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Y Dieng,
D Sow,
M Ndiaye,
E Guichet, B Faye,
R Tine,
A Lo,
K Sylla,
A Abiola,
T Dieng,
J L Ndiaye,
P Le Pape,
O Gaye
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ABSTRACT: The frequency of candidiasis has increased dramatically in recent years. Candida albicans is the most common species. However, other species which are pathogenic and resistant to usual antifungal agents beginning to emerge. These include Candida dubliniensis and Candida africana, which share morphological similarities with Candida albicans. Thus, it is of interest to correctly identify the fungal isolates.
To seek these new species among Candida strains isolated in Dakar.
Oropharyngeal and vaginal swabs were performed at Fann Universitary Hospital in Dakar. The strains were identified by the germ tube test, the chlamydospore production test and an auxanogram. Then identification by PCR targeting the hyphal wall protein 1(hwp1) gene, was performed for the discrimination between Candida albicans, Candida dubliniensis and Candida africana.
In total, 243 yeasts were isolated from samples including 231 in vaginal swab and 12 in oropharyngeal swab. Species identified by phenotypic methods are Candida albicans, which is the most frequent, Candida tropicalis, Candida glabrata, Candida dubliniensis, Candida kefyr and Candida lusitaniae. PCR performed on the 150 strains germ tube test positive identifies three Candida africana, 109 Candida albicans and no strain of Candida dubliniensis.
This study isolates Candida africana for the first time in Senegal. Further studies on a larger sample will better know the actual proportion of these three species among the isolated yeasts.
Journal de Mycologie Médicale/Journal of Medical Mycology 12/2012; 22(4):335-40. · 0.73 Impact Factor
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ABSTRACT: The impact of intermittent presumptive treatment (IPT) on the immunity of pregnant women in Senegal is still not very well known. We conducted a prospective study at the Roi-Baudouin maternity of Guediawaye in Senegal to assess IgG antibodies production against MSP1, GLURP and DBL5 in pregnant women under IPT. Blood samples were collected from the participating women at inclusion and delivery. Samples were analyzed after centrifugation for the detection of IgG antibodies in sera by Elisa. Informed consent was given by each study participant prior to their inclusion. A total of 101 eligible women aged from 18 to 44 were included in this study. Multigravidae women represented 70.3% of the study population, whereas primigravidae accounted for 29.7%. The IgG level decreased slightly from inclusion to delivery for the women with regard to anti-MSP1 (83.1at inclusion versus 79.5 at delivery, p = 0.52) as well as anti-GLURP-R2 (84.1 at inclusion versus 75.9 at delivery, p = 0.16). After adjustment for number of pregnancies, there was a significant decrease in the production of anti-VAR2CSA between inclusion and delivery (p < 0.05). By reducing the incidence of malaria during pregnancy, IPT reduced the acquisition of placental parasites antibodies suppressors which could delay the development of protective immunity against malaria. The application of IPT in pregnant women would thus be more appropriate in hypoendemic areas where malaria exposure is lower.
Bulletin de la Société de pathologie exotique 08/2011; 104(4):277-83.
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ABSTRACT: Bad breath is a disease affecting 25% of the world's population. Despite the widespread nature of this problem, people's knowledge about this condition is poor.
The purpose of this study was to determine the prevalence of halitosis in a cohort of 62 Senegalese subjects with dental prostheses.
Breath analysis was based on measurement of volatile sulfur compounds (VSC) using a halimeter. Halitosis was diagnosed ifVSC level was > or = 125 ppb.
Mean VSC level was 157.7 +/- 152.6 ppb. The prevalence of halitosis was of 35.4% overall, 72.7% in persons with fixed dentures and of 27.3% in persons with removable prostheses.
The relatively high prevalence of halitosis observed in this study indicates a need to enhance patient awareness of daily hygiene measures for dental prosthesis.
Médecine tropicale: revue du Corps de santé colonial 06/2011; 71(3):272-4.
