Shuping Peng

Central South University, Ch’ang-sha-shih, Hunan, China

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Publications (49)104.37 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Our previous study demonstrated that the NGX6b gene acts as a suppressor in the invasion and migration of nasopharyngeal carcinoma (NPC). Recently we identified the novel isoform NGX6a which is longer than NGX6b. In this study, we firstly found that NGX6a was degraded in NPC cells and that this degradation was mediated by ezrin, a linker between membrane proteins and the cytoskeleton. Specific siRNAs against ezrin increase the protein level of NGX6a in these cells. During degradation, NGX6a is not ubiquitinated but is degraded through a proteasome-dependent pathway. The distribution pattern of ezrin was negatively associated with NGX6a in an immunochemistry (IHC) analysis of a nasopharyngeal carcinoma tissue microarray (TMA) and fetus multiple organ tissues and western blot analysis in nasopharyngeal (NP) and NPC cell lines, suggesting that ezrin and NGX6a are associated and are involved in the progression and invasion of NPC. By mapping the interacting binding sites, the seven-trans-membrane domain of NGX6a was found to be the critical region for the degradation of NGX6a, and the amino-terminus of ezrin is required for the induction of NGX6a degradation. The knockdown of ezrin or transfection of the NGX6a mutant CO, which has an EGF-like domain and a trans-membrane 1 domain, resulted in no degradation, significantly reducing the ability of invasion and migration of NPC cells. This study provides a novel molecular mechanism for the low expression of NGX6a in NPC cells and an important molecular event in the process of invasion and metastasis of nasopharyngeal carcinoma cells.
    The Journal of biological chemistry. 11/2014;
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    ABSTRACT: The calcium silicate (CaSiO3) scaffolds added with 0, 10, 20, 30, and 40 wt% nano-zirconia (nano-ZrO2) with controlable porous structure were fabricated via selective laser sintering. The effects of nano-ZrO2 content on the microstructure, crystalline phase, and mechanical and biological properties were investigated. The results showed that the compressive strength and fracture toughness of the scaffolds were enhanced by the addition of nano-ZrO2, and the phase transformation of monoclinic phase (m-ZrO2) into tetragonal phase (t-ZrO2) occurred, which was favorable for the reinforcing ability of ZrO2 due to the stress-induced phase transformation toughening mechanism. However, the excessive amount of nano-ZrO2 would cause undesired agglomeration, poor sinterability, and weak apatite-forming ability. In vitro results showed that there were bone-like apatite layer formation and MG-63 cells attachment on the surfaces of the scaffolds, indicating the scaffolds possessed good biological properties.
    International Journal of Applied Ceramic Technology 10/2014; · 1.15 Impact Factor
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    ABSTRACT: Carcinoma-associated fibroblasts (CAFs) have been demonstrated to play an important role in the occurrence and development of oral squamous cell carcinoma (OSCC). The aim of this study is to investigate the influence of CAFs on OSCC cells and to explore the role of focal adhesion kinase (FAK) in this process. The results showed that oral CAFs expressed a higher level of FAK than normal human gingival fibroblasts (HGFs), and the conditioned medium (CM) of CAFs could induce the invasion and migration of SCC-25, one oral squamous carcinoma cell line. However, knockdown of FAK by small interfering RNA (siRNA) resulted in inhibition of CAF–CM induced cell invasion and migration in SCC-25, probably by reducing the production of monocyte chemoattractant protein-1 (MCP-1/CCL2), one of downstream target chemokines. Therefore, our findings indicated that targeting FAK in CAFs might be a promising strategy for the treatment of OSCC in the future.
