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ABSTRACT: Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT1-17T and RHLT2-1T) were isolated from Hailuogou glacier in Szechwan province, P. R. China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8 % sequence similarity to each other. The highest level of sequence similarity (97.6 %, 98.4 %, respectively) were found with Nocardioides kribbensis. Strain RHLT1-17T grow at 0-35°C and strain RHLT2-1T grow at 0-25°C. The major cellular fatty acids of strain RHLT1-17T were C17:1ω8c (32.69 %) and iso-C16:0 (21.74 %). The major cellular fatty acids of strain RHLT2-1T were C18:1 ω9c (28.72%), summed feature 3 (17.14 %; comprising C16:1ω7c and/or C16:1ω6c), iso-C16:0 (14.35 %), C16:0 (9.96 %) and iso-C14:0 (8.34 %). Both strains contained LL-2, 6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone. On the basis of a polyphasic approach, two novel species Nocardioides szechwanensis sp. nov. (type strain = RHLT1-17T =CGMCC 1.11147T =NBRC 108562T) and Nocardioides psychrotolerans sp. nov. (type strain = RHLT2-1T =CGMCC 1.11156T =NBRC 108563T) are proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2012; · 2.11 Impact Factor
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ABSTRACT: Strain BZ30(T) was isolated from hydrocarbon-contaminated soil. The Gram-negative, aerobic bacterium was psychrophilic and able to grow at temperatures ranging from 1 to 30 °C. The predominant cellular fatty acids of strain BZ30(T) were summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH) (37.4 %), C(18 : 1)ω7c (19.6 %), C(16 : 0) (8.2 %), C(14 : 0) 2-OH (8.0 %) and C(16 : 0) 2-OH (5.0 %). The predominant ubiquinone was Q-10. Major polar lipids were sphingoglycolipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Spermidine was the major polyamine. The genomic DNA G+C content was 64.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain BZ30(T) belonged to the family Sphingomonadaceae of the α-4 group of the phylum Proteobacteria, and was related to the members of the genus Sphingopyxis, sharing the highest sequence similarities with the type strains of Sphingopyxis chilensis (98.3 %), S. witflariensis (98.2 %), S. taejonensis (97.4 %) and S. ginsengisoli (97.2 %). On the basis of the phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain BZ30(T) represents a novel species of the genus Sphingopyxis, for which the name Sphingopyxis bauzanensis is proposed. The type strain is BZ30(T) (=DSM 22271(T) =CGMCC 1.8959(T) =CIP 110136(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2009; 60(Pt 11):2618-22. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-positive, aerobic bacterium, designated strain BZ41(T), was isolated from hydrocarbon-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ41(T) was related to members of the genus Agromyces and showed highest similarity with the type strain of Agromyces ramosus (96.8 %). The morphological, biochemical and chemotaxonomic characteristics of the new isolate were consistent with the description of the genus Agromyces. The cell-wall peptidoglycan of strain BZ41(T) was of type B2γ and contained the amino acids 2,4-diaminobutyric acid, alanine, glycine and glutamic acid in an approximate molar ratio of 1.8 : 0.7 : 1.1 : 1.0. The predominant cell-wall sugars were galactose, glucose, mannose and rhamnose. Strain BZ41(T) had MK-12 and MK-11 as major menaquinones and contained anteiso-C₁₅:₀ and anteiso-C₁₇:₀ as major fatty acids. The genomic DNA G+C content of strain BZ41(T) was 69.7 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ41(T) is considered to represent a novel species of the genus Agromyces, for which the name Agromyces bauzanensis sp. nov. is proposed. The type strain is BZ41(T) (=DSM 22275(T) =CGMCC 1.8984(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2009; 60(Pt 10):2341-5. · 2.11 Impact Factor
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ABSTRACT: An aerobic, Gram-reaction-positive, non-motile, psychrophilic bacterium, designated strain S6-3(T), was isolated from alpine soil. Cells exhibited a rod-coccus growth cycle and produced a yellow pigment. Growth occurred at 1-25 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S6-3(T) was related to members of the genus Arthrobacter, sharing highest sequence similarities with the type strains of Arthrobacter psychrolactophilus (97.9 %) and Arthrobacter stackebrandtii (97.6 %). Strain S6-3(T) had MK-9(H(2)) as the major menaquinone and anteiso-C(15 : 0) as the major fatty acid. The cell-wall peptidoglycan was of type A3alpha l-Lys-l-Thr-Ala(3). The predominant cell-wall sugars were galactose and rhamnose. The genomic DNA G+C content of strain S6-3(T) was 61.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain S6-3(T) is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter alpinus sp. nov. is proposed. The type strain is S6-3(T) (=DSM 22274(T) =CGMCC 1.8950(T)).
