minimum effect on the assay results in syrups with elevated MGo. Principal component analysis revealed direct association between growth inhibition and MGo. It is concluded that elevated concentration of MGo in HFASs is at least in part responsible for their non-peroxide bacteriostatic activity.
    Food chemistry. 12/2014; 165C:444-450.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Experimental evidence indicates that diabetic patients and individuals with impaired copper homeostasis could be at risk of molybdenum toxicity. A self-administered food frequency questionnaire revealed that in central Mexico, diabetic patients with severe complications tend to consume beans more often than individuals with less advanced disease. Four varieties of Phaseolus vulgaris were comparatively evaluated as the dietary sources of two elements; the results showed molybdenum concentration decreasing in the order peruvian > pinto > mayflower > black, whereas for copper, the order was peruvian > pinto ∼ black > mayflower. The two elements were determined in pre-soaking water, cooked legumes, and broth obtained in cooking procedure; an in vitro gut model was also applied to assess potentially bioavailable fraction of both elements in cooked beans. The results indicated that the black variety would be the healthiest bean choice for diabetic patients and individuals susceptible to Mo toxicity. Relatively low total molybdenum was found in this variety (2.9 ± 1.4 versus 4.3-10.9 μg g(-1) in other types), element availability was also low (15 % in supernatant from enzymolysis, 24.9 % in combined broth + supernatant fractions), and the molar ratio of Cu/Mo was the highest among four types (41, versus Cu/Mo <10 in peruvian, pinto, or mayflower). Considering peruvian and pinto beans, broth elimination would help to lower molybdenum intake with marginal effect on Cu/Mo molar ratio. This recommendation would be especially important for peruvian variety, which provided 1090, 803, and 197 μg day(-1) of molybdenum in raw grains, broth + supernatant, and supernatant, respectively (based on 100-g portion), exceeding the recommended daily allowance of 45 μg day(-1).
    Biological trace element research. 11/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The ability of human serum albumin to capture unbound copper under different clinical conditions is an important variable potentially affecting homeostasis of this element. Here, we propose a simple procedure based on size-exclusion chromatography with on-line UV and nitrogen microwave-plasma atomic-emission spectrometry (MP-AES) for quantitative evaluation of Cu(II) binding to HSA upon its glycation in vitro. The Cu-to-protein molar ratio for non-glycated albumin was 0.98 ± 0.09; for HSA modified with glyoxal (GO), methylglyoxal (MGO), oxoacetic acid (GA), and glucose (Glc), the ratios were 1.30 ± 0.22, 0.72 ± 0.14, 0.50 ± 0.06, and 0.95 ± 0.12, respectively. The results were confirmed by using ICP-MS as an alternative detection system. A reduced ability of glycated protein to coordinate Cu(II) was associated with alteration of the N-terminal metal-binding site during incubation with MGO and GA. In contrast, glycation with GO seemed to generate new binding sites as a result of tertiary structural changes in HSA. Capillary reversed-phase liquid chromatography with electrospray-ionization quadrupole-time-of-flight tandem mass spectrometry enabled detection and identification of Cu(II) coordinated to the N-terminal metal-binding site (Cu(II)-DAHK) in all tryptic digests analyzed. This is the first report confirming Cu(II)-DAHK species in HSA by means of high-resolution tandem mass spectrometry, and the first report on the use of MP-AES in combination with chromatographic separation.
    Analytical and Bioanalytical Chemistry 11/2014; · 3.66 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this study was to investigate the effects of cadmium [Cd(II) as cadmium chloride], selenium [Se(IV) as sodium selenite] and their mixtures on phenolic compounds (PCs) and antioxidant activity (AOx) in Lepidium sativum. The biomass fractions corresponding to free (F1), soluble glycoside-bound (F2) and cell wall ester-bound phenolics (F3) were obtained for each treatment and PCs were screened by gas chromatography (GC-FID); F1 and F2 fractions were also analyzed by liquid chromatography with UV and fluorimetric detection. The treated plants presented different profiles of PCs as compared to controls, specifically in F1 and F2 fractions; the plant response was element-, and concentration-dependent. The cultures challenged with Cd(II) up to 5 mg L−1, presented higher AOx with respect to controls, and this increase was associated with glycoside-bound PCs, whereas for Se(IV) the increase of AOx was less marked and associated with free PCs. Under simultaneous exposure to Cd(II) + Se(IV) (0.5–2.0 mg L−1 each), the AOx values were relatively constant and lower than those found in the presence of Se(IV) or Cd(II) alone, providing further evidence of the protective role of Se(IV) against stress imposed by Cd(II) in this plant species. The evaluation of AOx of individual PCs and the results of principal component analysis enabled to attain several relationships among exposure conditions, antioxidant activity, free- and glycoside-bound phenolic compounds.