    Journal of Biochemical and Molecular Toxicology 09/2014; · 1.60 Impact Factor
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    ABSTRACT: The poor mechanical properties of akermanite (AKM), especially fracture toughness, limits its applications in bone tissue engineering, although it possesses favorable biological performance. In this research, silicon carbide whiskers (SiCw) are added in order to reinforce AKM scaffolds with controllable porous structures fabricated by selective laser sintering (SLS). The mechanical properties, microstructure and toughening mechanisms are analyzed. The results indicate that the compressive strength and fracture toughness increases with increasing SiCw from 5 to 20 wt.%, and then decreases when more SiCw are incorporated. The strengthening and toughening mechanisms are attributed to interface debonding, whisker fracture and whisker pull-out, and the main fracture mode becomes transgranular fracture. Moreover, the phase composition of SiCw remains constant, confirmed by X-ray diffraction (XRD) analysis. The bioactivity and degradation behaviour of the scaffolds were evaluated by soaking them in simulated body fluid (SBF). The results show that the composite scaffolds with 20 wt.% SiCw exhibits good apatite-mineralization ability and a moderate degradation rate in SBF medium. Moreover, scanning electron microscopy (SEM) analysis, MTT assay and alizarin red staining of human bone marrow stromal cells (hBMSCs) seeded scaffolds confirm the stem cell attachment, viability, proliferation and differentiation on the scaffolds. Thus, the overall study proves that SiCw reinforced AKM scaffolds have the potential to be used in bone tissue engineering.
    RSC Advances 08/2014; 4(69). · 3.71 Impact Factor
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    ABSTRACT: Nasopharyngeal carcinoma (NPC) is a major head and neck cancer with high occurrence in Southeast Asia and southern China. To identify novel biomarkers for the early detection of NPC patients, 2D-DIGE combined with MALDI-TOF-MS analysis was performed to identify differentially expressed proteins in the carcinogenesis and progression of NPC using LCM-purified normal nasopharyngeal epithelial tissues and various stages of NPC biopsies. As a result, 26 differentially expressed proteins were identified, of which two proteins with sharp expressional changes in the carcinogenic process, ENO1 and CYPA, were validated by western blot analysis and identified as critical seed proteins in the functional network. Immunohistochemistry assay was further performed to detect the expression of the two proteins with a tissue microarray that included various stages of NPC tissues. The ability of these proteins to detect NPC early was evaluated via a receiver operating characteristic analysis. The results indicated that the combination of the two proteins could perfectly discriminate NNET and AH from stage I of NPC with high sensitivity and specificity, which is more effective than using either of the two proteins individually. In summary, the combination of ENO1 and CYPA can serve as potential molecular markers for the early detection of NPC.
    Journal of proteomics. 07/2014;
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    ABSTRACT: Forsterite-based composite scaffolds with interconnected pore architecture were successfully manufactured via selective laser sintering. The nano-58S bioactive glass (BG) was added to forsterite for purpose of improving the bioactivity of the composite scaffolds. The effect of nano-58S BG contents on the biological behavior and mechanical properties was investigated. The results showed that the composite scaffolds could induce the formation of apatite compared with the pure forsterite scaffolds. Moreover, with increasing nano-58S BG, the scattered spherical apatite particles accumulated continuously and covered the whole surface. At last the apatite layer became sponge-like shape. The presence of apatite was confirmed with scanning electron microscopy equipped with energy dispersive spectroscopy, X-ray diffraction and Fourier transform infrared spectroscopy. In addition, MG-63 cells adhesion was enhanced with increase in the amount of the nano-58S BG. Besides, the maximum compressive strength was 43.9 ± 1.1 MPa when the nano-58S BG was 20.0 wt%. This study indicated that the composite scaffolds have a potential for bone tissue engineering.
    Materials and Manufacturing Processes 07/2014; 29(7). · 1.49 Impact Factor
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    ABSTRACT: Nasopharyngeal carcinoma (NPC) is the most common cancer originating in the nasopharynx, and is extremely common in southern regions of China. Although the standard combination of radiotherapy and chemotherapy has improved the efficiency in patients with NPC, relapse and early metastasis are still the common causes of mortality. Cancer stem-like cells (CSCs) or tumor initial cells are hypothesized to be involved in cancer metastasis and recurrence. Over the past decade, increasing numbers of studies have been carried out to identify CSCs from human NPC cells and tissues. The present paper will summarize the investigations on nasopharyngeal CSCs, including isolation, characteristics, and therapeutic approaches. Although there are still numerous challenges to translate basic research into clinical applications, understanding the molecular details of CSCs is essential for developing effective strategies to prevent the recurrence and metastasis of NPC.
