[show abstract][hide abstract] ABSTRACT: Aim: To evaluate the protective effect of selenium on intestinal ischemia-reperfusion injury due to intussusception. Materials and methods: Forty Wistar albino rats were allocated to 4 equal groups (n = 10). After anesthetization, 2 cm of intestinal segment, 20 cm proximal to the ileocecal valve, was removed in the control group (CG). In the intussusception group (ING), intussusception was performed 20 cm proximal to the ileocecal valve by pushing the proximal intestinal segment distally with a stylet, and 2 cm of intestinal segment was removed 4 h later. Intussusception was reduced and a 2 cm segment was removed after another 4 h period in the intussusception-reduction group (IRG). The selenium group (SG) received 0.2 mg kg -1 of selenium in 10 mL of 0.9% NaCl solution intraperitoneally 20 min before reduction and segments were removed 4 h thereafter. Intestinal tissues were evaluated for histopathological grades and oxidative injury markers. Results: Histopathologic evaluations among the 4 groups did not reveal a significant difference (P > 0.05). Although catalase activity was decreased in all groups, increased activity was found in SG compared with IRG (P < 0.05). GSH-Px and SOD activities were also found to have decreased in ING and IRG (P < 0.05). CG and SG had no difference in GSH-Px and SOD activities (P > 0.05). Malondialdehyde (MDA) activities did not differ between CG and SG, but the MDA level was increased in ING and IRG when compared with CG (P < 0.05). Conclusion: Although selenium prevents oxidative injury by increasing the antioxidant enzyme activity in experimental intussusception, similar effects on histopathologic findings were not detected. Sıçanlarda invajinasyona bağlı intestinal iskemi reperfüzyon hasarında selenyumun koruyucu etkisi Amaç: İnvajinasyona bağlı iskemi-peferfüzyon hasarında selenyumun koruyucu etkisini değerlendirmek üzere deneysel bir çalışma yapılmıştır. Yöntem ve gereç: Kırk Wistar albino sıçan dört gruba ayrılmıştır (n = 10). Anestezi sonrası, kontrol grubunda (KG) ileoçekal valvden 20 cm proksimaldeki 2 cm'lik intestinal segment çıkarılmıştır. İnvajinasyon grubu (İG) invajinasyonun ileoçekal valvden 20 cm proksimalde bir stile yardımı ile proksimal intestinal segmentin distale itilmesi ile oluşturulmuştur. Dört saat sonrasında 2 cm'lik intestinal segment çıkarılmıştır. İnvajinasyon-redüksiyon grubunda (IRG) invajinasyon redükte edildikten 4 saat sonra 2 cm'lik segment çıkartılmıştır. Selenyum grubu (SG) 10 mL % 0,9'luk NaCl solusyonu içerisinde 0,2 mg kg -1 selenium redüksiyondan 20 dakika once intraperitoneal olarak verilerek oluşturulmuş ve 4 saat sonrasında intestinal segment çıkarılmıştır. İntestinal yapılar histopatolojik evrelendirme ve oksidatif hasar belirteçleri bakımından değerlendirilmiştir.
[show abstract][hide abstract] ABSTRACT: We aimed to detect the protective effect of trapidil in ischemia-reperfusion (IR) injury due to ovarian torsion and detorsion. Thirty-two pubertal New Zealand albino rabbits were used. Adnexal torsion was created by rotating the left adnexa including the tubal and ovarian vessels in a 360 degrees clockwise direction. Adnexal detorsion was done by untwisting the adnexa. In the IR group, left oopherectomy was performed after 3 h of adnexal torsion and 3 h of adnexal detorsion. In the study group, a 3-h adnexal torsion was performed and trapidil was administered intraperitoneally as a single dose of 40 mg/kg, 1 h before detorsion. The left oopherectomy was performed after a 3-h adnexal detorsion. In the sham group, sham operation was performed followed by left oopherectomy. In the control group, normal ovarian tissue was evaluated. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels of ovarian tissue were determined for each group. The values of SOD and GSH-Px activities in the IR group were significantly decreased (P < 0.05). In addition, the MDA level was significantly higher in the IR group (P < 0.01). The trapidil-administered group showed significant increase in the levels of GSH-Px (P < 0.05), catalase (P < 0.05), SOD (P < 0.05), and decreased MDA levels (P < 0.05) compared to those in the IR group. The study has shown that trapidil treatment prevents ischemia induced oxidative damage in the ovarian tissues of rabbits.