    Acta Physiologiae Plantarum 01/2014; 35(2). · 1.31 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Bioanalytical relevance of glyoxal (Go) and methylglyoxal (MGo) arises from their role as biomarkers of glycation processes and oxidative stress. The third compound of interest in this work is diacetyl (DMGo), a component of different food products and alcoholic beverages and one of the small α-ketoaldehydes previously reported in urine. The original idea for the determination of the above compounds by reversed phase high-performance liquid chromatography (HPLC) with fluorimetric detection was to use 4-methoxy-o-phenylenediamine (4MPD) as a derivatizing reagent and diethylglyoxal (DEGo) as internal standard. Acetonitrile was added to urine for matrix precipitation, and derivatization reaction was carried out in the diluted supernatant at neutral pH (40 °C, 4 h); after acidification, salt-induced phase separation enabled recovery of the obtained quinoxalines in the acetonitrile layer. The separation was achieved within 12 min using a C18 Kinetex column and gradient elution. The calibration detection limits for Go, MGo, and DMGo were 0.46, 0.39, and 0.28 μg/L, respectively. Within-day precision for real-world samples did not exceed 6%. Several urine samples from healthy volunteers, diabetic subjects, and juvenile swimmers were analyzed. The sensitivity of the procedure proposed here enabled detection of differences between analyte concentrations in urine from patients at different clinical or exposure-related conditions.
    Analytical Biochemistry 01/2014; 449:52–58. · 2.58 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Bioanalytical relevance of glyoxal (Go) and methylglyoxal (MGo) arises from their role as biomarkers of glycation processes and oxidative stress. The third compound of interest in this work is diacetyl (DMGo), a component of different food products and alcoholic beverages and one of the small α-ketoaldehydes previously reported in urine. The original idea for the determination of above compounds by reversed phase HPLC with fluorimetric detection was to use 4- methoxy-o-phenylenediamine (4MPD) as a derivatizing reagent and diethylglyoxal (DEGo) as an internal standard. Acetonitrile was added to urine for matrix precipitation and derivatization reaction was carried out in the diluted supernatant at neutral pH (40 °C, 4h); after acidification, salt-induced phase separation enabled for recovery of the obtained quinoxalines in acetonitrile layer. The separation was achieved within 12 min, using C18 Kinetex column and gradient elution. The calibration detection limits for Go, MGo, DMGo were 0.46 μg/l, 0.39 μg/l and 0.28 μg/l, respectively. Within-day precision for real world samples did not exceed 6%. Several urine samples from healthy volunteers, diabetic subjects and juvenile swimmers were analyzed. The sensitivity of proposed here procedure enabled for detection of differences between analyte concentrations in urines from patients at different clinical or exposure conditions.
    Analytical Biochemistry 12/2013; · 2.58 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Arsenic release from the abandoned mines and its fate in a local stream were studied. Physicochemical parameters, metals/metalloids and arsenic species were determined. One of the mine drainages was found as a point source of contamination with 309 μg L(-1) of dissolved arsenic; this concentration declined rapidly to 10.5 μg L(-1) about 2 km downstream. Data analysis confirmed that oxidation of As(III) released from the primary sulfide minerals was favored by the increase of pH and oxidation reduction potential; the results obtained in multivariate approach indicated that self-purification of water was due to association of As(V) with secondary solid phase containing Fe, Mn, Ca.
    Bulletin of Environmental Contamination and Toxicology 09/2013; · 1.11 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In this work, the effect of cadmium (0-5.0 mg L(-1) as cadmium chloride, Cd(ii)) and selenium (0-2.0 mg L(-1) as sodium selenite, Se(iv)) was studied in Lepidium sativum with specific focus on glyoxal (GO) and methylglyoxal (MGO) and on the cellular distribution of both elements under different exposure conditions. The concentrations of two reactive α-ketoaldehydes present as natural metabolites and as by-products of lipid peroxidation, were increased in plants treated with Cd(ii), providng complementary experimental evidence on element phytotoxicity in garden cress, in terms of oxidative damage. Even though for higher than 1.0 mg L(-1) Se in medium similar adverse effect was found, under simultaneous exposure to both elements the changes in GO and MGO concentrations were clearly attenuated as compared to a single stressor treatment. This effect was accompanied by lower uptake of the two elements, significant decrease of their relative distribution in the fraction containing polar compounds and their increase in fraction corresponding to insoluble cell fragments/components, suggesting that the direct in vivo interaction between two element forms might be involved in the favorable effects of simultaneous treatment with Cd(ii) + Se(iv). The fluorescence spectra obtained for biomass extracts corresponding to different exposure conditions suggested possible in vivo formation of CdSe quantum dots; however further studies are needed for ultimate identification and characterization of such nanoparticulate species.