    Stem cell research & therapy. 03/2014; 5(2):44.
  • Pei Feng, Pingpin Wei, Cijun Shuai, Shuping Peng
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    ABSTRACT: A scaffold for bone tissue engineering should have highly interconnected porous structure, appropriate mechanical and biological properties. In this work, we fabricated well-interconnected porous β-tricalcium phosphate (β-TCP) scaffolds via selective laser sintering (SLS). We found that the mechanical and biological properties of the scaffolds were improved by doping of zinc oxide (ZnO). Our data showed that the fracture toughness increased from 1.09 to 1.40 MPam(1/2), and the compressive strength increased from 3.01 to 17.89 MPa when the content of ZnO increased from 0 to 2.5 wt%. It is hypothesized that the increase of ZnO would lead to a reduction in grain size and an increase in density of the strut. However, the fracture toughness and compressive strength decreased with further increasing of ZnO content, which may be due to the sharp increase in grain size. The biocompatibility of the scaffolds was investigated by analyzing the adhesion and the morphology of human osteoblast-like MG-63 cells cultured on the surfaces of the scaffolds. The scaffolds exhibited better and better ability to support cell attachment and proliferation when the content of ZnO increased from 0 to 2.5 wt%. Moreover, a bone like apatite layer formed on the surfaces of the scaffolds after incubation in simulated body fluid (SBF), indicating an ability of osteoinduction and osteoconduction. In summary, interconnected porous β-TCP scaffolds doped with ZnO were successfully fabricated and revealed good mechanical and biological properties, which may be used for bone repair and replacement potentially.
    PLoS ONE 01/2014; 9(1):e87755. · 3.53 Impact Factor
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    ABSTRACT: In this study, nano-hydroxyapatite scaffolds with high mechanical strength and an interconnected porous structure were prepared using NTSS for the first time. The first step was performed using a laser characterized by the rapid heating to skip the surface diffusion and to obtain the driving force for grain boundary diffusion. Additionally, the interconnected porous structure was achieved by SLS. The second step consisted of isothermal heating in a furnace at a lower temperature (T2) than that of the laser beam to further increase the density and to suppress grain growth by exploiting the difference in kinetics between grain-boundary diffusion and grain-boundary migration. The results indicated that the mechanical properties first increased and then decreased as T2 was increased from 1050 to 1250°C. The optimal fracture toughness, compressive strength and stiffness were 1.69 MPam(1/2), 18.68 MPa and 245.79 MPa, respectively. At the optimal point, the T2 was 1100°C, the grain size was 60 nm and the relative density was 97.6%. The decrease in mechanical properties was due to the growth of grains and the decomposition of HAP. The cytocompatibility test results indicated that cells adhered and spread well on the scaffolds. A bone-like apatite layer formed, indicating good bioactivity.
    Scientific reports. 01/2014; 4:5599.
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    ABSTRACT: Calcium silicate possessed excellent biocompatibility, bioactivity and degradability, while the high brittleness limited its application in load-bearing sites. Hydroxyapatite whiskers ranging from 0 to 30 wt.% were incorporated into the calcium silicate matrix to improve the strength and fracture resistance. Porous scaffolds were fabricated by selective laser sintering. The effects of hydroxyapatite whiskers on the mechanical properties and toughening mechanisms were investigated. The results showed that the scaffolds had a uniform and continuous inner network with the pore size ranging between 0.5 mm and 0.8 mm. The mechanical properties were enhanced with increasing hydroxyapatite whiskers, reached a maximum at 20 wt.% (compressive strength: 27.28 MPa, compressive Young's modulus: 156.2 MPa, flexural strength: 15.64 MPa and fracture toughness: 1.43 MPa·m1/2) and then decreased by addition of more hydroxyapatite whiskers. The improvement of mechanical properties was due to whisker pull-out, crack deflection and crack bridging. Moreover, the degradation rate decreased with the increase of hydroxyapatite whisker content. A layer of bone-like apatite was formed on the scaffold surfaces after being soaked in simulated body fluid. Human osteoblast-like MG-63 cells spread well on the scaffolds and proliferated with increasing culture time. These findings suggested that the calcium silicate scaffolds reinforced with hydroxyapatite whiskers showed great potential for bone regeneration and tissue engineering applications.
