L Pari

Publications (54)101.96 Total impact

• Article: Protective role of sinapic acid against arsenic: induced toxicity in rats.

  L Pari, A Mohamed Jalaludeen
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  ABSTRACT: Arsenic compounds are classified as toxicants and human carcinogens. Environmental exposure to arsenic imposes a big health issue worldwide. Sinapic acid is a phenylpropanoid compound and is found in various herbal materials and high-bran cereals. It has been reported that sinapic acid has antioxidant efficacy as metal chelators due to the orientation of functional groups. However, it has not yet been examined in experimental animals. In light of this fact, the purpose of this study was to characterize the protective role of sinapic acid against arsenic induced toxicity in rats. Rats were orally treated with arsenic alone (5mg/kg body weight (bw)/day) plus sinapic acid at different doses (10, 20 and 40mg/kg bw/day) for 30days. Hepatotoxicity was measured by the increased activities of serum hepatospecific enzymes namely aspartate transaminase, alanine transaminase, alkaline phosphatase, gamma glutamyl transferase, lactate dehydrogenase and total bilirubin along with increased elevation of lipid peroxidative markers, thiobarbituric acid reactive substances, lipid hydroperoxides, protein carbonyl content and conjugated dienes. The toxic effect of arsenic was also indicated by significantly decreased activities of enzymatic antioxidants like superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase along with non-enzymatic antioxidant like reduced glutathione. Administration of sinapic acid exhibited significant reversal of arsenic induced toxicity in hepatic tissue. The effect at a dose of 40mg/kgbw/day was more pronounced than the other two doses (10 and 20mg/kgbw/day). All these changes were supported by reduction of arsenic concentration and histopathological observations of the liver. These results suggest that sinapic acid has a protective effect over arsenic induced toxicity in rat.
  Chemico-biological interactions 08/2011; 194(1):40-7. · 2.46 Impact Factor
• Article: Beneficial role of naringin, a flavanoid on nickel induced nephrotoxicity in rats.

  K Amudha, L Pari
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  ABSTRACT: This study was conducted to investigate the beneficial role of naringin on nickel induced nephrotoxicity. Nickel (Ni) (20mg/kg body weight (b.w.) was administered intraperitoneally (i.p.) for 20 days. Naringin was administered orally (20, 40 and 80 mg/kg b.w.) with i.p. administration of Ni. Ni administration increased the levels of serum urea, uric acid and creatinine with a significant decrease in creatinine clearance and decreased levels of urea, uric acid and creatinine in urine. The levels of lipid peroxidation markers and nickel concentration in blood and kidney were also increased. While, the activities of enzymic and non-enzymic antioxidants were decreased. Treatment with naringin attenuated the alterations in the renal and urine markers, decreasing lipid peroxidation markers, increasing the antioxidant cascade and decreasing the nickel concentration in blood and kidney. All these changes were supported by histopathological observations. These findings demonstrate that naringin exerts a protective effect against nickel toxicity.
  Chemico-biological interactions 05/2011; 193(1):57-64. · 2.46 Impact Factor
• Article: Antihyperlipidemic effect of chlorogenic acid and tetrahydrocurcumin in rats subjected to diabetogenic agents.

  K Karthikesan, L Pari, V P Menon
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  ABSTRACT: Diabetes mellitus is associated with dyslipidemia, which is a significant risk factor for cardiovascular complications. The present study was carried out to evaluate the effects of tetrahydrocurcumin (THC) and chlorogenic acid (CGA) alone and in combination on alterations in lipids, lipoproteins and enzymes involved in lipid metabolism in streptozotocin (STZ)-nicotinamide (NA)-induced type 2 diabetic rats. A significant (p<0.05) increase in the concentrations of plasma and tissue (liver and kidney) lipids (cholesterol, triglycerides (TGs), free fatty acids (FFAs) and phospholipids (PLs)) and low density and very low-density lipoproteins (LDL and VLDL), and a decrease in the concentration of high-density lipoproteins (HDL) were noticed in STZ administered diabetic rats. In addition, the activity of 3-hydroxy 3-methylglutaryl coenzyme A (HMG-CoA) reductase increased significantly (p<0.05) in the liver and kidney whereas the activities of lipoprotein lipase (LPL) and lecithin cholesterol acyl transferase (LCAT) were decreased significantly (p<0.05) in the plasma of diabetic rats. Combined administration of CGA (5mg/kg b.w.) and THC (80mg/kg b.w.) for 45 days remarkably reduced the STZ-induced changes in lipids, lipoproteins and lipid metabolising enzymes when compared to the effects of CGA or THC alone in diabetic rats. These results indicate that combination of THC and CGA can potentially ameliorate lipid abnormalities in experimental type 2 diabetes.
  Chemico-biological interactions 12/2010; 188(3):643-50. · 2.46 Impact Factor
• Article: Antihyperglycaemic Effect of Scoparia dulcis.: Effect on Key Metabolic Enzymes of Carbohydrate Metabolism in Streptozotocin-Induced Diabetes