    Metallomics 06/2013; · 4.10 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Large amounts of phosphate fertilizers utilized in agriculture and their relatively poor efficiency are of high ecological and economic concern. Therefore, transgenic plants capable of metabolizing phosphite are being engineered. In support of this biotechnological task, analytical speciation of phosphorus in biological tissues is required. In this study, plant extracts were analyzed by liquid chromatography - inductively coupled plasma mass spectrometry at m/z of elemental phosphorus and phosphorus oxide ions. Using polymeric-based anion exchange column and millimolar concentration of nitric acid in potassium phthalate mobile phase (pH 2.5), phosphite and phosphate ions were baseline resolved with retention times 6.95 ± 0.03 min and 7.90 ± 0.03 min and with total chromatographic run time 10 min. The detection limits were 1.58 μgP L-1 and 1.74 μgP L-1 at m/z 47, as compared to 2.18 μgP L-1 and 2.04 μgP L-1 at m/z 31, respectively. The results obtained in real world samples for the two detection modes were in good agreement, yet signal acquisition at m/z 47 enabled for better precision without collision/reaction cell (RSD below 2%) as compared to RSD around 4% obtained at m/z 31 while using He pressurized cell (3.5 mL min-1).
    Journal of Agricultural and Food Chemistry 06/2013; · 3.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In the present work, application of the previously established reversed-phase liquid chromatography procedure based on fluorescent labeling of cytosine and methylcytosine moieties with 2-bromoacetophenone (HPLC-FLD) is presented for simultaneous evaluation of global DNA and total RNA methylation at cytosine carbon 5. The need for such analysis was comprehended from the recent advances in the field of epigenetics that highlight the importance of non-coding RNAs in DNA methylation and suggest that RNA methylation might play a similar role in the modulation of genetic information, as previously demonstrated for DNA. In order to adopt HPLC-FLD procedure for DNA and RNA methylation analysis in a single biomass extract, two extraction procedures with different selectivity toward nucleic acids were examined, and a simplified calibration was designed allowing for evaluation of methylation percentage based on the ratio of chromatographic peak areas: cytidine/5-methylcytidine for RNA and 2´-deoxycytidine/5-methyl-2'-deoxycytidine for DNA. As a proof of concept, global DNA and total RNA methylation were determined in Lepidium sativum hydroponically grown in the presence of different Cd(II) or Se(IV) concentrations, expecting that plant exposure to abiotic stress might affect not only global DNA but also total RNA methylation. The results obtained showed the increase of DNA methylation in the treated plants up to concentration levels 2 mg L(-1) Cd and 1 mg L(-1) Se in the growth medium. For higher stressors' concentration, global DNA methylation tended to decrease. Most importantly, an inverse correlation was found between DNA and RNA methylation levels (r = -0.6788, p = 0.031), calling for further studies of this particular modification of nucleic acids in epigenetic context.
    Analytical and Bioanalytical Chemistry 01/2013; · 3.66 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The augmented consumption of dietary advanced glycation end products (dAGEs) has been associated with increased oxidative stress and inflammation, however, there is insufficient information over the effect on insulin resistance. The objective of the present study is to investigate the effect of dAGEs restriction on tumor necrosis factor-α (TNF-α), malondialdehyde, C-reactive protein (CRP), and insulin resistance in DM2 patients. We carried out a randomized 6 weeks prospective study in two groups of patients: subjects with a standard diet (n = 13), vs low dAGEs (n = 13). At the beginning and the end of study, we collected anthropometric measurements, and values of circulating glucose, HbA1c, lipids, insulin, serum AGEs, CRP, TNF-α and malondialdehyde. Anthropometric measurements, glucose, and lipids were similar in both groups at base line and at the end of the study. Estimation of basal dAGEs was similar in both groups; after 6 weeks it was unchanged in the standard group but in the low dAGEs group decreased by 44% (p<0.0002). Changes in TNF-α levels were different under standard diet (12.5 ± 14.7) as compared with low dAGEs (-18.36 ± 17.1, p<0.00001); changes in malondialdehyde were different in the respective groups (2.0 ± 2.61 and -0.83 ± 2.0, p<0.005) no changes were found for insulin levels or HOMA-IR. In conclusion, The dAGEs restriction decreased significantly TNF-α and malondialdehyde levels.