    Materials Characterization. 01/2014; 97:47–56.
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    ABSTRACT: Carbon nanotubes are ideal candidates for the mechanical reinforcement of ceramic due to their excellent mechanical properties, high aspect ratio and nanometer scale diameter. In this study, the effects of multi-walled carbon nanotubes (MWCNTs) on the mechanical properties of diopside (Di) scaffolds fabricated by selective laser sintering were investigated. Results showed that compressive strength and fracture toughness improved significantly with increasing MWCNTs from 0.5 to 2 wt %, and then declined with increasing MWCNTs to 5 wt %. Compressive strength and fracture toughness were enhanced by 106% and 21%, respectively. The reinforcing mechanisms were identified as crack deflection, MWCNTs crack bridging and pull-out. Further, the scaffolds exhibited good apatite-formation ability and supported adhesion and proliferation of cells in vitro.
    International Journal of Molecular Sciences 01/2014; 15(10):19319-29. · 2.46 Impact Factor
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    ABSTRACT: Fabrication of mechanically competent bioactive scaffolds is a great challenge in bone tissue engineering. In this paper, β-tricalcium phosphate (β-TCP) scaffolds were successfully fabricated by selective laser sintering combined with furnace sintering. Bioglass 45S5 was introduced in the process as liquid phase in order to improve the mechanical and biological properties. The results showed that sintering of β-TCP with the bioglass revealed some features of liquid phase sintering. The optimum amount of 45S5 was 5 wt %. At this point, the scaffolds were densified without defects. The fracture toughness, compressive strength and stiffness were 1.67 MPam1/2, 21.32 MPa and 264.32 MPa, respectively. Bone like apatite layer was formed and the stimulation for apatite formation was increased with increase in 45S5 content after soaking in simulated body fluid, which indicated that 45S5 could improve the bioactivity. Furthermore, MG-63 cells adhered and spread well, and proliferated with increase in the culture time.
    International Journal of Molecular Sciences 01/2014; 15(8):14574-90. · 2.46 Impact Factor
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    ABSTRACT: Akermanite had attracted great attention due to the favourable mechanical properties and excellent biological performance. In this research, the microstructure and mechanical properties of akermanite scaffolds fabricated via laser sintering under different process conditions were studied and characterized. The results showed that the akermanite particles gradually mixed together, grew up and reached complete densification with the scanning speed decreasing from 450 to 150mm/min, while micro defects such as air holes occurred at 50mm/min. simultaneously, the compressive strength of the scaffolds went up and then descended, and the optimum value was 5.92±0.41 MPa. The Vickers hardness and fracture toughness increased consistently and then tended to stabilize. X-ray diffraction (XRD) results indicated no new phase appeared under all process conditions. MG-63 cell culture revealed that cell adhesion and proliferation occurred, indicating excellent cytocompatibility of the scaffolds. Moreover, in vitro bioactivity tests showed that the apatite layer formed on the scaffolds and became dense and thick with the increase of soaking time in simulated body fluid (SBF), and this fact was further confirmed by energy-dispersive spectroscopy (EDS).