Pediatric Surgery International 03/2008; 24(3):315-8. · 1.22 Impact Factor
[show abstract][hide abstract] ABSTRACT: The aim of this study is to determine the in vitro sensitivity of mouse esophagus to contracting and relaxing agonists in different pH medium values.
Forty-eight Swiss albino mice (30-40 g) of both sexes were anesthetized with tiopental sodium (30 mg/kg). After exsanguinations from abdominal artery, esophagi were removed and suspended under 0.6 g of resting tension in a tissue bath containing 10 mL of Krebs solution at 37 degrees C. The experiments were performed in different pH mediums 7.4, 6.4, 4, and 2. Carbachol and acetylcholine were used as contractile agonists, and noradrenalin and isoproterenol to evaluate relaxation responses. Data concerning similar concentrations of contractile agonists obtained from different pH mediums were analyzed using Kruskal-Wallis nonparametric analysis of variance and post hoc Dunn test. Relaxation responses were compared with Student t test. A P value less than .05 was considered significant. The study was approved by Local Ethical Committee of Kirikkale University.
Carbachol and acetylcholine caused concentration-dependent contractility in pH 7.4, 6.4, and 4, but contractile responses were inhibited in pH 2. In carbachol and acetylcholine experiments, there was a significant decrease in contractile responses to all concentrations in conjunction with a decreased in pH value. Relaxation responses in pH 2 and 4 could not be obtained because precontraction of tissues was not possible. Noradrenalin and isoproterenol produced concentration-dependent relaxations in pH 7.4 and 6.4. Although noradrenalin responses showed no significant difference according to pH, isoproterenol caused better relaxations in pH 6.4 (between 10(-8) and 10(-6) mol/L) when compared to pH 7.4 studies.
The mouse esophagus has impaired contractile responses to carbachol and acetylcholine in decreased pH values. Contraction responses did not occur in pH medium of 2. In contrast, esophagus segments showed better relaxations in lower pH values with isoproterenol.
Journal of Pediatric Surgery 01/2008; 42(12):1988-92. · 1.38 Impact Factor
[show abstract][hide abstract] ABSTRACT: The aim of the study was the electrophysiological evaluation of the cremasteric reflex after experimental testicular torsion.
Ten male Wistar rats were enrolled into the study. Genitofemoral nerve (GFN) motor conduction and cremasteric reflex (CR) responses were evaluated electrophysiologically after being subjected to anesthesia with intramuscular ketamin hydrochloride. Testicular torsion was performed by rotating the right testicle 720 degrees in a clockwise direction from a midscrotal incision. Electrophysiological evaluations were repeated in the early (30 minutes) and late (90 minutes) periods of testicular torsion. Subsequently, detorsion of the testicles was performed and electrophysiological recordings were completed after 60 minutes of detorsion. The CR was also evaluated clinically before each electrophysiological evaluation. The latency and duration of GFN motor conduction and CR responses was compared for base, early torsion, late torsion and detorsion recordings. Friedman's test for repeated measurements was used for statistical analysis.
The CR, which was detected clinically before torsion and after detorsion, was not detected during torsion. When base, early torsion, late torsion and detorsion recordings were compared, there was no statistical difference with respect to both latency and duration of GFN motor conduction and CR responses (p > 0.05).
Although CR was not detected clinically during testicular torsion, the electrophysiological parameters of the reflex did not differ in the early and late periods of torsion in rats. The GFN motor conduction parameters also showed no differences. In conclusion, the absence of the CR after testicular torsion could not be confirmed by electrophysiological studies.
European Journal of Pediatric Surgery 08/2007; 17(4):261-5. · 0.84 Impact Factor
[show abstract][hide abstract] ABSTRACT: The VACTERL-H syndrome is a rare combination of vertebral anomalies, anal atresia, congenital heart defects, tracheo-esophageal fistula, abnormalities of kidneys and limb anomalies together with hydrocephalus. This condition is recognized as a hereditary entity with poor prognosis. We present a newborn weighing 3400 g, born by cesarean section to a 27 years old mother who had had an irregular antenatal follow-up. The patient had severe hydrocephalus, proximal esophageal atresia and distal tracheoesophageal fistula, gastric outlet obstruction, imperforated anus and recto-urethral fistula, patent ductus arterious, a bifid scrotum, a vertebral defect, sacral dimple and central hypothyroidism. The patient had no limb defects. The association of central hypothyroidism and VACTERL-H has previously not been reported.
[show abstract][hide abstract] ABSTRACT: To investigate the effect of catheterization on urethral wound healing by measuring OH-proline.