    Journal of Clinical Biochemistry and Nutrition 01/2013; 52(1):22-6. · 2.25 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The purpose of this work was to examine the feasibility of using municipal sewage sludge from San Miguel de Allende city WWTP, located at central Mexico, for compost production. Experimental piles were prepared with an aerobic digested activated sludge from a municipal WWTP dewatered in a filter press, grass was added as a nitrogen source and bulking was increased by mixing with either crushed porous volcanic material called tezontle (SGT) or wooden sticks wastes collected from municipal gardening of green areas (SGW). The specific composition of the compost piles was as follows: 30 % municipal sewage sludge, 60 % grass clippings and 10 % small wooden sticks (compost SGW); 30 % municipal sewage sludge, 60 % grass clippings and 10 % tezontle (compost SGT); 100 % sewage sludge (control). The efficient reduction of fecal bacteria was achieved in both piles yielding the USEPA class A compost, yet better process performance was obtained in SGW. In this pile the thermophilic phase lasted seven days longer and higher temperatures were reached, for which the desired removal of coliforms was achieved at two weeks of composting versus three weeks required in SGT. The analysis of typical spectrophotometric assays used in the compost characterization, confirmed the decomposition of organic matter and generation of humic substances. The fractionation of humic extracts by size exclusion chromatography with spectrophotometric detection indicated the increase of humic substances amount, and also the increase of molecular mass with the time of composting process. Overall, this work demonstrates the feasibility of municipal sewage sludge compost production for soil fertilization and provides new data about the changes of the molecular mass in humic fraction during composting.
    Revista Internacional de Contaminacion Ambiental 01/2013; 29(Sup 3):89-97. · 0.17 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Plant cells have the capacity to generate a new plant without egg fertilization by a process known as somatic embryogenesis (SE), in which differentiated somatic cells can form somatic embryos able to generate a functional plant. Although there have been advances in understanding the genetic basis of SE, the epigenetic mechanism that regulates this process is still unknown. Here, we show that the embryogenic development of Coffea canephora proceeds through a crosstalk between DNA methylation and histone modifications during the earliest embryogenic stages of SE. We found that low levels of DNA methylation, histone H3 lysine 9 dimethylation (H3K9me2) and H3K27me3 change according to embryo development. Moreover, the expression of LEAFY COTYLEDON1 (LEC1) and BABY BOOM1 (BBM1) are only observed after SE induction, whereas WUSCHEL-RELATED HOMEOBOX4 (WOX4) decreases its expression during embryo maturation. Using a pharmacological approach, it was found that 5-Azacytidine strongly inhibits the embryogenic response by decreasing both DNA methylation and gene expression of LEC1 and BBM1. Therefore, in order to know whether these genes were epigenetically regulated, we used Chromatin Immunoprecipitation (ChIP) assays. It was found that WOX4 is regulated by the repressive mark H3K9me2, while LEC1 and BBM1 are epigenetically regulated by H3K27me3. We conclude that epigenetic regulation plays an important role during somatic embryogenic development, and a molecular mechanism for SE is proposed.
