Christiane Bébéar

Université Victor Segalen Bordeaux 2, Burdeos, Aquitaine, France

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Publications (23)35.08 Total impact

  • Journal of forensic and legal medicine 05/2011; 18(4):187.
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    ABSTRACT: The objective of this study was to determine the effect of antibiotics on Chlamydia trachomatis viability by using a quantitative real-time PCR assay that measured DNA replication and mRNA transcription of the structural omp1 and omp2 genes, 16S rRNA and the groEL1 gene with and without antibiotics. Ofloxacin, moxifloxacin, azithromycin and doxycycline were tested against the serovar D and L2 reference strains and a derivative mutant resistant to fluoroquinolones, L2-OFXR, obtained by in vitro selection. Using DNA quantification, the antibiotic MIC was calculated when the number of DNA copies was equal to that of the chlamydial inoculum at time zero. This method allowed the easy determination of MICs by DNA quantification of the four selected genes and gave similar results to those obtained by immunofluorescence staining without biased interpretation. By using cDNA quantification, the lowest antibiotic concentration for which no RNA was transcribed corresponded to the minimum bactericidal concentration. C. trachomatis still transcribed the16S rRNA and groEL1 genes, even at concentrations well above the MIC, showing a bacteriostatic effect for all antibiotics tested. This method allows the study of antibiotic activity on growth and viability of C. trachomatis by DNA and RNA quantification at the same time without additional cell-culture passaging.
    Journal of Medical Microbiology 12/2010; 60(Pt 4):508-14. · 2.30 Impact Factor
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    ABSTRACT: A 7-year old girl suspected of having been sexually abused owing to the presence of anal condyloma was found to be infected by Chlamydia trachomatis. Microbiological analysis and anamnesis were consistent with the infection having been acquired at birth. This case confirms that untreated infection acquired at birth can persist for months or years and highlights the value of examining those involved in the suspicion of sexual abuse of the child.
    Journal of forensic and legal medicine 02/2010; 17(2):96-8.
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    ABSTRACT: We read with extreme interest the article by Moncada et al. (2) about the use of self-collected glans and rectal swabs for detection of Chlamydia trachomatis (CT) in symptomatic and asymptomatic men who have sex with men (MSM). ...
    Journal of clinical microbiology 07/2009; 47(8):2686. · 4.16 Impact Factor
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    ABSTRACT: We developed a real-time High Resolution Melting PCR to identify the new Swedish variant of Chlamydia trachomatis ncCT. Of 1191 urogenital specimens C. trachomatis-positive by an omp1 real-time PCR, collected in France in 2007-2008, 1128 gave an interpretable profile corresponding to the wild-type strain; no nvCT was found. This test can be used on selected C. trachomatis-positive samples to monitor the nvCT spread.
    Journal of microbiological methods 06/2009; 78(1):101-3. · 2.43 Impact Factor
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    ABSTRACT: Five severe cases of psittacosis in individuals associated with duck farms were notified in France between January and March 2006. Diagnostic examination included serology and/or molecular detection by PCR from respiratory samples. As a consequence, we investigated all duck flocks (n=11) that were housed in the three farms where human infections occurred. While serology by complement fixation test was negative for all samples, cloacal and/or tracheal chlamydial excretion was detected by PCR in all three units. Notably, one duck flock was tested strongly positive in 2 of the 3 affected farms, and Chlamydophila (C.) psittaci strains were isolated from cloacal and/or tracheal swab samples from both farms. Human samples and duck isolates exhibited the same PCR-RFLP restriction pattern, which appeared to be an intermediate between genotypes A and B. Analysis of ompA gene sequences and comparison to those of the type strains showed that the isolates could not be strictly assigned to any of the generally accepted genotypes of C. psittaci. Further analysis by MLVA of the PCR-positive human samples revealed two distinct patterns, which were related to previously isolated C. psittaci duck strains.
    Veterinary Microbiology 10/2008; 135(1-2):82-9. · 3.13 Impact Factor
