Yoshinobu Goto

International University of Health and Welfare, Otahara, Tochigi, Japan

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Publications (96)210.84 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Proliferative vitreoretinopathy (PVR) is a severe, vision-threatening disorder characterized by the fibrous membrane formation that leads to tractional retinal detachment. There has been no effective therapeutic approach other than vitreoretinal surgery. In this study, DNA microarray analysis of the fibrous membranes revealed significant up-regulation of periostin. We also found increased periostin expression in the vitreous and retinal pigment epithelial (RPE) cells from fibrous membranes of PVR patients. In vitro, periostin increased proliferation, adhesion, migration, and collagen production in RPE cells through integrin αV-mediated FAK and AKT phosphorylation. Periostin blockade suppressed migration and adhesion induced by TGFβ2 and PVR vitreous. In vivo, periostin inhibition had the inhibitory effect on progression of experimental PVR in rabbit eyes without affecting the viability of retinal cells. These results identified periostin as a pivotal molecule for fibrous membrane formation as well as a promising therapeutic target for PVR.-Ishikawa, K., Yoshida, S., Nakao, S., Nakama, T., Kita, T., Asato, R., Sassa, Y., Arita, R., Miyazaki, M., Enaida, H., Oshima, Y., Murakami, N., Niiro, H., Ono, J., Matsuda, A., Goto, Y., Akashi, K., Izuhara, K., Kudo, A., Kono, T., Hafezi-Moghadam, A., Ishibashi, T. Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy.
    The FASEB Journal 09/2013; · 5.70 Impact Factor
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    ABSTRACT: Visual evoked potentials (VEPs) are the electrical responses from the brain concerned with visual information processing. Amplitude of VEPs is smaller than that of background EEG activity, and the stimulus-locked averaging method is usually used for obtained the waveform. VEP response to each stimulus is not completely the same however it is varying with its amplitude and duration. Therefore, amplitude of averaged VEP waveform deteriorates due to their variability in raw data. Feature extraction of background EEG activity during visual stimulation is also a one of significant items in VEP analysis. In that case, separation of VEP component and background EEG component (mainly posterior dominant rhythm) is crucial. In the past, we proposed the method of estimating both amplitude of VEP and dominant rhythm by use of EEG model. This present study, the proposed method was applied to actual recorded VEP data and its effectiveness was evaluated. EEGs with visual stimulus were recorded from nine healthy young adults. Usefulness of the proposed method was investigated by comparing the conventional power spectrum averaging method. The proposed method will be applicable to show an accurate VEP analysis and characteristic analysis of background activity under visual stimulus.
    Control, Automation and Systems (ICCAS), 2013 13th International Conference on; 01/2013
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    ABSTRACT: Visual evoked potential (VEP) is widely used for diagnosis and analysis of the brain function on visual system. Accuracy of the analysis depends on the quality of VEP record, so the medical doctors and/or medical technologists always pay attention to the recording state during the recording. In this study, the real-time evaluation system for VEP recording was newly developed. The system can show the following information in real-time; characteristic parameters of VEP, contaminated artifacts affecting VEP recording and background EEG activity. Characteristic parameters of VEP are also possible to compare with their past tendency obtained from data base. Blink artifacts, EMG artifacts and posterior alpha wave were detected for evaluating the state of recording. Developed system was applied to VEP recording of 10 healthy adults. Effectiveness of the proposed system was investigated.
    Complex Medical Engineering (CME), 2012 ICME International Conference on; 01/2012
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    ABSTRACT: In humans, motion information is mainly processed by the dorsal visual stream. This stream consists of two functional streams: the ventro-dorsal (v-d) and dorso-dorsal (d-d) streams. Patients with Alzheimer's disease (AD) and mild cognitive impairment (MCI) exhibit an impairment in motion perception. By using visual event-related potentials (ERPs), we have previously demonstrated that v-d function related to optic flow (OF) perception is selectively impaired in the dorsal stream in MCI patients. The present study is aimed at examining the difference in the changes in two functional dorsal streams among MCI and AD patients and healthy controls. Therefore, we recorded ERPs in response to OF and horizontal (HO) motion stimuli in patients with AD and MCI, and healthy controls. In all groups, motion-related N170 (V5/middle temporal (MT) origin) and OF-specific P200 (v-d origin) were elicited as major components. MCI patients showed a prolonged latency of P200 for OF but not of N170 for both stimuli compared with healthy controls. In contrast, the latencies of N170 for both stimuli and P200 for OF were significantly prolonged in AD patients compared with other groups. These findings indicate that MCI patients exhibit a selective impairment in the v-d stream, whereas AD patients have impairments in distributed dorsal stream function. These ERP results may reflect the progression of pathological changes in the course of the disease. Therefore, motion-related ERPs are useful to detect and track changes in the brain function of patients with MCI and AD.
