Chun-Shi Liang

The University of Tokyo, Edo, Tōkyō, Japan

Are you Chun-Shi Liang?

Claim your profile

Publications (6)13.83 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: We determined coding sequences for three types of grass carp myosin subfragment-1 (S1) heavy chain by extending 5'-regions of the three known genes encoding light meromyosin isoforms (10 degrees C, intermediate and 30 degrees C types). The primary structures of these three S1 heavy chain isoforms showed 81.4%, 81.2%, and 97.8% identities between the 10 degrees C and intermediate types, between the 10 degrees C and 30 degrees C types, and between the intermediate and 30 degrees C types, respectively. Isoform-specific differences were clearly observed between the 10 degrees C type and the other two types in 97 amino acid residues. Furthermore, among these amino acid mutations, 51 mutations occurred at the conserved residue sites of S1 heavy chain from fish and homoiotherm. Additionally, the 10 degrees C type showed striking differences compared with the other two types in the two surface loops, loop 1 located near the ATP-binding pocket and loop 2, which is one of the actin-binding sites, suggesting that such structural differences possibly affect their motor functions. Interestingly, this 10 degrees C-type myosin heavy chain isolated from adult grass carp skeletal muscle was surprisingly similar to the embryonic fast-type myosin heavy chain from juvenile silver carp in the structure of S1 heavy chain, indicating that it may also function as embryonic fast-type myosin heavy chain in juvenile stage.
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 07/2009; 154(2):248-54. DOI:10.1016/j.cbpb.2009.06.013 · 1.61 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The three types of cDNA clones, previously defined as the 10 degrees C, intermediate and 30 degrees C-types [Tao, Y., Kobayashi, M., Liang, C.S., Okamoto, T., Watabe, S., 2004. Temperature-dependent expression patterns of grass carp fast skeletal myosin heavy chain genes. Comp. Biochem. Physiol. B 139, 649-656], were determined for their 5'-regions which encoded at least the C-terminal half of myosin rod, light meromyosin (LMM), in fast skeletal muscles of grass carp Ctenopharyngodon idella. The deduced amino acid sequence identity was 91.1% between the 10 degrees C and 30 degrees C-types and 91.4% between the 10 degrees C and intermediate-types, whereas a high sequence identity of 97.8% was found between the intermediate and 30 degrees C-types. These three grass carp LMMs all had a characteristic seven-residue (heptad) repeat (a, b, c, d, e, f, g)(n), where positions a and d were normally occupied by hydrophobic residues, and positions b, c and f by charged residues. However, the ratios of hydrophobic residues to the total were higher for the intermediate- and 30 degrees C- than 10 degrees C-type LMM, suggesting that the former both types may form more stable coiled-coils of alpha-helices than the latter type. These differences in the primary structures of LMM isoforms might be partially implicated in differences in the thermostabilities and gel-forming profiles of myosins from grass carp in different seasons reported previously [Tao, Y., Kobayashi, M., Fukushima, H., Watabe, S., 2005. Changes in enzymatic and structural properties of grass carp fast skeletal myosin induced by the laboratory-conditioned thermal acclimation and seasonal acclimatization. Fish. Sci. 71, 195-204; Tao, Y., Kobayashi, M., Fukushima, H., Watabe, S., 2007. Changes in rheological properties of grass carp fast skeletal myosin induced by seasonal acclimatization. Fish. Sci. 73, 189-196].
    Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology 03/2008; 149(2):378-87. DOI:10.1016/j.cbpb.2007.10.012 · 1.90 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: We characterized the promoter activity of fast skeletal myosin heavy chain genes (MYHs) from medaka Oryzias latipes. The 5'-flanking region of approximately 6 kb in medaka MYHs, mMYH10 and mMYH30, predominantly expressed in medaka acclimated to 10 degrees C and 30 degrees C, respectively, contained various cis-elements that are supposed to bind to transcriptional regulatory factors such as MyoD and myocyte enhancer factor 2 (MEF2) family members and nuclear factor of activated T cells. To localize functional regions responsible for the mMYH expression in a temperature-dependent manner, a series of deletion and site mutation constructs prepared from the 5'-flanking regions were fused to the luciferase gene in a commercially available plasmid and directly injected into the dorsal fast muscle of medaka acclimated to 10 degrees C and 30 degrees C. The truncation of MEF2 binding site located at -966 to -957 in the 5'-flanking region of mMYH10 resulted in distinct gene expression at 10 degrees C. The activation effect by the removal of this binding site was further confirmed by the mutation construct. One of the E box sites, to which MyoD family members are supposed to bind, was located at -613 to -607 of mMYH10, and found to be responsible for the transcriptional activity. In contrast, the MEF2 binding site located at -960 to -951 of mMYH30 was involved in the activation at 30 degrees C. Thus, these transient transfection assays demonstrated that the MEF2 binding site is crucial for a temperature-dependent expression of mMYHs.
