J F García Marín

Universidad de León, León, Castille and León, Spain

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Publications (62)101.33 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In 2011, neurological disease was reported in a herd of goats (Capra hircus) in Asturias, Spain. Initial sequencing identified the causative agent as louping ill virus (LIV). Subsequently, with the application of whole genome sequencing and phylogenic analysis, empirical data demonstrates that the LIV-like virus detected is significantly divergent from LIV and Spanish sheep encephalitis virus (SSEV). This virus encoded an amino acid sequence motif at the site of a previously identified marker for differentiating tick-borne flaviviruses, that was shared with a virus previously isolated in Ireland in 1968. The significance of these observations reflects the diversity of tick-borne flaviviruses in Europe. These data also contribute to our knowledge of the evolution of tick-borne flaviviruses and could reflect the movement of viruses throughout Europe. Based on these observations, the proposed name for this virus is Spanish goat encephalitis virus (SGEV), to distinguish it from SSEV.
    Journal of General Virology 02/2015; 96(7). DOI:10.1099/vir.0.000096 · 3.18 Impact Factor
  • E. Gayo · A. Balseiro · J. González · L.J. Royo · J.F. García Marín
    Journal of Comparative Pathology 01/2015; 152(1):86. DOI:10.1016/j.jcpa.2014.10.180 · 1.14 Impact Factor
  • Journal of Comparative Pathology 01/2015; 152(1). DOI:10.1016/j.jcpa.2014.10.013 · 1.14 Impact Factor
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    ABSTRACT: Bovine tuberculosis (TB) infection is infrequently diagnosed in sheep. Most reports are from single individual cases or flock outbreaks. However, in Spain several outbreaks have been reported recently, all of which had epidemiological links with TB-infected cattle herds. A total of 897 sheep suspected of being infected with TB and belonging to 23 flocks cohabiting with TB-infected cattle herds and/or goats were tested between 2009 and 2013 in Galicia (north-western Spain), using pathological, immunological and molecular techniques. Of these, 50.44% were positive by culture, 83.23% by histopathology and 24.92%, 4.86% and 59.42% by single intradermal tuberculin test (SITT), interferon-γ and ELISA, respectively. Results suggest that in circumstances akin to those in our study, sheep may be considered as a potential source of TB. We conclude that under similar conditions, serious consideration should be given to TB testing sheep, as they may represent a potential risk to other susceptible co-habiting species. The SITT and ELISA are recommended as the simplest and most cost-effective initial approaches for the diagnosis of TB in sheep under field conditions. However, when possible, interferon-γ should be applied to increase sensitivity.
    Transboundary and Emerging Diseases 01/2015; DOI:10.1111/tbed.12325 · 2.94 Impact Factor
  • J.F. García Marín · L. Polledo · C. Zapico · A. Balseiro
    Journal of Comparative Pathology 01/2014; 150(1):124. DOI:10.1016/j.jcpa.2013.11.200 · 1.14 Impact Factor
  • A. Balseiro · A. Oleaga · L. Polledo · G. Aduriz · R. Atxaerandio · N. Cortabarria · J.F. García Marín
    Journal of Comparative Pathology 01/2014; 150(1):125. DOI:10.1016/j.jcpa.2013.11.201 · 1.14 Impact Factor
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    ABSTRACT: The two main genotypes of recognized isolates of Mycobacterium avium subsp. paratuberculosis (Map) are cattle (C) and sheep (S) strains. An experimental infection was conducted to establish the effect of Map strain on the pathogenesis of ovine paratuberculosis. Twenty-four out of thirty 1.5-month-old Assaf lambs were divided into 4 groups of 6 and infected orally with three low passage field isolates, two of S- (22G and the pigmented Ovicap49) and one of C- (764) type, and the reference K-10 strain (C type). The remaining six animals were unchallenged controls. Animals were euthanized at 150 and 390 days post-infection (dpi). Throughout the experiment, the peripheral immune response was assessed and histological and molecular (PCR) studies were conducted on samples of intestine and related lymphoid tissue. Specific antibody and IFN-gamma production was significantly higher in animals infected with the C strains, while no consistent IFN- gamma responses were observed in the S-type strain infected groups. A positive intradermal skin test response was detected in all infected groups. Lambs infected with S-type strains had granulomatous lesions restricted to the lymphoid tissue with no differences in the lesion intensity over time. In both C-type strain groups, lesions were more severe at 150 dpi while at 390 dpi lesions, characterized by well-demarcated granulomas with fibrosis, decreased in severity. Only infected lambs were positive to PCR. These results suggest that the strain of Map has a strong influence over the immune and pathological responses developed by the host. Lesions induced by C-type strains in lambs show a regressive character and tend to decrease as the infection progresses.