    Complex Medical Engineering (CME), 2012 ICME International Conference on; 01/2012
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    ABSTRACT: Alzheimer's disease (AD) patients have visuospatial deficits due to parietal dorsal stream dysfunction. Two distinct dorsal flows have been proposed: the inferior parietal (ventro-dorsal (v-d)) and superior parietal (dorso-dorsal (d-d)) streams. We aimed to elucidate how the two dorsal streams are altered in patients with amnestic mild cognitive impairment (aMCI) and AD. Thus, the psychophysical threshold measurements and visual event-related potentials (ERPs) were recorded in patients with aMCI and AD, and in healthy old and young adults. The visual stimuli included radial optic flow (OF) derived from the v-d stream and horizontal (HO) motion conveyed from the d-d stream. The motion thresholds between aMCI patients and old adults were comparable. However, AD patients showed significantly higher motion thresholds for both stimuli compared with other groups. In lower-level ERPs, there were no significant differences in P1 (100 ms) and N1 (130 ms) for both stimuli among the groups. For higher-level ERPs, aMCI patients showed the prolonged latency of OF-specific P200 (v-d origin) and comparable latency of motion-related N170 (V5/MT origin) for both stimuli compared with old adults. In AD patients, both N170 and P200 latencies were significantly prolonged compared with other groups. P200 latency was closely correlated with the Mini-Mental State Examination score. These findings indicate that the v-d function related to OF perception is selectively impaired in aMCI, whereas AD has impairment of the distributed higher-level dorsal stream. Therefore, OF-specific P200 can be useful for detecting early functional changes of the brain in aMCI.
    Journal of Alzheimer's disease: JAD 11/2011; 28(3):695-708. · 4.17 Impact Factor
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    ABSTRACT: People with autism spectrum disorder (ASD) often show inferior global motion performance with superior performance in detail form perception, suggesting dysfunction of the dorsal visual stream. To elucidate the neural basis of impaired global motion perception in ASD, we measured psychophysical threshold and visual event-related potentials (ERPs) with a 128-channel system in 12 ASD and 12 healthy control adults. Radial optic flow (OF) and horizontal motion (HO) were used as the visual stimuli. The former was related to the ventro-dorsal stream formed by the inferior parietal lobule, while the latter was conveyed from the dorso-dorsal stream formed by the superior parietal lobule. No significant group differences were observed in the motion thresholds for both OF and HO. N170 and P200 were elicited as major components of ERPs in both groups. However, the latencies of both components for OF but not HO were significantly prolonged in ASD compared with the control group. Our ERP results suggest that ASD has a selective impairment for OF processing even though the psychophysical thresholds are preserved. Therefore, we provide the first electrophysiological evidence for altered function of the higher-level dorsal visual stream in ASD, specifically the ventro-dorsal stream closely related to OF perception.
    Research in Autism Spectrum Disorders 01/2011; 5(1):400-407. · 2.96 Impact Factor
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    ABSTRACT: distribution of vi­ sual evoked potentials (VEPs) in electroencephalo graphic (EEG) record brings about the significant information for visual pro­ cessing in human brain. This study proposes a method for estimating topographical distribution of YEP from the separated power spectrum components by combining the models. VEPs with various temporal frequencies were recorded from nine healthy adults. Original power spectrum consisting of YEP, background activities, artifacts and other components were then obtained. To the extract the YEP components from the original power spectrum, models corresponding to background activities especially for posterior dominant alpha rhythm, low frequency component and high frequency component caused from electromyographi c (EMG) artifact were constructed and those parameters were estimated. Finally, YEP components were calculated by subtracting those components from the original power spectrum. Topographical distribution of both first har­ monic (IF) and second harmonic (2F) components of YEP were obtained by the proposed method. Estimated other components except VEPs were also investigated. Merits and usefulness of the proposed method were analyzed by comparing the conventional stimulus-locked averaging method in the time domain. Proposed method has several advanced points for conventional averaging method. Particularly, posterior dominant alpha rhythm and EMG artifact were directly taken into account in the YEP components estimation. Therefore, accurate estimation of YEP components can be done even if those components were contaminated with raw EEG. To obtain the topographical distribution of IF and 2F components respectively is also possible. Index Terms-Visual Evoked Potentials (VEPs), Topographical mapping, Signal separation
    01/2011;
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    ABSTRACT: By constructing Gaussian Naïve Bayes Classifiers, we have re-analyzed data from an earlier event-related potential (ERP) study of an illusion in time perception known as auditory temporal assimilation. In auditory temporal assimilation, two neighboring physically unequal time intervals marked by three successive tone bursts are illusorily perceived as equal if the two time intervals satisfy a certain relationship. The classifiers could discriminate whether or not the subject was engaged in the task, which was judgment of the subjective equality between the two intervals, at an accuracy of >79%, and from principal component scores of individual average ERP waveforms, we were able to predict their subjective judgments to each stimulus at an accuracy of >70%. Chernoff information, unlike accuracy or Kullback–Leibler (KL) distance, suggested brain activation associated with auditory temporal assimilation at an early pre-decision stage. This may provide us with a simple and useful neural decoding scheme in analyzing information processing of temporal patterns in the brain.
