Canio Buonavoglia

Università degli Studi di Bari Aldo Moro, Bari, Apulia, Italy

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Publications (312)718.57 Total impact

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    ABSTRACT: A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2 and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as 10 copies of viral RNA. By real-time RT-PCR analysis of 986 biological samples collected from cattle herd with clinical signs suggestive of pestivirus infection and from animals recruited in a pestivirus surveillance programme, 165 pestivirus positive samples were detected, including 6 specimens, 2 nasal swabs and 4 EDTA-blood samples, that tested negative by a gel-based RT-PCR assay targeting the 5'UTR. The developed TaqMan assay represents a new reliable and effective tool for rapid and sensitive diagnosis of infections caused by all pestiviruses circulating in cattle, thus being useful for extensive surveillance programs in geographic areas where HoBi-like pestiviruses are co-circulating with BVDV-1 and BVDV-2. Copyright © 2015. Published by Elsevier B.V.
    Journal of virological methods 08/2015; DOI:10.1016/j.jviromet.2015.08.013 · 1.88 Impact Factor
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    ABSTRACT: A calicivirus was detected in neonatal calves with enteritis in Kırklareli, Thrace, Turkey. In the full-length genome, Kırklareli virus was more related (48% nucleotide identity) to bovine enteric caliciviruses (Nebovirus genus). The virus was also detected in a herd in Ankara, Central Anatolia, but not in other Turkish prefectures. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
    Journal of clinical microbiology 08/2015; DOI:10.1128/JCM.01736-15 · 4.23 Impact Factor
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    ABSTRACT: Vesiviruses have been detected in several animal species and as accidental contaminants of cells. We detected vesiviruses in asymptomatic kennel dogs (64.8%) and symptomatic (1.1%) and asymptomatic (3.5%) household dogs in Italy. The full-length genome of 1 strain, Bari/212/07/ITA, shared 89%-90% nt identity with vesiviruses previously detected in contaminated cells.
    Emerging Infectious Diseases 08/2015; 21(8):1433-1436. DOI:10.3201/eid2108.140900 · 7.33 Impact Factor
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    ABSTRACT: Canine coronavirus types I (CCoV-I) and II (CCoV-II) are usually responsible for mild enteritis in dogs. While the CCoV-II genome has been completely sequenced, to date there are no complete genomic sequence data available publicly for CCoV-I. Thus, the aim of the present study was to analyse the full-length genome of a CCoV-I prototype strain that had been recovered from a dog with diarrhea in Italy. CCoV-I strain 23/03 has a genome of 30,000 nucleotides, excluding the 3' poly(A) tail, displaying the typical Alphacoronavirus-1 organization and the highest genetic relatedness to CCoV-II. However, two distinct features were observed in the CCoV-I genome: i) the presence of an additional ORF between the spike (S) protein gene and ORF3a; ii) the diversity of the S protein, which is more closely related to that of feline coronavirus type I and presents a furin cleavage site. The present study may contribute to a better understanding of the Alphacoronavirus-1 evolutionary pattern and may be paradigmatic of how coronaviruses evolve through gene losses, acquisition and exchanges among different members. Copyright © 2015. Published by Elsevier B.V.
    Virus Research 07/2015; DOI:10.1016/j.virusres.2015.07.018 · 2.83 Impact Factor
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    ABSTRACT: In order to evaluate sheep as experimental model to test the efficacy of HoBi-like pestivirus vaccines for cattle, 10 sheep at different stages of pregnancy (30 or 50 days) were experimentally infected with the Italian prototype isolate Italy-1/10-1. Irrespective of the stage of pregnancy, virus inoculation resulted in reproductive failures, consisting of abortion, stillbirths or birth of weak or persistently infected (PI) lambs. Aborted fetuses, stillborn and dead lambs displayed extensive histopathological changes, consisting of hemorrhages, congestion and mononuclear infiltration in major organs. Pestiviral antigens were detected by immunohistochemistry in most tissues with remarkable signals in lungs and kidneys. PI lambs were constantly viremic, shed the virus through the nasal secretions and feces and, in all cases but one, did not have detectable HoBi-like pestivirus antibodies before the assumption of colostrum. The single seropositive infected lamb showed low-titer viremia and viral shedding that ceased only several weeks after the 3-month observation period. The study proves that sheep are susceptible to the reproduction failures caused by HoBi-like pestivirus infection and can serve as a suitable model for the evaluation of the fetal protection induced by homologous experimental vaccines. Copyright © 2015 Elsevier B.V. All rights reserved.