    Veterinary Research 01/2014; 45(1):5. DOI:10.1186/1297-9716-45-5 · 2.82 Impact Factor
  • A Balseiro · L J Royo · A Gómez Antona · J F García Marín
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    ABSTRACT: Between January and June 2013, nine stillborn bovine foetuses with congenital malformations from nine cattle herds located in Salamanca (central Spain) were detected. Necropsy was performed on two calves. Pathological lesions together with molecular genetics and serological results allowed a definitive diagnosis: first confirmation of Schmallenberg virus (SBV) infection in cattle in Spain. SBV was detected in different tissues and organic fluids in both animals including blood, suggesting a possible viraemia. The umbilical cord was also positive for the presence of SBV in both animals. The former tissue provides an easy to obtain sample and might be a sample of choice when necropsy is carried out in the field.
    Transboundary and Emerging Diseases 11/2013; 62(5). DOI:10.1111/tbed.12185 · 2.94 Impact Factor
  • Ana Balseiro · Luis J. Royo · Antonio Gómez Antona · Juan F. García Marín
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    ABSTRACT: Abstract Clostridium sordellii is found in the environment and occasionally in animal (including human) intestines and may cause myonecrosis and large outbreaks of enterotoxemia. A few cases of fatal clostridial infection in bears (Ursus spp.) have been described worldwide but none attributed to C. sordellii. We describe a fatal case of septicemia caused by C. sordellii in an illegally trapped brown bear (Ursus arctos). At necropsy, acute gangrenous myositis was the primary lesion. Serohemorrhagic edema was observed in the abdominal cavity, thorax, pericardium, and skeletal muscle, mostly affecting femoral, humeral, and scapular muscles. Hemorrhage was observed in the heart, skeletal muscles, stomach, and intestine. Liver, spleen, and kidney appeared with loss of consistency, hemorrhages, and edema. Microscopically, primary lesions were in skeletal muscle, stomach, and small intestine, with gram-positive, clostridial-like bacilli. Biochemical and molecular tests identified C. sordellii in cultures from liver, muscle, and intestine. Sequences showed a homology of >99% with the 16S rRNA gene sequence of C. sordellii. The severity of effects of the C. sordellii infection reveal the importance of this pathogen as a wildlife health risk with conservation concerns, as well as the need to consider possible infection with this pathogen in management actions involving immobilization, stress, or severe muscular activity of wild brown bears.
    Journal of wildlife diseases 10/2013; 49(4):1047-51. DOI:10.7589/2013-03-065 · 1.36 Impact Factor
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    ABSTRACT: The aim of this work was to study a retrospective case series of acute degenerative myopathy, which caused high mortality in adult horses grazing in a specific region of Spain, in relation to a possible etiopathogenesis. Outbreaks of myopathy occurred in December of 1999, 2003, 2009, and 2011 after an abrupt fall in temperatures and the first snowfalls. Ten horses were necropsied, and an exhaustive gross and histopathological examination was performed using specific histochemical stainings to evaluate muscle and cardiac damage. Intense myodegeneration, affecting the postural and respiratory muscles and the muscles involved in swallowing, was the main finding. A further consistent finding was necrosis of Purkinje fibers in the myocardium. Serum concentration values of creatine kinase, selenium, and vitamin E, as well as blood concentration values of glutathione peroxidase activity, were determined in 10 horses clinically affected and in another 12 horses with no clinical signs. In the affected horses, creatine kinase was high and reached values over 10,000 U/L. Selenium values were deficient in the horses affected, ranging from 4.2 to 10.5 μg/g (reference range: >50 μg/L) in selenium-untreated horses, and also, selenium values in 11 horses with no clinical signs were below the reference range. Glutathione peroxidase activity was below the reference range in all the analyzed sera, and vitamin E values were also below the reference range in four horses. The severe selenium deficiency together with a sudden arrival of unfavorable weather conditions seemed to provoke the arising of this disease. The presence of Eupatorium cannabinum in this valley is discussed as a possible source of the phytotoxin associated with rhabdomyolysis.