    Neural Computing and Applications 01/2011; 20:965-973. · 1.76 Impact Factor
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    ABSTRACT: Lentiviral vectors are promising tools for the treatment of chronic retinal diseases including glaucoma, as they enable stable transgene expression. We examined whether simian immunodeficiency virus (SIV)-based lentiviral vector-mediated retinal gene transfer of human pigment epithelium-derived factor (hPEDF) can rescue rat retinal ganglion cell injury. Gene transfer was achieved through subretinal injection of an SIV vector expressing human PEDF (SIV-hPEDF) into the eyes of 4-week-old Wistar rats. Two weeks after gene transfer, retinal ganglion cells were damaged by transient ocular hypertension stress (110 mmHg, 60 min) and N-methyl-d-aspartic acid (NMDA) intravitreal injection. One week after damage, retrograde labeling with 4',6-diamidino-2-phenylindole (DAPI) was done to count the retinal ganglion cells that survived, and eyes were enucleated and processed for morphometric analysis. Electroretinographic (ERG) assessment was also done. The density of DAPI-positive retinal ganglion cells in retinal flat-mounts was significantly higher in SIV-hPEDF-treated rats compared with control groups, in both transient ocular hypertension and NMDA-induced models. Pattern ERG examination demonstrated higher amplitude in SIV-hPEDF-treated rats, indicating the functional rescue of retinal ganglion cells. These findings show that neuroprotective gene therapy using hPEDF can protect against retinal ganglion cell death, and support the potential feasibility of neuroprotective therapy for intractable glaucoma.
    Human gene therapy 12/2010; 22(5):559-65. · 4.20 Impact Factor
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    ABSTRACT: Event-related potentials (ERPs) were recorded to examine neural responses to face stimuli in a masking paradigm. Images of faces (neutral or fearful) and objects were presented in subthreshold, threshold, and suprathreshold conditions (exposure durations of approximately 20, 30 and 300 ms, respectively), followed by a 1000-ms pattern mask. We recorded ERP responses at Oz, T5, T6, Cz and Pz. The effects of physical stimulus features were examined by inverted stimuli. The occipital N1 amplitude (approximately 160 ms) was significantly smaller in response to faces than objects when presented at a subthreshold duration. In contrast, the occipitotemporal N170 amplitude was significantly greater in the threshold and suprathreshold conditions compared with the subthreshold condition for faces, but not for objects. The P1 amplitude (approximately 120 ms) elicited by upright faces in the subthreshold condition was significantly larger than for inverted faces. P1 and N1 components at Oz were sensitive to subthreshold faces, which suggests the presence of fast face-specific process(es) prior to face-encoding. The N170 reflects the robustness of the face selective response in the occipitotemporal area. Even when presented for a subthreshold duration, faces were processed differently to images of objects at an early stage of visual processing.
    Clinical neurophysiology: official journal of the International Federation of Clinical Neurophysiology 11/2010; 122(4):708-18. · 3.12 Impact Factor
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    ABSTRACT: This study was performed to elucidate whether transcranial direct current stimulation (tDCS) over the motor association cortex modifies the excitability of primary motor (M1) and somatosensory (S1) cortices via neuronal connectivity. Anodal, cathodal, and sham tDCS (1 mA) over the left motor association cortex was applied to 10 subjects for 15 min using electrodes of two sizes (9 and 18 cm(2)). Both motor evoked potentials (MEPs) and somatosensory evoked potentials (SEPs) were recorded before, immediately after, and 15 min after tDCS. Electrode positions were confirmed by overlaying them on MRI anatomical surface images of two individuals. After applying anodal tDCS using the large electrode, amplitudes of MEP components significantly decreased, whereas those of early SEP components (N20 and P25) increase. Opposite effects were observed on MEPs and SEPs after cathodal tDCS. However, a small electrode did not significantly influence either MEPs or SEPs, irrespective of polarity. The small electrode covered mainly the dorsal premotor cortex (PMd) while the large electrode involved the supplementary motor area (SMA) in addition to PMd. These results suggest that anodal tDCS over PMd together with SMA enhanced the inhibitory input to M1 and excitatory input to S1, and that cathodal tDCS might lead to an opposite effect. The finding that only the large electrode modulated M1 and S1 implies that activation of PMd together with SMA by tDCS can induce plastic changes in primary sensorimotor areas.