International journal of systematic and evolutionary microbiology 10/2009; 60(Pt 9):2149-53. · 2.27 Impact Factor
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ABSTRACT: A Gram-negative, aerobic bacterium, designated strain BZ26(T), was isolated from hydrocarbon-contaminated soil. The strain was psychrophilic, showing good growth over a temperature range of 1-20 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ26(T) was related to members of the genus Dyadobacter and had highest 16S rRNA gene sequence similarity to Dyadobacter alkalitolerans 12116(T) (98.1 %), Dyadobacter koreensis KCTC 12537(T) (97.5 %) and Dyadobacter ginsengisoli Gsoil 043(T) (97.2 %). Strain BZ26(T) had MK-7 as the major menaquinone and summed feature 3 (C(16 : 1)omega7c/iso-C(15 : 0) 2-OH), C(16 : 1)omega5c and iso-C(15 : 0) as major fatty acids. The genomic DNA G+C content of strain BZ26(T) was 48.9 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ26(T) is considered to represent a novel species of the genus Dyadobacter, for which the name Dyadobacter psychrophilus sp. nov. is proposed. The type strain is BZ26(T) (=DSM 22270(T) =CGMCC 1.8951(T)).
International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 7):1640-3. · 2.27 Impact Factor
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ABSTRACT: Strain BZ92r(T) was isolated from hydrocarbon-contaminated soil. Cells were Gram-negative, aerobic, rod-shaped and cold-adapted (growth at 1-25 degrees C). The major fatty acids were iso-C(15 : 0) (25.6 %), iso-C(17 : 1)omega9c (24.9 %), iso-C(11 : 0) (18.4 %) and iso-C(11 : 0) 3-OH (16.2 %). The predominant ubiquinone was ubiquinone-8. The genomic DNA G+C content was 72.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BZ92r(T) was a member of the genus Luteimonas (94.5-95.2 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain BZ92r(T) was considered to represent a novel species of the genus Luteimonas. The name Luteimonas terricola sp. nov. is proposed, with BZ92r(T) (=DSM 22344(T) =CGMCC 1.8985(T)) as the type strain.
International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 7):1581-4. · 2.27 Impact Factor
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ABSTRACT: An actinomycete, designated MS426T, the culture broth of which showed potent antimicrobial activity, was isolated from a deep-sea sediment sample of the South China Sea. An almost-complete sequence of the 16S rRNA gene of strain MS426T was determined and aligned with those of representatives of the family Micromonosporaceae available in public databases. Phylogenetic trees were inferred by using three algorithms. Strain MS426T formed a branch adjacent to Verrucosispora lutea YIM 013T in a distinct cluster occupied only by strains of the genus Verrucosispora. Strain MS426T was distinguishable from the type strains of the two described Verrucosispora species by using a combination of chemical and morphological markers and by DNA-DNA relatedness. On the basis of these genotypic and phenotypic differences, the novel antimicrobial strain with pharmaceutical potential represents a novel species, for which the name Verrucosispora sediminis sp. nov. is proposed. The type strain is MS426T (=CGMCC 4.3550T =JCM 15670T).
International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 8):1807-12. · 2.27 Impact Factor
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ABSTRACT: A novel cold-living, Gram-stain-negative, yellow-pigmented and obligately aerobic bacterium designated strain 0563(T) was isolated from the China No. 1 glacier. Phylogenetic analysis showed that strain 0563(T) was a member of the genus Flavobacterium, sharing the highest 16S rRNA gene sequence similarities with Flavobacterium limicola JCM 11473(T) (98.5 %) and Flavobacterium psychrolimnae NBRC 102679(T) (98.2 %). The genomic DNA G+C content was 34.8 mol%. Strain 0563(T) displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH, summed feature 3 (comprising C(16 : 1)omega7c and/or C(16 : 1)omega6c), C(15 : 1)omega6c, iso-C(15 : 1) G and iso-C(16 : 0) 3-OH as the major fatty acids. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, the novel species Flavobacterium tiangeerense sp. nov. is proposed; the type strain is 0563(T) (=CGMCC 1.6847(T) =JCM 15087(T)).