    Journal of Equine Veterinary Science 06/2013; 33(6):475–482. DOI:10.1016/j.jevs.2012.07.017 · 0.87 Impact Factor
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    ABSTRACT: Maedi-visna (MV) is a slow lentiviral disease of sheep that has a significant economic impact in many sheep-producing regions although there remains a paucity of data relating to actual production losses resulting from this disease. The objective of this study was to evaluate direct losses, through death or culling, from two dairy sheep flocks with high seroprevalences of infection over a 2year period. Maedi-visna was found, either alone or in combination with other diseases, to be the most common disease diagnosed in these sheep, and the major cause of direct animal losses in the two flocks. Moderate to severe lesions associated with MV were found in 52% and 80% of the sheep, respectively, affecting the lungs, brain and/or mammary glands. Despite the similarity of the two flocks under study in terms of breed, number of animals, geographical proximity, and inter-change of rams, a striking difference was observed regarding the clinical presentation of the disease: in one flock the respiratory form was dominant while in the other 70% of animals died or were culled because of neurological signs.
    The Veterinary Journal 05/2013; 197(3). DOI:10.1016/j.tvjl.2013.03.031 · 1.76 Impact Factor
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    ABSTRACT: The presence of Maedi-Visna disease in sheep flocks have a significant economic impact, thus the aim of this study was to carry out a serological evaluation of a simple epidemiological control method for ovine Maedi-Visna infection in an intensive dairy flock with high initial seroprevalence. The study population was made up of an initial flock of 197 Spanish Assaf sheep kept in permanent housing using an intensive milking system, and the subsequent five generations of offspring. The control strategy used in this study was mainly based on separating the offspring from the dams after natural intake of colostrum using artificial rearing with milk replacer. Then, replacement groups were formed by these lambs, which were housed in the same shed as the rest of the flock, but in addition, other control measure applied in this study was the isolation of the replacement group from the adults by a barrier (1.20 m high metal sheet) that prevented physical contact between them in the housing, although airspace was shared. Then, replacement groups did not join the adult flock until the onset of first lactation (around 12 months-old). Control measures were first established in 2006 with the second generation offspring, leaving the first replacement group as the control group. Serum samples from all sheep in the flock were screened every six months for antibodies against Maedi-Visna virus using an immunoenzymatic assay. After the application of this control programme, seroprevalence had declined significantly from 93.1% to 54.2% in four years. Results from this study indicate that these control measures may offer a simple alternative for reducing high levels of prevalence in these dairy sheep flocks. Thus, this control strategy is proposed as the first control measures to be taken in flocks with a high prevalence of infection where no other measures could be reasonably applied, followed and expanded by other effective methods when the seroprevalence status of the flock permitting.