  L. Pari, M. Latha
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  ABSTRACT: In experimental diabetes, enzymes of glucose and fatty acid metabolism are markedly altered. We investigated the effect of Scoparia dulcis. L. plant extract on hepatic key metabolic enzymes of carbohydrate metabolism in streptozotocin-induced diabetic rats. Scoparia dulcis. plant extracts (SPEt) (aqueous, ethanol and chloroform) were orally administered at doses of 50, 100 and 200 mg/kg body weight respectively for 3 weeks, after which hexokinase, glucose 6-phosphate dehydrogenase, glucose 6-phosphatase, fructose 1,6-bisphosphatase in liver and glycogen in liver and muscle were assayed. Blood glucose, glucose 6-phosphatase and fructose 1,6-bisphosphatase were significantly increased and plasma insulin, hexokinase, glucose 6-phosphate dehydrogenase and glycogen were significantly decreased in diabetic rats. Diabetic rats treated with SPEt significantly reversed all these changes to near normal. Aqueous extract of Scoparia dulcis. was comparatively better than ethanol and chloroform extracts. These results indicate that the aqueous extract of Scoparia dulcis. showed antihyperglycaemic effect by attenuating the above biochemical alterations in streptozotocin diabetes.
  10/2008; 42(8):570-576.
• Article: Antidiabetic Activity of Cassia auriculata Flowers: Effect on Lipid Peroxidation in Streptozotocin Diabetes Rats

  L. Pari, M. Latha
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  ABSTRACT: Cassia auriculata, commonly known as “Tanner’s cassia,” is widely used in Indian folk medicine for the treatment of diabetes mellitus. Oral administration of 0.45 g/kg body weight of the aqueous extract of the flower for 30 days resulted in a significant reduction in blood glucose and an increase in plasma insulin, but in the case of 0.15 and 0.30 g/kg, was not significant. The aqueous extract also resulted in decreased free radical formation in tissues studied. Thus, this study shows that Cassia auriculata flower extract (CFEt) has hypoglycemic action. The decrease in lipid peroxides and increase in reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S -transferase (GST) clearly show the antioxidant properties of CFEt. The effect of CFEt was most prominently seen in the case of animals given 0.45 g/kg body weight. CFEt was more effective than glibenclamide.
  09/2008; 40(7):512-517.
• Article: Effect of Coccinia indica on Blood Glucose, Insulin and Key Hepatic Enzymes in Experimental Diabetes

  S. Venkateswaran, L. Pari
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  ABSTRACT: Administration of Coccinia indica leaf extract to normal and streptozotocin diabetic animals exhibited significant hypoglycemic and antihyperglycemic effect and reversed biochemical complications. Oral administration of 200mg/ kg of ethanol extract of Coccinia indica leaves (CLEt) to diabetic animals for 45 days resulted in a significant reduction in blood glucose, glycosylated haemoglobin and an increase in total haemoglobin and plasma insulin. Similarly, the administration of CLEt to normal animals resulted in a significant hypoglycemic effect. The activities of hepatic hexokinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase and glucose-6-phosphate dehydrogenase, a lipogenic enzyme, were measured in the liver of normal, diabetic, normal rats separately treated with CLEt and glibenclamide, and diabetic rats treated separately with CLEt and glibenclamide. The activities of the lipogenic enzyme and hexokinase were significantly decreased, whereas the activities of gluconeogenic enzymes were significantly increased in the diabetic liver. Both CLEt and glibenclamide were able to restore the altered enzyme activities to almost control levels. CLEt was more effective than glibenclamide. The results indicate that the administration of CLEt to diabetic animals normalizes blood glucose and causes marked improvement of altered carbohydrate metabolic enzymes during diabetes.
  09/2008; 40(3):165-170.
• Article: Efficacy of caffeic acid in preventing nickel induced oxidative damage in liver of rats.