International journal of systematic and evolutionary microbiology 08/2009; 59(Pt 11):2773-7. · 2.27 Impact Factor
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ABSTRACT: A novel aerobic, psychrotolerant, yellow-to-orange bacterium (strain 0423(T)) was isolated from the China No. 1 glacier. Strain 0423(T) displayed phenotypic and chemotaxonomic features of the genus Planomicrobium, containing anteiso-C(15 : 0) and iso-C(14 : 0) as the major fatty acids. The temperature range for growth was 4-28 degrees C, with optimum growth at 20-21 degrees C. The genomic DNA G+C content was 49 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0423(T) was related to members of the genus Planomicrobium, sharing the highest sequence similarities with the type strains of Planomicrobium chinense, P. mcmeekinii, P. okeanokoites and P. koreense. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species, Planomicrobium glaciei sp. nov., is proposed. The type strain is 0423(T) (=CGMCC 1.6846(T) =JCM 15088(T)).
International journal of systematic and evolutionary microbiology 07/2009; 59(Pt 6):1387-90. · 2.27 Impact Factor
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ABSTRACT: A Gram-negative-staining bacterium, designated strain 25BT, was isolated from a soil sample from a rice field in Guangxi Province, China, and its taxonomic position was investigated by using a polyphasic approach. Cells were rod-shaped, non-spore-forming, non-motile and strictly aerobic. Strain 25BT grew optimally at 37 degrees C and pH 7.0. The predominant fatty acids of this soil isolate were C18:1omega7c, C19:0 cyclo omega8c and C16:0. Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence showed that strain 25BT formed a monophyletic clade with the type strain of Microvirga subterranea; the two organisms shared 97.2% 16S rRNA gene sequence similarity. However, the two strains shared low DNA-DNA relatedness. Strain 25BT was also readily distinguishable from Microvirga subterranea DSM 14364T by various phenotypic characteristics. The combination of genotypic and phenotypic data suggests that the isolate represents a novel species of the genus Microvirga, for which the name Microvirga guangxiensis sp. nov. is proposed. The type strain is 25BT (=CGMCC 1.7666T=JCM 15710T).
International journal of systematic and evolutionary microbiology 07/2009; 59(Pt 8):1997-2001. · 2.27 Impact Factor
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ABSTRACT: Strain 78-123T was isolated from a sample of a bird's nest situated on the bank of Qiongtailan River in the region of Tuomuer Peak of Tianshan Mountain in the Xin-jiang Uygur Autonomous Region in north-western China. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 78-123T was related to members of the genus Pseudomonas. 16S rRNA gene sequence similarity between strain 78-123T and Pseudomonas mendocina ATCC 25411T, Pseudomonas pseudoalcaligenes JCM 5968T and Pseudomonas alcaliphila AL15-21T was 97.1, 97.4 and 97.5 %, respectively. The major cellular fatty acids were C(16 : 0), C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, C(18 : 1)omega7c and C(12 : 0). The G+C content was 60.4 mol%. On the basis of the phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, the novel species Pseudomonas tuomuerensis sp. nov. is proposed, with the type strain 78-123T (=CGMCC 1.1365T =JCM 14085T).
International journal of systematic and evolutionary microbiology 02/2009; 59(Pt 1):139-43. · 2.27 Impact Factor
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ABSTRACT: A novel psychrophilic, Gram-positive, yellow-pigmented and aerobic bacterium, strain 0543(T), was isolated from the China No. 1 glacier. Strain 0543(T) was able to grow at 4-23 degrees C, with optimum growth at 18-19 degrees C. The major fatty acids were anteiso-C(15 : 0) (58.36 %), iso-C(16 : 0) (21.13 %), iso-C(14 : 0) (10.25 %) and anteiso-C(17 : 0) (7.16 %). The genomic DNA G+C content was 63.5 mol% and the major menaquinone was MK-10. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 0543(T) represented a lineage within the family Microbacteriaceae, with the highest similarity of 97.4 % with Salinibacterium amurskyense KMM 3673(T). On the basis of polyphasic, chemotaxonomic, physiological and biochemical evidence from this study, the novel species Salinibacterium xinjiangense sp. nov. is proposed; the type strain is 0543(T)(=CGMCC 1.5381(T) =JCM 13926(T)).