    Veterinary Microbiology 05/2015; 178(3-4). DOI:10.1016/j.vetmic.2015.05.011 · 2.73 Impact Factor
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    ABSTRACT: Pantropic canine coronavirus (CCoV) was first detected in young dogs in Italy in 2005, but the complete genome sequence of this virus had not yet been determined. Here, we report the full-length genome sequence of the prototype strain CB/05, which showed that this virus is genetically similar to CCoV-IIa viruses. FOOTNOTES Address correspondence to Nicola Decaro, nicola.decaro{at} Citation Decaro N, Mari V, Dowgier G, Elia G, Lanave G, Colaianni ML, Buonavoglia C. 2015. Full-genome sequence of pantropic canine coronavirus. Genome Announc 3(3):e00401-15. doi:10.1128/genomeA.00401-15. Received 20 March 2015. Accepted 3 April 2015. Published 7 May 2015.
    Genome Announcements 05/2015; 3(3). DOI:10.1128/genomeA.00401-15
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    ABSTRACT: Clinical, virological and serological analyses of a natural case of caprine herpesvirus 1 (CpHV-1) infection in a buck, are reported. Three days after mating with a CpHV-1-infected female goat, the buck developed lesions referable to genital CpHV-1 infection. In particular, the animal suffered from typical painful ulcerative-crusted lesions associated with hyperemia, edema of the prepuce and healed completely within 15 days post-infection (p.i.). Infectious CpHV-1 was isolated from preputial swabs for 9 days p.i. while the virus was detected by real-time-PCR for 24 days p.i.. Neutralizing antibodies were detected 7 days p.i. reaching maximal titers by day 14 p.i.
    Small Ruminant Research 05/2015; 128. DOI:10.1016/j.smallrumres.2015.04.015 · 1.10 Impact Factor
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    ABSTRACT: The latex of Ficus carica Linn. (Moraceae) has been shown to interfere with the replication of caprine herpesvirus (CpHV)-1 in vitro. The present study was undertaken to determine the efficacy of vaginal administration of fig latex in goats experimentally infected with CpHV-1. The fig latex reduced the clinical signs of the herpetic disease although it slightly influenced the titres of CpHV-1 shed. Thus, the fig latex maintained a partial efficacy in vivo.
    Natural product research 04/2015; DOI:10.1080/14786419.2015.1028061 · 1.23 Impact Factor
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    ABSTRACT: Caprine herpesvirus 1 (CpHV-1) infection in goats induces genital vesicular-ulcerative lesions that strictly resemble the lesions induced by herpesvirus 2 in the human host. The immunosuppressive drug Mizoribine (MIZ) was found to increase the antiviral activity of Acyclovir (ACV) against herpesvirus infections, raising interesting perspectives on new combined therapeutic strategies. In this study the anti-CpHV-1 activity in vitro of ACV alone or in combination with MIZ was characterized. When applied alone at non-toxic concentrations, ACV had a slight effect on CpHV-1 replication while in combination with MIZ a dose-dependent inhibition of the virus yield was observed with an IC50 of ACV of 28.5 µM. These findings suggest that combined therapy of ACV and MIZ is potentially exploitable in the treatment of genital infection by herpesviruses.
    Research in Veterinary Science 01/2015; 99. DOI:10.1016/j.rvsc.2015.01.009 · 1.51 Impact Factor
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    ABSTRACT: Voltage-gated sodium channels are known to play a pivotal role in perception and transmission of pain sensations. Gain-of-function mutations in the genes encoding the peripheral neuronal sodium channels, hNav1.7-1.9, cause human painful diseases. Thus while treatment of chronic pain remains an unmet clinical need, sodium channel blockers are considered as promising druggable targets. In a previous study, we evaluated the analgesic activity of sumatriptan, an agonist of serotonin 5HT1B/D receptors, and some new chiral bioisosteres, using the hot plate test in the mouse. Interestingly, we observed that the analgesic effectiveness was not necessarily correlated to serotonin agonism. In this study, we evaluated whether sumatriptan and its congeners may inhibit heterologously expressed hNav1.7 sodium channels using the patch-clamp method. We show that sumatriptan blocks hNav1.7 channels only at very high, supratherapeutic concentrations. In contrast, its three analogs, namely 20b, (R)-31b, and (S)-22b, exert a dose and use-dependent sodium channel block. At 0.1 and 10 Hz stimulation frequencies, the most potent compound, (S)-22b, was 4.4 and 1.7 fold more potent than the well-known sodium channel blocker mexiletine. The compound induces a negative shift of voltage dependence of fast inactivation, suggesting higher affinity to the inactivated channel. Accordingly, we show that (S)-22b likely binds the conserved local anesthetic receptor within voltage-gated sodium channels. Combining these results with the previous ones, we hypothesize that use-dependent sodium channel blockade contributes to the analgesic activity of (R)-31b and (S)-22b. These later compounds represent promising lead compounds for the development of efficient analgesics, the mechanism of action of which may include a dual action on sodium channels and 5HT1D receptors.