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ABSTRACT: The purpose of this study was to update data on toxoplasmosis antibody prevalence based on antenatal surveillance tests in pregnant women in Dakar, Senegal. The study population consisted of 941 pregnant women referred for diagnosis of toxoplasmosis in the Laboratory of Parasitology and Mycology at Le Dantec University Hospital Center from 2002 to 2006. Two tests using the solid-phase immunoenzymatic method were performed on venous blood samples collected at 3 weeks of interval (S1 and S2). This double testing technique was designed to detect increases in IgM and IgG antibody levels in order to confirm diagnosis of toxoplasmosis by ruling out immune response, acquired immunity or nonspecific antibody fixation. Comparison of S1 and S2 results in the 941 patients indicated a prevalence of 7.7% and 0% respectively for IgM+IgG- cases, 23.3% and 24.3% respectively for IgM+IgG-cases, and 11.3% and 10.2% respectively for IgM+IgG+ cases. The overall prevalence of toxoplasmosis antibodies was 34.5%. These data showing a high prevalence of toxoplasmosis among pregnant women in Dakar underscore the need to improve serological screening and follow up.
Médecine tropicale: revue du Corps de santé colonial 02/2011; 71(1):101-2.
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ABSTRACT: The goal of this study was to evaluate primary school teachers' knowledge regarding emergency management of avulsed permanent incisors.
The study was conducted in fifty randomly chosen primary schools from Casablanca, Morocco. All teachers of the selected schools were included in the study. The data were collected by self administered questionnaires. The questions focused on the teachers' general characteristics, experience of avulsed teeth and the importance of emergency management. The data were analyzed using chi square test.
A total of 501 teachers, of which 23.6% were male and 75.4% were female, answered the questionnaire. The results showed that 44.5 % of the teachers had an experience of avulsed tooth at school, 82.82% of them knew the importance of emergency management and 32.6% would look for a dentist for treatment of the cases. Only 15.8% would reimplant the tooth themselves. Regarding the storage media, 21.95% would keep the avulsed tooth in milk. There was no significant difference between gender and education level (p>0.05).
This study shows school teachers' lack of knowledge regarding dental trauma and especially tooth avulsion. Therefore, the results indicated that educational programs are necessary for improvement in their level of knowledge.
Journal of dentistry (Tehran, Iran). 01/2011; 8(3):117-22.
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Médecine et Maladies Infectieuses 12/2010; 41(2):112-4. · 0.72 Impact Factor
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ABSTRACT: Oral diseases, mainly tooth decay and periodontal disease, are among the most common conditions in the world. The tooth decay is very prevalent in developing countries and particularly in disadvantaged backgrounds where insecurity and its corollaries prevail. Thus, our study focused on one of these places: the prisons, characterized by deprivation of liberty, monotony, boredom, and the difficult conditions of detention, brief by a total dependence of the detainee. The aim of this study was to determine the DMFT (Decayed, Missing, Filled Teeth) in detainees dwelling in prisons in Dakar. This cross sectional study included 754 detainees aged 18 years and more with a stay exceeding more than a year, in one of the four main prisons of the capital city, Dakar. The majority of detainees was males (90%), aged between 18 and 35 years (63%) and was staying at "Camp penal" (65%). The proportion of inmates with at least one tooth cavity was 81.3%, those with teeth sealed was 26.1% and the prisoners with missing teeth reached 70.6%. The highest average DMFT was noted at "Camp penal" (7.3) among men (6.5) and among inmates aged 36 to 45 years (6.1) with a significant difference. The tooth decay is particularly worrying in the prison population, hence the urgency to establish a programme of prevention and care of oral diseases in this environment.
Odonto-stomatologie tropicale = Tropical dental journal 12/2010; 33(132):5-10.