International journal of systematic and evolutionary microbiology 01/2009; 58(Pt 12):2739-42. · 2.27 Impact Factor
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ABSTRACT: A Gram-positive, psychrophilic, rod-shaped bacterium, designated strain N-05(T), was isolated from soil samples collected off King George Island, west Antarctica (6 degrees 13' 31'' S 5 degrees 57' 08'' W). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N-05(T) was related to members of the genus Sporosarcina and had highest 16S rRNA gene sequence similarity with the type strain of Sporosarcina macmurdoensis (98.0%). The temperature range for growth of strain N-05(T) was 0-23 degrees C, with optimum growth occurring at 17-18 degrees C and approximately pH 6.0-8.0. Strain N-05(T) had MK-7 as the major menaquinone and anteiso-C(15:0) and C(16:1)omega7c alcohol as major fatty acids. The genomic DNA G+C content was 39.2 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain N-05(T) is considered to represent a novel species of the genus Sporosarcina, for which the name Sporosarcina antarctica is proposed. The type strain is N-05(T) (=CGMCC 1.6503(T)=JCM 14646(T)).
International journal of systematic and evolutionary microbiology 10/2008; 58(Pt 9):2114-7. · 2.27 Impact Factor
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ABSTRACT: A novel psychrotolerant, Gram-negative, aerobic bacterium, designated strain 537T, was isolated from sea-ice samples from the Arctic. Strain 537T was able to grow at 4-26 degrees C, with optimum growth occurring at 20-21 degrees C. Strain 537T had Q-8 as the major respiratory quinone and contained iso-C15:0 2-OH and/or C16:1 omega7c (22.95 %), C15:1 (17.64 %) and C17:1 omega8c (13.74 %) as the predominant cellular fatty acids. The genomic DNA G+C content was 38.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 537T formed a coherent cluster within the genus Colwellia. The highest level of 16S rRNA gene sequence similarity (97.5 %) exhibited by strain 537T was obtained with respect to the type strain of Colwellia aestuarii. On the basis of phenotypic, chemotaxonomic and phylogenetic properties and DNA-DNA relatedness data, strain 537T represents a novel species of the genus Colwellia, for which the name Colwellia polaris sp. nov. is proposed. The type strain is 537T (=CGMCC 1.6132T =JCM 13952T).
International journal of systematic and evolutionary microbiology 09/2008; 58(Pt 8):1931-4. · 2.27 Impact Factor
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ABSTRACT: A novel psychrotolerant, Gram-negative, motile bacterium, designated strain 328(T), was isolated from sea-ice samples collected off the Canadian Basin of the Arctic Ocean (7 degrees 23' 14'' N 14 degrees 06' 55'' W). Strain 328(T) was able to grow at 0-37 degrees C, with optimum growth at 25-27 degrees C. It possessed phosphatidylethanolamine and phosphatidylglycerol as major phospholipids and C(10 : 0) 3-OH (31.78 %), C(18 : 1)omega7c (27.50 %) and iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (19.22 %) as predominant cellular fatty acids. The DNA G+C content of strain 328(T) was 45.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 328(T) was a member of the genus Marinomonas (92.7-96.0 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain 328(T) was considered to represent a novel species of the genus Marinomonas. The name Marinomonas arctica sp. nov. is proposed, with strain 328(T) (=CGMCC 1.6498(T)=JCM 14976(T)) as the type strain.