  L Pari, A Prasath
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  ABSTRACT: Nickel (Ni), a major environmental pollutant, is known for its wide toxic manifestations. In the present study caffeic acid (CA), one of the most commonly occurring phenolic acids in fruits, grains and dietary supplements, was evaluated for its protective effect against the Ni induced oxidative damage in liver. In this investigation, Ni (20 mg/kg body weight) was administered intraperitoneally for 20 days to induce toxicity. CA was administered orally (15, 30 and 60 mg/kg body weight) for 20 days with intraperitoneal administration of Ni. Ni induced liver damage was clearly shown by the increased activities of serum hepatic enzymes namely aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT) and lactate dehydrogenase (LDH) along with increased elevation of lipid peroxidation indices (thiobarbituric reactive acid substances (TBARS) and lipid hydroperoxides). The toxic effect of Ni was also indicated by significantly decreased levels of enzymatic (superoxide dismutase (SOD), catalase (CAT) glutathione peroxidase (GPx) and glutathione S-transferase (GST)) and non-enzymatic antioxidants (glutathione (GSH), vitamin C and vitamin E). CA administered at a dose of 60 mg/kg body weight significantly reversed the activities of hepatic marker enzymes to their near normal levels when compared with other two doses. In addition, CA significantly reduced lipid peroxidation and restored the levels of antioxidant defense in the liver. All these changes were supported by histological observations. The results indicate that CA may be beneficial in ameliorating the Ni induced oxidative damage in the liver of rats.
  Chemico-Biological Interactions 06/2008; 173(2):77-83. · 2.46 Impact Factor
• Article: Protective role of caffeic acid against alcohol-induced biochemical changes in rats.

  L Pari, K Karthikesan
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  ABSTRACT: Caffeic acid is a well-known phenolic compound mainly present in plants. In this study, caffeic acid was evaluated for its protective effect against chronic ethanol-induced biochemical changes in male Wistar rats. Administration of ethanol (7.9 g/kg/day) for 45 days induced liver and kidney damage as manifested by a significant increase in the levels of serum hepatic and renal markers namely aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), bilirubin, urea, creatinine, and a significant decrease in creatinine clearance and levels of hemoglobin. Plasma thiobarbituric acid-reactive substances and hydroperoxide were significantly elevated where as the levels of nonenzymic antioxidants [reduced glutathione, vitamin E and vitamin C] were significantly decreased in alcohol-intoxicated rats. Administration of caffeic acid along with alcohol significantly decreased the serum levels of liver and kidney markers to near-normal levels. In addition, administration of caffeic acid significantly decreased the levels of lipid peroxidation markers while the levels of antioxidants were significantly increased in circulation of alcohol-fed rats. All these results were accompanied by histological observations in liver. The results demonstrate that caffeic acid has a beneficial effect in reducing the adverse effect of alcohol.
  Fundamental and Clinical Pharmacology 09/2007; 21(4):355-61. · 1.80 Impact Factor
• Article: Beneficial effect of succinic acid monoethyl ester on erythrocyte membrane bound enzymes and antioxidant status in streptozotocin-nicotinamide induced type 2 diabetes.

  L Pari, R Saravanan
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  ABSTRACT: Succinic acid monoethyl ester (EMS) was recently proposed as an insulinotropic agent for the treatment of non-insulin dependent diabetes mellitus. In the present study the effect of EMS and metformin on erythrocyte membrane bound enzymes and antioxidants activity in plasma and erythrocytes of streptozotocin-nicotinamide induced type 2 diabeteic model was investigated. Succinic acid monoethyl ester was administered intraperitonially for 30 days to control and diabetic rats. The effect of EMS on glucose, insulin, hemoglobin, glycosylated hemoglobin, TBARS, hydroperoxide, superoxide dismutase (SOD), catalase (CAT), glutathione peroxide (Gpx), glutathione-S-transferase (GST), vitamins C and E, reduced glutathione (GSH) and membrane bound enzymes were studied. The effect of EMS was compared with metformin, a reference drug. The levels of glucose, glycosylated hemoglobin, TBARS, hyderoperoxide, and vitamin E were increased significantly whereas the level of insulin and hemoglobin, as well as antioxidants (SOD, CAT, Gpx, GST, vitamin C and GSH) membrane bound total ATPase, Na(+)/K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase were decreased significantly in streptozotocin-nicotinamide diabetic rats. Administration of EMS to diabetic rats showed a decrease in the levels of glucose, glycosylated hemoglobin, lipid peroxidation markers and vitamin E. In addition the levels of insulin, hemoglobin, enzymic antioxidants, vitamin C, and GSH and the activities of membrane bound enzymes also were increased in EMS and metformin treated diabetic rats. The present study indicates that the EMS possesses a significant beneficial effect on erythrocyte membrane bound enzymes and antioxidants defense system in addition to its antidiabetic effect.
  Chemico-Biological Interactions 09/2007; 169(1):15-24. · 2.46 Impact Factor
• Article: Effects of diallyl tetrasulfide on cadmium-induced oxidative damage in the liver of rats.