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    ABSTRACT: Mycoplasmas and chlamydiae, bacteria involved in respiratory tract and urogenital infections, differ by their growth requirements, acellular specific media for mycoplasmas, cell culture methods for chlamydiae. Tetracyclines, macrolides, lincosamides, streptogramins and ketolides (MLSKS) and fluoroquinolones, are active against both microorganisms. However intrinsic resistance to certain MLSKs are observed in M. hominis and Ureaplasma spp., two genital mycoplasmas. Acquired resistance has been reported in clinical isolates of M. hominis and Ureaplasma spp., and linked to therapeutic failure. It concerns mainly tetracyclines and is due to the presence of the tet(M) gene. Acquired resistance to macrolides and fluoroquinolones, very rarely observed in these two species, mainly in immunosuppressed patients, is linked to mutations in the antibiotic target. For M. pneumoniae, only resistance to macrolides has been reported in a small number of clinical isolates, while mutants resistant to fluoroquinolones and tetracyclines were obtained in vitro. Antibiotic resistance has been reported very rarely for M. genitalium, with some therapeutic failures related to resistant strains described after treatment by macrolides or fluoroquinolones. In these two last species, molecular diagnosis of acquired resistance has been related to target alterations.Acquired resistance is more controversial in chlamydiae. Clinical treatment failures have been attributed to C. trachomatis strains exhibiting heterotypic multidrug resistance, concerning only a small number of organisms. Induction of resistance has been observed in vitro but homotypic stable resistance has been demonstrated only in swine isolates. Heterotypic stable resistance could correspond more to the induction of a persistent phenotype refractory to multiple antibiotics and its responsibility in therapeutic failure is still not clear.
    Revue Francophone des Laboratoires 01/2007; 2007(391):77-85.
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    ABSTRACT: For screening the C. trachomatis infection in asymptomatic women, we compared the performances of self-collected vaginal swab with those of cervical swab by using Cobas TagMan® (Roche Diagnostics). Cob's TagMan® utilizes real time PCR technology on the dual labeled fluorescent probe and allows an estimation of bacterial load. For each patient, two cervical specimens were taken and discharged in two transport medium, 2SP and M4RT and the patient self collected a dry vaginal specimen. The C. trachomatis detection in cervical specimens was made by using Cobas Amplicor® and Cobas Tagmano® and in vaginal specimens by Cobas TagMan®. Sensitivity and specificity of both analysers and both transport media were quite identical. The self-collected vaginal swab was better to detect C. trachomatis infection than cervical swab but the chlamydial load was higher in cervix compared with vagina. Vaginal swab is an appropriate specimen for diagnosing genital chlamydial infection and Cobas TagMan® could replace Cobas Amplicor®.
    Revue Francophone des Laboratoires 01/2007; 2007(391):87-90.
  • Christiane Bébéar, Cécile M. Bébéar
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    ABSTRACT: Five mycoplasma species, Mycoplasma pneumoniae, M. hominis, M. genitalium, Ureaplasma urealyticum and U. parvum, here designed as Ureaplasma spp., are responsible for human infections.M. pneumoniae is the only mycoplasma clearly pathogen for the respiratory tract. Implicated most often in trachéobronchtes, it is the second agent responsible for bacterial community-acquired pneumonia and is probably involved in asthma exacerbation. M. pneumoniae infections occur endemically with epidemic peaks every 4 – 7 years, mostly in children above 5 years. The biological diagnosis of these infections is made only in severe infections, mainly by serology because of the fastidious character of the microorganism. However, M. pneumoniae can be easily detected by molecular amplification techniques.The other mycoplasma species are mainly responsible for infections of the urogenital tract. M. genitalium is the second agent of non gonococcal urethritis after Chlamydia trachomatis. Ureaplasma spp. is also involved in chronic urethritis but can be present, like M. hominis but more often, as a commensal of the lower genital tract. All species are responsible for infections of the genital tract of women. Ureaplasma spp. and M. hominis, other species rarely, are involved in infections in immunocompromised patients. The only method adapted to the detection of M. genitalium, a very fastidious mycoplasma, is amplification technique. The other species can relatively easily be detected by culture but the main problem is to interpret the results because of their possible presence as commensals.
    Revue Francophone des Laboratoires 01/2007; 2007(391):63-69.
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    ABSTRACT: Chlamydia trachomatis is among the most common of the human pathogens and pose a public health problem given their frequency and their complications as salpingitis, infertility and ectopic pregnancy. Some recent prevalence studies showed that health authorities should consider planning prevention by systematic screening by using self collected specimens, first void urine for men and vaginal swab for women in familiy planning clinic and abortion clinic. Only nucleic acid amplification test can detect C. trachomatis in these samples. The outbreak of rectal lymphogranuloma venereum (LGV) requires a microbiological and clinical surveillance of men presenting a rectitis. A confirmed case is a case with a positive PCR test for C. trachomatis in rectal specimen and an L genotype as determined by RFLP A high serological titer could be evocative. C. pneumoniae is responsible for respiratory infection, under diagnosed given the mild character of this infection and the lack of accurate tests. The avian strains of C. psittaci can infect humans. Most infections are through inhalation of infectious aerosol which occurred when infected animal and/or contaminated material are handled. Contaminated faeces and feathers may play an essential role for infection transmission from flocks to humans. The chlamydiosis' symptoms are variable, ranging from no clinical signs at all to severe systemic disease. The diagnosis is supported by clinical, epidemiological and biological features.