Eighteen male New Zealand albino rabbits were divided equally into three groups. In the control group, urethral tissue samples were taken from the ventral penile surface. In group II, the ventral penile surface was incised and primarily repaired by 6/0 polydioxanone. In group III, the incised urethra was repaired by 6/0 polydioxanone and a urethral catheter was inserted. Tissue samples were taken on the 20th postoperative day in groups II and III. Tissue OH-proline levels were measured by the method of Bergman and Loxley. The Kruskal-Wallis statistical test was used for evaluation.
OH-proline values as microg/mg dry tissue were 16.43+/-2.68 in the control group, 14.47+/-1.82 in group II and 14.63+/-0.63 in group III. There was no significant difference between the three groups (P>0.05).
Use of a urethral catheter does not appear to have a direct effect on collagen synthesis in wound healing.
Journal of pediatric urology 06/2006; 2(3):182-4. · 1.38 Impact Factor
[show abstract][hide abstract] ABSTRACT: To show the effect of botulinum A toxin-induced paralysis of abdominal muscles on intraabdominal pressure.
Fifteen Sprague-Dawley rats were divided into 2 groups. An abdominal skin incision was done, and 2 catheters were placed for the pressure monitoring and saline infusion. Saline solution was given to the abdomen until reaching to a pressure level of 9 cm H2O and 6 mm Hg in pressure device, and the amounts of injected saline were recorded. Then intraabdominal saline was drained. Two milliliters (5 U/mL) botulinum A toxin was applied to the abdominal muscles in group 2. Saline was injected at the same points in same amounts in group 1. After 3 days, catheters were placed, and the saline volumes needed to obtain the same pressure levels were recorded for each rat. Spontaneous motor unit potential (MUP), single MUP analysis and interference patterns of the muscles, respiratory rates, and vascular pressure measurements were recorded before and after botulinum toxin (Botox) injections.
Mean intraabdominal saline volumes in the first and third days were 63.8 and 64.4 mL in group 1 and 67.6 and 80.6 mL in group 2, respectively. Mean MUP amplitude and duration of the rectus muscles in group 2 (17.1 microV and 1.47 milliseconds) were significantly lower than those of group 1 (187 microV and 4.9 milliseconds) in the third day. There were no pathological changes in respiratory rates and pressure measurements before and after Botox injections.
This pilot experimental study showed that local injection of botulinum A toxin causes paralysis in abdominal wall muscles, increases the intraabdominal volume, and decreases the pressure, and this application may be used as an adjunct in abdominal wall closure in selective cases.
Journal of Pediatric Surgery 05/2006; 41(4):821-5. · 1.38 Impact Factor
[show abstract][hide abstract] ABSTRACT: We aimed to detect the preventive effects of trapidil in ischemia-reperfusion (IR) injury due to testicular torsion and detorsion.
Forty prepubertal albino rats were used. In the IR group, torsion was created by rotating the left testis over 2 h, and detorsion was done by untwisting the testis. Bilateral orchiectomies were performed after 4 h. In study group, 2-h torsion was performed and trapidil was administered as a single dose 1 h before detorsion. Bilateral orchiectomies were performed after 4 h. In the sham group, a sham operation was done. In the sham plus trapidil group, a sham operation was done and trapidil was administered as a single dose. Testicular tissue malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl (T-SH) levels were determined for each group. The grades of interstitial injury were determined in histopathologic examination.
The NO and MDA levels in the IR group were significantly higher than the study, sham and sham plus trapidil groups in the left testis (P<0.05, P<0.001 and P<0.001, respectively). A statistical difference was not found among study, sham and sham plus trapidil groups in the left testis in NO and MDA levels (P>0.05). The T-SH level in the study group was significantly higher than in the IR, sham and sham plus trapidil groups in left testis P<0.05). In the IR group (left testis), grade 1 interstitial injury was 30% (3/10), grade 2 injury was 60% (6/10) and grade 3 injury was 10% (1/10). In the study group (left testis), grade 1 interstitial injury was 30% (3/10) and there was no injury in 70% (7/10).
Trapidil decreased free oxygen radical formation in testicular torsion and detorsion, and attenuated histopathological damage in the ipsilateral twisted testis.
International Journal of Urology 05/2006; 13(5):601-5. · 1.73 Impact Factor
[show abstract][hide abstract] ABSTRACT: Urethral duplications are rare congenital anomalies. Approximately 200 cases of urethral duplications have been reported in literature. We present a seven-year-old-boy with an embryologic preputial sinus remnant which was mimicking urethral duplication. The clinical presentations and embryology of the urethral duplication and preputial sinus are discussed.