    Frontiers in Pharmacology 01/2015; 6:155. DOI:10.3389/fphar.2015.00155 · 3.80 Impact Factor
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    ABSTRACT: Canine distemper virus (CDV) is the cause of a severe and highly contagious disease in dogs. The unpredictable and variable course of CDV-related disease may hamper correct diagnosis of infection and makes it crucial the collection of samples suitable for laboratory confirmation. In the present study we were able to follow the disease in two dogs infected naturally, collecting different biological matrices during the entire period of infection. By real time RT-PCR, viral RNA was detected and quantified, suggesting that urine and rectal swabs would be useful for ante-mortem diagnosis of distemper in dogs, regardless of the clinical stage and form of the illness. Copyright © 2014. Published by Elsevier B.V.
    Journal of Virological Methods 12/2014; 213. DOI:10.1016/j.jviromet.2014.12.004 · 1.88 Impact Factor
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    ABSTRACT: Noroviruses (NoVs) of genogroup IV (GIV) (Alphatron-like) cause infections in humans and in carnivorous animals such as dogs and cats. We screened an age-stratified collection of serum samples from 535 humans in Italy, using virus-like particles of genotypes GIV.1, circulating in humans, and GIV.2, identified in animals, in ELISA, in order to investigate the prevalence of GIV NoV-specific IgG antibodies. Antibodies specific for both genotypes were detected, ranging from a prevalence of 6.6% to 44.8% for GIV.1 and from 6.8% to 15.1% for GIV.2 among different age groups. These data are consistent with a higher prevalence of GIV.1 strains in the human population. Analysis of antibodies against GIV.2 suggests zoonotic transmission of animal NoVs, likely attributable to interaction between humans and domestic pets. This finding, and recent documentation of human transmission of NoVs to dogs, indicate the possibility of an evolutionary relationship between human and animal NoVs.
    Emerging infectious diseases 11/2014; 20(11):1828-32. DOI:10.3201/eid2011.131601 · 7.33 Impact Factor
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    ABSTRACT: Parvoviruses represent the most important infectious agents that are responsible for severe to fatal disease in carnivores. This study reports the results of a 10-year molecular survey conducted on carnivores in Bulgaria (n = 344), including 262 dogs and 19 cats with gastroenteritis, and 57 hunted wild carnivores. Real-time polymerase chain reaction (qPCR), followed by virus characterization by minor groove binder (MGB) probe assays, detected 216 parvovirus positive dogs with a predominance of canine parvovirus type 2a (CPV-2a, 79.17%) over CPV-2b (18.52%) and CPV-2c (2.31%). Rottweilers and German shepherds were the most frequent breeds among CPV-positive pedigree dogs (n = 96). Eighteen cats were found to shed parvoviruses in their faeces, with most strains being characterized as FPLV (n = 17), although a single specimen tested positive for CPV-2a. Only two wild carnivores were parvovirus positive, a wolf (Canis lupus) and a red fox (Vulpes vulpes), both being infected by CPV-2a strains.
    Transboundary and Emerging Diseases 11/2014; DOI:10.1111/tbed.12285 · 3.12 Impact Factor
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    ABSTRACT: Dog circovirus (DogCV) was identified in an outbreak of enteritis in pups in Italy. The disease was observed in 6 young dachshunds pups of a litter from a breeding kennel and caused the death of 2 dogs. Upon full-genome analysis, the virus detected in one of the dead pups (strain Bari/411-13) was closely related to DogCVs that have been recently isolated in the USA. The present study, if corroborated by further reports, could represent a useful contribution to the knowledge of the pathogenic potential of DogCV and its association with enteritis in dogs.
    PLoS ONE 08/2014; 9(8):e105909. DOI:10.1371/journal.pone.0105909 · 3.23 Impact Factor
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    ABSTRACT: During the first weeks of life puppies remain protected against canine parvovirus type 2 (CPV2) infection thanks to maternally derived antibodies (MDA) absorbed with colostrum after birth. The objective of the present study was to present the variability in CPV2-specific passive immune transfer and its consequences in puppies naturally exposed to the parvovirus. Seventy-nine puppies from one breeding kennel were included in the study at birth and fol-lowed until 56 d of age. Once per week the MDA titre for CPV2 specific antibodies was determined in blood. Viral excretion was also evaluated on a rectal swab by CPV2 PCR assay and puppies were weighed to determine growth rate. At 2 d of age, thirty-four out of seventy-nine puppies (43 %) had MDA ≤1:160 (designed group A) and forty-five puppies (57 %) had greater MDA titres (designed group B). The level of absorbed maternal antibodies was shown to be associated with breed size and growth rate during the first 48 h of life. The MDA level declined with age in all cases; however, the proportion of puppies with the antibody level considered as protective against CPV2 infection was significantly higher in group B compared with A from day 2 until 42. Among all puppies surviving until 56 d of age, sixty-seven out of seventy (95·7 %) underwent CPV2 infection. However, puppies from group A excreted CPV2 significantly earlier than puppies from group B. The present study demonstrates the link between passive immune transfer, in terms of level of specific MDA absorbed, and length of the protection period against parvovirus infection in weaning puppies.