    Revue Francophone des Laboratoires 01/2007; 2007(391):71-76.
  • Christiane Bébéar, Sabine Pereyre, Cécile M. Bébéar
    12/2006: pages 305 - 315; , ISBN: 9780470017968
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    ABSTRACT: The objectives were to estimate the prevalence of Chlamydia trachomatis infection in subfertile couples and to study the relationship between markers of C. trachomatis infection and male infertility as well as pregnancy rates after in vitro fertilization (IVF). All consecutive couples consulting for infertility and IVF in Pellegrin Hospital were screened for C. trachomatis by direct (PCR test) and serological methods. Two hundred and seventy-seven couples were included in the study (mean age in years: 35 for men, 32 for women; mean duration of infertility: 4 years). The most frequent indication for IVF was tubal factor in 33%, endometriosis in 6%, dysovarian function in 12%, male infertility in 36% and others in 13%. C. trachomatis PCR was positive in 1.2% of men, 95% confidence interval (CI95%): (0.2%; 3.3%) and in 2.7% of women, CI95%: (1.1%; 5.5%). When combining all chlamydial markers, 17.3% of men, CI95%: (12.7%; 22.8%) and 20.4% of women, CI95%: (15.6%; 25.9%) had at least one positive marker. The presence of positive markers was not associated with altered semen characteristics. Couples with positive markers had a pregnancy rate of 23.1% (12 out of 52) compared with 20.2% (24 out of 119) among those with negative markers. In this population, the presence of past or current C. trachomatis infection was associated with neither semen characteristics nor outcome of IVF in subfertile couples.
    European Journal of Obstetrics & Gynecology and Reproductive Biology 12/2006; 129(1):46-53. · 1.84 Impact Factor
  • Journal of Medical Microbiology 05/2006; 55(Pt 4):471-3. · 2.30 Impact Factor
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    ABSTRACT: This report describes the case of a patient with a 14-month course of severe oligoarthritis associated with acne. Pure cultures of Propionibacterium acnes were isolated from synovial tissue and synovial fluid specimens collected from the same joint after a 4-month interval. After 2 months of treatment with roxithromycin 300 mg/day, rifampicin 1,200 mg/day, and a nonsteroidal antiinflammatory drug (NSAID), followed by 4 months of treatment with azithromycin 1 gm/week and and NSAID, the synovitis persisted. Cultures of skin lesions and synovial fluid at this time were negative. Although P acnes has previously been isolated from bone specimens obtained from patients with osteitis associated with acne, this is the first report of the isolation of this microorganism from the synovial tissue of a patient with arthritis associated with acne. Our findings raise the question of the role of P acnes in the pathogenesis of arthritis associated with acne.
    Arthritis & Rheumatology 05/2004; 41(10):1889 - 1893. · 7.48 Impact Factor
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    ABSTRACT: Fourier transform infrared (FT-IR) spectroscopy is a convenient physico-chemical technique to investigate various cell materials. Bacteria of class Mollicutes, identified by conventional methods, as Mycoplasma, Acholeplasma and Ureaplasma genera were characterized using this method. A data set of 74 independent experiments corresponding to fourteen reference strains of Mollicutes was examined by FT-IR spectroscopy to attempt a spectral characterization based on the biomolecular structures. In addition to the separation of Mollicutes within the lipidic region into five main clusters corresponding to the three phylogenetic groups tested, FT-IR spectroscopy allowed a fine discrimination between strains belonging to the same species by using selective spectral windows, particularly in the 1200-900 cm(-1) saccharide range. The results obtained by FT-IR were in good agreement with both taxonomic and phylogenetic classifications of tested strains. Thus, this technique appears to be a useful tool and an accurate mean for a rapid characterization of Mollicutes observed in humans.
    Journal of Microbiological Methods 02/2004; 56(1):73-82. · 2.16 Impact Factor
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    ABSTRACT: Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n = 14; 40 %), followed by F (n = 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories.