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B Faye,
A L Bañuls,
B Bucheton,
M M Dione,
O Bassanganam,
M Hide,
J Dereure,
M Choisy,
J L Ndiaye,
O Konaté, [......],
I Sy,
A A Niang,
J F Molez,
K Victoir,
P Marty,
P Delaunay,
R Knecht,
S Mellul,
S Diedhiou,
O Gaye
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ABSTRACT: In the context of global warming and the risk of spreading arthropod-borne diseases, the emergence and reemergence of leishmaniasis should not be neglected. In Senegal, over the past few years, cases of canine leishmaniasis have been observed. We aim to improve the understanding of the transmission cycle of this zoonosis, to determine the responsible species and to evaluate the risk for human health. An epidemiological and serological study on canine and human populations in the community of Mont Rolland (Thiès area) was conducted. The data showed a high seroprevalence of canine leishmaniasis (>40%) and more than 30% seropositive people. The dogs' seroprevalence was confirmed by PCR data (concordance > 0.85, Kappa > 0.7). The statistical analysis showed strong statistical associations between the health status of dogs and seropositivity, the number of positive PCRs, clinical signs and the number of Leishmania isolates. For the first time, the discriminative PCRs performed on canine Leishmania strains clearly evidenced that the pathogenic agent is Leishmania infantum. The results obtained show that transmission of this species is well established in this area. That the high incidence of seropositivity in humans may be a consequence of infection with this species is discussed.
Microbes and Infection 12/2010; 12(14-15):1219-25. · 3.10 Impact Factor
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ABSTRACT: Oral diseases qualify as major public health problems due to their high prevalence and incidence. They usually affect poor and marginal segments of the population. This study focused on the prison population that is characterized by poor living conditions and malnutrition. The aim of study was to assess the need for periodontal care in prisoners serving time in the Dakar region of Senegal.
This cross-sectional study included 375 prisoners serving in three facilities, i.e. the penal camp, Liberte VI prison for women, and Rufisque prison for women. Data were collected using a modified WHO information sheet. The community periodontal index of treatment needs (CPITN) was used for scoring. Statistical analysis with stratification by sex and age was performed and tests were considered as significant for p < or = 0.05.
There were 335 men (89.3%) and 40 women (10.7%) with a sex ratio of 8.3. Mean age was 35 years +/- 11.5 (range, 16-88). Mean prison time was 39.29 months +/- 17.73. The proportion of prisoners needing periodontal treatment increased gradually according to prison time with significant differences (p < 0.05) even after stratification.
Better management taking into account the environment and prison time would reduce the need for periodontal treatment in prisons in Dakar.
Médecine tropicale: revue du Corps de santé colonial 12/2009; 69(6):587-90.
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ABSTRACT: The purpose of this descriptive study conducted in an area endemic for lymphatic filariasis was to update information on the prevalence, clinical aspects, and awareness of this parasitic disease. All consenting inhabitants over 10 years old in three selected sectors were included. An entomological investigation was carried out to estimate the rate of vector infection. Out of a total of 3.359 subjects examined, 57.3% reported familiarity with the disease and provided an accurate description. The prevalence of clinical manifestations, i.e., mainly adenopathy, attributable to the disease was 14.11%. The overall prevalence rate of parasites was 4.7% with significant variations between sectors. The only parasite species found was Wuchereria bancrofti. Parasite load was low with 68.8% of subjects having less than 10 microfilariae per microliter of blood. Data analysis identified the following risk factors: age with 77.2% of microfilariae carriers over the age of 45 years, sex with 61.4% of carriers being female, and duration of residence in endemic area with 80.4% of carriers living in the area for more than 10 years. Differences were statistically significant. Dissection of 511 female culicidae showed no microfilariae carriers. These data indicate a declining ten-dency in this endemic zone in Senegal.
Médecine tropicale: revue du Corps de santé colonial 03/2009; 69(1):101-2.