International journal of systematic and evolutionary microbiology 08/2008; 58(Pt 7):1715-8. · 2.27 Impact Factor
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ABSTRACT: Strain 20041(T) was isolated from sea-ice of the Canadian Basin (7 degrees 23' 14'' N 14 degrees 06' 55'' W). Phylogenetic analysis based on 16S rRNA gene homology showed that strain 20041(T) was related to members of the genus Marinobacter and had highest 16S rRNA gene sequence similarity with the type strain of Marinobacter maritimus. Cells were Gram-negative, rod-shaped, psychrophilic and motile. The temperature range for growth was 0-22 degrees C, with optimum growth occurring at 16-18 degrees C and at approximately pH 6.0-9.0. Strain 20041(T) had ubiquinone-9 as the major respiratory quinone and iso-C(15 : 0) 2OH and/or C(16 : 1)omega7c, C(16 : 0), C(18 : 1)omega9c and C(12 : 0) 3OH as major fatty acids. The genomic DNA G+C content was 55.4 mol%. On the basis of the phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain 20041(T) is considered to represent a novel species, for which the name Marinobacter psychrophilus sp. nov. is proposed. The type strain is 20041(T) (=CGMCC 1.6499(T)=JCM 14643(T)).
International journal of systematic and evolutionary microbiology 06/2008; 58(Pt 6):1463-6. · 2.27 Impact Factor
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ABSTRACT: A Gram-negative, psychrophilic, motile, rod-shaped bacterium, designated strain 20188(T), was isolated from marine samples collected from the Arctic (7 degrees 00' 24'' N 16 degrees 59' 37'' W), and was identified taxonomically by means of a polyphasic study. On the basis of 16S rRNA gene sequence similarity, strain 20188(T) was closely related to members of the genera Phaeobacter. 16S rRNA gene sequence similarities between strain 20188(T) and the type strains of Phaeobacter inhibens, Phaeobacter gallaeciensis and Phaeobacter daeponensis were 97.0, 96.8 and 96.2 %, respectively. The temperature range for growth was 0-25 degrees C, with optimum growth occurring at 19-20 degrees C and at approximately pH 6.0-9.0. Strain 20188(T) had ubiquinone-10 as the major respiratory quinone and C(18 : 1)omega7c and 11-methyl C(18 : 1)omega7c as major fatty acids. The genomic DNA G+C content was 59.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain 20188(T) is considered to represent a novel species of the genus Phaeobacter, for which the name Phaeobacter arcticus sp. nov. is proposed. The type strain is 20188(T) (=CGMCC 1.6500(T)=JCM 14644(T)).
International journal of systematic and evolutionary microbiology 06/2008; 58(Pt 6):1384-7. · 2.27 Impact Factor
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ABSTRACT: The taxonomic positions of 'Renobacter vacuolatum' DSM 1277 and 'Ancylobacter polymorphus' DSM 2457 were investigated in this study. 16S rRNA gene sequence analysis indicated that both strains belonged to the genus Ancylobacter. DNA-DNA hybridization showed that they differed from Ancylobacter aquaticus DSM 101(T) and Ancylobacter rudongensis AS 1.1761(T). According to molecular and phenotypic characteristics, strain DSM 1277(T) (=AS 1.2807(T)) is proposed as the type strain of Ancylobacter vacuolatus sp. nov. At the same time, valid publication of the name Ancylobacter polymorphus sp. nov. is proposed, with the type strain DSM 2457(T) (=AS 1.2800(T) = NCIMB 10516(T)).
International journal of systematic and evolutionary microbiology 07/2006; 56(Pt 6):1185-8. · 2.27 Impact Factor
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ABSTRACT: A curved, ring-like bacterium, strain AS 1.1761(T), isolated from the roots of Spartina anglica, was studied by a polyphasic approach. According to phylogenetic analysis, strain AS 1.1761(T) belongs to the genus Ancylobacter, with 99.21 % 16S rDNA sequence similarity to Ancylobacter aquaticus, the only species described so far in this genus. However, strain AS 1.1761(T) had no significant DNA-DNA binding with the type strain of A. aquaticus. In addition, strain AS 1.1761(T) differed from A. aquaticus in many phenotypic features. Based on molecular and phenotypic data, a novel species, Ancylobacter rudongensis sp. nov., is proposed. The type strain is AS 1.1761(T) (=JCM 11671(T)).
International journal of systematic and evolutionary microbiology 04/2004; 54(Pt 2):385-8. · 2.27 Impact Factor