    Journal of Medical Microbiology 02/2004; 53(Pt 1):47-50. · 2.30 Impact Factor
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    ABSTRACT: The prevalence of Chlamydia trachomatis infection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women (P = 0.026) and a previous sexually transmitted infection in men (P = 0.023). The most frequent genotypes determined by sequencing the omp1 genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
    Journal of Clinical Microbiology 10/2003; 41(9):4404-7. · 4.07 Impact Factor
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    Cécile M. Bébéar, Christiane Bébéar
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    ABSTRACT: Standardization of susceptibility testing of mycoplasmas should be recommended and has to be conducted. Considering the profile of acquired resistance in human mycoplasmas, M. pneumoniae and M. genitalium are predictably susceptible to antibiotics. Thus, their susceptibility testing is not necessary except for the in vitro evaluation of new antimicrobials. In contrast, the susceptibility of urogenital mycoplasmas needs to be evaluated, mainly in view of antibiotic resistance documented for these species. Extragenital or respiratory infections caused by mycoplasmas occur often in immunocompromized patients, submitted frequently to an antibiotic selection pressure. So, in these patients the risk of multidrug-resistant mycoplasmas is higher, leading to the necessity of in vitro susceptibility testing of these microorganisms. The appearance now of acquired resistance to the three main antibiotic classes used in mycoplasmal infections prompts us to keep watch over the susceptibility of human mycoplasmas, especially in immunocompromized patients. Furthermore, it is recommended to test recent isolates of mycoplasmas when studying a new compound, to be sure to include in the study some potential new cases of acquired resistance.
    12/2001: pages 545-566;
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    ABSTRACT: To determine the range of ocular strains of Chlamydia trachomatis circulating in southern Morocco, where trachoma is endemic, and to compare the value of the molecular methods for genotyping C. trachomatis, ocular specimens were subjected to a direct Omp1 PCR-restriction fragment length polymorphism (RFLP)-based analysis and direct sequencing. PCR-RFLP analysis shows that the Ba genotype represents the most frequent one (63%), followed by genotype A (45%), whereas no B or C genotypes were identified among the 53 out of 108 specimens that were strongly positive in the Omp1 CT1-CT5 PCR. Our results further show that the notion of interfamily and intrafamily transmission is very likely. To confirm the genotype identity of C. trachomatis as determined by PCR-RFLP, 16 selected specimens were sequenced across variable sequence 1 (VS1) and 2 (VS2). No discrepancies were found between PCR-RFLP typing and the genotype identity confirmed by nucleotide sequencing of the PCR product. Our results clearly indicate that both molecular methods of typing chlamydiae (i.e., PCR-RFLP and sequencing) are important and have specific applications for clinical epidemiological purposes. This is the case for individuals infected with more than one clonal population of C. trachomatis. The unambiguous nucleotide sequencing therefore defines an important epidemiologic descriptor for the infected patient whether the source is from a clonal population of organisms or whether it represents a more dynamic process of strain dominance or genetic change. Furthermore, Omp1 genotyping affords the necessary approach to epidemiologic investigations in areas of the world endemic for trachoma, where only one or two serovars are known to predominate.
    Microbes and Infection 06/2001; · 2.92 Impact Factor
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    ABSTRACT: The French “Agence du médicament”, in charge of reacto-vigilance, asked to a group of experts, to compare the sensitivity of all C. trachomatis diagnostic tests commercially available in France. Five laboratories took part in this comparison work. Each kit was tested by two laboratories. Nine dilution's yielding 105, 104, 103, 5.102, 102, 1, 10−1 and 10−2 inclusion forming units (IFU)/mL of the C. trachomatis strain were tested. Each dilution was tested in triplicate in each laboratory. The threshold of sensitivity of a kit was considered to be the last dilution tested that gaves six positive results for six analyses performed. The best results were obtained with the four nucleic acid amplification assays. DFA are also, in the majority very sensitive. Results obtained with EIA test were very different from one test to another, but on the whole those tests lacked sensitivity.
    Revue Française des Laboratoires. 10/1998; 1998(306).

Publication Stats

112 Citations
35.08 Total Impact Points

Institutions

  • 2001–2009
    • Université Victor Segalen Bordeaux 2
      • Institut de Santé Publique d'Epidémiologie et de Développement (ISPED)
      Burdeos, Aquitaine, France
    • University of Bordeaux
      Burdeos, Aquitaine, France
  • 2004
    • Pasteur Center of Cameroon
      Jaúnde, Centre Region, Cameroon