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ABSTRACT: Co-infection between intestinal parasites and Plasmodium falciparum is very frequent in inter tropical zone. Our study carried out in the North of Senegal (zone of high prevalence of schistosomiasis) aimed at measuring the influence of the carriage of intestinal parasites on the intensity of malaria infection. The Plasmodium falciparum densities were significantly higher during Plasmodium falciparum/Schistosoma mansoni and Plasmodium falciparum/Ascaris lumbricoides co-infection in children under 14 years old. Other intestinal parasites did not seem to have negative influence on the intensity of Plasmodium falciparum infection.
Bulletin de la Société de pathologie exotique 01/2009; 101(5):391-4.
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ABSTRACT: The aqueous extract of the Parinari excelsa barks at doses of 100 and 300 mg/kg/day for 7 days has a significant antihyperglycemic effect on alloxan-induced diabetic rats. At the same dose the acute oral administration of aqueous extract of the P. excelsa barks (100 and 300 mg/kg) induced a significant decrease of blood glucose on glucose-loaded normoglycaemic rats. Our results seem to confirm the rational bases for its use in traditional medicine.
Fitoterapia 07/2008; 79(4):267-70. · 1.85 Impact Factor
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ABSTRACT: Wrestling is a sport that is deeply-rooted in Senegalese cultural values. It is a popular sport that comes from local traditions but that has been influenced by boxing. This influence has made wrestling evolve to include the practice of boxing without gloves. Howeve, this practice causes many traumas in the mouth and teeth. The general objective of this study is to determine the frequency of the traumas among wrestlers who practice this form of wrestling associated with boxing. The specific objectives are to appreciate the bucco-dental conditions of the wrestlers, to evaluate the frequency and nature of the traumas in the intention to make recommendations and offer perspectives. 63.2% of wrestlers have at least presented a buckle or dental trauma. 28.8% of bucco-dental traumas occur among young wrestlers between 25 and 30 years and, 22.4% relate to the group between 19 and 24 years. 24.8% of bucco-dental traumas occur among wrestlers of the first category, followed by these of the second category with 21.6%, the fourth category with the lowest rate, 4% being last. It has been noted that 60% of buck-dental traumas occur in championship matches. 77.2% of dental and bucco traumas affect the upper jaw while. 22.8% affect the lower jaw. Affect teeth are often the group incisivo-canin. The National committee for the Management of wrestling in Senegal should make mandation the wearing of adapted gloves to attenuate the impact of blows. The Committee could also ban the inclusion of boxing practices in traditional wrestling and make compulsory the wearing of teeth protect in devices to minimize cases of bucco-dental traumas.
Odonto-stomatologie tropicale = Tropical dental journal 04/2008; 31(121):29-35.
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ABSTRACT: Shaping canal is one of the most important steps in endodontic treatment. It allows the cleaning solutions to reach the totality of the canal and to control any infection of the canal system. To achieve this goal is not without difficulty with the steel instruments, because of their rigidity. Recently, the Nickel Titanium instruments were introduced in Endodontics, to allow a setting form technically simplified and faster The aim of this study was to evaluate the duration of the canal shaping with the Pro Taper system.
This exploratory study was carried out in Conservative Odontology and Endodontics service (OCE) of the department of Odontology of Faculty of Medicine, Pharmacy and Odonto-Stomatology of the University CheikhAnta Diop of Dakar in 2006. Sixty nine root canals (50 teeth) were shaped with ProTaper.
The results showed that an average time of 17.35 minutes was necessary for the shaping of the long canals against 15.38 minutes for the middle canals and 14.29 minutes for the short canals. The average duration of canal shaping of the one-root teeth is 13.76 minutes and 17.77 minutes for the plural root teeth.
Taking into account the necessity of maintaining the teeth on the arcade during long year; include that we have now to respect imperatively the biological principles during the canal shaping in a restricted duration to realize a dense and hermetic canal obturation of the canal system in the same meeting. The Pro Taper system is so a reliable means.
Bulletin de la Société médicale d'Afrique noire de langue française 02/2008; 53(3):228-35.