    PLoS ONE 01/2013; 8(8):e72160. · 3.53 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND: The micropropagation is a powerful tool to scale up plants of economical and agronomical importance, enhancing crop productivity. However, a small but growing body of evidence suggests that epigenetic mechanisms, such as DNA methylation and histone modifications, can be affected under the in vitro conditions characteristic of micropropagation. Here, we tested whether the adaptation to different in vitro systems (Magenta boxes and Bioreactors) modified epigenetically different clones of Agave fourcroydes and A. angustifolia. Furthermore, we assessed whether these epigenetic changes affect the regulatory expression of KNOTTED1-like HOMEOBOX (KNOX) transcription factors. RESULTS: To gain a better understanding of epigenetic changes during in vitro and ex vitro conditions in Agave fourcroydes and A. angustifolia, we analyzed global DNA methylation, as well as different histone modification marks, in two different systems: semisolid in Magenta boxes (M) and temporary immersion in modular Bioreactors (B). No significant difference was found in DNA methylation in A. fourcroydes grown in either M or B. However, when A. fourcroydes was compared with A. angustifolia, there was a two-fold difference in DNA methylation between the species, independent of the in vitro system used. Furthermore, we detected an absence or a low amount of the repressive mark H3K9me2 in ex vitro conditions in plants that were cultured earlier either in M or B. Moreover, the expression of AtqKNOX1 and AtqKNOX2, on A. fourcroydes and A. angustifolia clones, is affected during in vitro conditions. Therefore, we used Chromatin ImmunoPrecipitation (ChIP) to know whether these genes were epigenetically regulated. In the case of AtqKNOX1, the H3K4me3 and H3K9me2 were affected during in vitro conditions in comparison with AtqKNOX2. CONCLUSIONS: Agave clones plants with higher DNA methylation during in vitro conditions were better adapted to ex vitro conditions. In addition, A. fourcroydes and A. angustifolia clones displayed differential expression of the KNOX1 gene during in vitro conditions, which is epigenetically regulated by the H3K4me3 and H3K9me2 marks. The finding of an epigenetic regulation in key developmental genes will make it important in future studies to identify factors that help to find climate-resistant micropropagated plants.
    BMC Plant Biology 11/2012; 12(1):203. · 4.35 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this work was to examine possible effects that the exposure to cadmium and selenium might have in Lepidium sativum. Nine hydroponic cultures were obtained, according to the three level factorial design that covered plants exposure to 0, 0.5, 2.0 mg L-1 of cadmium (as cadmium chloride) and selenium (as sodium selenite). Shoots growth was evaluated during two weeks after germination and the following parameters were measured in the biomass: Cd, Se, Mn, Zn, Cu, Mo, fatty acids and malondialdehyde. The results obtained indicate that selenium is capable to counterbalance the adverse effects of cadmium in terms of growth inhibition, decreased concentration levels of essential micronutrients and oxidative damage; however, such protective role is apparently restricted by the concentration levels of two elements in the growth medium.
    Journal of the Mexican Chemical Society 01/2012; 56(1):3-9. · 0.28 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We previously showed that a VLDL- and LDL-rich mix of human native lipoproteins induces a set of repressive epigenetic marks, i.e. de novo DNA methylation, histone 4 hypoacetylation and histone 4 lysine 20 (H4K20) hypermethylation in THP-1 macrophages. Here, we: 1) ask what gene expression changes accompany these epigenetic responses; 2) test the involvement of candidate factors mediating the latter. We exploited genome expression arrays to identify target genes for lipoprotein-induced silencing, in addition to RNAi and expression studies to test the involvement of candidate mediating factors. The study was conducted in human THP-1 macrophages. Native lipoprotein-induced de novo DNA methylation was associated with a general repression of various critical genes for macrophage function, including pro-inflammatory genes. Lipoproteins showed differential effects on epigenetic marks, as de novo DNA methylation was induced by VLDL and to a lesser extent by LDL, but not by HDL, and VLDL induced H4K20 hypermethylation, while HDL caused H4 deacetylation. The analysis of candidate factors mediating VLDL-induced DNA hypermethylation revealed that this response was: 1) surprisingly, mediated exclusively by the canonical maintenance DNA methyltransferase DNMT1, and 2) independent of the Dicer/micro-RNA pathway. Our work provides novel insights into epigenetic gene regulation by native lipoproteins. Furthermore, we provide an example of DNMT1 acting as a de novo DNA methyltransferase independently of canonical de novo enzymes, and show proof of principle that de novo DNA methylation can occur independently of a functional Dicer/micro-RNA pathway in mammals.
    BMC Genomics 11/2011; 12:582. · 4.40 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In eukaryotes, actual DNA methylation patterns provide biologically important information, for which both, genome-wide and locus-specific methylation at cytosine residues have been extensively studied. The original contribution of this work relies on the selective derivatization of cytosine moieties with 2-bromoacetophenone for the determination of global DNA methylation by reversed phase high performance liquid chromatography with spectrofluorimetric detection. The important features of the proposed procedure are as follows: (1) no need for the elimination of RNA, (2) detection limits for cytidine, 2'-deoxycytidine, 5-methylcytidine, and 5-methyl-2'-deoxycytidine in the range of 14.4-22.7 fmol, (3) feasibility for the detection of 0.06% of methylation in a low amount of DNA (80 ng), (4) potential viability for the evaluation of RNA methylation, and (5) relative simplicity in terms of analytical instrumentation and personnel training. The results obtained in the analysis of salmon testes DNA and nucleic acids from plant, human blood, and earthworms demonstrate the utility of the proposed procedure in biological studies and, in particular, for evaluation of the potential effect of environmental factors on actual DNA methylation in different types of living organisms.