    Clinical neurophysiology: official journal of the International Federation of Clinical Neurophysiology 11/2010; 122(4):777-83. · 3.12 Impact Factor
  • Clinical Neurophysiology 10/2010; 121. · 3.14 Impact Factor
  • Clinical Neurophysiology 10/2010; 121. · 3.14 Impact Factor
  • Clinical Neurophysiology - CLIN NEUROPHYSIOL. 01/2010; 121.
  • Yoshinobu Goto, Takao Yamasaki, Shozo Tobimatsu
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    ABSTRACT: It is well known that visual evoked potentials (VEPs) are useful for investigating the physiology and pathophysiology of human visual system. VEPs can be used effectively in association with psychophysics to study both normal and abnormal visual functions. Recent progress of VEPs is based on two concepts: 1) visual information is processed simultaneously via multiple parallel pathways or channels and 2) a functional specialization in the visual system exists so that different attributes of the visual scene are processed in an anatomically separate part of the visual cortex. In this study, we first summarize the concepts of the functional anatomy and physiology of human visual system. Then, we emphasize the innovation for the visual stimuli based on the physiological properties of parallel visual pathways.
    01/2010;
  • Clinical Neurophysiology - CLIN NEUROPHYSIOL. 01/2010; 121.
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    ABSTRACT: In an axonal variant of Guillain-Barré syndrome (GBS) associated with Campylobacter jejuni (C. jejuni) enteritis, the mechanism underlying axonal damage is obscure. We purified and characterized a DNA-binding protein from starved cells derived from C. jejuni (C-Dps). This C-Dps protein has significant homology with Helicobacter pylori neutrophil-activating protein (HP-NAP), which is chemotactic for human neutrophils through binding to sulfatide. Because sulfatide is essential for paranodal junction formation and for the maintenance of ion channels on myelinated axons, we examined the in vivo effects of C-Dps. First, we found that C-Dps specifically binds to sulfatide by ELISA and immunostaining of thin-layer chromatograms loaded with various glycolipids. Double immunostaining of peripheral nerves exposed to C-Dps with anti-sulfatide antibody and anti-C-Dps antibody revealed co-localization of them. When C-Dps was injected into rat sciatic nerves, it densely bound to the outermost parts of the myelin sheath and nodes of Ranvier. Injection of C-Dps rapidly induced paranodal myelin detachment and axonal degeneration; this was not seen following injection of PBS or heat-denatured C-Dps. Electron microscopically, C-Dps-injected nerves showed vesiculation of the myelin sheath at the nodes of Ranvier. Nerve conduction studies disclosed a significant reduction in compound muscle action potential amplitudes in C-Dps-injected nerves compared with pre-injection values, but not in PBS-, heat-denatured C-Dps-, or BSA-injected nerves. However, C-Dps did not directly affect Na(+) currents in dissociated hippocampal neurons. Finally, when C-Dps was intrathecally infused into rats, it was deposited in a scattered pattern in the cauda equina, especially in the outer part of the myelin sheath and the nodal region. In C-Dps-infused rats, but not in BSA-infused ones, a decrease in the number of sodium channels, vesiculation of the myelin sheath, axonal degeneration and infiltration of Iba-1-positive macrophages were observed. Thus, we consider that C-Dps damages myelinated nerve fibers, possibly through interference with paranodal sulfatide function, and may contribute to the axonal pathology seen in C. jejuni-related GBS.