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ABSTRACT: the toxoplasmosis can occur complications during pregnancy as serious repercussions on the foetus with the risks linked to this pathology such as abortion. However these complications can be avoided during the pregnancy by an early diagnosis. The objective of this study is to determine the prevalence of toxoplasmosis during pregnancy.
we undertook this study on 109 pregnant women addressed to the laboratory of parasitology and mycology of the CHU Dantec for toxoplasmosis serological test on 2002. We used a immunoenzymatic technical in solid phase whose principle rests on the change of coloration in the presence of antibody of IgM or IgG; coloration whose intensity is function of the title of antibody. For that, two serological tests (S1 and S2), starting from two venous blood at 3 weeks of interval, are carried out among these pregnant women. This second serology will make it possible to confirm or cancel an evolutionary toxoplasmosis based on the variation of the title in antibody between the first (S1) and the second serology (S2). A control is carried out among these women among whom the diagnosis of the evolutionary toxoplasmosis was established.
they show that with the first (S1), from the 109 patients, 3% were positive with the antibodies IgM type, 22% positive with IgG, and 11% positive with IgG and IgM. 36% of the women present a positive diagnostic at the first serology (S1). The second serology (S2) shows that among the 36% of the women diagnosed positive with the first serology, 11% made an evolutionary toxoplasmosis.
This study shows a rather significant of serological toxoplasmosis discovered fortuitously among pregnant women at the time of their assessment of pregnancy.
Bulletin de la Société médicale d'Afrique noire de langue française 02/2007; 52(1):58-61.
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ABSTRACT: Vascular endothelium possesses biological properties that are involved in important physiological functions such vascular permeability, vascular tone regulation and angiogenesis. The difficulty of culture and long-term maintenance of sufficient amount of normal endothelial cells has proven to be the limitation for the understanding of the biological function of the endothelium. Therefore, the aim of this study was to culture and characterize the porcine coronary endothelial cells.
The endothelial cells were isolated by collagenase treatment and cultured in culture dishes coated with collagen, prepared from rat tail, containing medium RPMI1640/M199 and 15% fetal calf serum supplemented with antibiotics and fungizon. The cells were maintained to grown at 37 degrees C. The medium was changed one day after and then every two day. The cells were incubated with Dil-labeled-acetylated-LDL for determination of acetylated-LDL uptake. Confluence cultures of cells were examined by phase-contrast and confocal microscopy.
After a day of culture, the endothelial cells adhere to the collagen and began to grow. While multiplying themselves, they colonize little by little the body of the surface of culture to form to confluence a monolayer of flat cells relatively homogenous. To confluence, the proliferation of the endothelial cells is inhibited by the contact and the cells present a polygonal aspect. Our results show that all the cultivated cells were strongly positive for acetylated-LDL markers. The endothelial cells, cultivated until the second passage corresponding to the second culture of the primary cultures, continued to present a good fluorescence.
Porcine coronary endothelial cells represent a useful in vitro model to study biological and physiopathological properties of vascular endothelium.
Bulletin de la Société médicale d'Afrique noire de langue française 02/2007; 52(1):27-30.
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ABSTRACT: Against cough activity from Guiera senegalensis' leaves has been previously scientifically studied. However, to the best of our knowledge, active principles responsible of this activity were not yet identified. Thus, we undertook this work with the aim to evaluate the effect of total alkaloids from plant's leaves in cough induced in guinea pig by ammoniac inhalation.
Guinea pigs were divided up in 5 groups of 10 and were crammed with distilled water (control), total alkaloids with 10, 25 and 30 mg/kg and codeine with 50 mg/kg. They were then exposed to ammoniac inhalations and the number of coughs was counted every hour, after cramming, during 5 min.
Our results show, after 5 hours, with the doses of 15, 25 and 30 mg/kg per os, that total alkaloids induce against cough activity, 2 hours after cramming (p < 0.01) and the difference was still significant 3, 4 and 5 hours after (p < 0.001). These alkaloids effects were comparable with codeine activity which was used as reference product.