  P Murugavel, L Pari
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  ABSTRACT: The protective efficacy of diallyl tetrasulfide (DTS) from garlic on liver injury induced by cadmium (Cd) was investigated. In this study, Cd (3 mg/kg body weight) was administered subcutaneously for 3 weeks to induce toxicity. DTS was administered orally (10, 20 and 40 mg/kg body weight) for 3 weeks with subcutaneous (sc) injection of Cd. Cd-induced liver damage was evidenced from increased activities of serum hepatic enzymes, namely aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate dehydrogenase, with significant elevation of lipid peroxidation indices (thiobarbituric acid reactive substances and hydroperoxides) and protein carbonyl groups in the liver. Rats subjected to Cd toxicity also showed a decline in the levels of total thiols, reduced glutathione (GSH), vitamin C and vitamin E, accompanied by an increased accumulation of Cd, and significantly decreased activities of superoxide dismutase, catalase (CAT), glutathione peroxidase, glutathione-S-transferase (GST), glutathione reductase, and glucose-6-phosphate dehydrogenase in the liver. Administration of DTS at 40 mg/kg body weight significantly normalised the activities of hepatic marker enzymes, compared to other doses of DTS (10 and 20 mg/kg body weight). In addition, DTS (40 mg/kg body weight) significantly reduced the accumulation of Cd and the level of lipid peroxidation, and restored the level of antioxidant defense in the liver. Histological studies also showed that administration of DTS to Cd-treated rats resulted in a marked improvement of hepatocytes morphology with mild portal inflammation. Our results suggest that DTS might play a vital role in protecting Cd-induced oxidative damage in the liver.
  Human &amp Experimental Toxicology 07/2007; 26(6):527-34. · 1.77 Impact Factor
• Article: Diallyl tetrasulfide modulates the cadmium-induced impairment of membrane bound enzymes in rats.

  P Murugavel, L Pari
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  ABSTRACT: Cadmium (Cd) induces extensive membrane damage that contributes to the cytotoxic effect of Cd. We studied the effect of diallyl tetrasulfide (DTS) from garlic on Cd-induced changes in lipid peroxidation and membrane-bound enzymes in liver, kidney, and testis of rats. Cadmium exposure (3 mg/kg body weight, s.c) for 3 weeks induced a significant elevation in the levels of lipid peroxidation markers (thiobarbituric acid substances and lipid hydroperoxides) with a significant decrease in the activities of membrane bound ATPases (Na+/K+ ATPase, Ca2+ ATPase, Mg2+ ATPase), the indicators of membrane function in liver, kidney and testis. The oral administration of DTS (40 mg/kg body weight) along with Cd significantly decreased the level of lipid peroxidation and significantly restored the activities of membrane bound ATPases. The results of our study suggest that DTS attenuates lipid peroxidation in tissues and promotes the stability of the membrane by protecting it from Cd-induced alterations.
  Journal of basic and clinical physiology and pharmacology 02/2007; 18(1):37-48.
• Article: Cytoprotective and antioxidant role of diallyl tetrasulfide on cadmium induced renal injury: an in vivo and in vitro study.