    Journal of the neurological sciences 10/2009; 288(1-2):54-62. · 2.32 Impact Factor
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    ABSTRACT: PURPOSE. Our aim was to better understand how to isolate the cone-mediated response in rats. Therefore, we studied the difference of ERGs in the course of light adaptation between 2 and 20 Hz stimulus frequencies. METHODS. A total of 90 rats divided into 18 different groups were used following overnight dark adaptation. ERGs were recorded against 3 different adapting field luminances (1.15, 1.50 or 1.75 log cd/m 2) with a combination of 3 stimulus flash intensities (0.86, 1.30 or 2.03 log cd sec/m 2) . The responses were obtained at 2 minute intervals for 25 minutes of light adaptation. RESULTS. The response of the rat cone ERG was large despite the small number of cones. The mean amplitude increased systematically from the dark-adapted value requiring more than 15 minutes to reach an asymptote at 2 Hz stimulation, but only 10 minutes at 20 Hz stimulation. The 2 Hz adaptation curves had biphasic pattern compared to the monophasic 20 Hz curve. This second increase in the amplitude at 2 Hz appeared at around 7–8 minutes as a function of adaptation time. This tendency was most evident when using a low adapting field luminance with a high flash intensity. CONCLUSIONS. Our results suggest that the rods intrude much more during light adaptation at 2 Hz stimulation in rodents than in humans. Therefore, 20 Hz flicker stimulation can better isolate more the cone-mediated function than 2 Hz stimulation during the course of light adaptation in rats. Furthermore, the functional characteristics of the cone in rats may be different from that in humans.
    Current Eye Research. 07/2009; 19(3).
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    ABSTRACT: A phase 1 clinical trial evaluating the safety of gene therapy for patients with wet age-related macular degeneration (AMD) or retinoblastoma has been completed without problems. The efficacy of gene therapy for Leber's congenital amaurosis (LCA) was reported by three groups. Gene therapy may thus hold promise as a therapeutic method for the treatment of intractable ocular diseases. However, it will first be important to precisely evaluate the efficiency and safety of alternative gene transfer vectors in a preclinical study using large animals. In the present study, we evaluated the acute local (ophthalmic) and systemic toxicity of our simian immunodeficiency virus from African green monkeys (SIVagm)-based lentiviral vectors carrying human pigment epithelium-derived factor (SIV-hPEDF) for transferring genes into nonhuman primate retinas. Transient inflammation and elevation of intraocular pressure were observed in some animals, but these effects were not dose dependent. Electroretinograms (ERGs), including multifocal ERGs, revealed no remarkable change in retinal function. Histopathologically, SIV-hPEDF administration resulted in a certain degree of inflammatory reaction and no apparent structural destruction in retinal tissue. Regarding systemic toxicity, none of the animals died, and none showed any serious side effects during the experimental course. No vector leakage was detected in serum or urine samples. We thus propose that SIVagm-mediated stable gene transfer might be useful and safe for ocular gene transfer in a clinical setting.
    Human gene therapy 06/2009; 20(9):943-54. · 4.20 Impact Factor
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    ABSTRACT: Purpose: Our aim was to study whether transcranial direct current stimulation (tDCS) over premotor cortex (PM) can modify the excitability of the ipsilateral primary motor (M1) and somatosensory (S1) cortices via cortico-cortical connectivity. Methods: Ten subjects received, anodal, cathodal and sham tDCS (1mA) over left PM for 15min. PM was determined to be 2cm anterior and 3cm medial to the hotspot of right first dorsal interosseus muscle. Motor evoked potentials (MEPs) were recorded from right first dorsal interosseus (FDI) muscle with transcranial magnetic stimulation over left M1. Somatosensory evoked potentials (SEPs) to right median nerve stimulation were also recorded from left C3'. Both MEPs and SEPs were recorded before, immediately after and 15min after tDCS. Results: The amplitudes of MEPs after anodal tDCS were shown to decrease while those of SEPs tended to increase. In contrast, the effects of cathodal tDCS were opposite to those of anodal tDCS. Statistical analysis (ANOVA) revealed that a significant interaction among INTERVENTION (anodal, cathodal) times TIME (before, after, after15 min) on both MEPs (p Lt 0.01) and SEPs (p Lt 0.05). Discussion: We infer that decreased MEP amplitudes resulted from inhibitory input to M1 from PM with anodal tDCS over PM, whereas the opposite effect was mediated from PM to M1 with cathodal tDCS. It is likely that changes in S1 excitability reflect the alternation of input-output modulation between M1 and S1. Conclusion: tDCS is useful for modulating the excitability of PM with which plastic functions of M1 and S1 can be assessed.
    Complex Medical Engineering, 2009. CME. ICME International Conference on; 05/2009

Publication Stats

1k Citations
210.84 Total Impact Points

Institutions

  • 2005–2011
    • International University of Health and Welfare
      • Department of Occupational Therapy
      Otahara, Tochigi, Japan
  • 1994–2011
    • Kyushu University
      • • Department of Clinical Neurophysiology
      • • Department of Ophthalmology
      • • Department of Neurology
      • • Department of Neurosurgery
      • • Faculty of Medical Sciences
      Fukuoka-shi, Fukuoka-ken, Japan
  • 2009–2010
    • Niigata University of Health and Welfare
      Niahi-niigata, Niigata, Japan