The Indian Journal of Pediatrics 04/2006; 73(3):227-8. · 0.72 Impact Factor
[show abstract][hide abstract] ABSTRACT: Epidermoid cyst is a rare and non-teratomatous, benign tumour of the testis. Testis-sparing surgery is recommended as surgical treatment. We present a 9-year-old-boy with testicular epidermoid cyst. The embryology, histogenesis and treatment of epidermoid cyst of testis, are discussed.
Journal of Indian Association of Pediatric Surgeons. 01/2006;
[show abstract][hide abstract] ABSTRACT: Corrosive esophageal burn is a common health problem in the pediatric age group and causes serious esophageal injuries. The medical treatment in acute phase of corrosive esophageal injury is of particular importance for prevention of esophageal stricture. We therefore aimed to investigate the possible beneficial effect of trapidil (triazolopyrimidine), an inhibitor for phosphodiesterase and platelet-derived-growth-factor, during acute phase of esophageal corrosive injury. Wistar albino rats were randomly allocated to untreated, treated, and sham-operated groups (n = 10 for each group). Corrosive esophageal burn was generated with 10% NaOH solution. The rats were left untreated (untreated group) or treated with trapidil as a single dose of 40 mg/kg intraperitoneally after one hour of the injury (treated group). Abdominal esophageal segment was isolated and tied in sham-control group. The studied esophageal segment was removed from each animal after 24 hours. Malondialdehyde (MDA) and nitric oxide (NO) levels were measured in the esophageal tissues. The ulcer depth was graded by histopathologic examination. MDA and NO levels were significantly higher in the untreated group than in the treated group. Namely, trapidil treatment significantly decreased MDA and NO levels in the injured tissues, the levels of which are similar to those in the tissues of control animals. The grades of ulcer depth were significantly improved in the treated group. These results indicate that the reactive oxygen radicals increase in the early phase of corrosive esophagitis and cause tissue damage. We suggest that trapidil treatment may be useful in acute phase of corrosive esophageal injury.
The Tohoku Journal of Experimental Medicine 12/2005; 207(3):203-8. · 1.37 Impact Factor
[show abstract][hide abstract] ABSTRACT: We present a case of 12-year-old boy with idiopathic benign bilateral testicular enlargement. We eliminated precocious puberty, juvenile hypothyroidism, adrenal rest tumors, X-linked mental retardation, and bilateral testicular neoplasms. The clinical and laboratory features and differential diagnosis of benign bilateral testicular enlargement are discussed.
Journal of Pediatric Surgery 12/2005; 40(11):e23-5. · 1.38 Impact Factor
[show abstract][hide abstract] ABSTRACT: We aimed to investigate the protective effects of trapidil after the occlusion of abdominal aorta and the reperfusion injury in lung. Eighteen New Zealand albino rabbits were used in the study. In six animals [group 1, ischemia-reperfusion (IR) group], the abdominal aorta was exposed and a microvascular clamp was placed in the infrarenal abdominal aorta for 60 min. After the ischemic period, the microvascular clamp was removed and reperfusion was provided for 2 h. After the reperfusion period, the lungs were removed carefully and specimens were prepared for histopathological and biochemical studies in appropriate conditions. In group 2 (study group), trapidil (Rocarnal, Rentschler-UCB GmbH, Kerpen, Germany) was administered intraperitoneally as a single dose 1 h prior to trial, the IR procedure was performed and lung specimens were prepared similar to group 1. In group 3 (sham group), the infrarenal abdominal aorta was exposed and lung specimens were prepared for histopathological and biochemical studies at the end of the study. Histopathological changes, malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl group (T-SH) levels were evaluated. There was a statistical difference between the IR group and study group regarding NO and MDA levels (P < 0.05 and P < 0.01, respectively), but this was not detected between the IR group and the sham group (P > 0.05). There was no statistical difference among the three groups regarding T-SH levels (P > 0.05). While a statistical difference was found between the sham group and study group in the NO level (P < 0.05), no statistical difference was found in the MDA level (P > 0.05). There was a statistical difference in interstitial edema, PMN infiltration and hemorrhage scores among the groups (P < 0.05). There was a statistical difference between the IR group and study group in PMN infiltration (P < 0.05), but this was not detected between the groups in interstitial edema and hemorrhage scores (P > 0.05). There was a statistical difference between IR group and sham group in interstitial edema, PMN infiltration and hemorrhage scores (P < 0.05). Statistical difference was found between the sham group and study group in interstitial edema and hemorrhage scores (P < 0.05), but not in PMN infiltration (P > 0.05). Conclusions: Infrarenal abdominal aortic occlusion and reperfusion causes lung injury. We conclude that trapidil has preventive effects in the lung tissue after IR injury.