  L Pari, P Murugavel, S L Sitasawad, K Sandeep Kumar
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  ABSTRACT: Cadmium (Cd) is an environmental and industrial pollutant that affects various organs in humans and animals. A body of evidence has accumulated implicating the free radical generation with subsequent oxidative stress in the biochemical and molecular mechanisms of Cd toxicity. Since kidney is the critical target of Cd toxicity, we carried out this study to investigate the effects of diallyl tetrasulfide (DTS), an organosulfur compound derived from garlic on Cd induced toxicity in the kidney of rats and also in the kidney cell line (vero cells). In experimental rats, subcutaneous administration of Cd (3 mg/kg bw/day) for 3 weeks induced renal damage, which was evident from significantly increased levels of serum urea and creatinine with significant decrease in creatinine clearance. A markedly increased levels of lipid peroxidation markers (thiobarbituric acid reactive substances and lipid hydroperoxides) and protein carbonyl contents with significant decrease in nonenzymic antioxidants (total sulphydryl groups, reduced glutathione, vitamin C and vitamin E) and enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase) as well as glutathione metabolizing enzymes (glutathione reductase, and glucose-6-phosphate dehydrogenase) were also observed in Cd intoxicated rats. Coadministration of DTS (40 mg/kg bw/day) and Cd resulted in the reversal of the kidney function accompanied by a significant decrease in lipid peroxidation and increase in the antioxidant defense system. In vitro studies with vero cells showed that incubation of DTS (5-50 microg/ml) with Cd (10 microM) significantly reduced the cell death induced by Cd. DTS at 40 microg/ml effectively blocked the cell death and lipid peroxidation induced by Cd (10 microM) indicating its cytoprotective property. Further, the flow cytometric assessment on the level of intracellular reactive oxygen species using a fluorescent probe 2', 7'-dichlorofluorescein diacetate (DCF-DA) confirmed the Cd induced intracellular oxidative stress in vero cells, which was significantly suppressed by DTS (40 microg/ml). The histopathological studies in the kidney of rats also showed that DTS (40 mg/kg bw/day) markedly reduced the toxicity of Cd and preserved the architecture of renal tissue. The present study suggests that the cytoprotective potential of DTS in Cd toxicity might be due to its antioxidant and metal chelating properties, which could be useful for achieving optimum effects in Cd induced renal damage.
  Life Sciences 02/2007; 80(7):650-8. · 2.53 Impact Factor
• Article: The antioxidant role of pterostilbene in streptozotocin-nicotinamide-induced type 2 diabetes mellitus in Wistar rats.

  M Amarnath Satheesh, L Pari
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  ABSTRACT: The antioxidant effect of pterostilbene on streptozotocin-nicotinamide-induced diabetic rats has been assessed. The activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and reduced glutathione was significantly decreased in liver and kidney of diabetic animals when compared with normal control. There were significant improvements in these activities after treatment with pterostilbene at a dose of 40 mg kg(-1) for six weeks. The increased levels of lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) in liver and kidney of diabetic rats were also normalized by treatment with pterostilbene. Chronic treatment of pterostilbene remarkably reduced the pathological changes observed in liver and kidney of diabetic rats. These results indicated the antioxidant property of pterostilbene.
  Journal of Pharmacy and Pharmacology 12/2006; 58(11):1483-90. · 2.17 Impact Factor
• Article: Effect of N-benzoyl-D-phenylalanine and metformin on insulin receptors in neonatal streptozotocin-induced diabetic rats: studies on insulin binding to erythrocytes.