Total alkaloids of Guiera senegalensis present an against cough activity and would be responsible of against cough activity of the plant.
Bulletin de la Société médicale d'Afrique noire de langue française 01/2007; 52(2):130-4.
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ABSTRACT: To evaluate the performance of bond strength and the compatibility of Prime &Bond NT self etching adhesive resins without activator on dentin surface with dual composite resin LuxaCore, and verify the contribution of a coactivator.
twenty-four human teeth without decay, molars or pre-molar are used. The dental crowning is cut with diamond disc mounted on a hand piece; the roof surface is flattened and well humidified. Each tooth is embedded in cold self-etching resin (Plexil Excil). Samples are divided into two groups of 12. in the first group, self-etching Prime &Bond NT resin adhesive without activator is supplied on the flat mesial dentin roots, then dual cure composite resin LuxaCore) is bonded. The second group was subject to the same treatment but with addition of co-activator the Prime &Bond NT self-etching adhesive resin. Debonding was achieved with Universal testing machine and mean shear bond strengths were determined for each test group. The data were subjected to ANOVA (p < 0001).
Bond strength obtained with self-etching Prime &Bond NT without activator is 10.635 MPa. With addition of activator it is 15.185 MPa
supplementing with an activator has significantly improved bonding strength.
Odonto-stomatologie tropicale = Tropical dental journal 12/2006; 29(116):30-4.
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ABSTRACT: This study aimed to investigate the mechanism of action of acetonic and hexanic extracts of the leaves of Vernonia colorata on blood glucose regulation.
Experiments were performed in normoglycaemic, type 1 and 2 diabetic rats. The effects of acetonic and hexanic extracts on blood glucose were evaluated. The antagonism effect of hexanic extract on the decrease of blood glucose induced with glibenclamide and metformin was also tested.
The hexanic extract of the leaves of V. colorata (HELVC) dose dependently increased the blood glucose in normoglycaemic rats. At the dose of 30 and 100 mg/kg, the glycaemia varied from 0.84 +/- 0.02 to 1.11 +/- 0.10 g/l and 0.68 +/- 0.02 to 1.31 +/- 0.30 g/l (p<0.05, n = 5). Such glibenclamide, the acetonic extract of the leaves of V. colorata (AELVC) induced hypoglycaemia in normoglycaemic rats. The HELVC prevents significantly the AELVC and glibenclamide induced hypoglycaemia. The chronic administration of the AELVC and HELVC as well as glibenclamide in type 1 diabetic rats did not change significantly the level of blood glucose. In type 2 diabetic rats, the single dose administration of metformin (300 mg/kg, per os) decreased the glycaemia which is completely prevented by a HELVC pretreatment.
These results suggest that: i) The respective hypo- and hyperglycaemic effects of AELVC and HELVC require the presence of the pancreatic beta cells. ii) The AELVC would act by a sulfonylurea-like mechanism as glibenclamide to induce an hypoglycaemic effect.
Bulletin de la Société médicale d'Afrique noire de langue française 01/2006; 51(1):42-6.
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ABSTRACT: The non carious cervical lesion (NCCL) is a loss of tooth tissue at the neck of affected teeth that is unrelated to tooth decay. They are commonly encountered in clinical practice and present in a variety of forms. The purpose of this paper is to determine the prevalence of the NCCL in a Senegalese population. From 655 patients, 112 with cervical lesions were identified i.e. a global prevalence of 17.10%. The prevalence rate for abrasion was reported to be 77.70%, 12.50% for abfraction and 9.80% for erosion. Etiological factors were studied for abrasion and erosion. 54% of the patients with abrasion used their toothbrush horizontally. For erosion, only external factors were identified: consumption of acidic drinks (9 patients) or alcohol (1 patient) and professional environment (1 patient). Dentists should consider these lesions in their daily practice.
Odonto-stomatologie tropicale = Tropical dental journal 01/2006; 28(112):15-8.