    Bio-medical materials and engineering 01/2014; 24(6):2073-80. · 1.09 Impact Factor
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    ABSTRACT: Purpose ‐ The paper aims to fabricate an a-tricalcium phosphate (TCP) scaffold with an interconnected porous structure via selective laser sintering (SLS). To inhibit the phase transformation from ß- to a-TCP in fabrication process of porous scaffolds, a small amount (1 weight per cent) of poly (L-lactic acid) (PLLA) is added into ß-TCP powder to introduce the transient liquid phase. Design/methodology/approach ‐ The paper opted for the transient liquid phase of melting PLLA to decrease the sintering temperature in SLS. Meanwhile, the densification of ß-TCP is enhanced with a combined effect of the capillary force caused by melting PLLA and the surface energy of ß-TCP particles. Moreover, the PLLA will gradually decompose and completely disappear with laser irradiation. Findings ‐ The testing results show the addition of PLLA enables the scaffolds to achieve a higher ß-TCP content of 77 ± 1.49 weight per cent compared with the scaffold sintered from ß-TCP powder (60 ± 1.65 weight per cent), when the laser energy density is 0.4 J/mm2. The paper provides the mechanism of PLLA inhibition on the phase transformation from ß- to a-TCP. And the optimum sintering parameters are obtained based on experimental results, which are used to prepare a TCP scaffold with an interconnected porous structure via SLS. Research limitations/implications ‐ This paper shows that the laser energy density is an important sintering parameter that can provide the means to control the micro-porous structure of the scaffold. If the laser energy density is too low, the densification is not enough. On the other hand, if the laser energy density is too high, the microcracks are observed which are attributed to the volume expansion during the phase transformation from ß- to a-TCP. Therefore, the laser energy density must be optimized. Originality/value ‐ The paper provides a feasible method for fabricating TCP artificial bone scaffold with good biological and mechanical properties.
    Rapid Prototyping Journal 01/2014; 20(5). · 1.00 Impact Factor
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    ABSTRACT: Bioactive ceramics have received great attention in the past decades owing to their success in stimulating cell proliferation, differentiation and bone tissue regeneration. They can react and form chemical bonds with cells and tissues in human body. This paper provides a comprehensive review of the application of bioactive ceramics for bone repair and regeneration. The review systematically summarizes the types and characters of bioactive ceramics, the fabrication methods for nanostructure and hierarchically porous structure, typical toughness methods for ceramic scaffold and corresponding mechanisms such as fiber toughness, whisker toughness and particle toughness. Moreover, greater insights into the mechanisms of interaction between ceramics and cells are provided, as well as the development of ceramic-based composite materials. The development and challenges of bioactive ceramics are also discussed from the perspective of bone repair and regeneration.
    International Journal of Molecular Sciences 01/2014; 15(3):4714-32. · 2.46 Impact Factor
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    ABSTRACT: Graphene is a novel material and currently popular as an enabler for the next-generation nanocomposites. Here, we report the use of graphene to improve the mechanical properties of nano-58S bioactive glass for bone repair and regeneration. And the composite scaffolds were fabricated by a homemade selective laser sintering system. Qualitative and quantitative analysis demonstrated the successful incorporation of graphene into the scaffold without obvious structural damage and weight loss. The optimum compressive strength and fracture toughness reached 48.65 ± 3.19 MPa and 1.94 ± 0.10 MPa·m(1/2) with graphene content of 0.5 wt%, indicating significant improvements by 105% and 38% respectively. The mechanisms of pull-out, crack bridging, crack deflection and crack tip shielding were found to be responsible for the mechanical enhancement. Simulated body fluid and cell culture tests indicated favorable bioactivity and biocompatibility of the composite scaffold. The results suggest a great potential of graphene/nano-58S composite scaffold for bone tissue engineering applications.
    Scientific Reports 01/2014; 4:4712. · 5.08 Impact Factor
  • Acta Biochimica et Biophysica Sinica 12/2013; · 1.81 Impact Factor
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    ABSTRACT: β-Tricalcium phosphates have been widely used as biomaterials for bone substitutes; however, the poor mechanical properties limit the application in bearing loading bones. In this study, nano-hydroxyapatite has been introduced to improve the mechanical properties for porous bioceramic scaffolds. Nanocomposites containing 0–10 wt% needle-like nano-hydroxyapatite were prepared for investigation. It has been found that needle-like nano-hydroxyapatite improves the toughness, hardness, and compressive strength of the porous β-tricalcium phosphates scaffolds, as well as the microstructure properties. The study provides a reference for the development of safe, excellent bone scaffolds for bone tissue engineering.