Pediatric Surgery International 12/2005; 21(12):983-8. · 1.22 Impact Factor
[show abstract][hide abstract] ABSTRACT: This study investigated the factors influencing the effectiveness of 7.5% polyvidone iodine as a surgical antiseptic. The study involved 100 operating staff (75 doctors and 25 nurses) from hospital surgical teams. Fingertips of both hands of the subjects were pressed on to agar culture before and after washing and after completion of surgery. Handwashing lasting for more than 3 min led to a significant decrease in the number of colonies compared with handwashing lasting for less than 3 min. Moreover, the number of colonies was significantly higher when surgery lasted for longer than 95 min. However, the handwashing style (with or without brushing) was not found to have a significant effect on the outcome of the disinfection procedure in terms of bacterial colonization. Subjects who had colonization of their hands after surgery were found to have significantly higher colony counts before handwashing compared with those who did not have any colonization on their hands after surgery. The results of this study revealed that in order to attain effective disinfection with polyvidone iodine, the duration of handwashing should be at least 3 min. The risk of recolonization increases when the duration of surgery exceeds 95 min.
Journal of Hospital Infection 10/2005; 61(1):15-9. · 2.86 Impact Factor
[show abstract][hide abstract] ABSTRACT: One of the factors suggested to be causative in hernia formation is defective collagen metabolism. In this study, we investigated whether there is any relationship between the collagen contents of indirect hernial sacs and certain demographic and anatomic parameters.
Hernial sac hydroxyproline levels were measured in 100 pediatric and 23 adult indirect inguinal hernia cases as indicators of collagen concentrations. Hydroxyproline levels were compared between boys and girls, right and left sided hernias, unilateral and bilateral cases, children and adults. Whether there was any relationship between patient age and hydroxyproline level was also investigated.
No difference was found between males and females (p > 0.05), right and left sided hernias (p > 0.05) or unilateral and bilateral cases (p > 0.05). Hydroxyproline levels of adults were found to be significantly higher than those of children (p = 0.000). There was also a positive correlation between age and hydroxyproline levels of boys (r = 0.285, p = 0.023).
Although a relationship between direct inguinal hernia and collagen structure has been shown by some investigators, we were unable to point to any significant findings which would indicate such a relationship in indirect inguinal hernia cases. Further studies on hydroxyproline levels in peritoneal samples will be necessary to understand the role of peritoneal hydroxyproline levels in hernia formation.
European Journal of Pediatric Surgery 09/2005; 15(4):258-61. · 0.84 Impact Factor
[show abstract][hide abstract] ABSTRACT: The aim of the study was to investigate revascularization of the testicle through its own tissue and spermatic vessels by a bladder pedicle flap in rabbits.
Thirty male rabbits were used in the study. In 10 animals, the Fowler-Stephens (FS) procedure was applied to the right testicles (FS group). The FS procedure and revascularization of the testicle by a bladder pedicle flap were applied to the right testicles in 10 animals (FSO group). No surgical procedure was performed in the control group. Scintigraphic study was performed 3 weeks after the high ligation procedure in the FS and FSO groups. Both testicles were evaluated by radionuclide scintigraphy in all the animals. Macroscopic testicle weights were evaluated. Testicular biopsy scores and mean seminiferous tubule diameters were determined in the histopathologic study.
The testicular blood flow of the FSO group was better than the FS group (P < .05), but there was no statistically significant difference between the FSO and the control groups (P > .01). Testicle weights, testicular biopsy scores, and seminiferous tubule diameters in the FSO group were detected, and there was a statistically significant difference when compared with the FS group (P < .05) but not with the control group (P > .01).
We suppose that the surgical model of revascularization of the testicle through its own tissue and spermatic vessels by a bladder pedicle flap can be an alternative to the FS procedure in abdominal and high canalicular undescended testicles.
Journal of Pediatric Surgery 08/2005; 40(8):1295-300. · 1.38 Impact Factor
[show abstract][hide abstract] ABSTRACT: Duplications of the alimentary tract are rare congenital malformations and may occur anywhere in the intestinal tract. Intestinal duplication cysts have been rarely found with intestinal malrotation anomaly. We present a 2-year old boy who had intestinal duplication cyst, associated with intestinal malrotation anomaly.