  N Ashokkumar, L Pari, Ch Appa Rao
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  ABSTRACT: In the present study, we focused on the insulin-receptor binding in circulating erythrocytes of N-benzoyl-D-phenylalanine (NBDP) and metformin in neonatal streptozotocin (nSTZ)-induced male Wistar rats. We measured blood levels of glucose and plasma insulin and the binding of insulin to cell-membrane ER receptors in NBDP and metformin-treated diabetic rats. The mean specific binding of insulin to ER was significantly lower in diabetic control rats (DC) (53.0 +/- 3.1%) than in NBDP (62.0 +/- 3.1%), metformin (66.0 +/- 3.3%) and NBDP and metformin combination-treated (72.0 +/- 4.2%) diabetic rats, resulting in a significant decrease in plasma insulin. Scatchard plot analysis demonstrated that the decrease in insulin binding was accounted for by a lower number of insulin receptor sites per cell in DC rats when compared with NBDP and metformin-treated rats. High-affinity (Kd1), low-affinity (Kd2), and kinetic analysis revealed an increase in the average receptor affinity in ER from NBDP and metformin-treated diabetic rats having NBDP 2.0 +/- 0.10 x 10(-10) M(-1) (Kd1); 12.0 +/- 0.85 x 10(-8) M(-1) (Kd2), Metformin 2.1 +/- 0.15 x 10(-10) M(-1) (Kd1); 15.0 +/- 0.80 x 10(-8) M(-1) (Kd2), NBDP and metformin 2.7 +/- 0.10 x 10(-10) M(-1) (Kd1); 20.0 +/- 1.2 x 10(-8) M(-1) (Kd2) compared with 0.9 +/- 0.06 x 10(-10) M(-1) (Kd1); 6.0 +/- 0.30 x 10(-8) M(-1) (Kd2) in DC rats. The results suggest an acute alteration in the number of insulin receptors on ER membranes in nSTZ induced diabetic control rats. Treatment with NBDP along with metformin significantly improved specific insulin binding, with receptor number and affinity binding reaching almost normal non-diabetic levels. The data presented here show that NBDP along with metformin increase total ER membrane insulin binding sites with a concomitant significant increase in plasma insulin.
  Archives of Physiology and Biochemistry 08/2006; 112(3):174-81.
• Article: Effect of pterostilbene on hepatic key enzymes of glucose metabolism in streptozotocin- and nicotinamide-induced diabetic rats.

  L Pari, M Amarnath Satheesh
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  ABSTRACT: The purpose of this study was to investigate the effect of pterostilbene and its effect on key enzymes of glucose metabolism. Diabetic rats were orally administered with pterostilbene (10, 20, 40 mg/kg) for 2, 4 and 6 weeks on glucose was determined. Administration of pterostilbene at 40 mg/kg significantly decreases plasma glucose. Based on these data, the higher dose, 40 mg/kg pterostilbene, was selected for further evaluation. Oral administration of pterostilbene for 6 weeks on glucose, insulin levels and hepatic enzymes in normal and streptozotocin (STZ)-nicotinamide-induced diabetic rats. A significant decrease in glucose and significant increase in plasma insulin levels were observed in normal and diabetic rats treated with pterostilbene. Treatment with pterostilbene resulted in a significant reduction of glycosylated hemoglobin and an increase in total hemoglobin level. The activities of the hepatic enzymes such as hexokinase was significantly increased whereas glucose-6-phosphatase, fructose-1,6-bisphosphatase were significantly decreased by the administration of pterostilbene in diabetic rats. A comparison was made between the action of pterostilbene and the antidiabetic drug--metformin.
  Life Sciences 08/2006; 79(7):641-5. · 2.53 Impact Factor
• Article: Phytochemical and antimicrobial study of an antidiabetic plant: Scoparia dulcis L.

  M Latha, K M Ramkumar, L Pari, P N Damodaran, V Rajeshkannan, T Suresh
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  ABSTRACT: The antimicrobial and antifungal effects of different concentrations of chloroform/methanol fractions of Scoparia dulcis were investigated. The isolated fractions were tested against different bacteria like Salmonella typhii, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Proteus vulgaris and fungal strains such as Alternaria macrospora, Candida albicans, Aspergillus niger, and Fusarium oxysporum. The isolated fractions exhibited significant antimicrobial and antifungal activity against all the tested organisms compared with respective reference drugs. The isolated fractions of S. dulcis showed properties like antimicrobial and antifungal activities that will enable researchers in turn to look for application-oriented principles.
  Journal of Medicinal Food 02/2006; 9(3):391-4. · 1.41 Impact Factor
• Article: Effect of N-benzoyl-D-phenylalanine on streptozotocin-induced changes in the lipid and lipoprotein profile in rats.