    Analytical Chemistry 09/2011; 83(20):7999-8005. · 5.82 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In this work, possible relationships between global DNA methylation and metal/metalloid concentrations in earthworms have been explored. Direct correlation was observed between soil and tissue As, Se, Sb, Zn, Cu, Mn, Ag, Co, Hg, Pb (p< 0.05). Speciation results obtained for As and Hg hint at the capability of earthworms for conversion of inorganic element forms present in soil to methylated species. Inverse correlation was observed between the percentage of methylated DNA cytosines and total tissue As, As + Hg, As + Hg + Se + Sb (β = -0.8456, p = 0.071; β = -0.9406, p = 0.017; β = -0.9526, p = 0.012 respectively), as well as inorganic As + Hg (β = -0.8807, p = 0.049). It was concluded that earthworms would be particularly helpful as bioindicators of elements undergoing in vivo methylation and might also be used to assess the related risk of epigenetic changes in DNA methylation.
    Environmental Pollution 07/2011; 159(10):2387-92. · 3.73 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: An alcohol dehydrogenase gene, adh1, has been identified in the vascular wilt fungus Fusarium oxysporum f. sp. lycopersici. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that adh1 is highly expressed in mycelia grown in potato dextrose liquid medium (PDB) under hypoxic conditions, as compared to mycelia grown under aerobic conditions. One spontaneous allyl alcohol-resistant (Ally(R)) mutant exhibited insertion of an incomplete F.oxysporum transposable element, while another mutant contained a short (13 nucleotide) deletion, in both cases interrupting the coding region of the adh1 gene. These mutations caused deficiency in Adh activity due to loss of the main constitutive isoform of Adh1, as well as alteration of different physiological parameters related to carbon and energy metabolism, including the ability to use ethanol as a carbon source under aerobic conditions; impaired growth under hypoxic conditions with glucose as the carbon source; and diminished production of ethanol in glucose-containing medium. Interestingly, the adh1 mutations resulted in a significant delay in fungal disease development in tomato plants. Complementation with the wild-type adh1 allele repaired all defects caused by mutation, indicating that the product of the adh1 gene has dual enzymatic functions (fermentative and oxidative), depending on culture conditions, and is also required for full fungal virulence.
    Fungal Genetics and Biology 06/2011; 48(9):886-95. · 3.26 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study describes pentachlophenol (PCP) sorption in nylon fiber in which Rhizopus oryzae ENHE was immobilized to remove the chemical compound. The experimental sorption data were analyzed using the Langmuir, Freundlich, and Redlich-Peterson isotherm models using non-linear error functions to fit the experimental data to the three models. Results showed that the isotherm obtained from the data fitted the three models used. However, the g parameter from Redlich-Peterson model showed that the isotherm obtained approaches the Freundlich model. This support reached the sorption equilibrium concentration at 3mg PCPg(-1)nylon. To study PCP removal capability by R. oryzae ENHE and to eliminate the error caused by PCP sorbed by the nylon fiber during its quantification, nylon fiber at PCP equilibrium sorption concentration was used to immobilize R. oryzae ENHE. It was found that this fungus grew within nylon fiber cubes in presence or not of PCP, even when PCP caused growth inhibition. Maximum biomass accumulated into nylon cubes without PCP was of 32 mg biomass g(-1)nylon and into nylon cubes at PCP equilibrium concentration was of 18 mg g(-1)nylon. The results showed that R. oryzae ENHE immobilized into nylon fiber removed 88.6% and 92% of PCP in cultures with 12.5 and 25 mg PCPL(-1), as initial concentration, respectively. This is the first work to report that a zygomycete, such as R. oryzae ENHE, immobilized into nylon fiber kept its potential to remove PCP.
    Journal of hazardous materials 06/2011; 190(1-3):707-12. · 4.33 Impact Factor

Publication Stats

533 Citations
203.95 Total Impact Points

Institutions

  • 1997–2014
    • Universidad de Guanajuato
      • Department of Chemistry
      Ciudad Guanajuato, Guanajuato, Mexico
  • 2003–2008
    • University of Cincinnati
      • Department of Chemistry
      Cincinnati, OH, United States
  • 2007
    • University of North Carolina at Chapel Hill
      North Carolina, United States