    International Journal of Applied Ceramic Technology 11/2013; 10(6). · 1.15 Impact Factor
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    ABSTRACT: Biphasic calcium phosphate (BCP), which is composed of hydroxyapatite [HAP, Ca10(PO4)6(OH)2] and β-tricalcium phosphate [β-TCP, β-Ca3(PO4)2], is usually difficult to densify into a solid state with selective laser sintering (SLS) due to the short sintering time. In this study, the sintering ability of BCP ceramics was significantly improved by adding a small amount of polymers, by which a liquid phase was introduced during the sintering process. The effects of the polymer content, laser power and HAP/β-TCP ratios on the microstructure, chemical composition and mechanical properties of the BCP scaffolds were investigated. The results showed that the BCP scaffolds became increasingly more compact with the increase of the poly(l-lactic acid) (PLLA) content (0-1wt.%) and laser power (6-10W). The fracture toughness and micro-hardness of the sintered scaffolds were also improved. Moreover, PLLA could be gradually decomposed in the late sintering stages and eliminated from the final BCP scaffolds if the PLLA content was below a certain value (approximately 1wt.% in this case). The added PLLA could not be completely eliminated when its content was further increased to 1.5wt.% or higher because an unexpected carbon phase was detected in the sintered scaffolds. Furthermore, many pores were observed due to the removal of PLLA. Micro-cracks and micro-pores occurred when the laser power was too high (12W). These defects resulted in a deterioration of the mechanical properties. The hardness and fracture toughness reached maximum values of 490.3±10HV and 1.72±0.10MPam(1/2), respectively, with a PLLA content of approximately 1wt.% and laser power of approximately 10W. Poly(l-lactic-co-glycolic acid) (PLGA) showed similar effects on the sintering process of BCP ceramics. Rectangular, porous BCP scaffolds were fabricated based on the optimum values of the polymer content and laser power. This work may provide an experimental basis for improving the mechanical properties of BCP bone scaffolds fabricated with SLS.
    Materials science & engineering. C, Materials for biological applications. 10/2013; 33(7):3802-10.
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    ABSTRACT: A porous β-tricalcium phosphate (β-TCP) bioceramic scaffold was successfully prepared with our homemade selective laser sintering system. Microstructure observation by a scanning electron microscope showed that the grains grew from 0.21 to 1.32 μm with the decrease of laser scanning speed from 250 to 50 mm min−1. The mechanical properties increased mainly due to the improved apparent density when the laser scanning speed decreased to 150 mm min−1. When the scanning speed was further decreased, the grain size became larger and the mechanical properties severely decreased. The highest Vickers hardness and fracture toughness of the scaffold were 3.59 GPa and 1.16 MPa m1/2, respectively, when laser power was 11 W, spot size was 1 mm in diameter, layer thickness was 0.1–0.2 mm and laser scanning speed was 150 mm min−1. The biocompatibility of these scaffolds was assessed in vitro with MG63 osteoblast-like cells and human bone marrow mesenchymal stem cells. The results showed that all the prepared scaffolds are suitable for cell attachment and differentiation. Moreover, the smaller the grain size, the better the cell biocompatibility. The porous scaffold with a grain size of 0.71 μm was immersed in a simulated body fluid for different days to assess the bioactivity. The surface of the scaffold was covered by a bone-like apatite layer, which indicated that the β-TCP scaffold possesses good bioactivity. These discoveries demonstrated the evolution rule between grain microstructure and the properties that give a useful reference for the fabrication of β-TCP bone scaffolds.
    Science and Technology of Advanced Materials 09/2013; 14(5):055002. · 3.75 Impact Factor

Publication Stats

114 Citations
104.37 Total Impact Points


  • 2005–2014
    • Central South University
      • • Cancer Research Institute
      • • Key Laboratory of Modern Complex Equipment Design and Extreme Manufacturing
      Ch’ang-sha-shih, Hunan, China
  • 2013
    • Medical University of South Carolina
      • Department of Regenerative Medicine and Cell Biology
      Charleston, South Carolina, United States
  • 2010
    • Yale-New Haven Hospital
      New Haven, Connecticut, United States