    07/2014; 3(1). DOI:10.1017/jns.2014.57
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    ABSTRACT: A calf persistently infected (PI) by Hobi-like pestivirus displayed severe clinical signs and subsequent death. Gross lesions and histopatological changes were suggestive of haemorrhagic and necrotic inflammation involving several tissues. A Hobi-like pestivirus pair was isolated from the dead calf, with the cytopathogenic (cp) and noncytopathogenic (ncp) strains being strictly related to each other and to Italian prototype isolates at the genetic level. Two biotype-specific real-time RT-PCR assays dated back the emergence of the cp virus to one month before the death. This reached the highest RNA titres in the lymphoid and nervous tissues, whereas only traces of cp viral RNA were found in the blood. In contrast, the ncp virus was present at high loads in all tissues and biological fluids. The present report provides new insights into the comprehension of the pathogenesis and molecular mechanisms of this emerging group of pestiviruses.
    Journal of Clinical Microbiology 06/2014; 52(8). DOI:10.1128/JCM.00986-14 · 4.23 Impact Factor
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    ABSTRACT: The latex of Ficus carica Linn. (Moraceae) has been shown to possess antiviral properties against some human viruses. To determine the ability of F. carica latex (F-latex) to interfere with the infection of caprine herpesvirus-1 (CpHV-1) in vitro, F-latex was resuspended in culture media containing 1% ethanol and was tested for potential antiviral effects against CpHV-1. Titration of CpHV-1 in the presence or in the absence of F-latex was performed on monolayers of Madin Darby Bovine Kidney (MDBK) cells. Simultaneous addition of F-latex and CpHV-1 to monolayers of MDBK cells resulted in a significant reduction of CpHV-1 titres 3 days post-infection and this effect was comparable to that induced by acyclovir. The study suggests that the F-latex is able to interfere with the replication of CpHV-1 in vitro on MDBK cells and future studies will determine the mechanisms responsible for the observed antiviral activity.
    Natural Product Research 05/2014; 28(22):1-5. DOI:10.1080/14786419.2014.918120 · 1.23 Impact Factor
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    ABSTRACT: Canine parvovirus (CPV) modified live virus vaccines are able to infect vaccinated dogs replicating in the bloodstream and enteric mucosa. However, the exact duration and extent of CPV vaccine-induced viremia and fecal shedding are not known. With the aim to fill this gap, 26 dogs were administered two commercial vaccines containing a CPV-2 or CPV-2b strain and monitored for 28 days after vaccination. By using real-time PCR, vaccine-induced viremia and shedding were found to be long lasting for both vaccinal strains. Vaccinal CPV-2b shedding was detected for a shorter period than CPV-2 (12 against 19 mean days) but with greater viral loads, whereas viremia occurred for a longer period (22 against 19 mean days) and with higher titers for CPV-2b. Seroconversion appeared as early as 7 and 14 days post-vaccination for CPV-2b and CPV-2 vaccines, respectively. With no vaccine there was any diagnostic interference using in-clinic or hemagglutination test, since positive results were obtained only by fecal real-time PCR testing. The present study adds new insights into the CPV vaccine persistence in the organism and possible interference with diagnostic tests.
    Vaccine 04/2014; 32(30). DOI:10.1016/j.vaccine.2014.04.050 · 3.49 Impact Factor

Publication Stats

6k Citations
718.57 Total Impact Points


  • 1993–2015
    • Università degli Studi di Bari Aldo Moro
      • Dipartimento di Medicina Veterinaria
      Bari, Apulia, Italy
  • 2010
    • Agricultural University of Athens
      • Faculty of Animal Science and Aquaculture
      Athens, Attiki, Greece
  • 2009
    • Hungarian Academy of Sciences
      Budapeŝto, Budapest, Hungary
  • 2006
    • Government of the People's Republic of China
      Peping, Beijing, China
  • 1984–2006
    • Istituto Superiore di Sanità
      • • Department of Infectious, Parasitic and Immune-mediated Diseases
      • • Department of Food Safety and Veterinary Public Health
      Roma, Latium, Italy
  • 2001
    • Cornell University
      • College of Veterinary Medicine
      Ithaca, New York, United States
  • 1998
    • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna
      Brescia, Lombardy, Italy
  • 1997
    • University of Naples Federico II
      • Department of Veterinary Medicine
      Napoli, Campania, Italy
  • 1992
    • Università degli Studi Europea di Roma
      Roma, Latium, Italy
  • 1987
    • University of Milan
      Milano, Lombardy, Italy