  N Ashokkumar, L Pari, A Manimekalai, K Selvaraju
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  ABSTRACT: The effect of N-benzoyl-D-phenylalanine (NBDP) and metformin combination treatment on circulatory lipids, lipoproteins and lipid peroxidation markers were studied in neonatal streptozotocin (nSTZ) non-insulin dependent diabetic rats. Non-insulin dependent diabetes mellitus (NIDDM) was induced by a single dose injection of streptozotocin (100 mg kg(-1), i. p.) to two-day-old rats. After 10-12 weeks, rats weighing above 150 g were selected for screening for the NIDDM model. The rats were checked for fasting blood glucose levels to confirm the status of NIDDM. NBDP (50,100 or 200 mg kg(-1) ) was administered orally for six weeks to the confirmed diabetic rats (to evaluate the effective dose). The levels of serum lipids and lipid peroxidation markers were significantly increased, whilst the activity of glucose-6-phosphate dehydrogenase was significantly decreased in nSTZ diabetic rats. NBDP and metformin were able to restore the altered serum lipids, lipoproteins, lipid peroxidation marker levels and glucose-6-phosphate dehydrogenase activity to almost control levels. The results showed the antihyperlipidaemic properties of NBDP and metformin in addition to its antidiabetic action. Combination treatment was more effective then either drug alone. The results indicated that the coadministration of NBDP with metformin to nSTZ diabetic rats normalized blood glucose and caused marked improvement in altered serum lipids, lipoproteins and lipid peroxidation markers during diabetes. The data indicated that NBDP represented an effective antihyperglycaemic and antihyperlipidaemic adjunct for the treatment of diabetes, and may be a potential source of new orally active agents for future therapy.
  Journal of Pharmacy and Pharmacology 04/2005; 57(3):359-66. · 2.17 Impact Factor
• Article: Antidiabetic effect of Scoparia dulcis: effect on lipid peroxidation in streptozotocin diabetes.

  L Pari, M Latha
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  ABSTRACT: Oxidative damage has been suggested to be a contributory factor in the development and complications of diabetes. The antioxidant effect of an aqueous extract of Scoparia dulcis, an indigenous plant used in Ayurvedic medicine in India was studied in rats with streptozotocin-induced diabetes. Oral administration of Scoparia dulcis plant extract (SPEt) (200 mg/kg body weight) for 3 weeks resulted in a significant reduction in blood glucose and an increase in plasma insulin. The aqueous extract also resulted in decreased free radical formation in tissues (liver and kidney) studied. The decrease in thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HPX) and increase in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and glutathione-S-transferase (GST) clearly show the antioxidant properties of SPEt in addition to its antidiabetic effect. The effect of SPEt at 200 mg/kg body weight was better than glibenclamide, a reference drug.
  General Physiology and Biophysics 04/2005; 24(1):13-26. · 1.19 Impact Factor
• Article: Effect of an aqueous extract of Scoparia dulcis on plasma and tissue glycoproteins in streptozotocin induced diabetic rats.

  M Latha, L Pari
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  ABSTRACT: The influence of Scoparia dulcis, a traditionally used plant for the treatment of diabetes mellitus, was examined in streptozotocin diabetic rats on dearrangement in glycoprotein levels. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of streptozotocin. An aqueous extract of Scoparia dulcis plant was administered orally for 6 weeks. The effect of the Scoparia dulcis extract on blood glucose, plasma insulin, plasma and tissue glycoproteins studied was in comparison to glibenclamide. The levels of blood glucose and plasma glycoproteins were increased significantly whereas the level of plasma insulin was significantly decreased in diabetic rats. There was a significant decrease in the level of sialic acid and elevated levels of hexose, hexosamine and fucose in the liver and kidney of streptozotocin diabetic rats. Oral administration of Scoparia dulcis plant extract (SPEt) to diabetic rats led to decreased levels of blood glucose and plasma glycoproteins. The levels of plasma insulin and tissue sialic acid were increased whereas the levels of tissue hexose, hexosamine and fucose were near normal. The present study indicates that Scoparia dulcis possesses a significant beneficial effect on glycoproteins in addition to its antidiabetic effect.
  Pharmazie 03/2005; 60(2):151-4. · 1.01 Impact Factor
• Article: Antioxidant effect of Boerhavia diffusa L. in tissues of alloxan induced diabetic rats.

  M Amarnath Satheesh, L Pari
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  ABSTRACT: Administration of B. diffusa leaf extract (BLEt; 200 mg/kg) for 4 weeks resulted in a significant reduction in thiobarbutric acid reactive substances and hydroperoxides, with a significant increase in reduced glutathione, superoxide dismutase, catalase, glutathione peroxidase and glutathione--S-transferase in liver and kidney of alloxan induced diabetic rats. The results suggest that BLEt has remarkable antidiabetic activity and can improve antioxidant status in alloxan induced diabetic rats.
  Indian journal of experimental biology 11/2004; 42(10):989-92. · 1.29 Impact Factor