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Mohammed Sebaihia,
Andrew Preston,
Duncan J Maskell,
Holly Kuzmiak,
Terry D Connell,
Natalie D King,
Paul E Orndorff,
David M Miyamoto,
Nicholas R Thomson,
David Harris,
Arlette Goble,
Angela Lord,
Lee Murphy,
Michael A Quail,
Simon Rutter,
Robert Squares, Steven Squares,
John Woodward,
Julian Parkhill,
Louise M Temple
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ABSTRACT: Bordetella avium is a pathogen of poultry and is phylogenetically distinct from Bordetella bronchiseptica, Bordetella pertussis, and Bordetella parapertussis, which are other species in the Bordetella genus that infect mammals. In order to understand the evolutionary relatedness of Bordetella species and further the understanding of pathogenesis, we obtained the complete genome sequence of B. avium strain 197N, a pathogenic strain that has been extensively studied. With 3,732,255 base pairs of DNA and 3,417 predicted coding sequences, it has the smallest genome and gene complement of the sequenced bordetellae. In this study, the presence or absence of previously reported virulence factors from B. avium was confirmed, and the genetic bases for growth characteristics were elucidated. Over 1,100 genes present in B. avium but not in B. bronchiseptica were identified, and most were predicted to encode surface or secreted proteins that are likely to define an organism adapted to the avian rather than the mammalian respiratory tracts. These include genes coding for the synthesis of a polysaccharide capsule, hemagglutinins, a type I secretion system adjacent to two very large genes for secreted proteins, and unique genes for both lipopolysaccharide and fimbrial biogenesis. Three apparently complete prophages are also present. The BvgAS virulence regulatory system appears to have polymorphisms at a poly(C) tract that is involved in phase variation in other bordetellae. A number of putative iron-regulated outer membrane proteins were predicted from the sequence, and this regulation was confirmed experimentally for five of these.
Journal of Bacteriology 09/2006; 188(16):6002-15. · 3.83 Impact Factor
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William C. Nierman,
Arnab Pain,
Michael J. Anderson,
Jennifer R. Wortman,
H. Stanley Kim,
Javier Arroyo,
Matthew Berriman,
Keietsu Abe,
David B. Archer,
Clara Bermejo, [......],
Owen White,
John Woodward,
Jae-Hyuk Yu,
Claire Fraser,
James E. Galagan,
Kiyoshi Asai,
Masayuki Machida,
Neil Hall,
Bart Barrell,
David W. Denning
Nature 01/2006; 439(7075):502-502. · 36.28 Impact Factor
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William C Nierman,
Arnab Pain,
Michael J Anderson,
Jennifer R Wortman,
H Stanley Kim,
Javier Arroyo,
Matthew Berriman,
Keietsu Abe,
David B Archer,
Clara Bermejo, [......],
Owen White,
John Woodward,
Jae-Hyuk Yu,
Claire Fraser,
James E Galagan,
Kiyoshi Asai,
Masayuki Machida,
Neil Hall,
Bart Barrell,
David W Denning
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ABSTRACT: Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen. Its conidia production is prolific, and so human respiratory tract exposure is almost constant. A. fumigatus is isolated from human habitats and vegetable compost heaps. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.
Nature 01/2006; 438(7071):1151-6. · 36.28 Impact Factor
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Arnab Pain,
Hubert Renauld,
Matthew Berriman,
Lee Murphy,
Corin A Yeats,
William Weir,
Arnaud Kerhornou,
Martin Aslett,
Richard Bishop,
Christiane Bouchier, [......],
Alan R Walker,
John Woodward,
Dirk A E Dobbelaere,
Gordon Langsley,
Marie-Adele Rajandream,
Declan McKeever,
Brian Shiels,
Andrew Tait,
Bart Barrell,
Neil Hall
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ABSTRACT: Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome of T. annulata and compared it with that of T. parva to understand the mechanisms underlying transformation and tropism. Despite high conservation of gene sequences and synteny, the analysis reveals unequally expanded gene families and species-specific genes. We also identify divergent families of putative secreted polypeptides that may reduce immune recognition, candidate regulators of host-cell transformation, and a Theileria-specific protein domain [frequently associated in Theileria (FAINT)] present in a large number of secreted proteins.
Science 08/2005; 309(5731):131-3. · 31.20 Impact Factor
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Julian Parkhill,
Mohammed Sebaihia,
Andrew Preston,
Lee D Murphy,
Nicholas Thomson,
David E Harris,
Matthew T G Holden,
Carol M Churcher,
Stephen D Bentley,
Karen L Mungall, [......],
Sarah Sharp,
Mark Simmonds,
Jason Skelton,
Robert Squares, Steven Squares,
Kim Stevens,
Louise Unwin,
Sally Whitehead,
Bart G Barrell,
Duncan J Maskell
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ABSTRACT: Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica are closely related Gram-negative beta-proteobacteria that colonize the respiratory tracts of mammals. B. pertussis is a strict human pathogen of recent evolutionary origin and is the primary etiologic agent of whooping cough. B. parapertussis can also cause whooping cough, and B. bronchiseptica causes chronic respiratory infections in a wide range of animals. We sequenced the genomes of B. bronchiseptica RB50 (5,338,400 bp; 5,007 predicted genes), B. parapertussis 12822 (4,773,551 bp; 4,404 genes) and B. pertussis Tohama I (4,086,186 bp; 3,816 genes). Our analysis indicates that B. parapertussis and B. pertussis are independent derivatives of B. bronchiseptica-like ancestors. During the evolution of these two host-restricted species there was large-scale gene loss and inactivation; host adaptation seems to be a consequence of loss, not gain, of function, and differences in virulence may be related to loss of regulatory or control functions.
Nature Genetics 10/2003; 35(1):32-40. · 35.53 Impact Factor