    Small Ruminant Research 05/2013; 112(1-3):224-229. DOI:10.1016/j.smallrumres.2012.12.010 · 1.13 Impact Factor
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    ABSTRACT: Mycobacterium avium subsp paratuberculosis (Map) is assumed to infect young ruminants; however, little is known concerning the possibility of adult animals becoming infected. An experimental infection was conducted to establish the effect of age and doses of Map on susceptibility to paratuberculosis in sheep. Sixteen of twenty-four 1.5-month-old Churra lambs and 23 of 30 adult ewes (from 2-11 years old) were orally challenged with an ovine field strain of Map. Thirteen ewes and 8 lambs were infected with a high dose (HD) and 10 adult sheep and 8 lambs with a low dose (LD) of Map. The remaining animals were unchallenged controls. Animals were euthanized at 110 to 120 and 210 to 220 days postinfection. Histological, bacteriological, and nested polymerase chain reaction (PCR) studies were conducted in samples of intestine and related lymphoid tissue (Peyer patches, lymph nodes). Animals were classified according to their lesions. The number of granulomas was counted in 3 tissue sections from each sample. Only the HD groups showed lesions associated with paratuberculosis (92.3% of ewes and 100% of lambs). Adults had lesions characterized by few small demarcated focal granulomas restricted to the lymphoid tissue, whereas granulomas were more numerous and larger, appearing in the lamina propria unrelated to lymphoid tissue, in the lambs. Only HD-infected lambs were positive to culture, whereas nested PCR also detected positive HD ewes and some LD animals. These results suggest that adult sheep can become infected by Map, as seen by the development of lesions, but they are focal and restricted to the lymphoid tissue.
    Veterinary Pathology 02/2013; 50(5). DOI:10.1177/0300985813476066 · 1.87 Impact Factor
  • L. Delgado · L.A. Guilloteau · B. Foret · M.C. Ferreras · J.F. García Marín · V. Pérez
    Journal of Comparative Pathology 01/2013; 148(1):80. DOI:10.1016/j.jcpa.2012.11.136 · 1.14 Impact Factor
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    Journal of Comparative Pathology 01/2013; 148(1):57. DOI:10.1016/j.jcpa.2012.11.046 · 1.14 Impact Factor
  • Journal of Comparative Pathology 01/2013; 148(1). DOI:10.1016/j.jcpa.2012.11.137 · 1.14 Impact Factor
  • Journal of Comparative Pathology 01/2013; 148(1):80. DOI:10.1016/j.jcpa.2012.11.139 · 1.14 Impact Factor
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    ABSTRACT: We examined the distribution in the perivascular spaces of Visna/maedi antigen, T cells (CD3+, CD4+ and CD8+), B cells and macrophages by immunohistochemistry in 22 natural cases of Visna/maedi encephalitis. Sheep showed lymphocytic or histiocytic lesions. In mild lymphocytic lesions, the viral antigen was detected in perivascular cuffs where CD8+ T cells predominated, but in severe lymphocytic lesions, sparse antigen was identified, and CD8+/CD4+ T cells appeared in a similar proportion in multilayer perivascular sleeves. In histiocytic lesions, vessels were surrounded by macrophages with abundant viral antigen, with CD8+/CD4+ T cells and B cells in the periphery. These results could reflect different stages of virus neuroinvasion and clarify the neuropathogenesis of Visna/maedi encephalitis.
    Journal of NeuroVirology 10/2012; 18(6). DOI:10.1007/s13365-012-0131-0 · 2.60 Impact Factor
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    ABSTRACT: Although louping ill affects mainly sheep, a 2011 outbreak in northern Spain occurred among goats. Histopathologic lesions and molecular genetics identified a new strain of louping ill virus, 94% identical to the strain from Britain. Surveillance is needed to minimize risk to domestic and wildlife species and humans.
    Emerging Infectious Diseases 06/2012; 18(6):976-8. DOI:10.3201/eid1806.120220 · 6.75 Impact Factor

Publication Stats

1k Citations
101.33 Total Impact Points


  • 1997–2015
    • Universidad de León
      • • Department of Animal Health
      • • Faculty of Veterinary
      León, Castille and León, Spain
  • 2014
    • Instituto de Ganaderia de Montaña
      León, Castille and León, Spain
  • 2009
    • Saint Leo University
      Florida, United States
  • 2003
    • Servicio Regional de Investigación y Desarrollo Agroalimentario
      Asturias, Spain
  • 1991–1996
    • University of Zaragoza
      • • Department of Pathological Anatomy, Legal Medicine, Toxicology and Health Legislation
      • • Patología Animal
      Caesaraugusta, Aragon, Spain