    Gene 02/2008; 407(1-2):42-53. DOI:10.1016/j.gene.2007.09.016 · 2.08 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To disclose mechanisms involved in temperature acclimation of fish muscle, we subjected eurythermal fish of medaka Oryzias latipes to cloning of myosin heavy chain genes (MYHs). We cloned cDNAs encoding fast skeletal muscle myosin heavy chain (MYH) isoforms from cDNA libraries of medaka acclimated to 10 and 30 degrees C and observed that different MYH cDNA clones are expressed in the two temperature-acclimated fish. Subsequently, we isolated several overlapping MYH contigs by shotgun cloning strategy from a medaka genomic library. Contig assembly of the complete medaka MYH (mMYH) locus of 219 kbp revealed a cluster of tandemly arrayed 11 mMYHs, in which eight genes are actually transcribed, with the remaining three being pseudogenes. Expression analysis of the transcribed genes revealed that two genes were each highly expressed in medaka acclimated to 10 and 30 degrees C, whereas comparatively lower expression levels of the three genes were exclusively observed in medaka acclimated to 30 degrees C. cDNAs of the remaining genes were too underrepresented in the libraries to determine the expression levels, and the transcripts could only be obtained by reverse transcription-polymerase chain reaction. Deduced amino acid sequences in the loop 1 and loop 2 regions of mMYHs were highly variable, suggesting that these isoforms were functionally different. The present findings consolidate our knowledge on teleost MYH multigene family and would provide further insight into the mechanisms by which expressions of individual MYH molecules are fine-tuned with environmental temperature fluctuations with further functional analysis of the genes concerned.
    Physiological Genomics 05/2007; 29(2):201-14. DOI:10.1152/physiolgenomics.00078.2006 · 2.81 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Three adenine nucleotide translocator (ANT) genes were identified through in silico data mining of the Fugu genome database along with isolation of their corresponding cDNAs in vivo from the pufferfish (Takifugu rubripes). As a result of phylogenetic analysis, the ANT gene on scaffold_254 corresponded to mammalian ANT1, whereas both of those on scaffold_6 and scaffold_598 to mammalian ANT3. The ANT gene encoded by scaffold_6 was expressed ubiquitously in various tissues, whereas the ANT genes encoded by scaffold_254 and scaffold_598 were predominantly expressed in skeletal muscle and heart, respectively.
    Mitochondrion 07/2005; 5(3):162-72. DOI:10.1016/j.mito.2005.01.003 · 3.52 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Three types of myosin heavy chain cDNA clone named 10 degrees C, intermediate and 30 degrees C types were isolated from fast skeletal muscles of thermally acclimated grass carp Ctenopharyngodon idellus. Three clones encompassing parts of 3'-translated and entire 3'-untranslated regions showed high heterogeneities in their nucleotide sequences in the 3'-untranslated region. The comparison in the deduced amino acid sequence of the 10 degrees C-type clone with those of the intermediate- and 30 degrees C-type clones showed 88% and 89% identities, respectively. By contrast, the deduced amino acid sequence of the intermediate-type clone shared much higher identity of 97% with its 30 degrees C-type counterpart. Northern blot analysis demonstrated that the 10 degrees C- and 30 degrees C-type clones were predominantly expressed in grass carp acclimated to 10 and 30 degrees C, respectively. The intermediate type was expressed both in grass carp acclimated to 20 and 30 degrees C. Furthermore, expression patterns of the three myosin heavy chain genes were altered in accompaniment with seasonal temperature fluctuation. In autumn and winter grass carp expressed the 10 degrees C-type gene almost exclusively, whereas it was completely replaced by the intermediate- and 30 degrees C-type genes in spring and summer. These results suggest that tetraploid grass carp also undergo an adaptation to fluctuating environmental temperatures by selectively expressing fast skeletal myosin heavy chain isoforms as do diploid common carp previously reported.
    Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology 01/2005; 139(4):649-56. DOI:10.1016/j.cbpc.2004.08.007 · 1.90 Impact Factor